CN101230362B - Method for effectively producing 1,3-propanediol by modifying permeability of cell membrane - Google Patents
Method for effectively producing 1,3-propanediol by modifying permeability of cell membrane Download PDFInfo
- Publication number
- CN101230362B CN101230362B CN2008100202031A CN200810020203A CN101230362B CN 101230362 B CN101230362 B CN 101230362B CN 2008100202031 A CN2008100202031 A CN 2008100202031A CN 200810020203 A CN200810020203 A CN 200810020203A CN 101230362 B CN101230362 B CN 101230362B
- Authority
- CN
- China
- Prior art keywords
- ammediol
- cell
- product
- concentration
- fermentation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to a method of effectively producing 1, 3-propylene glycol by changing the membrane permeability, which adds nonionics to change the membrane permeability, effectively promotes the cell growth, and increases the concentration of 1, 3-propylene glycol which is the finished product of fermentation. The utility model has the technical process that as follows: adding the second-class germ culture into the fermentation culture; adding nonionics which can improve the membrane permeability to the fermentation culture; the nonionics reduce the resistance of the oxygen and nutrient substance from entering the cell, promote the cell growth, enhance the secreting of metabolite to the extra-cellular area, reduce the accumulation of metabolite in cell, and facilitate the elimination of inhibition of product and sub product, in particular the inhibition of cell growth and cell catalytic activity by 1, 3-propylene glycol. The concentration of the fermentation product--1, 3-propylene glycol is increased by 10 to 70 percent. With simple technical process and low production cost, the invention is easy to be industrially applied.
Description
Technical field
The present invention is a kind of biotechnology preparation 1, and the method for ammediol is particularly added nonionogenic tenside and changed permeability of cell membrane, effectively improves 1, and the method for ammediol concentration belongs to 1, the biocatalysis synthesis technical field of ammediol.
Background technology
In organic synthesis, 1, ammediol has widely and uses as the bifunctional organic molecule.Its alternative terepthaloyl moietie, butyleneglycol as monomer and solvent, antifreezing agent or the protective material etc. of synthesizing polyester and urethane, also are used for synthetic medicine and are used as organic synthesis intermediate.
Its topmost purposes is the important monomer raw material of the excellent new type polyester fiber PTT (PTT) of manufacturing property.PTT is the high-grade clothing filamentary material with vegetable fibre characteristic of the mid-90 in 20th century commercial exploitation success.
PTT has excellent rebound resilience, dyeability, pollution resistance; And the biodegradable characteristic of natural circulation; Fully developing talents in fields such as carpet, engineering plastics, garment materials, be chosen as one of six big petrochemical industry product innovations by the U.S., is synthon hot of research and development in the world at present.Have only German Degussa company, U.S. Shell company and Du Pont company to realize 1 at present, the industriallization that ammediol is produced is predicted according to Schell company; Will be to world's ptt fiber demand in 2010 above 1,000,000 tons; When the time comes, to 1, the demand of ammediol also rises straight line.
1, the industrial process of ammediol mainly contains 3 kinds: oxyethane hydroformylation method, hydrolysis of acrolein method and microbe fermentation method.Production by Microorganism Fermentation 1; Ammediol is the metabolic reaction network that utilizes cell, makes reproducible biomass material efficiently be converted into 1, ammediol; Wherein transformation of glycerol approach particularly; Because glycerine is that biofuel is produced the main by product of institute's deutero-, also is the by product of ethanol fermentation simultaneously, and gets most of the attention.Existing a lot of research focuses on that optimizing microbial transformation glycerine produces 1, and the process of ammediol improves the yield-power of bio-reactor as adopting micro-aerobe fermentation technology; As being raising 1; The transformation efficiency of ammediol reduces the generation of by product, takes different fermentation control strategies respectively: technologies such as fed-batch, the cultivation of two stages, the fermentation of Double bottom thing, the fermentation of interpolation cofactor; Improve 1, the transformation efficiency of ammediol, output, reduction fermentation costs (1-6).
1、Chen?X，·Zhang?DJ，·Qi?WT，Gao?SJ，Xiu?ZL，.·Xu?P.Microbial?fed-batch?productionof?1，3-propanediol?by?Klebsiella?pneumoniae?under?micro-aerobic?conditions.Appl.Microbiol.Biotechnol.2003.63：143-146。
2, Zhang Yanping, Du Chenyu haves mercy on and controls Cao Zhuan. and vitamins C and E synthesize 1 to Klebsiella pneumoniae, the regulation and control of ammediol. process engineering journal .2005.5:197-200.
