CN101182564A - Function aquatic peptide capable of promoting growth of aquatic animals and enhancing disease resistance and method of producing the same - Google Patents
Function aquatic peptide capable of promoting growth of aquatic animals and enhancing disease resistance and method of producing the same Download PDFInfo
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- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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Abstract
The invention relates to a functional aquatic peptide for promoting the growth and the disease resistance of aquatic animals and a preparation method hereof; with the method, aquatic products such as fish and shrimp are taken as the raw materials for processing byproducts; the aquatic products are chopped by a chopper and then ground by a colloid grinder into 50 to 100 superfine colloid serum; and then the added endonuclease and the endonuclease of the aquatic product raw materials form compound hydrolyzation and for fungus fermentation and preparation of the functional aquatic peptide. The method of the invention solves the problems of waste of biological resources and environmental pollution; the product made with the invention can be used to be evenly mixed with feed in a pattern of progressive enlargement for making grain feed; the feed is provided with the capability of promoting the growth and disease resistance of the aquatic animals and at the same time the feed can improve the water quality and increase the rate of gain and survival rate.
Description
(1) technical field: the biotechnology that the present invention relates to aquatic products processing by products such as fresh fish, shrimp utilizes the field, is specifically related to get everything ready short aquatic animal to grow and improve the method for the function aquatic peptide of disease resistance of applying biological enzymolysis combining with fermentation legal system.
(2) background technology: along with the development of society, people increase day by day to the demand of non-harmful product, therefore for preventing that drug residue from producing harm to the mankind, Food and Argriculture OrganizationFAO, International Animal Health tissue and the World Health Organization propose to control the use of animal pharmaceuticals jointly, will thoroughly forbid the chemical substances such as somatotropin of animal use microbiotic and synthetic future.Thereby, novel pollution-free, noresidue, nuisanceless, the safe green fodder additives that can improve animal immunizing power, disease resistance and production performances such as poultry, fowl, aquatic products will become the emphasis of feedstuff industry innovation and research and development.
Peptide is the not exclusively product of degraded of protein, and its nutritive value and physiological function in vivo is extremely important, therefore is called as biologically active peptides.Bioactive peptide comprises the endogenous bioactive peptide (if any the enkephalin of analgesic activity, the antiviral Zadaxin of improving the liver function effect etc.) that exists naturally in the organism, and natural protein is through the exogenous bioactive peptide (as phosphopeptide caseinate, soybean blood pressure lowering peptide, aquatic products blood pressure lowering peptide etc.) of enzymic hydrolysis generation.Because the oligopeptides that enzymolysis makes has the different physiological roles effect, thereby is widely used in fields such as medicine, health care, livestock and poultry, demonstrates good prospects for application.
The biological function peptide is paid close attention to by various countries scientist and entrepreneur gradually as a kind of green feed additive.The U.S. is happy company's China Branch to be raw material with the soybean, adopt biological enzyme to prepare soybean peptides, name of product is happy energy soybean peptides, and the evidence product has pig, fowl Digestive tract, the immunity system of adjusting, improves intestinal environment, improves efficiency of feed utilization and disease resistance.Hua Zhong Agriculture University's school of life and health sciences, national feed Engineering Technical Research Centre, Jiangxi Province Academy of Agricultural Sciences successively study and adopt mixed solid fermentation technological development soybean protein peptide, the soybean protein peptide external digestion rate that obtains is greater than 95%, can be used for preparing the nutritional additive of the feed such as piglet, calf of early weaning, become the protein fodder of easily digesting and assimilating.In recent years (ZL200410029787.0) to propose with vegetable-protein, animal proteinum be raw material to a patent " a kind of activated peptide forage additive and its production and use ", microbe fermentation method degraded plant animal proteins such as employing genus bacillus prepare bioactive peptide, this bioactive peptide is added into the weanling pig feed, and piglet day weight gain rate and feed conversion rate are significantly higher than blank group and microbiotic group.Bioactive peptide makes an addition to layer diets, can improve laying rate, increases egg size, reduce the feed intake rate, and the influence of pair chicken peripheral blood-T lymphopoiesis vigor is arranged simultaneously.
