CN101062109A - Medicinal composition for treating dysmenorrhea and preparation process thereof - Google Patents
Medicinal composition for treating dysmenorrhea and preparation process thereof Download PDFInfo
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- CN101062109A CN101062109A CNA2006100789713A CN200610078971A CN101062109A CN 101062109 A CN101062109 A CN 101062109A CN A2006100789713 A CNA2006100789713 A CN A2006100789713A CN 200610078971 A CN200610078971 A CN 200610078971A CN 101062109 A CN101062109 A CN 101062109A
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- angelicae sinensis
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Abstract
The invention discloses a medicinal compound to cure menalgia and preparing craft, which is characterized by the following: producing the compound with angelica polysaccharide and corydalis ambigua total alkalinity; testing the angelica polysaccharide with fenol-sulfuric acid method; setting the content of antacidin more than 50%; testing corydalis ambigua total alkalinity with ultraviolet spectrophoto method; setting the content of corydalis B more than 50%; choosing the raw material medicine; adding into normal findings; producing to clinical oral liquid preparation, mixture, capsule, pill, granula, tablet, intramuscular injection or intravenous injection with normal craft; dissolving the raw material with injecting water; adding into activated char; stirring; filtering; adjusting the pH value at 4. 0-6. 0; filtering; loading; sterilizing; getting the product. This invention also relates to the usage of the medicinal compound to prepare menalgia medicine.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition and preparation technology thereof, particularly a kind of pharmaceutical composition and preparation technology thereof who treats dysmenorrhea.
Background technology
Dysmenorrhea be the women in menstrual period and front and back thereof, lower abdomen or flank pain appear, even pain and waist sacrum, whenever send out with menstrual cycle, severe patient can be with many syndromes, bring adverse effect to work and life.The medicine of treatment dysmenorrhea has many reports and uses at present, and pair indication and application characteristic thereof are respectively arranged.But the aspects such as range of the occupation mode of medicine and applicable object scope remain further to be improved.
Radix Angelicae Sinensis polysaccharide is the total polysaccharides of extracting from natural medicinal raw material Radix Angelicae Sinensis.When the dry root that is classified as Umbelliferae archangel Radix Angelicae Sinensis, have effects such as promoting blood circulation and hemostasis, adjusting meridian and stopping leukorrhea.Radix Angelicae Sinensis polysaccharide is one of main component in the Radix Angelicae Sinensis, and domestic and international in recent years composition Study and the pharmaceutical research to Radix Angelicae Sinensis polysaccharide had new progress.Radix Angelicae Sinensis polysaccharide is to the obvious effect of body immune system, hemopoietic system, and the good efficacy to antitumor, radioresistance damage has tangible analgesia, anticoagulation and anastalsis.Radix Angelicae Sinensis polysaccharide brings out the female mice hysterospasm with the simulation dysmenorrhes to diethylstilbestrol and oxytocin simultaneously, can effectively resist estrogen and bring out the caused pain reaction of dysmenorrhea.Rhizoma Corydalis total alkaloids is the total alkaloids that extracts in the natural medicinal raw material Rhizoma Corydalis.Rhizoma Corydalis is the dry tuber of papaveraceae plant corydalis Corydalis yanhusuo W.T.Wang.Rhizoma Corydalis total alkaloids has significant analgesia role, and the antagonism oxytocin causes that the contractile response in isolated rat uterus has pharmacologically active.But Radix Angelicae Sinensis polysaccharide and Rhizoma Corydalis total alkaloids the two synergism and the treatment dysmenorrhea effective proportion relation do not appear in the newspapers as yet.
Summary of the invention
The object of the invention is to disclose a kind of pharmaceutical composition and preparation technology thereof.
The present invention seeks to be achieved through the following technical solutions:
The crude drug of pharmaceutical composition of the present invention consists of:
Radix Angelicae Sinensis polysaccharide 1-9 weight portion Rhizoma Corydalis total alkaloids 1-9 weight portion.
