CN101078722A - Gold marked reagent quantitative determination device and method - Google Patents
Gold marked reagent quantitative determination device and method Download PDFInfo
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- CN101078722A CN101078722A CN 200610026828 CN200610026828A CN101078722A CN 101078722 A CN101078722 A CN 101078722A CN 200610026828 CN200610026828 CN 200610026828 CN 200610026828 A CN200610026828 A CN 200610026828A CN 101078722 A CN101078722 A CN 101078722A
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Abstract
The invention discloses a quantitative detection device of gold-labeled reagent, characterized in that it comprises microcomputer system and following parts connected with it comprising scanning module, transforming module, data storing module, reagent-strip slot, IC-card slot, keyboard, display and micro printer. Optical fiber probe transfers light reflected by reagent strip to photoelectric converter and the light is transformed into electric signal. After the electric signal passes through A/D converter it is transformed into digital signal. It is contrasted with standard curve in IC card to obtain the concentration value, store, display and print result. The invention discloses a measuring method for measuring gold-labeled strip by the device at the same time. The invention solves the shortage that gold-labeled reagent can not be used to quantitative detection but be used to qualitative detection. It not only obtains quantitative result but also can improve measuring sensitivity and objectivity of measuring result. It is provided with convenient usage and quick measurement.
Description
Technical field
The present invention relates to a kind of medical immunology diagnostic analysis instrument, specifically relate to a kind of device that can carry out detection by quantitative, the invention still further relates to a kind of assay method that uses this device to detect gold marked reagent.
Background technology
Colloidal gold immunity chromatography is the most frequently used a kind of express-analysis technology in the biomedical at present check, its feature is single part mensuration, and is simple, fast, except that commercially available reagent without any need for instrument and equipment, a few minutes result that can detect by an unaided eye, the holding time is long.The varieties of reagent of colloidal gold immunity chromatography is also abundant day by day, nearly hundred kinds have been reached at present, the product range of application comprises microorganism, viral antigen or antibody test, hormone detection, tumor markers detection, drugs heroin, morphine, head-shaking pill, ketamine detection etc., develops very rapid.
Because present colloidal gold immunity chromatography reagent clinical examination sentence read result is mainly by range estimation, therefore colloidal gold immunity chromatography is measured and generally can only be used for qualitatively judging, range of application has been subjected to very big restriction, the invention provides a kind of device that can carry out detection by quantitative to gold marked reagent, make gold marked reagent upgrade to detection by quantitative from qualitative detection, the use field is very extensive, and very big using value and technological value are arranged.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of device that can carry out detection by quantitative to gold marked reagent.
In order to solve the problems of the technologies described above, the present invention adopts following technical scheme: a kind of gold marked reagent detection by quantitative device comprises microcomputer system and coupled following each several part:
Scan module is made of scan control circuit, mechanical driving device and fibre-optical probe, is used for reagent strip is scanned;
Modular converter is made of photoelectric commutator and A/D converter, is used for the colour developing of reagent strip is converted to digital signal;
Data memory module is used to store detected data;
The reagent strip slot is used to insert the test agent bar;
The IC-card slot is used to insert the IC-card that stores test agent bar quality control band concentration and typical curve;
Keyboard, display and mini-printer.
Fibre-optical probe to photoelectric commutator, is converted to electric signal after A/D converter is converted to digital signal with the light transmission of reagent strip reflection, compares with typical curve in the IC-card, draws concentration value, and the result is stored, shows and prints.The invention solves gold marked reagent and can only be used for qualitative detection, and can not be used for the defective of detection by quantitative, not only can provide quantitative result, and can improve the objectivity of detection sensitivity and testing result.Pick-up unit of the present invention is easy to use, detects rapidly.
Preferably, also comprise a PORT COM, be used for being connected with PC.So just testing result can be passed to PC.
Preferably, also comprise a signal processing circuit, be used for to the photosignal that scanning produces amplify, compensation deals.
Another technical matters that the present invention will solve provides a kind of assay method that utilizes said apparatus the gold marked reagent bar to be carried out detection by quantitative.
