CN101185682B - Medicinal composition for treating constipation and preparation method and application thereof - Google Patents
Medicinal composition for treating constipation and preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a drug combination used for treating constipation, which is an agent made from the raw materials with the following weight proportions: 1-3 portions of rhubarb and 1-3 portions of aconite. The invention also provides the preparation method and application of the drug combination. The drug effect of the invention is clear. The raw materials utilize the compatibility of the effective components of aconite and rhubarb, for example, the compatibility of the total alkaloid of aconite and the total anthraquinone of rhubarb or the bound anthraquinone of rhubarb can treat constipation, which not only has the best drug efficient compatibility and strong quality controllability but also provides a new choice for clinic.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition for the treatment of constipation, particularly, the effective site that is medicine is the pharmaceutical composition that active component is prepared from, and belongs to the field of Chinese medicines.
Background technology
The compatibility application head of Radix Et Rhizoma Rhei and Radix Aconiti Lateralis Preparata sees the Eastern Han Dynasty and opens machine " Medical Treasures of the Golden Chamber abdominal distention colic of cold type dyspepsia abnormal pulse card controls the tenth " year dahuang fuzi decoction, captures with the Radix Et Rhizoma Rhei bitter cold, and the hot cold expelling of Radix Aconiti Lateralis Preparata, simultaneously using both cold and hot drugs opposes each other and yet also complement each other, the initiative relaxing the bowels with purgatives of warm nature beginning.Its modern pharmacological research: strengthen bowel movement: Jin Shi and studies have shown that by experiment, Radix Et Rhizoma Rhei is compared with Radix Et Rhizoma Rhei or Radix Aconiti Lateralis Preparata with single with the Radix Aconiti Lateralis Preparata compatibility, and cold accumulation and constipation type mice defecation time is obviously shortened, and the feces volume showed increased strengthens enterokinesia simultaneously.Dahuang fuzi decoction decocting liquid low dose has tangible excitation to the rabbit intestine in vitro, and this effect is not blocked by atropine.Strengthen anti-anoxia ability: Li Shi and studies confirm that by experiment, Radix Et Rhizoma Rhei Radix Aconiti Lateralis Preparata water alcohol method extracting solution can obviously prolong and causes the time-to-live that the whole anoxia of mice and ligation neck arteries cause mouse brain hypoxic-ischemic animal under the normal pressure; Potassium cyanide and sodium nitrite poisoning induced cell hypoxia there is protective effect; Can resist isoproterenol and cause anoxia in mice.The thermoregulation effect: having animal experiment study to show, is under 10 ℃ of conditions in temperature, and dahuang fuzi decoction is pressed 0.2ml/10g and irritated cold syndrome of the stomach accumulation and constipation type mice, respectively at before the administration and surveyed toes and the rectal temperature of mice after the administration in 1 hour.The result: dahuang fuzi decoction has remarkable antagonism to the toes temperature decline of mice.
Modern clinical practice adds the various acute abdomen of flavor treatment in acute abdomen: Ceng Shi with dahuang fuzi decoction: acute edematous pancreatitis adds Rhizoma Pinelliae 9g, Caulis Bambusae In Taenia 12g, Fructus Toosendan 12g, Fructus Aurantii Immaturus 15g, Radix Aucklandiae (Radix Vladimiriae) 9g, Radix Glycyrrhizae Preparata 9g etc.; And there is dahuang fuzi decoction to add the case of testing of flavor treatment acute ileus, acute cholelithiasis.Intestinal obstruction: Li Shi etc. are main treatment intestinal obstruction 21 examples with the dahuang fuzi decoction.Prescription is Radix Et Rhizoma Rhei 10g, Radix Aconiti Lateralis Preparata 6g, Herba Asari 3g, Semen Raphani 12g, Pericarpium Arecae 16g.If the intenseness of heat person adds Herba Patriniae, Radix Scutellariae, Fructus Gardeniae; Malnutrition due to parasitic infestation person is with Semen Arecae, Fructus Mume, another name for Sichuan Province green pepper, Fructus Toosendan; Vomit frequent person and add Haematitum, Caulis Bambusae In Taenia.All cooperate acupuncture bilateral tsu san li.As a result, 21 routine patients all cure.Chronic colitis: Zhou Shi adds flavor treatment chronic non-specific ulcerative colitis 27 examples with Radix Et Rhizoma Rhei, Radix Aconiti