Above-mentioned two flavor crude drug preferred weight proportionings are as follows:
Radix Angelicae Sinensis polysaccharide 2 weight portion Rhizoma Corydalis total alkaloidss 8 weight portions;
Radix Angelicae Sinensis polysaccharide 5 weight portion Rhizoma Corydalis total alkaloidss, 5 weight portions or
Radix Angelicae Sinensis polysaccharide 8 weight portion Rhizoma Corydalis total alkaloidss 2 weight portions.
Above-mentioned Radix Angelicae Sinensis polysaccharide adopts the phenolsulfuric acid method to measure, content with glucose meter greater than 50%; Rhizoma Corydalis total alkaloids adopts determined by ultraviolet spectrophotometry, content in tetrahydropalmatine greater than 50%.
In the pharmaceutical composition crude drug of the present invention Radix Angelicae Sinensis polysaccharide can but be not limited to following method and make:
Radix Angelicae Sinensis is with the distilled water reflux, extract, of 6-10 times of weight portion 2-4 time, and each 3-5h is after 2-4 the extracting solution merging, distilling under reduced pressure concentrates, and after the concentrated solution pre-cooling, adds the ethanol of 2-4 times of parts by volume, stir, after leaving standstill, slightly carried polysaccharide, slightly carried the polysaccharide dissolved in distilled water with the High speed refrigerated centrifuge collecting precipitation, under the 3-5 ℃ of condition to distill water dialysis 2-4 days, centrifugal removal precipitation, the supernatant lyophilization, promptly.
The method for optimizing of Radix Angelicae Sinensis polysaccharide is as follows in the pharmaceutical composition crude drug of the present invention:
Radix Angelicae Sinensis is with the distilled water reflux, extract, of 8 times of weight portions 3 times, and each 4h is after 3 times extracting solution merges, distilling under reduced pressure concentrates, and after the concentrated solution pre-cooling, adds the ethanol of 3 times of parts by volume, stir, after leaving standstill, slightly carried polysaccharide, slightly carried the polysaccharide dissolved in distilled water with the High speed refrigerated centrifuge collecting precipitation, under 4 ℃ of conditions to distill water dialysis 3 days, centrifugal removal precipitation, the supernatant lyophilization, promptly.
In the pharmaceutical composition crude drug of the present invention Rhizoma Corydalis total alkaloids can but be not limited to following method and make:
Get the Rhizoma Corydalis powder and put in the extractor, moistening with strong aqua ammonia, 5-7 times of parts by volume chloroform extraction 2-4 hour filters, the filtrate water bath method, promptly.
The method for optimizing of Rhizoma Corydalis total alkaloids is as follows in the pharmaceutical composition crude drug of the present invention:
Get the Rhizoma Corydalis powder and put in the extractor, moistening with strong aqua ammonia, 6 times of parts by volume chloroform extraction 3 hours filter, the filtrate water bath method, promptly.
The pass of above-mentioned weight portion and parts by volume is the relation of gram and milliliter.
The preparation of Radix Angelicae Sinensis polysaccharide, Rhizoma Corydalis total alkaloids crude drug can be but be not limited to above method.Radix Angelicae Sinensis polysaccharide can be a water extract-alcohol precipitation, and process is removed oils and fats and protein gained polysaccharide, or adopts water extract-alcohol precipitation gained polysaccharide, through the Radix Angelicae Sinensis polysaccharide of ultrafilter membrane gained certain molecular weight scope; Rhizoma Corydalis total alkaloids can be the Rhizoma Corydalis total alkaloids that solvent cold-maceration, ethanol extraction method, ether or chloroform extraction method etc. obtain.
Get the above-mentioned composition crude drug, add conventional adjuvant,, make clinical oral liquid, syrup preparation, capsule, pill, granule, tablet, intramuscular dose or the intravenous injection accepted according to common process.
The preparation technology of medicine composition injection of the present invention is as follows:
Get pharmaceutical composition crude drug of the present invention, with the dissolving of 75-200 parts by volume water for injection, add the 0.05-0.2% active carbon and stirred 3-7 minute, filter, water for injection adds to the 450-1000 parts by volume, regulates pH value to 4.0-6.0, filter, and fill, sterilization, promptly.
