CN101028314A - Liquid coating against tumor, virus and bacterium - Google Patents
Liquid coating against tumor, virus and bacterium Download PDFInfo
- Publication number
- CN101028314A CN101028314A CN 200610045985 CN200610045985A CN101028314A CN 101028314 A CN101028314 A CN 101028314A CN 200610045985 CN200610045985 CN 200610045985 CN 200610045985 A CN200610045985 A CN 200610045985A CN 101028314 A CN101028314 A CN 101028314A
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- virus
- liquid
- bacterium
- liquid coating
- against tumor
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- Medicines Containing Plant Substances (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
An antineoplastic, antiviral and antibacterial liquid dressing for suppressing tumor cells, adenovirus, parainfluenza virus, syncytial virus, Coxsackie virus and bacteria contains brucea fruit oil (or liquid) (95-99 Wt portions) and clove oil (or liquid) (1-5).
Description
Technical field:
The present invention relates to a kind of antitumor, virus, bacterial liquid dressing.
Background technology:
At present, can antitumor, again can antiviral, the medicine of antibacterial belongs to blank.
Summary of the invention:
The technical problem to be solved in the present invention provide a kind of can antitumor, again can antiviral, the liquid dressing of antibacterial.Technical scheme: a kind of antitumor, virus, bacterial liquid dressing is characterized in that: contained composition and shared parts by weight are Fel Anas domestica seed oil or liquid 95-99, Oleum Caryophylli or liquid 1-5.
Beneficial effect: Fel Anas domestica seed oil or liquid can play the effect of antitumor, virus, and Oleum Caryophylli or liquid can play the effect of antiviral, antibacterial.
Further specify beneficial effect of the present invention below in conjunction with test.
Suppress the experiment of tumor cell
SGC-7901 makes 4 * 10 with cancerous cell
4Cell/ml suspension, add in 96 well culture plates, every hole 100 μ l, wherein stay 3 holes to add 10% hyclone culture fluid, 100 μ l, as blank, put in the saturated water-bath incubator of 37 degree 5% carbon dioxide and cultivated 24 hours, make it adherent, every hole adds different dilution dressing 0.1ml, and each dilution factor adds three holes, cell matched group and the every hole of blank group add culture fluid 100 μ l respectively, put in the saturated water-bath incubator of 37 degree 5% carbon dioxide and cultivated 48 hours, finish cultivation and added buffer 100 μ l and MTT20 μ l in preceding 4 hours, continue to cultivate, discard culture fluid after cultivating end, every hole adds 100 μ lDMSO and shakes up, and reads 490nm wavelength absorbance (OD490) on microplate reader, calculates the meansigma methods of each concentration, compare with matched group, calculate inhibitory rate of cell growth.
Result such as following table:
| Extension rate of the present invention (X) | Suppression ratio (%) |
| 256 | 62.2 |
| 512 | 62.1 |
| 1024 | 52.4 |
| 2048 | 48.3 |
| 4096 | 39.9 |
| 8192 | 38.9 |
By table as seen, the present invention has very strong inhibition tumor cell effect, when diluting 2000 times, to the suppression ratio of tumor cell still near 50%.
Antibacterial tests
(1) extracorporeal bacteria inhibitor test: adopting test tube doubly to measure dilution method, is culture medium with the ordinary broth, and 22 kinds of strains such as dysentery bacillus, staphylococcus aureus, Bacillus typhi have been carried out extracorporeal bacteria inhibitor test research, and the result shows; The water decoction of a, Folium Caryophylli is to common pyogenic infection staphylococcus aureus and Staphylococcus albus, to causing the bacillus typhi murium of alimentary toxicosis, Salmonellas etc. have tangible antibacterial action b, to the staphylococcus aureus and the various dysentery bacterium of the amine of anti-the semi-annular jade pendant, the leaf of Syringa oblata Lindl. water decoction has tangible bacteriostasis.
(2) bacteriostatic test in the body; Mice infected such as staphylococcus aureus, dysentery bacterium in the body had significant protective effect, ED
50Be 4.1-5.9g/kg.
Antivirus action
Adenovirus (Afv3, Afv7), parainfluenza virus (I, II, III), respiratory syncytial virus (RSV) and Coxsackie virus (CoxB1, CoxB2, CoxB3, CoxB4, CoxB5, CoxB6) institute cytopathogenic effect are had obvious inhibitory action, and its potency is better than Radix Isatidis granules.
The specific embodiment:
Preparation the present invention can pass through the carbon dioxide abstraction supercritical respectively, obtains Fel Anas domestica seed oil and Oleum Caryophylli, selects 96 parts of Fructus Bruceae weight of oil umbers, and the Oleum Caryophylli parts by weight mix for 4 parts, removes moisture, promptly gets the present invention.All right, the method by decocting obtains Fructus Bruceae liquid and lilac liquid respectively, selects 97 parts of Fructus Bruceae liquid parts by weight, and the lilac liquid parts by weight mix for 3 parts, promptly get the present invention.During use, the each external application 2-5 of the present invention milliliter.
Claims (1)
1, a kind of antitumor, virus, bacterial liquid liquid dressing is characterized in that: contained composition and shared parts by weight are Fel Anas domestica seed oil or liquid 95-99, Oleum Caryophylli or liquid 1-5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610045985 CN101028314A (en) | 2006-03-04 | 2006-03-04 | Liquid coating against tumor, virus and bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200610045985 CN101028314A (en) | 2006-03-04 | 2006-03-04 | Liquid coating against tumor, virus and bacterium |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101028314A true CN101028314A (en) | 2007-09-05 |
Family
ID=38713965
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200610045985 Pending CN101028314A (en) | 2006-03-04 | 2006-03-04 | Liquid coating against tumor, virus and bacterium |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101028314A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102697858A (en) * | 2012-06-08 | 2012-10-03 | 东北农业大学 | Application of clove leaves or extract thereof in preparation of swine influenza virus resistant medicament |
| CN103405646A (en) * | 2013-08-04 | 2013-11-27 | 张珍平 | TCM composition for treating recurrent-attack senile vaginitis |
| CN104056299A (en) * | 2014-07-08 | 2014-09-24 | 烟台隽秀生物科技有限公司 | Liquid wound protecting membrane and preparation method thereof |
| US11451677B2 (en) | 2018-09-25 | 2022-09-20 | Fujifilm Business Innovation Corp. | Control device and non-transitory computer readable medium storing control program |
-
2006
- 2006-03-04 CN CN 200610045985 patent/CN101028314A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102697858A (en) * | 2012-06-08 | 2012-10-03 | 东北农业大学 | Application of clove leaves or extract thereof in preparation of swine influenza virus resistant medicament |
| CN102697858B (en) * | 2012-06-08 | 2014-09-17 | 东北农业大学 | Application of clove leaves or extract thereof in preparation of swine influenza virus resistant medicament |
| CN103405646A (en) * | 2013-08-04 | 2013-11-27 | 张珍平 | TCM composition for treating recurrent-attack senile vaginitis |
| CN104056299A (en) * | 2014-07-08 | 2014-09-24 | 烟台隽秀生物科技有限公司 | Liquid wound protecting membrane and preparation method thereof |
| US11451677B2 (en) | 2018-09-25 | 2022-09-20 | Fujifilm Business Innovation Corp. | Control device and non-transitory computer readable medium storing control program |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |