CN101194750A - Toadstool ferment produced perfume for tobacco and method for producing the same - Google Patents
Toadstool ferment produced perfume for tobacco and method for producing the same Download PDFInfo
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- CN101194750A CN101194750A CNA2007100663419A CN200710066341A CN101194750A CN 101194750 A CN101194750 A CN 101194750A CN A2007100663419 A CNA2007100663419 A CN A2007100663419A CN 200710066341 A CN200710066341 A CN 200710066341A CN 101194750 A CN101194750 A CN 101194750A
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Abstract
The invention relates to a tobacco flavor produced by fermenting morel and a preparation process which comprises using morel bevel mycelium as bacterial, activating fluid medium into seed solution before inoculating formation culture medium, and obtaining paste, oil and solid flavoring of tobacco through solvent extracting and low-temperature drying solid culture medium leavening or liquid leavening which uses tobacco pieces, tobacco powder and tea powder as medium. Compared with other tobacco flavors, the tobacco flavor which is extracted after fermenting strengthens the natural fungus aroma ingredients. The volatile ingredient of fungus aroma is added in the extracted paste and oil form tobacco flavor, which perfects flavoring, and plays roles of softening smoke, reducing stimulator and improving aftertaste after adding in cigarettes. And the solid tobacco flavor has permanent fungus aroma, and has the functions of mellowing smoke and adding the aroma of old cigarettes.
Description
Technical field
The present invention relates to the tobacco aromatics using technical field, especially a kind of tobacco aromatics using that utilizes toadstool ferment production and preparation method thereof.
Background technology
Hickory chick (Morchella conica) is the very high rare edible mushroom of a kind of edible medical value, has the fragrance of special happiness.Hickory chick property is flat, flavor is sweet, the beneficial stomach of energy, and phlegm-eliminiating and qi-regulating is good simply traditional Chinese medicine.The discovered in recent years hickory chick contains abundant fungi polysaccharide, and B family vitamin and organic germanium can improve immunity, suppresses myomata 180, and the process that delays senility.
At field of food, there has been multinomial patent to relate to hickory chick: " screening of strain of steepletop hickory chick and the process for preparing strain thereof " patent No.: CN200510048679.2, " screening of balck vein hickory chick bacterial strain and the process for preparing strain thereof " patent No.: CN200510048680.5; Reach the application of hickory chick: " hickory chick seasoning and the production method thereof " patent No.: CN200510135608.6, " morel sauce product and the production method thereof " patent No.: CN200510135610.3 etc. at field of food.
In the tobacco aromatics using technical field, though the extracting method of various mushroom spices is arranged, hickory chick is as the rare edible mushroom of bacterium fragrance uniqueness, because the limitation of resource, hickory chick has not yet to see report as the bacterial classification of exploitation fermented type tobacco aromatics using.
Summary of the invention
The purpose of this invention is to provide and a kind ofly utilize hickory chick to be that strain fermentation produces tobacco aromatics using and its preparation method, this tobacco aromatics using adds to can play soft flue gas in the cigarette, reduce and stimulate, improve pleasant impression and increase the effect of old cigarette perfume (or spice).
The objective of the invention is to be achieved through the following technical solutions:
Adopt the hickory chick bacterial classification, become seed liquor with fluid nutrient medium 15-25 ℃ of activation after 72-120 hour, be added into seed liquor, 15-25 ℃ of fermentation 72-240 hour with the 2-30% of sterilization fermentation culture medium weight, obtain fermentate, fermentate obtains tobacco aromatics using after following method is extracted:
When (1) fermentate is deep fermentation liquid, zymotic fluid directly added hot reflux after, filter, decompression concentrates and obtains bacterium odor type tobacco aromatics using;
When (2) fermentate is solid fermentation Liao, after available 3 times of alcohols solvent refluxing extraction, filter, after vacuum concentrates,
Obtain the tobacco aromatics using that paste body shape has the fragrant composition of bacterium;
When (3) fermentate was solid fermentation Liao, available 2 times of water extracted back 95% precipitation with alcohol twice, after the precipitation low temperature drying, obtained having the solid tobacco aromatics using of the fragrant composition of bacterium;
When (4) fermentate is solid fermentation Liao, available fat-soluble solvent extraction, the extract vacuum obtains transparent fluid shape tobacco aromatics using after concentrating.
The Morchella esculenta (L.) Pers mycelium that hickory chick bacterial classification of the present invention is preserved for the PDA slant medium.
