CN101222846A - Enhanced tuburculocidal activity and decreased fumes from glutaraldehyde disinfectant using acetate salts and alcohol - Google Patents

Enhanced tuburculocidal activity and decreased fumes from glutaraldehyde disinfectant using acetate salts and alcohol Download PDF

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CN101222846A
CN101222846A CNA200680025411XA CN200680025411A CN101222846A CN 101222846 A CN101222846 A CN 101222846A CN A200680025411X A CNA200680025411X A CN A200680025411XA CN 200680025411 A CN200680025411 A CN 200680025411A CN 101222846 A CN101222846 A CN 101222846A
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alcohol
glutaraldehyde
disinfectant
acetate
percent
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CN101222846B (en
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诺曼·A·迈纳
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Healthpoint Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
    • A01N35/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aliphatically bound aldehyde or keto groups, or thio analogues thereof; Derivatives thereof, e.g. acetals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof

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  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)

Abstract

The addition of alcohol plus acetate salts unexpectedly enhanced the tuberculocidal activity of glutaraldehyde, and decreased the fumes of glutaraldehyde from the formula of a high-level disinfectant.

Description

Use acetate and alcohol to obtain the raising of glutaraldehyde disinfectant mycobacterium bactericidal activity and the minimizing of smell
Technical field
The present invention is day to be the improvement of the United States Patent (USP) 5,863,547 on January 26th, 1999 to authorizing.Recent findings in order to reach the glutaraldehyde high effect disinfectants killed mycobacterium (TB), Gram-positive and Gram-negative vegetative bacteria, fungi and virus under 20 ℃ in 10 minutes, is necessary to add simultaneously pure and mild acetate.In addition, the combination of pure and mild acetate can significantly suppress the smell of glutaraldehyde.The result who does like this is, be used for to reusable medical, the dentist of thermal sensitivity with and the animal doctor carry out the prescription kill bacteria and produce less smell more quickly of the improved glutaraldehyde of high-effective disinfecting and/or sterilization with utensil.I am that the application is incorporated in the disclosure of being done in the purpose United States Patent (USP) 5,863,547 January 26 in 1999 by reference into authorizing day.
Background technology
Many medical apparatus all are that polymeric material, glue, glass lens and the electronic component with thermal sensitivity constitutes.Such utensil for example has OGD, Sigmoidoscope, cystoscope, arthroscorpe, process is esophagus and detector vagina and anesthesia and respiratory therapy apparatus.These thermal sensitivity utensils are very expensive, therefore normally use the back that another patient is reused a patient, and can not be with steam or xeothermic carrying out disinfection.Therefore, these thermal sensitivity utensils use the most efficiently that the aqueous chemical disinfectant carries out disinfection.High effect disinfectants can kill Gram-positive and Gram-negative vegetative bacteria, mycobacterium such as Much's bacillus, fungi and various types of virus in short relatively open-assembly time, and can kill a large amount of bacterial spore that becomes dry from the teeth outwards in longer open-assembly time.
The high effect disinfectants chemical reagent of medical apparatus of can be used for sterilizing has glutaraldehyde, other aldehydes, as o-phthalaldehyde(OPA) and formaldehyde, Peracetic acid, hypochlorous acid and chlorine dioxide.These chemical reagent all have serious defective as high effect disinfectants.Kill the measurement that spore test 966.04 (Association of Official Analytical Chemists (AOAC) Sporicidal Test966.04) carry out according to analytical chemistry association of official (AOAC), glutaraldehyde needed about 45 minutes to kill 10 under 25 ℃ 6Mycobacterium, glutaraldehyde needs to come in about 10.0 hours the killing bacteria spore under 25 ℃.Such open-assembly time and temperature all are unpractical, can be reduced artificially usually in practical operation.Glutaraldehyde has strong smell and sensitization problem, therefore needs special equipment to prevent that smell from leaking and discharging.And formaldehyde is a kind of carcinogen with noxious odor.The vapor phase of o-phthalaldehyde(OPA) is to insipidness, but its steam can make patient and medical personnel produce allergy.Some patients and medical personnel produce irritated to o-phthalaldehyde(OPA), and reaction is worked as repeated exposure in the smell that they can't smell for anaphylactic shock.Kill spore test 966.04 according to AOAC, o-phthalaldehyde(OPA) need be come the killing bacteria spore in about 32 hours.The dissolubility of o-phthalaldehyde(OPA) is relatively poor relatively, therefore is difficult to it is washed off from the surface.Aldehydes can use and reuse 14 to 30 days usually.Peracetic acid has the smell of stimulation, must be contained in the device, and product uses under 50 ℃-56 ℃ temperature.Can damage some colloidal substances and polymeric material in 50 ℃-56 ℃ oxidisability Peracetic acid of using down of higher temperature.All oxidative chemistries for example Peracetic acid, hypochlorous acid and chlorine dioxide are all unstable, are disposable therefore or the product of use on the one.
