CN101096674A - bZIP transcription factor related to corn kernel growth, its coding gene and gene expression - Google Patents

Abstract

The invention relates to thirteen new genes of bzip transcription factor expressed in the shelled corn growth process and their sequence analysis and expression analysis. The name of bzip transcription factor gene is MSBZIP1-MSBZIP13, and the nucleic acid sequence is one of the below: (1)1-13 DNA sequence in the sequence table, (2)1-13 limited DNA sequence with more than 90% consanguinity in the sequence table and DNA sequence with the same coding functional protein. The protein which is coded by bzip transcription factor gene has 14-26 amino acid residue sequence in the sequence table or has the same active 14-16 derived protein sequence with 14-26 amino acid residue sequence by replacing, missing or adding 14-26 amino acid residue sequence. Thirteen new genes of bzip transcription factor in the core is reported firstly, which provides the therapy for analyzing the expression and control of the gene in the shelled corn growth process and utilizing these transcription factors.

Description

Grow relevant bZIP transcription factor, its encoding gene and genetic expression thereof with corn kernel

Technical field

The present invention relates to grow 13 relevant new bZIP transcription factor and encoding genes thereof, and the expression characteristic of these transcription factor genes in the seed growth course is provided with corn kernel.

Technical background

Corn (Zea mays L.) claims maize again, is the third-largest in the world food crop, and sown area is only second to paddy rice and wheat.From 2000, the ultimate production of corn and single rate all occupied first.Corn is not only the food crop of high yield, also is nutritious fodder crop and favorable industrial raw material crop.Corn kernel is the topmost production traits of corn.Except in fact also being important organ of multiplication, be the important models of Gramineae seed development research for people provide the grain source.The growth of corn kernel is from fertilization, and by the double fertilization process, sperm that goes out from the pollen tube tip release and ovum fusion form diplontic zygote, develop into embryo; After merging, another sperm and two polar cores develop into triploid endosperm.In whole growth course, seed under suitable temperature and moisture content condition, begins to sprout through body early embryo generation, maturation and dormancy then.

From molecular biological angle, the growth of seed and sprouting be one orderly, optionally genetic expression process, and transcription factor has played critical effect in this course.Transcription factor (transcription factor) is also referred to as trans-acting factor (trans-acting factor), is that a class can DNA be conjugated protein with eukaryotic gene promoter region cis-acting elements (cis-acting factor) specificity bonded.By between transcription factor and the cis-acting elements and the interaction between they and other associated protein, transcription factor can activate or suppress transcribing of downstream gene.According to the conservative property of transcription factor DNA in conjunction with the territory, typical plant transcription factor often comprises that bZIP structural domain, zinc refer to (Zinc-Finger) structural domain, MADS structural domain, MYC structural domain, MYB structural domain, Homeo structural domain, bHLH (alkaline helix-loop-helix) structural domain and AP2/EREBP structural domain etc.

Alkalescence leucine zipper (basic leucine zipper, bZIP) albumen is a maximum and the most conservative class transcription factor in the Eukaryotic transcription factor, and the common feature of such transcription factor is: (1) contains the basic domain that combines with specific DNA sequence; (2) the leucine zipper district of participation oligomerization effect closely links to each other with basic region; (3) the N-end of transcription factor contains the acid activatable district; (4) with dimeric form in conjunction with DNA, the basic region of peptide chain N-end combines with dna direct.In higher plant, bZIP albumen is relevant with the control that the storage of seeds genetic expression, photomorphogenesis and organ build up.

In corn kernel, less relatively about the research of bZIP class transcription factor.Gene as the most thorough a kind of bZIP of coding transcription factor of studying in the corn kernel, Opaque2 (O2) gene is not only regulated and control the important storage protein of corn, specific expressed as alpha-alcohol soluble protein also regulated corn b-32, the cyPPDK1 expression of gene simultaneously.In addition, other bZIP transcription factor also has a small amount of research in the corn, as the HBP-1b homology of OBF3.1 and OBF3.2 and wheat, expresses Histidine gene (Rhonda etc., 1993 that cell cycle regulation is relevant at the initial stage of seed germination; Tetsuya etc., 1991); Though very low, but still can discern the O2-box (Leslie etc., 2004) of 22kDa alpha-alcohol soluble protein promoter region with wheat EmBP-1 homologous mEmBP-1 expression amount in endosperm; In the seed dehydration, ABI can regulate the expression (Niu etc., 2002) of ABA response gene.These researchs show that all the bZIP transcription factor is expressed with regulation and control very important to higher plant gene.

Yet, in corn, particularly in the corn kernel, still have a large amount of bZIP transcription factors also not to be found and to further investigate.The discovery of new transcription factor and research will provide valuable help to genetic engineering modified corn kernel proterties.Along with the order-checking of model plant Arabidopis thaliana and rice genome is finished, people have transferred to the genome aspect from the individual gene aspect to the research of transcription factor.Studies show that of Riechmann (2000) has 6% gene approximately in the arabidopsis gene group be the encoding transcription factor, and the research of Duan (2005) is also shown in that the transcription factor of specifically expressing has 135 in the rice paddy seed.Be the gene expression regulation in the parsing corn kernel growth course, and the application of the genetically engineered of these transcription factors provide the foundation.

Summary of the invention

One of purpose of the present invention is to provide with corn kernel grows 13 relevant new bZIP class transcription factors, called after MSBZIP (Maize Seed bZIP) 1-13.

Two of purpose of the present invention is to provide the encoding gene of this transcription factor.

Three of purpose of the present invention is to provide the expression of this transcription factor in the corn kernel growth course.

For achieving the above object, the present invention studies thinking by genomics, adopted the transcription group analysis of full etap of corn kernel, the transcription factor of specifically expressing in the seed growth course has been excavated by system, 13 new bZIP transcription factor genes are provided altogether, and their expression characteristic has been analyzed.

The present invention adopts following technical scheme:

Grow relevant bZIP transcription factor MSBZIP1-13 with corn kernel, it is characterized in that this transcription factor is the protein with amino acid residue sequence of sequence 14-26 in the sequence table, or the amino acid residue sequence of sequence 14-26 is passed through replacement, disappearance or the interpolation of one or several amino-acid residue and has identical active by sequence 14-26 deutero-protein with the amino acid residue sequence of sequence 14-26.

Above-mentioned transcription factor is the protein with amino acid residue sequence of sequence 14-26 in the sequence table.

The encoding gene of the above-mentioned bZIP transcription factor MSBZIP1-13 relevant with the corn kernel growth is characterized in that this encoding gene has one of following nucleotide sequences:

1) dna sequence dna of sequence 1-13 in the sequence table;

2) with sequence table in the nucleotide sequence that limits of sequence 1-13 have the homology 90% or more and the identical function protein DNA sequence of encoding.

Above-mentioned encoding gene has the dna sequence dna of sequence 1-13 in the sequence table.

Above-mentioned and corn kernel are grown the expression of relevant bZIP transcription factor MSBZIP1-13, and its sequence signature is the continuous expression in the seed growth course of MSBZIP2, MSBZIP4, MSBZIP6, MSBZIP7, MSBZIP8, MSBZIP10, MSBZIP11, MSBZIP12 and MSBZIP13 in this transcription factor; MSBZIP1, MSBZIP3, MSBZIP5, MSBZIP9 are phasic specificity to express in growth course.

The invention provides 13 and be coded in the new gene of bZIP transcription factor of expressing in the corn kernel growth course, and they have been carried out further analysis, difference by contained conservative section can be divided into three major types with these 13 bZIP albumen, they all contain conservative alkaline leucine section, but on evolving, present two kinds of different patterns, referring to Fig. 2, Fig. 3.The sxemiquantitative pcr analysis, referring to Fig. 4, show: the gene of great majority coding bZIP transcription factor is a continuous expression in the seed growth course, only 4 (accounting for 1/3) that on expressing, present phasic specificity, infer that special expression interval may be consistent with the developmental process that corn kernel is grown, this lays a good foundation for the expression regulation network of further studying corn kernel from now on.

Description of drawings

Fig. 1 has shown that blastx and pfam that 13 new genes are done analyze.Wherein both parameters all are made as Evalue＜10 ^-10

Fig. 2 has shown the analysis to the conservative section of bZIP transcription factor.The conservative alkaline leucine zone of wherein red grid representative, the conservative unknown function section of yellow and green representative respectively.

Fig. 3 has shown the cluster analysis that utilization Megabase analysis software is done the bZIP transcription factor.Wherein O2 is as the root of cluster analysis.

Fig. 4 has shown the expression pattern analysis of bZIP transcription factor in the whole etap.Swimming lane 1-14: the RT-PCR that is 3,5,7,9,11,13,15,18,21,24,27,30,33,36 days the different bZIP transcription factors in pollination back respectively; CGAPDH is as confidential reference items.

Embodiment

Below in conjunction with specific embodiment, further set forth the present invention.Should be understood that these examples only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted concrete experiment condition in the following example, usually according to normal condition, as molecular cloning (Molecular Cloning:A Laboratory Manual, 3rd ed.) or molecular biology of plants one laboratory manual (Plant Molecular Biology-A Laboratory Manual, Melody S.Clark compiles, Springer-verlag Berlin Heidelberg, 1997) condition described in, or the condition of advising according to manufacturer.

