CN101126714A - Optical fiber biosensor application method - Google Patents
Optical fiber biosensor application method Download PDFInfo
- Publication number
- CN101126714A CN101126714A CN 200710058309 CN200710058309A CN101126714A CN 101126714 A CN101126714 A CN 101126714A CN 200710058309 CN200710058309 CN 200710058309 CN 200710058309 A CN200710058309 A CN 200710058309A CN 101126714 A CN101126714 A CN 101126714A
- Authority
- CN
- China
- Prior art keywords
- fiber
- optical fiber
- affinity membrane
- biomaterial
- interfere
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Landscapes
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The utility model discloses an application method of an optical fiber biosensor, comprising a sample cell, a section of non-core fiber positioned in the sample cell, an input fiber and an output fiber positioned on two ends of the non-core fiber. The testing process of the senor on the biologic body comprises the following steps: an affinity membrane combining the testing biologic material is applied on the surrounding surface of the non-core fiber, the interference produced by the certain wavelength of the sensor is maximized or infinitesimal; then the liquid with the testing biologic material is injected into a sample cell, the biologic material is combined with the affinity membrane, and with the increase of the thickness of the affinity membrane, the certain wavelength of the sensor corresponding to the maximized or infinitesimal interference is variable relatively, and the biological material information is attained by detecting the variation of wavelength corresponding to the maximized or infinitesimal interference. The utility model has the advantages of simple structure of the sensor, easy manufacturing, simple testing process, practicality and high sensitivity.
Description
Technical field
The present invention relates to a kind of application process of optical fiber biosensor, particularly a kind of application process based on the optical fiber biosensor of high-order mode interference in the optical fiber belongs to the optical fibre bio sensory field.
Background technology
Biology sensor is the important means that is used for the detection and Identification bioactive materials, and these active materials can be enzyme, protein, DNA, antibody, antigen, biological membrane etc.Biology sensor is being brought into play important role at the aspects such as exploitation, Food Inspection and toxic gas detection of the diagnosis of microorganism detection, drug screening, blood analysis, DNA analysis, antigen/antibody analysis, all kinds of diseases and detection, new drug.Existing biology sensor can be divided into microbiological sensor, immunosensor, tissus sensor, cell sensor, enzyme sensor, DNA sensor etc. by its function.In the biology sensor that constitutes by various different principle, optical biosensor, particularly optical fiber biosensor with its high sensitivity, can realize quick online detection and can be used for distinguishing feature such as on-the-spot detection and be subjected to general attention and developed rapidly.Adopt surface plasma body resonant vibration technology (Surface Plasmon Resonance, be called for short SPR) optical biosensor development with surprising rapidity especially since nineteen eighty-two Nylander etc. is used for the immunosensor field with the SPR technology first, and be used widely.SPR is at the wave of oscillation of the free electron of metal and dielectric interface propagation and the light wave fields generation resonance of propagating in dielectric with specific wavelength and polarization state, and makes the energy of light wave produce a kind of physical phenomenon of decay.When metal film and thickness, specific inductive capacity of being deposited on the material on the metal etc. changed, respective change can take place in SPR resonance wavelength or resonant angle.If bioactive materials is fixed on the metal film, when with another kind of bioactive materials generation binding specificity, because the variation of film thickness, SPR resonance wavelength or resonant angle can change.So, but the specificity combination between the different bioactive materials of employing SPR technology detection and Identification.At present, the SPR technology is widely used in the research of various sufacings, has become gordian technique and method in the bio-sensing, also becomes the important means of microorganism detection and research molecular reaction dynamics simultaneously.The method of sputter is generally adopted in the making of metal film among the SPR, requires equipment and other condition higher.The thickness of the required metal film of SPR is in tens nanometers, and needing accurately, control could obtain good SPR effect.Metal commonly used is materials such as gold, silver, and to Jin Eryan, its SPR resonance wavelength is at red spectral band, and will select the suitable polarization direction.