In order to solve this technical problem, assay method of the present invention may further comprise the steps:
A) will insert in the IC-card slot with the corresponding IC-card of test agent bar, and the energising start;
B) the test agent bar is inserted the reagent strip slot, scan module starts scanning automatically;
C) fibre-optical probe arrives modular converter with the optical signal transmission that scans, and after opto-electronic conversion and A/D conversion, is transferred to microcomputer system;
D) microcomputer system is compared the typical curve in detected signal and the IC-card, calculates the concentration value that detects band;
E) output result.
Assay method of the present invention is easy and simple to handle, testing result is accurate.
Description of drawings
Below in conjunction with the drawings and specific embodiments the present invention is described in further detail.
Fig. 1 is the composition frame chart of gold marked reagent detection by quantitative device of the present invention.
Embodiment
As shown in Figure 1, gold marked reagent detection by quantitative device of the present invention comprises microcomputer system and coupled scan module, modular converter, data memory module, reagent strip slot, IC-card slot, keyboard, LCD display and mini-printer.Wherein microcomputer system adopts the 89C55MCU single-chip microcomputer, and scan module is made of scan control circuit, mechanical driving device and fibre-optical probe, can scan reagent strip; Modular converter is made of photoelectric commutator and A/D converter, fibre-optical probe arrives photoelectric commutator with the colour developing optical signal transmission of reagent strip, be converted to electric signal, in order to make testing result more accurate, added a signal processing circuit, be used for electric signal is amplified and the background compensation processing, this electric signal is converted to digital signal through A/D converter again.This device also is equipped with PORT COM, can be connected with PC, and testing result is imported PC.
When detecting, will insert in the IC-card slot with the corresponding IC-card of test agent bar earlier, store test agent bar quality control band concentration and typical curve in the IC-card, energising start then; The test agent bar is inserted the reagent strip slot, and scan module starts scanning automatically; Fibre-optical probe arrives modular converter with the optical signal transmission that scans, and after opto-electronic conversion and A/D conversion, is transferred to microcomputer system; Microcomputer system is compared the typical curve in detected signal and the IC-card, calculates the concentration value that detects band; The output result, this testing result also is stored in the data memory module simultaneously.
Claims (4)
1. gold marked reagent detection by quantitative device is characterized in that: comprise microcomputer system and coupled following each several part:
Scan module is made of scan control circuit, mechanical driving device and fibre-optical probe, is used for reagent strip is scanned;
Modular converter is made of photoelectric commutator and A/D converter, is used for the colour developing of reagent strip is converted to digital signal;
Data memory module is used to store detected data;
The reagent strip slot is used to insert the test agent bar;
The IC-card slot is used to insert the IC-card that stores test agent bar quality control band concentration and typical curve;
Keyboard, display and mini-printer.
2. a kind of gold marked reagent detection by quantitative device according to claim 1 is characterized in that: also comprise a PORT COM, be used for being connected with PC.
3. a kind of gold marked reagent detection by quantitative device according to claim 1 is characterized in that: also comprise a signal processing circuit, be used for to the photosignal that scanning produces amplify, compensation deals.
