CN101169371A - Fluorescent quick detection device for benzopyrene (a) in food - Google Patents
Fluorescent quick detection device for benzopyrene (a) in food Download PDFInfo
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- CN101169371A CN101169371A CNA2007100099002A CN200710009900A CN101169371A CN 101169371 A CN101169371 A CN 101169371A CN A2007100099002 A CNA2007100099002 A CN A2007100099002A CN 200710009900 A CN200710009900 A CN 200710009900A CN 101169371 A CN101169371 A CN 101169371A
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Abstract
The invention discloses a fluorescence rapid detecting device of benzopyrene (a) in food, and relates to a fluorescence detecting device. The invention provides a fluorescence rapid detecting device with simple detection method, low device cost and rapid measurement. The invention is provided with an optical part, a control and signal process part and a power part. The optical part is provided with a light source, an excitation monochromator, a sample room, a sample tank, a low-temperature device, an emission monochromator and a detector. The control and signal process part is provided with a controller, a signal amplifying and differential module, an A/D transfer card and a computer. The power part is provided with a power source for the light source and a power source for the detector.
Description
Technical field
The present invention relates to a kind of fluorescence detection device, especially relate to the fluorescent quick detection device of benzo in a kind of food (a) pyrene.
Background technology
Many bad aromatic hydrocarbons (PAHs) compounds that to be at least 2 phenyl ring link to each other with the form of condensed ring is the carcinogenic organic contaminant of a class that exists in the environment, extensively is present in human lives's the physical environment such as atmosphere, water body, soil, crop and food.Benzo (a) pyrene is because widely distributed, stable in properties, and carcinogenicity is strong, becomes the representative substances of palycyclic aromatic.The approach of benzo (a) pyrene harm humans mainly is the pollution to food, and particularly benzo (a) pyrene content is higher in the processed food (as smoking food, baked goods and fried food etc.), so be necessary strict monitoring.
Benzo (a) pyrene has caused the attention widely of countries in the world to the pollution of food, and many countries have stipulated the limit standard of corresponding dietary intake and food.China allows limit standard be≤5ng/g (GB2762-2005) to the benzo in the food (a) pyrene, the limiting the quantity of well below China's standard (as German meat requirement≤1ng/g) of American-European countries's regulation.Along with China joined WTO, importing and exporting the seashore city will more and more higher to trace in the food until the examination requirements of palycyclic aromatics such as ultratrace benzo (a) pyrene, but because the complicacy of existing detection method, many quality testing departments fail really to carry out this work.
Mainly there are following three problems in the analysis of palycyclic aromatic in the food: most of palycyclic aromatic exists with trace level in (1) food, and easily further loss influences the testing result accuracy in extraction and purge process; (2) some organic compounds meetings and palycyclic aromatic are extracted out together, separation and detection by quantitative below disturbing; (3) structural similarity of a lot of palycyclic aromatics, the separation detection of these compounds is difficulty more.In order to overcome these problems, analytical work person need design a kind of device, benzo (a) pyrene of measuring in the food on this device can allow to reduce to greatest extent pre-treatment step, avoids the loss of object as far as possible, and benzo (a) pyrene is had higher selectivity.
At present, more to the instrument that the palycyclic aromatic (derivant) that contains in the food is analyzed, as gas chromatography-mass spectrography technology (GC/MS) (Md Yeakub Ali, Richard B Cole.J Agric Food Chem.2001,49 (9): 4192.), high performance liquid chromatography separates with fluoroscopic examination coupling (HPLC-FL) (1, Stijn F V, Kerkhoff M A T, Vandeginste B G M.JChromatogr A.1996,750 (1-2): 263; 2, Chen B H, Wang C Y, Chiu C P, J Agric Food Chem.1996,44:2244; 3, Kishikawa N, Wada M, Kuroda N, Akiyama S, Nakashima K.J.Chromatogr B, 2003,789:257.) etc.These instruments have stronger compartment analysis ability, but food samples for complexity, because between component or the interference of matrix, often need in addition pre-separation, and chromatographic resolution Mass Spectrometer Method coupling technique not only instrument and equipment and analysis cost costliness, operation simultaneously is also complicated and time-consuming, also must calibrate retention time with standard sample, and because the diluting effect of carrier has also reduced sensitivity relatively.