Embodiment one: the acquisition of the new gene of bZIP transcription factor of expressing in the corn kernel growth course

1. the structure of corn kernel etap normolized cDNA library

In order to obtain the expressed sequence of whole kernel etap, collect back 3 days-36 days corn kernel of pollination, after their equal-volumes are mixed, extract total RNA (Trizol kit, Qiagen), with PolyATtract through Promega company ^RThe mRNA of mRNA Isolation System III Kit purifying gained is a template, utilizes the cDNA homogenization scheme based on the principle of reannealing kinetics, promptly by sex change one renaturation manipulation, the abundance of cDNA is reached unanimity, and finally sets up the cDNA library of homogenization.

2. the acquisition of coding bZIP transcription factor gene

Adopt order-checking universal primer T3/T7, dideoxy method (DYEnamic ET-Terminator CycleSequencing), dna sequence analysis instrument (MegaBACE 4500) checks order to 10,000 clones of random choose.Institute's calling sequence is analyzed through the PhredPhrap package, obtains the EST library of representing the seed etap to express.Institute's calling sequence is analyzed through blastx (http://www.ncbi.nlm.nih.gov/dbEST) and pfam (http://www.pfam.jouy.inra.fr), obtain the new gene of 13 coding bZIP transcription factors altogether, referring to Fig. 1, wherein except that 4 with paddy rice in the known function bZIP transcription factor homolog, all the other all are 9 new bZIP albumen that are Unknown Function, and we are with its called after MSBZIP (Maize Seed bZIP) 1-13.。

Embodiment two: the evaluation of bZIP transcription factor

Utilization MEME analysis software carries out the conserved regions piecewise analysis to these 13 bZIP transcription factors, referring to as Fig. 2, they all contain a conservative alkaline leucine zone, and MSBZIP7-12 also has the conservative section of a Unknown Function except containing the distinctive alkaline section of bZIP albumen.According to the number and the kind of contained conservative section, we are divided into 3 classes with these 13 transcription factors, and wherein MSBZIP1-6 belongs to the first kind; MSBZIP7-11 is classified as second class; The 3rd class then comprises MSBZIP12 and MSBZIP13.

Utilize Megabase3.0 software that these 13 bZIP transcription factors are carried out cluster analysis, referring to Fig. 3, the result shows that MSBZIP5-11 is more approaching on evolutionary distance, and MSBZIP1-4, MSBZIP12, MSBZIP13 and O2 are more approaching on distance, and this may disclose two kinds of evolution modellings that are present in these bZIP transcription factors.

Embodiment three: the expression pattern analysis of bZIP transcription factor

With 3-36 days the corn kernel RNA in back that pollinates is template, Oligo (dT) ₁₆Carry out reverse transcription as primer, obtain the cDNA of each time point of whole kernel etap.Sequence according to these 13 transcription factors designs primer respectively, referring to table 1, cDNA with each time point is that template is carried out sxemiquantitative PCR, the PCR product is through 1% agarose gel electrophoresis analysis, referring to Fig. 4, proof MSBZIP2, MSBZIP4, MSBZIP6, MSBZIP7, MSBZIP8, MSBZIP10, MSBZIP11, MSBZIP12 and MSBZIP13 are continuous expressions in the seed growth course, referring to Fig. 4-A; MSBZIP1, MSBZIP3, MSBZIP5, MSBZIP9 then are specific expressed in growth course, referring to Fig. 4-B.

The primer table of table 1.bZIP transcription factor

The gene title	Sequence
		MSBZIP1 MSBZIP2 MSBZIP3 MSBZIP4 MSBZIP5 MSBZIP6 MSBZIP7 MSBZIP8 MSBZIP9 MSBZIP10 MSBZIP11 MSBZIP12 MSBZIP13 cGAPDH	F：5′-CAGGCATTACAGTCAGAAGGG-3’ R：5′-CGGGGGTAGTAACAGATTAGAAC-3′ F：5′-AGAACGCAAGGGGTGCTCGA-3′ R：5′-GCCGGGGACATTCTCAAGCA-3′ F：5′-CTCTTTCACCGATACAACC-3′ R：5′-GCTTCACCACCTGAGTTT-3′ F：5′-CAAGTCCACAGGGTCATC-3′ R：5′-GAGCCAACTCCTCACATT-3′ F：5′-ACTAGGAAACAAAAACAACACAGCA-3′ R：5′-ATGAGAGAGAACTGAAGAGACAAAA-3′ F：5′-GGACCATGACTCCAAGTG-3′ R：5′-CGTATCCTCCTAAACCCA-3′ F：5′-TCTCCGTGAIAACCAACT-3′ R：5′-CTCTTCTCGGTGACTACAT-3′ F：5′-GATTCTTTTGGACTCTGCTGG-3′ R：5′-GCTGAGATGTTGCCTGGTAA-3′ F：5′-TTTTGATGAGCCTGTCGT-3’ R：5′-CGTGAACATTTGCTGGAC-3’ F：5′-CTGGGAGTGAACCAACAA-3′ R：5′-TCCCGAGTGAAIAACAGC-3′ F：5′-CCATGGTCCCGGCTTCTAAT-3′ R：5′-CCCAAGCCACAAGAAGCACC-3′ F：5′-CGAAGTCCAATAACGAGCAT-3 R：5′-AAGGAGGAAGCAGTTCAAGG-3 F：5′-GTTTATGGGCACCCTGTC-3′ R：5′-GATGCTCCAAACGACCTG-3′ F：5’-GTCACCGTCTTTGGCATCAG-3’ R：5’-CAACAACCGAGACATCCACA-3’

Sequence table

＜110〉Shanghai University

＜120〉grow relevant bZIP transcription factor, its encoding gene and genetic expression thereof with corn kernel