Therefore need a kind of disinfectant efficiently, the environmental temperature that it can allow in practical operation and carrying out disinfection in open-assembly time, and safety and nothing perceive smell, and can use and reuse a couple of days.For this purpose, my United States Patent (USP) formerly is the improvement of the first step.
Before, we find that the alcohol of relatively low concentration has improved the activity to mycobacterium (United States Patent (USP) 5,863,547) of glutaraldehyde.Yet this patent is but clearly instructed and is avoided adding acetate (the 2nd hurdle, 28-30 is capable).Further research has now found that, in conjunction with pure and mild acetate to temperature as 20 ℃ down and the activity of very rapidly optimizing glutaraldehyde in attainable open-assembly time to mycobacterium be necessary.Further astonishing discovery is that because the existence of pure and mild a certain amount of acetate, the smell of glutaraldehyde reduces greatly.
After adding acetate, the pH value of non-activated glutaraldehyde solution increases to about 6.5.The stable p H value of glutaraldehyde is about 3.5-4.5.Because the pH value is 6.5 o'clock, the concentration of non-activated glutaraldehyde can slowly reduce in 9-12 month storage time.Therefore, it is about 3.5% for example to be necessary to make the concentration of the glutaraldehyde of beginning to maintain, and the concentration after back glutaraldehyde between 12 months storage life is using and reusing 14 days is at least 2.0% like this.Reuse the concentration that disinfectant can dilute glutaraldehyde unintentionally, such as since the wet utensil that had just cleaned some water are brought in the disinfectant, and the utensil of just having sterilized with some glutaraldehydes washed off.In addition, because unintentionally dilution meeting takes place during 14 days use and reuse, therefore use higher concentration when being necessary to begin, promptly reach 26% alcohol, like this because the determining alcohol after using and reuse the dilution unintentionally that causes can be about 15%.Acetate must have and is about 8% higher initial concentration, still can keep like this to be about 5% minimal effective concentration after using and reuse 14 days.The valid density meaning used herein is the concentration after using and reuse 14 days.
Main purpose of the present invention is that the prescription of United States Patent (USP) formerly 5,863,547 that I am had carries out the improvement of following several importances.At first, improve rate of anti-microbial kill; The second, improve prescription, even make it use or reuse as still keep minimal effective concentration after 14 days; The 3rd, by the acetate of adding 3%-8%, thus the efficient of raising kill rate and disinfectant; The 4th, by making up existing acetate and alcohol, reduce the pungent smell of glutaraldehyde out of a clear sky.
In the following description of this invention, can clearly realize the method or the mode of this main purpose and other purposes.
Summary of the invention
The invention describes high-level disinfectant formulation, this prescription can be sterilized at ambient temperature fast to the thermal sensitivity medical apparatus, for example finishes in 10.0 minutes, and has appreciable but relatively low glutaraldehyde smell.This prescription comprises glutaraldehyde (2.0%-5.0%), and alcohol (5%-26%), and acetate (percentage by weight 3%-percentage by weight 8%), and they all cushion with the alkaline buffer system of pH value at 7.3-7.9 in use.This can make glutaraldehyde stable in during 14 days use and reuse.Kill spore test 966.04 according to AOAC, this prescription is also can be at 20 ℃ of killing bacteria spores in following 6.0 hours under the poorest concentration.The smell of glutaraldehyde is reduced to 75%, and this is not because the reduction of glutaraldehyde concentration causes, but because the existence of pure and mild acetate generation.These discoveries provide the improved high-level disinfectant formulation that can sterilize under time of reality permission and temperature.