Fluid nutrient medium of the present invention is the water-soluble mixture of sucrose, corn flour, yeast extract, peptone, potassium dihydrogen phosphate, magnesium sulfate etc.
Fermentation substrate of the present invention is a liquid fermentation medium, with the solid mixed culture medium of tobacco leaf fragment, offal and tea dust.
Biofermentation of the present invention is deep fermentation, solid fermentation dual mode.
Alcohols solvent of the present invention is the double solvents of water and ethanol; Described fat-soluble solvent is benzinum, ethyl acetate etc.
Tobacco aromatics using of the present invention is compared with other tobacco aromatics usings and has been strengthened the fragrant composition of the natural bacterium in the tobacco aromatics using, biotransformation through toadstool ferment, increased the fragrant class volatile ingredient of bacterium on lotion that extracts and the oily tobacco aromatics using fragrance component, clear more saturating on fragrance, be added into and can play soft flue gas in the cigarette, reduce the function that stimulates and improve pleasant impression; Solid tobacco aromatics using bacterium after the extraction is fragrant outstanding, is added into the function that tangible pure and mild flue gas is arranged in the cigarette, increase old cigarette perfume (or spice).And hickory chick exploitation tobacco aromatics using scarcity of resources and the too high problem of cost have been solved.
The specific embodiment
Embodiment 1
Picking hickory chick bacterial classification is connected to the PDA inclined-plane, and insulating box is cultivated: 16 ± 1 ℃, 120 hours, treat that mycelia is covered with after, picking is to 200mL fluid nutrient medium (sucrose 3%, peptone 0.1%, yeast extract 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%; 121 ℃ of sterilization 15min), shaken cultivation (16 ± 1 ℃, 120rpm, 96 hours) makes seed liquor.Press zymotic fluid culture medium (sucrose 4%, ammonium sulfate 0.3%, corn flour 0.5%, yeast extract 0.5%, potassium dihydrogen phosphate 0.15%, magnesium sulfate 0.15%; 121 ℃ of sterilization 15min) 5% of weight insert seed liquor, shaken cultivation (18 ± 1 ℃, 120rpm, 96 hours) gets the deep fermentation thing; It filter is collected mycelium pellet, adopts 3 times 30% ethanolic solution refluxing extraction 2 hours, 65 ℃ of decompressions of extract are concentrated, obtain paste body shape hickory chick tobacco aromatics using.
Embodiment 2
Picking hickory chick inclined-plane inoculation is to the PDA inclined-plane, insulating box is cultivated: 21 ± 1 ℃, 96 hours, treat that mycelia is covered with after, picking is to 200mL fluid nutrient medium (sucrose 3%, peptone 0.1%, yeast extract 0.5%, potassium dihydrogen phosphate 0.05%, magnesium sulfate 0.05%; 121 ℃ of sterilization 15min), shaken cultivation (24 ± 1 ℃, 120rpm, 96 hours) makes seed liquor; Use tobacco leaf fragment 1kg, add 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate of tobacco leaf fragment weight, add entry 4kg, 121 ℃ of sterilization 30min, become the solid fermentation culture medium, press 10% of solid fermentation culture medium weight and add seed liquor, 22 ± 1 ℃ of constant temperature culture in back that stir 72 hours become solid fermentation Liao; Solid fermentation Liao was through 3 times 50% ethanolic solution refluxing extraction 2 hours, and 65 ℃ of decompressions of extract concentrate, and obtain paste body shape hickory chick tobacco aromatics using.
Embodiment 3
The preparation of hickory chick seed liquor is used tobacco leaf fragment 1kg with embodiment 2, adds entry 1.5kg, 121 ℃ of sterilization 30min become the solid fermentation culture medium, press 2% interpolation seed liquor of solid fermentation culture medium weight, 20 ± 1 ℃ of constant temperature culture in back that stir 240 hours become solid fermentation Liao.The water refluxing extraction twice that adds 2 times of quality of solid fermentation Liao, each is 1 hour; Concentrate behind the extracting liquid filtering, obtain the paste water extract, add 3 times of quality 95% ethanolic solution precipitations, vacuum filtration with grinding to form the above pressed powder of 100 orders after 60 ℃ of low temperature dryings of sediment employing, promptly gets solid shape hickory chick tobacco aromatics using.