3, Cheng Keke, Sun Yan, Liu Weifang, Liu Dehua. bottoms stream adds strategy to fermentative Production 1, the influence of ammediol. food and fermentation industries .2004.30:398-40.
4, Chen Hongwen, Wang Wei, Fang Baishan, Hu Zongding. klebsiella spp produces 1, ammediol key enzyme Fermentation Conditions. the chemical engineering journal .2004.18:621-627 of colleges and universities.
5, Wu Bin, He Bingfang, Sun Zhihao, Ouyang Pingkai. butyric acid fusobacterium ferment glycerin preparation 1, the research of ammediol. industrial microorganism .2004.31:21-25.
6, Yang Dong, Li Chun, Du Chenyu, Zhang Yanping, Cao Zhuan. two sections Double bottom thing fermentative prodn 1, ammediol. process engineering journal .2003.3:269-273.
But all there is the inhibition of product, by product cell growth and catalytic activity of cells in existing technology, causes in the fermenting process biomass on the low side, and the production cycle is long, and production intensity is low.Tensio-active agent can improve permeability of cell membrane, in microbial fermentation, often is used to improve the output of title product.In fermentation process, add tensio-active agent; Can accelerate the material transfer transportation; Increase the outer material balance of born of the same parents in the born of the same parents, thereby improved the tolerance level of thalline, solve the inhibition of tunning, by product microorganism cells growth and catalytic activity to substrate, product.Therefore, in fermentation process, add tensio-active agent to increasing end product concentration, enhance productivity, shorten reaction time, reduce production costs, (7-9) has important practical significance.
7, Zhu Yan, Yuan Qipeng, Wang Hang. add the influence of oxygen carrier and tensio-active agent to the Lyeopene fermentation. microbiology circular .2006.33 (1): 90-93.
8, Wei Yi, Zhang Shurong, Liu Chunqiao is opened roc. add the influence of tensio-active agent to the fermentation of d-arbutin. chemical industry journal .2007.58 (9): 2352-2356.
9、Nemec?T，Jemej?c?K.Influence?of?Tween?80?on?lipid?metabolism?of?an?Aspergillusniger?strain.Appl?Biochem?Biotechnol.2002.101(3)：229-238。
Document is reported respectively and is added Span 20, Tween 80, Triton x-100, and Tween20, wherein β-Hu Luobusu is produced in the blakeslea trispora fermentation, and under top condition, the increasing degree of β-Hu Luobusu output can reach more than 15%; In the synthetic d-arbutin reaction of Xanthomonas campestris catalysis, add tensio-active agent, research is illustrated under the optimum reaction condition, and production concentration is blank high about 27.6%, and improves thalline to the tolerance level to the substrate dihydroxy-benzene, and fermentation period has shortened 12h; In the pectin lyase substratum of black mold, add Tween 80, can yield of enzyme be improved 70%.
Various and the character of kinds of surfactants has nothing in common with each other, and they have the permeability of cell membrane of improvement on the one hand, reduces the transmission resistance that oxygen and nutritive substance get into cell, accelerates the ability of material transfer transportation; Toxic and the germicidal action to mikrobe on the other hand.In general the toxicity of cats product pair cell is bigger; Nonionogenic tenside toxicity is little, the influence of pair cell also a little less than; The toxicity of AS and sterilizing power are situated between between the two.
Industrial fermentation produces 1, and ammediol mainly is an intestinal bacteria, and the cell growth produces pod membrane, and covers cell surface, and the viscosity of fermented liquid is along with the carrying out of fermentation increases gradually, and to dissolved oxygen and 1, the output of ammediol all produces very big influence.For 1, how the fermentation of ammediol guarantees that the transmission of oxygen and nutritive substance absorbs, and keeps the cell growth, and to further raising 1, the output of ammediol has great importance.
Summary of the invention
Technical problem: the object of the present invention is to provide a kind of change permeability of cell membrane effectively to produce 1; The method of ammediol; Increase the permeability of cytolemma through adding nonionogenic tenside, reduce the transmission resistance that oxygen and nutritive substance get into cell on the one hand, keep the cell growth; Impel microbial metabolites to be easy to secrete to born of the same parents on the other hand, be beneficial to and eliminate product, by product inhibition, particularly reduce 1, the inhibition of ammediol cell growth and catalytic activity of cells, thus help 1, ammediol synthetic method.