Lateralis Preparata compatibility with card, and wherein, cold-damp is gathered 16 examples, 5 examples that liver-yang hyperactivity is insufficiency of the spleen, deficiency of spleen-YANG and kidneyYANG 4 examples, damp-heat accumulation 2 examples.As a result, 16 routine cold-damp accumulators cure 11 examples, 4 examples that take a turn for the better, invalid 1 example; 5 routine liver-yang hyperactivity person with Spleen Deficiency are cured 3 examples, 1 example that takes a turn for the better, invalid 1 example; 4 routine deficiency of spleen-YANG and kidneyYANG persons cure 2 examples; 2 routine damp-heat accumulation persons cure 1 example, 1 example that takes a turn for the better, and total effective rate is 92.5%.Duodenal bulbar ulcer: the Li Ke old docter of TCM that continues treats 1 routine duodenal bulbar ulcer person with dahuang fuzi decoction, sees that pain is frequent, chest pain radiating to the back, or connect both sides of the chest, and acutely unbearably, constipation, pulse condition sink the person, obey 1 dose, i.e. free movement of the bowels, the pain people that disappears.Also all get effect with this side's treatment intractable stomachache, ascaris intestinal obstruction etc.Acute cholecystitis: Xu Shi treats acute cholecystitis 25 examples with dahuang fuzi decoction.Side's medicine consists of Radix Et Rhizoma Rhei 10g, Radix Aconiti Lateralis Preparata 15g, Herba Asari 2g.As a result, cure 16 examples, account for 64%; 7 examples that take a turn for the better account for 28%; Invalid 2 examples account for 8%; Total effective rate reaches 92%.Constipation of old people: Li Shi etc. are with giving birth to the type constipation in insufficiency of the spleen depressed, the excess-heat of dahuang fuzi decoction plus-minus treatment old people.Prescription is Radix Aconiti Lateralis Preparata 10g, Radix Et Rhizoma Rhei 10g, and Natrii Sulfas 3g, Poria 9g, Rhizoma Atractylodis Macrocephalae 15g, Radix Aucklandiae 9g,, fry cedar seed 9g, Radix Angelicae Sinensis 9g.Obey 1 dose, stool promptly down, no abdominal pain diarrhea, 7 doses of follow-up clothes, every day 1-2 time of defecating, the diet amelioration is advised its modest movement, the informal dress Renshen Guipi Wan is followed up a case by regular visits to January, it is all normal to defecate.To carry out 340 preceding routine patients of colono-fibrescopy as intestinal cleaning agents: Lv Shi report, and be divided into 4 groups at random and make intestinal tract cleaning, Chinese drug-treated group 160 examples, 4h hour clothes plus-minus dahuang fuzi decoction 1000ml (having drunk in the 10min) before the microscopy; Matched group is respectively Oleum Ricini 100 examples; Mannitol 50 examples; Magnesium sulfate 30 examples.As a result, Chinese drug-treated group obviously is better than each matched group.Clinical renal failure, diabetic nephropathy, uremia, urinary system calculus, nervous headache, primary sciatica, varicose veins of the lower extremity pain, the hypochondriac pain etc. of also treating.
But do not see the report of Radix Aconiti Lateralis Preparata Radix Et Rhizoma Rhei effective site treatment constipation, more do not see the report of best proportion compatibility of Radix Aconiti Lateralis Preparata Radix Et Rhizoma Rhei effective site and combination treatment constipation.
Summary of the invention
Technical scheme of the present invention has provided a kind of pharmaceutical composition for the treatment of constipation, and another technical scheme of the present invention has provided this preparation of drug combination method.
The invention provides a kind of pharmaceutical composition for the treatment of constipation, it is the medicament that is prepared from by the following weight proportion raw material:
Radix Et Rhizoma Rhei 1-3 part, Radix Aconiti Lateralis Preparata 1-3 part.
Wherein, it is to be the medicament that active component is prepared from by Radix Et Rhizoma Rhei effective site, Radix Aconiti Lateralis Preparata effective site:
Radix Et Rhizoma Rhei effective site 1-3 part, Radix Aconiti Lateralis Preparata effective site 1-3 part, wherein said Radix Et Rhizoma Rhei effective site is total Radix Et Rhizoma Rhei anthraquinone or rhubarb-combined anthraquinone; Radix Aconiti Lateralis Preparata effective site is Radix Aconiti Lateralis Preparata total alkaloids, and described Radix Aconiti Lateralis Preparata total alkaloids is in aconitine content 〉=30%w/w; Total Radix Et Rhizoma Rhei anthraquinone is with 1,8 dihydroxyanthraquinone meter content 〉=40%w/w; Rhubarb-combined anthraquinone is with 1,8 dihydroxyanthraquinone meter content 〉=30%w/w.
