CN101168051A - Application of sea collagen peptide as preparation of anti-oxidation medicine, health-care food, food or cosmetics - Google Patents
Application of sea collagen peptide as preparation of anti-oxidation medicine, health-care food, food or cosmetics Download PDFInfo
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Abstract
The invention discloses the usage of an ocean collagen peptide preparing for making into antioxidant drugs, antioxidant health food, antioxidant food or antioxidant cosmetics. The products of the ocean collagen peptide have the characteristics of easy absorption, rapid absorption, low viscosity, perfect water-solubility and the like. Animal experiments show that the vitality of antioxidant enzymes is markedly strengthened by providing the ocean collagen peptide for a bandicoot, the content of overoxidation lipoid is reduced, and the invention has the effect of oxidation resistance. Clinical experiments also prove that through oral administration of the ocean collagen peptide, the activity of the antioxidant enzymes of human body can be reduced, generation of lipoid peroxide can be reduced, thereby having the function of oxidation resistance, and facial chloasmata and the like can be dispelled.
Description
Technical field:
The present invention relates to a kind of new purposes of marine bioactivity oligopeptide, is that the marine collagen that extracts from marine fishes is as the purposes in preparation anti-oxidation medicine, anti-oxidation health food, antioxidant food or the cosmetics specifically.Belong to medicine, health care of food and cosmetic field.
Background technology:
The development of modern society, the health that adds ambassador modern of the deterioration of living environment and social pressure is subjected to serious threat, free radical causes the body injury degeneration, is the basic reason that causes a series of diseases such as body aging, tumor, cardiovascular disease, oculopathy, diabetes.Caused that people more pay attention to, the consumption figure of antioxidant is increasing.But the toxicity, side effect of the chemical antioxidants of volume of production maximum just progressively makes it be restricted, so people progressively turn to sight the research of the Natural antioxidant.
Biologically active peptide is meant the peptide matters with special physiological function.The Bayliss of London University's medical college in 1902 and Startling have found a kind ofly can cause the active material of pancreatic secretion from the gastrointestinal of animal, are called secretin, and this is the human biological activity peptide matters of finding for the first time.After this, be accompanied by the develop rapidly of biochemistry and Protocols in Molecular Biology, the research of peptide has obtained breakthrough, has become the new focus of present research.
Peptide and protein all are aminoacid with the interconnection chemical compound that forms of amido link together, and the aminoacid degree of polymerization of different is peptide is little, and polymerization degree of protein is big.Structurally, peptide is in aminoacid and proteinic centre position, but it has aminoacid and the irreplaceable function of protein.For a long time, people only are used as a kind of nutritious composition to food protein, think just can be absorbed after protein only is hydrolyzed into free amino acid.But discovering in recent years, protein are after the hydrolysis of digestive tract enzymatic, and mainly the oligopeptide form with 10 or 10 following aminoacid compositions is absorbed.Oligopeptide is more effective than the absorption of single amino acids, and can participate in proteinic synthesizing directly, has therefore improved body to proteinic utilization rate.Occur under the situation of obstacle in aminoacid transportation system function, take in oligopeptide and but can obtain good assimilation effect.Having found hundreds of kind peptide in the organism, is that body is finished the active requisite participant of various complex physical.
Occurring in nature exists a large amount of biologically active peptides, and they play important effect in biological activity, has antioxidation, antibiotic, antitumor, protection cardiovascular, immunomodulating, the anti-ageing effect of waiting for a long time respectively.Use these bioactive peptide and can be used as medicine, vaccine and health product etc.Studies have shown that in a large number, when lacking some peptides of regulating physiological function in the human body, can cause the change of function of human body.Therefore from threpsology's angle, with when satisfying the essential amino acid needs, suitably replenish some biologically active peptide and all have far reaching significance strengthening diseases prevention, resistance against diseases and slow down aging in the eating patterns adjustment.
