CN101210879B - Device for conveniently replacing surface plasma resonance biochip and its usage method - Google Patents
Device for conveniently replacing surface plasma resonance biochip and its usage method Download PDFInfo
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- CN101210879B CN101210879B CN 200610128490 CN200610128490A CN101210879B CN 101210879 B CN101210879 B CN 101210879B CN 200610128490 CN200610128490 CN 200610128490 CN 200610128490 A CN200610128490 A CN 200610128490A CN 101210879 B CN101210879 B CN 101210879B
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Abstract
The invention discloses a device for conveniently replacing a surface plasma resonance biochip and an application method thereof. The device comprises a biochip body, a biochip protective sleeve, a biochip lead-in mechanism, a biochip positioning device and a biochip loading mechanism. The application method comprises the following steps of: pressing a biochip loading button, pressing the biochip protective sleeve to take out a biochip together with the protective sleeve; and loading the biochip by leading-in the biochip together with the protective sleeve through the biochip lead-in mechanism and automatically separating the biochip from the biochip protective sleeve to expose the sensitive part of the biochip. The invention is helpful for replacing surface plasma resonance biochip, improves the application convenience of surface plasma resonance bioanalyzer, simplifies replacement procedure, shortens bioanalysis time, saves bioanalysis cost, and is suitable for batch production.
Description
Technical field
The present invention relates to a kind of replacing surface plasma resonance biochip technology and device, relate to a kind of device for conveniently replacing surface plasma resonance biochip and using method specifically.
Background technology
Biology sensor develops over past ten years rapidly surface plasma resonance (SPR, other are same), is a kind of very potential novel optical biosensor technique.The mechanism of surface plasmon resonance biosensor is: the Recognition of Biomolecular film is fixed on spr sensor Au film surface, the molecular recognition film is caught tested analyte, the variations in refractive index that causes sensors A u film surface, therefore need not labeled molecule and just can directly test, realize that nondestructive real-time online detects.It is the product with the optical fiber transmission technique combination of highly sensitive surface plasma sensing technology and low-energy-consumption, can make sensitive response to the subtle change of sensor surface testing sample, be applicable to the biology and the chemical reaction of material and sample solution in the research sensor surface sensitive layer, thus the micro-biological and chemical material in the quantitative measurement sample solution.In recent years, the portability of SPR biological analyser and microminiaturization are emerging research focuses, and the portable SPR biological analyser of having developed is all based on integral replacing surface plasmon resonance biosensor technology.Surface plasmon resonance biosensor is made of cover optical detection system and a biology sensor body, biosensor surface grown Au film and the biological coupling connection film of 50nm.The 50nm gold film and the bio-sensitive film of biosensor surface are the keys of surface plasmon resonance biosensor, in actual applications, bio-sensitive film is subjected to the sensing number of times and functional deterioration can not produce correct signal response to sample, at this moment just needs to change biology sensor.Usually the biology sensor cost is more expensive, integral replacing examination must make customer using cost increase, so patent of the present invention proposes not change sensor body, only changes biologic sensor chip, and biologic sensor chip can regenerate, so have very significant meaning.
Summary of the invention
Purpose of the present invention just is to provide a kind of not integral replacing biology sensor, and only changes the device for conveniently replacing surface plasma resonance biochip and the using method of bio-sensing chip.
Purpose of the present invention can realize by following measure:
The present invention includes seal casinghousing, be provided with the circulation stall in the seal casinghousing bottom, on the circulation stall, be mounted with flow cell, in circulation stall both sides is the bracing or strutting arrangement of biochip guide groove, be mounted with the surface plasmon resonance biosensor assembly in circulation stall the place ahead, it above the surface plasmon resonance biosensor assembly biology sensor compact heap, symmetry offers the biochip guide groove respectively on the bracing or strutting arrangement of biochip guide groove, in the biochip guide groove, be mounted with the support membrane flat spring of biochip, place, bracing or strutting arrangement both sides at the biochip guide groove is provided with the bio-sensing lift cam respectively, it on the bio-sensing lift cam cam back up pad, the cam back up pad is connected on the surface plasmon resonance biosensor assembly, on the bio-sensing lift cam is coaxial, also be provided with biochip loading attachment gear, on biochip loading attachment gear, be mounted with biochip loading attachment tooth bar, be connected with biology sensor on the biochip loading attachment tooth bar and load button;
In the biochip guide groove, settle bio-sensing chip assembling mounted mechanism, described bio-sensing chip assembling mounted mechanism comprises the biochip protection sleeve, on the biochip protection sleeve, offer chute, in chute, be connected with biochip body push rod, the bottom of push rod is slidably connected on the housing, between biochip body push rod and housing, also be provided with retracing spring, be provided with push rod in biochip protection sleeve one side, push rod extends to outside the housing by the hole on the housing, and the end outside push rod is positioned at housing is mounted with biochip and loads button;
Be provided with the biochip detent mechanism in surface plasmon resonance biosensor assembly bottom, this detent mechanism comprises the stationary installation that is connected the biology sensor on the surface plasmon resonance biosensor assembly, is provided with biochip register pin and register pin retracing spring in the stationary installation of biology sensor.