<160>26

<210>1

<211>795

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>1

gaattcggcc attacggccg ggggacaaca agcgtgctaa acagcaatat gctcagaggt 60

ctcgtgtccg gaagctgcag tatattgcag agcttgagag tagagtccag gcattacagt 120

cagaaggggt agaagtgtct gctgaaatgg agttccttag ccagcagaat atcatgctag 180

acctggaaaa caaagccttg aagcaaagag tggagagtct agcacaggaa caacttatta 240

agcgttttca acaggagatg tttgagcggg aaattggtcg tctcagaaca atatatcagc 300

aacagcagca acaacaggct cctgctcttg ttcgtagtaa cagcagggac cttgatgtgc 360

agttcgccaa cttgtctctg aaacacaaag accccaacac gggccgggat gctctctctg 420

ggcctcttcg tacttagttc tttccatcct tccgttcttg tgggttctat cagtggccac 480

cactgtgtct gtcatctcca ccaagaaagg agaagtgcac ttggattgtg tgaaaaacac 540

cggaaaaagg ctaatagcta tggatctgat gtttattatt tcgggatatg cacctggcgg 600

tcatcctcag ctcgcgtcca ttggtgatgc tcgaccagtc ggcggtgttt agggtttttg 660

tttttagcta gatgcttttc aagcatcatt gtaccatttt tcatgctagg gttctaatct 720

gttactaccc ccgttttcct gaactgtgtg cttcaagcat cttagtcatg tttggataaa 780

aaaaaaaaaa aaaaa 795

<210>2

<211>1454

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>2

cggcgcctna gaacagtgga tccccggcgc tgaggaattc ggccattacg gccggggggc 60

gccgactgcg caaccggcac caaccttcgc caggttttcg tgggctcttt caccgataca 120

accatcctcc agaacgcaag gggtgctcga ttgactctag ttacttagca gaaccaaaag 180

tggaaggcca tcaaagatac gaggctattt tctctccatg tctcggtcac cccacctccc 240

accccgctgc ccacctctgg gtcctcagat cactcgaagg gatgacggca tgttcacgca 300

aagctgcagg tttccatctg gggatacgtt cattggtgaa ccaccatgct ggctggatga 360

tctccttgaa gattctggga agctcccaag ctccctcctc tcagaagggc ctgcagcgat 420

tctgatgcca tcttagatgc gctgtcgaca ttccagagcc caatttatcc aatcgaggaa 480

tgtgatctaa actcaggtgg tgaagctgag gacttgttgg gtggagtcga aggtgatgaa 540

agtggttctg tggtcgaggc tggctgtttc tatggtccga attcgccaag acagaagagt 600

aggcttacaa cttcagagag ttccatggta aatgctgtgc ttgagaatgt ccccggcaac 660

cccttgcagt acctgaccat cgatgcctgc agtgatgtga atggtaatgt ggccaatgta 720

actgctgatg cctgtgatgc ttttggccat cctgatcaag acaagtcatt taaaaggcgc 780

tcagggcaaa gatctagggt ccgaaaactt caatacatcg ctgaacttga aagaactgtc 840

gaatcgcttc agaacattgg agctgatttg gccgtcagaa tgtcatcact tttccatctt 900

cacaatgctc tatcaatgga aaacaagcaa ctgaggatac atatttccag tcttcagcaa 960

gcaaaactga ttaaggatgg tcagacgcag gccctaaaga atgaagctga aaggttgaag 1020

cagatgtctg cccgtcaccg ccggagcaga agcgtcactt cctgttatga cctgagctca 1080

ttcggagcag atgctacggc agtcaattgg cagatgtctg acatggcaag gctcagcctg 1140

aatggaagct cctctgtccc caagaagcgg ctatggtata tgacagcagc cggccctaac 1200

ctccctcaga agataagtcg aggcattacc ctagataact tttcgctaac cttacccagg 1260

catatatttg agggcccatc tttgactagg atatcagttg gattcgctga attttgcaga 1320

ggctatcagc ttaggcccgg tttggttggg gttttaggct gtttttgctt tccaaaagcc 1380

aaaagatcaa ccaaaagact tttttcagaa gcagttgctt ttcgtatttt ttttaaacaa 1440

aaggaaaaaa aaaa 1454

<210>3

<211>1036

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>3

cattacggcc gggggaggca gttggtggta cgggaacgtc cgcacgccga acccagtcat 60

gtcgcaggcg gcgtccatat ccgctagccc cgggctaacc acctcagcca atcatgctct 120

tgaaagcgag tcagactccg acagcgaatc actgtatgag gtagagggag ttccatacga 180

gcgaggtaac agatccattg agacgaagcg aataagaagg atggtgtcca acagggagtc 240

tgcgcggcgg tctaggagga ggaaacaggc acagttgtct gatcttgagt cacaggttga 300

acgactcaaa ggtgaaaacg caacactgtt cctgcaactt tcagatgcca accaacagtt 360

cagtactgca gtcacagaca acagaatcct caaatccgat gtagaagcgt taagaattaa 420

ggtaaagatg gcagaggata tggtagcgag aagtgctgta tcgtgtggcc taggcgacct 480

tggcctggca ccatacgtga actcaaggaa gatgtgccaa gctttgaatg tgctcacagg 540

gttggattta ctagggagtg atgcgttcag gggtccaacc gcaggtacac gagtacagaa 600

ctcaccagta cagagcactg caagcctaga gagtctggat aaccgaaagt ccaacgaggt 660

gaccagttgc gcggcggaca tttggccctg agcttcaagc cattgatctg attcaaagct 720

tgctccaccc tcaaaaaaag gaccagagtt tgtcaactca aatagctggt ggcttgagca 780

gagcttctgg cacaagttcg cttgaaccat atttccacat aagctttata ggatgcatca 840

ctcttttgat gtcacttagt ttcaagtata aaatgaagct gtctttgtac aaagctgcaa 900

atgagaagta aatgaaacgt cctggacaaa cttgtgaagt aggaatgata cataggatct 960

atttccaggt cagatgagac agttatgttt acaacaaaaa gccatacatg ttgtttagga 1020

aaagaaaaaa aaaaaa 1036

<210>4

<211>1532

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>4

cattacggcc gggggacaat ggactagttg ctgcactgct tttgtgagtc accatgggaa 60

gcagtggagc agatacatcg tctaagccaa acaaggcatc cgccccacag gagcagcaac 120

aacctgctac ctcaggtgct gcaacaccgg ctgtttatcc agactggtcc agcttccagg 180

catatcctcc aatcccacca catgggttct tcccctcacc tgtggcatca agtccacagg 240

gtcatcctta catgtgggga gctcagccta tgattccacc atatggagca ccgccatatg 300

tcatgtaccc tcctggagtg tatgcacacc catctatggc ctcgggtgca catccattta 360

ctccttatgc cattacttct ccaaatggca atgctgatgc tactggaact actgctgtag 420

ctcgtgatac tgatggcaag ccctctgaag gcaaagataa aagtccaact aagagaccca 480

aaggaacttt gggcagcttg aacatgctta ctggaaagaa ccccagtgaa catggtaaga 540

cctcaggtgc atcagctaat ggagccactt ctcaaagtgg cgaaagtggc agtgacagtt 600

caagcgaagg gagtgaagga aattctcata atgattcata caagcatagt ggacaggaac 660

aagatggaga cgtacgaagt tcccagaatg gtgcatcacg ttctccatct gagggaaaat 720

ttaaccaagc tatggcaatc atgcccatgc catcaagtgg cccagtaact ggtccaacta 780

caaacctgaa tattgggatg gactattggg ccaacacagc aagctctgct cctgtaattc 840

atggcaaagt gaccccaaca acagttccag gggctgtggt cccagcagag caatggatac 900

aggatgaacg tgaactcaaa aggcaaaaaa gaaaacagtc taatagggag tctgcacgca 960

gatctaggtt gcgtaaacag gctgaatgtg aggagttggc tcaacgtgct gatgttttaa 1020

agcaggaaaa tgcttcactt agagatgaag taaaccgtat cagaaaagag tatgaggaac 1080

ttgtatcaaa gaataattca ctaaaggaaa aacttgaagg caaacaacac aaaactgatg 1140

aggcaggact taacaacaag ctgcaacatt ctggcaatga catccagaaa aaaggaaact 1200

aatcgagcta tcatgcaatg cttgcatagc atggccctaa ctagattatt gggaggcgca 1260

attgtagtat gcgtattaac tccttacttt attgcagtta attgttacca ggttgtgata 1320

atgttgatac caaccttgtg tgtgggtgtt tcctgtttca gatctatctt ggcttatctt 1380

ttagaagtca agatgtgact gaagatgtag gctgtgaccc agttcactgt atctgaaata 1440