Yet activated peptide forage additive all is confined to tame livestock and poultry at present, serves as the raw material that bioactive peptide is produced in preparation with land protein source such as soybean, animal skin and internal organ substantially also.
Aquatic animal is a poikilothermal animal because living environment and tame livestock and poultry are completely different, and is low etc. than livestock and poultry on evolutionary history, and the special physiological properties characteristic is arranged.The structure of bioactive peptide that the specificity of biologically active peptides has determined to regulate the aquatic animal physiological function is with tame livestock and poultry different.Its special aquifer cultivation environment also brings certain difficulty for trial-production functionally active peptide, therefore has the functional peptides product of regulating aquatic animal physiological function characteristic and has not also seen have.
Aquatic products fish, shrimps deep processing will produce the tankage about 50%, and stock number is big.Contain in the tankage protein up to 48%, essential amino acids content accounts for more than 50% of total amino acid, contains extremely abundant Ca, P, constant and trace elements such as Fe, Se.Shrimp head, fish body internal organ contain a large amount of biological enzymes and unknown somatomedin thereof, being rich in the indispensable amino acid and the die aromatischen Aminosaeuren that have the short aquatic animal to grow of preparation adjusting and improve physiological functions such as disease resistance simultaneously, is the good raw material of preparation aquatic products bioactive peptide.These processing fent parts are used to produce roughage at present, because processing means is original, feed granules is thick, and fish protein and fish-bone can not fully be utilized by aquatic animal.The processing fent that has causes rotting to be dropped because of can not get processing in time.At present shrimp head capsule tankage are mainly used in the production chitin and since the strong acid and strong base production technique havoc of chitin nutritive substance structures such as protein, astacin in the shrimp tankage, lost proteinic nutritive value.Valuable Biological resources become waste, cause the wasting of resources and environmental pollution.
The living environment of aquatic animal is water body, because water temperature is low, be again poikilothermal animal, so digestive gland is undeveloped, and digestive tube is simple, and digestive ferment causes raw material availabilities such as conventional aquatic feeds starch, protein very low because of the not high factor of body temperature low activity.The present invention is a raw material with the aquatic products processing by product, adopt advanced pretreatment technology, reach the biotechnology that compound biological enzyme and the submerged fermentation of many bacterial classifications of microorganism aerobic combine, the function aquatic peptide of preparation has the function that improves intravital proteinase activity of aquatic animal and amylase activity, the absorption that promotes aquatic animal calcium and phosphorus is arranged simultaneously, improve the body microenvironment, improve the effect of immunizing power and disease resistance.Improve the utilization ratio of protein and starch in the feed greatly, improve aquatic animal to grow and surviving rate.
(3) summary of the invention: task of the present invention provides a kind of method that adopts the biotechnology processing function aquatic peptide that advanced pretreatment technology, compound biological enzyme and the submerged fermentation of many bacterial classifications of microorganism aerobic combine, the function aquatic peptide that this method is produced can be used as feeding additive aquatic animal, have the aquatic animal to grow of promotion and disease resistance enhancemen, improve the aquaculture product quality simultaneously, improve the rate of body weight gain and the surviving rate of culturing aquatic products.