The present invention's seal casinghousing is provided with locator slide, is mounted with the biology sensor compact heap in locator slide.Perhaps, seal casinghousing is provided with pull bar, is mounted with the biology sensor compact heap at the pull bar end.Offer axis hole on the cam back up pad, the surface plasmon resonance biosensor assembly is provided with axle, and the cam back up pad is connected with the surface plasmon resonance biosensor assembly by above-mentioned axis hole and axle.Biology sensor loads button and is connected with biochip loading attachment tooth bar by push rod.Be connected with biochip body push rod in chute, the bottom of biochip body push rod is connected on the housing by chute.Perhaps, be connected with biochip body push rod in chute, the bottom of biochip body push rod is connected on the housing by register pin.Front end at push rod is provided with and the contacted push rod roller of biochip body push rod.
The using method of device for conveniently replacing surface plasma resonance biochip of the present invention is as follows:
When the surface plasma resonance biological group is downloaded, press biology sensor earlier and load button, the surface plasmon resonance biosensor assembly moves up and abdicates the space of 2-5mm, promote the surface plasma resonance biological group then, biochip takes out with the biochip protection sleeve in the surface plasma resonance biological group, discharge biology sensor again and load button, finish the unloading of surface plasma resonance biological group; When the surface plasma resonance biological assembling is carried, press biology sensor earlier and load button, the surface plasmon resonance biosensor assembly moves up and abdicates the space of 2-5mm, the surface plasma resonance biological group is imported by bio-sensing chip assembling mounted mechanism, automatically biochip in the surface plasma resonance biological group is separated with the biochip protection sleeve, abdicate the sensitive area in the surface plasma resonance biological group, discharge biology sensor again and load button, finish the surface plasma resonance biological assembling and carry;
Wherein, loading to the surface plasma resonance biological group is as follows: push biochip and load button, biochip body push rod promotes biochip by the push rod roller and moves forward, the bio-sensing face of biochip just in time is in biology sensor optical detection position, discharge biology sensor and load button, the biochip register pin of surface plasmon resonance biosensor assembly is arranged in pilot hole, finishes the location of biochip; Discharge biology sensor and load button and biochip loading button, the biochip register pin is under the effect of register pin retracing spring, and the biochip register pin leaves pilot hole, finishes biochip and separates with the biology sensor body; Retracing spring will make biochip place the biochip protection sleeve then, finish the protection of biochip protection sleeve to biochip.
The present invention is owing to adopt said structure and method, and the integral replacing biology sensor is not only changed bio-sensing chip, greatly reduces user's use cost, and the suitable batch process of this device, has great economic benefit.
Description of drawings
Fig. 1-the 1st, front view of the present invention;
Fig. 1-2 is the left view of Fig. 1 of the present invention;
Fig. 2 is the structural representation that bio-sensing chip assembling of the present invention is carried;
Fig. 3 is the structural representation of the detent mechanism of biochip of the present invention.