Technical scheme: method of the present invention is to add nonionogenic tenside to change permeability of cell membrane, reduces the transmission resistance that oxygen and nutritive substance get into cell, accelerates the ability of material transfer transportation, promotes the cell growth; Solve the inhibition of product, by product cell growth and catalytic activity of cells, improve tunning 1, ammediol concentration, its technological process is:
The secondary seed preparation: get 1, ammediol is produced bacterial classification, is inoculated in the seed culture medium, and 34-37 ℃, the 120-220rpm shaking table is cultivated 18-36h, inoculates in the seed culture medium, cultivates 12-16h for 34-37 ℃, and it is subsequent use to process secondary seed;
Fermentation culture: the 20-65g/L industry glycerol is done carbon source, liquid amount 40%-70%, and the inoculum size of 3%-15% is received the secondary seed nutrient solution wherein, and temperature 34-37 ℃, the 150-400rpm intermittent type is cultivated or the fed-batch formula is cultivated 20-40h; Cell concn and product 1, ammediol concentration are surveyed in the midfeather sampling; Begin to 24 hours in fermentation culture, add nonionogenic tenside; Cell concn and product 1 are surveyed in sampling at interval, ammediol concentration, and fermented liquid 1, the ammediol ultimate density improves 10%～70%.
1, ammediol is produced bacterial classification and is comprised: Cray uncle pneumobacillus, Fu Shi lemon bacterium, aerogenesis klebsiella, acid-producing Klebsiella bacterium.
Fermentative prodn 1, the nonionogenic tenside that adds in the ammediol process are Span or Tween or Triton or OP or AEO.
The mode that tensio-active agent adds is disposable adding or adds or stream adds concentration range 0.01～50g/L in batches.
The present invention, adopts and adds the permeability that nonionogenic tenside increases cytolemma in the ammediol fermenting process 1, reduces the transmission resistance that oxygen and nutritive substance get into cell on the one hand, keeps the cell growth; Impel 1 of generation on the other hand, ammediol is prone to secrete to born of the same parents, reduces by 1, and ammediol reduces 1 in intracellular accumulation, and the inhibition of ammediol pair cell catalytic activity solves product and suppresses, thereby helps 1, ammediol synthetic.
Beneficial effect: the zymotechnique that the invention provides simple and effective economy; Solution is fermented, and end product yields poorly and the inhibition of fermenting process product, by product cell growth and catalytic activity of cells; Effectively improve fermentation end product concentration; Enhance productivity, reduce production costs, be easy to realize industrial applications.
Embodiment
Involved 1, it can be Cray uncle pneumobacillus, Fu Shi lemon bacterium, aerogenesis klebsiella, acid-producing Klebsiella bacterium that ammediol is produced bacterial classification.
The present invention adopts and adds nonionogenic tenside change permeability of cell membrane, reduces the transmission resistance that oxygen and nutritive substance get into cell on the one hand, keeps the cell growth; Impel 1 of generation on the other hand, ammediol is prone to secrete to born of the same parents, reduces by 1, and ammediol reduces 1 in intracellular accumulation, and the inhibition of ammediol pair cell catalytic activity solves product and suppresses, and finally improves 1, ammediol output.The practical implementation process is:
The secondary seed preparation: get 1, ammediol is produced bacterial classification, is inoculated in the seed culture medium, and 34-37 ℃, the 120-220rpm shaking table is cultivated 18-36h, inoculates in the seed culture medium, cultivates 12-16h for 34-37 ℃, and it is subsequent use to process secondary seed.
Fermentation culture: the 20-65g/L industry glycerol is done carbon source, liquid amount 40%-70%, and the inoculum size of 3%-15% is received the secondary seed nutrient solution wherein, and temperature 34-37 ℃, the 150-400rpm intermittent type is cultivated or the fed-batch formula is cultivated 20-40h; Cell concn and product 1, ammediol concentration are surveyed in the midfeather sampling.Begin to 24 hours in fermentation culture, add nonionogenic tenside.The nonionogenic tenside that adds is Span or Tween or Triton or OP or AEO, concentration range 0.01～50g/L, and the mode that tensio-active agent adds is disposable adding, add or stream adds in batches.Cell concn and product 1, ammediol concentration are surveyed in sampling at interval.Fermented liquid 1, the ammediol ultimate density improves 10%～70%.