Embodiment
Glutaraldehyde is first kind of composition in the composition, and its initial amount is between the about 2.0%-5.0% of percent by volume.Unless otherwise noted, represented here percentage range all is percent by volume scopes.Preferably the percent by volume of the initial concentration of glutaraldehyde is 3.5%, and the concentration of glutaraldehyde can still can maintain 2.0% or higher between the storage life and after using and reuse 14 days like this.Acetate will improve the storage pH value of prescription to about 6.5.Glutaraldehyde is the most stable when the pH value is about 3.5-4.5.During to surpass about 12 months pH be 6.5 storage, the concentration of glutaraldehyde will reduce gradually.Therefore, be necessary to begin, thereby still can keep effective least concentration after can and using and reuse 14 days during storing in a warehouse with the glutaraldehyde of higher concentration.Glutaraldehyde provides topmost antibacterial activity for composition.
Alcohol is second kind of composition in the composition.Be applicable to that alcohol of the present invention is the alcohol of the Yi Yushui mixing of straight chain and side chain, comprises methyl alcohol, ethanol, isopropyl alcohol and other alcohol.Serve as preferred wherein with isopropyl alcohol and ethanol.
The concentration of alcohol exists to about 26% with percent by volume about 5%.The volumetric concentration of preferred alcohol is 24-26%.This determining alcohol has greatly strengthened as 2.0% the glutaraldehyde bactericidal activity to mycobacterium.Concentration is the no mycobacterium bactericidal activity of alcohol of percent by volume 5%-20% itself, and the glutaraldehyde of percent by volume 2.0% does not have the mycobacterium bactericidal activity down and in 10 minutes practicable open-assembly time at 20 ℃ yet.Yet, concentration be percent by volume 5.0%-26.0% alcohol with but can kill mycobacterium fast in following 10 minutes as the combination of the glutaraldehyde of percent by volume 2.0% at 20 ℃.And acetate will further strengthen the bactericidal activity of glutaraldehyde to mycobacterium, thereby the glutaraldehyde concentration in the prescription can further reduce.
Second kind of composition in the composition is preferably isopropyl alcohol, and its initial amount is preferably between the about 24%-26% of percent by volume.It is stable that isopropyl alcohol will keep in during storing in a warehouse.After disinfectant uses or reuses 14 days, some alcohol will volatilize, and other alcohol will be brought into the water in the disinfectant by wet utensil and by mistake dilute, and the equipment of just having sterilized will have alcohol and be rinsed.Therefore, adopt 26% initial volume alcohol according to appointment when being necessary to begin, still can keep at least 15% alcohol behind the disinfectant using or reuse 14 days like this.The combination of preferred isopropyl alcohol and acetate has greatly strengthened the bactericidal activity of glutaraldehyde to mycobacterium, and alcohol has also suppressed composition owing to adding the foam that surfactant produces.
With potassium acetate or sodium acetate is the third composition that preferred acetate is composition, exists with the about 3%-percentage by weight 8% of percentage by weight at first.Acetate is stable during storing in a warehouse.Be necessary to begin, still can keep at least 5% preferred minimum level of significance behind the disinfectant using or reuse 14 days like this with the acetate of percentage by weight about 8%.Acetate has greatly strengthened the bactericidal activity of glutaraldehyde to mycobacterium with the combination of alcohol.But acetate is the spore activity extremely of enhancing composition also.Acetate and the pure smell that has together suppressed glutaraldehyde unexpectedly, this point is undoubtedly desired.
The 4th kind of composition of this sterilised formula is buffer, is preferably phosphate buffer.Can stably there be about 14 days in glutaraldehyde during using and reusing in being buffered to the said composition of about pH7.3-7.9.Adopt the buffer outside the phosphate,, will cause glutaraldehyde concentration to reduce about 40% as with heavy carbonate buffer disinfectants activated.So phosphate is preferred.For said composition, it is of great value keeping the concentration of the glutaraldehyde of activation during 14 days use and reuse.Than the composition with other buffers buffering, this chemical stability during 14 days use and reuse will provide the glutaraldehyde that more has antibacterial activity in preset time.The amount of buffer is from 4g/l-7g/l.