Embodiment
The present invention does with detailed description below in conjunction with drawings and Examples:
Embodiment 1
Device for conveniently replacing surface plasma resonance biochip of the present invention comprises seal casinghousing (1), be provided with circulation stall (11) in seal casinghousing (1) bottom, on circulation stall (11), be mounted with flow cell (10), in circulation stall (11) both sides is the bracing or strutting arrangement (12) of biochip guide groove, be mounted with surface plasmon resonance biosensor assembly (3) in circulation stall (11) the place ahead, in surface plasmon resonance biosensor assembly (3) top is biology sensor compact heap (2), symmetry offers biochip guide groove (8) respectively on the bracing or strutting arrangement (12) of biochip guide groove, in biochip guide groove (8), be mounted with the support membrane flat spring (13) of biochip, place, bracing or strutting arrangement (12) both sides at the biochip guide groove is provided with bio-sensing lift cam (7) respectively, going up in bio-sensing lift cam (7) is cam back up pad (4), cam back up pad (4) is connected on the surface plasmon resonance biosensor assembly (3), on bio-sensing lift cam (7) is coaxial, also be provided with on the biochip loading attachment gear (6), be mounted with biochip loading attachment tooth bar (5) at biochip loading attachment gear (6), be connected with biology sensor on the biochip loading attachment tooth bar (5) and load button (14);
In biochip guide groove (8), settle bio-sensing chip assembling mounted mechanism, described bio-sensing chip assembling mounted mechanism comprises biochip protection sleeve (24), on biochip protection sleeve (24), offer chute, in chute, be connected with biochip body push rod (15), the bottom of push rod is slidably connected on the housing (1), between biochip body push rod (15) and housing (1), also be provided with retracing spring (19), be provided with push rod (18) in biochip protection sleeve (24) one sides, push rod (18) extends to outside the housing by the hole on the housing (1), and the end outside push rod is positioned at housing is mounted with biochip and loads button (20);
Be provided with the biochip detent mechanism in surface plasmon resonance biosensor assembly (3) bottom, this detent mechanism comprises the stationary installation (27) that is connected the biology sensor on the surface plasmon resonance biosensor assembly (3), is provided with biochip register pin (25) and register pin retracing spring (26) in the stationary installation (27) of biology sensor.
The using method of device for conveniently replacing surface plasma resonance biochip of the present invention is as follows:
When surface plasma resonance biological group (9) is downloaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 2mm, promote surface plasma resonance biological group (9) then, biochip (23) takes out with biochip protection sleeve (24) in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish the unloading of surface plasma resonance biological group (9); When surface plasma resonance biological group (9) is loaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 2mm, surface plasma resonance biological group (9) is imported by bio-sensing chip assembling mounted mechanism, automatically biochip (23) in the surface plasma resonance biological group (9) is separated with biochip protection sleeve (24), abdicate the sensitive area in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish surface plasma resonance biological group (9) and load;
Wherein, loading to surface plasma resonance biological group (9) is as follows: push biochip and load button (20), biochip body push rod (15) promotes biochip (23) by push rod roller (17) and moves forward, the bio-sensing face (22) of biochip (23) just in time is in biology sensor optical detection position, discharge biology sensor and load button (14), the biochip register pin (25) of surface plasmon resonance biosensor assembly (3) is arranged in pilot hole (21), finishes the location of biochip (23); Discharge biology sensor and load button (14) and biochip loading button (20), biochip register pin (25) is under the effect of register pin retracing spring (26), biochip register pin (25) leaves pilot hole (21), finishes biochip (23) and separates with the biology sensor body; Retracing spring (19) will make biochip (23) place biochip protection sleeve (24) then, finish the protection of biochip protection sleeve (24) to biochip (23).
Device for conveniently replacing surface plasma resonance biochip of the present invention comprises seal casinghousing (1), be provided with circulation stall (11) in seal casinghousing (1) bottom, on circulation stall (11), be mounted with flow cell (10), in circulation stall (11) both sides is the bracing or strutting arrangement (12) of biochip guide groove, be mounted with surface plasmon resonance biosensor assembly (3) in circulation stall (11) the place ahead, in surface plasmon resonance biosensor assembly (3) top is biology sensor compact heap (2), symmetry offers biochip guide groove (8) respectively on the bracing or strutting arrangement (12) of biochip guide groove, in biochip guide groove (8), be mounted with the support membrane flat spring (13) of biochip, place, bracing or strutting arrangement (12) both sides at the biochip guide groove is provided with bio-sensing lift cam (7) respectively, going up in bio-sensing lift cam (7) is cam back up pad (4), cam back up pad (4) is connected on the surface plasmon resonance biosensor assembly (3), on bio-sensing lift cam (7) is coaxial, also be provided with on the biochip loading attachment gear (6), be mounted with biochip loading attachment tooth bar (5) at biochip loading attachment gear (6), be connected with biology sensor on the biochip loading attachment tooth bar (5) and load button (14);
In biochip guide groove (8), settle bio-sensing chip assembling mounted mechanism, described bio-sensing chip assembling mounted mechanism comprises biochip protection sleeve (24), on biochip protection sleeve (24), offer chute, in chute, be connected with biochip body push rod (15), the bottom of push rod is slidably connected on the housing (1), between biochip body push rod (15) and housing (1), also be provided with retracing spring (19), be provided with push rod (18) in biochip protection sleeve (24) one sides, push rod (18) extends to outside the housing by the hole on the housing (1), and the end outside push rod is positioned at housing is mounted with biochip and loads button (20);
Be provided with the biochip detent mechanism in surface plasmon resonance biosensor assembly (3) bottom, this detent mechanism comprises the stationary installation (27) that is connected the biology sensor on the surface plasmon resonance biosensor assembly (3), is provided with biochip register pin (25) and register pin retracing spring (26) in the stationary installation (27) of biology sensor.