China's national standard is to the assay method (mensuration of benzo in the food (a) pyrene of benzo in the food (a) pyrene; the State Standard of the People's Republic of China; GB/T5009.27-2003.) there are two kinds; first kind is fluorescence spectrophotometry: sample is used earlier organic solvent extraction; or after saponification, extract; again extract is distributed or the chromatographic column purification through liquid-liquid; on acetylation filter paper, separate benzo (a) pyrene then; because of benzo (a) pyrene is the purple fluorescence spot under UV-irradiation; there will be the filter paper of benzo (a) pyrene partly to cut after will separating; after the solvent leaching, more quantitative with fluorospectrophotometer survey fluorescence intensity and standard.Though this detection method is the quantivative approach of authorized by state, the experimentation complexity is time-consuming, and sample loss is also more, and specified rate work brings a lot of inconvenience.Second kind is visual colorimetry: the sample pre-treatments step is identical with first kind of fluorescence spectrophotometry, its difference is isolated benzo (a) pyrene spot to be placed under the uviol lamp of wavelength 365nm in the mensuration stage and observes, and to carry out the visual colorimetry summary quantitative with standard blob.
In sum, benzo (a) pyrene that present various instrument (method) is measured in the food all will be at benzo (a) pyrene in extracting sample (for the high fatty foods of part, through extracting after the saponification) after, further purify and pre-separation through chromatographic column, just can deliver on the instrument and measure.Sample pre-treatments is quite loaded down with trivial details time-consuming, and the artificial amount that needs is big, the requirement that does not reach fast detecting, and, also may cause the loss of determinand benzo (a) pyrene because treatment step is many, reduce the confidence level of measuring.
The applicant provides the fluorescent rapid screening method of benzo in a kind of food (a) pyrene in the application for a patent for invention of publication number for CN1632535A, be immersed in the methylene chloride after food is shredded grinding, gets supernatant liquor and moves into sample cell; Use has the fluorospectrophotometer of derivative-permanent energy synchronous scanning, and its wavelength scanning range need comprise 350～420nm SPECTRAL REGION; Carry out constant-energy synchronous fluorescence spectrum or derivative-constant-energy synchronous fluorescence spectrum mapping; Data read; Data processing utilizes standard addition method to calculate.
Summary of the invention
The objective of the invention is to many shortcomings of existing at benzo (a) pyrene detecting instrument and method in the existing food, provide that a kind of method is simple, cost of equipment is low and measure the fluorescent quick detection device of benzo (a) pyrene in the food fast.
The present invention is provided with opticator, control and signal processing and power unit.
Opticator is provided with light source, excitation monochrometer, sample chamber, sample cell, Cryo Equipment, emission monochromator and detecting device, the mixed light that light source sends is after the excitation monochrometer beam split, the monochromatic excitation light that obtains enters the sample chamber and excites sample in the sample cell of being located in the sample chamber, the fluorescence that sample in the sample cell sends enters detecting device behind the emission monochromator splitting, Cryo Equipment is connected with the sample chamber through optical fiber.
Control is arranged with controller with signal processing part, signal amplifies and differential module, A/D transition card and computing machine, the input end of controller is connected with computing machine, the output terminal of controller is connected with the emission monochromator with excitation monochrometer respectively, signal amplifies the output termination A/D transition card with the differential module, and signal amplifies the input termination detector with the differential module.
Power unit is provided with light source power and detecting device power supply.The detecting device power supply is connected with detecting device, and light source power is connected with light source.
Controller can be provided with two groups of indicating circuits, wavelength detection and holding circuit and pulse distribution and driver module; indicating circuit detects with wavelength and is connected with holding circuit; wavelength detects the input termination computing machine with holding circuit; wavelength detects output termination pulse distribution and the driver module input end with holding circuit, and pulse distribution and driver module output terminal connect the stepper motor in excitation monochrometer or the emission monochromator respectively.The input end of controller can be connected with the LPT parallel port of computing machine.
Signal amplifies with the differential module can be provided with dark current compensation circuit, low noise amplifier, low-pass filter and second-order differential device, the dark current compensation circuit output terminal is connected with the detecting device of opticator respectively with the low noise amplifier input end, low noise amplifier output termination second-order differential device input end, the A/D transition card of second-order differential device output termination control and signal processing.
Described Cryo Equipment is made up of Dewar flask and low temperature sample cell, with the direct freezing sample of liquid nitrogen, safely and fast, and is directly connected to the sample chamber by optical fiber, replaces conventional sample cell, need not just reequip instrument and can realize low temperature functional easily.