The preferred for preparation technology of medicine composition injection of the present invention is as follows:
Get pharmaceutical composition crude drug of the present invention, with the dissolving of 100 parts by volume waters for injection, add 0.1% active carbon and stirred 5 minutes, filter, water for injection adds to 500 parts by volume, regulates pH value to 5.0, filter, and fill, sterilization, promptly.
Compositions of the present invention has therapeutical effect to aspects such as dysmenorrheas, obviously is better than single component wherein aspect dysmenorrhea treatment, has obvious synergistic, potentiation.Pharmacodynamic experiment shows: pharmaceutical composition of the present invention has good analgesic activity and to the contraction inhibitory action in uterus.Simultaneously, pharmaceutical composition of the present invention is except that having more available variety range and leeway to dysmenorrhea treatment the time, also simple because of its composition, effective ingredient is concentrated, thereby the use amount of medicine is reduced relatively, and after being made into the medicine of several formulations form, can also realize that the scope of occupation mode and/or patient's object is more extensive, the purpose that helps promoting the use of on a large scale.
Following experimental example and embodiment are used to further specify but are not limited to the present invention.
Experimental example 1 analgesic experiment
1. diethylstilbestrol and oxytocin are brought out the influence of Mouse Uterus spasm
60 of female mice hysterospasm ♀ mices are brought out in diethylstilbestrol and oxytocin, are divided into 6 groups.Radix Angelicae Sinensis polysaccharide: Rhizoma Corydalis total alkaloids is the present composition of 4: 1 (sample 1), 1: 1 (sample 2), 1: 4 (sample 3) different proportion form, press 120mg (effective ingredient total amount)/kg and irritate stomach, matched group is the Radix Angelicae Sinensis polysaccharide and the Rhizoma Corydalis total alkaloids of equivalent, carried out the contrast experiment respectively, compared between organizing with the F check.Experimental result is as shown in table 1.1h gives Rhizoma Corydalis total alkaloids, Radix Angelicae Sinensis polysaccharide, medicine of the present invention through irritating stomach before the experiment, and model control group waits the capacity normal saline.Observe in mouse writhing response latency and the 30min and turn round the body number of times.
The influence of Mouse Uterus spasm is brought out in table 1 pair diethylstilbestrol and oxytocin
| Group | Dosage/mgkg -1 | Writhing response | |
| Incubation period/min | Number of times/30min | ||
| 3 groups in 2 groups of samples of 1 group of sample of model group Radix Angelicae Sinensis polysaccharide Rhizoma Corydalis total alkaloids sample | - 120 120 120 120 120 | 15.4±5.2 22.3±7.5 * 18.4±5.6 * 28.3±5.2 ** 31.7±4.8 ** 29.2±3.9 ** | 6.8±3.8 1.3±1.1 ** 2.3±1.7 ** 1.3±1.5 ** 1.1±1.8 ** 1.5±2.1 ** |
Compare with model group: *: P＜0.05, * *: P＜0.01
The experimental result of table 1 shows that the laboratory sample group of the present invention of two kinds of active component different proportion forms all can make the mouse writhing response latency obviously prolong, and turns round the body number of times and obviously reduces (the P value all＜0.01).The result of table 1 also demonstrates, the effect that the laboratory sample of three groups of different proportion forms brings out the Mouse Uterus spasm to diethylstilbestrol and oxytocin is basic identical, and the influence that the Mouse Uterus spasm is brought out in diethylstilbestrol and oxytocin of sample 1, sample 2, sample 3 all is better than the Radix Angelicae Sinensis polysaccharide group and the Rhizoma Corydalis total alkaloids group of single component, illustrate that medicine of the present invention has obvious synergistic function, and sample 2 is the best of breed ratio.
2. the influence of female mice writhing response due to the Dichlorodiphenyl Acetate
Laboratory sample is respectively Radix Angelicae Sinensis polysaccharide: Rhizoma Corydalis total alkaloids is the present composition of 4: 1 (sample 1), 1: 1 (sample 2), 1: 4 (sample 3) different proportion form, press 120mg (effective ingredient total amount)/kg and irritate stomach, matched group be equivalent Radix Angelicae Sinensis polysaccharide, Rhizoma Corydalis total alkaloids and etc. the capacity normal saline.1hip 1% acetic acid 0.2mL/ only observes in administration mouse writhing response latency and the 30min and turns round the body number of times after the administration, and calculates the protection percentage rate.