4. one kind is used the described gold marked reagent detection by quantitative of claim 1 device to carry out method for measuring, it is characterized in that may further comprise the steps:
A) will insert in the IC-card slot with the corresponding IC-card of test agent bar, and the energising start;
B) the test agent bar is inserted the reagent strip slot, scan module starts scanning automatically;
C) fibre-optical probe arrives modular converter with the optical signal transmission that scans, and after opto-electronic conversion and A/D conversion, is transferred to microcomputer system;
D) microcomputer system is compared the typical curve in detected signal and the IC-card, calculates the concentration value that detects band;
E) output result.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610026828 CN101078722A (en) | 2006-05-24 | 2006-05-24 | Gold marked reagent quantitative determination device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610026828 CN101078722A (en) | 2006-05-24 | 2006-05-24 | Gold marked reagent quantitative determination device and method |
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| CN101078722A true CN101078722A (en) | 2007-11-28 |
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| CN 200610026828 Pending CN101078722A (en) | 2006-05-24 | 2006-05-24 | Gold marked reagent quantitative determination device and method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183570A (en) * | 2011-02-14 | 2011-09-14 | 北京软测科技有限公司 | Realization method for improving blood detection accuracy and detector |
| CN103197060A (en) * | 2013-03-24 | 2013-07-10 | 湖南远泰生物技术有限公司 | Autonomous recognition, positioning and quantitative detecting system and detecting method for colloidal gold immunochromatographic boxes |
| CN103487586A (en) * | 2013-09-04 | 2014-01-01 | 石家庄洹众生物科技有限公司 | Testing device for quantitatively detecting soluble growth stimulation expression protein 2 |
| CN104020286A (en) * | 2014-06-10 | 2014-09-03 | 武汉璟泓万方堂医药科技股份有限公司 | Quantitative detection instrument and detection method of immunochromatography test strip |
| CN105842218A (en) * | 2016-05-24 | 2016-08-10 | 天津倍肯生物科技有限公司 | Nanometer microfluidic accurate and quantitative fluoroimmunoassay detection platform |
| CN106066401A (en) * | 2014-03-07 | 2016-11-02 | 高平 | Biomarker VWF and ADAMTS13 and the purposes in liver cirrhosis diagnosis reagent thereof |
| CN110320374A (en) * | 2018-03-30 | 2019-10-11 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of reagent principal curve scan method and sample analyser |
-
2006
- 2006-05-24 CN CN 200610026828 patent/CN101078722A/en active Pending
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102183570A (en) * | 2011-02-14 | 2011-09-14 | 北京软测科技有限公司 | Realization method for improving blood detection accuracy and detector |
| CN103197060A (en) * | 2013-03-24 | 2013-07-10 | 湖南远泰生物技术有限公司 | Autonomous recognition, positioning and quantitative detecting system and detecting method for colloidal gold immunochromatographic boxes |
| CN103487586A (en) * | 2013-09-04 | 2014-01-01 | 石家庄洹众生物科技有限公司 | Testing device for quantitatively detecting soluble growth stimulation expression protein 2 |
| CN103487586B (en) * | 2013-09-04 | 2015-07-15 | 石家庄洹众生物科技有限公司 | Testing device for quantitatively detecting soluble growth stimulation expression protein 2 |
| CN106066401A (en) * | 2014-03-07 | 2016-11-02 | 高平 | Biomarker VWF and ADAMTS13 and the purposes in liver cirrhosis diagnosis reagent thereof |
| CN106066401B (en) * | 2014-03-07 | 2018-11-23 | 重庆早柒天生物科技股份有限公司 | Biomarker VWF and ADAMTS13 and its purposes in liver cirrhosis diagnosis reagent |
| CN104020286A (en) * | 2014-06-10 | 2014-09-03 | 武汉璟泓万方堂医药科技股份有限公司 | Quantitative detection instrument and detection method of immunochromatography test strip |
| CN104020286B (en) * | 2014-06-10 | 2016-08-31 | 武汉璟泓万方堂医药科技股份有限公司 | A kind of immuno-chromatographic test paper strip instrument and detection method |
| CN105842218A (en) * | 2016-05-24 | 2016-08-10 | 天津倍肯生物科技有限公司 | Nanometer microfluidic accurate and quantitative fluoroimmunoassay detection platform |
| CN105842218B (en) * | 2016-05-24 | 2018-01-16 | 天津倍肯生物科技有限公司 | A kind of micro-fluidic accurate quantification fluorescence immunoassay detection platform of nanometer |
| CN110320374A (en) * | 2018-03-30 | 2019-10-11 | 深圳迈瑞生物医疗电子股份有限公司 | A kind of reagent principal curve scan method and sample analyser |
| CN110320374B (en) * | 2018-03-30 | 2024-03-01 | 深圳迈瑞生物医疗电子股份有限公司 | Reagent main curve scanning method and sample analyzer |
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Open date: 20071128 |