Control Software of the present invention can adopt the C language compilation, may command controller receiving computer sends pulse command and passes to pulse distribution and driver module distributes, be sent to excitation monochrometer and the stepper motor of emission in the monochromator more respectively, drive that it is poor with constant energy
Relation rotate simultaneously, realize derivative one constant-energy synchronous fluorescence spectral scan.
Control Software of the present invention can go out Feng Chu at predefined benzo (a) pyrene automatically and read signal, and sample mark-on concentration carried out linear fit and the online anti-calculating that pushes away, directly provide the original content of benzo in the sample (a) pyrene, and with country to the limit standard of benzo in the food (a) pyrene relatively, whether judgement sample qualified.
Compare with method with benzo (a) pyrene detecting instrument in the existing food, outstanding advantage of the present invention is:
1. use the present invention to detect benzo (a) pyrene, for bread and cheese only need simply soak the extraction or ultrasonic extraction (for example 40min) (for high fatty foods, after saponification, extract), need not to carry out again loaded down with trivial details time-consuming purifying and pre-separation step, the test spectral that promptly is available on the machine is obtained the content of benzo in the food (a) pyrene, has simplified sample pretreatment process (referring to embodiment and Fig. 6) greatly.2. can scan the derivative one constant-energy synchronous fluorescence spectrum of benzo in the food (a) pyrene feature, the detection of benzo (a) pyrene is had good selectivity.3. can scan low temperature one derivative one impossible energy synchronous fluorescent spectrum, suppress of the spectrum interference of other palycyclic aromatic effectively benzo (a) pyrene.4. can eliminate the interference of matrix complicated in the food, make the quantitatively more accurate of benzo (a) pyrene benzo (a) pyrene.5. can carry out linear fit automatically and instead push away calculating, provide the original content of benzo in the unknown sample (a) pyrene immediately, and with country to the limit standard of benzo in the food (a) pyrene relatively, whether judgement sample qualified; Can preserve simultaneously the pick of original spectrum number, further analyze for do in the future.6. of the present invention easy to operate, highly sensitive, can reach 0.07ng/g to the detection limit of benzo (a) pyrene, well below China the benzo in the food (a) pyrene is allowed limit standard, even be lower than the limit standard of European countries.
Description of drawings
Fig. 1 is that the structure of the embodiment of the invention is formed synoptic diagram.
Fig. 2 is the structure composition frame chart of the controller part of the embodiment of the invention.
Fig. 3 is the signal amplification of the embodiment of the invention and the composition frame chart of differential module.
Fig. 4 is the signal amplification of the embodiment of the invention and the circuit theory diagrams of differential module.
Fig. 5 is spectrometric concrete operations process flow diagram of the embodiment of the invention.In Fig. 5, box indicating is carried out by operating personnel, and ellipse representation is carried out by computing machine, and rhombus is represented the judgement that need elect.
Fig. 6 compares synoptic diagram for the mensuration flow process of benzo (a) pyrene detecting instrument and method in use the present invention and existing other food.In Fig. 6, empty frame table shows the operation that need take for high fatty foods, need not this operation for bread and cheese.
Embodiment
Following examples will the present invention is further illustrated in conjunction with the accompanying drawings.
Referring to Fig. 1～3, the present invention is provided with opticator I, control and signal processing II and power unit III.Opticator I is provided with light source 1, excitation monochrometer 2, sample chamber 3, sample cell 4, Cryo Equipment 7, emission monochromator 5 and detecting device 6, the mixed light that light source 1 sends is after excitation monochrometer 2 beam split, the monochromatic excitation light 17 that obtains enters sample chamber 3 and excites sample in the sample cell of being located in the sample chamber 34, the fluorescence 18 that sample in the sample cell 4 sends enters detecting device 6 after 5 beam split of emission monochromator, Cryo Equipment 7 is connected with sample chamber 3 through optical fiber 8.Cryo Equipment 7 is made up of Dewar flask and low temperature sample cell, with the direct freezing sample of liquid nitrogen, safely and fast, and is directly connected to sample chamber 3 by optical fiber 8, replaces conventional sample cell 4, need not just reequip instrument and can realize low temperature functional easily.Control is provided with controller 12 with signal processing II, signal amplifies and differential module 13, A/D transition card 11 and computing machine 10, the input end of controller 12 is connected with the LPT parallel port of computing machine, controller output end is connected with emission monochromator 5 with excitation monochrometer 2 respectively, signal amplifies the output termination A/D transition card 11 with differential module 13, and signal amplifies the input termination detector 6 with differential module 13.