The ocean is the abundantest field of resource on the earth, since the ecological environment of marine organisms species than the terrestrial organism complexity many, it gives halobiontic some special chemical constitution is still undiscovered in the terrestrial life body, and this makes the ocean become the resource treasure-house of original new drug and functional health-care food.Since the seventies in 20th century, people have isolated the novel and special high activity of hundreds thousand of kinds of chemical constitutions, the bioactive substance of high-drug-effect from marine organisms, comprise peptide class, protein-based, polysaccharide, alkaloids, terpenoid, Macrocyclic polyester class etc.The peptide class is the hugest compounds of quantity in the marine bioactivity material, reach tens thousand of kinds more than, (company standard number is Q/HSBS1-2006 to the marine collagen that Zhejiang Hai Shi bio tech ltd is produced, the number of putting on record of Zhoushan Zhejiang Bureau of Technical Supervision is Q330900.X20.1121-2006, and hygiene permits is No. the 33090310003rd, Zhe Wei food card word 2006) be exactly one of them.Marine collagen is that the fish skin of marine fishes (Oncorhynchi, anglerfish, salmon fish, salmon, tilapia etc.) is a raw material to originate widely, the molecular weight that produces by hydrolysis is at 130～3000 oligo peptide, have easy absorption, absorb fast, low viscosity, characteristics such as good water solubility, proteinic as a supplement nutrient additive for food only at present.Up to now, any report of also in antioxidant drug, anti-oxidation health food, antioxidant food and antioxidation cosmetic product, not using about marine collagen.
Though, Chinese patent application number 200510000032.2 disclosed a kind of " oxidation resistant bioactive peptide of halobios and preparation method thereof ", be the marine organisms antioxidant activity polypeptide that extracts from the seashells visceral mass, still, it is all different with marine collagen with structure to originate.Chinese patent application number 03129075.2 discloses " a kind of biological preparation that contains marine active substance and uses thereof ", said preparation primary raw material composition is phycobniliprotein and polysaccharides, also comprise Radix Ginseng extract, vitamin E and pharmaceutic adjuvant, also different with marine collagen.
Summary of the invention:
The present invention is in order to solve the big problem of existing in prior technology chemical antioxidants toxicity, side effect, a kind of easy absorption be provided, absorb fast, low viscosity, good water solubility and the low purposes of low-molecular-weight marine collagen in preparation anti-oxidation medicine, preparation anti-oxidation health food, food or cosmetics of cost, provide a kind of new way for improving antioxidant ability of organism by diet.
Technical solution of the present invention is: the purposes of a kind of marine collagen in preparation anti-oxidation medicine, preparation anti-oxidation health food, food or preparation antioxidation cosmetic product.
Described medicine or health food are powder, granule, capsule, tablet, pill, oral liquid.
Described food is starch-based product, breast or milk product, bean product, baking goods.
Described cosmetics are emulsion, cream frost, oil preparation, water preparation, gel, aerosol or facial film.
The present invention has found that existing material-marine collagen has the antioxidative function, invented the purposes of marine collagen, characteristics such as its product has easy absorption, absorbs soon, low viscosity, good water solubility as preparation anti-oxidation medicine, anti-oxidation health food, antioxidant food or antioxidation cosmetic product.Zoopery is found, gives the vigor that the rat marine collagen can significantly strengthen antioxidase, reduces the content of lipid peroxidation pledge, has the antioxidative effect.Clinical experiment confirms that also oral marine collagen can improve the generation of human body activities of antioxidant enzymes and reduction lipid peroxide and have anti-oxidation function, can dispel chloasma faciei etc.
The specific embodiment:
The present invention is further illustrated below in conjunction with the specific embodiment.
Embodiment:
(company standard number is Q/HSBS1-2006 to the marine collagen of producing with Zhejiang Hai Shi bio tech ltd, the number of putting on record of Zhoushan Zhejiang Bureau of Technical Supervision is Q330900.X20.1121-2006) be raw material, can in preparation anti-oxidation medicine, anti-oxidation health food, antioxidant food or antioxidation cosmetic product, use.Marine collagen and other are medicinal or edible adjuvant is made anti-oxidation medicine and anti-oxidation health food can be powder, granule, capsule, tablet, pill, oral liquid etc.; Common antioxidant food is that marine collagen is added on starch-based product, milk and milk products, bean product, baking goods is medium.The antioxidation cosmetic product are that marine collagen is added in the existing cosmetics, make emulsion, cream frost, oil preparation, water preparation, gel, aerosol or facial film etc.
