CN101181632B - Method for preparing superoxide dismutase microcapsule - Google Patents
Method for preparing superoxide dismutase microcapsule Download PDFInfo
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- CN101181632B CN101181632B CN200710177979XA CN200710177979A CN101181632B CN 101181632 B CN101181632 B CN 101181632B CN 200710177979X A CN200710177979X A CN 200710177979XA CN 200710177979 A CN200710177979 A CN 200710177979A CN 101181632 B CN101181632 B CN 101181632B
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Abstract
The invention relates to a superoxide dismutase microcapsule preparing method, pertaining to the field of biological chemical product. The stability of the natural superoxide dismutase is poor and the application is restricted. The invention has the following steps: crushing and sieving the ethide fibrin and the bovine colostrum; adding 60 to 120 meshes of ethide fibrin into the methylene dichloride and then stirring and dissolving so as to get mixed liquor A; mixing 80 to 120 meshes of bovine colostrum and superoxide dismutase according the mass ratio from 1:4 to 4:1; adding the mixture of the bovine colostrum and the superoxide dismutase or the pure superoxide dismutase into A, then mixing to complete swelling so as to get mixed liquor B which is put stilly; the mass ratio between the mixture of the bovine colostrum and the superoxide dismutase, or the pure superoxide dismutase and the ethide fibrin is 3:1 to 5:1; dissolving the dodecyl sodium sulfate into distilled water, stirring to clear liquor so as to get liquor C; adding the B into C for stirring so as to ensure the methylene dichloride to be completely volatilized; decompressing and filtering the distilled water, ventilating and drying. The invention improves the stability of the superoxide dismutase and the biological utilization degree as well as the processing performance.
Description
Technical field
The microencapsulation of superoxide dismutase, product are suitable for fields such as Medicines, health food and biochemical industry product.
Background technology
Superoxide dismutase is a kind of important oxygen free radical scavenger in the body, oxygen-derived free radicals that can the balance body, thus avoid the untoward reaction that causes during the ultra-oxygen anion free radical excessive concentration when in the body.Superoxide dismutase is combined with microcapsule technology, make the superoxide dismutase microcapsule and do not see the pertinent literature report at home and abroad as yet.At present, because natural superoxide dismutase poor stability is restricted its application surface.
Superoxide dismutase is made the microcapsule product, both improved the stability of superoxide dismutase, the factors such as air, moisture, light that prevent cause the variation of active component, be beneficial to absorption of human body, can reduce the superoxide dismutase product again directly stimulates gastrointestinal, be more convenient for further making and be convenient to transport, have medicine, food or the biochemical industry product etc. of health care.
Summary of the invention
The present invention has realized the microencapsulation of superoxide dismutase, thereby has improved the stability and effective bioavailability of superoxide dismutase, and has improved the processing characteristics of product.The present invention first with distilled water as reaction system, the microcapsule of preparing all meets the commercialization requirement from adhesion situation, smooth surface degree between roundness, particle size distribution, granule.
The invention provides a kind of preparation method of microencapsulation of superoxide dismutase, it is characterized in that, may further comprise the steps:
(1) ethyl cellulose and cattle colostrums are pulverized, sieved respectively; Measure dichloromethane; After sieving 60 joined in the dichloromethane to 120 purpose ethyl celluloses, and the limit edged stirs, and it is dissolved fully, obtains mixed liquor A;
(2) 80 to 120 purpose cattle colostrumss after will pulverizing and superoxide dismutase mix by 1: 4 to 4: 1 mass ratio, obtain the two mixture; With cattle colostrums and superoxide dismutase mixture, perhaps pure superoxide dismutase joins mixed liquor A, and the limit edged stirs, and to dissolving fully, obtains mixed liquid B, leaves standstill; Cattle colostrums and superoxide dismutase mixture, the mass ratio of perhaps pure superoxide dismutase and ethyl cellulose is 3: 1 to 5: 1;
(3) dodecyl sodium sulfate is dissolved in the distilled water, is stirred to the solution clarification, obtain solution C, the water-bath temperature control stirs;
(4) mixed liquid B is added in the solution C, continue to stir, until the dichloromethane volatilization fully, microcapsule forms;
(5) after microcapsule forms, it is carried out decompress filter; Resulting microcapsule distilled water flushing, aeration-drying obtains Powdered microcapsule, kept dry.
The amount of solution C is added to and can makes dichloromethane volatilization fully, and forms microcapsule and get final product, does not have the prescription of strictness.
The present invention obtains microcapsule, prepares system with oil phase and compares, and surface of microcapsule is more smooth, and roundness is better, big or small homogeneous, and no adhesion phenomenon, particle size range are distributed in 200～300 μ m.In addition, because superoxide dismutase belongs to polar protein substance, the polar water reaction system that this law adopts helps keeping the biological activity of superoxide dismutase.