Summary of the invention
The object of the present invention is to provide a kind of application process of optical fiber biosensor, with this method detection of biological material have operating process simple, be easy to characteristics such as realization, sensitivity height.
The present invention is realized by following technical proposals, a kind of application process of optical fiber biosensor, described optical fiber biosensor comprises the sample cell 104 of sealing, one section coreless fiber 102 is set in the sample cell, and the two ends of coreless fiber are docked single mode input optical fibre 101 and single mode output optical fibre 107 without acceptance of persons.Detect the method for the biomaterial that comprises enzyme, cell, gene, antigen, antibody with above-mentioned optical fiber biosensor, it is characterized in that comprising following process:
1, at first coat the affinity membrane that can combine with detected biomaterial in coating of coreless fiber circumferential surface or dipping, so the single-mode optics that transmits by the single mode input optical fibre coreless fiber be fixed on the high-order modes that produce a plurality of circle symmetries in the affinity membrane of its circumferential surface and the multimode lightguide that surrounding medium constitutes, high-order mode is propagated along described multimode lightguide, produce multiple-mode interfence at the interface of coreless fiber and single mode output optical fibre, produce in certain wavelengths and interfere greatly or interfere minimum;
2, the liquid that will contain the biomaterial of needs detection then injects sample cell, then biomaterial combines with affinity membrane, affinity membrane thickness increases, so the propagation constant of the high-order mode of propagating in multimode lightguide is changed, interfere greatly or interfere the wavelength of minimum correspondence to produce corresponding the variation thereby make, measure to interfere greatly or interfere the variation of the optical wavelength of minimum correspondence can obtain the situation of coreless fiber surface biological affinity membrane generation combination, thus the detection and Identification biomaterial to be measured amount that whether exists and exists how much or concentration information.
The characteristics of optical fiber biosensor involved in the present invention are: 1) sensor construction is extremely simple, makes easily, only needs common single mode telecommunication optical fiber and coreless fiber, and the optics that uses general optical fiber splicer can finish sensor is made.Because sensor itself is cheap, can realize disposable use truly.2) can adopt common chemical and other method that biomaterial is fixed on the coreless fiber surface, and need not special technique and equipment.3) optical wavelength detects and can adopt general spectroanalysis instrument to finish.4) have the Wavelength-encoding feature, and, be suitable for long-range and online detection because I/O optical fiber is single-mode fiber.
Description of drawings
Fig. 1 is the optical fiber biosensor structural representation.
Among the figure: 101 is the single mode input optical fibre; 102 is coreless fiber; 103 is the affinity membrane that is fixed on the biomaterial on coreless fiber surface; 104 is sample cell; 105 is the sample inlet; 106 is the optical fiber fixing glue; 107 is the single mode output optical fibre.
Fig. 2 is an optical fibre bio sensing experiment device instrument synoptic diagram.
Among the figure: 100 is optical fiber biosensor; 201 is wideband light source; 202 is Transmission Fibers; 203 is Water Tank with Temp.-controlled; 204 is wavelength demodulation device.
Fig. 3 is actual measurement antigen/antibody course of reaction curve map.
Embodiment
Below in conjunction with accompanying drawing the specific embodiment of the present invention is elaborated.As shown in Figure 1, single-mode fiber 101 adopts standard single-mode fiber (G652), and its core diameter is 8.2 μ m, and cladding diameter is 125 μ m, numerical aperture 0.14.At first its protective finish is removed, used the optical fiber cutting knife that its end face is cut into and its axis normal then.Coreless fiber 102 is pure silica fibre, and its diameter is 125 μ m.Use the optical fiber cutting knife that its end face is cut into and its axis normal, afterwards, the single-mode fiber 101 after the end face processing is put into optical fiber splicer with coreless fiber 102 two fused fiber splices are in the same place.The 55mm place uses the optical fiber cutting knife that it is cut off on coreless fiber, and itself and another section single-mould fiber 107 is welded together.