The experimentation of the embodiment of the invention is as follows:
1. to the antioxidation of experimental rat
72 of cleaning level male SD rats, 12 monthly ages, provide by Department Of Medicine, Peking University's Experimental Animal Center, approval number is SCXK11-00-0004, cleaning level Animal House is raised 25 ℃ of feeding environment temperature, humidity 60%RH.Be divided into 6 groups at random by body weight, be respectively 1～3 group of basic, normal, high dosage group of marine collagen, 4 groups of positive control vitamin E groups, 5 group model matched groups, 6 groups of blank groups.The D-galactose of 1～5 group of rat lumbar injection every day 125mg/kg continuous 3 months, causes the peroxide injury model; 6 groups of lumbar injections are with the volume normal saline.
Experimental session, 1～3 group of rat per os every day gives the marine collagen of variable concentrations, and dosage is respectively 0.225g/kg, 0.450g/kg and 1.35g/kg, is equivalent to 5,10 and 30 times of people's consumption every day respectively; 4 groups give positive control vitamin E 200mg/kg; 5 groups and 6 groups give with the volume distilled water.Irritate the stomach amount and press the 0.1ml/10g weighing machine, continuous irrigation stomach 3 months.Put to death animal when experiment finishes, carry out in the blood detection of superoxide dismutase (SOD) vigor in lipid peroxide catabolite malonaldehyde (MDA) content, the blood and catalase (CAT) vigor according to Ministry of Public Health " the health food function assessment assessment process and the method for inspection ".
Compare with model control group, irritate stomach with preparation of the present invention and make experimental group rat anti oxidase SOD and CAT vigor significantly strengthen (p＜0.05), lipid peroxide catabolite MDA content significantly reduces (p＜0.05), sees Table 1.
The result shows that product of the present invention can play anti-oxidation efficacy by raising activities of antioxidant enzymes, reduction lipid peroxidation.
Table 1 preparation of the present invention is to the influence of rat anti oxidative function
| Group | n | SOD | CAT | MDA |
| Dosage group marine collagen high dose group vitamin E positive controls model control group blank group in the marine collagen low dose group marine collagen | 12 12 12 12 12 12 | 455.52±11.39 * 460.15±18.09 * 468.59±27.25 * 474.99±24.87 * 395.56±15.25 476.55±15.22 * | 21.33±4.82 * 20.47±3.01 21.69±1.68 * 17.92±3.60 17.14±2.81 21.47±4.47 * | 5.85±0.78 5.67±0.93 * 5.76±1.02 * 5.52±1.14 * 7.63±1.37 5.14±1.11 * |
*Expression has significance, P＜0.05 with the model control group comparing difference
2. marine collagen anti-oxidation function clinical observation experiment
2.1 object and method
(1) tried thing: the marine collagen peptide capsule is provided by Zhejiang Hai Shi bio tech ltd.
(2) experimenter includes standard in: press the principle of voluntariness and select 45-65 year, physical condition is good, does not have obvious brain, the heart, liver, lung, kidney, blood illness, does not have the history of taking medicine for a long time, and aspiration is tried to guarantee the crowd that cooperates.
(3) eliminator's standard:
1. gestation or women breast-feeding their children are to this health food allergy sufferers.
2. merge to have the inclination, serious disease patients such as liver, kidney and hemopoietic system.
3. take the article relevant in a short time, have influence on judgement person the result with being tried function.
4. do not meet the standard of including in, eat in accordance with regulations and tried thing, can't judge effect or data not umbra sound effect or safety judgement person.
(4) grouping and contrast form: 100 routine experimenters are divided into test-meal group and matched group at random by MDA, SOD, GSH-PX level, adopt two kinds of contrast forms between self and group.Keep on ordinary days life and dietary habit during being tried.
(5) taking dose and time: the test-meal group is taken marine collagen, and each 3, every day 3 times.Took 90 days.Matched group is a blank, and two groups of former lives during the test-meal, dietary habit are constant.
(6) instrument and reagent: MEK-6138K type blood counting instrument, MIDIRON urinates ten analysers, the RA1000 automatic clinical chemistry analyzer, AMS FT-2 type spectrophotometer, the biochemical reagents box is all provided by middle living company.
2.2 observation index: each is checked once before and after the experiment.
(1) effect index
1. observation of symptoms: detailed medical history-taking, observe main clinic symptoms: feeling of fatigue, agitation, forgetful, sleep, soreness of waist gonalgia etc.Before and after test-meal, add up integrated value by symptom weight (serious symptom 3 minutes, moderate 2 minutes, light disease 1 minute), and improve (2 fens produce effects of each doing well,improving are improved 1 and are divided into effectively), calculate the improvement rate with regard to its cardinal symptom.