gtggataagc tcaaatttac aatttgtatt gctgactaat attgttatgt atacctggca 1500

aaaggttttc tttgaaaaca aaaaaaaaaa aa 1532

<210>5

<211>906

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>5

aaatatggat gccttcaaca cgctgcagtt gctgtcaaga agctcaatca gcagctctac 60

aacagctgta ccattcgcta acccccacgg ttcgaacaag ggcctggggg ttctacatag 120

ttgttactac ttctgtagaa actagcacaa ctaggaaaca aaaacaacac agcacagcag 180

ccgagctgag ggctgaggca cctggaagaa gaatctgtag ccgtcgacca tgtcaaatcc 240

tatgatacaa ctttacccct cggacaaaaa tctaataccc tacatcaaca cctgctcagt 300

cgtcctatca gtatcaggtt accaccaaat tatatcagca gtcagaaagt caggagaagt 360

ttcagcctgt tacaaatttc cattcgattt ctcgtcctca agcgctttaa gcttctcatg 420

tagagatgta ttttctgagg tcaagccatc acacttctcc tgaagttgtt tcaattcttc 480

tttaagtgaa ctattttcct gctttagcaa atctgcacga ttggctactt cctcccattc 540

agtctgtttg cgcaatcttg agcgtcgagc tgactcccta tttgattgct tacgtttttg 600

tctcttcagt tctctctcat cctgtatagc cggatcactg agaacaatat cacgtgaatt 660

tgatgaagga gctgatattg gtgctgcaat aactttacca tgcatgggta cagaagcagt 720

accccagtaa tccactccca tattcatagt agttgctggg ccagccatgg gagagggaac 780

aggccaaaat ggcatcatag ggttaagcac aacaggccca gaagatggtt gtgtgggctc 840

aactgcagaa cttcgagtac cttactcccc ccggccgtna tggccgataa aaaaaaaaaa 900

aaaaaa 906

<210>6

<211>863

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>6

gccagaaatt gagaaaacat cttcccgtga agcgcctaaa gtcccacttc ctgatcagac 60

aagaaatgcc cctctcagaa agctgcctag ttctggcagt ataaatagtc tagaagaaag 120

ccatttcctt caggcttcgt tggatttatc tgacaatgct tcattagaga ggcgaatgtc 180

ttcagaaagt aatatgtcat attatctgag gaccatgact ccaagtgctt ttgagtcagc 240

acttcgtcaa aaggatggag agcttgcttc ttacatgtct cgcttggcct cattggaatc 300

tattcggaat tcactggcag aggagttggt gaaactgaca gaacaatgtg aaaagttacg 360

gactgaagcc gctgctgtgc ctggcttaag ggctgaattg gaagctctga aacaaaggca 420

cttccaagct ctagagttaa tgggggaacg tgatgaagag ttggaggagc taagaaatga 480

tattgttgac ctaaaagaga tgtacaggga gcaggtggat cttcttgtta gccggctcca 540

ggcattgggt gctcgtgtat agtgccggta tggatttgtc gtgccatcgg gcaaggcaag 600

acaggtctga gacttgagca tgttttcctt ttttattttg taaccgtaca acggcactga 660

aaatattcgt ttgtacacat caaaaacacc ttcggcgttg ggtttaggag gatacgttgt 720

tgtatactag atcaagtagt ttcacagttt tacgtgcatt cttttttatc tgtgttcatt 780

gaaatgtatc aaatttgaat ccatttgcat gtgtacctca gaggaatgca atcaatcaat 840

atatnggagc tctttaaaaa aaa 863

<210>7

<211>1257

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>7

cattacggcc ggggacaaaa gaaacacaat gctgccattg aggttcttga gaaaacgaaa 60

gctgcggtaa cccacttaca tactagatac atagttgata tgcaatcaat ggattcaact 120

gtgtcagaaa ttcagtatct ccgtgataac caactgtacc caagactatt ggatcttact 180

gaccggatgg caaaaatgtg ggaggacatg aagatacatc acgcaaatca gctgaaaatt 240

gtcctaaatc tgaagtctgt tgatattcct gattccaata ttgaaacaag tgattatcac 300

cacagccaca cacggcagct gcgtgatatt gtagataagt ggatcacaaa ttttactgac 360

ttcatgaact gtcaaaagga atacataaat gctctctatg gctggttgaa gttgaacctt 420

atacccatag agagcagcct gaaggagaaa gtagcatcgc cacctaggat gcagcaacct 480

cctatcaaag cctttcttta tgcctggcat gagcacctag ccaagctgcc tgatgatctt 540

gccaagacag ccattgtcac ttttcgagca gtcttggata ccatattggg tgttcaagat 600

gaggagttga aacagaagga gatctgtgag cagactcgta gggaatacat gcgtaaggcc 660

cgggcttttg aggattggtg ccacaagcat ccacagccca gagcgatcga cgtcgatcca 720

gatagcggtg aggggacagg acataaggat gtagtcaccg agaagagatt cgcagtggag 780

agcttgaaga gcaagcttga tgatgaaatt gaatcccata ataagctatc aaagcaggtc 840

agggagaagt ccctctcgat cctgaaggca catctcccag agctgttccg tgctctgacc 900

gacttctccg acgcctcctt cgaaatgtac tcgaagctga ggctgatgtc gttgatgcag 960

gatcagggaa acaactagat ggacagtatg ttcagaagtt aggatcagta atatccctcc 1020

cccaagtgtt gtaatgcatg tgttctaccc ctttctgtgg tatgtgtaaa aggctagtag 1080

ctcattggat gaggattctt gtttaattta ttggcttgat gaaaccatcg nggtacttgt 1140

agtgtggaca tatgctggat agttagcatg tgatgaactg attacaatct gtgggggtaa 1200

agccgtcgtc tatgtaaatg ataattttgt tgagatatca aaaaaagaaa aaaaaaa 1257

<210>8

<211>1625

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>8

cattacggcc ggggttaagg agattgacga gtacttcttg aaagcagcag caagtggtaa 60

tgacgttgtg attcttttgg actctgctgg tgggcggcct gatgccttag agatggaggc 120

aaacaaaggc gcaggaaaga aatccaaatc agcgaaggtt ttctccacgc tctcttggag 180

ctggtctttt aaatctcagc aggctaacag tgaatccaca gttctgaatt ctagtgcttc 240

tggatatggt taccatggca agacactaga gaagctttat gaagaggagc aaaaactcta 300

caagttaatc aaggatgaag aatttgcaag gctccagtat aagaaacatg tctcagtgct 360

acaaaagcta gaatcagggg agcatgatag actgcatgca gaaagagttc gggatgctat 420

tgaagaactg caaactcgga taatctcatt ggaggaagct gtgagtttag catgtttttc 480

catatcaaat ctcagagacg aggaattata tcctcagatt attgaattat ctgcagggct 540

tgtgcatatg tggaaaaaca tgcacgagtg ccatcaggtc cagaaccaca ttgcccagca 600

acttagtctc attggcaaca gacctgggag tgaaccaaca agtgacattc attaccaggc 660

aacatctcag ctggaaattg aggtgtctgg atggcacact gccttctgca atctcatcac 720

gttacagcgt gaatacatta gcatccttaa ccaatggatt agtctcacag actgccttcc 780

agatgatggt ggcttaatga ggagttcatc tggaatccgt agcctctcca aggaactcca 840

gcatgctctt gacaggctac cggagaaggt agcagctgaa gcaataaaga cctttatgtc 900

aatcatacac tctatagttg tacagcagag tgaagagcgc caactaaaga agaagtcaga 960

taatatggat agcaagttcc agacccagtt agagaaacac agtgataatt caattcaaaa 1020

ctcggcacag cctccaaata agaatcacta ttcagtatca aagaatgaaa cgaaactgga 1080

tgcatttaga aagaaggtcg aggaggagaa ggccagatac ctaacctctg ttagggaaag 1140

ccgagccatg acactgaaca atctgcagac agcccttcca aatgtgttcc atgctctgga 1200

gggattctca ggagtgtgcg tgcaggcatt tgaaggtatc agctgatgca gtgaagttgc 1260

tgttattcac tcgggagcgg cttcatctgc tatttgctcg tgacattgct acgatggctg 1320

tgattattca ttgtaagtag gcgaagtata aactggttcc acgtgaatac ccttttcttc 1380

taattttcag aactttgaat agttttcgga ggattttatg aagtcaatat aggcataata 1440

gtcttgaata gatcacaatg tctcaactct caatttgaga agagatgtga agtatcataa 1500

aacatatgtg gctttaaatt atggaaagga ttcatgcaga cttcaactgc acgttctcat 1560

ggtatgatga tgctagtcat ctcaaaaaaa ttccaatgta tttgttcaga aaaaaaaggg 1620