Seal casinghousing (1) is provided with locator slide, is mounted with biology sensor compact heap (2) in locator slide.Perhaps, seal casinghousing (1) is provided with pull bar, is mounted with biology sensor compact heap (2) at the pull bar end.
The using method of device for conveniently replacing surface plasma resonance biochip of the present invention is as follows:
When surface plasma resonance biological group (9) is downloaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 3mm, promote surface plasma resonance biological group (9) then, biochip (23) takes out with biochip protection sleeve (24) in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish the unloading of surface plasma resonance biological group (9); When surface plasma resonance biological group (9) is loaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 3mm, surface plasma resonance biological group (9) is imported by bio-sensing chip assembling mounted mechanism, automatically biochip (23) in the surface plasma resonance biological group (9) is separated with biochip protection sleeve (24), abdicate the sensitive area in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish surface plasma resonance biological group (9) and load;
Wherein, loading to surface plasma resonance biological group (9) is as follows: push biochip and load button (20), biochip body push rod (15) promotes biochip (23) by push rod roller (17) and moves forward, the bio-sensing face (22) of biochip (23) just in time is in biology sensor optical detection position, discharge biology sensor and load button (14), the biochip register pin (25) of surface plasmon resonance biosensor assembly (3) is arranged in pilot hole (21), finishes the location of biochip (23); Discharge biology sensor and load button (14) and biochip loading button (20), biochip register pin (25) is under the effect of register pin retracing spring (26), biochip register pin (25) leaves pilot hole (21), finishes biochip (23) and separates with the biology sensor body; Retracing spring (19) will make biochip (23) place biochip protection sleeve (24) then, finish the protection of biochip protection sleeve (24) to biochip (23).
Device for conveniently replacing surface plasma resonance biochip of the present invention comprises seal casinghousing (1), be provided with circulation stall (11) in seal casinghousing (1) bottom, on circulation stall (11), be mounted with flow cell (10), in circulation stall (11) both sides is the bracing or strutting arrangement (12) of biochip guide groove, be mounted with surface plasmon resonance biosensor assembly (3) in circulation stall (11) the place ahead, in surface plasmon resonance biosensor assembly (3) top is biology sensor compact heap (2), symmetry offers biochip guide groove (8) respectively on the bracing or strutting arrangement (12) of biochip guide groove, in biochip guide groove (8), be mounted with the support membrane flat spring (13) of biochip, place, bracing or strutting arrangement (12) both sides at the biochip guide groove is provided with bio-sensing lift cam (7) respectively, going up in bio-sensing lift cam (7) is cam back up pad (4), cam back up pad (4) is connected on the surface plasmon resonance biosensor assembly (3), on bio-sensing lift cam (7) is coaxial, also be provided with on the biochip loading attachment gear (6), be mounted with biochip loading attachment tooth bar (5) at biochip loading attachment gear (6), be connected with biology sensor on the biochip loading attachment tooth bar (5) and load button (14);
In biochip guide groove (8), settle bio-sensing chip assembling mounted mechanism, described bio-sensing chip assembling mounted mechanism comprises biochip protection sleeve (24), on biochip protection sleeve (24), offer chute, in chute, be connected with biochip body push rod (15), the bottom of push rod is slidably connected on the housing (1), between biochip body push rod (15) and housing (1), also be provided with retracing spring (19), be provided with push rod (18) in biochip protection sleeve (24) one sides, push rod (18) extends to outside the housing by the hole on the housing (1), and the end outside push rod is positioned at housing is mounted with biochip and loads button (20);
Be provided with the biochip detent mechanism in surface plasmon resonance biosensor assembly (3) bottom, this detent mechanism comprises the stationary installation (27) that is connected the biology sensor on the surface plasmon resonance biosensor assembly (3), is provided with biochip register pin (25) and register pin retracing spring (26) in the stationary installation (27) of biology sensor.
