CN101113419A - Intensive production method for sulfur-free rhodospirillum - Google Patents
Intensive production method for sulfur-free rhodospirillum Download PDFInfo
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- CN101113419A CN101113419A CNA2007100149779A CN200710014977A CN101113419A CN 101113419 A CN101113419 A CN 101113419A CN A2007100149779 A CNA2007100149779 A CN A2007100149779A CN 200710014977 A CN200710014977 A CN 200710014977A CN 101113419 A CN101113419 A CN 101113419A
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- glass
- sulfur
- water tank
- rhodospirillum
- free
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Abstract
An intensive production method of sulfur-free rhodospirillum is characterized in that: sterilization purifying water is injected into a close glass gutter through a pipeline that is communicated with the glass gutter, culture mother liquor is added and pH is adjusted to 7.2-7.4, then sulfur-free rhodospirillum strains are added and the temperature is controlled at 26-30 DEG C, a glass water tank is communicated with the glass gutter by the pipeline, and the height of the glass water tank is adjusted, resulting in that the culture liquor is stirred punctually in the glass water tank and the glass gutter. The thickness of the sulfur-free rhodospirillum obtained from the bacterial solution of the invention is up to 100-150 million/ml, the thickness of protein in the bacterial cell is more than 60 percent, the thickness of water soluble B Group Vitamins is more than 0.4mg/g and the thickness of coenzyme Q10 is more than 2.5mg/g, meanwhile, the bacterial liquor obtained in the invention is pure with stable nutrient content. The bacterial liquor produced by the method can not only be used for environment protective breed aquatics and seedling cultivation, but also can be used as additive of health care foods for preventing diseases. Besides, the production cost is greatly reduced: thereby, the application scope can be enlarged.
Description
Technical field
The present invention relates to a kind of intensive production method of sulfur-free rhodospirillum.
Background technology
Sulfur-free rhodospirillum is that Athiorhodaceae (Rhodospirillaceae) can purify water, safeguard the water ecology balance, the bacterium body is rich in nutritive substance, it is the precious flora of environmental protection and aquaculture, its range of application enlarges day by day, and the U.S. adds material with it as protective foods, but this bacterium is cultivated and production also lags behind application, present open cultivation based on pond, groove, case, culture condition is difficult to control, easily pollutes, and not formation scale intensification is as yet especially at present produced.
Summary of the invention
The intensive production method that the purpose of this invention is to provide a kind of sulfur-free rhodospirillum, it can overcome the above-mentioned shortcoming of prior art, can satisfy the demand of broadened application day by day.
A kind of intensive production method of sulfur-free rhodospirillum, it is characterized in that in the closed glass tank, inject the sterilization water purification by the pipeline that is communicated with glass flume, add and cultivate mother liquor, adjusting pH is 7.2-7.4, adds the sulfur-free rhodospirillum bacterial classification again, and temperature control is at 26-30 ℃, glass water tank is communicated with glass flume by pipeline, and the height of regulating glass water tank is agitated nutrient solution on time in glass water tank and glass flume.
The present invention has changed tradition and existing open cultural method, adopt the glass flume of sealing, pass through pipe-line transportation, in aseptic, relative anaerobic, steady temperature (26-30 ℃), pH (7.2-7.4), comprehensive illumination (all around up and down,, 800-1000Lx) and nutrient solution stir regularly that wait can full-automatic control and running intensification production down, cultivation through 5-6d, sulfur-free rhodospirillum concentration reaches 10-15 hundred million/ml in the gained bacterium liquid, proteinic concentration expressed in percentage by weight＞60% in the thalline, concentration＞the 0.4mg/g of water-soluble (vitamin) B family, ubiquinone
10Concentration＞2.5mg/g, more existing production technique is done sth. in advance 1-2d, simultaneously gained bacterium liquid is pure, nutrient contg is stable.The bacterium liquid of producing with this law not only can be used for environment-protecting cultivation and grows seedlings, more can be used for disease preventing and treating protective foods add material, also greatly reduce production cost simultaneously, thereby can the broadened application scope.
Embodiment
Further specify the present invention below by embodiment.
In the closed glass tank of sterilization, inject water purification 100L by the pipeline that is communicated with tank through disinfection by ultraviolet light, add cultivation mother liquor 5L, NaCl 1kg, use Na
2CO
3It is 7.5-8.0 that the aqueous solution is regulated pH, add again through screening, reinforcement and the sulfur-free rhodospirillum bacterial classification 1000ml (1,500,000,000/ml) that amplifies through secondary, above-mentioned glass flume is placed big glass case or glass room, be equipped with 25 watts of osram lamps before and after up and down in the cupboard or in the room, temperature controller is housed in the cupboard, temperature is controlled at 26-30 ℃, close big glass case or room, osram lamp is both as light source, make thermal source again, be provided with glass water tank moving up and down in big glass case or the room, the latter is communicated with glass flume by pipeline, move down glass water tank and can draw nutrient solution in the glass flume, on move glass water tank and can again nutrient solution be moved into glass flume again, every 12h glass water tank moves up and down once, nutrient solution is agitated, detected bacterial concentration with 721 or 722 spectrophotometers on the 6th day, at the 620nm place, optical density value＞1.5 o'clock gained bacterium liquid can go out groove.
