CN1903016A - Virus removing method for lily tissue culturing seedling by heat treatment - Google Patents
Virus removing method for lily tissue culturing seedling by heat treatment Download PDFInfo
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- CN1903016A CN1903016A CN 200510046955 CN200510046955A CN1903016A CN 1903016 A CN1903016 A CN 1903016A CN 200510046955 CN200510046955 CN 200510046955 CN 200510046955 A CN200510046955 A CN 200510046955A CN 1903016 A CN1903016 A CN 1903016A
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- lily
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Abstract
A heat treating method for detoxicating the tissue-cultured seedlings of lily features that the lily bulb in germination state is treated at 35-50 deg.C and 100% air humidity for 20-40 days. Its advantages are high survival rate and high detoxicating effect.
Description
Technical field
The present invention relates to Plant Tissue Breeding, the method for the detoxification of tissue-cultured derived plant lily thermal treatment specifically.
Background technology
Some important flowers, ornamental plants in garden can be bred virus-free seedling in a large number with the method for tissue culture.Large quantities of flowers such as chrysanthemum, carnation, sword lily, narcissus, tulip, lily, dahlia, Spathiphyllum kochii are by the asexual process breeding, and virus is transmitted accumulation by generation, and harm is on the rise.Be with malicious plant virus concentration after high temperature treatment to reduce, active meeting passivation, thermal treatment generally is to carry out in hot-air, pending plant material is placed under 35～40 ℃ the high temperature, different according to the plant kind with the situation of plant material, processing time is not waited from several days by tens days, even the plant that has can handle tens days, and it is still survived.
Lily flower is by the asexual process breeding, and virus is also being transmitted accumulation by generation, and does not also have play-by-play for the Technology for Heating Processing of lily; Mention flowering bulbs such as (P194) lily, tulip, hyacinth in Xiong Li and Wu Lifang chief editor " tissue culture of ornamental flower and large-scale production ", heat-treat, can reduce the viral level in kind of the ball growing point with dormancy kind ball; But to how lily do not heat-treated and to mention, how to guarantee higher survival rate and preferably detoxification efficiency be heat treated key.
Summary of the invention
The object of the present invention is to provide the method for a kind of survival rate height, tissue-cultured derived plant lily thermal treatment detoxification that detoxification efficiency is good.
For achieving the above object, the technical solution used in the present invention is:
The method of tissue-cultured derived plant lily thermal treatment detoxification will be in lily bulb under the state of sprouting (refer to non-resting state or breaking dormancy state) at 35～50 ℃ high temperature, handle under the situation of air humidity 100% 20～40 days.
Described temperature is preferably 40～45 ℃, and the processing time is preferably 25～35 days.
Advantage of the present invention is as follows:
1. survival rate height.Survival rate is more than 91% in the heat treatment process of the present invention, and survival rate is higher.
2. detoxification efficiency is good.The lily bulb virus elimination rate is more than 76.5% after the thermal treatment of the present invention, and virus elimination rate is higher.
3. effect is good.The heat treated lily bulb of the present invention is the non-resting state or the lily bulb of breaking dormancy state, can be directly used in the group training after the thermal treatment and produce, and has shortened the time of lily tissue culture and inducement Cheng Miao; After the virus removal, the plant strain growth gesture is strong, and it is big that flower becomes, lovely luster, and anti-adversity ability improves, and produces flower quantity and rises.
Embodiment
The method of embodiment 1 tissue-cultured derived plant lily thermal treatment detoxification:
1) 1600 in the generation kind ball that will be in the imported from Holland of the state of sprouting places and carries out following several groups of thermal treatments in the insulating box: air humidity 100% in the control insulating box, and the processing time is 30 days, observes its survival rate and detoxification efficiency;
Table 1
| Temperature (℃) | 24 | 35 | 40 | 42 | 44 | 45 | 50 | 55 |
| Grain number (individual) | 200 | 200 | 200 | 200 | 200 | 200 | 200 | 200 |
| Survival grain number (individual) | 200 | 191 | 187 | 186 | 183 | 182 | 89 | 0 |
| Survival rate % | 100 | 95.5 | 93.5 | 93 | 91.5 | 91 | 44.5 | 0 |
| Anosis virion number (individual) | 97 | 103 | 143 | 155 | 169 | 171 | 85 | |
| Virus-free rate % | 48.5 | 53.9 | 76.5 | 83.3 | 92.3 | 94.0 | 95.5 |
As can be seen from the above table: when temperature raise in 35～55 ℃ interval gradually: survival rate descended gradually, and when temperature reached 50 ℃, survival rate was below 50%; The virus-free rate then improves gradually, and when temperature reached 40 ℃, the virus-free rate was 76.5%; Comprehensive above-mentioned two factors, treatment temperature is 40～45 ℃ preferably.
And grope to learn that the lily heat treatment time of the state of sprouting is relevant with the bulb size with the kind of lily from the experience of reality, its processing time can be 20～40 days usually, is preferably 25～35 days, is preferably 28-32 days.
Claims (3)
1. the method for tissue-cultured derived plant lily thermal treatment detoxification, it is characterized in that: the lily bulb that will be under the state of sprouting was handled 20～40 days under the situation of 35～50 ℃ of temperature, air humidity 100%.
2. according to the method for the described tissue-cultured derived plant lily thermal treatment of claim 1 detoxification, it is characterized in that: described temperature is 40～45 ℃.
3. according to the method for the described tissue-cultured derived plant lily thermal treatment of claim 1 detoxification, it is characterized in that: 25～35 days described processing times.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510046955 CN1903016A (en) | 2005-07-29 | 2005-07-29 | Virus removing method for lily tissue culturing seedling by heat treatment |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510046955 CN1903016A (en) | 2005-07-29 | 2005-07-29 | Virus removing method for lily tissue culturing seedling by heat treatment |
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| CN1903016A true CN1903016A (en) | 2007-01-31 |
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| CN 200510046955 Pending CN1903016A (en) | 2005-07-29 | 2005-07-29 | Virus removing method for lily tissue culturing seedling by heat treatment |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156552B (en) * | 2007-11-02 | 2010-06-02 | 云南格桑花卉有限责任公司 | Oriental lily tissue culture detoxicating method |
| CN102870676A (en) * | 2012-09-18 | 2013-01-16 | 龙山县生物科技开发公司 | Longshan lily high-efficient detoxification process |
| CN104429942A (en) * | 2014-11-03 | 2015-03-25 | 中国农业科学院果树研究所 | Detoxification method combining heat treatment and chemical treatment for apple tissue culture seedlings |
| CN105493972A (en) * | 2015-12-04 | 2016-04-20 | 中国农业科学院柑桔研究所 | Method for efficiently removing CTLV (citrus tatter leaf virus) |
-
2005
- 2005-07-29 CN CN 200510046955 patent/CN1903016A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101156552B (en) * | 2007-11-02 | 2010-06-02 | 云南格桑花卉有限责任公司 | Oriental lily tissue culture detoxicating method |
| CN102870676A (en) * | 2012-09-18 | 2013-01-16 | 龙山县生物科技开发公司 | Longshan lily high-efficient detoxification process |
| CN104429942A (en) * | 2014-11-03 | 2015-03-25 | 中国农业科学院果树研究所 | Detoxification method combining heat treatment and chemical treatment for apple tissue culture seedlings |
| CN105493972A (en) * | 2015-12-04 | 2016-04-20 | 中国农业科学院柑桔研究所 | Method for efficiently removing CTLV (citrus tatter leaf virus) |
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Open date: 20070131 |