CN101016421A - Process of producing gardenia blue pigment - Google Patents
Process of producing gardenia blue pigment Download PDFInfo
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- CN101016421A CN101016421A CN 200710020158 CN200710020158A CN101016421A CN 101016421 A CN101016421 A CN 101016421A CN 200710020158 CN200710020158 CN 200710020158 CN 200710020158 A CN200710020158 A CN 200710020158A CN 101016421 A CN101016421 A CN 101016421A
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- blue pigment
- gardenia blue
- penetrating fluid
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- aerobic fermentation
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Abstract
The invention discloses a preparing craft method of cape jasmine blue pigment, which comprises the following steps: producing hybrid seeds with aspergillus niger bacterial; yeasting; separating zymotic fluid; presenting colour of osmosis liquid; spraying; drying; getting cape jasmine blue pigment solid powder. This invention adopt glutamic acid or leucine or mixture as colour reagent, which possesses simple craft and low cost.
Description
Technical field
The invention belongs to a kind of process for extracting of natural pigment, specifically is a kind of producing and manufacturing technique that extracts gardenia blue pigment from the jasminoidin mother liquor.
Background technology
Cape jasmine is a traditional Chinese medicine, first medicine-food two-purpose resource that also belongs to the promulgation of China Ministry of Health, have effects such as the liver of protecting, cholagogic, step-down, hemostasis, heat-clearing, sharp cold, antidiarrheal, on tcm clinical practice, be usually used in treating diseases such as icterohepatitis, sprain and contusion, digestive tract hemorrhage, hypertension, diabetes, the high heat of flu.Cape jasmine fruit mainly contains and is xanchromatic Crocin class, has the cape jasmine glycoside of significant curative effect and polyphenol etc.Can obtain through refining multiple pigment from the cape jasmine fruit, they are used for food color, not only color and luster can match in excellence or beauty with corresponding synthetic colour, but also has the curative effect effect.It is safe, nontoxic, have no side effect, it is functional natural colorant, after being in harmonious proportion separately or with the natural pigment of other reddish yellow strains, this pigment can produce the tone of a series of bluish-green variations, the fields such as food, medicine, makeup that can be applied to are used very extensive to remedy the deficiency of natural bluish-green pigment variety development.
The manufacturer of present domestic gardenia blue pigment seldom, output is not high yet.The production technique of gardenia blue can be divided into two big classes: one-step fermentation and two-step fermentation.One-step fermentation promptly is to finish the hydrolysis of jasminoidin and the colour developing of hydrolysate during the fermentation simultaneously; Two-step fermentation is exactly that the colour developing of the hydrolysate of jasminoidin was divided into for two steps, first hydrolysis, colour developing again.Again colour developing liquid is carried out separation and purification.Up to the present, the production of highly purified gardenia blue pigment still rests on the laboratory scale, and on the industrial production, the production technique of gardenia blue pigment does not all have too many deposition, and some research and development institutions can only produce the green gardenia blue of grey blanking bar.Generally, the big polychrome valency of the gardenia blue pigment of domestic production is low, cost is high, and production technique is not suitable for large-scale industrial production.Therefore, utilize modern separation method, explore the new manufacturing condition of gardenia blue pigment, obtain the low-cost gardenia blue pigment product of high luminance relay valency and will have good prospect.
By own practice is added in the deciphering of existing theoretical and technology, the applicant thinks, wants to generate blue pure lucid and lively gardenia blue, problem that must three aspects of solution: the making with extra care of (1) raw material.Mainly remove different jasminoidin, α Crocin, heterocyclic amino acid, polyphenol etc. and be unfavorable for generating the chemical ingredients of cyanine; (2) optimization of color reaction condition.Conditions such as leavening temperature, fermentation pH value, fermentation time, developer addition, developing time and colour temp are optimized, obtain the gardenia blue pigment of high luminance relay valency; (3) sfgd. behind the colour generation.How research controlled the factor that enzyme, remaining amino acid and the monose and the ultraviolet ray etc. of the back inactivation that ferments influences look valency stability, with the stability of assurance gardenia blue pigment.
