CN101204613B - Method using microorganism for reducing rudimental tylosin in dregs of a decoction - Google Patents
Method using microorganism for reducing rudimental tylosin in dregs of a decoction Download PDFInfo
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- CN101204613B CN101204613B CN2007101971953A CN200710197195A CN101204613B CN 101204613 B CN101204613 B CN 101204613B CN 2007101971953 A CN2007101971953 A CN 2007101971953A CN 200710197195 A CN200710197195 A CN 200710197195A CN 101204613 B CN101204613 B CN 101204613B
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Abstract
The invention relates to a method for reducing rudimental tylosin in herb residue, particularly to a method for utilizing a microbiological method to reduce the rudimental tylosin in herb residue, which is characterized in that the method comprises the steps that: a. formulating herb residue culture medium; b. preparing bacterial suspension; c. adding Candida tropicalis and Em undiluted suspension prepared in step b to the herb residue culture medium prepared in step a, adding respectively 4 to 10 mL of the candida tropicalis and the Em undiluted suspension to every 200g of herb residue culture medium, and stirring evenly; incubation is taken for 24 to 36 hours at a temperature of 25 to 30 DEG C, a drying process is conducted at a temperature of 60 to 100 DEG C, and lastly a grinding process is conducted; by employing the method provided by the invention for processing the herb residue, the tylosin content in the herb residue is reduced from an original level of 40mg/kg to 60mg/kg to a level of less than 4.5mg/kg, thus leading resource utilization of herb residue to be possible.
Description
Technical field
The present invention relates to a kind of method that reduces tylosin residual in the dregs of a decoction, especially relate to a kind of method of utilizing microbial method to reduce tylosin residual in the dregs of a decoction.
Background technology
Antibiotic dregs of a decoction processing and utilization starts from the later stage fifties both at home and abroad.Nineteen fifty-seven, the back is dried as feed addictive with the tetracycline dregs of a decoction by China Shanghai No. 3 Pharmaceutical Factory, raises pigs in suburb of Shanghai, applies in the chicken industry.Until the latter stage eighties, all places that antibiotics production is arranged are after all the simple solarization of the dregs of a decoction being dried in the air, as protein feed or additive.But owing to be subjected to restrictions such as weather, place, the airing method preliminary treatment dregs of a decoction are subjected to certain restriction.Because can not there be so much airing that comes idly in factory.During the airing, the dregs of a decoction give out a kind of specific peculiar smell, and dregs of a decoction content of organics height easily causes secondary fermentation, and the color blackening produces foul smell, attracts a large amount of flies, and environment is very abominable.Along with the raising of people's environmental consciousness and the increasing of national environmental protection renovation dynamics, over past ten years, people mainly concentrate on the treatment process of the antibiotic dregs of a decoction in the tackling key problem of dry technology.1991, the anti-medical group company in Shandong, Shandong antibiolics dregs dyring process was succeedd.1996, the anti-feed factory in Shandong, Shandong worked out centrifuge again in conjunction with fluidized bed drying penicillin wet slag, made drying cost reduce by 30%.
Along with succeeding in developing of various dregs of a decoction drying equipments, domestic have the man unit of number to carry out the antibiotic dregs of a decoction as high protein feed raw material and medicine Study on Additive.1998～1999, Li Yuehai etc. utilized anti-safe happy mycelian protein in Shandong and cephalo mycelian protein to replace the dregs of beans of equivalent to be used for laying hen with 1.5%, 3% addition respectively and raise, obtain feeding effect preferably; With tylosin protein feed feeding meat chick, fryer survival rate, speed of weight increment, feedstuff-meat ratio all are significantly higher than not interpolation group.
Yet the exploitation of the above-mentioned dregs of a decoction is used, and does not all eliminate antibiotic residual in the dregs of a decoction.Because after animal eats the antibiotic dregs of a decoction of high residue for a long time, can produce all adverse consequences such as drug-fast bacteria, livestock products medicament residue.This just means the dregs of a decoction as the regenerative feedstuff resource, with preceding must reduce in it residual antibiotic.