As in the composition of the patent before me, preferably containing surfactant in the composition.The level of preferred surfactants is a percentage by weight 0.0025%-percentage by weight 0.01%.The surfactant that is fit to not is essential, be basically with list in my patent formerly in the surfactant that uses identical, this patent is here incorporated into by reference.
Generally speaking, activate with phosphate buffer, the glutaraldehyde that contains 2%-5%, the alcohol of 5%-26%, the acetate of percentage by weight 3%-percentage by weight 8%, the combination of the nonionic surface active agent of the low foaming of percentage by weight 0.0025%-percentage by weight 0.01% provides disinfectant efficiently, it kills the microorganism of all non-formation spores at ambient temperature in 10.0 minutes, kill the bacterial spore in the culture medium that becomes dry from the teeth outwards at ambient temperature in 6.0 hours, thereby have no pungent but can perceive evitable smell, but and this disinfectant safety economy ground be used for the thermal sensitivity device and reach 14 days.Therefore, purpose of the present invention is achieved.
The example that provides has below further carried out nonrestrictive description to the present invention.Self-evident, within the spirit and scope of the present invention, can the scope of the composition of composition and adding be changed.In other words, following examples only are descriptive, do not constitute limitation of the scope of the invention.
Embodiment 1
Representative formula of the present invention is performed as follows mixing:
Glutaraldehyde isopropyl alcohol non-ionic surface active agent Na 2HPO 4Buffer acetic acid salt solution To percent by volume 3.2% percent by volume 26% percentage by weight 0.01% 7.4pH percentage by weight 8% surplus
This prescription is used for embodiment 2.
Embodiment 2
Present embodiment shows alcohol (being preferably isopropyl alcohol) and acetate is necessary for the bactericidal activity of optimizing mycobacterium.
In the research, prepared the multiple prescription that contains and do not contain active component isopropyl alcohol and potassium acetate, and tested them and killed the ability of killing mycobacterium bovis mutation BCG (Mycobacterium bovis var.BCG) under the suspension test rate (rate of kill suspension test).Kill the BCG under the suspension test rate.Mycobacterium bovis mutation GCG (M.bovis var.GCG) culture of 5.0ml that will contain the heat-inactivated calf serum of 5% (v/v) is in the prescription of 20 ℃ of embodiment 1 that down add to advance 45.0ml.At 20 ℃ down after 2.5,5.0,7.5 and 10.0 minutes, take out the reactant mixture of 1.0ml respectively in the above moment, in 9ml He in the recovery media, make ten times of continuous dilutions.With the filter opening size is that 0.45 micron film filter filters dilution, and washes with deionized water.Filter is placed into 35 ± 2 ℃ accompanying 3-4 week on the M7H9 agar in the Ti Shi culture plate.Number goes out the number of bacterium colony, and multiply by the suitable dilution coefficient to determine survival colony forming unit (CFU) number in the reaction flask in given exposure constantly.Can measure by same mode after 5.0,10.0,20.0 and 30.0 minutes in exposure under 25 ℃ by the commercial material C idex  that buys a kind of.
In above-mentioned two researchs, the recipe ratio that contains the potassium acetate of 2.4% glutaraldehyde, 15% isopropyl alcohol and 5% of the present invention comprises that other prescriptions of Cidex  can kill mycobacterium bovis mutation BCG quickly.1.5 times of same recipe dilutions are contained 1.6% the glutaraldehyde of having an appointment, 10% isopropyl alcohol and 3.33% potassium acetate, the prescription of this dilution occupies second on the speed of killing mycobacterium bovis mutation BCG.The prescription of isopropyl alcohol (no potassium acetate) that contains 2.4% glutaraldehyde and 15% on the speed of killing mycobacterium bovis mutation BCG slightly faster than the prescription and the Cidex  that contain 2.4% and 5% potassium acetate (no isopropanol).
In above-mentioned two researchs, prescription of the present invention (all concentration with diluted 1.5 times) contains isopropyl alcohol and two kinds of components of potassium acetate simultaneously, and it all shows better effect compared with the prescription and the Cidex  that one of lack in these two kinds of components.This shows that isopropyl alcohol and potassium acetate are the requisite composition of lethality that greatly strengthens mycobacterium bovis mutation BCG.