Aquatic products processing by products such as fresh fish, shrimp are ground to form 80～100 purpose colloid things through colloidal mill, again fine colloid slurry is transferred pH to 5.0～8.5, be combined to form the composite hydrolysis toolenzyme with original biological enzyme in 0.2%～0.4% restriction endonuclease of raw material and the raw material, composite hydrolysis 1.5～2 hours, hydrolysis temperature is controlled at 30～45 ℃, makes being hydrolyzed into of protein macromolecule big scale be big peptide chain molecule; In the liquid raw material of first one-step hydrolysis, add the fermentation media carbohydrate, trace element etc., transfer pH to 6.0～7.5, after the sterilization, insert fungi A.S 3.042, A.S 3.350, starting temperature is controlled at 30～32 ℃, ferment after 10 hours, heat of fermentation heats up reaction system, treats that the fermentation system temperature near 35 ℃, begins to feed aseptic gas, the control temperature of reaction system is at 32～35 ℃, this moment, microorganism obtained competent oxygen, growth fast, and secrete neutral protease in a large number, aspartic protease, Collagenases etc. form prozyme system, make raw material, microorganism, proteolytic enzyme is a system altogether, carries out submerged fermentation.The macromole peptide is become the small-molecular peptides that promotes growth is arranged and regulate immunologic function by the further directionally hydrolyzing of the proteolytic enzyme of microorganism secretion in this system.The fermentation later stage, owing to be subjected to the regulation and control of acidophilia microorganism, and the decomposition of fermented materials such as protein and sugar makes fermentation system be acid, fish-bone, fish scale, the calcareous beginning of crust are decomposed at this moment, be converted into and easily be the ionized calcium of animal body absorption, make that fish-bone, fish scale, crust are calcareous to obtain abundant trans-utilization.Fermented 30～48 hours, through sterilization, concentrate, fluidised bed drying, make powder product, or the product behind the fermentation and sterilization made 25% concentrated product that contains solid thing through concentrating.
Technical scheme of the present invention:
Short aquatic animal to grow provided by the invention and improve the function aquatic peptide of disease resistance is to utilize fresh fish, shrimp aquatic products processing by product to be raw material, the function aquatic peptide that makes through following procedure of processing.
Its preparation method comprises the steps:
(1) be raw material with fresh fish, shrimp aquatic products processing by product, become thick rotten slurry through the mincer rubbing, rotten slurry adds water, and 1 part of slightly rotten slurry adds 2～4 parts of water, wear into through colloidal mill again and be (50～100 order) fine colloid slurries, for next step biological enzyme hydrolysis provides more effect substrate; The present invention is a raw material with aquatic products processing by products such as fresh shrimp head capsule, fishes directly, has kept original endogenous biological enzyme and trace element, VITAMIN, somatomedin etc. nutritive element in shrimp internal organ and the fish body;
(2) with above-mentioned fine colloid slurries adjust pH to 5.0～8.5, press the weight ratio of slurries raw material, the restriction endonuclease of adding 0.2%～0.4%, should be combined to form the composite hydrolysis toolenzyme with original endogenous biological enzyme in the raw material, carried out enzymic hydrolysis 1.5～2 hours, 30～45 ℃ of hydrolysis temperatures make being hydrolyzed into to big peptide chain molecule of protein macromolecule big scale, obtain enzymolysis solution;
(3) above-mentioned enzyme hydrolyzate is fermented:
A. prepare fermention medium: substratum is a liquid, each component ratio: peptone 1.0～2.5g, glucose 4～5g, Zulkovsky starch 10～20g, sucrose 10～15g, analysis for soybean powder 5～15g, maltose 2～5g, somatomedin 3g, tap water 1000ml, wherein somatomedin is Na
2PO
42g, Mg
2SO
4The mixture of 1g is regulated pH to 6.0～7.5, and sterilization obtains liquid nutrient medium;