In recent years, the research report of residual antibiotic degradation technique is very few in the relevant dregs of a decoction both at home and abroad, but it is more to study report in urban domestic wastewater and industrial wastewater aspect the improvement of residual antibiotic.Tang Shaohong etc. directly are used for flowers with the form of fertilizer with the dregs of a decoction and use, and have also obtained satisfied fertilization effect, but residual in the dregs of a decoction antibiotic are arranged, and long-term use may have a negative impact to the soil micro-ecosystem balance.Kummerer etc., Ingerslev etc., Drillia etc. adopt bioanalysis, degradation technique to residual Ciprofloxacin, Ofloxacin, flagyl, SMZco and terramycin for animals etc. in the sanitary wastewater is studied, but resulting result is inconsistent.Main cause is: 1. different antibiotic, and its molecular structure and stability are different; 2. degrade and be still waiting further screening and optimize with bacterial strain; 3. inorganic in the sewage, organic different to different strains degraded different antibiotic influence possibility; 4. temperature, pH value, dissolved oxygen etc. are to the influence of different degradation processes.Lange etc., Andreozzi etc. carry out harmless treatment with ozone to macrolide antibiotics residual in the sewage such as erythromycin, CLA, ROX, lincomycin, obtained comparatively satisfied regulation effect, administer the research of still needing but whether this method is fit to the dregs of a decoction.Also the someone adopts the electrochemical oxidation method to handle antibiotic residue in the waste water, finds can effectively reduce Ofloxacin content in the waste water, but residual lincomycin is not had remarkable degradation, but this method is not suitable for the plant layout processing with the method, because of energy consumption too big.Otker etc., Polubesova etc. adsorb medicines such as residual tetracycline, Enrofloxacin in the sewage with porous type silicate minerals such as montmorillonite, zeolites, and then sewage is purified, but this method is not fundamentally eliminated residual antibiotic, be that change has taken place in the antibiotic site that exists, be not suitable for the improvement of residual antibiotic in the dregs of a decoction.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art, provide a kind of microbial method that utilizes simple, unique, that be easy to promote the use of to reduce the method for tylosin residual in the dregs of a decoction.
The present invention realizes in the following way:
A kind of method of utilizing microbial method to reduce tylosin content residual in the dregs of a decoction, it is characterized in that: this method comprises the steps:
A. dregs of a decoction culture medium preparation: with the raw material of following weight mix get final product dregs of a decoction culture medium: dregs of a decoction 600g～800g, wheat bran 400g～200g, MgSO
47H
2O 0.1g～0.2g, K
2HPO
40.1g～0.2g, FeSO
47H
2O 0.1g～0.3g, water 800mL～1000mL;
B. bacterial suspension preparation: the former dew bacterial strain of heat-obtaining band Candida and Em, be inoculated in respectively in the YPD fluid nutrient medium, cultivate 20～30h in 30 ℃ of shaking tables, behind the centrifugal 15min of 4000r/min, be suspended in the new YPD fluid nutrient medium, can obtain candida tropicalis and the former dew suspension of Em after continuing in 30 ℃ of shaking tables to cultivate 8～10h;
C. get the dregs of a decoction culture medium of step a preparation, the candida tropicalis and the former dew suspension of Em that add step b preparation, each 4～10mL of the former dew suspension of candida tropicalis that adds in the dregs of a decoction culture medium of every 200g and Em, stir, under 25～30 ℃, hatch 24～36h, 60～100 ℃ of oven dry down, get final product after the pulverizing then;
Described YPD fluid nutrient medium is to be mixed by the glucose of the peptone of the yeast extract powder of 5g, 10g, 20g and the water of 1000mL.
The present invention has following effect:
1) reduced the content of residual tylosin in the dregs of a decoction significantly: the present invention adopts microbial method that tylosin herb residue is handled, and makes that residual tylosin content is reduced to below the 4.5mg/kg by original 40mg/kg～60mg/kg in the dregs of a decoction.