Seal casinghousing (1) is provided with locator slide, is mounted with biology sensor compact heap (2) in locator slide.Perhaps, seal casinghousing (1) is provided with pull bar, is mounted with biology sensor compact heap (2) at the pull bar end.Offer axis hole on cam back up pad (4), surface plasmon resonance biosensor assembly (3) is provided with axle, and cam back up pad (4) is connected with surface plasmon resonance biosensor assembly (3) by above-mentioned axis hole and axle.
The using method of device for conveniently replacing surface plasma resonance biochip of the present invention is as follows:
When surface plasma resonance biological group (9) is downloaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 4mm, promote surface plasma resonance biological group (9) then, biochip (23) takes out with biochip protection sleeve (24) in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish the unloading of surface plasma resonance biological group (9); When surface plasma resonance biological group (9) is loaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 4mm, surface plasma resonance biological group (9) is imported by bio-sensing chip assembling mounted mechanism, automatically biochip (23) in the surface plasma resonance biological group (9) is separated with biochip protection sleeve (24), abdicate the sensitive area in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish surface plasma resonance biological group (9) and load;
Wherein, loading to surface plasma resonance biological group (9) is as follows: push biochip and load button (20), biochip body push rod (15) promotes biochip (23) by push rod roller (17) and moves forward, the bio-sensing face (22) of biochip (23) just in time is in biology sensor optical detection position, discharge biology sensor and load button (14), the biochip register pin (25) of surface plasmon resonance biosensor assembly (3) is arranged in pilot hole (21), finishes the location of biochip (23); Discharge biology sensor and load button (14) and biochip loading button (20), biochip register pin (25) is under the effect of register pin retracing spring (26), biochip register pin (25) leaves pilot hole (21), finishes biochip (23) and separates with the biology sensor body; Retracing spring (19) will make biochip (23) place biochip protection sleeve (24) then, finish the protection of biochip protection sleeve (24) to biochip (23).
Embodiment 4
Device for conveniently replacing surface plasma resonance biochip of the present invention comprises seal casinghousing (1), be provided with circulation stall (11) in seal casinghousing (1) bottom, on circulation stall (11), be mounted with flow cell (10), in circulation stall (11) both sides is the bracing or strutting arrangement (12) of biochip guide groove, be mounted with surface plasmon resonance biosensor assembly (3) in circulation stall (11) the place ahead, in surface plasmon resonance biosensor assembly (3) top is biology sensor compact heap (2), symmetry offers biochip guide groove (8) respectively on the bracing or strutting arrangement (12) of biochip guide groove, in biochip guide groove (8), be mounted with the support membrane flat spring (13) of biochip, place, bracing or strutting arrangement (12) both sides at the biochip guide groove is provided with bio-sensing lift cam (7) respectively, going up in bio-sensing lift cam (7) is cam back up pad (4), cam back up pad (4) is connected on the surface plasmon resonance biosensor assembly (3), on bio-sensing lift cam (7) is coaxial, also be provided with on the biochip loading attachment gear (6), be mounted with biochip loading attachment tooth bar (5) at biochip loading attachment gear (6), be connected with biology sensor on the biochip loading attachment tooth bar (5) and load button (14);
In biochip guide groove (8), settle bio-sensing chip assembling mounted mechanism, described bio-sensing chip assembling mounted mechanism comprises biochip protection sleeve (24), on biochip protection sleeve (24), offer chute, in chute, be connected with biochip body push rod (15), the bottom of push rod is slidably connected on the housing (1), between biochip body push rod (15) and housing (1), also be provided with retracing spring (19), be provided with push rod (18) in biochip protection sleeve (24) one sides, push rod (18) extends to outside the housing by the hole on the housing (1), and the end outside push rod is positioned at housing is mounted with biochip and loads button (20);
Be provided with the biochip detent mechanism in surface plasmon resonance biosensor assembly (3) bottom, this detent mechanism comprises the stationary installation (27) that is connected the biology sensor on the surface plasmon resonance biosensor assembly (3), is provided with biochip register pin (25) and register pin retracing spring (26) in the stationary installation (27) of biology sensor.
Seal casinghousing (1) is provided with locator slide, is mounted with biology sensor compact heap (2) in locator slide.Perhaps, seal casinghousing (1) is provided with pull bar, is mounted with biology sensor compact heap (2) at the pull bar end.Offer axis hole on cam back up pad (4), surface plasmon resonance biosensor assembly (3) is provided with axle, and cam back up pad (4) is connected with surface plasmon resonance biosensor assembly (3) by above-mentioned axis hole and axle.Biology sensor loads button (14) and is connected with biochip loading attachment tooth bar (5) by push rod.Be connected with biochip body push rod (15) in chute, the bottom of biochip body push rod is connected on the housing by chute.