The influence of female mice writhing response due to table 2 Dichlorodiphenyl Acetate
| Group | Dosage/mgkg -1 | Writhing response | ||
| Incubation period/min | Number of times/30min | Protective rate/% | ||
| 3 groups in 2 groups of samples of 1 group of sample of model group Radix Angelicae Sinensis polysaccharide Rhizoma Corydalis total alkaloids sample | - 120 120 120 120 120 | 5.4±1.2 12.3±2.4 ** 152±3.7 ** 18.4±3.4 *** 21.5±4.7 *** 18.2±3.5 *** | 32.8±6.8 15.3±5.1 ** 16.8±5.3 * 13.1±2.6 ** 9.4±4.1 *** 11.2±3.7 ** | 53.4 48.8 60.1 71.3 65.8 |
Compare with model group: *: P＜0.05, * *: P＜0.01, * * *: P＜0.001
The experimental result of table 2 shows that the laboratory sample group of the present invention of two kinds of active component different proportion forms all can make the mouse writhing response latency obviously prolong, and turns round the body number of times and obviously reduces, and turns round the body protective rate and obviously improves (the P value all＜0.01).The result of table 2 also demonstrates, the effect of female mice writhing response is basic identical due to the laboratory sample Dichlorodiphenyl Acetate of three groups of different proportion forms, and the influence of female mice writhing response all is better than the Radix Angelicae Sinensis polysaccharide group and the Rhizoma Corydalis total alkaloids group of single component due to the Dichlorodiphenyl Acetate of sample 1, sample 2, sample 3, illustrates that pharmaceutical composition of the present invention has obvious synergistic function.
3. to the influence of hot plate method analgesic activity
Laboratory sample is respectively Radix Angelicae Sinensis polysaccharide: Rhizoma Corydalis total alkaloids is the present composition of 4: 1 (sample 1), 1: 1 (sample 2), 1: 4 (sample 3) different proportion form, press 120mg (effective ingredient total amount)/kg and irritate stomach, matched group be equivalent Radix Angelicae Sinensis polysaccharide, Rhizoma Corydalis total alkaloids and etc. the capacity normal saline.Experimentize behind the 1h after the administration, the variation of the threshold of pain respectively organized in record, calculates the threshold of pain and improve percentage rate.If sufficient reaction do not occur licking in the 60S, 60S is counted in the threshold of pain.
The influence of table 3 pair hot plate method analgesic activity
| Group | Dosage/mgkg -1 | The preceding threshold of pain time/s of administration | Threshold of pain time/s after the administration | Threshold of pain raising rate/% |
| 3 groups in 2 groups of samples of 1 group of sample of model group Radix Angelicae Sinensis polysaccharide Rhizoma Corydalis total alkaloids sample | - 120 120 120 120 120 | 20.1±4.7 21.3±5.2 21.4±3.8 22.3±5.1 21.8±4.2 22.6±4.1 | 19.9±5.1 29.2±4.6 31.3±2.7 35.4±3.0 37.1±5.4 36.2±4.3 | 1.0 37.1 * 46.3 * 58.7 ** 70.2 *** 60.2 ** |
Compare with model group: *: P＜0.05, * *: P＜0.01, * * *: P＜0.001
The experimental result of table 3 shows, the laboratory sample group of the present invention of two kinds of active component different proportion forms all can significantly postpone mice and (P＜0.05) occur licking the sufficient response time, and the threshold of pain is improved percentage rate and obviously improved.The result of table 3 also demonstrates, the laboratory sample of three groups of different proportion forms is basic identical to the effect of hot plate method analgesic activity, and the influence to the hot plate method analgesic activity of sample 1, sample 2, sample 3 all is better than the Radix Angelicae Sinensis polysaccharide group and the Rhizoma Corydalis total alkaloids group of single component, illustrates that pharmaceutical composition of the present invention has obvious synergistic function.