1, ammediol is produced bacterial classification and is comprised: Cray uncle pneumobacillus, Fu Shi lemon bacterium, aerogenesis klebsiella, acid-producing Klebsiella bacterium.
The glass or plastic containers Span or Tween or Triton or OP or the AEO that add, concentration range 0.01～50g/L.
The mode that tensio-active agent adds is that disposable adding or stream add.
Below be specific examples:
Bacterial classification: Cray uncle pneumobacillus (Klebsiella pneumoniae)
Solid slant culture base: Carnis Bovis seu Bubali cream 3g/L, MgSO
47H
2O 0.2g/L, peptone 5g/L, NaCl 10g/L, agar 20g/L, glycerine 20g/L.
Seed culture medium: yeast extract paste 2g/L, MgSO
47H
2O 0.2g/L, Hydrocerol A 0.42g/L, K
2HPO
41.6g/L, K
2HPO
43.4g/L, NH
4Cl 3.0g/L, glycerine 30g/L, CaCl
20.5g/L.
Fermention medium: glycerine 20-65g/L, K
2HPO
43H
2O 1.36g/L, NH
4Cl 3.0g/L, yeast extract paste 2.0g/L, MgSO
47H
2O 0.2g/L, CaCl
22.0g/L ferrous solution 1mL/L, inorganic ionic solution 2mL/L, V
B12Solution 1mL/L, cobalt liquor 1mL/L.Mineral ion contains: CuCl
2, H
3BO
3, MnCl
2, Na
2MoO
4, NiCl
2, ZnSO
4, micronutrient levels is about 0.01～50mg/L.
The secondary seed preparation: get the fresh solid slant strains of Cray uncle pneumobacillus, be inoculated in the seed culture medium, 35 ℃ of shaking tables, 150rpm cultivates 24h, inoculates in the seed culture medium, cultivates 12-16h for 35 ℃, and it is subsequent use to process secondary seed.
Fermentation culture: the 20-65g/L industry glycerol is done carbon source, liquid amount 40%-70%, and the inoculum size of 3%-15% is received the secondary seed nutrient solution wherein, and temperature 34-37 ℃, the 150-400rpm intermittent type is cultivated or the fed-batch formula is cultivated 20-40h; Cell concn and product 1, ammediol concentration are surveyed in the midfeather sampling.At fermentation culture 4-24 hour, add nonionogenic tenside, cell concn and product 1, ammediol concentration are surveyed in sampling at interval.
The glass or plastic containers Span or Tween or Triton or OP or the AEO that add, concentration range 0.01～50g/L.The mode that tensio-active agent adds is that disposable adding or stream add.
Embodiment one
The fermention medium that 400ml contains the 45g/L industry glycerol places 1L to shake bottle, inserts volume 40ml secondary seed, 37 ℃ of temperature, and shaking table 180rpm cultivates.Add respectively at fermentation 8h that concentration is 0%, 0.05%, 0.1%, 0.5%, 0.6%, 0.8%, 1%OP-10, sampling at set intervals once, the spectrophotometer turbidimetry is measured cell concn, wavelength 650nm, cell concn are that biomass is expressed as OD; Gas chromatographic analysis 1, the concentration of ammediol.Finished fermentation in 24 hours.
Embodiment one has studied at ferment glycerin and has produced 1, in the ammediol technology, adds nonionogenic tenside OP-10, and concentration is in 0～1.0% scope, and different OP concentration are to Cray uncle pneumobacillus growth and 1, the influence of ammediol output.Fig. 1 shows that OP-10 mainly shows as the promoter action to biomass when lower concentration, then mainly shows as the toxic action of pair cell when concentration is high, and biomass descends.Fig. 2 show OP-10 when lower concentration to 1, the raising of ammediol output has promoter action, increasing degree can reach more than 16%.
Analyze above result's reason, add the fermentating culturing process of OP-10, OP has improved the transmission of nutritive substance and oxygen, promotes the cell growth; Impel 1 of generation on the other hand, ammediol is prone to secrete to born of the same parents, reduces by 1; Ammediol reduces 1, the inhibition of ammediol pair cell catalytic activity in intracellular accumulation; The solution product suppresses, final biomass and 1, and ammediol output is improved.But along with the raising of OP concentration, OP also shows certain toxic action to mikrobe.The optimum concn dosage of OP-10 effect is 0.05～0.5%, i.e. 0.5～5g/L.
