CN101147463A - Polysiphonia urceolata sporophyte direct seedling formation method - Google Patents
Polysiphonia urceolata sporophyte direct seedling formation method Download PDFInfo
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- CN101147463A CN101147463A CNA2007101130939A CN200710113093A CN101147463A CN 101147463 A CN101147463 A CN 101147463A CN A2007101130939 A CNA2007101130939 A CN A2007101130939A CN 200710113093 A CN200710113093 A CN 200710113093A CN 101147463 A CN101147463 A CN 101147463A
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- alga
- sporophyte
- algae
- frond
- alga body
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
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- Cultivation Of Seaweed (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a polysiphonous alga sporophyle direct seedling-forming method. It is characterized by that said method includes the following steps: selecting health alga body from wild population, using firstly-boiled lately-cooled sea water to wash the alga body until the heterogeneous alga is removed, then cleaning said alga body for 3-5 times by using ultrasonic wave until on the alga body the heterogeneous alga pollution is completely eliminated, using pulverizing machine to pulverize alga body, filtering said pulverized alga body by using screen cloth whose pore size is 120-130 micron, then making the obtained filtrate be passed through screen cloth whose pore size is 90-100 micron, culturing the obtained alga part in culture tank with 10-15 deg.C, light intensity is 2000-3000 lux, illumination time is 10-14hr, after 3-7 days, enough number of pseudorhizae and innovations with enough length can be grown on the alga part.
Description
Technical field
The present invention relates to a kind of method of polysiphonia urceolata sporophyte direct seedling formation.
Background technology
Polysiphonia is a class red algae of the true Rhodophyceae Rhodomelaceae of Rhodophyta.Studies show that in recent years, the multitube algae has antibacterium and fungi, anti-herpesvirus and antioxidation activity, and multiple cancer cell (as colon cancer cell, neurinoma cell) had cytotoxic activity, so have important DEVELOPMENT PROSPECT aspect the marine alga drug development; In addition, also contain materials such as abundant sulfuric acid galactose, phycoerythrin in the multitube algae, important economic value is also arranged.Excellent development utilizes prospect to make the natural resources of multitube algae can not satisfy potential exploitation needs, is prospective way so development can be used for the method for artificial cultivation production, carries out tame relevant report but have not yet to see the multitube algae.
Summary of the invention
The method that the purpose of this invention is to provide a kind of polysiphonia urceolata sporophyte direct seedling formation, it can satisfy the demand of prior art.
A kind of method of polysiphonia urceolata sporophyte direct seedling formation is characterized in that gathering target sporophyte individuality from wild population, chooses healthy frond, scrubs several times with the seawater that boils the back cooling earlier, up to there not being significantly assorted algae; Clean 3-5 time with ultrasonic then, to the frond till the assorted algae pollution, with beating crusher frond is smashed again, by the aperture is the silk cover filtering of 120-130 μ m, and making filtrate is the bolting silk of 90-100 μ m by the aperture, and the algae section that obtains is cultivated in 10-15 ℃ of incubator, light intensity is 2000-3000lux, light application time is 10-14 hour, and through 3-7 days, the algae section just can grow the rhizoid and the innovation of sufficient amount and length.
Advantage of the present invention is: 1, the multitube algae is sporophyte and gametophyte isomorphic alternation of generations, and the carpospore that true Rhodophyceae arranged from generation to generation, adopt sporophyte directly as the seedling source, not only avoided amphigenetic complex process and very long cultivation period, and kept parent's genetic character; 2, it is short, effective to clean required time with ultrasonic; 3, filter by substep, the algae section is controlled at certain-length, be beneficial to the density of control seedling, can reduce the winding between the long algal filament and influence the uniformity of seedling; 4, the growth conditions of the rhizoid of algae section and shoot is approaching, is easy to the seedling that secures good health in the production.