Total Radix Et Rhizoma Rhei anthraquinone, rhubarb-combined anthraquinone, Radix Aconiti Lateralis Preparata total alkaloids can extract preparation by textbook or document disclosed method, as Xiao Chonghou. Chemistry for Chinese Traditional Medicine. and the .1997 of Shanghai science tech publishing house; Zhang Yonghong. the selection of anthraquinone component extraction conditions in the Radix Et Rhizoma Rhei. northwest pharmaceutical journal, 1997; 12 (2): 59～60; Wang Huichun opens into total. the extraction of Rheum tanguticum anthraquinone derivative with separate. Qinghai Normal University's journal (natural science edition), 2006; (4): 89～92; Zhao Weilei, Tang Xing. the extraction and purification process of Radix Aconiti Lateralis Preparata total alkaloids. Shenyang Pharmaceutical University's journal, 2007; 24 (7): 433～435.
Wherein, it is the medicament that the feedstock production by the following weight compatibility forms:
Radix Aconiti Lateralis Preparata total alkaloids: total Radix Et Rhizoma Rhei anthraquinone=1: 2-1: 1.
Wherein, it is the medicament that the feedstock production by the following weight compatibility forms:
Radix Aconiti Lateralis Preparata total alkaloids: rhubarb-combined anthraquinone=2: 1-1: 1.
Described medicament is tablet, granule, capsule, pill, oral liquid.
The present invention also provides the method for this pharmaceutical composition, comprises the steps:
A, take by weighing materials of weight proportions:
Radix Et Rhizoma Rhei 1-3 part, Radix Aconiti Lateralis Preparata 1-3 part;
Effective site in b, the extraction raw material, wherein, Radix Et Rhizoma Rhei effective site is total Radix Et Rhizoma Rhei anthraquinone or rhubarb-combined anthraquinone; Radix Aconiti Lateralis Preparata effective site is Radix Aconiti Lateralis Preparata total alkaloids;
C, mixing add acceptable accessories or complementary composition and are prepared into preparation pharmaceutically commonly used.
The present invention also provides the purposes of this pharmaceutical composition in the medicine of preparation treatment constipation.
Medicine effect of the present invention is clear and definite, and raw material is Radix Aconiti Lateralis Preparata, effective rhubarb component compatibility, as with Radix Aconiti Lateralis Preparata total alkaloids and total Radix Et Rhizoma Rhei anthraquinone or rhubarb-combined anthraquinone compatibility, effect with treatment constipation, and have the optimum medicine efficacy compatibility, quality controllability is strong, provides a kind of new selection for clinical.
The specific embodiment
The preparation of embodiment 1 medicine of the present invention
Get raw material Radix Et Rhizoma Rhei 300g, Radix Aconiti Lateralis Preparata 300g, extract the effective site in the raw material, wherein, Radix Et Rhizoma Rhei effective site is total Radix Et Rhizoma Rhei anthraquinone or rhubarb-combined anthraquinone; Radix Aconiti Lateralis Preparata effective site is Radix Aconiti Lateralis Preparata total alkaloids; The effective site for preparing is mixed, add starch and granulate, add magnesium stearate, tabletting gets tablet.
The preparation of embodiment 2 medicines of the present invention
Get raw material Radix Et Rhizoma Rhei 100g, Radix Aconiti Lateralis Preparata 300g, directly decoct with water, concentrate, granulate, encapsulated, get capsule.
The preparation of embodiment 3 medicines of the present invention
Get raw material Radix Et Rhizoma Rhei 300g, Radix Aconiti Lateralis Preparata 100g, press the method for embodiment 1 and extract effective site, get wherein Radix Aconiti Lateralis Preparata total alkaloids, rhubarb-combined anthraquinone mixes, and granulates, and is encapsulated, gets capsule.
The preparation of embodiment 4 medicines of the present invention
Get commercially available Radix Aconiti Lateralis Preparata total alkaloids 10g; Total Radix Et Rhizoma Rhei anthraquinone 20g adds starch, dextrin, granulates, and tabletting gets tablet.
The preparation of embodiment 5 medicines of the present invention
Get commercially available Radix Aconiti Lateralis Preparata total alkaloids 10g, total Radix Et Rhizoma Rhei anthraquinone 10g adds starch, dextrin, granulates, and tabletting gets tablet.
The preparation of embodiment 6 medicines of the present invention
Get commercially available Radix Aconiti Lateralis Preparata total alkaloids 20g; Rhubarb-combined anthraquinone 10g adds starch, dextrin, granulates, and tabletting gets tablet.
The preparation of embodiment 7 medicines of the present invention
Get commercially available Radix Aconiti Lateralis Preparata total alkaloids 10g, rhubarb-combined anthraquinone 10g adds starch, dextrin, granulates, and tabletting gets tablet.
Below prove beneficial effect of the present invention by concrete pharmacodynamics test.
