CN101156552A - Oriental lily tissue culture detoxification method - Google Patents
Oriental lily tissue culture detoxification method Download PDFInfo
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- CN101156552A CN101156552A CNA2007100663391A CN200710066339A CN101156552A CN 101156552 A CN101156552 A CN 101156552A CN A2007100663391 A CNA2007100663391 A CN A2007100663391A CN 200710066339 A CN200710066339 A CN 200710066339A CN 101156552 A CN101156552 A CN 101156552A
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- 241000234435 Lilium Species 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000001784 detoxification Methods 0.000 title abstract description 11
- 239000001963 growth medium Substances 0.000 claims abstract description 26
- 238000005286 illumination Methods 0.000 claims description 21
- 150000003839 salts Chemical class 0.000 claims description 11
- 229920001817 Agar Polymers 0.000 claims description 6
- 229930006000 Sucrose Natural products 0.000 claims description 6
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 239000002609 medium Substances 0.000 claims description 6
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 claims description 6
- 239000005720 sucrose Substances 0.000 claims description 6
- 230000003203 everyday effect Effects 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000004140 cleaning Methods 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 230000001939 inductive effect Effects 0.000 abstract 1
- 241000700605 Viruses Species 0.000 description 20
- 241000724252 Cucumber mosaic virus Species 0.000 description 8
- 230000008030 elimination Effects 0.000 description 5
- 238000003379 elimination reaction Methods 0.000 description 5
- 239000002574 poison Substances 0.000 description 4
- 231100000614 poison Toxicity 0.000 description 4
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000008676 import Effects 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000008774 maternal effect Effects 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000763 evoking effect Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 1
- 229960000367 inositol Drugs 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 210000004914 menses Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 238000002161 passivation Methods 0.000 description 1
- 238000004161 plant tissue culture Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- 238000002054 transplantation Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a tissue culture detoxification method of oriental lily, which comprises the steps of selecting healthy lily scales, cutting, inducing adventitious buds, transferring the adventitious buds to a growth culture medium, carrying out variable temperature culture for 30 days, cutting stem tips, transplanting the stem tips to the growth culture medium, and continuing the variable temperature culture for 30-60 days to obtain a required non-toxic plant line. The invention has obvious detoxification effect and high efficiency, and the general variety can reach 90-100%. The method is simple and convenient to operate, low in cost and good in application prospect.
Description
Technical field
The invention belongs to field of plant growing technology, be specifically related to a kind of method of oriental lily tissue culture detoxicating.
Background technology
Oriental hybrid lily (Lilium spp.cv.oriental hybrids) is a hybridization strain in the lilium, is one of flowering bulb most popular on the international market.Because the great demand in market, China is maternal from external a large amount of import oriental hybrid lily splits and plantation every year, is example with 2006 only, about 7,000 ten thousand of import split, maternal about 8,000,000 of plantation.Not only expend a large amount of foreign exchanges, and increased the operating cost and the risk of enterprise.
At present, the artificial culture of oriental hybrid lily seedling all adopts conventional method for plant tissue culture, promptly is explant with the scale, and first evoked callus makes callus differentiate root and stem after the switching, and then cultivates a bottle seedling.If but explant is malicious in spite of illness, then this method can only reduce the viral level of seedling, is difficult to make seedling thoroughly to remove virus.Virus can accumulate in bulb is planted year by year, causes the degeneration of kind of ball quality and merit.Simultaneously, also must be after the bottle transplantation of seedlings through white silk the seedling process, so that seedling slowly adapts to the transplanting environment.This moment, the survival rate of seedling depended primarily on the quality and the gerentocratic fine degree of seedling-exercising appliance, not only the production cycle long, and increased the cost of production management.According to report, there are 12 kinds of viruses can endanger the oriental hybrid lily growth at present, wherein lily mosaic virus (LiMV), lily asymptomatic virus (LSV) and cucumber mosaic virus (CMV) are to endanger the most serious virus, if take off this three kinds of viruses, just can guarantee the quality of lily substantially.The detoxification treatment of relevant lily all is to adopt stem apex, medicine or heat treatment method separately both at home and abroad at present, and these method efficient are low, and detoxification efficiency is poor, and virus elimination rate is below 30% mostly.Take the strain bud after 40 minutes, to cut the method for stem apex with hot water (50 ± 1) ℃ processing in recent years, though obtained certain effect, the tissue culture ball is after handling 40 minutes under 50 ℃ the high temperature, and its survival rate is lower; Simultaneously because the bulbil non-sterile behind thermal treatment and excision stem apex, also is difficult to guarantee not contaminated.Up to now, do not see the method report that can effectively overcome the problems referred to above in the prior art as yet.