B. prepare seed liquor: fungi A.S 3.042 and A.S 3.350 are inserted in the aforesaid liquid substratum after the sterilization simultaneously, cultivated 48～72 hours in 28～32 ℃, obtain seed liquor;
C. zymotechnique: above-mentioned enzymolysis solution is added in the fermentor tank, add carbon source then, making the ratio of nitrogen content and the weight of the carbon content that adds carbon source in the enzymolysis solution is 2～10: 1, transfer pH to 6.0～7.5, to treat fermented liquid 0.12～0.15MPa, 121～125 ℃ the sterilization 20～30 minutes, be cooled to 25～35 ℃; Add seed liquor, the volume ratio of seed liquor and fermented liquid is 0.3～1.5: 10, the fermentation starting temperature is controlled at 30～32 ℃, behind the prior fermentation 10～18 hours, heat of fermentation heats up reaction system, treats that the fermentation system temperature is near 35 ℃, begin to feed sterile air, make fermentation system obtain competent oxygen, carried out the aerobic submerged fermentation then 20～30 hours, the control temperature of reaction system is at 32～35 ℃; This moment, microorganism obtained competent oxygen, growth fast, and secrete neutral protease, aspartic protease, Collagenase etc. in a large number and form prozymes system, making raw material macromole peptide, microorganism, proteolytic enzyme is a system altogether, and the macromole peptide is become the small-molecular peptides that promotes growth is arranged and regulate immunologic function in this system by the further directionally hydrolyzing of the proteolytic enzyme of microorganism secretion; The fermentation later stage, owing to be subjected to the regulation and control of acidophilia microorganism, and the decomposition of fermented materials such as protein and sugar makes fermentation system be acid, fish-bone, fish scale, the calcareous beginning of crust are decomposed, be converted into and easily be the ionized calcium of animal body absorption, make that fish-bone, fish scale, crust are calcareous to obtain abundant trans-utilization; Overall fermentation time was controlled at 30～48 hours, will produce nutritive elements such as more function aquatic products small peptide, biological calcium ion;
(4) above-mentioned fermented liquid is made powder product through sterilization, concentrated, fluidised bed drying; Or with fermented liquid sterilization after concentrate and make 25% concentrated solution that contains solid thing.
Described (2) the specificity restriction endonuclease that adds of step is a kind of in trypsinase, Quimotrase, stomach en-, the bacillus subtilis neutral proteinase, and add-on is 0.2%～0.4% of an enzymolysis raw material.
In described (3), the inoculum size of fungi A.S 3.042 and A.S 3.350 is 1.0 * 10
5The cfu/ml substratum, the fungi A.S 3.042 of access and the ratio of A.S 3.350 10: 2.
Described trypsinase, Quimotrase are available from Hangzhou three leaf biological enzyme formulation factories, vigor 3000U/g, optimal pH 7.5～8.5,40 ℃ of enzymolysis optimum temperutures; Stomach en-is available from Shanghai biochemical reagents factory, vigor 4500U/g, optimal pH 4～5,38 ℃ of enzymolysis optimum temperutures; Bacillus subtilis neutral proteinase, available from Wuxi zymin factory, vigor 50000U/g, 42 ℃ of optimum temperutures, optimal pH 7.2.
Described fungi A.S 3.042, A.S3.350 are foodstuffs industry and use bacterium, and edible safety is all available from Microbe Inst., Chinese Academy of Sciences.A.S 3.042 is an aerobic organism, fast growth, conidium is big, quantity is many, strong to the resistibility of assorted bacterium, its optimum growth temperature is 30～33 ℃, the highest should not be above 38 ℃, optimal pH 7.5, cultivate 20 hours for producing the enzyme animated period, 28 hours is the peak period, mainly secretes neutral protease, also has polygalacturonase, amylase etc.28～30 ℃ of A.S 3.350 optimum growth temperatures, optimal pH 6.5 is cultivated 24 hours for producing the enzyme animated period, and 30 hours is the peak period, main secreting acidic proteolytic enzyme, saccharifying enzyme, polygalacturonase and a small amount of peptide ending enzyme etc.
The present invention adopts sodium hydroxide or hydrochloric acid impregnation somaplasm pH value, medium pH value and fermentation cylinder for fermentation liquid pH value.