Summary of the invention
The producing and manufacturing technique that the purpose of this invention is to provide a kind of gardenia blue pigment, use the method that novel fermentation technology, membrane separation technique and spray drying technology combine, and by optimizing processing parameter, effectively make with extra care raw material, reduced the substrate that hinders color reaction, the secretion of having regulated microbial enzyme system, production cost is low, productive rate is high, and product look valency is higher than domestic level.
This technology adopts two-step approach to produce gardenia blue pigment, promptly obtain the hydrolysate of jasminoidin earlier by microbial fermentation, again hydrolysate and amino acid are carried out color reaction generation gardenia blue pigment, utilize membrane sepn to carry out the gardenia blue pulvis that purifying obtains the high luminance relay valency at last.
Technical scheme of the present invention is as follows:
The producing and manufacturing technique of gardenia blue pigment is characterized in that may further comprise the steps:
(1), the production of hybrid seeds: aspergillus niger strain slant culture, preparation spore suspension;
(2), fermentation: spore suspension and jasminoidin mother liquor are added aerobic fermentation in the fermention medium;
(3), separate: fermented liquid is carried out after the coarse filtration again through ultra-filtration membrane separated and collected penetrating fluid, make that the molecular weight of effective constituent reaches below 800 in the penetrating fluid;
(4), colour generation: the 0.8%-1.8% massfraction of pressing penetrating fluid adds L-glutamic acid or leucine or its mixture as developer, and 75-83 ℃ is incubated 4-6 hour down; PH value is controlled between the 6.5-8.0.
(5), spraying drying: colour generation liquid concentrates the back spraying drying and obtains the gardenia blue pigment pressed powder.
The component of fermention medium is in described (2) step: wheat bran 1--2%; Rice bran 1.5--3%; (NH
4)
2SO
40.2%; KH
2PO
40.1%; MgSO
47H
2O0.05%; CaCl
20.2%, all the other are water; When aerobic fermentation began, the jasminoidin final concentration was 3--4% in the fermented liquid; Aerobic fermentation condition: 30--34 ℃, 42-48 hour aerobic fermentation.
In described (4) step, the 0.8%-1.2% massfraction of pressing penetrating fluid adds L-glutamic acid or leucine or its any mixture as developer, and 80 ℃ are incubated 5 hours down; PH value is controlled between the 6.5-7.5.
The gordian technique explanation
The prescription of fermention medium and fermentation parameter:
Substratum is one of three elements of zymotechnique, also is the vital strategic secrets of zymotechnique, and each component has very large influence to the quality of final product in the substratum.The substratum of this technology and fermentation parameter can improve the hydrolysis rate of jasminoidin and the colour generation of minimal interference gardenia blue to greatest extent, help improving the quality of products when reducing production costs, and bring considerable economic.
Membrane sepn concentrates
Membrane separation technique is the relevant screening process of a kind of and membrane pore size size, pressure difference with the film both sides is a motivating force, with the film is filtration medium, under certain pressure, when stoste flow through the film surface, the many tiny micropore that gathers in the film surface only allowed water and small-molecule substance by becoming through liquid, and volume then is trapped within the liquid feeding side of film in the stoste greater than the material in film surface micropore footpath, become concentrated solution, thereby realize separation and spissated purpose stoste.
Different according to the character such as molecular weight, apparent molecular weight and molecular structure of jasminoidin hydrolysate and other impurity reach separation to target product, concentrate by changing membrane pore size, mould material, membrane operations parameter etc.By the feed liquid purity height that film is handled, content can reach more than 20%.Membrane sepn is applied on the extractive technique of jasminoidin hydrolysate, significant advantage is arranged at aspects such as energy efficient, raising quality, optimization technologies.
The color reaction condition
Color reaction is the committed step of whole technology, has directly determined the quality of the finished product.Comprised four elements in the color reaction: amino acid kind, temperature of reaction, reaction times and pH value.