2. the antioxidation index detects
I. mda content: changing value and the MDA decline percentage rate of observing MDA before and after the test-meal.
II. superoxide dismutase: changing value and the SOD rising percentage rate of observing SOD before and after the test-meal.
III. glutathione peroxidase: changing value and the GSH-PX rising percentage rate of observing GSH-PX before and after the test-meal.
(2) safety indexes
1. general situation: comprise spirit, sleep, diet, defecation, blood pressure etc.
2. blood and urine, just routine examination: red blood cell count(RBC), hemoglobin, numeration of leukocyte is urinated ten detections, just conventional sense.
3. biochemical indicator is measured: serum albumin ALB, total protein TP, darling renal function (glutamic oxaloacetic transaminase, GOT AST, glutamate pyruvate transaminase ALT, carbamide UREA, creatinine CRE), blood glucose GLU, blood fat (T-CHOL TC, triglyceride TG).
4. Abdominal B type ultrasonography, electrocardiogram, the fluoroscopy of chest of X line is all checked once before the test-meal.
5. untoward reaction is observed
2.3 date processing and result judge
(1) date processing: with the STATE of statistical software 6.0 computational analysis data.All own control data adopt paired t-test, two groups of means relatively adopt t check in groups, and the latter need carry out homogeneity test of variance, and the data of nonnormal distribution or heterogeneity of variance are carried out suitable variable conversion, after waiting to satisfy the normal state homogeneity of variance, carry out the t check with data converted; If translation data still can not satisfy the requirement of normal state homogeneity of variance, use t ' check or rank test instead; But the coefficient of variation is too big as data (CV＞50%) is used rank test.The X 2 test of effect index.
(2) result judges
Effectively: mda content, superoxide dismutase, each experimental result positive in three experiments of glutathione peroxidase, promptly self relatively and between the group of test back relatively has statistical significance before and after the test.This given the test agent of decidable has the anti-oxidation function effect.
Invalid: mda content, superoxide dismutase, none positive as a result in three experiments of glutathione peroxidase.
2.4 experimental result
(1) general situation
Observe experimenter's 100 examples altogether, the male of observation group 11 examples, women's 39 examples, minimum 45 years old of age, maximum 65 years old, average 56.84 ± 5.55 years old.Matched group male 8 examples, women's 42 examples, minimum 47 years old of age, maximum 65 years old, average 55.14 ± 5.00 years old.By table 2 as seen, every index no significant difference before two groups of test-meals has comparability.
Situation comparison as table 2 observation is last (X ± SD)
| Group | Example number (example) | MDA(nmol/ml) | SOD(U/ml) | GSH-PX(U/ml) |
| Observation group's matched group | 50 50 | 9.46±3.18 9.49±3.14 | 78.38±18.94 77.84±16.29 | 79.03±15.12 81.22±16.58 |
(2) mda content changes
By table 3 as seen, every index no significant difference before two groups of test-meals, test-meal group was on request taken marine collagen 90 days, and test-meal group lipid peroxide catabolite malonaldehyde reduces by 2.59 ± 3.67nmol/ml, decline percentage rate 27.38%, contrast all has significant difference between own control and group.The matched group malonaldehyde reduces by 0.34 ± 3.27nmol/ml, and decline percentage rate 3.58% changes no significance before and after the test-meal.
Table 3 mda content changes unit (nmol/ml)
| Group | Example number (example) | Before the test-meal | After the test-meal | Difference | Decline percentage rate % |
| Observation group's matched group | 50 50 | 9.46±3.18 9.49±3.14 | 6.87±3.57 9.16±3.85 | 2.59±3.678 ** ## 0.34±3.27 | 27.38 3.58 |
Compare before and after self
*##＜0.01 is compared in P＜0.01 between group
(3) superoxide dismutase changes:
By table 4 as seen, every index no significant difference before two groups of test-meals, test-meal group was on request taken marine collagen 90 days, test-meal group superoxide dismutase rising 2.62 ± 18.48U/ml, rising percentage rate 3.34% contrasts there was no significant difference between own control and group.Matched group superoxide dismutase rising 0.26 ± 8.77U/ml, rising percentage rate 0.53% changes no significance before and after the test-meal.