Embodiment two:
The fermention medium that 400ml contains the 50g/L industry glycerol places 1L to shake bottle, inserts volume 40ml secondary seed, 37 ℃ of temperature, and shaking table 180rpm cultivates.Adding concentration respectively at fermentation 4h, 8h, 12h, 16h is 0.1%OP-10, and 24 hours finish to ferment.Gas chromatographic analysis 1, the concentration of ammediol.
Table 1 fermentation 4h.8h.12h.16h adds bottle numbering of shaking that concentration is 0.1%OP-10 respectively
Can see that from Fig. 3 add OP to 1 at the fermentation different times, the output of ammediol has considerable influence, best 8-16 hour adding effect, 1, the output maximum of ammediol has increased by 12%.
Can prove through above-mentioned experiment, produce 1 at glycerol fermentation, in the ammediol technology; Increase the permeability of cytolemma through adding nonionogenic tenside, can effectively promote the cell growth, improve fermentation end product 1; The method of ammediol concentration, the easy economy of technology.
Claims (2)
1. one kind changes effectively production 1 of permeability of cell membrane; The method of ammediol is characterized in that adding nonionogenic tenside and changes permeability of cell membrane, reduces the transmission resistance that oxygen and nutritive substance get into cell; Accelerate the ability of material transfer transportation, promote the cell growth; Solve the inhibition of product, by product cell growth and catalytic activity of cells, improve tunning 1, ammediol concentration, its technological process is:
The secondary seed preparation: get 1, ammediol is produced bacterial classification, is inoculated in the seed culture medium, and 34-37 ℃, the 120-220rpm shaking table is cultivated 18-36h, inoculates in the seed culture medium, cultivates 12-16h for 34-37 ℃, and it is subsequent use to process secondary seed;
Fermentation culture: the 20-65g/L industry glycerol is done carbon source, liquid amount 40%-70%, and the inoculum size of 3%-15% is received the secondary seed nutrient solution wherein, and temperature 34-37 ℃, the 150-400rpm intermittent type is cultivated or the fed-batch formula is cultivated 20-40h; Cell concn and product 1, ammediol concentration are surveyed in the midfeather sampling; Begin to 24 hours in fermentation culture, add nonionogenic tenside; Cell concn and product 1 are surveyed in sampling at interval, ammediol concentration, and fermented liquid 1, the ammediol ultimate density improves 10%～70%;
1, ammediol is produced bacterial classification and is comprised: Cray uncle pneumobacillus,
Fermentative prodn 1, the nonionogenic tenside that adds in the ammediol process are OP-10.
2. change permeability of cell membrane according to claim 1 effectively produces 1, and the method for ammediol is characterized in that mode that tensio-active agent adds is disposable adding or adds or stream adds concentration range 0.01～50g/L in batches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100202031A CN101230362B (en) | 2008-02-27 | 2008-02-27 | Method for effectively producing 1,3-propanediol by modifying permeability of cell membrane |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100202031A CN101230362B (en) | 2008-02-27 | 2008-02-27 | Method for effectively producing 1,3-propanediol by modifying permeability of cell membrane |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101230362A CN101230362A (en) | 2008-07-30 |
| CN101230362B true CN101230362B (en) | 2012-07-04 |
Family
ID=39897175
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100202031A Expired - Fee Related CN101230362B (en) | 2008-02-27 | 2008-02-27 | Method for effectively producing 1,3-propanediol by modifying permeability of cell membrane |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101230362B (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102071225A (en) * | 2010-11-23 | 2011-05-25 | 安徽立兴化工有限公司 | Method for producing 1,3-propylene glycol through fermentation by improving cell enzymatic activity |
| CN104561134B (en) * | 2013-10-22 | 2019-06-14 | 中国石油化工股份有限公司 | A kind of method of Production by Microorganism Fermentation 1,3- propylene glycol |
| CN106164344B (en) * | 2014-02-07 | 2018-12-18 | 韩国生命工学研究院 | The method to interact between protein nano granulin molecule is measured using flow cytometer |
| CN104140984A (en) * | 2014-08-04 | 2014-11-12 | 杭州宝晶生物股份有限公司 | Method for producing L(+)-tartaric acid by improving cell permeability |
| CN106566847A (en) * | 2016-10-17 | 2017-04-19 | 北京工商大学 | Protective agent for improving tolerance of Klebsiella pneumoniae to 1, 3-propylene glycol and increasing production capacity |