The influence of 2 pairs of ♀ mices of experimental example isolated uterine spontaneous activity
Laboratory sample is respectively Radix Angelicae Sinensis polysaccharide: Rhizoma Corydalis total alkaloids is the present composition of 4: 1 (sample 1), 1: 1 (sample 2), 1: 4 (sample 3) different proportion form.Healthy ♀ unpregnancy mice, body weight 20-24g presses the 2mg/kgSC diethylstilbestrol injection before the experiment, and continuous 2d is to improve the sensitivity of uterus to medicine.During experiment mice is taken off neck and put to death, cut open rapidly and get the uterus, place immediately to fill through gaseous mixture (95%O
2+ 5%CO
2) in the saturated Rockwell liquid of %, careful periuterine connective tissue and the fatty tissue removed, make the uterus specimen that is about 1cm, specimen one end is fixed on the little hook of specimen plate, place in the bath that fills 20ml Rockwell liquid, the other end links to each other with tonotransducer, and water-bath keeps in the bath temperature (37 ± 0.5) ℃, feeds 60-80 bubble/min of gaseous mixture, specimen load 1g, chart speed 4mm/min, range 20mv, balance 1h, write down one section normal contraction curve, adding final concentration is above-mentioned each drug solution of 1.0mg/ml, and the shrinkage amplitude and the contraction frequency of the interior uterine smooth muscle of 10min are recorded in it on desk-top balance recorder after the observed and recorded administration.
The influence of table 4 pair Mouse Uterus spontaneous activity
| Group | Dosage/mgml -1 | Shrinkage amplitude (g) | Contraction frequency (inferior/10min) | Contraction movement power (amplitude * frequency) |
| 2 groups of samples of 1 group of sample of matched group Radix Angelicae Sinensis polysaccharide Rhizoma Corydalis total alkaloids sample are 3 groups before the administration | - 1.0 1.0 1.0 1.0 1.0 | 1.06±0.11 0.75±0.14 * 0.85±0.19 ** 0.55±0.17 *** 0.51±0.25 *** 0.59±0.26 *** | 14.8±3.10 7.3±2.14 * 6.7±0.68 ** 6.1±1.11 ** 5.2±1.31 *** 6.4±0.91 *** | 15.69±2.45 5.48±2.13 ** 5.70±2.34 ** 3.36±1.83 *** 2.65±1.43 *** 3.78±1.80 ** |
Annotate: compare with matched group before the administration:: *: P＜0.05, * *: P＜0.01, * * *: P＜0.001
The experimental result of table 4 shows that the laboratory sample group of the present invention of two kinds of active component different proportion forms all can significantly suppress the uterine contraction amplitude.The result of table 3 also demonstrates, the laboratory sample of three groups of different proportion forms all can obviously suppress uterotonic amplitude, and sample 1, sample 2, sample 3 uterotonic inhibitory action all is better than the Radix Angelicae Sinensis polysaccharide group and the Rhizoma Corydalis total alkaloids group of single component, illustrate that pharmaceutical composition of the present invention has obvious synergistic function.
Following embodiment all can realize the described effect of above-mentioned experimental example.
Embodiment 1: the preparation of capsule
The preparation of Radix Angelicae Sinensis polysaccharide: Radix Angelicae Sinensis is with the distilled water reflux, extract, of 8 times of weight portions 3 times, and each 4h is after 3 times extracting solution merges, distilling under reduced pressure concentrates, and after the concentrated solution pre-cooling, adds the ethanol of 3 times of parts by volume, stir, after leaving standstill, slightly carried polysaccharide, slightly carried the polysaccharide dissolved in distilled water with the High speed refrigerated centrifuge collecting precipitation, under 4 ℃ of conditions to distill water dialysis 3 days, centrifugal removal precipitation, the supernatant lyophilization, promptly.
The preparation of Rhizoma Corydalis total alkaloids: get the Rhizoma Corydalis powder and put in the extractor, moistening with strong aqua ammonia, 6 times of parts by volume chloroform extraction 3 hours filter, the filtrate water bath method, promptly.
Radix Angelicae Sinensis polysaccharide 48g Rhizoma Corydalis total alkaloids 12g, get above-mentioned two flavor crude drug mix homogeneously after, add conventional adjuvant, use 70% alcohol granulation in the usual way, drying, encapsulated 1000, promptly.