Table 4 superoxide dismutase changes unit (U/ml)
| Group | Example number (example) | Before the test-meal | After the test-meal | Difference | Rising percentage rate % |
| Observation group's matched group | 50 50 | 78.38± 18.94 77.84± 16.29 | 80.99± 19.86 77.58± 14.41 | -2.62±18.84 -0.26±8.77 | 3.34 0.53 |
(4) glutathione peroxidase changes:
By table 5 as seen, every index no significant difference before two groups of test-meals, test-meal group was on request taken marine collagen 90 days, test-meal group glutathione peroxidase rising 10.93 ± 19.74U/ml, rising percentage rate 13.83%, contrast has significant difference between own control and group.Matched group glutathione peroxidase rising 0.56 ± 13.74U/ml, malonaldehyde reduces by 0.34 ± 3.27nmol/ml, and rising percentage rate 0.69% changes no significance before and after the test-meal.
Table 5 glutathione peroxidase changes unit (U/ml)
| Group | Example number (example) | Before the test-meal | After the test-meal | Difference | Rising percentage rate % |
| Observation group's matched group | 50 50 | 79.03± 15.12 81.22± 16.58 | 89.95± 14.16 81.77± 13.97 | 10.93±19.74 ** ## 0.56±13.74 | 13.83 0.69 |
Compare before and after self
*##P＜0.01 is compared in P＜0.01 between group
(5) doing well,improving situation and symptom integral change
By table 6 as seen, marine collagen has the improvement effect to symptoms such as experimenter's feeling of fatigue, agitation, forgetful, sleep, soreness of waist and knee.By table 7 as seen, experimenter's test-meal mid-term, test-meal later stage than test-meal before symptom integral significantly reduce (P＜0.01).
Table 6 cardinal symptom is improved situation
| Cardinal symptom | The example number | Produce effects | Effectively | Invalid | Total effective rate |
| The forgetful soreness of waist and knee of the irritated sleep of feeling of fatigue | 29(34) 28(32) 18(22) 41(32) 40(31) | 2(0) 3(1) 1(1) 4(0) 4(1) | 13(12) 11(11) 9(6) 17(6) 16(5) | 14(22) 14(20) 8(15) 20(26) 20(25) | 51.72％(35.29％) 50.00％(37.50％) 55.56％(31.82％) 51.22％(18.75％) 50.00％(19.35％) |
In the bracket is matched group
Table 7 symptom integral changes
| Group | The example number | Before the test-meal | One month | Two months | After the test-meal |
| Observation group's matched group | 50 50 | 9.88±5.21 10.12±4.96 | 9.08±5.07 ** 9.66±4.92 ** | 8.38±4.86 ** 9.46±4.97 ** | 7.66±4.74 ** 9.24±4.96 ** |
Compare before and after self
*P＜0.01
(6) safety indexes detects:
By table 8 as seen, before and after the marine collagen test-meal, routine blood test, routine urinalysis, just routine and blood biochemistry index do not have significant change, point out product of the present invention to have no adverse effects to the experimenter is healthy.
Table 8 safety indexes detects
(8) Abdominal B type ultrasonography, electrocardiogram, the fluoroscopy of chest of X line is substantially in normal range.Blood pressure is not obvious before and after the test-meal.
2.5 conclusion
The volunteer took marine collagen after 90 days, and test-meal group lipid peroxide catabolite malonaldehyde obviously reduces, and contrast all has significant difference between own control and group; Superoxide dismutase raises to some extent, but contrasts there was no significant difference between own control and group; Glutathione peroxidase obviously raises, and contrast has significant difference between own control and group.Three indexs change all not obvious before and after the matched group test-meal.Illustrate that marine collagen can play anti-oxidation function by the generation that improves human body activities of antioxidant enzymes and reduction lipid peroxide.
In addition, marine collagen has the improvement effect to symptoms such as experimenter's feeling of fatigue, agitation, forgetful, sleep, soreness of waist and knee.Before and after the marine collagen test-meal, routine blood test, routine urinalysis, just routine and blood biochemistry index do not have significant change, and do not meet quick and other untoward reaction.Illustrate that this product has no adverse effects to the experimenter is healthy.
3. the marine collagen macula lutea function clinical observation experiment of dispelling
3.1 object and method
(1) tried thing: the marine collagen peptide capsule is provided by Zhejiang Hai Shi bio tech ltd.
(2) study subject: press the principle of voluntariness and select 18-65 year, chloasma is arranged, aspiration is tried to guarantee the crowd that cooperates.
