CN101045169A - Process for preparing superthin allogeneic bone nail - Google Patents

Abstract

A process for preparing the superfine allogenic bone nail includes such steps as choosing allogenic cortical bone piece (8-15mmX5-8mmX3-7mm), removing periosteum and marrow, storing at -50--86 deg.C for 30-35 days, recovering ordinary temp, longitudinal cutting grinding to become bone nail (0.8-1.5mmX0.4-1.0mm), flushing with physiologic saline, immersing it in absolute alcohol for 2 hr and then in the mixture of anhydrous ether and acetone for 1 hr, natural drying, freeze-drying at -30--55 deg.C for 24 hr, vacuum packing, and gamma-ray sterilizing.

Description

The preparation technology of superthin allogeneic bone nail

Technical field

The present invention relates to a kind of preparation technology of nail, specifically, relate to a kind of preparation technology of allogeneic bone nail of extra small diameter.

Background technology

The annual fracture of ossiculum patient who causes because of fracture such as little joint, metacarpal-phalangeals is a lot.At present, the traditional therapy of this position fracture is the peg that has hammered into fixation at fracture, treat the bone healing after, again patient is carried out operation for the second time and will hammer into peg taking-up in the bone.The defective of this Therapeutic Method is that the operation second time of taking out peg has increased patient's misery greatly, and peg causes infection, inflammation and anaphylaxis easily in human body.

A kind of allogeneic bone nail is arranged, adopt the cortical bone material to make, also can be used for fixing fracture affected part, have and to be replaced by host bone, do not need advantages such as second operation taking-up.But the allogeneic bone nail of having developed, diameter all more than 3mm, are not suitable for the internal fixation treatment of fracture of ossiculum.

Summary of the invention

Technical problem to be solved by this invention provides a kind of preparation technology of superthin allogeneic bone nail, is applicable to the internal fixation treatment of fracture of ossiculum.

Technical scheme of the present invention is as follows:

A kind of preparation technology of superthin allogeneic bone nail comprises the steps:

(1) choose the allogeneic cortical bone fragment of long 8～15mm, wide 5～8mm, high 3～7mm, remove periosteum and bone marrow after, preserved 30～35 days down at-50 ℃～-86 ℃, take out back rewarming 1～1.5h;

(2) the vertical shape cutting of the cortical bone fragment after will handling is the bar column, uses the grinding machine reconditioning to become screw thread being arranged or not having the screw thread nail of diameter 0.8～1.5mm, length 0.4～1.0mm;

(3) with the preparation nail wash repeatedly with normal saline after, be immersed in 2h in the dehydrated alcohol, put into the mixed liquor 1h of absolute ether and acetone then, natural air drying;

(4) nail was placed in-30 ℃～-55 ℃ drying machines lyophilizing 24 hours;

(5) nail after the taking-up lyophilizing is loaded on vacuum seal in the packaging bag, after the gamma-ray irradiation sterilization, stores for future use under the room temperature.

Through mechanical test, by the superthin allogeneic bone nail of the present invention's preparation, its mechanical strength can satisfy the fixedly requirement of fracture of ossiculum, and has excellent biological compatibility and degradability, thereby has exempted the misery that patient's secondary is got tack operation.

The specific embodiment

Embodiment 1

Choose long 15mm, wide 5mm, the allogeneic cortical bone fragment of high 3mm, after removing periosteum and bone marrow, preserved 30 days down at-86 ℃, take out back rewarming 1.5h, its vertical shape cutting is the bar column, use the grinding machine reconditioning to become diameter 0.8mm, length 0.4mm has a screw thread nail, after washing repeatedly with normal saline, be immersed in 2h in the dehydrated alcohol, put into the mixed liquor 1h of absolute ether and acetone then, natural air drying, then place the interior lyophilizing of-30 ℃ of drying machines 24 hours, be loaded on vacuum seal in the packaging bag after the taking-up, after the gamma-ray irradiation sterilization, store for future use under the room temperature.The nail of making is carried out mechanical test, and its mechanics parameter is as follows: resistance to compression ultimate load 150.03MPa, anti-folding ultimate load 16.21MPa, tension ultimate load 110.95MPa, antitorque ultimate load 101.4MPa.

Claims (1)

1. the preparation technology of a superthin allogeneic bone nail comprises the steps:

(1) choose the allogeneic cortical bone fragment of long 8～15mm, wide 5～8mm, high 3～7mm, remove periosteum and bone marrow after, preserved 30～35 days down at-50 ℃～-86 ℃, take out back rewarming 1～1.5h;

(2) the vertical shape cutting of the cortical bone fragment after will handling is the bar column, uses the grinding machine reconditioning to become screw thread being arranged or not having the screw thread nail of diameter 0.8～1.5mm, length 0.4～1.0mm;

(3) with the preparation nail wash repeatedly with normal saline after, be immersed in 2h in the dehydrated alcohol, put into the mixed liquor 1h of absolute ether and acetone then, natural air drying;

(4) nail was placed in-30 ℃～-55 ℃ drying machines lyophilizing 24 hours;

(5) nail after the taking-up lyophilizing is loaded on vacuum seal in the packaging bag, after the gamma-ray irradiation sterilization, stores for future use under the room temperature.