The using method of device for conveniently replacing surface plasma resonance biochip of the present invention is as follows:
When surface plasma resonance biological group (9) is downloaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 4.5mm, promote surface plasma resonance biological group (9) then, biochip (23) takes out with biochip protection sleeve (24) in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish the unloading of surface plasma resonance biological group (9); When surface plasma resonance biological group (9) is loaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 4.5mm, surface plasma resonance biological group (9) is imported by bio-sensing chip assembling mounted mechanism, automatically biochip (23) in the surface plasma resonance biological group (9) is separated with biochip protection sleeve (24), abdicate the sensitive area in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish surface plasma resonance biological group (9) and load;
Wherein, loading to surface plasma resonance biological group (9) is as follows: push biochip and load button (20), biochip body push rod (15) promotes biochip (23) by push rod roller (17) and moves forward, the bio-sensing face (22) of biochip (23) just in time is in biology sensor optical detection position, discharge biology sensor and load button (14), the biochip register pin (25) of surface plasmon resonance biosensor assembly (3) is arranged in pilot hole (21), finishes the location of biochip (23); Discharge biology sensor and load button (14) and biochip loading button (20), biochip register pin (25) is under the effect of register pin retracing spring (26), biochip register pin (25) leaves pilot hole (21), finishes biochip (23) and separates with the biology sensor body; Retracing spring (19) will make biochip (23) place biochip protection sleeve (24) then, finish the protection of biochip protection sleeve (24) to biochip (23).
Device for conveniently replacing surface plasma resonance biochip of the present invention comprises seal casinghousing (1), be provided with circulation stall (11) in seal casinghousing (1) bottom, on circulation stall (11), be mounted with flow cell (10), in circulation stall (11) both sides is the bracing or strutting arrangement (12) of biochip guide groove, be mounted with surface plasmon resonance biosensor assembly (3) in circulation stall (11) the place ahead, in surface plasmon resonance biosensor assembly (3) top is biology sensor compact heap (2), symmetry offers biochip guide groove (8) respectively on the bracing or strutting arrangement (12) of biochip guide groove, in biochip guide groove (8), be mounted with the support membrane flat spring (13) of biochip, place, bracing or strutting arrangement (12) both sides at the biochip guide groove is provided with bio-sensing lift cam (7) respectively, going up in bio-sensing lift cam (7) is cam back up pad (4), cam back up pad (4) is connected on the surface plasmon resonance biosensor assembly (3), on bio-sensing lift cam (7) is coaxial, also be provided with on the biochip loading attachment gear (6), be mounted with biochip loading attachment tooth bar (5) at biochip loading attachment gear (6), be connected with biology sensor on the biochip loading attachment tooth bar (5) and load button (14);
In biochip guide groove (8), settle bio-sensing chip assembling mounted mechanism, described bio-sensing chip assembling mounted mechanism comprises biochip protection sleeve (24), on biochip protection sleeve (24), offer chute, in chute, be connected with biochip body push rod (15), the bottom of push rod is slidably connected on the housing (1), between biochip body push rod (15) and housing (1), also be provided with retracing spring (19), be provided with push rod (18) in biochip protection sleeve (24) one sides, push rod (18) extends to outside the housing by the hole on the housing (1), and the end outside push rod is positioned at housing is mounted with biochip and loads button (20);
Be provided with the biochip detent mechanism in surface plasmon resonance biosensor assembly (3) bottom, this detent mechanism comprises the stationary installation (27) that is connected the biology sensor on the surface plasmon resonance biosensor assembly (3), is provided with biochip register pin (25) and register pin retracing spring (26) in the stationary installation (27) of biology sensor.
Seal casinghousing (1) is provided with locator slide, is mounted with biology sensor compact heap (2) in locator slide.Perhaps, seal casinghousing (1) is provided with pull bar, is mounted with biology sensor compact heap (2) at the pull bar end.Offer axis hole on cam back up pad (4), surface plasmon resonance biosensor assembly (3) is provided with axle, and cam back up pad (4) is connected with surface plasmon resonance biosensor assembly (3) by above-mentioned axis hole and axle.Biology sensor loads button (14) and is connected with biochip loading attachment tooth bar (5) by push rod.Be connected with biochip body push rod (15) in chute, the bottom of biochip body push rod is connected on the housing by chute.Perhaps, be connected with biochip body push rod (15) in chute, the bottom of biochip body push rod is connected on the housing by register pin (16).Front end at push rod (18) is provided with and the contacted push rod roller of biochip body push rod (15) (17).