Summary of the invention
The objective of the invention is at the deficiencies in the prior art, provide a kind of with low cost, obvious results oriental lily tissue culture detoxicating method.
Purpose of the present invention is achieved by the following technical programs.
A kind of method of oriental lily tissue culture detoxicating, this method adopts following steps:
1, selects the oriental hybrid lily plant of robust growth at later stages, take out bulb, divide scale down from bulb, and with after clear water cleaning, the sterilization, under aseptic condition, the scale branch is cut to 3～5, the scale that cuts is placed take place to carry out aseptic culture on the medium, setting cultivation temperature and be 22 ℃～25 ℃, intensity of illumination is 1000lx～4000lx, cultivates and induces indefinite bud after 60 days;
2, indefinite bud is transferred on the growth medium, alternating temperature was cultivated 30 days in the artificial climate incubator, described alternating temperature condition of culture is every day in temperature is that 37 ℃, intensity of illumination are to cultivate 12 hours under the condition of 1000lx～4000lx, in temperature is to cultivate 12 hours under 22 ℃, dark surrounds, and both hocket;
3, through after the alternating temperature processing in 30 days, under aseptic condition, cut 0.8～1.0mm stem apex and be transplanted to continuation alternating temperature cultivation in the growth medium, condition of culture is that illumination/dark respectively hocketed in 12 hours, during illumination, cultivation temperature is that 22 ℃～25 ℃, intensity of illumination are 1000lx～4000lx; When dark, temperature is 22 ℃～25 ℃; Cultivate after 30～60 days, just can obtain required avirulent strain system.
Described generation medium is for containing methyl (NAA) 0.05～0.1mg/L, contain sucrose 7%～9%, containing the high salt culture medium of MS or the high salt culture medium of 1/2MS of agar 0.65%.
Described growth medium is for containing methyl (NAA) 0.05～0.1mg/L, contain sucrose 7%～9%, containing the high salt culture medium of 1/2～1/5MS of agar 0.65%.
Compared with prior art, the present invention has following advantage:
1. by guarantee the growth of tissue culture ball under the alternating temperature treatment conditions, the virus of passivation simultaneously reduces virus and transfers to stem apex, and the assurance growing point is not with poison, cuts 0.8～1.0mm Shoot Tip Culture then, arrives the purpose of detoxification.
2. detoxification efficiency is remarkable, the efficient height, and general kind can reach 90%～100%.
Embodiment
In order to understand essence of the present invention better, the invention will be further described below in conjunction with embodiment and experimental example, but they are not limitations of the present invention.
Embodiment 1
Select the oriental hybrid lily plant of robust growth in September, take out bulb, divide scale down from bulb, and with after clear water cleaning, the sterilization, under aseptic condition, scale is cut into 3～5 little fast, the scale that cuts placed take place to carry out aseptic culture on the medium, setting cultivation temperature and be 23.5 ℃, intensity of illumination is 2000～3000lx, cultivates and induces indefinite bud after 60 days; Indefinite bud is transferred on the growth medium, alternating temperature was cultivated 30 days in the artificial climate incubator, described alternating temperature is cultivated to cultivating 12 hours under temperature is 37 ℃, intensity of illumination 2000～3000lx respectively every day, cultivates 12 hours under temperature is 22 ℃, dark surrounds, and both hocket; After alternating temperature processing in 30 days, under aseptic condition, cut the 0.8mm stem apex, be transplanted to and continue alternating temperature cultivation 30～60 days in the growth medium, just can obtain required avirulent strain system.Condition of culture be illumination/dark each hocketed in 12 hours, during illumination, cultivation temperature is that 22 ℃～25 ℃, intensity of illumination are 1000lx～4000lx; When dark, temperature is 22 ℃～25 ℃.
Wherein said generation medium is for containing methyl (NAA) 0.05～0.1mg/L, contain sucrose 7%～9%, containing the high salt culture medium of MS or the high salt culture medium of 1/2MS of agar 0.65%.
Described growth medium is for containing methyl (NAA) 0.05～0.1mg/L, contain sucrose 7%～9%, containing the high salt culture medium of 1/2～1/5MS of agar 0.65%.