Embodiment 4
The preparation of hickory chick seed liquor is with embodiment 2.Offal 0.5kg, tea dust 1kg, add 0.1% ammonium sulfate, 0.05% potassium dihydrogen phosphate of offal and tea dust gross weight, add entry 6kg, 121 ℃ of sterilization 30min get the solid fermentation culture medium, press 30% of solid fermentation culture medium weight and add seed liquor, 16 ± 1 ℃ of constant temperature culture 120 hours become solid fermentation Liao; Solid fermentation Liao is through 3 times of benzinums (or ethyl acetate or the two mixture) extraction 3 times, and extract normal temperature concentrates for 70 ℃ and obtains oily hickory chick tobacco aromatics using.
Embodiment 5
The preparation of hickory chick seed liquor is with embodiment 2.Offal 0.5kg, tea dust 1kg adds entry 2.25kg water, gets the solid fermentation culture medium, presses 15% of solid fermentation culture medium weight and adds seed liquor, and 20 ± 1 ℃ of constant temperature culture in back that stir 216 hours become solid fermentation Liao.Add 2 times of quality water of solid fermentation Liao refluxing extraction twice, each is 1 hour; Concentrate behind the extracting liquid filtering, obtain the paste water extract, add the 95% ethanolic solution precipitation of 3 times of paste water extract weight, filter; Filter residue adopts 10 times of water Hui Rong of its weight, refilters, and 65 ℃ of decompressions of filtrate concentrate, and obtain condensed cream, add 95% ethanolic solution precipitation again, filter, and filter residue grinds to form the above pressed powder of 100 orders after adopting 40 ℃ of low temperature dryings, promptly gets solid hickory chick tobacco aromatics using.
Claims (7)
1. the tobacco aromatics using produced of a toadstool ferment, it is characterized in that preparing by the following method: adopting the hickory chick mycelia is starting strain, become seed liquor with fluid nutrient medium 15-25 ℃ of activation after 72-120 hour, 2-30% with fermentation medium weight is added into seed liquor, 15-25 ℃ fermented 72-240 hour, and obtained fermentate one deep fermentation liquid or solid fermentation Liao; After deep fermentation liquid then directly adds hot reflux by zymotic fluid, filter, decompression concentrates and obtains bacterium odor type tobacco aromatics using; By after the alcohols solvent refluxing extraction, filter when fermentate is solid fermentation Liao, obtain the tobacco aromatics using that paste body shape has the fragrant composition of bacterium after vacuum concentrates; Or, obtain having the solid tobacco aromatics using of the fragrant composition of bacterium after the precipitation employing low temperature drying by twice back precipitation with alcohol of water extraction; Or by the fat-soluble solvent extraction, the extract vacuum obtains the transparent tobacco aromatics using of flow-like after concentrating.
2. the tobacco aromatics using that toadstool ferment according to claim 1 is produced is characterized in that: the Morchella esculenta (L.) Pers mycelium that described hickory chick bacterial classification is preserved for the PDA slant medium.
3. the tobacco aromatics using that toadstool ferment according to claim 1 is produced, it is characterized in that: described solid fermentation culture medium is: the 20-40% tobacco leaf fragment, or on the tobacco leaf fragment basis, add 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate of its weight again, all the other are made for water.
4. the tobacco aromatics using that toadstool ferment according to claim 3 is produced, it is characterized in that: described solid fermentation culture medium is: the 20-40% tobacco leaf fragment, add 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate of tobacco leaf fragment weight again, all the other are made for water.
5. the tobacco aromatics using that toadstool ferment according to claim 1 is produced, it is characterized in that: described solid fermentation culture medium is: 20-40% offal and tea dust, offal mixes in 1: 2 ratio with tea dust, and all the other are made for water.
6. the tobacco aromatics using that toadstool ferment according to claim 5 is produced, it is characterized in that: described solid fermentation culture medium is: 20-40% offal and tea dust, offal mixes in 1: 2 ratio with tea dust, add 0.1% ammonium sulfate, 0.05% potassium dihydrogen phosphate of offal and tea dust weight again, all the other are made for water.