The using method of the prompt replacing of the present invention surface plasma resonance biochip device is as follows:
The technical solution adopted in the present invention is:
1. the golden film of bio-sensing chip superficial growth 50nm is discerned film with the biomolecule that is used for responsive measured matter;
2. biologic sensor chip places in the protection sleeve, and the protection biochip is not corroded by extraneous objectionable impurities and pollutes;
3. press the loading button, the biochip group that will have protection sleeve is inserted together along guide rail, protection sleeve is abdicated biochip automatically, and makes biochip place the sensor sensing position by locating device, and biochip is in the duty of sensor under hold down gag;
4, take out biochip if desired, as long as click the loading button, this moment, biochip separated with sensor body, advanced the protection sleeve of biochip, and the protection biochip is then with biochip and taking-up of protection sleeve.
Specifically:
When surface plasma resonance biological group (9) is downloaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 5mm, promote surface plasma resonance biological group (9) then, biochip (23) takes out with biochip protection sleeve (24) in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish the unloading of surface plasma resonance biological group (9); When surface plasma resonance biological group (9) is loaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 5mm, surface plasma resonance biological group (9) is imported by bio-sensing chip assembling mounted mechanism, automatically biochip (23) in the surface plasma resonance biological group (9) is separated with biochip protection sleeve (24), abdicate the sensitive area in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish surface plasma resonance biological group (9) and load;
Wherein, loading to surface plasma resonance biological group (9) is as follows: push biochip and load button (20), biochip body push rod (15) promotes biochip (23) by push rod roller (17) and moves forward, the bio-sensing face (22) of biochip (23) just in time is in biology sensor optical detection position, discharge biology sensor and load button (14), the biochip register pin (25) of surface plasmon resonance biosensor assembly (3) is arranged in pilot hole (21), finishes the location of biochip (23); Discharge biology sensor and load button (14) and biochip loading button (20), biochip register pin (25) is under the effect of register pin retracing spring (26), biochip register pin (25) leaves pilot hole (21), finishes biochip (23) and separates with the biology sensor body; Retracing spring (19) will make biochip (23) place biochip protection sleeve (24) then, finish the protection of biochip protection sleeve (24) to biochip (23).
Claims (9)
1. device for conveniently replacing surface plasma resonance biochip, it is characterized in that: it comprises seal casinghousing (1), be provided with circulation stall (11) in seal casinghousing (1) bottom, on circulation stall (11), be mounted with flow cell (10), in circulation stall (11) both sides is the bracing or strutting arrangement (12) of biochip guide groove, be mounted with surface plasmon resonance biosensor assembly (3) in circulation stall (11) the place ahead, in surface plasmon resonance biosensor assembly (3) top is biology sensor compact heap (2), symmetry offers biochip guide groove (8) respectively on the bracing or strutting arrangement (12) of biochip guide groove, in biochip guide groove (8), be mounted with the support membrane flat spring (13) of biochip, place, bracing or strutting arrangement (12) both sides at the biochip guide groove is provided with bio-sensing lift cam (7) respectively, going up in bio-sensing lift cam (7) is cam back up pad (4), cam back up pad (4) is connected on the surface plasmon resonance biosensor assembly (3), on bio-sensing lift cam (7) is coaxial, also be provided with biochip loading attachment gear (6), on biochip loading attachment gear (6), be mounted with biochip loading attachment tooth bar (5), be connected with biology sensor on the biochip loading attachment tooth bar (5) and load button (14);
In biochip guide groove (8), settle bio-sensing chip assembling mounted mechanism, described bio-sensing chip assembling mounted mechanism comprises biochip protection sleeve (24), on biochip protection sleeve (24), offer chute, in chute, be connected with biochip body push rod (15), the bottom of biochip body push rod is slidably connected on the housing (1), between biochip body push rod (15) and housing (1), also be provided with retracing spring (19), be provided with push rod (18) in biochip protection sleeve (24) one sides, push rod (18) extends to outside the housing by the hole on the housing (1), and the end outside push rod is positioned at housing is mounted with biochip and loads button (20);
Be provided with the biochip detent mechanism in surface plasmon resonance biosensor assembly (3) bottom, this detent mechanism comprises the stationary installation (27) that is connected the biology sensor on the surface plasmon resonance biosensor assembly (3), is provided with biochip register pin (25) and register pin retracing spring (26) in the stationary installation (27) of biology sensor.