Test example 1 Radix Aconiti Lateralis Preparata total alkali and total Radix Et Rhizoma Rhei anthraquinone different proportion compatibility are to the influence of intact animal's defecation
1 experiment material
1.1 experiment medicine
Be subjected to the reagent thing, the Radix Aconiti Lateralis Preparata total alkali, every 1ml contains the 1kg crude drug, and the every 1ml of total Radix Et Rhizoma Rhei anthraquinone contains the 1.2g crude drug.Before being made into 1: 1,1: 2,2: 1 medicinal liquids, total alkali becomes every 1ml to be equivalent to the 1.0g crude drug with the buffer of pH6.8 respectively with total quinone with distilled water diluting.
1.2 laboratory animal
The healthy adult SD rat, body weight 190～210g.Laboratory animal production licence number: 04-11.Provide by Chengdu University of Traditional Chinese Medicine animal experiment study center, the quarantine back is standby.
2 experimental techniques
Following total alkali is the Radix Aconiti Lateralis Preparata total alkali, and general anthraquinone is a total Radix Et Rhizoma Rhei anthraquinone.
Get 36 of healthy adult SD rats, be divided into blank group (N=6), total alkali group (N=6), general anthraquinone group (N=6), total alkali at random: general anthraquinone (2: 1) group (N=6), total alkali: general anthraquinone (1: 1) group (N=6), total alkali: general anthraquinone (1: 2) group (N=6) 6 groups.Behind the fasting 12h, each group is pressed 10ml/kg, 5.0ml/kg, 10ml/kg, 7.5ml/kg, 10ml/kg, 15ml/kg filling stomach normal saline, total alkali, general anthraquinone, total alkali respectively: general anthraquinone=2: 1,1: 1,1: 2 medicinal liquid, after irritating stomach, immediately rat is pursued and only put into metabolic cage, place mat A4 copy paper under the metabolic cage, drinking water supply is observed the gross weight that voids excreta in its first grain time that voids excreta and its 3h that weighs, and is write down just matter.Test data is handled with the SPSS11.0 statistical software.Stool weight=A4 copy paper and stool weight-A4 copy paper weight.
3 experimental results
The molding of blank treated animal feces, oblong, yellow skin, yellowish green, light brown or black.The most passage of loose stools of administration treated animal, accidental shaped granule shape feces.Compare with the blank group: general anthraquinone group, total alkali: general anthraquinone=1: 1 group and total alkali: general anthraquinone=gross weight that voids excreta in the treated animal 3h had respectively significantly or utmost point significant difference in 1: 2; Total alkali: the first grain defecation time of general anthraquinone=1: 2 and 1: 1 treated animal has significant difference.3 proportion compatibilities of Radix Aconiti Lateralis Preparata total alkali and total Radix Et Rhizoma Rhei anthraquinone with single with total alkali, single with general anthraquinone relatively: total alkali: general anthraquinone=1: 1 group and total alkali: the gross weight that voids excreta in the first grain defecation time of general anthraquinone=1: 2 treated animal and the 3h has remarkable or utmost point significant difference respectively, wherein, total alkali: the feces gross weight of general anthraquinone=2: 1 treated animal significantly is less than singly uses general anthraquinone, and detailed results sees Table 1.
Table 1 total alkali and total quinone compatibility are to the influence of normal rat defecation
| Group | n | Dosage/mlkg -1 | Body weight | First grain defecation time/min | Defecation weight/g in the 3h |
| The total quinone group of blank group total alkali group total alkali: total quinone (2: 1) total alkali: total quinone (1: 1) total alkali: total quinone (1: 2) | 6 6 6 6 6 6 | 10.0 5.0 10.0 7.5 10.0 15.0 | 205.33±6.53202.50±?5.68202.67±?7.37204.50±4.77205.50±5.32204.50±7.42 | 77.17±39.60 67.17±44.29 38.50±24.76 47.17±22.46 34.33±21.32 1) 18.00±4.73 1)3)7) | 1.79±0.72 2.47±0.76 3.41±0.46 1) 2.52±0.73 6) 4.53±0.99 2)4)7) 5.85±0.17 2)5)8) |
Compare with the blank group
1)P＜0.05,
2)P＜0.001.Ratio group and total alkali group are relatively
3)P＜0.05,
4)P＜0.01,
5)P＜0.001.Ratio group and general anthraquinone group are relatively
6=P＜0.05,
7=P＜0.01,
8=P＜0.001.
4 experiment conclusion
The experimental result prompting, single all have the first grain of the animal of shortening defecation time trend with total alkali or general anthraquinone, and list can obviously promote the animal feces volume with general anthraquinone; Total alkali and general anthraquinone compatibility 1: 1 and 1: 2 ratio have significant shortening animal first grain defecation time and promote defecation, and 2: 1 ratios of total alkali and general anthraquinone compatibility promote the animal defecation not single effective with general anthraquinone.Experimental result is also pointed out, and total alkali and general anthraquinone compatibility have certain synergistic function to intact animal's defecation, and the first grain of animal defecation time shortens with the increase of general anthraquinone ratio, and the gross weight that voids excreta in the animal 3h increases weight with the increase of general anthraquinone ratio.
