CN101020722B - Process of extracting soluble polysaccharide from fragile blueberry leaf - Google Patents
Process of extracting soluble polysaccharide from fragile blueberry leaf Download PDFInfo
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- CN101020722B CN101020722B CN2007100676531A CN200710067653A CN101020722B CN 101020722 B CN101020722 B CN 101020722B CN 2007100676531 A CN2007100676531 A CN 2007100676531A CN 200710067653 A CN200710067653 A CN 200710067653A CN 101020722 B CN101020722 B CN 101020722B
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Abstract
The process of extracting soluble polysaccharide from fragile blueberry leaf includes the following steps: 1. drying fragile blueberry leaf and crushing in a crusher; 2. leaching fragile blueberry leaf powder in hot distilled water, cooling and filtering to obtain filtrate; 3. centrifuging the filtrate to obtain liquid phase and precipitate; 4. washing the liquid phase through mixing with 80 % concentration alcohol solution and centrifuging to obtain fragile blueberry polysaccharide; 5. washing the liquid phase through mixing with 80 % concentration alcohol solution and centrifuging to obtain fragile blueberry polysaccharide repeatedly for 1-3 times; and 6. vacuum drying the collected fragile blueberry polysaccharide. The present invention has simple technological process and high extracting rate.
Description
Technical field
The present invention relates to the extracting method of soluble polysaccharide, particularly a kind of method of from the Ericaceae myrtillin leave, extracting soluble polysaccharide.
Background technology
Fructus Vaccinii Bracteati is an Ericaceae genus vaccinium plant, it is very wide to distribute in China, and standing stock are very big, main product in East China, ground such as Central China, south China, often be distributed in the hillside woods of hilly country or height above sea level 400～1400m or in the thicket, be the indicative plant of sour earth (red soil).It is reported that it has memory, antifatigue and cold-resistant function etc., and myopia, senile cataract, diabetes etc. are had improvement and preventive effect.Biological activitys such as that many studies show that, vegetable polysaccharides have is antitumor, hypoglycemic, reducing blood-fat and anticoagulation, biological activity such as antitumor, hypoglycemic, reducing blood-fat of Fructus Vaccinii Bracteati polysaccharide then awaits further research.Study on nutrition report to Fructus Vaccinii Bracteati or congener is more, but the Study on extraction of Fructus Vaccinii Bracteati polysaccharide be there is no report both at home and abroad.
Summary of the invention
The technical problem to be solved in the present invention provides the method for extracting soluble polysaccharide from Fructus Vaccinii Bracteati that a kind of technology is simple, extraction yield is high.
In order to solve the problems of the technologies described above, the invention provides a kind of method of from myrtillin leave, extracting soluble polysaccharide, may further comprise the steps:
1), drying and crushing: after fresh myrtillin leave cleans up and dries, put 40～60 ℃ of drying in oven and pulverize with the plant pulverizer after 24～36 hours, the myrtillin leave powder after the pulverizing can be crossed 120 mesh sieves;
2), lixiviate: after above-mentioned myrtillin leave powder and distilled water mixed by 1: 5～1: 20 weight ratio, hot dipping was carried 1～5 hour in 30 ℃～100 ℃ hot water baths, cooled and filtered, filtrate;
3), centrifugal: above-mentioned filtrate is centrifugal, obtain liquid phase I and the precipitation I;
4), washing: with liquid phase I and concentration is that 80% alcohol mixes, stirs with 1: 5～1: 10 weight ratio, must the Fructus Vaccinii Bracteati polysaccharide after centrifugal;
5), secondary washing: with the washings and the concentration of above-mentioned centrifugal back gained is that 80% alcohol mixes, stirs with 1: 5～1: 10 weight ratio, must the Fructus Vaccinii Bracteati polysaccharide after centrifugal;
6), repeated washing: repeating step 5) 1～3 time;
7), drying: collect step 4)～6) the Fructus Vaccinii Bracteati polysaccharide of gained carries out vacuum-drying.
As improvement of extracting the method for soluble polysaccharide from myrtillin leave of the present invention: the centrifugal condition in step 3), step 4) and the step 5) is: in the centrifugal 20～30min of 2900～3100r/min.
The method of from myrtillin leave, extracting soluble polysaccharide of the present invention, the rotating speed of plant pulverizer is 1400r/min in the step 1), the pulverizing time is 25～35min.
The method of extracting soluble polysaccharide from Fructus Vaccinii Bracteati of the present invention, technology is simple, extraction yield is high.Being convenient to follow-up scholar studies the two-way function of " soluble polysaccharide " composition in the Fructus Vaccinii Bracteati.The secondary washing of being carried out in the method for the present invention and the purpose of repeated washing are: in order fully to extract the Fructus Vaccinii Bracteati polysaccharide, improve its extraction yield.