For the steps necessary that obtains conclusion among above-mentioned this embodiment 2 as follows:
The preparation of mycobacterium bovis mutation BCG
Test at this and to obtain mycobacterium bovis mutation BCG in preceding 12 months.The culture of mycobacterium bovis mutation BCG was grown 21-25 days on the M7H9 agar slant in the test tube of 25 * 250mm screw lid under 35 ± 2 ℃.The female culture that produces is stored in 3 ± 2 ℃ the refrigerator and is used for test.Broth culture stirs in vortex agitator, and spares matter 5-10 time in the homogenizer organizing of 40ml.One (1) part of heat-killed calf serum joined in 19 parts the culture (ultimate density is 5% (v/v)).
The preparation of invention prescription
The following prescription of preparation is also tested:
(1) 2.4% glutaraldehyde, 15% isopropyl alcohol, 5% potassium acetate, 0.001% mill indigo plant (keyacidblue), 0.0025% Laureth-23, Q.S. is to the distilled water of 100ml.Activate, use NaH with yellow #5 2PO 4And Na 2HPO 4Regulate about pH to 7.60.
(2) 2.4% glutaraldehyde, 15% isopropyl alcohol, 0.001% mill indigo plant (keyacid blue), 0.0025% Laureth-23, Q.S. is to the distilled water (no potassium acetate) of 100ml.Activate, use NaH with yellow #5 2PO 4And Na 2HPO 4Regulate about pH to 7.60.
(3) 2.4% glutaraldehyde, 5% potassium acetate, 0.001% mill indigo plant (keyacid blue), 0.0025% Laureth-23, Q.S. is to the distilled water (no isopropanol) of 100ml.Activate, use NaH with yellow #5 2PO 4And Na 2HPO 4Regulate about pH to 7.60.
(4) 2.4% glutaraldehyde, 15% isopropyl alcohol, 5% potassium acetate, 0.001% mill indigo plant (keyacidblue), 0.0025% Laureth-23, Q.S. is to the distilled water of 100ml.Activate, use NaH with yellow #5 2PO 4And Na 2HPO 4Regulate about pH to 7.60.With synthetic hard water dilution 2+1.(1.6% glutaraldehyde, 10% isopropyl alcohol, 3.33% potassium acetate).
Mycobacterium bovis mutation BCG is exposed to prescription (1)-(4)
The selected prescription of 45.0ml is joined in Erlenmeyer (Erlenmeyer) flask of 250ml, and put into 20 ± 1 ℃ water-bath.Add the mycobacterium bovis mutation BCG of the 5.0ml that contains the heat-killed calf serum of 5.0% (v/v), and make solution become the whirlpool shape to mix.Expose 2.5,5.0,7.5 down at 20 ± 1 ℃, after 10.0 minutes, in the above disinfectant/culture solution of constantly taking out 1.0ml respectively that exposes,, make ten times of continuous dilutions with among the Dey-Engley that contains 1% carbohydrate gum of its adding 9ml part and in the recovery media.With the filter opening size is that 0.45 micron film filter filters dilution, and washes with the aseptic deionized water about 50ml (SDIW).Filter is placed on the M7H9 agar of accompanying the Ti Shi culture plate.To coil at 35 ± 2 ℃ and cultivate 3-4 week down, dish is inverted in the autoclaving bag of ventilation, to reduce the evaporation of moisture and the drying of dish as much as possible in cultivating for a long time.Number goes out the number of mycobacterium bovis mutation BCG bacterium colony, and multiply by the suitable dilution coefficient to determine colony forming unit (CFU) number in each moment (S) reaction is burnt.
By diluting Cidex  solution, at 25 ± 1 ℃ of killing effects of using the glutaraldehyde of open-assembly time 5,10,20 and 30 minutes test 1.5% to mycobacterium bovis mutation BCG down with identical as mentioned above mode.Repeat whole test.