2) the present invention utilizes tylosin herb residue again becomes possibility: because the residual quantity of tylosin is higher in the dregs of a decoction, tylosin herb residue is directly used as animal feed or fertilizer, be subjected to extensively doubting of people.Food and drug administration (FDA) regulation, the residual safe level of tylosin in edible tissue and egg is 0.2mg/kg.If is raw material with the dregs of a decoction after the above-mentioned processing, manufacture order cell protein feed or feed enzyme preparation, single cell protein is in 1% ratio, and feed enzyme preparation adds in pig, the chicken perfect compound feed in 0.3% ratio.With the fryer is example, raises the fryer of 2kg, approximately needs the perfect compound feed about 4kg, then consumes dregs of a decoction 0.052kg, and fryer is then eaten the tylosin that is not higher than 0.234mg.If the tylosin in the dregs of a decoction that animal eats all is accumulated in the animal body, then tylosin content is 0.117mg/kg in the fryer body, and the 0.2mg/kg that is lower than the FDA regulation limits the quantity of.In fact, animal eats the dregs of a decoction that contain tylosin, and the tylosin in the dregs of a decoction can not be all by the animal intestinal absorption first; Second through the tylosin of intestinal absorption, major part will be got rid of external through organizing catabolism, has only on a small quantity to be accumulated in the animal tissue.
3) the present invention handles the dregs of a decoction by candida tropicalis and the former dew compound strain of Em, has improved the degradation effect of residual tylosin in the dregs of a decoction.Because candida tropicalis or the former dew of Em will extremely significantly be lower than the effect of the two composite degradation to the degradation effect of residual tylosin in the dregs of a decoction separately.
The specific embodiment
Embodiment 1:
1) dregs of a decoction culture medium preparation
Dregs of a decoction 600g, wheat bran 400g, MgSO
47H
2O 0.1g, K
2HPO
40.1g, FeSO
47H
2O0.1g, water 1000mL.
2) bacterial suspension preparation: the former dew bacterial strain of heat-obtaining band Candida and Em, be inoculated in respectively in the YPD fluid nutrient medium, cultivate 20h in 30 ℃ of shaking tables, behind the centrifugal 15min of 4000r/min, be suspended in the new YPD fluid nutrient medium, continue to cultivate 8h in 30 ℃ of shaking tables.
3) get step 2) preparation candida tropicalis and former each 4mL of dew suspension of Em, be inoculated in the 200g dregs of a decoction culture medium, stir.Under 25 ℃, hatch 36h.Then 60 ℃ of oven dry back pulverizing down.
After measured, tylosin content is 3.2mg/kg in the dregs of a decoction of processing back.
Embodiment 2:
1) dregs of a decoction culture medium preparation
Dregs of a decoction 700g, wheat bran 300g, MgSO
47H
2O, 0.2g, K
2HPO
4, 0.15g, FeSO
47H
2O 0.2g, water 900mL.
2) bacterial suspension preparation: the former dew bacterial strain of heat-obtaining band Candida and Em, be inoculated in respectively in the YPD fluid nutrient medium, cultivate 25h in 30 ℃ of shaking tables, behind the centrifugal 15min of 4000r/min, be suspended in the new YPD fluid nutrient medium, continue to cultivate 9h in 30 ℃ of shaking tables.
3) get step 2) preparation candida tropicalis and former each 7mL of dew suspension of Em, be inoculated in the 200g dregs of a decoction culture medium, stir.Under 28 ℃, hatch 30h.Then 80 ℃ of oven dry back pulverizing down.
After measured, tylosin content is 4.0mg/kg in the dregs of a decoction of processing back.
Embodiment 3:
1) dregs of a decoction culture medium preparation
Dregs of a decoction 800g, wheat bran 200g, MgSO
47H
2O 0.15g, K
2HPO
40.2g, FeSO
47H
2O 0.3g, water 800mL.
2) bacterial suspension preparation: the former dew bacterial strain of heat-obtaining band Candida and Em, be inoculated in respectively in the YPD fluid nutrient medium, cultivate 30h in 30 ℃ of shaking tables, behind the centrifugal 15min of 4000r/min, be suspended in the new YPD fluid nutrient medium, continue to cultivate 10h in 30 ℃ of shaking tables.
3) get step 2) preparation candida tropicalis and former each 10mL of dew suspension of Em, be inoculated in the 200g dregs of a decoction culture medium, stir.Under 30 ℃, hatch 24h.Then 100 ℃ of oven dry back pulverizing down.
After measured, tylosin content is 4.5mg/kg in the dregs of a decoction of processing back.