Embodiment
Embodiment 1, a kind of method of from myrtillin leave, extracting soluble polysaccharide, carry out following steps successively:
1), drying and crushing: after fresh myrtillin leave 100g cleans up and dries, put to dry by the fire in 50 ℃ of baking ovens after 24 dried hours and pulverize with the plant pulverizer, plant pulverizer rotating speed is 1400r/min, and the pulverizing time is 25min, and the powder after the pulverizing can be crossed 120 mesh sieves; Get myrtillin leave powder 13.9g.
2), lixiviate: after above-mentioned myrtillin leave powder and distilled water mixed by 1: 5 weight ratio, hot dipping was carried 1 hour in 30 ℃ of hot water baths, is cooled to the room temperature after-filtration; Get filtrate;
3), centrifugal: that above-mentioned filtrate in the centrifugal 20min of 3000r/min, is obtained liquid phase I and precipitation I;
4), washing: with liquid phase I and concentration is that 80% alcohol mixes, stirs with 1: 5 weight ratio, is separated into 2 parts behind the centrifugal 20min of 3000r/min: washings and Fructus Vaccinii Bracteati polysaccharide;
5), secondary washing: with the washings of above-mentioned centrifugal back gained and concentration is that 80% alcohol mixes, stirs with 1: 5 weight ratio, is separated into 2 parts after centrifugal once more: washings and Fructus Vaccinii Bracteati polysaccharide;
6), repeated washing: repeating step 5) 3 times;
7), drying: collection step 4)～6) the Fructus Vaccinii Bracteati polysaccharide of gained carries out vacuum-drying, gets Fructus Vaccinii Bracteati polysaccharide 0.1556g.
Embodiment 2, a kind of method of from myrtillin leave, extracting soluble polysaccharide, carry out following steps successively:
1), drying and crushing: after fresh myrtillin leave 100g cleans up and dries, put to dry by the fire after 36 hours in 60 ℃ of baking ovens and pulverize with the plant pulverizer, plant pulverizer rotating speed is 1400r/min, and the pulverizing time is 35min, powder after the pulverizing can be crossed 120 mesh sieves, gets myrtillin leave powder 14.1g.
2), lixiviate: after above-mentioned myrtillin leave powder and distilled water mixed by 1: 10 weight ratio, hot dipping was carried 3 hours in 100 ℃ of hot water baths, is cooled to the room temperature after-filtration; Get filtrate;
3), centrifugal: as, to obtain liquid phase I and precipitation I with the centrifugal 30min of above-mentioned filtrate 3100r/min;
4), washing: with liquid phase I and concentration is that 80% alcohol mixes, stirs with 1: 10 weight ratio, is separated into 2 parts behind the centrifugal 20min of 3100r/min: washings and Fructus Vaccinii Bracteati polysaccharide;
5), secondary washing: with the washings of above-mentioned centrifugal back gained and concentration is that 80% alcohol mixes, stirs with 1: 10 weight ratio, is separated into 2 parts once more behind the centrifugal 20min of 3100r/min: washings and Fructus Vaccinii Bracteati polysaccharide;
6), repeated washing: repeating step 5) 2 times;
7), drying: collection step 4)～6) the Fructus Vaccinii Bracteati polysaccharide of gained carries out vacuum-drying, gets the Fructus Vaccinii Bracteati polysaccharide and promptly gets Fructus Vaccinii Bracteati polysaccharide 0.243g.
Embodiment 3, a kind of method of from myrtillin leave, extracting soluble polysaccharide, carry out following steps successively:
1), drying and crushing: after fresh myrtillin leave 100g cleans up and dries, put to dry by the fire after 30 hours in 55 ℃ of baking ovens and pulverize with the plant pulverizer, plant pulverizer rotating speed is 1400r/min, and the pulverizing time is 30min, powder after the pulverizing can be crossed 120 mesh sieves, gets myrtillin leave powder 14.0g.
2), lixiviate: after above-mentioned myrtillin leave powder and distilled water mixed by 1: 20 weight ratio, hot dipping was carried 5 hours in 65 ℃ of hot water baths, is cooled to the room temperature after-filtration; Get filtrate;
3), centrifugal: that above-mentioned filtrate in the centrifugal 25min of 2900r/min, is obtained liquid phase I and precipitation I;
4), washing: with liquid phase I and concentration is that 80% alcohol mixes, stirs with 1: 8 weight ratio, is separated into 2 parts behind the centrifugal 20min of 2900r/min: washings and Fructus Vaccinii Bracteati polysaccharide;
5), secondary washing: with the washings of above-mentioned centrifugal back gained is that 80% alcohol mixes, stirs with 1: 8 weight ratio with concentration once more, is separated into 2 parts behind the centrifugal 25min of 2900r/min once more: washings and Fructus Vaccinii Bracteati polysaccharide;
6), repeated washing: repeating step 5) 1 time;
7), drying: collection step 4)～6) the Fructus Vaccinii Bracteati polysaccharide of gained carries out vacuum-drying, promptly gets Fructus Vaccinii Bracteati polysaccharide 0.102g.