Embodiment 2: the preparation of tablet
Radix Angelicae Sinensis polysaccharide 30kg Rhizoma Corydalis total alkaloids 30kg, get above-mentioned two flavor crude drug mix homogeneously after, add conventional adjuvant, use 70% alcohol granulation in the usual way, drying adds 0.5kg magnesium stearate mixing, compacting in flakes, promptly.
Embodiment 3: the preparation of granule
Radix Angelicae Sinensis polysaccharide 12kg Rhizoma Corydalis total alkaloids 48kg, get above-mentioned two flavor crude drug mix homogeneously after, add conventional adjuvant, use 70% alcohol granulation in the usual way, drying is made granule, promptly.
Embodiment 4: the preparation of injection
Radix Angelicae Sinensis polysaccharide 10g Rhizoma Corydalis total alkaloids 10g gets above-mentioned two flavor crude drug, with the dissolving of 200ml water for injection, adds 0.1% active carbon and stirs 5 minutes, filters, and water for injection adds to 1000ml, regulates pH value to 5.0, filter, and fill, sterilization, promptly.
Embodiment 5: the preparation of mixture
The preparation of Radix Angelicae Sinensis polysaccharide: Radix Angelicae Sinensis is with the distilled water reflux, extract, of 8 times of weight portions 3 times, and each 4h is after 3 times extracting solution merges, distilling under reduced pressure concentrates, and after the concentrated solution pre-cooling, adds the ethanol of 3 times of parts by volume, stir, after leaving standstill, slightly carried polysaccharide, slightly carried the polysaccharide dissolved in distilled water with the High speed refrigerated centrifuge collecting precipitation, under 4 ℃ of conditions to distill water dialysis 3 days, centrifugal removal precipitation, the supernatant lyophilization, promptly.
The preparation of Rhizoma Corydalis total alkaloids: get the Rhizoma Corydalis powder and put in the extractor, moistening with strong aqua ammonia, 6 times of parts by volume chloroform extraction 3 hours filter, the filtrate water bath method, promptly.
Get Radix Angelicae Sinensis polysaccharide 30g Rhizoma Corydalis total alkaloids 30g, get above-mentioned two flavor crude drug, after the pure water dissolving, add conventional adjuvant, add pure water, filter to full dose 10L, fill, mixture is made in sterilization.
Claims (11)
1, a kind of pharmaceutical composition for the treatment of dysmenorrhea is characterized in that the crude drug of this pharmaceutical composition consists of: Radix Angelicae Sinensis polysaccharide 1-9 weight portion Rhizoma Corydalis total alkaloids 1-9 weight portion; Wherein Radix Angelicae Sinensis polysaccharide adopts the phenolsulfuric acid method to measure, content with glucose meter greater than 50%; Rhizoma Corydalis total alkaloids adopts determined by ultraviolet spectrophotometry, content in tetrahydropalmatine greater than 50%.
2, pharmaceutical composition as claimed in claim 1 is characterized in that the crude drug of this pharmaceutical composition consists of: Radix Angelicae Sinensis polysaccharide 2 weight portion Rhizoma Corydalis total alkaloidss 8 weight portions.
3, pharmaceutical composition as claimed in claim 1 is characterized in that the crude drug of this pharmaceutical composition consists of: Radix Angelicae Sinensis polysaccharide 5 weight portion Rhizoma Corydalis total alkaloidss 5 weight portions.
4, pharmaceutical composition as claimed in claim 1 is characterized in that the crude drug of this pharmaceutical composition consists of: Radix Angelicae Sinensis polysaccharide 8 weight portion Rhizoma Corydalis total alkaloidss 2 weight portions.
5, as claim 1,2,3 or 4 described pharmaceutical compositions, it is characterized in that Radix Angelicae Sinensis polysaccharide is made by following method in the crude drug of this pharmaceutical composition: Radix Angelicae Sinensis is with the distilled water reflux, extract, of 6-10 times of weight portion 2-4 time, each 3-5h, after 2-4 time extracting solution merges, distilling under reduced pressure concentrates, after the concentrated solution pre-cooling, the ethanol that adds 2-4 times of parts by volume stirs, after leaving standstill, slightly carried polysaccharide with the High speed refrigerated centrifuge collecting precipitation, slightly carry the polysaccharide dissolved in distilled water, to distill water dialysis 2-4 days, centrifugal removal precipitated under the 3-5 ℃ of condition, the supernatant lyophilization, promptly; Rhizoma Corydalis total alkaloids is made by following method in the crude drug: get the Rhizoma Corydalis powder and put in the extractor, moistening with strong aqua ammonia, 5-7 times of parts by volume chloroform extraction 2-4 hour filters, the filtrate water bath method, promptly.