Description of drawings
The superoxide dismutase microcapsule sem photograph of Fig. 1: embodiment 1
The superoxide dismutase microcapsule sem photograph of Fig. 2: embodiment 2
The superoxide dismutase microcapsule sem photograph of Fig. 3: embodiment 3
The specific embodiment
Embodiment 1: respectively ethyl cellulose and cattle colostrums are pulverized, sieved.120 order ethyl celluloses after sieving are joined in the dichloromethane, and the limit edged stirs, and it is dissolved fully, obtains mixed liquor A.80 order cattle colostrumss after pulverizing and the superoxide dismutase mass ratio by 1: 1 is mixed, obtain the two mixture.The mixture of cattle colostrums and superoxide dismutase is slowly joined mixed liquor A, and the mixture of cattle colostrums and SOD and ethyl cellulose mass ratio are 5: 1, and the limit edged stirs, and to dissolving fully, obtain mixed liquid B and leave standstill.Get dodecyl sodium sulfate, be dissolved in the 10mL distilled water, be stirred to the solution clarification, obtain solution C, putting into water-bath is to stir under the 300rpm at rotating speed, 32 ℃ of bath temperatures.Mixed liquid B is added in the solution C, and continuing with the rotating speed is that 300rpm stirs, until the dodecyl sodium sulfate volatilization fully.After microcapsule forms, it is carried out decompress filter.Resulting microcapsule distilled water flushing, 40 ℃ of following aeration-drying 1 hour obtains Powdered microcapsule.With the rotating speed is 300rpm, and capsule pericardium material ratio is respectively that the microcapsule granule that condition under forms at 5: 1 is full, and adhesion is few, and roundness is good, and mean diameter is about 205 μ m.
Embodiment 2: respectively ethyl cellulose and cattle colostrums are pulverized, sieved.120 order ethyl celluloses after sieving are joined in the dichloromethane, and the limit edged stirs, and it is dissolved fully, obtains mixed liquor A.100 order cattle colostrumss after pulverizing and the superoxide dismutase mass ratio by 1: 1 is mixed, obtain the two mixture.The mixture of cattle colostrums and superoxide dismutase is slowly joined mixed liquor A, and the mixture of cattle colostrums and superoxide dismutase and ethyl cellulose mass ratio are 3: 1, and the limit edged stirs, and to dissolving fully, obtain mixed liquid B, leave standstill.Get dodecyl sodium sulfate, be dissolved in the 10mL distilled water, be stirred to the solution clarification, obtain solution C.Putting into water-bath is to stir under the 300rpm at rotating speed, 32 ℃ of bath temperatures.Mixed liquid B is added in the solution C, and continuing with the rotating speed is that 300rpm stirs, until the dodecyl sodium sulfate volatilization fully.After microcapsule forms, it is carried out decompress filter.Resulting microcapsule distilled water flushing, 45 ℃ of following aeration-drying 1 hour obtains Powdered microcapsule.With the rotating speed is 300rpm, and capsule pericardium material ratio is respectively that the microcapsule degree of adhesion that condition under forms at 3: 1 is better, and roundness is better, and mean diameter is about 187 μ m.
Embodiment 3: respectively ethyl cellulose and cattle colostrums are pulverized, sieved.60 order ethyl celluloses after sieving are joined in the dichloromethane, and the limit edged stirs, and it is dissolved fully, obtains mixed liquor A.120 order cattle colostrumss after pulverizing and the superoxide dismutase mass ratio by 1: 4 is mixed, obtain the two mixture.The mixture of cattle colostrums and superoxide dismutase is slowly joined mixed liquor A, and the mixture of cattle colostrums and superoxide dismutase and ethyl cellulose mass ratio are 5: 1, and the limit edged stirs, and to dissolving fully, obtain mixed liquid B, leave standstill.Get dodecyl sodium sulfate, be dissolved in the 10mL distilled water, be stirred to the solution clarification, obtain solution C.Putting into water-bath is to stir under the 300rpm at rotating speed, 28 ℃ of bath temperatures.Mixed liquid B is added in the solution C, and continuing with the rotating speed is that 300rpm stirs, until the dodecyl sodium sulfate volatilization fully.After microcapsule forms, it is carried out decompress filter.Resulting microcapsule distilled water flushing, 35 ℃ of following aeration-drying 2 hours obtains Powdered microcapsule.With the rotating speed is 300rpm, and capsule pericardium material ratio is respectively the microcapsule that condition under forms at 5: 1, and adhesion is few, and roundness is good, the about 253 μ m of mean diameter.