Signal amplifies and differential module 13 is provided with dark current compensation circuit 23, low noise amplifier 24, low-pass filter 25 and second-order differential device 26, dark current compensation circuit 23 output terminals are connected with the detecting device 6 of opticator respectively with low noise amplifier 24 input ends, low noise amplifier 24 output termination second-order differential devices 26 input ends, the A/D transition card of 26 output termination controls of second-order differential device and signal processing.
Power unit III is provided with light source power 15 and detecting device power supply 14.Detecting device power supply 14 connects detecting device 6, and light source power 15 connects light source 1.
Control Software of the present invention can adopt the C language compilation, can go out Feng Chu at predefined benzo (a) pyrene automatically and read signal, and sample mark-on concentration is carried out linear fit and the online anti-calculating that pushes away, and directly provides the original concentration of benzo in the sample (a) pyrene.
Since adopted constant-energy synchronous fluorescence scan mode (1, Inman E L, Winefordner Jr J D, Anal.Chem., 1982,54:2018; 2, He L F, Lin D L, Li Y Q, Anal.Sci., 2005,21:641; 3, Li Y Q, HuangX Z, Xu J G, Chen G Z, Anal.Chim.Acta, 1992,256:285.), exciting light 17 is in scanning process and the light of revocable single wavelength, but continually varying, and with fluorescence that detecting device 6 receives specific relation is arranged
This makes by selecting suitable parameters
Allow the present invention that the detection of benzo (a) pyrene has been had good selectivity.And derivative technique (1, Li Y.Q.et al..Fresenius J.Anal.Chem., 1997,357:1072.; 2, Lin D.L.et al..Luminescence, 2005, application 20:292.) allows this device further reduce spectrum and disturbs, and gets rid of matrix and disturbs, thereby help improving sensitivity for analysis.
For palycyclic aromatic component complicated sample, available Cryo Equipment 7 replaces conventional sample cell 4, scanning low temperature-derivative-constant-energy synchronous fluorescence spectrum, with the interference that suppresses other palycyclic aromatics (1, Kirkbright GF and De Lima CG..Analyst.1974,99:338; 2, Zhang W et al..Talanta, 2007,71:1481.), make that the assay of benzo (a) pyrene is more accurate.Cryo Equipment 7 is made up of Dewar flask and low temperature sample cell, and Dewar flask can adopt the commercially available prod, and the low temperature sample cell adopts the applicant's the described cryogenic tank sample cell of " the cryogenic tank sample cell that is used for fluorospectrophotometer " (patent No. is CN2498605Y).In Dewar flask, pour into liquid nitrogen during use, again the low temperature sample cell is immersed freezing sample acquisition cryogenic effect in the liquid nitrogen.Cryo Equipment 7 is connected to sample chamber 3 by an optical fiber 8, and an end of optical fiber 8 is inserted in the Dewar flask, aims at the low temperature sample cell, and the other end is connected with optical coupler 9.During use as long as optical coupler 9 is placed on the position of normal temperature conventional sample cell 4 of when experiment in sample chamber 3.
Fig. 4 provides the signal amplification of the embodiment of the invention and the circuit theory diagrams of differential module.The electric signal of sending here by detecting device, being sent to signal amplifies and the differential module, carrying out signal through low noise micro current amplifier (AD549JH) earlier amplifies, be sent to operational amplifier (LF351) elimination noise again, then be sent to the second-order differential device that constitutes by 2 operational amplifiers among a slice LF353 the time is carried out differential, be sent to the A/D transition card then and carry out the A/D conversion.
Below provide the reference model and the reference value of the main components and parts among Fig. 4:
Operational amplifier U1:AD549JH type, U2, U3A, U3B:LF353 type;
Voltage stabilizing diode D1, D2:7V;
Capacitor C 41:330PF, C42, C43, C46, C48, C50, C51, C1:0.1 μ F, C44, C47, C49:1 μ F, C45:0.47 μ F;
Potentiometer VR14:25K, VR3, VR22:33K;
Combination hub J1 (COM6), J2 (COM2), J3 (COM4).
Spectrometric concrete operations process flow diagram of the present invention sees also Fig. 5, and wherein box indicating is carried out by operating personnel, and ellipse representation is carried out by computing machine, and rhombus is represented the judgement that need elect.Introduce its operating process below in conjunction with Fig. 5:
(1) opens the shift knob of computing machine and miscellaneous part, move Control Software of the present invention.
(2) computing machine is assigned instruction to controller, and wavelength calibration is carried out in the self check of control monochromator.
