CN101100481B - Method for extracting fructus momordicae tetranuclear triterpene - Google Patents
Method for extracting fructus momordicae tetranuclear triterpene Download PDFInfo
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- CN101100481B CN101100481B CN2006100213501A CN200610021350A CN101100481B CN 101100481 B CN101100481 B CN 101100481B CN 2006100213501 A CN2006100213501 A CN 2006100213501A CN 200610021350 A CN200610021350 A CN 200610021350A CN 101100481 B CN101100481 B CN 101100481B
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- fruit
- fructus momordicae
- triterpene
- pectin
- filtration
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
A method for extracting fructus momordicae tetranuclear-triterpene is carried out by leaching flavine from fresh fructus momordicae skin by warm water, heating gelatinous protein in fruit cell, solidifying, crushing, leaching by 60-90 deg. C, de-bonding by pectase, filtering, concentrating and drying to obtain final product. Crude products containing tetranuclear-triterpene 43% 300-350g are extracted from fresh fruits 10000g. It's pure, safe, efficient, simple and cheap. It has no environmental pollution. The pumace can be made into feed.
Description
Technical field:
The present invention relates to from the method for fresh Fructus Momordicae extracting effective components, specifically a kind of extracting method of fructus momordicae tetranuclear triterpene.
Background technology:
Contain materials such as triterpene glucoside in the Lo Han Guo fruit with very high economic worth and pharmaceutical use, the method of extracting Momordica-Glycosides at present from Grosvenor Momordica is more: Chinese patent 87101850 disclosed extracting method are with fresh Fructus Momordicae fragmentation, making beating, warm water extraction, use synthetic resins DA201 acrylic type fractionation by adsorption again, 50% dissolve with ethanol, the decolouring of D-211 acrylic type negative resin, desalination, impurity elimination, distillation method reclaims ethanol, through the vacuum pump concentrating under reduced pressure, lyophilize makes; Chinese patent 200510071877.0 disclosed extracting method are: fresh Fructus Momordicae pulverizing → alcohol heating reflux extraction → macroporous resin separates, deionized water and ethanol are eluted to colourless respectively, be paste with the elutriant concentrating under reduced pressure again, and then separate with normal phase silicagel column, adding chloroform, methyl alcohol, water are eluent, reclaim the elutriant concentrating under reduced pressure, promptly obtain Momordica-Glycosides, these extraction processes: 1, complex process; 2, the rate of recovery is low; 3, unusual attached to the material rich in proteins thickness on the adsorption column, handle untimely, very easily corrupt smelly; 4, the pomace of extracting back remaining 95% becomes waste, contaminate environment, in addition, disclose 1994 the 2nd phases of Guangxi light industry that a kind of to extract triterpene from Grosvenor Momordica be the method for sweet taste glycoside, its process is to wash fruit, broken, add water counter-current lixiviate four times, boiled 30 minutes at every turn, cooling, add polygalacturonase (making hydrolyzed pectin), filter, sterilization, concentrate, this method is not owing to have to leach in advance the xanthein in the pericarp and the protein in the fruit cell is solidified, and make it stripping in fruit juice, the product mouthfeel is poor, the blue or green stink that has fresh Fructus Momordicae, and color is plain, and other sweet food product of application and development are restricted.
Summary of the invention:
The objective of the invention is to disclose the extracting method of the fructus momordicae tetranuclear triterpene that a kind of extraction process is simple, extraction yield is high, free from environmental pollution.
Technical scheme of the present invention is: with the fresh Fructus Momordicae fruit is raw material, and extracting method comprises the steps:
1. extract earlier the Grosvenor Momordica yellow pigment: with the Lo Han Guo fruit warm water cleaning, immerses in 60-65 ℃ the hot water, the yellow pigment in two hours leaching pericarps, leach liquor are after filtration, concentrated, spraying drying gets the Grosvenor Momordica yellow pigment;
2. the fruit after lixiviate goes out xanthein is placed warming apparatus, the temperature for the treatment of the Lo Han Guo fruit central part reaches after 105-110 ℃ the temperature 1-5 minute, makes the proteinaceous solid in the fruit cell change into colloid, can not stripping;
3. broken lixiviate: with the Lo Han Guo fruit fragmentation after the above-mentioned heating, weigh 2.5 times 60-90 ℃ hot water lixiviate with Grosvenor Momordica, insulation lixiviate four times, each 2 hours, leach liquor did not contain Grosvenor Momordica protein;
4. leach liquor takes off pectin: in vat liquor, add leach liquor weight 0.25% take off polygalacturonase, reflection 2 hours under 55-60 ℃ of condition, remove pectin;
5, take off the pectin after-filtration, concentrate, dry, promptly get the fructus momordicae tetranuclear triterpene crude product, the remaining pomace in filter back use centrifuge dewatering, through exposure in sunshine or drying room 80-90 ℃ of temperature dry, pulverizing, make poultry and livestock feed.
