CN210742122U - Mark-free temperature compensation grating type micro-nano optical fiber biosensor - Google Patents
Mark-free temperature compensation grating type micro-nano optical fiber biosensor Download PDFInfo
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- CN210742122U CN210742122U CN201921457459.9U CN201921457459U CN210742122U CN 210742122 U CN210742122 U CN 210742122U CN 201921457459 U CN201921457459 U CN 201921457459U CN 210742122 U CN210742122 U CN 210742122U
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Abstract
The utility model discloses optic fibre biotechnology field, in particular to optic fibre biosensor field. A grating type micro-nano optical fiber biosensor free of marking temperature compensation comprises a large-diameter cylindrical optical fiber 1, a truncated cone-shaped optical fiber 2 and a small-diameter cylindrical optical fiber 3 which are sequentially connected, wherein a fiber Bragg grating 4 is arranged on the small-diameter cylindrical optical fiber 3, and the end of the large-diameter cylindrical optical fiber 1 is an output end. The utility model discloses small and exquisite and compact structure realizes temperature compensation when monitoring biological analyte (target DNA sequence) concentration, the utility model discloses need not the mark, simplified the program flow, solved operation professional strong, with high costs, the strong scheduling problem of background signal.
Description
Technical Field
The utility model discloses optic fibre biotechnology field, in particular to optic fibre biosensor field.
Background
With the continuous progress in recent years, the optical fiber sensing technology gradually permeates from the initial physical quantity measurement to the fields of life, chemistry, medical detection and the like, and has unique advantages such as flexible structure, low price, electromagnetic interference resistance, reusability, biocompatibility, high sensitivity, quick response and the like. As a promising optical fiber sensing device, the grating type micro-nano optical fiber sensor provides stronger evanescent waves to complete high-sensitivity detection, but generally, temperature cross sensitivity exists in biosensing measurement to influence the detection accuracy. Therefore, it is crucial to implement temperature compensation in the actual monitoring system at the same time.
DNA is one of the basic substances of life, which is widely present in all organisms. DNA carries genetic information and has the function of storing and transmitting information. The detection of specific DNA sequences is of far-reaching significance, so that the manufacture of a high-performance sensor is an unbearable task. The application of label-free fiber grating evanescent wave sensing technology to DNA biosensing is a research hotspot in the field of biomedical sensing at present. The grating type micro-nano optical fiber researched and developed by the method has the advantages of the micro-nano optical fiber, the wavelength coding, the intrinsic reflection characteristic, the convenience in preparation and the like of the grating, and can meet the requirements of a biological sensing technology.
Disclosure of Invention
The utility model discloses the technical problem that will solve is: how to overcome the defects of the prior art and construct a miniaturized optical fiber biosensor which has high sensitivity, high specificity, no mark, simplicity and convenience and can be detected in vivo.
The utility model adopts the technical proposal that: a grating type micro-nano optical fiber biosensor free of marking temperature compensation comprises a large-diameter cylindrical optical fiber 1, a truncated cone-shaped optical fiber 2 and a small-diameter cylindrical optical fiber 3 which are sequentially connected, wherein a fiber Bragg grating 4 is arranged on the small-diameter cylindrical optical fiber 3, and the end of the large-diameter cylindrical optical fiber 1 is an output end. As a preferred mode: the large diameter cylindrical optical fiber 1 is a glass optical fiber with a diameter of 125 μm, the small diameter cylindrical optical fiber 3 is a glass optical fiber with a diameter of 4 μm, and the truncated cone-shaped optical fiber 2 is a glass optical fiber with an end face diameter of 125 μm and an end face diameter of 4 μm.
As a preferred mode: the distance between the two end faces of the truncated cone-shaped optical fiber 2 is 3mm, the length of the fiber Bragg grating 4 is 3mm, and the period is 1070.49 nm.
The large evanescent field characteristic of the grating type micro-nano optical fiber device provides high-sensitivity sensing of the change of the external environment. When light is incident to the fiber grating, light waves transmitted in the optical fiber and meeting the Bragg condition are gradually accumulated after being reflected by the periodic grating, and then reflected waves are formed. The reflection wavelengths of the fundamental mode and the high-order mode of the grating type micro-nano optical fiber have different refractive index responses and the same temperature response. Temperature cross sensitivity is eliminated based on the drift of different mode wavelength differences of the micro-nano fiber bragg grating, and then biological analyte detection without temperature sensitivity is realized.
The utility model has the advantages that: the utility model discloses small and exquisite and compact structure realizes temperature compensation when monitoring biological analyte (target DNA sequence) concentration, the utility model discloses need not the mark, simplified the program flow, solved operation professional strong, with high costs, the strong scheduling problem of background signal. The response of the biomolecular interaction can be monitored in real time, the analyte can be rapidly evaluated, and the reagent can be regenerated and reused. The utility model has wide application prospect, for example, can be used for DNA sequence hybridization detection or early diagnosis and detection of other cancers such as lung cancer tumor markers and the like.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a reflection spectrum diagram of the fiber bragg grating of the present invention.