The advance of the technology of the present invention is:
1) this method is used in combination bio-enzyme engineering, the submerged fermentation of many bacterial classifications of microorganism in the degree of depth of aquatic products processing by product and transforms, make protein transduction in the low value resource of waste originally turn to short aquatic animal to grow, improve the small-molecular peptides of immunologic function, realize that fish-bone, fish scale are converted into biological calcium, make resource obtain fully utilizing.
2) liquid raw material of preliminary enzymolysis (or the gruel of Mechanical Crushing slurry) can be decomposed by the multiple protein enzyme of microorganism secretion by the collaborative submerged fermentation of multiple microorganism, improves function small-molecular peptides ratio greatly.
3) the fermentation middle and later periods, make fermentation system be acid owing to be subjected to the regulation and control of acidophilia microorganism and the decomposition of fermented materials such as protein and sugar, activated the activity of aspartic protease, Collagenase effectively, make fish-bone, fish scale, crust is calcareous begins to be decomposed in biological enzyme and sour environment, is converted into easily the ionized calcium for the animal absorption;
4) technology and technology reach resource all conversion and utilization, and no waste discharge has been guaranteed ecological environment security.
Adopt high performance liquid chromatography [method reference: Chinese food journal, 2006,6 (3): 128～132, exercise question: phosphopeptide caseinate in the high effective liquid chromatography for measuring milk powder (CPP) content] analyze and to record in the product of the present invention peptide relative molecular mass distribute (table 1), wherein the small peptide content of relative molecular mass below 5000 is up to more than 95%, and the peptide with active function generally is the small molecules small peptide.
| Relative molecular mass Relative molecular mass (u) | Ratio Ratio (%) | Peptide chain length (amino acid no) Length of peptide chain |
| 13800 8650 7850 6500 5430 4890 4320 3860 2680 1890 1550 ＜1000 | 0.32 0.25 0.92 0.48 2.86 1.98 0.95 11.99 12.02 5.92 10.12 52.16 | 108 68 61 51 42 38 34 30 21 15 12 ＜8 |
Add the functional peptides pulvis of the present invention of 1%-3% in aquaculture feed, employing enlarges the blended method step by step and feed mixes, and makes granulated feed and feeds; Maybe will ferment after concentrate 25% concentrated solution that contains solid thing make directly spraying make an addition in the conventional feed and feed; Culture 45 days (under the condition of feeding on an equal basis, being contrast), record the trypsinase vigor and improve 45%-53% not add product of the present invention, amylase activity improves 50%-65%, rate of body weight gain improves 10%-15%, and surviving rate improves 12%-15%, and function is remarkable.
(4) specific embodiments:
Embodiment 1:
(1) with 100 kilograms on fresh shrimp head, shell, become thick gruel slurry through the mincer rubbing, thick rotten the slurry adds the water mixing, and the add-on of water is that 1 part of thick rotten slurry adds 2～4 parts of water, and present embodiment is 4 parts of water, becomes 50～100 purpose colloid slurries through colloidal mill again;
(2) the colloid slurries are transferred pH to 8.5,0.2% trypsin or the Quimotrase that add colloid slurries raw material weight), and with shrimp head (shell) in a large amount of endogenous enzyme (original endogenous biological enzyme has lipase, liver pancreas proteolytic enzyme, liver pancreas amylase, entero-amylase, chitinase etc.) compound, common hydrolysis 2 hours, 42 ℃ of hydrolysis temperatures, protein macromolecule in the shrimp head capsule is hydrolyzed into to big peptide chain molecule, makes hydrolyzed solution;
(3) preparation seed liquor:
The preparation substratum: peptone 1.0～2.5g, glucose 4～5g, Zulkovsky starch 10～20g, sucrose 10～15g, analysis for soybean powder 5～15g, maltose 2～5g, somatomedin 3g, tap water 1000ml, wherein somatomedin is Na
2PO
42g, Mg
2SO
4The mixture of 1g is regulated pH to 6.0～7.5, and sterilization obtains liquid nutrient medium;
Fungi A.S 3.042 and A.S 3.350 are inserted in the liquid nutrient medium after the sterilization simultaneously, and inoculum size is 1.0 * 10
5The cfu/ml substratum, the fungi A.S 3.042 of access and the ratio of A.S 3.350 10: 2, in 28～32 ℃ of cultivations 48～72 hours, present embodiment was cultivated 60 hours, obtained seed liquor;
(4) fermentation: the enzymolysis solution that step (2) is obtained adds in the fermentor tank, measure the nitrogen content of enzymolysis solution, adding carbon source flour (or Semen Maydis powder, or broken rice meal, or sweet potato flour) and sucrose, both weight ratios=4: 1, the add-on of carbon source is that to make the carbon-nitrogen ratio of fermented liq be 2～10: 1, and present embodiment is 10: 1, transfers pH to 7.2, to treat again fermented liquid 0.12～0.15MPa, 121～125 ℃ the sterilization 20～30 minutes, be cooled to 25～35 ℃; Add seed liquor, the volume ratio of seed liquor and fermented liquid is 0.3～1.5: 10, the fermentation starting temperature is controlled at 30～32 ℃, ferment after 10～18 hours, begin to feed sterile air, Ventilation Rate is 1.5L/min, make fermentation system obtain competent oxygen, microorganism entered the aerobic submerged fermentation 20～30 hours, and the control temperature of reaction system produces more function aquatic products small peptide at 32～35 ℃.
(5) fermented liquid is made 30 kilograms of products of the present invention (for the shrimp powder product) through sterilization, concentrated, fluidised bed drying.
(6) application of product of the present invention:
Add 2% the invention described above product (pulvis) in the prawn culturing feed, employing enlarges the blended method step by step and feed mixes, and makes granulated feed and feeds.
Embodiment 2:
(1) fresh fish processing byproduct (minced fish, pork skin, fish-bone, fish scale, internal organ etc.) is 100 kilograms, and rubbing through mincer becomes thick rotten slurry, and rotten slurry adds water (+2 parts of water of 1 part of thick rotten slurry), becomes the fine colloid slurries of 80 orders through colloidal mill again;
(2) the colloid slurries are transferred pH to 7.0,0.4% bacillus subtilis neutral proteinase (or the stomach en-that adds colloid slurries raw material weight, this moment, slurries were transferred pH to 5.0), and it is compound with the endogenous enzyme (endogenous biological enzyme has lipase, liver pancreas proteolytic enzyme, liver pancreas amylase, entero-amylase, chitinase etc.) of fish viscera, common hydrolysis 2 hours, 45 ℃ of hydrolysis temperatures make that protein macromolecule is hydrolyzed into to big peptide chain molecule in the tankage, make hydrolyzed solution;
(3) preparation seed liquor: with embodiment 1;
(4) fermentation: the enzymolysis solution that obtains in the step (2) is added in the fermentor tank, measure the nitrogen content of enzymolysis solution, add carbon source flour and sucrose (weight ratio of flour and sucrose=4: 1) then, the add-on of carbon source is that to make the carbon-nitrogen ratio of fermented liq be 5: 1, transfer pH to 7.2, to treat again fermented liquid 0.12～0.15MPa, 121～125 ℃ the sterilization 20～30 minutes, be cooled to 25～35 ℃; Add seed liquor, the volume ratio of seed liquor and fermented liquid is 1: 10, the fermentation starting temperature is controlled at 30～32 ℃, ferment after 12 hours, begin to feed sterile air, Ventilation Rate is 1.5L/min, make fermentation system obtain competent oxygen, microorganism entered the aerobic submerged fermentation 30 hours, and the control temperature of reaction system produces more function aquatic products small peptide at 32～35 ℃;
(5) fermented liquid is made 25 kilograms of products of the present invention through sterilization, concentrated, fluidised bed drying.