| CN107475312B (en) * | 2017-09-14 | 2021-05-18 | 中国科学院合肥物质科学研究院 | Method for effectively producing vitamin K2 by improving cell membrane permeability |
| CN108624630A (en) * | 2018-05-15 | 2018-10-09 | 日照金禾博源生化有限公司 | A method of shortening the citric acid fermentation period |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1348007A (en) * | 2001-04-27 | 2002-05-08 | 大连理工大学 | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol |
-
2008
- 2008-02-27 CN CN2008100202031A patent/CN101230362B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1348007A (en) * | 2001-04-27 | 2002-05-08 | 大连理工大学 | Microbial micro-aerobe fermentation process of producing 1,3-propylene glycol |
Non-Patent Citations (5)
| Title |
|---|
| 任石苟等.表面活性剂在发酵工业中的应用.《化学与生物工程》.2006,第23卷(第6期),50-52. * |
| 杨东.发酵法生产1 3-丙二醇的高产策略.《精细与专用化学品》.2002 |
| 杨东.发酵法生产1,3-丙二醇的高产策略.《精细与专用化学品》.2002,(第20期),15-18. * |
| 王剑锋等.生物转化生产1 3-丙二醇的研究进展.《化学通报》.2001 |
| 王剑锋等.生物转化生产1,3-丙二醇的研究进展.《化学通报》.2001,(第10期),621-625. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101230362A (en) | 2008-07-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101230362B (en) | Method for effectively producing 1,3-propanediol by modifying permeability of cell membrane | |
| Ma et al. | Open fermentative production of L-lactic acid with high optical purity by thermophilic Bacillus coagulans using excess sludge as nutrient | |
| Cheng et al. | 1, 3-Propanediol production by Klebsiella pneumoniae under different aeration strategies | |
| Liu et al. | Microbial production of 1, 3-propanediol from glycerol by Klebsiella pneumoniae under micro-aerobic conditions up to a pilot scale | |
| Yang et al. | Fermentation of biodiesel-derived glycerol by Bacillus amyloliquefaciens: effects of co-substrates on 2, 3-butanediol production | |
| CN102643770B (en) | Colibacillus capable of generating succinic acid by anaerobic growth in synthetic medium pure and application thereof | |
| CN101792778B (en) | Method for fermentation production of succinic acid by circulating utilization of recombinant Bacillus coli cells | |
| CN101285047B (en) | D-lactic acid-producing strain with high optical purity and process for producing D-lactic acid by fermentation thereof | |
| CN102559782B (en) | Process for producing butyric acid by using bagasse hydrolysate through clostridium tyrobutyricum fermentation | |
| CN101319242A (en) | Method for preparing bacteria cellulose with association of activity and inertia | |
| CN106399204B (en) | Microbial mixed flora for producing 1, 3-propylene glycol by fermentation and fermentation method | |
| CN102864113A (en) | Strain capable of producing succinic acid, method for producing succinic acid and application thereof | |
| CN103952447A (en) | Method for producing succinic acid by virtue of fermentation under anaerobic conditions | |
| CN102352382B (en) | Method producing malic acid through double-phase fermentation | |
| CN101851642B (en) | Method for producing 1,3-propylene glycol by micro aerobic-anaerobic continuous flowing glycerol-adding multi-tank series fermentation | |
| CN102747114B (en) | Method for regulating recombinant escherichia coli metabolism by using transient anaerobic fermentation | |
| CN102864177B (en) | Method for promoting fermentation of microorganism to produce 1,3-propylene glycol | |
| CN101323863B (en) | Method for improving concentration of 1,3-propanediol produced by microbial fermentation | |
| CN110643525B (en) | Clostridium butyricum strain for producing 1, 3-propylene glycol and screening method and application thereof | |
| CN104561139A (en) | Method for increasing final cell density of microorganisms and shortening culture time | |
| CN103320367A (en) | Screening method and application of Escherichia coli for preparing high-yield succinic acids by utilizing synthetic media under anaerobic conditions | |
| CN102936575B (en) | Escherichia coli LL016 and application thereof | |
| CN103937733B (en) | Genetic engineering strain utilize sucrose to produce succinic acid from and method for production of succinic acid by fermenting the same | |
| CN103320366B (en) | Screening method and application of Escherichia coli for preparing high-yield succinic acids by utilizing synthetic media under anaerobic conditions | |
| CN102492746B (en) | Method for co-producing gamma-polyglutamic acid and glutamic acid by Bacillus licheniformis fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120704 Termination date: 20150227 |
|
| EXPY | Termination of patent right or utility model |