Embodiment
The inventor is in 3-4 in 2007 between the month, the polysiphonia urceolata sporophyte that picks up from the beach, Qingdao taken back indoor, therefrom chooses healthy frond.In the seawater that boils the back cooling, scrub to not having significantly assorted algae and attachment with cotton balls; At this moment, often also have a spot of assorted algae such as diatom etc. attached on the frond, in a single day such frond gives suitable condition breeding fast, influences the growth of multitube algae.In order further to clean frond, the polysiphonia urceolata sporophyte of scrubbing is immersed in the clean seawater, clean repeatedly with ultrasonic cell disruption instrument, power is generally 150 watts, each 10 seconds, can not overlong time, in order to avoid heat production too much causes damage to frond, all assorted algaes can be removed general 3-5 time, but clean seawater will be changed after each the cleaning.Till microscopy determines that the assorted algae of nothing adheres to.Take the frond of wash clean according to aequum, immerse in an amount of clean seawater, frond is smashed, do not beat for a long time when noting smashing continuously, preferably make a call to after 10 seconds and suspend, in order to avoid heat production damages algal filament with beating crusher.After having beaten,, filter for the algal filament section that obtains certain-length is used for inoculation.Crossing the aperture earlier is the bolting silk of 120-130 μ m, collects filtrate, and crossing the aperture then is the bolting silk of 90-100 μ m, and the algal filament of collecting on the bolting silk is that length is the algal filament of 90-130 μ m.Some are beneficial to the regeneration of rhizoid and shoot the algal filament weak point; And the branch middle part is not easy to interrupt, so general length is longer, simultaneously because the short easier regeneration in branch end is cultivated easier acquisition regrowth so get short end in principle as far as possible.The algal filament that obtains is scattered in a certain amount of clean seawater, under the even stirring of agitator, is inoculated in the culture dish, can add attaching substratum such as vinylon rope etc. in advance in the cultivation.It is full that the culture dish that inoculation is good adds clean seawater to 2/3, leaves standstill a period of time (about 20 minutes) to algal filament and sink, and is put into gently then and cultivates in the incubator, and condition of culture is 10-15 ℃, light intensity 2000-3000lux, light application time 10-14 hour.After 3-7 days, promptly have rhizoid and innovation to grow, algal filament begins to adhere to, and the clean seawater that at this moment can change Ensure Liquid is to accelerate the growth rate of algal filament.After one week, every section algal filament has substantially all grown several rhizoids and attached on the matrix or culture dish bottom, firmly degree can reach production requirement, can plunge into the commercial sea the opposing stormy waves.
Claims (1)
1. the method for a polysiphonia urceolata sporophyte direct seedling formation is characterized in that gathering target sporophyte individuality from wild population, chooses healthy frond, scrubs several times with the seawater that boils the back cooling earlier, up to there not being significantly assorted algae; Clean 3-5 time with ultrasonic then, to the frond till the assorted algae pollution, with beating crusher frond is smashed again, by the aperture is the silk cover filtering of 120-130 μ m, and making filtrate is the bolting silk of 90-100 μ m by the aperture, and the algae section that obtains is cultivated in 10-15 ℃ of incubator, light intensity is 2000-3000lux, light application time is 10-14 hour, and through 3-7 days, the algae section just can grow the rhizoid and the innovation of sufficient amount and length.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101130939A CN100566556C (en) | 2007-11-04 | 2007-11-04 | The method of polysiphonia urceolata sporophyte direct seedling formation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007101130939A CN100566556C (en) | 2007-11-04 | 2007-11-04 | The method of polysiphonia urceolata sporophyte direct seedling formation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101147463A true CN101147463A (en) | 2008-03-26 |
| CN100566556C CN100566556C (en) | 2009-12-09 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007101130939A Expired - Fee Related CN100566556C (en) | 2007-11-04 | 2007-11-04 | The method of polysiphonia urceolata sporophyte direct seedling formation |
Country Status (1)
| Country | Link |
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| CN (1) | CN100566556C (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392584A (en) * | 2013-06-08 | 2013-11-20 | 中国海洋大学 | Seedling breeding method using asparagus spores |
| CN110036902A (en) * | 2019-04-24 | 2019-07-23 | 大连海事大学 | Marine Red Alga Polysiphonia Urceolata filiform clone method for culturing seedlings |
-
2007
- 2007-11-04 CN CNB2007101130939A patent/CN100566556C/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103392584A (en) * | 2013-06-08 | 2013-11-20 | 中国海洋大学 | Seedling breeding method using asparagus spores |
| CN103392584B (en) * | 2013-06-08 | 2015-03-11 | 中国海洋大学 | Seedling breeding method using asparagus spores |
| CN110036902A (en) * | 2019-04-24 | 2019-07-23 | 大连海事大学 | Marine Red Alga Polysiphonia Urceolata filiform clone method for culturing seedlings |
| CN110036902B (en) * | 2019-04-24 | 2021-12-24 | 大连海事大学 | Polysiphonia urceolata filamentous clone seedling culture method |
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| Publication number | Publication date |
|---|---|
| CN100566556C (en) | 2009-12-09 |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091209 Termination date: 20101104 |