7. the tobacco aromatics using that toadstool ferment according to claim 1 is produced, it is characterized in that: described alcohols solvent is the double solvents of water and ethanol, described fat-soluble solvent is benzinum, ethyl acetate.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2007100663419A CN101194750A (en) | 2007-11-02 | 2007-11-02 | Toadstool ferment produced perfume for tobacco and method for producing the same |
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| CNA2007100663419A CN101194750A (en) | 2007-11-02 | 2007-11-02 | Toadstool ferment produced perfume for tobacco and method for producing the same |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101480274B (en) * | 2009-02-20 | 2011-07-27 | 王乐观 | Method for fermenting tobacco leaf using Pu'er tea |
| CN101449854B (en) * | 2008-12-30 | 2011-12-21 | 无锡华馨香精香料有限公司 | Fermentation optimization method of tobacco essence |
| CN102499462A (en) * | 2011-11-21 | 2012-06-20 | 云南瑞升烟草技术（集团）有限公司 | Method for preparing tobacco perfume by pile-fermenting tobacco stems by utilizing resting cells |
| CN102851241A (en) * | 2012-09-05 | 2013-01-02 | 湖北中烟工业有限责任公司 | Preparation method for irritation reducing fermentation tobacco extract, and applications of irritation reducing fermentation tobacco extract in recombinant tobacco |
| CN102920016A (en) * | 2012-11-09 | 2013-02-13 | 成都宏亿实业集团有限公司 | Porcini extract and preparation method and application thereof |
| CN102978127A (en) * | 2012-09-05 | 2013-03-20 | 湖北中烟工业有限责任公司 | Tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf |
| CN105779307A (en) * | 2016-04-18 | 2016-07-20 | 云南中烟工业有限责任公司 | Trichoderma aureoviride strain and method for preparing Pu'er Tea tobacco flavor |
| CN107384591A (en) * | 2017-03-22 | 2017-11-24 | 贵州鼎立生物科技香料有限公司 | A kind of preparation method of cigarette essence |
| CN108913608A (en) * | 2018-07-24 | 2018-11-30 | 河北民族师范学院 | A kind of storage medium and method for preserving preventing hickory chick spawn degeneration |
-
2007
- 2007-11-02 CN CNA2007100663419A patent/CN101194750A/en active Pending
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101449854B (en) * | 2008-12-30 | 2011-12-21 | 无锡华馨香精香料有限公司 | Fermentation optimization method of tobacco essence |
| CN101480274B (en) * | 2009-02-20 | 2011-07-27 | 王乐观 | Method for fermenting tobacco leaf using Pu'er tea |
| CN102499462B (en) * | 2011-11-21 | 2013-08-21 | 云南瑞升烟草技术（集团）有限公司 | Method for preparing tobacco perfume by pile-fermenting tobacco stems by utilizing resting cells |
| CN102499462A (en) * | 2011-11-21 | 2012-06-20 | 云南瑞升烟草技术（集团）有限公司 | Method for preparing tobacco perfume by pile-fermenting tobacco stems by utilizing resting cells |
| CN102851241A (en) * | 2012-09-05 | 2013-01-02 | 湖北中烟工业有限责任公司 | Preparation method for irritation reducing fermentation tobacco extract, and applications of irritation reducing fermentation tobacco extract in recombinant tobacco |
| CN102978127A (en) * | 2012-09-05 | 2013-03-20 | 湖北中烟工业有限责任公司 | Tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf |
| CN102851241B (en) * | 2012-09-05 | 2013-12-18 | 湖北中烟工业有限责任公司 | Preparation method for irritation reducing fermentation tobacco extract, and applications of irritation reducing fermentation tobacco extract in recombinant tobacco |
| CN102978127B (en) * | 2012-09-05 | 2014-06-11 | 湖北中烟工业有限责任公司 | Tobacco leaf extract and cocoa extract composite fermentation perfume preparation method and applications of tobacco leaf extract and cocoa extract composite fermentation perfume in recombinant tobacco leaf |
| CN102920016A (en) * | 2012-11-09 | 2013-02-13 | 成都宏亿实业集团有限公司 | Porcini extract and preparation method and application thereof |
| CN102920016B (en) * | 2012-11-09 | 2014-12-10 | 成都宏亿实业集团有限公司 | Porcini extract and preparation method and application thereof |
| CN105779307A (en) * | 2016-04-18 | 2016-07-20 | 云南中烟工业有限责任公司 | Trichoderma aureoviride strain and method for preparing Pu'er Tea tobacco flavor |
| CN105779307B (en) * | 2016-04-18 | 2019-04-05 | 云南中烟工业有限责任公司 | A kind of trichoderma aureoviride bacterial strain and the method for preparing Pu'er tea tobacco aromaticss with it |
| CN107384591A (en) * | 2017-03-22 | 2017-11-24 | 贵州鼎立生物科技香料有限公司 | A kind of preparation method of cigarette essence |
| CN108913608A (en) * | 2018-07-24 | 2018-11-30 | 河北民族师范学院 | A kind of storage medium and method for preserving preventing hickory chick spawn degeneration |
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Open date: 20080611 |