1. diagnostic criteria: facial filbert to dark brown, well-defined patch, symmetry distributes usually, NIP performance and squama, subjective symptomss such as no sufferings.Certain seasonality is arranged, and Xia Chongdong is light.
2. do not include person's standard in: all volunteers that chloasma is arranged, all can carry out test-meal through medical fitness and observe.
3. eliminator's standard:
I. the age is under-18s or over-65s person, gestation or women breast-feeding their children, allergic constitution and to these health product allergy sufferers.
II. merge to have the inclination, serious primary disease and endocrinopathyes such as liver, kidney and hemopoietic system, the psychotic.
III. take the article relevant in a short time, have influence on judgement person the result with being tried function.
IV. eat in accordance with regulations and tried thing, can't judge effect or data not umbra sound effect or safety judgement person.
(3) contrast form: 100 routine chloasma experimenters, be divided into test-meal group and blank group at random, adopt two kinds of contrast forms between self and group.Keep on ordinary days life and dietary habit during being tried.
(4) taking dose and time: the test-meal group is taken marine collagen, and each 3, every day 3 times.The experimenter cuts out medicine or other health product and the cosmetics of relevant functions of removing chloasma at experimental session.
(5) instrument and reagent: MEK-6138K type blood counting instrument, MIDIRON urinates ten analysers, the RA1000 automatic clinical chemistry analyzer, Institute of Geography, Academia Sinica's development and design, Mapping Press published " practical standard colour atla " (front page) in 1992, and the biochemical reagents box is all provided by middle living company.
3.2 observation index: each is checked once before and after the experiment.
(1) safety indexes:
1. blood and urine, just routine examination:
Red blood cell count(RBC), hemoglobin, numeration of leukocyte is urinated ten detections, just conventional sense.
2. biochemical indicator is measured:
Serum albumin ALB, total protein TP, darling renal function (glutamic oxaloacetic transaminase, GOT AST, glutamate pyruvate transaminase ALT, carbamide UREA, creatinine CRE), blood glucose GLU, blood fat (T-CHOL TC, triglyceride TG).
3. Abdominal B type ultrasonography, electrocardiogram, the fluoroscopy of chest of X line.(checking once before the test-meal)
4. allergy and untoward reaction are observed:
(2) effect index:
1. general situation: spirit, sleep, diet, defecation, blood pressure etc.
2. observation of symptoms:
Detailed medical history-taking is understood experimenter's diet situation, observes main clinic symptoms: feeling of fatigue, agitation, forgetful, sleep, soreness of waist gonalgia etc.Before and after test-meal, add up integrated value by symptom weight (serious symptom 3 minutes, moderate 2 minutes, light disease 1 minute), and improve (2 fens produce effects of each doing well,improving are improved 1 and are divided into effectively), calculate the improvement rate with regard to its cardinal symptom.
3. women's head-ornaments portion chloasma detects
I. the area size changes: the major diameter and the wide footpath of same chloasma before and after being tried with tape measure, reference area (cm
2).
II. shade changes: according to Institute of Geography, Academia Sinica's development and design, brown (Y+M+BK i.e. Huang+magenta+black folded color) colour atla in " practical standard colour atla " (front page) that Mapping Press published in 1992 is the criterion of chloasma shade: I degree (15,20,5), II degree (30,40,10), III degree (40,60,15)
3.3 date processing and result judge
(1) date processing: with the STATE of statistical software 6.0 computational analysis data.All own control data adopt paired t-test, two groups of means relatively adopt t check in groups, and the latter need carry out homogeneity test of variance, and the data of nonnormal distribution or heterogeneity of variance are carried out suitable variable conversion, after waiting to satisfy the normal state homogeneity of variance, carry out the t check with data converted; If translation data still can not satisfy the requirement of normal state homogeneity of variance, use t ' check or rank test instead; But the coefficient of variation is too big as data (CV＞50%) is used rank test.The X 2 test of effect index.
(2) spot-removing function is judged
Effectively: chloasma color decline I degree, or area dwindle 〉=10%, do not produce new chloasma.Contrast all has significant difference between test-meal group self cross-reference and group.
Invalid: the chloasma area dwindles＜and 10%, color does not have significant change.