6, pharmaceutical composition as claimed in claim 5, it is characterized in that Radix Angelicae Sinensis polysaccharide is made by following method in the crude drug of this pharmaceutical composition: Radix Angelicae Sinensis is with the distilled water reflux, extract, of 8 times of weight portions 3 times, each 4h, after 3 times extracting solution merges, distilling under reduced pressure concentrates, after the concentrated solution pre-cooling, the ethanol that adds 3 times of parts by volume stirs, after leaving standstill, slightly carried polysaccharide with the High speed refrigerated centrifuge collecting precipitation, slightly carry the polysaccharide dissolved in distilled water, under 4 ℃ of conditions to distill water dialysis 3 days, centrifugal removal precipitation, the supernatant lyophilization, promptly; Rhizoma Corydalis total alkaloids is made by following method in the crude drug: get the Rhizoma Corydalis powder and put in the extractor, moistening with strong aqua ammonia, 6 times of parts by volume chloroform extraction 3 hours filter, the filtrate water bath method, promptly.
7, as claim 1,2,3,4 or 6 described pharmaceutical compositions, it is characterized in that getting pharmaceutical composition crude drug of the present invention, add conventional adjuvant, according to common process, make oral liquid formulations, syrup, mixture, capsule, pill, granule, tablet, intramuscular dose or intravenous injection.
8, preparation of drug combination method as claimed in claim 7, the preparation method that it is characterized in that ejection preparation is: get pharmaceutical composition crude drug of the present invention, with the dissolving of 75-200 parts by volume water for injection, add the 0.05-0.2% active carbon and stirred 3-7 minute, filter, water for injection adds to the 450-1000 parts by volume, regulate pH value to 4.0-6.0, filter fill, sterilization, promptly.
9, preparation of drug combination method as claimed in claim 8, the preparation method that it is characterized in that ejection preparation is: get pharmaceutical composition crude drug of the present invention, with the dissolving of 100 parts by volume waters for injection, add 0.1% active carbon and stirred 5 minutes, filter, water for injection adds to 500 parts by volume, regulate pH value to 5.0, filter fill, sterilization, promptly.
10, as the application of pharmaceutical composition as described in the claim 1,2,3 or 4 in preparation treatment dysmenorrhea medicine.
11, pharmaceutical composition as claimed in claim 5, it is characterized in that getting pharmaceutical composition crude drug of the present invention, add conventional adjuvant,, make oral liquid formulations, syrup, mixture, capsule, pill, granule, tablet, intramuscular dose or intravenous injection according to common process.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100789713A CN100502886C (en) | 2006-04-28 | 2006-04-28 | Medicinal composition for treating dysmenorrhea and preparation process thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100789713A CN100502886C (en) | 2006-04-28 | 2006-04-28 | Medicinal composition for treating dysmenorrhea and preparation process thereof |
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| Publication Number | Publication Date |
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| CN101062109A true CN101062109A (en) | 2007-10-31 |
| CN100502886C CN100502886C (en) | 2009-06-24 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108420855A (en) * | 2018-06-06 | 2018-08-21 | 四川省中医药科学院 | A kind of pharmaceutical composition of prevention dysmenorrhoea |
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2006
- 2006-04-28 CN CNB2006100789713A patent/CN100502886C/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108420855A (en) * | 2018-06-06 | 2018-08-21 | 四川省中医药科学院 | A kind of pharmaceutical composition of prevention dysmenorrhoea |
| CN108420855B (en) * | 2018-06-06 | 2020-11-17 | 四川省中医药科学院 | Pharmaceutical composition for preventing and treating dysmenorrhea |
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| CN100502886C (en) | 2009-06-24 |
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