At last, it is also to be noted that what more than enumerate only is several specific embodiments of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (4)
1. method of extracting soluble polysaccharide from myrtillin leave is characterized in that may further comprise the steps:
1), drying and crushing: after fresh myrtillin leave cleans up and dries, put 40～60 ℃ of drying in oven and pulverize with the plant pulverizer after 24～36 hours, the myrtillin leave powder after the pulverizing can be crossed 120 mesh sieves;
2), lixiviate: after above-mentioned myrtillin leave powder and distilled water mixed by 1: 5～1: 20 weight ratio, hot dipping was carried 1～5 hour in 30 ℃～100 ℃ hot water baths, cooled and filtered, filtrate;
3), centrifugal: above-mentioned filtrate is centrifugal, obtain liquid phase I and the precipitation I;
4), washing: with liquid phase I and concentration is that 80% alcohol mixes, stirs with 1: 5～1: 10 weight ratio, must the Fructus Vaccinii Bracteati polysaccharide after centrifugal;
5), secondary washing: with the washings and the concentration of above-mentioned centrifugal back gained is that 80% alcohol mixes, stirs with 1: 5～1: 10 weight ratio, must the Fructus Vaccinii Bracteati polysaccharide after centrifugal;
6), repeated washing: repeating step 5) 1～3 time;
7), drying: collect step 4)～6) the Fructus Vaccinii Bracteati polysaccharide of gained carries out vacuum-drying.
2. the method for extracting soluble polysaccharide from myrtillin leave according to claim 1 is characterized in that: described step 3) is in the centrifugal 20～30min of 2900～3100r/min.
3. the method for extracting soluble polysaccharide from myrtillin leave according to claim 2 is characterized in that: described step 4) is in the centrifugal 20～30min of 2900～3100r/min.
4. the method for extracting soluble polysaccharide from myrtillin leave according to claim 3 is characterized in that: described step 5) is in the centrifugal 20～30min of 2900～3100r/min.
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| CN2007100676531A CN101020722B (en) | 2007-03-27 | 2007-03-27 | Process of extracting soluble polysaccharide from fragile blueberry leaf |
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| CN2007100676531A CN101020722B (en) | 2007-03-27 | 2007-03-27 | Process of extracting soluble polysaccharide from fragile blueberry leaf |
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| CN101020722B true CN101020722B (en) | 2010-09-08 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110386991A (en) * | 2018-04-17 | 2019-10-29 | 南京先华生物科技有限公司 | Fragile blueberry fruit polysaccharide extracting process and its application |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101726560B (en) * | 2008-10-10 | 2013-03-27 | 中国科学院沈阳应用生态研究所 | Method for measuring nutrient concentration of leaves |
| CN102429084B (en) * | 2011-11-29 | 2013-11-13 | 内蒙古伊利实业集团股份有限公司 | Oriental blueberry leaf extract-added cold beverage and preparation method thereof |
| CN102964464A (en) * | 2012-11-30 | 2013-03-13 | 江南大学 | Method for extracting and purifying bracteatum polysaccharide with blood sugar regulation activity |
| CN103113756B (en) * | 2013-03-06 | 2014-12-10 | 无锡市金和泰农业生物技术有限公司 | Method for efficiently extracting and preparing vaccinium leaves pigment |
| CN105777927A (en) * | 2016-04-20 | 2016-07-20 | 江苏长运生物科技有限公司 | Separation and purification method for Vaccinium bracteatum Thunb. leaf polysaccharide and blueberry leaf polysaccharide |
| CN114874348B (en) * | 2022-06-14 | 2023-02-03 | 常熟理工学院 | Vaccinium bracteatum thunb leaf polysaccharide VBLP-3b and preparation method and application thereof |
| CN114835830B (en) * | 2022-06-14 | 2023-02-03 | 常熟理工学院 | Blueberry leaf polysaccharide and preparation method and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1792179A (en) * | 2005-12-28 | 2006-06-28 | 金华职业技术学院 | Antistaling agent for fruits or vegetable, and its prepn. method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1792179A (en) * | 2005-12-28 | 2006-06-28 | 金华职业技术学院 | Antistaling agent for fruits or vegetable, and its prepn. method |
Non-Patent Citations (1)
| Title |
|---|
| 朱加进."苦菜中可溶性多糖提取工艺研究".《农业工程学报》第18卷 第1期.2002,第18卷(第1期),138-140. * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110386991A (en) * | 2018-04-17 | 2019-10-29 | 南京先华生物科技有限公司 | Fragile blueberry fruit polysaccharide extracting process and its application |
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