The jasminoidin hydrolyzate presents a series of different color reactions with different amino acid in conjunction with meeting, as sapphirine, faint blue, blue-greenish colour even red-purple.Temperature of reaction, reaction times and pH value are to the color and luster and the stable influence that also has in various degree of product.Therefore, have only by a large amount of orthogonal tests and could finally obtain the suitable color reaction condition of the present invention.
Advantage of the present invention:
Production technique is simple, and cost is low, productive rate is high, product look valency height.
Description of drawings
Fig. 1 is a process flow sheet of the present invention.
Embodiment
Referring to accompanying drawing.
The technological process of production of the present invention and processing parameter are as follows:
1. fermention medium:
Wheat bran 1--2%; Rice bran 1.5--3%; (NH
4)
2SO
40.2%; KH
2PO
40.1%; MgSO
47H
2O 0.05%; CaCl
20.2%.
2. bacterial classification: aspergillus niger
The seed preparation: starting strain inoculation PDA inclined-plane, put 28 ℃ of cultivation 4d and treat that spore covers with.Making spore suspension with sterilized water during inoculation inserts in the fermention medium.
3. jasminoidin is handled: with water-soluble 30% the mother liquor of making of jasminoidin pulvis, degerming adds in the fermention medium to final concentration 3--4% before the fermentation beginning.
4. fermentation condition: 30--34 ℃, the 48h aerobic fermentation.
5. membrane sepn concentrates: after fermented liquid is carried out coarse filtration and handles, concentrate through micro-filtration, two-stage ultrafiltering and three grades of membrane sepn of reverse osmosis, make that the molecular weight of effective constituent reaches below 800 in the penetrating fluid;
6. colour generation: 1% massfraction of pressing penetrating fluid adds L-glutamic acid or leucine as developer, 80 ℃ of insulation 5h.
7. spraying drying: inlet temperature is 160 ℃.
Table 1 gardenia blue product physical and chemical index
| Project | Company standard | This product quality indicator |
| The maximum absorption wave max that grows into, nm | 595-605nm | 595-605nm |
| Look valency: E 1cm 1％595nm≥ | 60 | 70 |
| Water content: %≤ | 20.0 | 10 |
| Ash content :≤ | 1.0％ | 0.5％ |
| Arsenic (in As) mg/kg :≤ | 2 | 2 |
| Heavy metal (in Pb) mg/kg :≤ | 30 | 30 |
| Plumbous Pb mg/kg :≤ | 0.5 | 0.5 |
Table 2 gardenia blue product microbiological indicator
| Project | Company standard |
| Total number of bacterial colony/(cfu/g)≤ | 1000 |
| Coliform/(MPN/100g)≤ | 30 |
| Pathogenic bacterium (pathogen enterobacteria and pathogenic coccus) | Must not detect |
| Mold count/(cfu/g)≤ | 50 |
Claims (3)
1, the producing and manufacturing technique of gardenia blue pigment is characterized in that may further comprise the steps:
(1), the production of hybrid seeds: aspergillus niger strain slant culture, preparation spore suspension;
(2), fermentation: spore suspension and jasminoidin mother liquor are added aerobic fermentation in the fermention medium;
(3), separate: fermented liquid is carried out after the coarse filtration again through ultra-filtration membrane separated and collected penetrating fluid, make that the molecular weight of effective constituent reaches below 800 in the penetrating fluid;
(4), colour generation: the 0.8%-1.8% massfraction of pressing penetrating fluid adds L-glutamic acid or leucine or its mixture as developer, and 75-83 ℃ is incubated 4-6 hour down; PH value is controlled between the 6.5-8.0.
(5), spraying drying: colour generation liquid concentrates the back spraying drying and obtains the gardenia blue pigment pressed powder.