3.4 experimental result
(1) general situation
Observe experimenter's 100 examples altogether, spirit, sleep, diet, defecation, blood pressure all belong to normally.The test-meal group is the women, minimum 28 years old of age, maximum 65 years old, average 45.22 ± 10.08 years old, average course of disease 4.62 ± 3.01 years.Matched group is the women, minimum 21 years old of age, maximum 64 years old, average 45.40 ± 12.17 years old, average course of disease 4.66 ± 3.50 years.
(2) the chloasma shade changes
By table 9 as seen, after the test-meal group was taken marine collagen, the chloasma color shoaled, and colour atla on average reduces by 0.35 ± 0.38 degree (P＜0.01), and matched group chloasma color only shoals slightly, and colour atla on average reduces by 0.04 ± 0.17 degree.Contrast all has significant difference (P＜0.01) between test-meal group self cross-reference and group.
Table 9 chloasma shade changes
| Group | Example number (example) | Colour atla (degree) | ||
| Before the test-meal | After the test-meal | Difference | ||
| Test-meal group matched group | 50 50 | 2.00±0.34 2.09±0.37 | 1.65±0.51 2.05±0.34 | -0.35±0.38 **# -0.04±0.17 |
Compare before and after self
*##P＜0.01 is compared in P＜0.01 between group
(3) chloasma area size variation
By table 10 as seen, after the test-meal group was taken marine collagen, the chloasma area on average dwindled 4.75 ± 8.01 (P＜0.01), and matched group chloasma area on average dwindles 0.28 ± 1.46cm
2Contrast all has significant difference (P＜0.01) between test-meal group self cross-reference and group.
Table 10 chloasma area size variation
| Group | Example number (example) | Chloasma area (cm 2) | ||
| Before the test-meal | After the test-meal | Difference | ||
| Test-meal group matched group | 50 50 | 48.97±30.33 56.28±39.28 | 44.22±30.23 55.79±38.53 | -4.75±8.01 **# -0.28±1.46 |
Compare before and after self
*##P＜0.01 is compared in P＜0.01 between group
(4) effect evaluation
By table 11 as seen, after the test-meal group is taken marine collagen, chloasma treatment, effective 27 examples, total effective rate 54.00%, the treatment of matched group chloasma, effective 1 example, total effective rate 2.00%.Test-meal group and matched group relatively have significant difference (P＜0.01).
Table 11 effect is judged
| Group | Enabledisable | Total effective rate | |||
| The example number | Percentage ratio | The example number | Percentage ratio | ||
| Test-meal group matched group | 27 1 | 54.00％ 4.00％ | 23 49 | 46.00％ 96.00％ | 54.00％# 2.00％ |
Compare ##P＜0.01 between group
(5) doing well,improving and symptom integral situation of change
By table 12 as seen, marine collagen has the improvement effect to symptoms such as experimenter's feeling of fatigue, agitation, sleeps.By table 13 as seen, experimenter's test-meal mid-term, test-meal later stage than test-meal before symptom integral significantly reduce (P＜0.01), matched group also significantly reduces (P＜0.05).
Table 12 cardinal symptom is improved situation
| Cardinal symptom | The example number | Produce effects | Effectively | Invalid | Total effective rate |
| The irritated sleep of feeling of fatigue | 28(25) 34(23) 25(22) | 2(0) 3(0) 2(0) | 14(9) 16(6) 11(6) | 12(16) 15(17) 12(16) | 57.14％(36.00％) 55.88％(26.09％) 52.00％(27.27％) |
In the bracket is matched group
Table 13 symptom integral changes
| Group | The example number | Before the test-meal | In the test-meal | After the test-meal |
| Observation group's matched group | 50 50 | 3.98±2.55 3.72±3.28 | 3.44±2.44 ** 3.56±3.21 * | 2.98±2.12 ** 3.54±3.10 * |
Compare before and after self
*P＜0.05,
*P＜0.01
(7) safety indexes detects
By table 14 as seen, before and after the marine collagen test-meal, routine blood test, routine urinalysis, just routine and blood biochemistry index do not have significant change, point out product of the present invention to have no adverse effects to the experimenter is healthy.
Table 14 safety indexes detects
(8) Abdominal B type ultrasonography, electrocardiogram, the fluoroscopy of chest of X line is substantially in normal range.
3.5 conclusion
100 routine chloasma experimenters are divided into test-meal group and matched group at random, and test-meal group chloasma area dwindles, and color shoals, and the colour atla average degree reduces, indexs such as macula lutea treatment total effective rate, and contrast all has significant difference between test-meal group self cross-reference and group.Illustrate that marine collagen has the effect of functions of removing chloasma.