FIG. 3 is a graph showing the response of the real-time monitoring probe single-stranded DNA sequence and the target DNA sequence at a wavelength interval;
the optical fiber Bragg grating comprises 1 large-diameter cylindrical optical fiber, 2 circular truncated cone-shaped optical fiber, 3 small-diameter cylindrical optical fiber and 4 optical fiber Bragg grating.
Detailed Description
As shown in figure 1, one end of a glass fiber with the diameter of 125 microns is tapered to be manufactured into a large-diameter cylindrical fiber 1, a truncated cone-shaped fiber 2 and a small-diameter cylindrical fiber 3 which are sequentially connected, the diameter of the small-diameter cylindrical fiber 3 is 4 microns, the distance between two end faces of the truncated cone-shaped fiber 2 is 3mm, and a fiber Bragg grating 4 with the length of 3mm and the period of 1070.49nm is manufactured on the small-diameter cylindrical fiber 3 in a mode of combining a 193nm excimer laser with a phase mask plate.
The small-diameter cylindrical optical fiber 3 with the grating type micro-nano optical fiber 4 has a large evanescent field characteristic, the sensitivity of sensing the refractive index of the external environment is high, the sensitivity of the refractive index of a basic mode is 53.8nm/RIU, the sensitivity of the refractive index of a high-order mode is 304.5nm/RIU, and the sensitivity of the refractive index at a wavelength interval is about 210 nm/RIU; because the difference between the thermo-optic coefficient and the thermal expansion coefficient of the grating written on the same quartz fiber is not large, the temperature sensitivities of the reflection peaks of the fundamental mode and the high-order mode can be considered to be similar. Therefore, the wavelength difference of the reflection peaks of a plurality of modes of the grating type micro-nano optical fiber can remove the temperature cross sensitivity, realize the absolute refractive index measurement, and further detect the real-time response of the interaction of the biomolecules with high sensitivity.
In this example, the surface functionalization treatment was carried out by adsorption (polylysine). Because fiber surface itself has the negative charge, the utility model discloses can directly soak in the polylysine that shows positive charge, wherein polylysine is as the effect of bridge, connects probe single strand DNA sequence (concentration 20 mu M), and later the response that optical fiber sensing device can be used to monitor target DNA sequence (concentration 1 mu M) is received a little to the grating type. The reflection spectrum of the optical fiber sensor in this example, as shown in fig. 2, has reflection peaks of a fundamental mode and a higher-order mode. The invention reflects the interaction of the biomolecules by using the change of the wavelength difference value of the two reflection peaks. Fig. 3 shows wavelength interval response curves of a single-stranded DNA sequence of a real-time monitoring probe and a target DNA sequence of the grating type micro-nano optical fiber of the present invention. The utility model discloses small and exquisite and compact structure realizes temperature compensation when monitoring biological analyte (target DNA sequence) concentration, the utility model discloses need not the mark, simplified the program flow, solved operation professional strong, with high costs, the strong scheduling problem of background signal. The response of the biomolecular interaction can be monitored in real time, the analyte can be rapidly evaluated, and the reagent can be regenerated and reused. The utility model has wide application prospect, and can be used for DNA sequence hybridization detection or early diagnosis and detection of other cancers such as lung cancer tumor markers.
Claims (4)
1. A grating type micro-nano optical fiber biosensor free of marking temperature compensation is characterized in that: the fiber bragg grating optical fiber.
2. The label-free temperature-compensated grating type micro-nano optical fiber biosensor according to claim 1, wherein the grating type micro-nano optical fiber biosensor is characterized in that: the large-diameter cylindrical optical fiber (1) is a glass optical fiber with the diameter of 125 mu m, the small-diameter cylindrical optical fiber (3) is a glass optical fiber with the diameter of 4 mu m, and the truncated cone-shaped optical fiber (2) is a glass optical fiber with the diameter of 125 mu m at one end face and the diameter of 4 mu m at the other end face.
3. The grating type micro-nano optical fiber biosensor free of marking temperature compensation according to claim 2, wherein the grating type micro-nano optical fiber biosensor is characterized in that: the distance between two end faces of the truncated cone-shaped optical fiber (2) is 3mm, the length of the fiber Bragg grating (4) is 3mm, and the period is 1070.49 nm.
4. The label-free temperature-compensated grating type micro-nano optical fiber biosensor according to claim 3, wherein the grating type micro-nano optical fiber biosensor is characterized in that: the large-diameter cylindrical optical fiber (1), the round table-shaped optical fiber (2) and the small-diameter cylindrical optical fiber (3) are connected in an integrated manner.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201921457459.9U CN210742122U (en) | 2019-09-04 | 2019-09-04 | Mark-free temperature compensation grating type micro-nano optical fiber biosensor |
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| CN201921457459.9U CN210742122U (en) | 2019-09-04 | 2019-09-04 | Mark-free temperature compensation grating type micro-nano optical fiber biosensor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11846564B2 (en) | 2022-05-03 | 2023-12-19 | Western Digital Technologies, Inc. | Optical systems and methods for locating query symbols in a reference sequence |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11846564B2 (en) | 2022-05-03 | 2023-12-19 | Western Digital Technologies, Inc. | Optical systems and methods for locating query symbols in a reference sequence |
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