Described MS is high, and the salt culture medium prescription sees attached list 1
The high salt culture medium prescription of table 1 MS
| Composition | Content (mg/L) |
| 1. macroelement | |
| ?NH 4NO 3 | ?1650 |
| ?KNO 3 | ?1900 |
| ?CaCl 2·2H 2O | ?440 |
| ?MgSO 4·7H 2O | ?370 |
| ?KH 2PO 4 | ?170 |
| 2. micro- | |
| ?FeSO 4·7H 2O | ?27.8 |
| ?Na 2·EDTA | ?37.3 |
| ?KI | ?0.83 |
| ?H 3BO 3 | ?6.2 |
| ?MnSO 4·4H 2O | ?22.3 |
| ?ZnSO 4·7H 2O | ?8.6 |
| ?Na 2·MoO 4·2H 2O | ?0.25 |
| ?CuSO 4·5H 2O | ?0.025 |
| ?CoCl 2·6H 2O | ?0.025 |
| 3. organic component | |
| Inositol | 100 |
| Nicotinic acid | 0.5 |
| Puridoxine hydrochloride | 0.5 |
| Thiamine hydrochloride | 0.1 |
| Glycine | 2.0 |
Embodiment 2
Repeat embodiment 1, following difference is arranged: divide the scale that cuts to place under 22 ℃ of conditions and cultivate.
Embodiment 3
Repeat embodiment 1, following difference is arranged: divide the scale that cuts to place under 25 ℃ of conditions and cultivate.
Embodiment 4
Repeat embodiment 1, following difference is arranged: divide the scale that cuts to place under intensity of illumination 1000～2000lx condition and cultivate.
Embodiment 5
Repeat embodiment 1, following difference is arranged: divide the scale that cuts to place under intensity of illumination 3000～4000lx condition and cultivate.
Embodiment 6
Repeat embodiment 1, following difference is arranged: indefinite bud is transferred on the growth medium, alternating temperature was cultivated 30 days in the artificial climate incubator, and described alternating temperature condition of culture is every day in temperature is that 37 ℃, intensity of illumination are to cultivate 12 hours under the condition of 1000lx～2000lx; In temperature is to cultivate 12 hours under 22 ℃, dark surrounds, and both hocket.
Embodiment 7
Repeat embodiment 1, following difference is arranged: indefinite bud is transferred on the growth medium, alternating temperature was cultivated 30 days in the artificial climate incubator, and described alternating temperature condition of culture is every day in temperature is that 37 ℃, intensity of illumination are to cultivate 12 hours under the condition of 3000lx～4000lx; In temperature is to cultivate 12 hours under 22 ℃, dark surrounds, and both hocket.
Embodiment 8
Repeat embodiment 1, following difference is arranged: indefinite bud under aseptic condition, cuts the 1.0mm stem apex after handling through 30 days alternating temperatures, is transplanted to alternating temperature cultivation in the growth medium.
Embodiment 9
Repeat embodiment 1, following difference is arranged: indefinite bud under aseptic condition, cuts the 0.9mm stem apex after handling through 30 days alternating temperatures, is transplanted to alternating temperature cultivation in the growth medium.
Experimental example
(1) material: for real kind is Sol nation (Sorbone), the Supreme Being dials (Tiber) and three kinds of northwest Leah (Siberia), and the cleer and peaceful Molecular Detection of menses has the kind ball of lily mosaic virus (LiMV), lily asymptomatic virus (LSV) and cucumber mosaic virus (CMV) respectively.
(2) method: pressing embodiment 1 described method will be with the kind ball of poison, cultivate by scale, obtain the tissue culturing seedling of band poison, the cultivation ball of band poison is transferred in the new blake bottle, 5 every bottle, every kind of virus switching kinds 5 bottles, wherein 1 bottle is placed on 22 ℃ in contrast and cultivated in hour dark of illumination/12 in 12 hours.All the other 4 bottles are placed in the climatic cabinate and cultivated 30 days, and between culture period, design temperature was changed to 37 ℃, illumination cultivation 12 hours, 22 ℃, dark culturing 12 hours, after cultivating January, cut 0.8～1.0mm stem apex and cultivate under aseptic condition, cultivation is carried out virus and is detected after February.