aaaaa 1625

<210>9

<211>1324

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>9

cattacggcc gggggacctg aacgataatt ttgagattga tgatagattt gaaanctcat 60

gctgcagttc tagatagaat gcttgcttgg gagaaaaaac tgtatgacga agtgaaggct 120

ggggagctta tgaaaaccga ttatcagaag aaggtgtctc tattgcagaa gcaaaagaaa 180

cggggtgtta aacttgaaac cttggagaag acaaaagctg ctgttagcca attgcacaca 240

aggtacattg tagacatgca atccatggat tcaacagttt cagaaataaa tcggcttcgg 300

gatagacagt tattcccaaa gttaatggac cttgttgatg gaatggccaa gatgtggagt 360

tctatgtacc gccatcatag aagtcagttc ctgatcattt ctggaataag aggtttcgag 420

attcctccta ttccaaggga gacaactgat tctcattaca tccagacttg tgagctacgt 480

gacattgtta gggagtggca tacgcagttt gagaaattga tggacaatca gaaagcatac 540

atcagagcac tcaatgcatg gctgaagctc aatctcatcc caattgagag caacataaag 600

gagaaggtct cctcccctcc aaggctggtg gatccaccca tcaagcacct gccgcgtgcc 660

tggcatgacg agctcgaaag gcttccgatt gagctcgcaa aaactgccat caaaaccttt 720

gctgaagtca taagcactat cgtgcatctt caggaagagg aagtaaacct aaggaggcgg 780

tgtgatgaaa ctcgtaggga tctgaaccga aagaaggcac agtttgagga ctggcatcaa 840

aagtacttgg agagacagac agcagaaact caaaacatag atcctgtcga ggataggaaa 900

cgcaccatcg aggaactaga gattcggctg agagaggagg aggggcacca tcttaggcat 960

gccaggcagg tgcgggagaa atcacttgca aacctccgga cacacctgcc agaacttttc 1020

aggaacatgt ccgattttgc ttacttttgc aaagacatgt acaacaatct tagaaaaact 1080

gctgcgctgt ccaaagatga agtctaaggc tgatgatatt tgtgcgtgtg tatgtatact 1140

cgacaagcag tagaatgttt gctggtaact tgtcctgatg ccgtctagaa tacataatag 1200

tcggcattaa ttatgtttgt aaattggaat ggacattccg gactccccat tgtttatttc 1260

catgctatgt actgcattgg tgcgtggata aaatctttga ttgctccaaa aaaaaaaaaa 1320

aaaa 1324

<210>10

<211>991

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>10

aaaaacaagc acgaggccat ccaggtccag aaccatatgc ccagcaagtt agtctccnng 60

gcaccagacc tgggagtgaa ccaacaagng acactcatcg ccagccaaca tctcagctgg 120

aaattgaggt gtctggatgg cacactgcct tctgcaatct tatcacatcg cagcgtgaat 180

acgttagcat ccttagccaa tggattggtc tcacagactg ccttccggat gatggtggct 240

taatgagaag ttcatctgga atccttagcc tctctgagga actccagcgt gctcttgaca 300

ggctaccaga gaaggtagca gctgaagcga taaagacctt tatgtcagtc atacactcta 360

tagttgtaca gcaaagtgaa gagcgccaac taaagaagaa gtcagataac atggatagca 420

agttccagac ccagttagag aaacacagtg aaaatacaat gcaaaactcg gcccagcctc 480

caaataagaa taactattca gcatcaaaga atggaacgaa actggatgca tttataaagc 540

aggtcgagga ggagaaagcc agatacctaa cctctgtgag ggtaagccga gccatgacac 600

tgaacagtct gcagacaagc cttcgaaatg tgttccatgc tctggaggga ttctcaggag 660

tgtgcgtaca ggcatttgaa ggtatcagca gatgcagtga agttgctgtt attcactcgg 720

gagcagcttc acctgctatt tcctcgtgac cttgcttcaa tgactgtgat tattcattgt 780

aagtaggcaa aggataaact ggttccacgt gaataccctt cgcttctaat tttcagaact 840

ttgaatagct ttcgggggat tatgtgaagt caatataggc ataatagtct tgaatacatc 900

acaatgtctc aacttacatt ttggttctta ttgcatggga tgtactgctc tgtacataat 960

catttacatt ttgaccgaac ctaaaaaaaa a 991

<210>11

<211>1355

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>11

ggaatcggcc attacggccg gggaaaaaga gacaacaaag caatttgttg tctcattctt 60

aggagctggc gagcactcgg catggcagat gctagttcga ggactgacac ctcgactgtt 120

gtagacatgg atgataagaa tcaaaggtta gaaaacggac agagtggagc catggtcccg 180

gcttctaatt catctgaccg gtctgacaga tctgacaaac ctatggacca gaaggtttta 240

cgacggtttg cccaaaatcg tgaggcagca agaaaaagcc ggctgaggaa aaaggcatat 300

gtgcaacagc tagagagcag taagctaaaa cttgcaagcc tggagcaaga actccagaaa 360

gctcgacagc agggaatctt catttctagt tctggagatc aaactcatac tatgagtgga 420

aatggagcta tgaccttcgg tttggaatat tctcgatggc aagaggaaca aaataagcag 480

ataaatgagc tgaggactgc agtaaatgct catgcaagtg atagtgacct ccgtcttata 540

gtcgatggga taatggcaca ttatgacgag atattcaggc tgaagggcat tgctgcaaag 600

gctgatgtgt tccatatact ttcaggcatg tggaaaacac ctgctgaaag gtgcttcttg 660

tggcttgggg gtttccgttc gtctgagctt ctaaagctcc ttgtgaatca gcttgaacct 720

ctaactgagc agcaattgat ggggctatcc aatctgcagc aatcttctca ncaggctgag 780

gatgcattgt cacaaggaat ggaagcattg caccagtcct tggcacaaac gctggctggg 840

tcccttggtc catcangatc ttcttggaac gtggcaaact atatgggttc aatggctatg 900

gccatgggaa aacttggcac cctccaaaat ttccttcccc aggctgacaa tctacgacag 960

cagaccttac atcagatgca acgtatcctg acaatccgac aagctgctcg tgcgcttctt 1020

gcaatccatg actacttttc acgtttgcgc gcactgagtt ctctttggct tgctaggcca 1080

cgagagtaac atcacccccg ttggggtcac ttgatgaact gatctgtgaa tgtgggactg 1140

ctggctgcaa actcgcaaag catttgcaga tgactggagg atcagtcctc tctgtacaag 1200

ttgtattaac atcttctggt ctcatattgt gctccagtgt tgtacaattt agggaaaagc 1260

ttggattaac ttgtaagtta tcatgaattt gttattggca tattgtacaa ttgtattagc 1320

aataaaaata gaagtacata agtgctggaa aaaaa 1355

<210>12

<211>978

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>12

cgggggtcaa aggaaagaaa agctcgatat ataacagaat tggagcggaa ggttcaaact 60

tttcagacag aggntantac tctttcagca cagctgacac tattccagag agacacaact 120

ggactttcag cagaaaatgc tgagctcaag ataaggttgc aggccatgga gcagcaggct 180

caactgcgtg acgctttaaa tgatgcactg aggcaagaag tggagaggct taagatagca 240

acgggcgaga tgtcgaagtc caataacgag catttcaata tgggaatgca gcacgtctcc 300

tacagccctt cattcttcca gctctcggag caacacacaa tgcagcagca tggaaacatt 360

cagctgccgc atcatttcca acagcctccg ctgaatgccc cgagccacca gatgctatcc 420

caccccaact ccctctcgaa catggtgcag caagaatcgc ttgggcggct ccaggggctg 480

gacattggga aaggatccat ggccgtgaag tcagaggctg aggtcgtggt gaagtcagag 540

ggcagctcta tatctgcggg tgaaagcaac accaccttct agctatacaa tacgtctgcc 600

ttctaagaag cctttgtttg gtttagagtt cttttgttca tagtctgaca tccttgaact 660

gcttcctcct tccctgtagt ttttttattc cattcgttac atttagttac cattgatcgg 720

ttgtgtcgat cgttgttgtc gtggtggctg attctatagg gacattggtg tgttggtttc 780

gagtttgtta tgtgtgtggt atggttggtt gtttcgagct agtgactgaa agggcaccga 840

gtgatttttt ggcagatttt cttttctcca cctgtttttt tgttatagaa caaccatgat 900

ttcttattct ggaaatgtgt acattgtgtt gctatgctaa tttgtagtaa tatttttgat 960

gaaaaaaaaa aaaaaaaa 978

<210>13

<211>942

<212>DNA

＜213〉corn kind Zea (Zea mays L.)