A plurality of glass flumes can be communicated with by pipeline it is combined, the size of each glass flume is (50-60) * (50-60) * (35-40) cm
3, its loam cake can be airtight, can open.
Described cultivation mother liquor is by NH
4Cl, KH
2PO
4, MgSO
4, sodium-acetate, yeast extract and water forms, their weight thousand marks are followed successively by 15-20 ‰, 8-12 ‰, 3-5 ‰, 35-40 ‰, 50 ‰, all the other are water.
Described cultivation mother liquor is in 121 ℃ of following heating 20min, and it is standby to be contained in the sterilizing bottle sealing.Described yeast extract also claims yeast extract, can buy from the market.
Described sulfur-free rhodospirillum belongs to probiotic bacterium, this bacterial classification is taken from the ocean, from photosynthetic bacteria (Photosynthetic Bacteria) Rhodospirillaceae, the strong vigor bacterial strain of obtaining through separation, screening and optimization in the rhodospirillum, it has the effect of broad-spectrum antiviral and enhancing immunity, this bacterial strain itself is nontoxic, can remove the organic waste in the water body, decomposing harmful substances, the weight percentage of bacterium protein reaches 59-67%, contained indispensable amino acid is complete, almost contains all water-soluble (vitamin) B families and (comprises vitamins B
11And B
12), vitamin K and ubiquinone
10Therefore it is purifying water body, antibiotic diseases prevention and the living organisms that abundant nutrition is provided, and is the optimal aperture bait that fish, shrimps, scallop and sea cucumber are grown seedlings, and also is to optimize the holocoenotic leading bacterial strain of aquaculture water.By the sulfur-free rhodospirillum that the present invention produces, (＞10 hundred million/ml) can make the shrimp shoot survival percent up to 90% to throw in 5-10kg bacterium liquid with every mu of shrimp pond.
Claims (3)
1. the intensive production method of a sulfur-free rhodospirillum, it is characterized in that in the closed glass tank, inject the sterilization water purification by the pipeline that is communicated with glass flume, add and cultivate mother liquor, adjusting pH is 7.2-7.4, adds the sulfur-free rhodospirillum bacterial classification again, and temperature control is at 26-30 ℃, glass water tank is communicated with glass flume by pipeline, and the height of regulating glass water tank is agitated nutrient solution on time in glass water tank and glass flume.
2. the intensive production method of sulfur-free rhodospirillum as claimed in claim 1 is characterized in that the size of described glass flume is (50-60) * (50-60) * (35-40) cm
3
3. the intensive production method of sulfur-free rhodospirillum as claimed in claim 1 is characterized in that described cultivation mother liquor is by NH
4Cl, KH
2PO
4, MgSO
4, sodium-acetate, yeast extract and water forms, their weight thousand marks are followed successively by 15-20 ‰, 8-12 ‰, 3-5 ‰, 35-40 ‰, 50 ‰, all the other are water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100149779A CN100552020C (en) | 2007-06-21 | 2007-06-21 | A kind of intensive production method of sulfur-free rhodospirillum |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100149779A CN100552020C (en) | 2007-06-21 | 2007-06-21 | A kind of intensive production method of sulfur-free rhodospirillum |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101113419A true CN101113419A (en) | 2008-01-30 |
| CN100552020C CN100552020C (en) | 2009-10-21 |
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ID=39021950
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100149779A Expired - Fee Related CN100552020C (en) | 2007-06-21 | 2007-06-21 | A kind of intensive production method of sulfur-free rhodospirillum |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450588A (en) * | 2014-12-24 | 2015-03-25 | 四川省星川生物科技有限公司 | Method for culturing rhodopseudomonas palustris |
| CN104529111A (en) * | 2014-12-29 | 2015-04-22 | 天津振邦水产养殖有限公司 | Method for removing organic pollutants in bottom mud of holothurian growing water area |
-
2007
- 2007-06-21 CN CNB2007100149779A patent/CN100552020C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104450588A (en) * | 2014-12-24 | 2015-03-25 | 四川省星川生物科技有限公司 | Method for culturing rhodopseudomonas palustris |
| CN104529111A (en) * | 2014-12-29 | 2015-04-22 | 天津振邦水产养殖有限公司 | Method for removing organic pollutants in bottom mud of holothurian growing water area |
| CN104529111B (en) * | 2014-12-29 | 2016-03-02 | 天津振邦水产养殖有限公司 | A kind of method removing sea cucumber growth waters Organic Pollutants in Sedimentary Mud |
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| Publication number | Publication date |
|---|---|
| CN100552020C (en) | 2009-10-21 |
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Granted publication date: 20091021 Termination date: 20120621 |