In addition, marine collagen has the improvement effect to symptoms such as chloasma experimenter's feeling of fatigue, agitation, sleeps.Before and after the marine collagen test-meal, routine blood test, routine urinalysis, just routine and blood biochemistry index be substantially in normal range, and do not meet quick and other untoward reaction, illustrates that this product has no adverse effects to the experimenter is healthy.
Claims (4)
1. a marine collagen is as the purposes for preparing anti-oxidation medicine, anti-oxidation health food, antioxidant food or antioxidation cosmetic product.
2. marine collagen according to claim 1 is as the purposes of preparation anti-oxidation medicine, anti-oxidation health food, antioxidant food or antioxidation cosmetic product, and it is characterized in that: described medicine, health food are powder, granule, capsule, tablet, pill or oral liquid.
3. marine collagen according to claim 1 is as the purposes of preparation anti-oxidation medicine, anti-oxidation health food, antioxidant food or antioxidation cosmetic product, and it is characterized in that: described food is starch-based product, breast or milk product, bean product or baking goods.
4. marine collagen according to claim 1 is as the purposes of preparation anti-oxidation medicine, anti-oxidation health food, antioxidant food or antioxidation cosmetic product, and it is characterized in that: described cosmetics are emulsion, cream frost, oil preparation, water preparation, gel, aerosol or facial film.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101576437A CN101168051A (en) | 2007-10-24 | 2007-10-24 | Application of sea collagen peptide as preparation of anti-oxidation medicine, health-care food, food or cosmetics |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101576437A CN101168051A (en) | 2007-10-24 | 2007-10-24 | Application of sea collagen peptide as preparation of anti-oxidation medicine, health-care food, food or cosmetics |
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| CN101168051A true CN101168051A (en) | 2008-04-30 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2007101576437A Pending CN101168051A (en) | 2007-10-24 | 2007-10-24 | Application of sea collagen peptide as preparation of anti-oxidation medicine, health-care food, food or cosmetics |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102198063A (en) * | 2011-05-27 | 2011-09-28 | 昆明理工大学 | Fish skin collagen peptide and olive oil compound skin-care product and preparation method thereof |
| CN102247589A (en) * | 2010-05-19 | 2011-11-23 | 李勇 | Application of marine collagen peptide in preparing medicament and food for delaying skin aging |
| CN104606683A (en) * | 2015-01-20 | 2015-05-13 | 北京福原环球生物科技有限公司 | Orally disintegrating preparation |
| CN107236774A (en) * | 2017-07-17 | 2017-10-10 | 青岛大学 | The peanut meal fermentation composition that a kind of anti-aging hypermnesia is recalled |
| CN116406798A (en) * | 2023-04-17 | 2023-07-11 | 云康大健康产业(广州)有限公司 | Marine bioactive peptide composition and application thereof |
-
2007
- 2007-10-24 CN CNA2007101576437A patent/CN101168051A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102247589A (en) * | 2010-05-19 | 2011-11-23 | 李勇 | Application of marine collagen peptide in preparing medicament and food for delaying skin aging |
| CN102198063A (en) * | 2011-05-27 | 2011-09-28 | 昆明理工大学 | Fish skin collagen peptide and olive oil compound skin-care product and preparation method thereof |
| CN102198063B (en) * | 2011-05-27 | 2013-05-08 | 昆明理工大学 | Fish skin collagen peptide and olive oil compound skin-care product and preparation method thereof |
| CN104606683A (en) * | 2015-01-20 | 2015-05-13 | 北京福原环球生物科技有限公司 | Orally disintegrating preparation |
| CN104606683B (en) * | 2015-01-20 | 2017-08-01 | 北京福原环球生物科技有限公司 | A kind of oral disintegrated preparation |
| CN107236774A (en) * | 2017-07-17 | 2017-10-10 | 青岛大学 | The peanut meal fermentation composition that a kind of anti-aging hypermnesia is recalled |
| CN116406798A (en) * | 2023-04-17 | 2023-07-11 | 云康大健康产业(广州)有限公司 | Marine bioactive peptide composition and application thereof |
| CN116406798B (en) * | 2023-04-17 | 2023-10-03 | 云康大健康产业(广州)有限公司 | Marine bioactive peptide composition and application thereof |
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Open date: 20080430 |