Neutralization is confirmed
1.0ml joined among the 9.0ml and recovery media in make the serial dilutions of the disinfectant of two kinds of test intensity.The mycobacterium bovis mutation BCG that all adds 200CFU in the test tube of each dilution is in the recovery meat soup of 1.0ml.At ambient temperature after 10 minutes, be that 0.45 micron film filter filters dilution with the filter opening size, and wash with the SDIW about 50ml.Filter is placed on the M7H9 agar of accompanying the Ti Shi culture plate.
In order to compare number, the mycobacterium bovis mutation BCG of the about 200CFU of adding in two pipes and in the recovery media.After following 10 minutes of the environmental temperature, be that 0.45 micron film filter filters dilution with the filter opening size, and wash with the SDIW about 50ml.Filter is placed on the M7H9 agar of accompanying the Ti Shi culture plate.
To coil the autoclaving bag that under 35 ± 2 ℃, places ventilation and cultivate 3-4 week.All there is similar number to confirm that disinfectant and phenol neutralize by recovery process on all dishes.
The original mycobacterium bovis mutation BCG number (S of affirmation in reaction flask 0)
Test cultures is through checking to determine the number of the initial CFU in reaction flask.In the SDIW of 45ml, add 5.0ml mycobacterium bovis mutation BCG, make solution become the whirlpool shape to mix.Take out 1.0ml, in 9ml He in the recovery media, make ten times of continuous dilutions.Make 3 groups of dilutions.Dilution 3-6 is that 0.45 micron film filter filters with the filter opening size, and washes with the SDIW about 50ml.Filtrate is placed on the M7H9 agar of accompanying the Ti Shi culture plate, and under 35 ± 2 ℃, place the autoclaving bag of ventilation to cultivate 4-5 week.Number goes out the number of mycobacterium bovis mutation BCG bacterium colony, and multiply by the suitable dilution coefficient to determine the number (S of initial CFU in reaction flask 0).
In above-mentioned two researchs, the undiluted recipe ratio that contains the potassium acetate of 2.4% glutaraldehyde, 15% isopropyl alcohol and 5% of the present invention comprises that other prescriptions of Cidex  can kill mycobacterium bovis mutation BCG quickly.To containing 1.6% the glutaraldehyde of having an appointment, 10% isopropyl alcohol and 3.33% potassium acetate, the prescription of this dilution occupies second on the speed of killing mycobacterium bovis mutation BCG with 1.5 times of same recipe dilutions.The prescription of isopropyl alcohol (no potassium acetate) that contains 2.4% glutaraldehyde and 15% on the speed of killing mycobacterium bovis mutation BCG slightly faster than the prescription and the Cidex  that contain 2.4% and 5% potassium acetate (no isopropanol).
Embodiment 3
Present embodiment shows various glutaraldehydes (GA) test solution is carried out the glutaraldehyde test that (steam) measures relative concentration in air.
With volume be 1.0L 1) be 1.0 in water, 2.0, with 2.5% GA, 2) be 1.0 in water, 2.0 and 2.5% GA adds 25% IPA, 3) glutaraldehyde solution (2.5% GA) of Cidex  activation, 4) the present invention (3.0% GA+25% IPA+8% acetate) and 5) formula I V (2.5% GA+20% IPA+8% acetate) places at the bottom of 5.0 gallons the glass jar.Clog jar, stopper has the glass tube of two kinds of different sizes, and long glass tube is connected to air pump, and short glass tube is connected to the gas stone in MBTH solution.After the saturation of the air reaches 1.0 hours, the air that headroom by glass jar pumps into constant volume to 0.5% 3-methyl-2-and the thiazolone (MBTH) of 50.0ml, kept 5.0 minutes, 1.75% the oxidant (adding Iron(III) chloride hexahydrate and sulfamic acid) that adds 20.0ml, kept 1.0 hours, and measured the color that is produced and judge trap.GA and these solution reactions are transformed into the green of the various intensity that the concentration with GA has functional relation.Measurement result directly with the calibration curve contrast, with definite GA that discharges by the various test solutions of 1.0L concentration of (steam) in air.The result demonstrates, and when having acetate, steam is inhibited.