<400>13

ggcggcgctc agaacagtgg atccccgggc ctgaggaatt tggccattac ggccgggggt 60

acacaggcaa aagacttgag taataatatc aaaaagggtg gtaaggcaag cacaccattt 120

ggtattcagc aaagagctga gaagtccgca aggactaaac gatctacgaa gtcaatactg 180

cacgggctgt ctgatagtga taatagtgat tctgagttta tgggcaccct gtcgaggtca 240

ggatctcaca ccctgaagac aaaccgaaaa ttgtttcaaa atggcagtac tggtttgata 300

acacctcaat ccagtggact gatagaaaga gagagggtca gaactaaatc ccagccattg 360

gacccaaaag caacaagcaa cggagatcta catactatgg atgagtatca gtctgttatc 420

atgcagttaa gggatgaaaa ttcatcacta aaggaaagat tatcaaagat ggaagaatcg 480

atgtcccaag agctagtggt tgagcgtgac aagaataagt ctttgacaga gagggtggag 540

gatctgcaga gacaacttga gtctgcaacc aaggaacagg aggcattgat cgacatcttc 600

tcagaagaaa ggaaccgacg agaccaagaa gaggagagcc tgaggaggaa gctgaaggac 660

gcatcttcca ccatacagga tttgctggag caactgaatg ttgccaggaa aggccgcaaa 720

gtttgaggaa ataaactagt aacgttgctc cttgcgggca tcgtcttagt ttagagtttg 780

agggctagct gcatctacat tgtgctccag gtcgtttgga gcatcccatg tacagccaag 840

cagtagtgac cactgaagaa cacgttatgc tttatctgca gttagcagtt accctacaag 900

aaataacttg cgtagttatc tttttaagaa aaaaaaaaaa aa 942

<210>14

<211>217

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>14

Ile Arg Pro Leu Arg Pro Gly Asp Asn Lys Arg Ala Lys Gln Gln

5 10 15

Tyr Ala Gln Arg Ser Arg Val Arg Lys Leu Gln Tyr Ile Ala Glu

20 25 30

Leu Glu Ser Arg Val Glu Ala Leu Gln Ser Glu Gly Val Glu Val

35 40 45

Ser Ala Glu Met Glu Phe Leu Ser Gln Gln Asn Ile Met Leu Asp

50 55 60

Leu Glu Asn Lys Ala Leu Lys Gln Arg Val Glu Ser Leu Ala Gln

65 70 75

Glu Gln Leu Ile Lys Arg Phe Gln Gln Glu Met Phe Glu Arg Glu

80 85 90

Ile Gly Arg Leu Arg Thr Ile Tyr Gln Gln Gln Gln Gln Gln Gln

95 100 105

Ala Pro Ala Leu Val Arg Ser Asn Ser Arg Asp Leu Asp Val Gln

110 115 120

Phe Ala Gln Leu Ser Leu Lys His Lys Asp Pro Asn Thr Gly Arg

125 130 135

Asp Ala Leu Ser Gly Pro Leu Arg Thr Phe Phe Pro Ser Phe Arg

140 145 150

Ser Cys Gly Phe Tyr Gln Trp Pro Pro Leu Cys Leu Ser Ser Pro

155 160 165

Pro Arg Lys Glu Lys Cys Thr Trp Ile Val Lys Thr Pro Glu Lys

170 175 180

Gly Leu Trp Ile Cys Leu Leu Phe Arg Asp Met His Leu Ala Val

185 190 195

Ile Leu Ser Ser Arg Pro Leu Val Met Leu Asp Gln Ser Ala Val

200 205 210

Phe Arg Val Phe Val Phe Ser

215

<210>15

<211>420

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>15

Arg Arg Leu Arg Thr Val Asp Pro Arg Arg Gly Ile Arg Pro Leu

5 10 15

Arg Pro Gly Gly Ala Asp Cys Ala Thr Gly Thr Asn Leu Arg Gln

20 25 30

Val Phe Val Gly Ser Phe Thr Asn Thr Thr Ile Leu Gln Asn Ala

35 40 45

Arg Gly Ala Arg Leu Thr Leu Val Thr Gln Asn Gln Lys Trp Lys

50 55 60

Ala Ile Lye Asp Thr Arg Leu Phe Ser Leu His Val Ser Val Thr

65 70 75

Pro Pro Pro Thr Pro Leu Pro Thr Ser Gly Ser Ser Asp His Ser

80 85 90

Lys Gly Arg His Val His Ala Lys Leu Gln Val Ser Ile Trp Gly

95 100 105

Tyr Val His Trp Thr Thr Met Leu Ala Gly Ser Pro Arg Phe Trp

110 115 120

Glu Ala Pro Lys Leu Pro Pro Leu Arg Arg Ala Cys Ser Asp Ser

125 130 135

Asp Ser Ala Ile Leu Asp Ala Leu Ser Thr Phe Gln Ser Pro Ile Tyr

140 145 150

Pro Ile Glu Glu Cye Asp Leu Asn Ser Gly Gly Glu Ala Glu Asp

155 160 165

Leu Leu Gly Gly Val Glu Gly Asp Glu Ser Gly Ser Val Val Glu

170 175 180

Ala Gly Cys Phe Tyr Gly Pro Asn Ser Pro Arg Gln Lys Ser Arg

185 190 195

Leu Thr Thr Ser Glu Ser Ser Met Val Asn Ala Val Leu Glu Asn

200 205 210

Val Pro Gly Asn Pro Leu Gln Tyr Leu Thr Ile Asp Ala Cys Ser

215 220 225

Asp Val Asn Gly Asn Val Ala Asn Val Thr Ala Asp Ala Cys Asp

230 235 240

Ala Phe Gly His Pro Asp Gln Asp Lys Ser Phe Lys Arg Arg Ser

245 250 255

Gly Asp Arg Ser Arg Val Arg Lys Leu Gln Tyr Ile Ala Glu Leu

260 265 270

Glu Arg Thr Val Glu Ser Leu Gln Asn Ile Gly Ala Asp Leu Ala

275 280 285

Val Arg Met Ser Ser Leu Phe His Leu His Asn Ala Leu Ser Met

290 295 300

Glu Asn Lys Gln Leu Arg Ile His Ile Ser Ser Leu Gln Gln Ala

305 310 315

Lys Leu Ile Lys Asp Gly Gln Thr Gln Ala Leu Lys Asn Glu Ala

320 325 330

Glu Arg Leu Lys Gln Met Ser Ala Arg His Arg Arg Ser Arg Ser

335 340 345

Val Thr Ser Cys Tyr Asp Leu Ser Ser Phe Gly Ala Asp Ala Thr

350 355 360

Ala Val Asn Trp Gln Met Ser Asp Met Ala Arg Leu Ser Leu Asn

365 370 375

Gly Ser Ser Ser Val Pro Lys Lys Arg Leu Trp Tyr Met Thr Ala

380 385 390

Ala Gly Pro Asn Leu Pro Gln Lys Ile Ser Arg Gly Ile Thr Leu

395 400 405

Asp Asn Phe Ser Leu Thr Leu Pro Rro His Ile Phe Glu Gly Pro

4l0 415 420

<210>16

<211>210

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>16

Met Ser Gln Ala Ala Ser Ile Ser Ala Ser Pro Gly Leu Thr Thr

5 10 15

Ser Ala Asn His Ala Leu Glu Ser Glu Ser Asp Ser Asp Ser Glu

20 25 30

Ser Leu Tyr Glu Val Glu Gly Val Pro Tyr Glu Arg Gly Asn Arg

35 40 45

Ser Ile Glu Thr Lys Arg Ile Arg Arg Met Val Ser Asn Arg Glu

50 55 60

Ser Ala Arg Arg Ser Arg Arg Arg Lys Gln Ala Gln Leu Ser Asp

65 70 75

Leu Glu Ser Gln Val Glu Arg Leu Lys Gly Glu Asn Ala Thr Leu

80 85 90

Phe Leu Gln Leu Ser Asp Ala Asn Gln Gln Phe Ser Thr Ala Val

95 100 105

Thr Asp Asn Arg Ile Leu Lys Ser Asp Val Glu Ala Leu Arg Ile

110 115 120

Lys Val Lys Met Ala Glu Asp Met Val Ala Arg Ser Ala Val Ser

125 130 135

Cys Gly Leu Gly Asp Leu Gly Leu Ala Pro Tyr Val Asn Ser Arg

140 145 150

Lys Met Cys Gln Ala Leu Asn Val Leu Thr Gly Leu Asp Leu Leu

155 160 165

Gly Ser Asp Ala Phe Arg Gly Pro Thr Ala Gly Thr Arg Val Gln

170 175 180

Asn Ser Pro Val Gln Ser Thr Ala Ser Leu Glu Ser Leu Asp Asn

185 190 195

Arg Lys Ser Asn Glu Val Thr Ser Cys Ala Ala Asp Ile Trp Pro

200 205 210

<210>17

<211>382

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>17

Met Gly Ser Ser Gly Ala Asp Thr Ser Ser Lys Pro Asn Lys Ala

5 10 15

Ser Ala Pro Gln Glu Gln Gln Gln Pro Ala Thr Ser Gly Ala Ala

20 25 30

Thr Pro Ala Val Tyr Pro Asp Trp Ser Ser Phe Gln Ala Tyr Pro

35 40 45

Pro Ile Pro Pro His Gly Phe Phe Pro Ser Pro Val Ala Ser Ser

50 55 60

Pro Gln Gly His Pro Tyr Met Trp Gly Ala Gln Pro Met Ile Pro

65 70 75

Pro Tyr Gly Ala Pro Pro Tyr Val Met Tyr Pro Pro Gly Val Tyr

80 85 90

Ala His Pro Ser Met Ala Ser Gly Ala His Pro Phe Thr Pro Tyr

95 100 105

Ala Ile Thr Ser Pro Asn Gly Asn Ala Asp Ala Thr Gly Thr Thr

110 115 120

Ala Val Ala Arg Asp Thr Asp Gly Lys Pro Ser Glu Gly Lys Asp

125 130 135

Lys Ser Pro Thr Lys Arg Pro Lys Gly Thr Leu Gly Ser Leu Asn

140 145 150

Met Leu Thr Gly Lys Asn Pro Ser Glu His Gly Lys Thr Ser Gly

155 160 165

Ala Ser Ala Asn Gly Ala Thr Ser Gln Ser Gly Glu Ser Gly Ser

170 175 180

Asp Ser Ser Ser Glu Gly Ser Glu Gly Asp Ser His Asn Asp Ser

185 190 195

Tyr Lys His Ser Gly Gln Glu Gln Asp Gly Asp Val Arg Ser Ser

200 205 210

Gln Asn Gly Ala Ser Arg Ser Pro Ser Glu Gly Lys Phe Asn Gln

215 220 225

Ala Met Ala Ile Met Pro Met Pro Ser Ser Gly Pro Val Thr Gly

230 235 240

Pro Thr Thr Asn Leu Asn Ile Gly Met Asp Tyr Trp Ala Asp Thr

245 250 255

Ala Ser Ser Ala Pro Val Ile His Gly Lys Val Thr Pro Thr Thr

260 265 270

Val Pro Gly Ala Val Val Pro Ala Glu Gln Trp Ile Gln Asp Glu

275 280 285

Arg Glu Leu Lys Arg Gln Lys Arg Lys Gln Ser Asn Arg Glu Ser

290 295 300

Ala Arg Arg Ser Arg Leu Arg Lys Gln Ala Glu Cys Glu Glu Leu

305 310 315

Ala Gln Arg Ala Asp Val Leu Lys Gln Glu Asn Ala Ser Leu Arg

320 325 330

