CN101130753A

CN101130753A - Indigenous microorganism nutrient solution

Info

Publication number: CN101130753A
Application number: CNA2006100304407A
Authority: CN
Inventors: 翁天波
Original assignee: Shanghai CNPC Enterprise Group Co Ltd
Current assignee: Shanghai CNPC Enterprise Group Co Ltd
Priority date: 2006-08-25
Filing date: 2006-08-25
Publication date: 2008-02-27

Abstract

The invention discloses a microbe nourishing liquor to extract oil, which comprises the following parts: 1-3% aquacare, 0. 01-0. 05% MgSO4.7H2O, 0. 05-0. 1% FeSO4.5H2O, 0. 05-0. 1% NH. Cl, 0. 5-1% amino acid, 0. 05-0. 25%NH.NO2, 0. 1-0. 5% Na2HPO3, 0. 05-0. 1 % CaCl2 , 0. 05-0. 1% ZnCl2, 1-4% molasses, 0. 1-0. 5% rhamnose, 1-5% alkyl glycoside and residual water.

Description

A kind of indigenous microorganism nutrient solution

Technical field

The present invention relates to a kind of nutritive medium of oil extraction microbial conenoses.

Background technology

Nutrition problem for microorganism relates to two aspects: one is microorganism is cultivated corresponding substratum before injecting oil well nutritive ingredient, and another is the nutritive substance of required interpolation in well.Though the screening of substratum bacterium in mining site is used is the nutritive substance survival and reproduction with crude oil, but the bacterium liquid that is used for mining site is to cultivate meticulously at indoor special nutritive substance, these nutritive substances are commonly referred to substratum, mainly mix by organic substance and trace element, the needed substratum of different bacteriums is not necessarily identical, with a kind of bacterium, it function that possesses also may be different, even can differ greatly with different culture medium culturing.Make a kind of substratum can either satisfy the requirement of multiple microbial reproduction simultaneously, can make their breedings in the short time reach maximum density and have the strongest activity again, and making them keep needed functions of use fully, is a research work that difficulty is very big.

Microbial strains at a certain amount of nutrition of underground needs to keep its growth, breeding and metabolism.Nutrition mainly is carbon source, nitrogenous source, phosphorus source, and wherein carbon source is an in-place oil, need not supply; Other nutritive ingredients need to add.By dissimilar nutrition agent screening experiments, select by ammonium salt, phosphoric acid salt and somatomedin etc. and form, and inject the nutrient solution of sewage preparation with mining site.Because local water or injection water contain the required trace element of microorganism growth, do not need to replenish.So stratum condition has determined the nutritive substance of required interpolation.Compatibility, the former wet goods experimental result of dispersion show that selected nutritive medium and local water (or sewage) compatibleness is good, and the energy for growth of bacterium is also better with the emulsifying property of oil.

Should under formation condition, have thermostability and chemical stability to the required nutritive substance of microorganism that injects the stratum, and can not react with the inorganic salt in the liquid of stratum and precipitate, with the antifouling stratum.In addition, in containing the stratum of clay, nutritive medium should be unlikely to cause stratum clay swelling and fine migration.For avoiding that these problems take place, guarantee the engineering success, should utilize stratum water sample and rock sample to carry out the laboratory experiment of relevant this respect.

Summary of the invention

The present invention is directed to above-mentioned the deficiencies in the prior art, propose a kind of indigenous microorganism nutrient solution and activate indigenous microorganism in the oil reservoir, thereby reach the purpose that improves the microbe oil production effect.

Indigenous microorganism is meant that inhabiting for a long time in the oil reservoir with the hydrocarbon is the microorganism of sole carbon source growth.The indigenous microorganism oil recovery technique is a kind of biotechnology that increases oil recovery by the biological activity of adjusting intrinsic group in the oil reservoir.Indigenous microorganism is a metastable microflora on the inherent value volume and range of product of certain period in the stratum.This is to enter oil reservoir with the injection water that contains a large amount of bacteriums in oilfield development process.Because the difference of bacterial species etc., the incompatibility ground environment that has and dying, what have passs on into dormant state, also has a small amount of bacterium to adapt to abominable ground environment, and utilizes nutrition limited in the stratum to grow slowly and metabolism.Because the mesotrophic shortage in stratum has hindered a large amount of breedings of indigenous microorganism, therefore, as long as an amount of nutritive substance is provided and is injected into oil reservoir and just can selectively will help improving the bacterium activation of recovery ratio by appropriate means, and tachymetabolism is of value to the product of crude oil flow, raising reservoir development degree and recovery ratio.