Above-mentioned YPD is that yeast extract powder peptone liquid of glucose is called for short, and it is that water by the glucose of the peptone of the yeast extract powder of 5g, 10g and 20g and 1000mL mixes.
Claims (1)
1. method of utilizing microbial method to reduce tylosin content residual in the dregs of a decoction, it is characterized in that: this method comprises the steps:
A. dregs of a decoction culture medium preparation: with the raw material of following weight mix get final product dregs of a decoction culture medium: dregs of a decoction 600g～800g, wheat bran 400g～200g, MgSO
47H
2O 0.1g～0.2g, K
2HPO
40.1g～0.2g, FeSO
47H
2O 0.1g～0.3g, water 800mL～1000mL;
B. bacterial suspension preparation: the former dew bacterial strain of heat-obtaining band Candida and Em, be inoculated in respectively in the YPD fluid nutrient medium, cultivate 20～30h in 30 ℃ of shaking tables, behind the centrifugal 15min of 4000r/min, be suspended in the new YPD fluid nutrient medium, can obtain candida tropicalis and the former dew suspension of Em after continuing in 30 ℃ of shaking tables to cultivate 8～10h;
C. get the dregs of a decoction culture medium of step a preparation, the candida tropicalis and the former dew suspension of Em that add step b preparation, each 4～10mL of the former dew suspension of candida tropicalis that adds in the dregs of a decoction culture medium of every 200g and Em, stir, under 25～30 ℃, hatch 24～36h, then 60～100 ℃ of oven dry down, get final product after the pulverizing, described YPD fluid nutrient medium is to be mixed by the glucose of the peptone of the yeast extract powder of 5g, 10g, 20g and the water of 1000mL.
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| CN2007101971953A CN101204613B (en) | 2007-12-06 | 2007-12-06 | Method using microorganism for reducing rudimental tylosin in dregs of a decoction |
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| CN2007101971953A CN101204613B (en) | 2007-12-06 | 2007-12-06 | Method using microorganism for reducing rudimental tylosin in dregs of a decoction |
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| CN101204613A CN101204613A (en) | 2008-06-25 |
| CN101204613B true CN101204613B (en) | 2010-12-15 |
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| CN106694521B (en) * | 2017-01-16 | 2018-09-11 | 河北科技大学 | One metal ion species strengthen method of the thermophilic lysis bacterium for antibiotic bacterium dregs minimizing |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1765530A (en) * | 2005-11-22 | 2006-05-03 | 雷增学 | Method for innocent treatment of antibiotic gruffs by utilizing biological technology |
| CN1778316A (en) * | 2004-11-26 | 2006-05-31 | 广东省新的生物工程研究所有限公司广州生物制品厂 | Degradation of bacterial drug resistance by composite microbial preparation |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1778316A (en) * | 2004-11-26 | 2006-05-31 | 广东省新的生物工程研究所有限公司广州生物制品厂 | Degradation of bacterial drug resistance by composite microbial preparation |
| CN1765530A (en) * | 2005-11-22 | 2006-05-03 | 雷增学 | Method for innocent treatment of antibiotic gruffs by utilizing biological technology |
Non-Patent Citations (4)
| Title |
|---|
| 匡光伟 等.金霉素在鸡粪中的降解研究.《动物医学进展》.2007,第28卷(第6期),50-52. |
| 匡光伟等.金霉素在鸡粪中的降解研究.《动物医学进展》.2007,第28卷(第6期),50-52. * |
| 焦鹏 等.热带假丝酵母不同培养条件下丝化的研究.《微生物学杂志》.2000,第20卷(第2期),30-33. |
| 焦鹏等.热带假丝酵母不同培养条件下丝化的研究.《微生物学杂志》.2000,第20卷(第2期),30-33. * |
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Effective date of registration: 20160513 Address after: 750200 warm springs Industrial Park, Helan County, the Ningxia Hui Autonomous Region, Yinchuan Patentee after: Ningxia hope field biological agriculture science and Technology Co., Ltd. Address before: 750101 the Ningxia Hui Autonomous Region Yongning County Development Zone of Yinchuan City Patentee before: Ningxia Tairui Pharmaceutical Co., Ltd. |