(3) parameter is set, comprises that monochromator slit width, photomultiplier negative high voltage, sensitive shelves, constant energy are poor, sweep velocity and scanning wavelength scope.Under the overwhelming majority situation, these parameters all are changeless, only need to adopt default setting, do not need each the modification to set.
(4) accurately pipette the sample that certain volume is handled well with transfer pipet, put into sample cell.
(5) the mark-on concentration number that will preserve automatically of computing machine is initialized as 0 (N=0).
(6) in sample, add high concentration benzo (a) the pyrene standard model storing solution of small volume accurately with micro syringe.
(7) scanning derivative-constant-energy synchronous fluorescence spectrum.
(8) preserve spectroscopic data.
(9) judge whether to preserve mark-on concentration.Select under the normal condition to be, preserve mark-on concentration, enter next link (10); If operating process is made a fault or other reasons and to cause spectrum to take place unusual, then select not, do not preserve mark-on concentration, skip next link (10), enter link (11).
(10) the mark-on concentration number that will preserve automatically of computing machine increases by 1 (N=N+1).
(11) computing machine judges automatically whether mark-on concentration number N meets or exceeds 4.If N 〉=4 enter next link (12), otherwise skip next link (12), enter link (13).
(12) computing machine goes out the peak position at benzo (a) pyrene automatically and reads signal, and sample mark-on concentration is carried out linear fit and the online anti-calculating that pushes away, and directly provides the original content of benzo in the sample (a) pyrene, is shown on the screen.
(13) judge whether to carry out mark-on next time.If data point is sufficient inadequately, selection is to re-execute link (6)～(12); Otherwise select not enter link (14).
(14) the original content with benzo in the sample (a) pyrene compares the limit standard of benzo in the food (a) pyrene with country, and whether judgement sample is qualified, and the result is presented on the screen.
(15) finish spectroscopic assay, close instrument.
Fig. 6 provides the mensuration flow process comparison synoptic diagram that uses benzo (a) pyrene detecting instrument and method in the present invention and existing other food.As seen from Figure 6, (for example the gas phase look becomes spectrometer with using benzo (a) pyrene detecting instrument in the existing food except the present invention in sample extraction stage (comprising sample extraction and saponification step) and test phase, mass spectrometer, high performance liquid chromatograph, the range estimation of fluorospectrophotometer or naked eyes) beyond basic identical, the present invention need not to carry out loaded down with trivial details time-consuming purifying again and the pre-separation step (uses benzo in the existing food (a) pyrene detecting instrument at purifying, preliminary separation stage needs chromatographic column to purify, steps such as chromatography), the test spectral that promptly is available on the machine is obtained the content of benzo in the food (a) pyrene, has simplified sample pretreatment process greatly.
Claims (5)
1. the fluorescent quick detection device of benzo (a) pyrene in the food is characterized in that being provided with opticator, control and signal processing and power unit;
Opticator is provided with light source, excitation monochrometer, sample chamber, sample cell, Cryo Equipment, emission monochromator and detecting device, the mixed light that light source sends is after the excitation monochrometer beam split, the monochromatic excitation light that obtains enters the sample chamber and excites sample in the sample cell of being located in the sample chamber, the fluorescence that sample in the sample cell sends enters detecting device behind the emission monochromator splitting, Cryo Equipment is connected with the sample chamber through optical fiber;
Control is arranged with controller with signal processing part, signal amplifies and differential module, A/D transition card and computing machine, the input end of controller is connected with computing machine, the output terminal of controller is connected with the emission monochromator with excitation monochrometer respectively, signal amplifies the output termination A/D transition card with the differential module, and signal amplifies the input termination detector with the differential module;
Power unit is provided with light source power and detecting device power supply, and the detecting device power supply connects detecting device, and light source power connects light source.
2. the fluorescent quick detection device of benzo (a) pyrene in a kind of food as claimed in claim 1; it is characterized in that controller is provided with two groups of indicating circuits, wavelength detection and holding circuit and pulse distribution and driver module; indicating circuit detects with wavelength and is connected with holding circuit; wavelength detects the input termination computing machine with holding circuit; wavelength detects output termination pulse distribution and the driver module input end with holding circuit, and pulse distribution and driver module output terminal connect the stepper motor in excitation monochrometer or the emission monochromator respectively.