(3) result:
Table 1: virus elimination rate after the detoxification treatment
| Kind | Detoxification treatment quantity | Nontoxic quantity after the detoxification treatment | Virus elimination rate | Contrast quantity | Be with malicious number after the cultivation | Be with malicious rate |
| The band LiMV of Sol nation | 4 bottles (20) | 20 | ?100％ | 1 bottle (5) | 5 | ?100％ |
| The band LSV of Sol nation | 4 bottles (20) | 20 | ?100％ | 1 bottle (5) | 5 | ?100％ |
| The band CMV of Sol nation | 4 bottles (20) | 19 | ?95％ | 1 bottle (5) | 5 | ?100％ |
| The Supreme Being dials LiMV | 4 bottles (20) | 20 | ?100％ | 1 bottle (5) | 5 | ?100％ |
| The Supreme Being dials LSV | 4 bottles (20) | 20 | ?100％ | 1 bottle (5) | 5 | ?100％ |
| The Supreme Being dials CMV | 4 bottles (20) | 19 | ?95％ | 1 bottle (5) | 5 | ?100％ |
| Leah northwest, northwest Leah LiMV | 4 bottles (20) | 20 | ?100％ | 1 bottle (5) | 5 | ?100％ |
| Northwest Leah LSV | 4 bottles (20) | 20 | ?100％ | 1 bottle (5) | 5 | ?100％ |
| Northwest Leah CMV | 4 bottles (20) | 18 | ?90％ | 1 bottle (5) | 5 | ?100％ |
The result shows, tissue culture ball warp alternating temperature was handled after 30 days, the detoxification efficiency that cuts 0.8～1.0mm Shoot Tip Culture is best, and lily mosaic virus (LiMV), lily asymptomatic virus (LSV) virus elimination rate reach 100%, and cucumber mosaic virus (CMV) virus elimination rate reaches more than 90%.
Claims (3)
1. the method for an oriental lily tissue culture detoxicating, this method adopts following steps:
(1) selects the oriental hybrid lily plant of robust growth at later stages, take out bulb, divide scale down from bulb, and with after clear water cleaning, the sterilization, under aseptic condition, the scale branch is cut to 3～5, the scale that cuts is placed take place to carry out aseptic culture on the medium, setting cultivation temperature and be 22 ℃～25 ℃, intensity of illumination is 1000lx～4000lx, cultivates and induces indefinite bud after 60 days;
(2) indefinite bud is transferred on the growth medium, alternating temperature was cultivated 30 days in the artificial climate incubator, described alternating temperature condition of culture is every day in temperature is that 37 ℃, intensity of illumination are to cultivate 12 hours under the condition of 1000lx～4000lx, in temperature is to cultivate 12 hours under 22 ℃, dark surrounds, and both hocket;
(3) through after the alternating temperature processing in 30 days, under aseptic condition, cut 0.8～1.0mm stem apex and be transplanted to continuation alternating temperature cultivation in the growth medium, condition of culture is that illumination/dark respectively hocketed in 12 hours, during illumination, cultivation temperature is that 22 ℃～25 ℃, intensity of illumination are 1000lx～4000lx; When dark, temperature is 22 ℃～25 ℃; Cultivate after 30～60 days, just can obtain required avirulent strain system.
2. poison-removing method according to claim 1 is characterized in that: described generation medium is for containing methyl (NAA) 0.05～0.1mg/L, contain sucrose 7%～9%, containing the high salt culture medium of MS or the high salt culture medium of 1/2MS of agar 0.65%.
