CN101213300B - 用于rna干扰的寡核苷酸及其生物学应用 - Google Patents

用于rna干扰的寡核苷酸及其生物学应用 Download PDF

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CN101213300B
CN101213300B CN2006800237824A CN200680023782A CN101213300B CN 101213300 B CN101213300 B CN 101213300B CN 2006800237824 A CN2006800237824 A CN 2006800237824A CN 200680023782 A CN200680023782 A CN 200680023782A CN 101213300 B CN101213300 B CN 101213300B
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oligonucleotide
sequence
nucleotide
chain
transfection
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CN101213300A (zh
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J-P·拜赫
A-L·波尔卡托贝勒敏
P·厄巴彻
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Polyplus Transfection SA
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Polyplus Transfection SA
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
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    • A61K31/713—Double-stranded nucleic acids or oligonucleotides
    • A—HUMAN NECESSITIES
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    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/34—Macromolecular compounds obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds, e.g. polyesters, polyamino acids, polysiloxanes, polyphosphazines, copolymers of polyalkylene glycol or poloxamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
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    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/52—Methods for regulating/modulating their activity modulating the physical stability, e.g. GC-content

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
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  • Proteomics, Peptides & Aminoacids (AREA)
  • Inorganic Chemistry (AREA)
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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
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CN2006800237824A 2005-06-01 2006-06-01 用于rna干扰的寡核苷酸及其生物学应用 Active CN101213300B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US68601005P 2005-06-01 2005-06-01
US60/686,010 2005-06-01
PCT/EP2006/006340 WO2006128739A1 (en) 2005-06-01 2006-06-01 Oligonucleotides for rna interference and biological applications thereof

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CN101213300A CN101213300A (zh) 2008-07-02
CN101213300B true CN101213300B (zh) 2013-01-23

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US (2) US8802640B2 (enExample)
EP (1) EP1888749B1 (enExample)
JP (1) JP5371424B2 (enExample)
CN (1) CN101213300B (enExample)
DK (1) DK1888749T3 (enExample)
WO (1) WO2006128739A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8802640B2 (en) 2005-06-01 2014-08-12 Polyplus-Transfection Sa Oligonucleotides for RNA interference and biological applications thereof
DK2056845T3 (da) * 2006-08-08 2017-11-27 Rheinische Friedrich-Wilhelms-Universität Bonn Struktur og anvendelse af 5'-phosphat-oligonukleotider
EP2146691A2 (en) 2007-04-17 2010-01-27 Baxter International Inc. Nucleic acid microparticles for pulmonary delivery
US9738680B2 (en) 2008-05-21 2017-08-22 Rheinische Friedrich-Wilhelms-Universität Bonn 5′ triphosphate oligonucleotide with blunt end and uses thereof
EP2508530A1 (en) 2011-03-28 2012-10-10 Rheinische Friedrich-Wilhelms-Universität Bonn Purification of triphosphorylated oligonucleotides using capture tags
EP2712870A1 (en) 2012-09-27 2014-04-02 Rheinische Friedrich-Wilhelms-Universität Bonn Novel RIG-I ligands and methods for producing them
US20170137808A1 (en) * 2014-03-20 2017-05-18 Oommen Varghese Improved small interfering ribonucleic acid molecules
WO2016110691A1 (en) * 2015-01-06 2016-07-14 The University Court Of The University Of Aberdeen Enhanced rnai mediated gene regulation

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1568373A (zh) * 2000-12-01 2005-01-19 马普科技促进协会 介导rna干涉的小rna分子

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6506559B1 (en) * 1997-12-23 2003-01-14 Carnegie Institute Of Washington Genetic inhibition by double-stranded RNA
WO2002081628A2 (en) * 2001-04-05 2002-10-17 Ribozyme Pharmaceuticals, Incorporated Modulation of gene expression associated with inflammation proliferation and neurite outgrowth, using nucleic acid based technologies
US20030212022A1 (en) * 2001-03-23 2003-11-13 Jean-Marie Vogel Compositions and methods for gene therapy
DK2796553T3 (da) 2000-03-30 2019-09-30 Whitehead Inst Biomedical Res Rna-sekvensspecifikke formidlere af rna-interferens
US8802640B2 (en) 2005-06-01 2014-08-12 Polyplus-Transfection Sa Oligonucleotides for RNA interference and biological applications thereof

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1568373A (zh) * 2000-12-01 2005-01-19 马普科技促进协会 介导rna干涉的小rna分子

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Makoto Miyagishi and Kazunari Taira.U6 promoter-driven siRNAs with four uridine 3"overhangs efficiently suppress targeted gene expression in mammalian cells.Nat Biotechnol.2002,20497-500. *

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EP1888749A1 (en) 2008-02-20
US9243248B2 (en) 2016-01-26
US20080153772A1 (en) 2008-06-26
JP5371424B2 (ja) 2013-12-18
US20140343125A1 (en) 2014-11-20
JP2008541745A (ja) 2008-11-27
EP1888749B1 (en) 2014-10-15
US8802640B2 (en) 2014-08-12
CN101213300A (zh) 2008-07-02
DK1888749T3 (en) 2015-01-05
WO2006128739A1 (en) 2006-12-07

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