The technical solution adopted in the present invention is:

A kind of indigenous microorganism nutrient solution comprises following component by weight percentage:

Urea 1%～3%, MgSO ₄7H ₂O 0.01%～0.05%, FeSO ₄5H ₂O 0.05%～0.1%, NH.Cl 0.05～0.1%, amino acid 0.5～1%, NHNO ₂0.05 Na～0.25%, ₂HPO ₃0.1%～0.5%, CaCl ₂0.05%～0.1%, ZnCl ₂0.05%～0.1%, molasses 1%～4%, rhamnosyl 0.1～0.5%, alkyl glycoside 1%～5%, surplus is a water.

According to one or more the mixture that also can add in different these nutritive mediums of ground environment and temperature range in the following component: Amino Trimethylene Phosphonic Acid 0～0.5%, butter cream 0～0.5%, sheep blood 0～1% and MgCl24HzO 0～0.02%.

Above-mentioned nutritive medium using method is as follows: by injection pump biological nutrient liquid and pressurized air are injected the stratum by the waterflood-transmission line bypass, every mouthful of annual construction 4～6 of well is taken turns, and the interval is 50～70 days between the two-wheeled, construction is 2 years continuously, every individual well of taking turns is divided 3 injections, annotates closing well 1 day 1～2 day at every turn.

Beneficial effect of the present invention: indigenous microorganism is to rely on sole carbon source " hydro carbons " in the oil reservoir for nutrition, and activating indigenous microorganism is exactly to provide enough nutritive substances to indigenous microorganism, makes its breeding, growth.By using this nutritive medium to have economic attractiveness to marginal production fields, cost is low, and effective period is long, and required equipment is simple.Do not need to inject microorganism to oil reservoir.Therefore producing microbial or required program and the expense of metabolite increase do not need to make a living.Adopt traditional water filling uphole equipment can reach construction requirement.Microbial nutrient solution is with low cost and not influenced by crude oil price; Can be used for various types of crude oil; Do not damage the stratum, can in same oil well, repeatedly use; Do not pollute stratum and ground environment; Improve recovery ratio 10% than original method.

Embodiment

The present invention is further illustrated below in conjunction with embodiment:

Embodiment 1

With 35000 kilograms of daily water-injection rates is example, with 760.79 kilograms in urea, 6.63 kilograms of NH4CL2,304.31 kilograms in amino acid, 190.20 kilograms of 57.06 kilograms of NH3NO, Na2HPO3,30.43 kilograms of 7.61 kilograms of MgSO47H2O, FeSO45H2O, CaCl ₂38.04 kilogram, 26.63 kilograms of ZnCL2,760.79 kilograms in molasses, 76.08 kilograms of rhamnosyls, after 760.79 kilograms of alkyl glycoside are mixed in material-compound tank, by injection pump biological nutrient liquid and pressurized air are carried out guide's experiment by waterflood-transmission line bypass injection stratum, every mouthful of each construction 5 of well is taken turns, construction is 2 years continuously, every individual well of taking turns is divided 3 injections, annotates closing well 1 day 1 day at every turn.

This method improves recovery ratio 10% than original method, and water ratio descends 2%.

Embodiment 2

With 35000 kilograms of daily water-injection rates is example, with 384.74 kilograms in urea, the NHCL19.24 kilogram, 384.74 kilograms in amino acid, 19.24 kilograms of NH3NO, 338.47 kilograms of Na2HPO, 19.24 kilograms of MgSO47H2O, 38.47 kilograms of FeSOa45H2O, 30.78 kilograms of CaCl2,38.47 kilograms of ZnCl2,384.74 kilograms in molasses, 192.37 kilograms of rhamnosyls, after 1923.71 kilograms of alkyl glycoside are mixed in material-compound tank, by injection pump with biological nutrition. liquid and pressurized air inject the stratum by the waterflood-transmission line bypass and carry out guide's experiment, every mouthful of each construction of well. wheel, construction is 2 years continuously, every individual well of taking turns is divided 3 injections, each notes 1 day, closing well 1 day.This method improves recovery ratio 10% than original method, and water ratio descends 2%.

Claims

1. an indigenous microorganism nutrient solution comprises following component: urea 1～3%, MgSO by weight percentage ₄7H ₂O 0.01～0.05%, FeSO ₄5H ₂O 0.05～0.1%, NH.Cl 0.05～0.1%, amino acid 0.5～1%, NHNO ₂0.05 Na～0.25%, ₂HPO ₃0.1 CaCl～0.5%, ₂0.05 ZnCl～0.1%, ₂0.05～0.1%, molasses 1～4%, rhamnosyl 0.1～0.5%, alkyl glycoside 1～5%, surplus is a water.