3. the fluorescent quick detection device of benzo (a) pyrene in as claimed in claim 1～kind of food, it is characterized in that signal amplifies and the differential module is provided with dark current compensation circuit, low noise amplifier, low-pass filter and second-order differential device, the dark current compensation circuit output terminal is connected with the detecting device of opticator respectively with the low noise amplifier input end, low noise amplifier output termination second-order differential device input end, the A/D transition card of second-order differential device output termination control and signal processing.
4. the fluorescent quick detection device of benzo (a) pyrene in a kind of food as claimed in claim 1 is characterized in that described Cryo Equipment is provided with Dewar flask and low temperature sample cell.
5. the fluorescent quick detection device of benzo (a) pyrene in a kind of food as claimed in claim 1, it is characterized in that Control Software control controller receiving computer sends pulse command and passes to pulse distribution and driver module distributes, be sent to excitation monochrometer and the stepper motor of emission in the monochromator more respectively, drive that it is poor with constant energy
Relation rotate simultaneously.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101957374A (en) * | 2010-09-17 | 2011-01-26 | 无锡安迪生物工程有限公司 | Benzopyrene rapid detection card |
| CN102426162A (en) * | 2010-12-31 | 2012-04-25 | 广州万孚生物技术有限公司 | Fluorescence quantitative detector |
| CN102809624A (en) * | 2011-05-30 | 2012-12-05 | 株式会社岛津制作所 | Method and system for synchronously driving light-dispersing elements, and detector for chromatograph |
| CN102818791A (en) * | 2012-02-09 | 2012-12-12 | 龙岩学院 | Fluorescence detection method of polycyclic aromatic hydrocarbons (PAHs) in water body |
| CN102914523A (en) * | 2011-08-04 | 2013-02-06 | 刘先利 | Synchronous fluorimetry for simply measuring pyrene in fish gills |
| CN104914143A (en) * | 2015-05-11 | 2015-09-16 | 同济大学 | Photoelectric analysis method of benzopyrene through in-situ molecularly imprinted functionalized CdS/3DOM TiO2/BDD electrode |
| CN105092318A (en) * | 2015-08-14 | 2015-11-25 | 中国石油化工股份有限公司 | Method for sampling benzo a pyrene in asphalt tank headspace gas |
| CN105954258A (en) * | 2016-05-26 | 2016-09-21 | 伯格森(北京)科技有限公司 | Detector and detection method for edible oil doped with inferior oil |
| CN112557158A (en) * | 2021-02-28 | 2021-03-26 | 中国工程物理研究院核物理与化学研究所 | Separation, purification and collection device for xenon in air sample |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101957374A (en) * | 2010-09-17 | 2011-01-26 | 无锡安迪生物工程有限公司 | Benzopyrene rapid detection card |
| CN102426162A (en) * | 2010-12-31 | 2012-04-25 | 广州万孚生物技术有限公司 | Fluorescence quantitative detector |
| CN102809624A (en) * | 2011-05-30 | 2012-12-05 | 株式会社岛津制作所 | Method and system for synchronously driving light-dispersing elements, and detector for chromatograph |
| CN102809624B (en) * | 2011-05-30 | 2014-06-25 | 株式会社岛津制作所 | Method and system for synchronously driving light-dispersing elements, and detector for chromatograph |
| CN102914523A (en) * | 2011-08-04 | 2013-02-06 | 刘先利 | Synchronous fluorimetry for simply measuring pyrene in fish gills |
| CN102818791A (en) * | 2012-02-09 | 2012-12-12 | 龙岩学院 | Fluorescence detection method of polycyclic aromatic hydrocarbons (PAHs) in water body |
| CN104914143A (en) * | 2015-05-11 | 2015-09-16 | 同济大学 | Photoelectric analysis method of benzopyrene through in-situ molecularly imprinted functionalized CdS/3DOM TiO2/BDD electrode |
| CN104914143B (en) * | 2015-05-11 | 2017-08-25 | 同济大学 | A kind of molecular imprinting functionalization CdS/3DOM TiO in situ2The photoelectric analysis method of/BDD electrode pair BaPs |
| CN105092318A (en) * | 2015-08-14 | 2015-11-25 | 中国石油化工股份有限公司 | Method for sampling benzo a pyrene in asphalt tank headspace gas |
| CN105954258A (en) * | 2016-05-26 | 2016-09-21 | 伯格森(北京)科技有限公司 | Detector and detection method for edible oil doped with inferior oil |
| CN112557158A (en) * | 2021-02-28 | 2021-03-26 | 中国工程物理研究院核物理与化学研究所 | Separation, purification and collection device for xenon in air sample |
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