2. the described device for conveniently replacing surface plasma resonance biochip of claim 1, it is characterized in that: described seal casinghousing (1) is provided with locator slide, is mounted with biology sensor compact heap (2) in locator slide.
3. the described device for conveniently replacing surface plasma resonance biochip of claim 1, it is characterized in that: described seal casinghousing (1) is provided with pull bar, is mounted with biology sensor compact heap (2) at the pull bar end.
4. the described device for conveniently replacing surface plasma resonance biochip of claim 1, it is characterized in that: on described cam back up pad (4), offer axis hole, surface plasmon resonance biosensor assembly (3) is provided with axle, and cam back up pad (4) is connected with surface plasmon resonance biosensor assembly (3) by above-mentioned axis hole and axle.
5. the described device for conveniently replacing surface plasma resonance biochip of claim 1 is characterized in that: described biology sensor loads button (14) and is connected with biochip loading attachment tooth bar (5) by push rod.
6. the described device for conveniently replacing surface plasma resonance biochip of claim 1, it is characterized in that: be connected with biochip body push rod (15) in described chute, the bottom of biochip body push rod is connected on the housing by chute.
7. the described device for conveniently replacing surface plasma resonance biochip of claim 1, it is characterized in that: be connected with biochip body push rod (15) in described chute, the bottom of biochip body push rod is connected on the housing by register pin (16).
8. the described device for conveniently replacing surface plasma resonance biochip of claim 1, it is characterized in that: the front end at described push rod (18) is provided with and the contacted push rod roller of biochip body push rod (15) (17).
9. using method of device for conveniently replacing surface plasma resonance biochip according to claim 1, it is characterized in that: described using method is as follows:
When surface plasma resonance biological group (9) is downloaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 2-5mm, promote surface plasma resonance biological group (9) then, biochip (23) takes out with biochip protection sleeve (24) in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish the unloading of surface plasma resonance biological group (9); When surface plasma resonance biological group (9) is loaded, press biology sensor earlier and load button (14), surface plasmon resonance biosensor assembly (3) moves up and abdicates the space of 2-5mm, surface plasma resonance biological group (9) is imported by bio-sensing chip assembling mounted mechanism, automatically biochip (23) in the surface plasma resonance biological group (9) is separated with biochip protection sleeve (24), abdicate the sensitive area in the surface plasma resonance biological group (9), discharge biology sensor again and load button (14), finish surface plasma resonance biological group (9) and load;
Wherein, carry as follows to loading of surface plasma resonance biological group (9): push biochip and load button (20), biochip body push rod (15) promotes biochip (23) by push rod roller (17) and moves forward, the bio-sensing face (22) of biochip (23) just in time is in biology sensor optical detection position, discharge biology sensor and load button (14), the biochip register pin (25) of surface plasmon resonance biosensor assembly (3) is arranged in pilot hole (21), finishes the location of biochip (23); Discharge biology sensor and load button (14) and biochip loading button (20), biochip register pin (25) is under the effect of register pin retracing spring (26), biochip register pin (25) leaves pilot hole (21), finishes biochip (23) and separates with the biology sensor body; Retracing spring (19) will make biochip (23) place biochip protection sleeve (24) then, finish the protection of biochip protection sleeve (24) to biochip (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610128490 CN101210879B (en) | 2006-12-29 | 2006-12-29 | Device for conveniently replacing surface plasma resonance biochip and its usage method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610128490 CN101210879B (en) | 2006-12-29 | 2006-12-29 | Device for conveniently replacing surface plasma resonance biochip and its usage method |
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| CN101210879A CN101210879A (en) | 2008-07-02 |
| CN101210879B true CN101210879B (en) | 2010-08-18 |
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| CN 200610128490 Expired - Fee Related CN101210879B (en) | 2006-12-29 | 2006-12-29 | Device for conveniently replacing surface plasma resonance biochip and its usage method |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101539570B (en) * | 2009-04-30 | 2013-02-06 | 河南农业大学 | Coupling device of optical SPR biochip, sensor and microfluid pool |
| CN102410989A (en) * | 2011-11-16 | 2012-04-11 | 河南农业大学 | Dual-channel MDOF (multidegree of freedom) adjustment mechanism of optical surface plasma resonance biosensor |
| CN102618435B (en) * | 2012-04-01 | 2013-07-24 | 上海百傲科技有限公司 | Adaptive sealed reaction chamber for biochip reaction device |
| CN102928344B (en) * | 2012-10-26 | 2014-12-31 | 湖北三江航天红峰控制有限公司 | Locking mechanism for photoelectric sensor |
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