CN101041839A - 用工程菌生产5-氨基乙酰丙酸的方法 - Google Patents
用工程菌生产5-氨基乙酰丙酸的方法 Download PDFInfo
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- CN101041839A CN101041839A CN 200710068169 CN200710068169A CN101041839A CN 101041839 A CN101041839 A CN 101041839A CN 200710068169 CN200710068169 CN 200710068169 CN 200710068169 A CN200710068169 A CN 200710068169A CN 101041839 A CN101041839 A CN 101041839A
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- 238000004519 manufacturing process Methods 0.000 title description 4
- 238000000034 method Methods 0.000 claims abstract description 12
- ZGXJTSGNIOSYLO-UHFFFAOYSA-N 88755TAZ87 Chemical compound NCC(=O)CCC(O)=O ZGXJTSGNIOSYLO-UHFFFAOYSA-N 0.000 claims abstract description 7
- 238000000855 fermentation Methods 0.000 claims abstract description 7
- 230000004151 fermentation Effects 0.000 claims abstract description 7
- 239000001963 growth medium Substances 0.000 claims abstract description 7
- 101150112623 hemA gene Proteins 0.000 claims abstract description 6
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 12
- 239000002609 medium Substances 0.000 claims description 12
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 239000004471 Glycine Substances 0.000 claims description 6
- 239000002253 acid Substances 0.000 claims description 6
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- 239000008103 glucose Substances 0.000 claims description 5
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- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 claims description 3
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 3
- 239000003112 inhibitor Substances 0.000 claims description 3
- 229940049547 paraxin Drugs 0.000 claims description 3
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- 241000193830 Bacillus <bacterium> Species 0.000 claims description 2
- 210000001072 colon Anatomy 0.000 claims description 2
- 241000588724 Escherichia coli Species 0.000 claims 1
- 229940023064 escherichia coli Drugs 0.000 claims 1
- 229960002749 aminolevulinic acid Drugs 0.000 abstract description 2
- 238000010899 nucleation Methods 0.000 abstract 3
- 238000012258 culturing Methods 0.000 abstract 2
- 239000000463 material Substances 0.000 abstract 1
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- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
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- 241000588722 Escherichia Species 0.000 description 1
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- 201000010989 colorectal carcinoma Diseases 0.000 description 1
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- 208000021045 exocrine pancreatic carcinoma Diseases 0.000 description 1
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- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
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Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2007100681690A CN100572546C (zh) | 2007-04-20 | 2007-04-20 | 用工程菌生产5-氨基乙酰丙酸的方法 |
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CNB2007100681690A CN100572546C (zh) | 2007-04-20 | 2007-04-20 | 用工程菌生产5-氨基乙酰丙酸的方法 |
Publications (2)
Publication Number | Publication Date |
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CN101041839A true CN101041839A (zh) | 2007-09-26 |
CN100572546C CN100572546C (zh) | 2009-12-23 |
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CNB2007100681690A Expired - Fee Related CN100572546C (zh) | 2007-04-20 | 2007-04-20 | 用工程菌生产5-氨基乙酰丙酸的方法 |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102747114A (zh) * | 2012-01-17 | 2012-10-24 | 浙江大学 | 一种用短暂厌氧发酵调节重组大肠杆菌代谢的方法 |
CN104561158A (zh) * | 2015-01-13 | 2015-04-29 | 江南大学 | 一种添加Fe2+提高大肠杆菌工程菌合成5-氨基乙酰丙酸的方法 |
CN103981203B (zh) * | 2013-02-07 | 2018-01-12 | 中国科学院天津工业生物技术研究所 | 5‑氨基乙酰丙酸高产菌株及其制备方法和应用 |
CN114381416A (zh) * | 2022-03-23 | 2022-04-22 | 北京道合成企业管理有限公司 | 一种高产5-氨基乙酰丙酸的重组大肠杆菌菌株及其应用 |
-
2007
- 2007-04-20 CN CNB2007100681690A patent/CN100572546C/zh not_active Expired - Fee Related
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102747114A (zh) * | 2012-01-17 | 2012-10-24 | 浙江大学 | 一种用短暂厌氧发酵调节重组大肠杆菌代谢的方法 |
CN102747114B (zh) * | 2012-01-17 | 2014-09-03 | 浙江大学 | 一种用短暂厌氧发酵调节重组大肠杆菌代谢的方法 |
CN103981203B (zh) * | 2013-02-07 | 2018-01-12 | 中国科学院天津工业生物技术研究所 | 5‑氨基乙酰丙酸高产菌株及其制备方法和应用 |
CN104561158A (zh) * | 2015-01-13 | 2015-04-29 | 江南大学 | 一种添加Fe2+提高大肠杆菌工程菌合成5-氨基乙酰丙酸的方法 |
CN104561158B (zh) * | 2015-01-13 | 2018-01-16 | 江南大学 | 一种添加Fe2+提高大肠杆菌工程菌合成5‑氨基乙酰丙酸的方法 |
CN114381416A (zh) * | 2022-03-23 | 2022-04-22 | 北京道合成企业管理有限公司 | 一种高产5-氨基乙酰丙酸的重组大肠杆菌菌株及其应用 |
CN114381416B (zh) * | 2022-03-23 | 2022-06-28 | 北京道合成企业管理有限公司 | 一种高产5-氨基乙酰丙酸的重组大肠杆菌菌株及其应用 |
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Publication number | Publication date |
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CN100572546C (zh) | 2009-12-23 |
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Assignee: Suzhou Yian Biotech Co., Ltd. Assignor: Zhejiang University Contract record no.: 2010320000856 Denomination of invention: Method for producing 5-glycyl ethylformic acid by using engineering bacterium Granted publication date: 20091223 License type: Exclusive License Open date: 20070926 Record date: 20100705 |
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