Fig. 2 is an optical fibre bio sensing experiment device instrument synoptic diagram.Wherein 100 is optical fiber biosensor.The output spectrum scope of wideband light source 201 is 1520-1565nm, and output power is 5mW; May in experiment, optical fiber biosensor 100 be placed Water Tank with Temp.-controlled 203 to the influence of measuring in order to eliminate temperature variation.The temperature of Water Tank with Temp.-controlled 203 is set in 25 ℃, and its temperature stability is ± 0.1 ℃.In the experiment, insert sample and inject conduit on sample inlet 105, optical fiber biosensor 100 is fixed on the support, and optical fiber biosensor 100 places Water Tank with Temp.-controlled 203 with support.204 is fibre optic spectral analyzer, as spectrum record and Wavelength demodulation equipment.
When the temperature stabilization of Water Tank with Temp.-controlled 203 after 25 ℃, inject conduit by sample and inject damping fluid, on the transmission spectrum of fibre optic spectral analyzer 204 records, determine a trough (interference minimal value) that produces by multiple-mode interfence, and measure the wavelength of this trough correspondence.The wavelength value of corresponding damping fluid is as the reference value of measuring.Afterwards, with the damping fluid emptying, inject the detected liquid that contains antigen.Because with the affine film of biologically active (antibody) 103 that is fixed on coreless fiber 102 surfaces specificity taking place, antigen combines, the propagation constant of the high-order mode of propagating in coreless fiber 102 is changed, and the trough that multiple-mode interfence is produced moves to the long wave direction, and its amount of movement is directly proportional with the binding capacity of antibody-antigen.By fibre optic spectral analyzer 204 record trough corresponding wavelength over time, can obtain the dynamic process and the binding capacity of antibody-antigen combination, thereby but whether have the information such as what or concentration that have the amount of the antigen of specificity binding characteristic and existence with the antibody that is fixed on coreless fiber 102 surfaces in the detection and Identification survey liquid to be measured.
Fig. 3 is actual measurement antigen/antibody reaction experiment result.Can find out along with the increase of time, constantly have antibody-antigen to take place thus, the thickness of the affinity membrane 103 on coreless fiber 102 surfaces is in continuous increase, thereby the trough (interference minimal value) that multiple-mode interfence produces in the coreless fiber 102 will move to the long wave direction.
By above embodiment as can be seen, the optical fiber biosensor that the present invention relates to is made very simple, need not the metal film of the nanometer grade thickness in the picture SPR technology, and employed optical fiber is conventional optical fiber, is easy to acquisition and cheap.The fixing of biomaterial can adopt any existent method to carry out.
The method that bioactive materials is fixed to the coreless fiber surface is multiple in addition, and as absorption method, sol-gel process, covalent bond is legal and LB mould technology etc., they are prior art.
Those skilled in the art are clear, and thought of the present invention can adopt the alternate manner beyond the above-named embodiment to realize.
Claims (1)
1. the application process of an optical fiber biosensor, described optical fiber biosensor comprises the sample cell (104) of sealing, one section coreless fiber (102) is set in the sample cell, the two ends of coreless fiber are docked single mode input optical fibre (101) and single mode output optical fibre (107) without acceptance of persons, detect the method for the biomaterial that comprises enzyme, cell, gene, antigen, antibody with above-mentioned optical fiber biosensor, it is characterized in that comprising following process:
1) at first coats the affinity membrane that can combine with detected biomaterial in coating of coreless fiber circumferential surface or dipping, so the single-mode optics that transmits by the single mode input optical fibre coreless fiber be fixed on the high-order modes that produce a plurality of circle symmetries in the affinity membrane of its circumferential surface and the multimode lightguide that surrounding medium constitutes, high-order mode is propagated along described multimode lightguide, produce multiple-mode interfence at the interface of coreless fiber and single mode output optical fibre, produce in certain wavelengths and interfere greatly or interfere minimum;
2) liquid that will contain the biomaterial of needs detection then injects sample cell, then biomaterial combines with affinity membrane, affinity membrane thickness increases, so the propagation constant of the high-order mode of propagating in multimode lightguide is changed, interfere greatly or interfere the wavelength of minimum correspondence to produce corresponding the variation thereby make, measure to interfere greatly or interfere the variation of the optical wavelength of minimum correspondence can obtain the situation of coreless fiber surface biological affinity membrane generation combination, thus the detection and Identification biomaterial to be measured amount that whether exists and exists how much or concentration information.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710058309 CN100565188C (en) | 2007-07-19 | 2007-07-19 | The application process of optical fiber biosensor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200710058309 CN100565188C (en) | 2007-07-19 | 2007-07-19 | The application process of optical fiber biosensor |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101126714A true CN101126714A (en) | 2008-02-20 |
| CN100565188C CN100565188C (en) | 2009-12-02 |
Family
ID=39094793
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200710058309 Expired - Fee Related CN100565188C (en) | 2007-07-19 | 2007-07-19 | The application process of optical fiber biosensor |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100565188C (en) |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101246119B (en) * | 2007-03-23 | 2011-06-08 | 国家纳米技术与工程研究院 | Optical fiber biological sensing system |
| CN102439425A (en) * | 2009-02-04 | 2012-05-02 | 奥斯坦德姆控股有限公司 | System for analysis of a fluid |
| CN103969221A (en) * | 2013-01-25 | 2014-08-06 | 中国计量学院 | Optical fiber refractive index sensor based on single mode-fine core-multimode-single mode structure |
| CN104251909A (en) * | 2014-09-18 | 2014-12-31 | 贵州大学 | Biosensor structure and preparation method thereof |
| CN105277489A (en) * | 2014-07-23 | 2016-01-27 | 英飞凌科技股份有限公司 | Sensing systems and methods using a coupling structure |
| CN106596452A (en) * | 2016-12-30 | 2017-04-26 | 重庆理工大学 | Hydrogen sulfide gas sensor and manufacturing method thereof and hydrogen sulfide concentration detection method |
| CN108759883A (en) * | 2018-05-21 | 2018-11-06 | 杭州光飞秒科技有限公司 | Mach-Zehnder interferometer in the optical fiber cable of straight waveguide is inscribed based on femtosecond laser |
| CN108917857A (en) * | 2018-09-13 | 2018-11-30 | 东北大学 | A kind of long-distance surface plasmon resonance sensor based on coreless fiber |
| CN109991193A (en) * | 2019-04-22 | 2019-07-09 | 重庆理工大学 | The production method of centreless multimode fibre these hydrogen sulfide gas sensor and its detection method of sensor and sulfureted hydrogen gas concentration |
| CN110308115A (en) * | 2019-06-14 | 2019-10-08 | 深圳大学 | A kind of interference-type optical fiber spr sensor |
-
2007
- 2007-07-19 CN CN 200710058309 patent/CN100565188C/en not_active Expired - Fee Related
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101246119B (en) * | 2007-03-23 | 2011-06-08 | 国家纳米技术与工程研究院 | Optical fiber biological sensing system |
| CN102439425A (en) * | 2009-02-04 | 2012-05-02 | 奥斯坦德姆控股有限公司 | System for analysis of a fluid |
| CN103969221A (en) * | 2013-01-25 | 2014-08-06 | 中国计量学院 | Optical fiber refractive index sensor based on single mode-fine core-multimode-single mode structure |
| US10345227B2 (en) | 2014-07-23 | 2019-07-09 | Infineon Technologies Ag | Sensing systems and methods using a coupling structure |
| CN105277489A (en) * | 2014-07-23 | 2016-01-27 | 英飞凌科技股份有限公司 | Sensing systems and methods using a coupling structure |
| CN105277489B (en) * | 2014-07-23 | 2019-11-05 | 英飞凌科技股份有限公司 | Use the sensing system and method for coupled structure |