2, the producing and manufacturing technique of gardenia blue pigment according to claim 1 is characterized in that the component of fermention medium in described (2) step is: wheat bran 1--2%; Rice bran 1.5--3%; (NH
4)
2SO
40.2%; KH
2PO
40.1%; MgSO
47H
2O 0.05%; CaCl
20.2%, all the other are water; When aerobic fermentation began, the jasminoidin final concentration was 3--4% in the fermented liquid; Aerobic fermentation condition: 30--34 ℃, 42-48 hour aerobic fermentation.
3, the producing and manufacturing technique of gardenia blue pigment according to claim 1, it is characterized in that in described (4) step, the 0.8%-1.2% massfraction of pressing penetrating fluid adds L-glutamic acid or leucine or its any mixture as developer, and 80 ℃ are incubated 5 hours down; PH value is controlled between the 6.5-7.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100201585A CN100516143C (en) | 2007-02-16 | 2007-02-16 | Process of producing gardenia blue pigment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2007100201585A CN100516143C (en) | 2007-02-16 | 2007-02-16 | Process of producing gardenia blue pigment |
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| Publication Number | Publication Date |
|---|---|
| CN101016421A true CN101016421A (en) | 2007-08-15 |
| CN100516143C CN100516143C (en) | 2009-07-22 |
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ID=38725647
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2007100201585A Expired - Fee Related CN100516143C (en) | 2007-02-16 | 2007-02-16 | Process of producing gardenia blue pigment |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101177541B (en) * | 2007-12-12 | 2010-05-19 | 福建农林大学 | Method for preparing alcohol-soluble gardenia blue pigment derivatives |
| CN103232724A (en) * | 2013-03-31 | 2013-08-07 | 新疆刀郎枣业有限公司 | Aspergillus niger liquid fermentation assisted method for extraction of red date pigment |
| US20130202703A1 (en) * | 2010-09-27 | 2013-08-08 | Shin Sadano | Method for producing gardenia blue pigment |
| CN103265824A (en) * | 2013-06-08 | 2013-08-28 | 湖北工业大学 | Production and refining method of gardenia red pigment |
| CN104745636A (en) * | 2014-11-27 | 2015-07-01 | 安徽农业大学 | Preparation method of gardenia blue pigment |
-
2007
- 2007-02-16 CN CNB2007100201585A patent/CN100516143C/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101177541B (en) * | 2007-12-12 | 2010-05-19 | 福建农林大学 | Method for preparing alcohol-soluble gardenia blue pigment derivatives |
| US20130202703A1 (en) * | 2010-09-27 | 2013-08-08 | Shin Sadano | Method for producing gardenia blue pigment |
| CN103232724A (en) * | 2013-03-31 | 2013-08-07 | 新疆刀郎枣业有限公司 | Aspergillus niger liquid fermentation assisted method for extraction of red date pigment |
| CN103265824A (en) * | 2013-06-08 | 2013-08-28 | 湖北工业大学 | Production and refining method of gardenia red pigment |
| CN103265824B (en) * | 2013-06-08 | 2014-06-18 | 湖北工业大学 | Production and refining method of gardenia red pigment |
| CN104745636A (en) * | 2014-11-27 | 2015-07-01 | 安徽农业大学 | Preparation method of gardenia blue pigment |
| CN104745636B (en) * | 2014-11-27 | 2018-02-09 | 安徽农业大学 | The preparation method of gardenia blue pigment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN100516143C (en) | 2009-07-22 |
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Assignee: Anhui Yaqiang Bioengineering Co., Ltd. Assignor: Hefei University of Technology Contract fulfillment period: 2009.8.10 to 2016.8.10 contract change Contract record no.: 2009340000160 Denomination of invention: Process of producing gardenia blue pigment Granted publication date: 20090722 License type: Exclusive license Record date: 2009.8.11 |
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Free format text: EXCLUSIVE LICENSE; TIME LIMIT OF IMPLEMENTING CONTACT: 2009.8.10 TO 2016.8.10; CHANGE OF CONTRACT Name of requester: ANHUI YAQIANG BIOENGINEERING CO., LTD. Effective date: 20090811 |
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Granted publication date: 20090722 Termination date: 20120216 |