Total alkali, general anthraquinone, total alkali: 3 proportion compatibilities of general anthraquinone promote the strong and weak order of animal defecation to be followed successively by total alkali: 1: 2＞total alkali of general anthraquinone: 1: 1＞general anthraquinone＞total alkali of general anthraquinone: 2: 1＞total alkali of general anthraquinone.
The different proportion compatibilities with total Radix Et Rhizoma Rhei anthraquinone of test example 2 Radix Aconiti Lateralis Preparata total alkalis are to the influence of yang deficiency constipation rat model defecation
1 experiment material
1.1 experiment medicine
Be subjected to the reagent thing, the Radix Aconiti Lateralis Preparata total alkali, every 1ml contains the 1kg crude drug, and the every 1ml of total Radix Et Rhizoma Rhei anthraquinone contains the 1.2g crude drug.Before being made into 1: 1,1: 2,2: 1 medicinal liquids, total alkali becomes every 1ml to be equivalent to the 1.0g crude drug with the buffer of pH6.8 respectively with total quinone with distilled water diluting.
The modeling medicine, the Shanxi white vinegar, total acidity＞4.5g/100ml is produced by Shanxi mature vinegar factory.Active carbon, east, Chongqing examination chemical industry company limited is produced.Ice is from freezing.
1.2 laboratory animal
The healthy adult SD rat, body weight 200～220g.Laboratory animal production licence number: 04-11.Provide by Chengdu University of Traditional Chinese Medicine animal experiment study center, the quarantine back is standby.
2 experimental techniques
Get 42 of healthy adult SD rats, be divided into blank group (N=6), model control group (N=6), total alkali group (N=6), general anthraquinone group (N=6), total alkali at random: general anthraquinone (2: 1) group (N=6), total alkali: general anthraquinone (1: 1) group (N=6), total alkali: general anthraquinone (1: 2) group (N=6) 7 groups.Except that the blank group, other each treated animals are all irritated stomach by first dosage 20ml/kg and are given Shanxi white vinegar and 0 ℃ of active carbon frozen water, twice modeling administration time is at a distance of 4h, continuous modeling 9d, modeling simultaneously, each treated animal is pressed 10ml/kg every day respectively, 10ml/kg, 5.0ml/kg, 10ml/kg, 7.5ml/kg, 10ml/kg, 15ml/kg irritates the stomach normal saline, normal saline, total alkali, general anthraquinone, total alkali: general anthraquinone=2: 1,1: 1,1: 2 medicinal liquid, continuous 9d, weigh, after the last administration, immediately rat is pursued and only put into metabolic cage, place mat A4 copy paper under the metabolic cage, drinking water supply is observed the gross weight that voids excreta in its first grain time that voids excreta and its 3h that weighs, and is write down just matter.Test data is handled with the SPSS11.0 statistical software.
3 experimental results
The molding of blank treated animal feces, oblong, yellow skin, yellowish green, light brown or black.Model group animal row black, molding, slightly hard dried matter feces.The administration treated animal respectively passage of loose stools, shaped granule shape just or both hold concurrently mutually.Compare with the blank group: the first grain of model group animal defecation time obviously postpones, and the gross weight that voids excreta in the 3h is less than the blank group, but not statistically significant.Compare with model control group: total alkali group, general anthraquinone group, total alkali: the gross weight that voids excreta in the first grain defecation time of each ratio treated animal of general anthraquinone and the 3h has respectively significantly or utmost point significant difference.With single with the total alkali group relatively: total alkali: the gross weight that voids excreta in the 3h of general anthraquinone=2: 1 and 1: 1 treated animal has significant difference, and total alkali: the gross weight that voids excreta in the 3h of general anthraquinone=1: 2 treated animal also has increase trend, and detailed results sees Table 2.
Table 2 total alkali and total quinone compatibility are to the influence of yang deficiency constipation rat model defecation
| Group | n | Dosage/mlkg -1 | Body weight before the experiment | Experiment back body weight | First grain defecation time/min | Defecation weight/g in the 3h |
| The total quinone group of blank group model group total alkali group total alkali: total quinone (2: 1) total alkali: total quinone (1: 1) total alkali: total quinone (1: 2) | 6666666 | 10.0 10.0 5.0 10.0 7.5 10.0 15.0 | 209.50±4.51 210.17±3.43 209.83±2.64 209.00±4.90 209.83±2.48 209.83±3.54 209.00±3.41 | 221.50±3.94 216.50±4.51 214.33±6.56 211.17±3.76 213.33±2.42 212.00±4.00 211.33±4.03 | 69.33±39.64146.50±50.16 1)77.67±53.60 2)35.00±11.06 3)49.17±20.66 3)23.67±11.55 3)31.33±27.02 3) | 1.57±0.21 0.93±1.03 1.31±0.65 2.28±0.65 2) 2.86±0.32 3)5) 2.08±0.32 2)4) 1.89±0.35 |
Compare with the blank group
1)P＜0.05.Compare with model group
2)P＜0.05,
3)P＜0.01.Proportion compatibility group and total alkali group are relatively
4=P＜0.05,
5=P＜0.01.