| CN104251909A (en) * | 2014-09-18 | 2014-12-31 | 贵州大学 | Biosensor structure and preparation method thereof |
| CN104251909B (en) * | 2014-09-18 | 2016-05-11 | 贵州大学 | A kind of biosensor structure and preparation method thereof |
| CN106596452A (en) * | 2016-12-30 | 2017-04-26 | 重庆理工大学 | Hydrogen sulfide gas sensor and manufacturing method thereof and hydrogen sulfide concentration detection method |
| CN106596452B (en) * | 2016-12-30 | 2019-03-22 | 重庆理工大学 | The detection method of these hydrogen sulfide gas sensor and preparation method thereof and concentration of hydrogen sulfide |
| CN108759883A (en) * | 2018-05-21 | 2018-11-06 | 杭州光飞秒科技有限公司 | Mach-Zehnder interferometer in the optical fiber cable of straight waveguide is inscribed based on femtosecond laser |
| CN108917857A (en) * | 2018-09-13 | 2018-11-30 | 东北大学 | A kind of long-distance surface plasmon resonance sensor based on coreless fiber |
| CN109991193A (en) * | 2019-04-22 | 2019-07-09 | 重庆理工大学 | The production method of centreless multimode fibre these hydrogen sulfide gas sensor and its detection method of sensor and sulfureted hydrogen gas concentration |
| CN110308115A (en) * | 2019-06-14 | 2019-10-08 | 深圳大学 | A kind of interference-type optical fiber spr sensor |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100565188C (en) | 2009-12-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100565188C (en) | The application process of optical fiber biosensor | |
| Ahmad et al. | Effect of taper geometries and launch angle on evanescent wave penetration depth in optical fibers | |
| Beres et al. | Tapered plastic optical fiber-based biosensor–Tests and application | |
| Anderson et al. | A fiber optic biosensor: combination tapered fibers designed for improved signal acquisition | |
| Lieberman | Recent progress in intrinsic fiber-optic chemical sensing II | |
| Zhao et al. | Mini review: Recent advances in long period fiber grating biological and chemical sensors | |
| CN108572141B (en) | Composite enhanced optical fiber biosensor and method for detecting concentration of biological protein molecules | |
| Barshilia et al. | Low-cost planar waveguide-based optofluidic sensor for real-time refractive index sensing | |
| Chen et al. | D-type optical fiber immunoglobulin G sensor based on surface plasmon resonance | |
| Sun et al. | Non-enzymatic glucose detection based on phenylboronic acid modified optical fibers | |
| Leung et al. | Label-free detection of DNA hybridization using gold-coated tapered fiber optic biosensors (TFOBS) in a flow cell at 1310 nm and 1550 nm | |
| Li et al. | Selective and sensitive Escherichia coli detection based on a T4 bacteriophage‐immobilized multimode microfiber | |
| Tong et al. | Surface plasmon resonance optical fiber sensor for refractive index detection without temperature crosstalk | |
| Dai et al. | State-of-the-art optical microfiber coupler sensors for physical and biochemical sensing applications | |
| CN100565190C (en) | Biochemical substances method for sensing and biology sensor optical sensing structure | |
| Wang et al. | Research and application of multi-channel SPR sensor cascaded with fiber U-shaped structure | |
| CN104359868A (en) | Tilted fiber bragg grating surface plasma resonance biosensor based on M-Z interference | |
| CN104913728B (en) | U-shaped biconical fiber Biosensor for Detecting Bio-layer and making and measuring method | |
| CN109342716B (en) | D-type optical fiber sensor based on single-mode-few-mode-single-mode structure | |
| CN107543814A (en) | A kind of biological sensing system based on 45 ° of dual drive symmetrical structure bullet light modulations | |
| CN107219192B (en) | It is a kind of based on the biomolecule of photonic crystal fiber in fine detection system | |
| CN210742122U (en) | Mark-free temperature compensation grating type micro-nano optical fiber biosensor | |
| CN100567957C (en) | A kind of many optical fiber surface plasmon resonance probes with temperature correction | |
| Jain et al. | Pervasive review of optic biosensors-based on surface plasmon resonance and its development | |
| CN111537471A (en) | Ammonia gas sensor and preparation method and use method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20091202 Termination date: 20100719 |