Advantage of the present invention is:
1, extracts yellow pigment earlier, can make the tetracyclic triterpene quality of extraction purer, and obtain a kind of safer food dye;
2, extraction process is simple, and cost is low; The present invention adopts heating method to make the intracellular proteinaceous solid of Lo Han Guo fruit turn to colloid, can not stripping, and broken again the extraction saved in the traditional method and will have been added a lot of solvents, resin absorption, complicated procedures of forming such as wash-out separation;
3, it is 43% crude product 3-3.5g that extraction yield height, every 100g fresh fruit can be carried tetracyclic triterpene content in fact;
4, than the more environmental protection of existing extraction process, the protein that the thick substances on traditional extraction process adsorption column top layer contains, very easily corrupt smelly, contaminate environment;
5, the pomace after the present invention extracts contains protein and the unsaturated fatty acids about 7%, can directly be processed into feed.
Embodiment:
Embodiment 1:
Get the heavy fresh Fructus Momordicae of 10000g, use warm water cleaning, immerse in 60 ℃ the hot water, leach the yellow pigment in the pericarp, leach liquor after filtration, concentrate, spraying drying, reclaim edible flavochrome, to insert in the thermostat container through the Grosvenor Momordica that leaches yellow pigment, one of them living Grosvenor Momordica is worn an aperture, insert the contact of temperature sensor, be placed on the center of 10000g Grosvenor Momordica, treat that fruit central part temperature reaches 105 ℃ of post-heating 5 minutes, make the proteinaceous solid in the Grosvenor Momordica cell change into colloid, can not stripping, the Lo Han Guo fruit fragmentation after will heating then, with 70 ℃ of hot water lixiviates, connect and soak four times, each 2 hours, the leach liquor of gained did not contain protein, adding the 250g polygalacturonase then reacts under 55-60 ℃ of condition and took off pectin in 2 hours, again after filtration, concentrate, drying must contain the crude product 300-350g of tetracyclic triterpene 43%, and the pomace after the extraction is processed into feed.
Embodiment 2:
The device autoclave of heating, other are with embodiment 1.
Embodiment 3:
Fresh Fructus Momordicae 1000g, the heating unit muffle furnace, other are with embodiment 1.
Claims (1)
1. the extracting method of a fructus momordicae tetranuclear triterpene is characterized in that: comprise the steps:
(1) in the warm water with 60-65 ℃ of clean fresh Fructus Momordicae fruit immersion, leach the yellow pigment in the pericarp, concentrate drying gets yellow food dye after filtration;
(2) will be the lixiviate fruit that goes out yellow pigment place warming apparatus to heat, treat that fruit central part temperature reached after 105～110 ℃ the temperature reheat 1～5 minute, make protein in the fruit in cell, be solidified into colloid can not stripping after, again with the fruit fragmentation;
(3) with the fruit fragmentation after the heating in the step (2), reusable heat water insulation lixiviate 4 times, each 2 hours;
(4) in step (3) gained vat liquor, add polygalacturonase, remove pectin;
(5) will remove behind the pectin vat liquor after filtration, concentrate, drying makes the fructus momordicae tetranuclear triterpene crude product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100213501A CN101100481B (en) | 2006-07-04 | 2006-07-04 | Method for extracting fructus momordicae tetranuclear triterpene |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006100213501A CN101100481B (en) | 2006-07-04 | 2006-07-04 | Method for extracting fructus momordicae tetranuclear triterpene |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101100481A CN101100481A (en) | 2008-01-09 |
| CN101100481B true CN101100481B (en) | 2010-11-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN2006100213501A Expired - Fee Related CN101100481B (en) | 2006-07-04 | 2006-07-04 | Method for extracting fructus momordicae tetranuclear triterpene |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960399A (en) * | 2012-11-07 | 2013-03-13 | 张雨生 | Application of momordica grosvenori sweet crystal in healthy sugar-free sweet moon cake making |
| CN102960519A (en) * | 2012-11-07 | 2013-03-13 | 张雨生 | Health-care sugar-free sweet buccal tablet and preparation method thereof |
| CN102960608A (en) * | 2012-11-07 | 2013-03-13 | 张雨生 | Health-care sugar-free sweetened sticky soft cake |
| CN103461952A (en) * | 2013-09-24 | 2013-12-25 | 张雨生 | Fresh momordica grosvenori aspartame containing non-damaged fresh momordica grosvenori health-care components and preparation method thereof |
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2006
- 2006-07-04 CN CN2006100213501A patent/CN101100481B/en not_active Expired - Fee Related
Non-Patent Citations (4)
| Title |
|---|
| 廖寄帆.鲜罗汉果汁提取新工艺.广西轻工业 2.1994,(2),第26-28页，特别是第27页右栏第22行至第28页左栏第13行. |
| 廖寄帆.鲜罗汉果汁提取新工艺.广西轻工业 2.1994,(2),第26-28页,特别是第27页右栏第22行至第28页左栏第13行. * |
| 鞠伟等.罗汉果研究综述.广西轻工业 4.2001,(4),第4-6页，第4页左栏第2段. |
| 鞠伟等.罗汉果研究综述.广西轻工业 4.2001,(4),第4-6页,第4页左栏第2段. * |
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| CN101100481A (en) | 2008-01-09 |
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