Embodiment 4: respectively ethyl cellulose and cattle colostrums are pulverized, sieved.90 order ethyl celluloses after sieving are joined in the dichloromethane, and the limit edged stirs, and it is dissolved fully, obtains mixed liquor A.80 order cattle colostrumss after pulverizing and the superoxide dismutase mass ratio by 4: 1 is mixed, obtain the two mixture.The mixture of cattle colostrums and superoxide dismutase is slowly joined mixed liquor A, and the mixture of cattle colostrums and superoxide dismutase and ethyl cellulose mass ratio are 5: 1, and the limit edged stirs, and to dissolving fully, obtain mixed liquid B, leave standstill.Get dodecyl sodium sulfate, be dissolved in the 10mL distilled water, be stirred to the solution clarification, obtain solution C.Putting into water-bath is to stir under the 400rpm at rotating speed, 32 ℃ of bath temperatures.Mixed liquid B is added in the solution C, and continuing with the rotating speed is that 400rpm stirs, until the dodecyl sodium sulfate volatilization fully.After microcapsule forms, it is carried out decompress filter.Resulting microcapsule distilled water flushing, 45 ℃ of following aeration-drying 2 hours obtains Powdered microcapsule.With the rotating speed is 400rpm, and capsule pericardium material ratio is respectively that the microcapsule granule that condition under forms at 5: 1 is full, and adhesion is few, and roundness is good, and mean diameter is about 270 μ m.
Embodiment 5: 120 order ethyl celluloses after will pulverizing, sieving join in the dichloromethane, and the limit edged stirs, and it is dissolved fully, obtains mixed liquor A.Pure superoxide dismutase is joined mixed liquor A, and superoxide dismutase and ethyl cellulose mass ratio are 5: 1, and the limit edged stirs, and to dissolving fully, obtain mixed liquid B, leave standstill.Get dodecyl sodium sulfate, be dissolved in the 10mL distilled water, be stirred to the solution clarification, obtain solution C.Putting into water-bath is to stir under the 400rpm at rotating speed, 32 ℃ of bath temperatures.Mixed liquid B is added in the solution C, and continuing with the rotating speed is that 400rpm stirs, until the dodecyl sodium sulfate volatilization fully.After microcapsule forms, it is carried out decompress filter.Resulting microcapsule distilled water flushing, 40 ℃ of following aeration-drying 1 hour obtains Powdered microcapsule.With the rotating speed is 400rpm, and capsule pericardium material ratio is respectively that the microcapsule granule that condition under forms at 5: 1 is full, and adhesion is few, and roundness is good, and mean diameter is about 220 μ m.
Claims (1)
1. the preparation method of the microencapsulation of a superoxide dismutase is characterized in that, may further comprise the steps:
(1) ethyl cellulose and cattle colostrums are pulverized, sieved respectively; Measure dichloromethane; After sieving 60 joined in the dichloromethane to 120 purpose ethyl celluloses, and the limit edged stirs, and it is dissolved fully, obtains mixed liquor A;
(2) 80 to 120 purpose cattle colostrumss after will pulverizing and superoxide dismutase mix by 1: 4 to 4: 1 mass ratio, obtain the two mixture; With cattle colostrums and superoxide dismutase mixture, perhaps pure superoxide dismutase joins mixed liquor A, and the limit edged stirs, and to dissolving fully, obtains mixed liquid B, leaves standstill; Cattle colostrums and superoxide dismutase mixture, the mass ratio of perhaps pure superoxide dismutase and ethyl cellulose is 3: 1 to 5: 1;
(3) dodecyl sodium sulfate is dissolved in the distilled water, is stirred to the solution clarification, obtain solution C, the water-bath temperature control stirs;
(4) mixed liquid B is added in the solution C, continue to stir, until the dichloromethane volatilization fully, microcapsule forms;
(5) after microcapsule forms, it is carried out decompress filter; Resulting microcapsule distilled water flushing, aeration-drying obtains Powdered microcapsule, kept dry.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200710177979XA CN101181632B (en) | 2007-11-23 | 2007-11-23 | Method for preparing superoxide dismutase microcapsule |
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| CN200710177979XA CN101181632B (en) | 2007-11-23 | 2007-11-23 | Method for preparing superoxide dismutase microcapsule |
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| CN101181632A CN101181632A (en) | 2008-05-21 |
| CN101181632B true CN101181632B (en) | 2010-09-08 |
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| CN102579401A (en) * | 2012-03-27 | 2012-07-18 | 山东省中医药研究院 | Gromwell pigment micro-capsules and preparation method thereof |
| CN105288652A (en) * | 2015-10-30 | 2016-02-03 | 珠海市雅莎生物科技有限公司 | Superoxide dismutase microcapsule powder and preparation method thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1748793A (en) * | 2005-07-18 | 2006-03-22 | 北京联合大学生物化学工程学院 | Method for preparing cow foremilk immune globulin microcapsulation |
| CN1830418A (en) * | 2005-03-08 | 2006-09-13 | 邹静中 | Microencapsulation method of superoxide dismutase |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1830418A (en) * | 2005-03-08 | 2006-09-13 | 邹静中 | Microencapsulation method of superoxide dismutase |
| CN1748793A (en) * | 2005-07-18 | 2006-03-22 | 北京联合大学生物化学工程学院 | Method for preparing cow foremilk immune globulin microcapsulation |
Non-Patent Citations (1)
| Title |
|---|
| 刘栋等.牛血超氧化物歧化酶的制备及其明胶微囊.中国生化药物杂志19 1.1998,19(1),6-11. * |
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