(6) application of product of the present invention:
Add 3% the invention described above product (pulvis) in the prawn culturing feed, employing enlarges the blended method step by step and feed mixes, and makes granulated feed and feeds.
Claims (5)
1. a short aquatic animal to grow and improve the function aquatic peptide of disease resistance is characterized in that utilizing fresh fish, shrimp aquatic products processing by product to be raw material, makes function aquatic peptide through following procedure of processing, and its preparation method comprises the steps:
(1) be raw material with fresh fish, shrimp aquatic products processing by product, rubbing through mincer becomes thick rotten slurry, and rotten slurry adds water, and 1 part of thick rotten slurry adds 2～4 parts of water, and wearing into through colloidal mill is the fine colloid slurries of 50～100 purposes again;
(2) with above-mentioned fine colloid slurries adjust pH to 5.0～8.5, the weight ratio of pressing the slurries raw material, the restriction endonuclease of adding 0.2%～0.4%, be combined to form the composite tool enzyme with original endogenous biological enzyme in the raw material, carried out enzymic hydrolysis 2 hours, 30～45 ℃ of hydrolysis temperatures obtain enzymolysis solution;
(3) above-mentioned enzyme hydrolyzate is fermented,
A. prepare fermention medium: substratum is a liquid, each component ratio: peptone 1.0～2.5g, glucose 4～5g, Zulkovsky starch 10～20g, sucrose 10～15g, analysis for soybean powder 5～15g, maltose 2～5g, somatomedin 3g, tap water 1000ml, wherein somatomedin is Na
2PO
42g, Mg
2SO
4The mixture of 1g is regulated pH to 6.0～7.5, and sterilization obtains liquid nutrient medium;
B. prepare seed liquor: fungi A.S 3.042 and A.S 3.350 are inserted in the aforesaid liquid substratum after the sterilization simultaneously, cultivated 48～72 hours in 28～32 ℃, obtain seed liquor;
C. zymotechnique: above-mentioned enzymolysis solution is added in the fermentor tank, add carbon source then, making the ratio of nitrogen content and the weight of the carbon content that adds carbon source in the enzymolysis solution is 2～10: 1, transfer pH to 6.0～7.5, to treat fermented liquid 0.12～0.15MPa, 121～125 ℃ the sterilization 20～30 minutes, be cooled to 25～35 ℃; Add seed liquor, the volume ratio of seed liquor and fermented liquid is 0.3～1.5: 10, the fermentation starting temperature is controlled at 30～32 ℃, ferments after 10～18 hours, and heat of fermentation heats up reaction system, treat that the fermentation system temperature is near 35 ℃, begin to feed sterile air, make fermentation system obtain competent oxygen, the control temperature of reaction system is at 32～35 ℃, microorganism entered the aerobic submerged fermentation 20～30 hours, produced more function aquatic products small peptide;
(4) above-mentioned fermented liquid is made product through sterilization, concentrated, fluidised bed drying.
2. the preparation method of function aquatic peptide according to claim 1 is characterized in that described restriction endonuclease is a kind of proteolytic enzyme in trypsinase, Quimotrase, stomach en-, the bacillus subtilis neutral proteinase.
3. the preparation method of function aquatic peptide according to claim 1 is characterized in that original endogenous biological enzyme has lipase, liver pancreas proteolytic enzyme, liver pancreas amylase, entero-amylase, chitinase in the fish, shrimp aquatic products processing by product of described raw material.
4. the preparation method of function aquatic peptide according to claim 1 is characterized in that the carbon source that adds in the fermentor tank is a flour, or Semen Maydis powder, or broken rice meal, or sweet potato flour.
5. the preparation method of function aquatic peptide according to claim 1 is characterized in that with the inoculum size in fungi A.S 3.042 and the A.S 3.350 access liquid nutrient mediums be 1.0 * 10
5The cfu/ml substratum, the ratio of fungi A.S 3.042 and A.S 3.350 10: 2.
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