4 experiment conclusion
The experimental result prompting, single have the first grain of obvious shortening animal pattern defecation time and the trend that increases the gross weight that voids excreta in the animal pattern 3h with total alkali.Single have obvious shortening animal pattern first grain defecation time and significantly increase the gross weight effect that voids excreta in the animal pattern 3h with general anthraquinone.Total alkali and general anthraquinone compatibility 1: 1 and 2: 1 ratios have remarkable shortening animal first grain defecation time and obviously increase the gross weight effect that voids excreta in the animal pattern 3h, and total alkali and always fear the trend that 1: 2 ratio of quinone compatibility has the gross weight that voids excreta in obvious shortening animal pattern first grain defecation time and the increase animal pattern 3h.Experimental result is also pointed out, total alkali and general anthraquinone compatibility 1: 1 and 2: 1 ratios with the total alkali group relatively have certain synergistic function to the defecation of animal pattern with single, but, each proportion compatibility of total alkali and general anthraquinone compares with the general anthraquinone group with single, there is no synergistic function.
Total alkali: general anthraquinone compatibility 1: 1 and 2: 1 ratio group promotion animal pattern defecation intensity are better than list and use the total alkali group, and total alkali: each proportion compatibility group of general anthraquinone is not seen notable difference with single with the general anthraquinone group to the influence of animal pattern defecation.
Test example 3 Radix Aconiti Lateralis Preparata total alkalis and rhubarb-combined anthraquinone different proportion compatibility are to the influence of intact animal's defecation
1 experiment material
1.1 experiment medicine
Be subjected to the reagent thing, the Radix Aconiti Lateralis Preparata total alkali, every 1ml contains the 1kg crude drug, and the every 1ml of combined anthraquinone contains the 1.2g crude drug.Before being made into 1: 1,1: 2,2: 1 medicinal liquids, total alkali becomes every 1ml to be equivalent to the 1.0g crude drug with the buffer of pH6.8 respectively with total quinone with distilled water diluting.
1.2 laboratory animal
The healthy adult SD rat, body weight 190～210g.Laboratory animal production licence number: 04-11.Provide by Chengdu University of Traditional Chinese Medicine animal experiment study center, the quarantine back is standby.
2 experimental techniques
Get 36 of healthy adult SD rats, be divided into blank group (N=6), total alkali group (N=6), combined anthraquinone group (N=6), total alkali at random: combined anthraquinone (2: 1) group (N=6), total alkali: combined anthraquinone (1: 1) group (N=6), total alkali: combined anthraquinone (1: 2) group (N=6) 6 groups.Behind the fasting 12h, each group is pressed 10ml/kg, 5.0ml/kg, 10ml/kg, 7.5ml/kg, 10ml/kg, 15ml/kg filling stomach normal saline, total alkali, combined anthraquinone, total alkali respectively: combined anthraquinone=2: 1,1: 1,1: 2 medicinal liquid, after irritating stomach, immediately rat is pursued and only put into metabolic cage, place mat A4 copy paper under the metabolic cage, drinking water supply is observed the gross weight that voids excreta in its first grain time that voids excreta and its 3h that weighs, and is write down just matter.Test data is handled with the SPSS11.0 statistical software.Stool weight=A4 copy paper and stool weight-A4 copy paper weight.
3 experimental results
The molding of blank treated animal feces, oblong, yellow skin, yellowish green, brown or black.The administration treated animal is arranged normal molding just mostly, accidental light stool in heaps.With the blank group relatively: combined anthraquinone group, total alkali: the gross weight that voids excreta in the first grain defecation time of combined anthraquinone=1: 1,1: 2,2: 1 treated animals and the 3h has respectively significantly or utmost point significant difference; Compare with Radix Aconiti Lateralis Preparata total alkali group with single: the gross weight that voids excreta in the first grain defecation time of total alkali and combined anthraquinone proportion compatibility treated animal and the 3h has respectively significantly or utmost point significant difference, and detailed results sees Table 3.