Significantly be lower than the steam that Cidex  solution discharges from the glutaraldehyde steam that discharges the prescription of pure and mild acetate that contains of the present invention always.For example, be that the prescription of potassium acetate of 3% glutaraldehyde, 25% isopropyl alcohol and 8% and demarcation are that the glutaraldehyde steam that the prescription of the potassium acetate of 2.5% glutaraldehyde, 20% isopropyl alcohol and 8% discharges lacks 65-80% and 78-85% than Cidex  (2.5% glutaraldehyde) respectively by demarcation.Therefore, studies have shown that originally glutaraldehyde steam that the present invention's (glutaraldehyde+isopropyl alcohol+acetate) discharges is far fewer than discharging from Cidex  (only glutaraldehyde).The level of the glutaraldehyde steam that the contrast glutaraldehyde+isopropyl alcohol that discharges from glutaraldehyde discharges is also similar.Conclusion is that acetate is to a certain degree suppressing the glutaraldehyde steam down.
Above data and clearly illustrate that by the conclusion that these data obtain the present invention has realized the main purpose of all propositions at least.

Claims (9)

  1. One kind at room temperature smell is little, and the pH with activation is the water miscible disinfectant and/or the water base solution of sterilizing of about 7.3-about 7.9, it comprises: the glutaraldehyde of the about 2.0%-of percent by volume about 5.0%, the alcohol of the about 5.0%-of percent by volume about 26.0%, wherein, alcohol is selected from the group of being made up of methyl alcohol, ethanol and isopropyl alcohol; The buffer compatible of effective dose with glutaraldehyde; And the acetate of the about 3%-of percentage by weight about 8%.
  2. 2. the water miscible disinfectant and/or the antiseptic solution of claim 1, wherein, the concentration of alcohol is percent by volume 24%-about 26%.
  3. 3. the water miscible disinfectant and/or the antiseptic solution of claim 1, wherein, buffer is a phosphate buffer.
  4. 4. the water miscible disinfectant and/or the antiseptic solution of claim 1 further comprise the surfactant of percentage by weight 0.0025%-0.01%, and it is selected from the group of being made up of nonionic, cation and anionic surfactant.
  5. 5. the water miscible disinfectant and/or the antiseptic solution of claim 4, wherein, surfactant is the surfactant of nonionic.
  6. 6. a bactericidal properties that improves the glutaraldehyde disinfectant group water solution and reduce the method for its smell comprises: be used in combination concentration and be the low-alcohol solution of straight or branched of the about 5%-of percent by volume about 26% and the acetate of percentage by weight 3%-8%.
  7. 7. disinfectant kit, comprise: but first container of storage solutions deposited, but wherein can stablize the glutaraldehyde that the storage solutions that reaches 12 months contains the about 2%-of percent by volume about 5%, the alcohol of the about 5%-of percent by volume about 26%, wherein, alcohol is selected from the group of being made up of methyl alcohol, ethanol and isopropyl alcohol, and the acetate of the about 3%-of percentage by weight about 8%; Deposit second container of phosphate buffer, the amount of buffer is enough to provide 4g/l whenever to be raised to the concentration of every liter of 7g/l, and the pH that provides when merging with first container is 7.3-7.9; And mix described first and second containers so that the specification that can stablize the antiseptic solution that reaches 14 days to be provided.
  8. 8. the water miscible disinfectant and/or the antiseptic solution of claim 2, wherein, the percentage by weight of acetate is about 8%.
  9. 9. disinfectant kit, comprise: but first container of storage solutions deposited, but wherein can stablize the glutaraldehyde that the storage solutions that reaches 12 months contains the about 2%-of percent by volume about 5%, the alcohol of the about 24%-of percent by volume about 26%, wherein, alcohol is selected from the group of being made up of methyl alcohol, ethanol and isopropyl alcohol, and the acetate of percentage by weight about 8%; Deposit second container of phosphate buffer, the amount of buffer is enough to provide 4g/l whenever to be raised to the concentration of every liter of 7g/l, and the pH that provides when merging with first container is 7.3-7.9; And mix described first and second containers so that the specification that can stablize the antiseptic solution that reaches 14 days to be provided.
CN200680025411XA 2005-07-11 2006-06-27 Enhanced tuburculocidal activity and decreased fumes from glutaraldehyde disinfectant using acetate salts and alcohol Active CN101222846B (en)

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