Asp Glu Val Asn Arg Ile Arg Lys Glu Tyr Glu Tyr Leu Val Ser

335 340 345

Lys Asn Asn Ser Leu Lys Glu Lys Leu Glu Gly Lys Gln His Lys

350 355 360

Thr Asp Glu Ala Gly Leu Asn Asn Lys Leu Gln His Ser Gly Asn

365 370 375

Asp Ile Gln Lys Lys Gly Asp

380

<210>18

<211>163

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>18

Gly Thr Arg Ser Ser Ala Val Glu Pro Thr Gln Pro Ser Ser Gly

5 10 15

Pro Val Val Leu Asn Pro Met Met Pro Phe Trp Pro Val Pro Ser

20 25 30

Pro Met Ala Gly Pro Ala Thr Thr Met Asn Met Gly Val Asn Tyr

35 40 45

Trp Gly Thr Ala Ser Val Pro Met His Gly Lys Val Ile Ala Ala

50 55 60

Pro Ile Ser Ala Pro Ser Ser Asn Ser Arg Asp Ile Val Leu Ser

65 70 75

Asp Pro Ala Ile Gln Asp Glu Arg Glu Leu Lys Arg Gln Lys Arg

80 85 90

Lys Gln Ser Asn Arg Glu Ser Ala Arg Arg Ser Arg Leu Arg Lys

95 100 105

Gln Thr Glu Trp Glu Glu Val Ala Asn Arg Ala Asp Leu Leu Lys

110 115 120

Gln Glu Asn Ser Ser Leu Lys Glu Glu Leu Lys Gln Leu Gln Glu

125 130 135

Lys Cys Asp Gly Leu Thr Ser Glu Asn Thr Ser Leu His Glu Lys

140 145 150

Leu Lys Ala Leu Glu Asp Glu Lys Ser Asn Gly Asn Leu

155 160

<210>19

<211>247

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>19

Ala Arg Asn Glu Asn Ile Phe Pro Ser Ala Ser Pro Thr Ser Ser

5 10 15

Asp Lys Lys Cys Pro Ser Gln Lys Ala Ala Phe Trp Gln Tyr Lys

20 25 30

Ser Arg Arg Lys Pro Phe Pro Ser Gly Phe Val Gly Phe Ile Gln

35 40 45

Cys Phe Ile Arg Glu Ala Asn Val Phe Arg Lys Tyr Val Ile Leu

50 55 60

Ser Glu Asp His Asp Ser Lys Cys Phe Val Ser Thr Ser Ser Lys

65 70 75

Gly Trp Arg Ala Cys Phe Leu His Val Ser Leu Gly Leu Ile Gly

80 85 90

Ile Tyr Ser Glu Phe Thr Gly Arg Gly Val Gly Glu Thr Asp Arg

95 100 105

Thr Met Lys Val Thr Asn Ser Arg Cys Cys Ala Trp Leu Lys Gly

110 115 120

Ile Gly Ser Ser Glu Thr Lys Ala Leu Pro Ser Ser Arg Val Asn

125 130 135

Gly Gly Thr Arg Val Gly Gly Ala Lys Lys Tyr Cys Pro Lys Arg

140 145 150

Asp Val Gln Gly Ala Gly Gly Ser Ser Cys Pro Ala Pro Gly Ile

155 160 165

Gly Cys Ser Cys Ile Val Pro Val Trp Ile Cys Arg Ala Ile Gly

170 175 180

Gln Gly Lys Thr Gly Leu Arg Leu Glu His Val Phe Leu Phe Tyr

185 190 195

Phe Val Thr Val Gln Arg His Lys Tyr Ser Phe Val His Ile Lys

200 205 210

Asn Thr Phe Gly Val Gly Phe Arg Arg Ile Arg Cys Cys Ile Leu

215 220 225

Asn Gln Val Val Ser Gln Phe Tyr Val His Ser Phe Leu Ser Val

230 235 240

Phe Ile Glu Met Tyr Gln Ile

245

<210>20

<211>292

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>20

Met Gln Ser Met Asn Ser Thr Val Ser Glu Ile Gln Tyr Leu Arg

5 10 15

Asp Asn Gln Leu Tyr Pro Arg Leu Leu Asp Leu Thr Asp Arg Met

20 25 30

Ala Lys Met Trp Glu Asp Met Lys Ile His His Ala Asn Gln Leu

35 40 45

Lys Ile Val Leu Asn Leu Lys Ser Val Asp Ile Pro Asp Ser Asn

50 55 60

Ile Glu Thr Ser Asp Tyr His His Ser His Thr Arg Gln Leu Arg

65 70 75

Asp Ile Val Asp Lys Trp Ile Thr Asn Phe Thr Asp Phe Met Asn

80 85 90

Cys Gln Lys Glu Tyr Ile Asn Ala Leu Tyr Gly Trp Leu Lys Leu

95 100 105

Asn Leu Ile Pro Ile Glu Ser Ser Leu Lys Glu Lys Val Ala Ser

110 115 120

Pro Pro Arg Met Gln Gln Pro Pro Ile Lys Ala Phe Leu Tyr Ala

125 130 135

Trp His Glu His Leu Ala Lys Leu Pro Asp Asp Leu Ala Lys Thr

140 145 150

Ala Ile Val Thr Phe Arg Ala Val Leu Asp Thr Ile Leu Gly Val

155 160 165

Gln Asp Glu Glu Leu Lys Gln Lys Glu Ile Cys Glu Gln Thr Arg

170 175 180

Arg Glu Tyr Met Arg Lys Ala Arg Ala Phe Glu Asp Trp Cys His

185 190 195

Lys His Pro Gln Pro Arg Ala Ile Asp Val Asp Pro Asp Ser Gly

200 205 210

Glu Gly Thr Gly His Lys Asp Val Val Thr Glu Lys Arg Phe Ala

215 220 225

Val Glu Ser Leu Lys Ser Lys Leu Asp Asp Glu Ile Glu Ser His

230 235 240

Asn Lys Lys Ser Lys Gln Val Arg Glu Lys Ser Leu Ser Ile Leu

245 250 255

Lys Ala His Leu Pro Glu Leu Phe Arg Ala Leu Thr Asp Phe Ser

260 265 270

Asp Ala Ser Phe Glu Met Tyr Ser Lys Leu Arg Leu Met Ser Leu

275 280 285

Met Gln Asp Gln Gly Asn Asn

290

<210>21

<211>392

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>21

Val Ile Leu Leu Asn Ser Ala Gly Gly Arg Pro Asp Ala Leu Glu

5 10 15

Met Glu Ala Asn Lys Gly Ala Gly Lys Lys Ser Lys Ser Ala Lys

20 25 30

Val Phe Ser Thr Leu Ser Trp Ser Trp Ser Phe Lys Ser Gln Gln

35 40 45

Ala Asn Ser Glu Ser Thr Val Leu Asn Ser Ser Ala Ser Gly Tyr

50 55 60

Gly Tyr His Gly Lys Thr Leu Glu Lys Leu Tyr Glu Glu Glu Glu

65 70 75

Lys Leu Tyr Lys Leu Ile Lys Asp Glu Glu Phe Ala Arg Leu Gln

80 85 90

Tyr Lys Lys His Val Ser Val Leu Gln Lys Leu Glu Ser Gly Glu

95 100 105

His Asp Arg Leu His Ala Glu Arg Val Arg Asp Ala Ile Glu Glu

110 115 120

Leu Gln Thr Arg Ile Ile Ser Leu Glu Glu Ala Val Ser Leu Ala

125 130 135

Cys Phe Ser Ile Ser Asn Leu Arg Asp Glu Glu Leu Tyr Pro Gln

140 145 150

Ile Ile Glu Leu Ser Ala Gly Leu Val His Met Trp Lys Asn Met

155 160 165

His Glu Cys His Gln Val Gln Asn His Ile Ala Gln Gln Leu Ser

170 175 180

Leu Ile Gly Asn Arg Pro Gly Ser Glu Pro Thr Ser Asn Ile His

185 190 195

Tyr Gln Ala Thr Ser Gln Leu Glu Ile Glu Val Ser Gly Trp His

200 205 210

Thr Ala Phe Cys Asn Leu Ile Thr Leu Gln Arg Glu Trp Ile Ser

215 220 225

Ile Leu Asn Gln Trp Ile Ser Leu Thr Asp Cys Leu Pro Asp Asp

230 235 240

Gly Gly Leu Met Arg Ser Ser Ser Gly Ile Arg Ser Leu Ser Lys

245 250 255

Glu Leu Gln His Ala Leu Asp Arg Leu Pro Glu Lys Val Ala Ala

260 265 270

Glu Ala Ile Lys Thr Phe Met Ser Ile Ile His Ser Ile Val Val

275 280 285

Gln Gln Ser Glu Glu Arg Gln Leu Lys Lys Lys Ser Asp Asn Met

290 295 300

Asp Ser Lys Phe Gln Thr Gln Leu Glu Lys His Ser Asp Asn Ser

305 310 315

Ile Gln Asn Ser Ala QGln Pro Pro Asn Lys Asn His Tyr Ser Val

320 325 330

Ser Lys Asn Glu Thr Lys Leu Asp Ala Phe Arg Lys Lys Val Glu

335 340 345

Glu Glu Lys Ala Arg Tyr Leu Thr Ser Val Arg Glu Ser Arg Ala

350 355 360

Met Thr Leu Asn Asn Leu Gln Thr Ala Leu Pro Asn Val Phe His

365 370 375

Ala Leu Glu Gla Phe Ser Gly Val Cys Val Gln Ala Phe Glu Gly

380 385 390

Ile Ser

<210>22

<211>361

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>22

Thr Ile Ile Leu Arg Leu Met Ile Asp Leu Lys Ala His Ala Ala

5 10 15

Val Leu Asn Arg Met Leu Ala Trp Glu Lys Lys Leu Tyr Asp Glu

20 25 30

Val Lys Ala Gly Glu Leu Met Lys Thr Asp Tyr Gln Lys Lys Val

35 40 45

Ser Leu Leu Gln Lys Gln Lys Lys Arg Gly Val Lys Leu Glu Thr

50 55 60

Leu Glu Lys Thr Lys Ala Ala Val Ser Gln Leu His Thr Arg Tyr

65 70 75

Ile Val Asp Met Gln Ser Met Asp Ser Thr Val Ser Glu Ile Asn

80 85 90

Arg Leu Arg Asp Arg Gln Leu Phe Pro Lys Leu Met Asp Leu Val

95 100 105

Asp Gly Met Ala Lys Meyt Trp Ser Ser Met Tyr Arg His His Arg

110 115 120

Ser Gln Phe Leu Ile Ile Ser Gly Ile Arg Gly Phe Glu Ile Pro

125 130 135

Pro Ile Pro Arg Glu Thr Thr Asp Ser His Tyr Ile Gln Thr Cys

140 145 150

Glu Leu Arg Asp Ile Val Arg Glu Trp His Thr Gln Phe Glu Lys

155 160 165

Leu Mey Asp Asn Gln Lys Ala Tyr Ile Arg Ala Leu Asn Ala Trp

170 175 180

Leu Lys Leu Asn Leu Ile Pro Ile Glu Ser Asn Ile Lys Glu Lys

185 190 195

Val Ser Ser Pro Pro Arg Leu Val Asp Pro Pro Ile Lys His Leu

200 205 210

Pro Arg Ala Trp His Asp Glu Leu Glu Arg Leu Pro Ile Glu Leu

215 220 225

Ala Lys Thr Ala Ile Lys Thr Phe Ala Glu Val Ile Ser Thr Ile

230 235 240

Val His Leu Gln Glu Glu Glu Val Asn Leu Arg Arg Arg Cys Asp