Table 3 total alkali and combined anthraquinone compatibility are to the influence of normal rat defecation
| Group | n | Dosage/mlkg -1 | Body weight | First grain defecation time/min | Defecation weight/g in the 3h |
| Blank group total alkali group combined anthraquinone group total alkali: combined anthraquinone (2: 1) total alkali: combined anthraquinone (1: 1) total alkali: combined anthraquinone (1: 2) | 666666 | 10.0 5.0 10.0 7.5 10.0 15.0 | 205.33±6.53 202.50±5.68 199.83±3.43 198.83±7.86 200.83±4.79 199.50±5.54 | 77.17±39.60 67.17±44.29 33.50±10.84 1) 25.50±8.78 1) 30.83±7.89 1) 35.17±13.29 1) | 1.79±0.72 2.47±0.76 3.29±1.30 1) 4.63±0.92 2)4) 4.24±1.02 2)4) 3.59±0.64 1)3) |
Compare with the blank group
1)P＜0.05,
2=P＜0.001.Ratio group and total alkali group are relatively
3=P＜0.05,
4=P＜0.01.
4 experiment conclusion
The experimental result prompting, list has the trend that shortens animal first grain defecation time and increase the animal feces volume with total alkali, and list can obviously shorten animal first grain defecation time and promote the animal feces volume with combined anthraquinone; Total alkali and combined anthraquinone proportion compatibility group all have significant shortening animal first grain defecation time and promote the animal defecation, and 3 proportion compatibility treated animal defecation effects are better than single with total alkali or combined anthraquinone.Experimental result is also pointed out, and total alkali and general anthraquinone compatibility have certain synergistic function to intact animal's defecation, and the first grain of animal defecation time and shorten with the increase of total alkali ratio, and the gross weight that voids excreta in the animal 3h increases weight with the increase of total alkali ratio.
Total alkali, combined anthraquinone, total alkali and 3 proportion compatibilities of combined anthraquinone promote the strong and weak order of animal defecation to be followed successively by total alkali and 2: 1＞total alkali of combined anthraquinone compatibility and 1: 1＞total alkali of combined anthraquinone compatibility and 1: 2＞combined anthraquinone＞total alkali of combined anthraquinone compatibility.
The different proportion compatibilities with rhubarb-combined anthraquinone of test example 4 Radix Aconiti Lateralis Preparata total alkalis are to the influence of yang deficiency constipation rat model defecation
1 experiment material
1.1 experiment medicine
Be subjected to the reagent thing, the Radix Aconiti Lateralis Preparata total alkali, every 1ml contains the 1kg crude drug, and the every 1ml of combined anthraquinone contains the 1.2g crude drug.Before being made into 1: 1,1: 2,2: 1 medicinal liquids, total alkali becomes every 1ml to be equivalent to the 1.0g crude drug with the buffer of pH6.8 respectively with total quinone with distilled water diluting.
The modeling medicine, the Shanxi white vinegar, total acidity＞4.5g/100ml is produced by Shanxi mature vinegar factory.Active carbon, east, Chongqing examination chemical industry company limited is produced.Ice is from freezing.
1.2 laboratory animal
The healthy adult SD rat, body weight 200～220g.Laboratory animal production licence number: 04-11.Provide by Chengdu University of Traditional Chinese Medicine animal experiment study center, the quarantine back is standby.
2 experimental techniques
Get 42 of healthy adult SD rats, be divided into blank group (N=6), model control group (N=6), total alkali group (N=6), combined anthraquinone group (N=6), total alkali at random: combined anthraquinone (2: 1) group (N=6), total alkali: combined anthraquinone (1: 1) group (N=6), total alkali: combined anthraquinone (1: 2) group (N=6) 7 groups.Except that the blank group, other each treated animals are all irritated stomach by first dosage 20ml/kg and are given Shanxi white vinegar and 0 ℃ of active carbon frozen water, twice modeling administration time is at a distance of 4h, continuous modeling 9d, modeling simultaneously, each treated animal is pressed 10ml/kg every day respectively, 10ml/kg, 5.0ml/kg, 10ml/kg, 7.5ml/kg, 10ml/kg, 15ml/kg irritates the stomach normal saline, normal saline, total alkali, combined anthraquinone, total alkali: combined anthraquinone=2: 1,1: 1,1: 2 medicinal liquid, continuous 9d, weigh, after the last administration, immediately rat is pursued and only put into metabolic cage, place mat A4 copy paper under the metabolic cage, drinking water supply is observed the gross weight that voids excreta in its first grain time that voids excreta and its 3h that weighs, and is write down just matter.Test data is handled with the SPSS11.0 statistical software.