3. poison-removing method according to claim 1 is characterized in that: described growth medium is for containing methyl (NAA) 0.05～0.1mg/L, contain sucrose 7%～9%, containing the high salt culture medium of 1/2～1/5MS of agar 0.65%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100663391A CN101156552B (en) | 2007-11-02 | 2007-11-02 | Oriental lily tissue culture detoxicating method |
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| CN2007100663391A CN101156552B (en) | 2007-11-02 | 2007-11-02 | Oriental lily tissue culture detoxicating method |
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| CN101156552A true CN101156552A (en) | 2008-04-09 |
| CN101156552B CN101156552B (en) | 2010-06-02 |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102440184A (en) * | 2010-10-14 | 2012-05-09 | 王文和 | Culturing method for utilizing bioreactor to quickly expand lily detoxified cormels |
| CN102440185A (en) * | 2010-10-14 | 2012-05-09 | 赵祥云 | Fast culturing method for lily virus-free seed ball |
| CN103688864A (en) * | 2013-12-18 | 2014-04-02 | 杭州市园林绿化股份有限公司 | Lilium oriental hybrid tissue culture seedling strengthening method |
| CN103688861A (en) * | 2013-12-18 | 2014-04-02 | 浙江诚邦园林股份有限公司 | Lilium oriental hybrid tissue culture seedling strengthening method |
| CN103798141A (en) * | 2014-01-26 | 2014-05-21 | 浙江大学 | Method for establishing lily embryogenic callus regeneration system by using pedicles as explants |
| CN106035086A (en) * | 2016-06-15 | 2016-10-26 | 张万萍 | Detoxification tissue culture and rapid propagation method of lily |
| CN107258540A (en) * | 2017-07-26 | 2017-10-20 | 株洲市农业科学研究所 | The tissue culture method of fast breeding oriental hybrid lily hybrid new breed |
| CN109220792A (en) * | 2018-09-28 | 2019-01-18 | 山东省农业科学院生物技术研究中心 | A kind of fast numerous method of iris stem apex detoxification regeneration |
| CN111280059A (en) * | 2020-03-26 | 2020-06-16 | 南京农业大学 | Efficient lily detoxification method |
| CN113170731A (en) * | 2021-05-26 | 2021-07-27 | 长江师范学院 | High-temperature detoxification method based on lily bulb scale leaf tips and application thereof |
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| CN100394845C (en) * | 2004-10-21 | 2008-06-18 | 云南省农业科学院花卉研究所 | In-bottle production method of detoxified small seed ball of east lily |
| CN100333634C (en) * | 2005-06-17 | 2007-08-29 | 云南格桑花卉有限责任公司 | Method for cultivating oriental lily test tube bulb |
| CN1903016A (en) * | 2005-07-29 | 2007-01-31 | 潘利军 | Virus removing method for lily tissue culturing seedling by heat treatment |
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- 2007-11-02 CN CN2007100663391A patent/CN101156552B/en not_active Expired - Fee Related
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| CN102440185B (en) * | 2010-10-14 | 2015-05-27 | 赵祥云 | Fast culturing method for lily virus-free seed ball |
| CN102440185A (en) * | 2010-10-14 | 2012-05-09 | 赵祥云 | Fast culturing method for lily virus-free seed ball |
| CN102440184A (en) * | 2010-10-14 | 2012-05-09 | 王文和 | Culturing method for utilizing bioreactor to quickly expand lily detoxified cormels |
| CN103688864B (en) * | 2013-12-18 | 2015-11-04 | 杭州市园林绿化股份有限公司 | The method in a kind of oriental hybrid lily plantlet in vitro strong sprout |
| CN103688861A (en) * | 2013-12-18 | 2014-04-02 | 浙江诚邦园林股份有限公司 | Lilium oriental hybrid tissue culture seedling strengthening method |
| CN103688864A (en) * | 2013-12-18 | 2014-04-02 | 杭州市园林绿化股份有限公司 | Lilium oriental hybrid tissue culture seedling strengthening method |
| CN103688861B (en) * | 2013-12-18 | 2016-01-20 | 浙江诚邦园林股份有限公司 | The method in a kind of oriental hybrid lily plantlet in vitro strong sprout |
| CN103798141A (en) * | 2014-01-26 | 2014-05-21 | 浙江大学 | Method for establishing lily embryogenic callus regeneration system by using pedicles as explants |
| CN103798141B (en) * | 2014-01-26 | 2016-03-30 | 浙江大学 | A kind of take bennet as the method that explant sets up lily embryo callus subculture regenerating system |
| CN106035086A (en) * | 2016-06-15 | 2016-10-26 | 张万萍 | Detoxification tissue culture and rapid propagation method of lily |
| CN107258540A (en) * | 2017-07-26 | 2017-10-20 | 株洲市农业科学研究所 | The tissue culture method of fast breeding oriental hybrid lily hybrid new breed |
| CN109220792A (en) * | 2018-09-28 | 2019-01-18 | 山东省农业科学院生物技术研究中心 | A kind of fast numerous method of iris stem apex detoxification regeneration |
| CN111280059A (en) * | 2020-03-26 | 2020-06-16 | 南京农业大学 | Efficient lily detoxification method |
| CN113170731A (en) * | 2021-05-26 | 2021-07-27 | 长江师范学院 | High-temperature detoxification method based on lily bulb scale leaf tips and application thereof |
| CN113170731B (en) * | 2021-05-26 | 2022-11-22 | 长江师范学院 | High-temperature detoxification method based on lily bulb scale leaf tips and application thereof |
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| Publication number | Publication date |
|---|---|
| CN101156552B (en) | 2010-06-02 |
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