245 250 255

Glu Thr Arg Arg Asp Leu Asn Arg Lys Lys Ala Gln Phe Glu Asp

260 265 270

Trp His Gln Lys Tyr Leu Glu Arg Gln Thr Ala Glu Thr Gln Asn

275 280 285

Ile Asp Pro Val Glu Asp Arg Lys Arg Thr Ile Glu Glu Leu Glu

290 295 300

Ile Arg Leu Arg Glu Glu Glu Gly His His Leu Arg His Ala Arg

305 310 315

Gln Val Arg Glu Lys Ser Leu Ala Asn Leu Arg Thr His Leu Pro

320 325 330

Glu Leu Phe Arg Asn Met Ser Asp Phe Ala Tyr Phe Cys Lys Asp

335 340 345

Met Tyr Asn Asn Leu Arg Lys Thr Ala Ala Leu Ser Lys Asp Glu

350 355 360

Val

<210>23

<211>206

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>23

Val Ser Gly Trp His Thr Ala Phe Cys Asn Leu Ile Thr Ser Gln

5 10 15

Arg Glu Tyr Val Ser Ile Leu Ser Gln Trp Ile Gly Leu Thr Asp

20 25 30

Cys Leu Pro Asp Asp Gly Gly Leu Met Arg Ser Ser Ser Gly Ile

35 40 45

Leu Ser Leu Ser Glu Glu Leu Gln Arg Ala Leu Asp Arg Leu Pro

50 55 60

Glu Lys Val Ala Ala Glu Ala Ile Lys Thr Phe Met Ser Val Ile

65 70 75

His Ser Ile Val Val Gln Gln Ser Glu Glu Arg Gln Leu Lys Lys

80 85 90

Lys Ser Asn Asn Met Asp Ser Lys Phe Gln Thr Gln Leu Glu Lys

95 100 105

His Ser Glu Asn Thr Met Gln Asn Ser Ala Gln Pro Pro Asn Lys

110 115 120

Asn Asn Tyr Ser Ala Ser Lys Asn Gly Thr Lys Leu Asp Ala Phe

125 130 135

Ile Lys Gln Val Glu Glu Glu Lys Ala Arg Tyr Leu Thr Ser Val

140 145 150

Arg Val Ser Arg Ala Met Thr Leu Asn Ser Leu Gln Thr Ser Leu

155 160 165

Arg Asn Val Phe His Ala Leu Glu Gly Phe Ser Gly Val Cys Val

170 175 180

Gln Ala Phe Glu Gly Ile Ser Arg Cys Ser Glu Val Ala Val Ile

185 190 195

His Ser Gly Ala Ala Ser Pro Ala Ile Ser Ser

200 205

<210>24

<211>335

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>24

Met Ala Asp Ala Ser Ser Arg Thr Asp Thr Ser Thr Val Val Asp

5 10 15

Met Asp Asp Lys Asn Gln Arg Leu Glu Asn Gly Gln Ser Gly Ala

20 25 30

Met Val Pro Ala Ser Asn Ser Ser Asp Arg Ser Asp Arg Ser Asp

35 40 45

Lys Pro Met Asp Gln Lys Val Leu Arg Arg Phe Ala Gln Asn Arg

50 55 60

Glu Ala Ala Arg Lys Ser Arg Leu Arg Lys Lys Ala Tyr Val Gln

65 70 75

Gln Leu Glu Ser Ser Lys Leu Lys Leu Ala Ser Leu Glu Gln Glu

80 85 90

Leu Gln Lys Ala Arg Gln Gln Gly Ile Phe Ile Ser Ser Ser Gly

95 100 105

Asp Gln Thr His Thr Met Ser Gly Asn Gly Ala Met Thr Phe Gly

110 115 120

Leu Glu Tyr Ser Arg Trp Gln Glu Glu Gln Asn Lys Gln Ile Asn

125 130 135

Glu Leu Arg Thr Ala Val Asn Ala His Ala Ser Asp Ser Asp Leu

140 145 150

Arg Leu Ile Val Asp Gly Ile Met Ala His Tyr Asp Glu Ile Phe

155 160 165

Arg Leu Lys Gly Ile Ala Ala Lys Ala Asp Val Phe His Ile Leu

170 175 180

Ser Gly Met Trp Lys Thr Pro Ala Glu Arg Cys Phe Leu Trp Leu

185 190 195

Gly Gly Phe Arg Ser Ser Glu Leu Leu Lys Leu Leu Val Asn Gln

200 205 210

Leu Glu Pro Leu Thr Glu Gln Gln Leu Met Gly Leu Ser Asn Leu

215 220 225

Gln Gln Ser Ser Gly Gln Ala Glu Asp Ala Leu Ser Gln Gly Met

230 235 240

Glu Ala Leu His Gln Ser Leu Ala Gln Thr Leu Ala Gly Ser Ler

245 250 255

Gly Pro Ser Gly Ser Ser Trp Asn Val Ala Asn Tyr Met Gly Ser

260 265 270

Met Ala Met Ala Met Gly Lys Leu Gly Thr Leu Gln Asn Phe Leu

275 280 285

Pro Gln Ala Asp Asn Leu Arg Gln Gln Thr Leu His Gln Met Gln

290 295 300

Arg Ile Leu Thr Ile Arg Gln Ala Ala Arg Ala Leu Leu Ala Ile

305 310 315

His Asp Tyr Phe Ser Arg Leu Arg Ala Leu Ser Ser Leu Trp Leu

320 325 330

Ala Arg Pro Arg Glu

335

<210>25

<211>214

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>25

Met Glu Gln Gln Ala Gln Leu Arg Asp Ala Leu Asn Asp Ala Leu

5 10 15

Arg Glu Glu Val Glu Arg Leu Lys Ile Ala Thr Gly Glu Met Ser

20 25 30

Lys Ser Asn Asn Glu His Phe Asn Met Gly Met Gln His Val Ser

35 40 45

Tyr Ser Pro Ser Phe Phe Gln Leu Ser Glu Gln His Thr Met Gln

50 55 60

Gln His Gly Asn Ile Gln Leu Pro His His Phe Gln Gln Pro

65 70 75

Pro Leu Asn Ala Pro Ser His Gln Met Leu Ser His Pro Asn Ser

80 85 90

Leu Ser Asn Met Val Gln Gln Glu Ser Leu Gly Arg Leu Gln Gly

95 100 105

Leu Asp Ile Gly Lys Gly Ser Met Ala Val Lys Ser Glu Ala Glu

110 115 120

Val Val Val Lys Ser Glu Gly Ser Ser lle Ser Ala Gly Glu Ser

125 130 135

Asn Thr Thr Phe Leu Tyr Asn Thr Ser Ala Phe Glu Ala Phe Val

140 145 150

Trp Phe Arg Val Leu Leu Phe Ile Val His Pro Thr Ala Ser Ser

155 160 165

Phe Pro Val Val Phe Leu Phe His Ser Leu His Leu Val Thr Ile

170 175 180

Asp Arg Leu Cys Arg Ser Leu Leu Ser Trp Trp Leu Ile Leu Gly

185 190 195

His Trp Cys Val Gly Phe Glu Phe Val Met Cys Val Val Trp Leu

200 205 210

Val Val Ser Ser

<210>26

<211>168

<212>PRT

＜213〉corn kind Zea (Zea mays L.)

<400>26

Met Gly Thr Leu Ser Arg Ser Gly Ser His Thr Leu Lys Thr Asn

5 10 15

Arg Lys Leu Phe Gln Asn Gly Ser Thr Gly Leu Ile Thr Pro Gln

20 25 30

Ser Ser Gly Leu Ile Glu Arg Glu Arg Val Arg Thr Lys Ser Gln

35 40 45

Pro Leu Asp Pro Lys Ala Thr Ser Asn Gla Asp Leu His Thr Met

50 55 60

Asp Glu Tyr Gln Ser Val Ile Met Gln Leu Arg Asp Glu Asn Ser

65 70 75

Ser Leu Lys Glu Arg Leu Ser Lys Met Glu Glu Ser Met Ser Gln

80 85 90

Glu Leu Val Val Glu Arg Asp Lys Asn Lys Ser Leu Thr Glu Arg

95 100 105

Val Glu Asp Leu Gln Arg Gln Leu Glu Ser Ala Thr Lys Glu Gln

110 115 120

Glu Ala Leu Ile Asp Ile Phe Ser Glu Glu Arg Asn Arg Arg Asp

125 130 135

Gln Glu Glu Glu Ser Leu Arg Arg Lys Leu Lys Asp Ala Ser Ser

140 145 150

Thr Ile Gln Asp Leu Leu Glu Gln Leu Asn Val Ala Arg Lys Gly

155 160 165

Arg Lys Val

Claims (5)

1. grow relevant bZIP transcription factor MSBZIP1-13 with corn kernel, it is characterized in that this transcription factor is the protein with amino acid residue sequence of sequence 14-26 in the sequence table, or the amino acid residue sequence of sequence 14-26 is passed through replacement, disappearance or the interpolation of one or several amino-acid residue and has identical active by sequence 14-26 deutero-protein with the amino acid residue sequence of sequence 14-26.

2. according to claim 1 and corn kernel are grown relevant bZIP transcription factor MSBZIP1-13, it is characterized in that this transcription factor is the protein with amino acid residue sequence of sequence 14-26 in the sequence table.

3. according to claim 1 and corn kernel are grown the encoding gene of relevant bZIP transcription factor MSBZIP1-13, it is characterized in that this encoding gene has one of following nucleotide sequences:

1) dna sequence dna of 1-13 in the sequence table sequence;

2) with sequence table in the nucleotide sequence that limits of sequence 1-13 have the homology 90% or more and the identical function protein DNA sequence of encoding.

4. according to claim 3 and corn kernel are grown the encoding gene of relevant bZIP transcription factor MSBZIP1-13, it is characterized in that this encoding gene has the dna sequence dna of 1-13 in the sequence table sequence.

5. according to claim 1 and corn kernel are grown the expression of relevant bZIP transcription factor MSBZIP1-13, and its sequence signature is the continuous expression in the seed growth course of MSBZIP2, MSBZIP4, MSBZIP6, MSBZIP7, MSBZIP8, MSBZIP10, MSBZIP11, MSBZIP12 and MSBZIP13 in this transcription factor; MSBZIP1, MSBZIP3, MSBZIP5, MSBZIP9 are phasic specificity to express in growth course.

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