3 experimental results
The molding of blank treated animal feces, oblong, yellow skin, yellowish green, light brown or black.Model group animal row black, molding, slightly hard dried matter feces.The administration treated animal is all lined up type graininess Melaena.Compare with the blank group: the first grain of model group animal defecation time obviously postpones, and the gross weight that voids excreta in the 3h is less than the blank group, but not statistically significant.Compare with model control group: total alkali group, combined anthraquinone group, total alkali: the gross weight that voids excreta in the first grain defecation time of each ratio treated animal of combined anthraquinone and the 3h has respectively significantly or utmost point significant difference.With single with the total alkali group relatively: total alkali: the gross weight that voids excreta in the 3h of combined anthraquinone=2: 1 and 1: 1 treated animal has significant difference, and total alkali: the gross weight that voids excreta in the 3h of combined anthraquinone=1: 2 treated animal also has increase trend, and detailed results sees Table 4.
Table 4 total alkali and combined anthraquinone compatibility are to the influence of yang deficiency constipation rat model defecation
| Group | n | Dosage/mlkg -1 | Body weight before the experiment | Experiment back body weight | First grain defecation time/min | Defecation weight/g in the 3h |
| Blank group model group total alkali group combined anthraquinone group total alkali: in conjunction with quinone (2: 1) total alkali: in conjunction with quinone (1: 1) total alkali: in conjunction with quinone (1: 2) | 6666666 | 10.010.05.010.07.510.015.0 | ?209.50±4.51?210.17±3.43?209.83±2.64?210.17±4.17?209.50±2.43?209.00±3.22?210.00±2.90 | 221.50±3.94 216.50±4.51 214.33±6.56 217.00±3.74 220.17±4.88 220.67±4.76 211.33±4.41 | 69.33±39.64 146.50±50.16 1) 77.67±53.60 2) 36.50±4.97 3) 45.17±18.80 3) 37.33±21.41 3) 37.83±16.07 3) | 1.57±0.21 0.93±1.03 1.31±0.65 2.02±0.15 2) 2.40±0.95 2)4) 3.16±1.76 2)4) 1.84±0.31 2) |
Compare with the blank group
1)P＜0.05.Compare with model group
2)P＜0.05,
3)P＜0.01.Proportion compatibility group and total alkali group are relatively
4=P＜0.05.
4 experiment conclusion
The experimental result prompting, single have the first grain of obvious shortening animal pattern defecation time and the trend that increases the gross weight that voids excreta in the animal pattern 3h with total alkali.Single have obvious shortening animal pattern first grain defecation time and significantly increase the gross weight effect that voids excreta in the animal pattern 3h with combined anthraquinone.1: 1,2: 1,1: 2 ratio of total alkali and combined anthraquinone compatibility all has remarkable shortening animal first grain defecation time and obviously increases the gross weight effect that voids excreta in the animal pattern 3h.Experimental result is also pointed out, total alkali and combined anthraquinone compatibility 1: 1 and 2: 1 ratios with the total alkali group relatively have certain synergistic function to the defecation of animal pattern with single, but, each proportion compatibility of total alkali and combined anthraquinone compares with the combined anthraquinone group with single, there is no synergistic function.
Total alkali: 1: 1,2: 1,1: 2 ratio group promotion animal pattern defecation intensity that conjunction type is feared the quinone compatibility is better than list and uses the total alkali group, and total alkali: each proportion compatibility group of general anthraquinone is not seen notable difference with single with the combined anthraquinone group to the influence of animal pattern defecation.
Claims (6)
1. pharmaceutical composition for the treatment of constipation, it is characterized in that: it is the medicament that is prepared from by following raw materials by weight proportions:
Radix Aconiti Lateralis Preparata total alkaloids: total Radix Et Rhizoma Rhei anthraquinone=1: 2-1: 1.
2. the pharmaceutical composition of treatment constipation according to claim 1 is characterized in that: described Radix Aconiti Lateralis Preparata total alkaloids, in aconitine content 〉=30%w/w; Total Radix Et Rhizoma Rhei anthraquinone is with 1,8 dihydroxyanthraquinone meter content 〉=40%w/w.
3. pharmaceutical composition for the treatment of constipation is characterized in that: it is the medicament that the crude drug by the following weight compatibility is prepared from:
Radix Aconiti Lateralis Preparata total alkaloids: rhubarb-combined anthraquinone=2: 1-1: 1; Described rhubarb-combined anthraquinone is with 1,8 dihydroxyanthraquinone meter content 〉=30%w/w.
4. the pharmaceutical composition of treatment constipation according to claim 1 is characterized in that: described medicament is tablet, granule, capsule, pill or oral liquid.
5. a method for preparing the pharmaceutical composition of the described treatment constipation of claim 1 comprises the steps:
A, take by weighing the materials of weight proportions medicine:
Radix Aconiti Lateralis Preparata total alkaloids: total Radix Et Rhizoma Rhei anthraquinone=1: 2-1: 1;
B, mixing add acceptable accessories or complementary composition and are prepared into preparation pharmaceutically commonly used.
6. the purposes of the described pharmaceutical composition of claim 1 in the medicine of preparation treatment constipation.
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