CN101006823A - Preparation method of apple seeds protein - Google Patents
Preparation method of apple seeds protein Download PDFInfo
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- CN101006823A CN101006823A CN 200710017246 CN200710017246A CN101006823A CN 101006823 A CN101006823 A CN 101006823A CN 200710017246 CN200710017246 CN 200710017246 CN 200710017246 A CN200710017246 A CN 200710017246A CN 101006823 A CN101006823 A CN 101006823A
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- seeds protein
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Abstract
The invention relates to a method for preparing protein by using residues of materials after fat extraction, especially the vegetables and fruit seed with high protein content, especially to a method for preparing apple seeds protein. Apple seeds has high content of oil and has a protein content of 46.0%. Thus apple seeds have high exploitation value. There is no report about the preparation method of apple seeds protein in our country and abroad. The steps of the method are: apple seeds residue obtain from hyper-criticality CO2 flow extraction-crushing-extracting with lye-whizzing-stagnating with acid-whizzing-spray-drying to obtain apple seeds protein. The method is suitable for the extraction of apple seeds protein. Apple seeds protein can be extracted efficiently by the method of the invention at a yield of 50.41%.
Description
Technical field:
The present invention relates to the residue after the extraction of grease-contained material process grease is carried out the preparation of albumen, to containing the high fruits and vegetables seed of protein, is a kind of preparation method of apple seeds protein specifically especially.
Background technology
China has abundant apple resource, and at present, nearly 3,000 ten thousand mu of China's apple cultivation gross area is produced apple per year and reached 2,200 ten thousand tons, accounts for the nearly 50% of Gross World Product, is the first in the world big producing country.Because the distinctive resources advantage of China, fruit juice processing industry development in recent years is rapid, and production capacity is increased sharply to present more than 40 ten thousand tons by the not enough kiloton of 20th century the mid-80.The apple that be used to process national every year has 6,000,000 tons approximately, drains about 2,000,000 tons of pomace, the apple seed that every year can be about 2.5 ten thousand tons.
Vegetable protein has more own exclusive characteristic than animal protein, and it does not contain cholesterol, has water-retaining property and shape-retaining ability in process, no longer produces variations such as contraction behind mechanical-moulded, heating and regulating, and product property is even, helps prolonging storage life.The apple seed except that oil content than (24.2%) higher, its protein content has the higher development value also up to 46.0%.
In food industry was produced, the comprehensive utilization of resource was one of important directions of research both at home and abroad.China's food resource comprehensive utilization ratio is one of minimum several countries in the whole world, and the discarded object in many processing is actually the raw material that important other products is produced, as the seed resource in the fruit processing.The apple seed is a discarded object of producing products such as cider, dried apple slices, canned apple.With the discarded object in the apple processing---isolated apple seed is a raw material the pomace (being the process from production of fodder or extraction pectin or production apple dietary fiber generally speaking), utilize the food separation extractive technique, prepare apple seeds protein the residue after extracting grease, realize the comprehensive utilization of resource.
Therefore the apple seed has higher development value, and the production that can be nutraceutical provides valuable base-material.
At present, the preparation method of apple seeds protein does not appear in the newspapers both at home and abroad.
Summary of the invention
The object of the present invention is to provide the preparation method of the high apple seeds protein of a kind of preparation method who is applicable to apple seeds protein and yield.
For achieving the above object, the technical solution used in the present invention is:
A kind of preparation method of apple seeds protein is characterized in that: described apple seeds protein preparation method's step is as follows:
Learnt from else's experience apple seed residue behind the supercritical carbon dioxide extraction (acquisition apple seed oil), by pulverizing (140 order), alkali lye extracts (optimum extraction process condition: pH:11.0, temperature: 50 ℃, time: 40min, solid-liquid ratio (g/ml): 1: 40, extract 2 times, in leaching process, slowly stir), centrifugation (rotating speed 6000r/min, centrifugation time 10min), acid is heavy [to add saturated citric acid solution slowly in leaching liquor, regulate pH to 4.0,4 ℃ leave standstill 60min], (the rotating speed 6000r/min of centrifugation again, centrifugation time 10min), [the sour hypostasis that centrifugation is obtained adds water in the ratio of 1: 2 (g/ml) to spray-drying, and reconciles pH to 7.0, constantly is being heated to 60 ℃ under the condition of stirring, 180 ℃ of EATs, carry out spray-drying under 70 ℃ of the air outlet temperature] obtain apple seeds protein.
Adopt the present invention to obtain apple seeds protein effectively, the yield that makes apple seeds protein is up to 50.41%.
The specific embodiment
The method that the present invention extracts apple seeds protein is: sink → centrifugation → spray-drying → apple seeds protein through the apple seed residue → pulverizing → alkali lye extraction → centrifugation → acid behind the supercritical carbon dioxide extraction (acquisition apple seed oil), this method is applicable to that apple seeds protein extracts; Important technical parameters is in the method for the present invention: material therefor is through the apple seed residue behind the supercritical carbon dioxide extraction (acquisition apple seed oil); Grinding particle size 140 orders of material; The condition that alkali lye extracts is pH:11.0, temperature: 50 ℃, and time: 40min, solid-liquid ratio (g/ml): 1: 40, extract 2 times, in leaching process, slowly stir; The heavy condition of acid is regulated pH to 4.0 for add saturated citric acid solution slowly in the leaching liquor that centrifugation obtains, and 4 ℃ leave standstill 60min; [the sour hypostasis that centrifugation is obtained adds water in the ratio of 1: 2 (g/ml) to spray-drying, and reconciles pH to 7.0, constantly is being heated to 60 ℃ under the condition of stirring, and 180 ℃ of EATs, the air outlet temperature is carried out spray-drying for 70 ℃.The yield of apple seeds protein is 50.41% with this understanding.
Specific embodiment:
Apple seed residue 1Kg behind the supercritical carbon dioxide extraction (acquisition apple seed oil) learnt from else's experience, by pulverizing (140 order), pack in the extraction vessel, add water 40L, adjusting the pH value with NaOH under slow condition of stirring is 11.0, and be heated to 50 ℃ of constant temperature and pick up counting, behind 40min, stop to stir, leave standstill 20min, remove the impurity of suspension, carry out 2 times according to said process and extract, extract obtained under the condition of 6000r/min centrifugation 10min, in the leaching liquor that centrifugation obtains, add saturated citric acid solution slowly, regulate pH to 4.0,4 ℃ leave standstill 60min, remove supernatant, centrifugation 10min under the condition of 6000r/min, the sour hypostasis that centrifugation is obtained adds 2L water, adjusts pH to 7.0 with NaOH, constantly be heated to 60 ℃ under the condition of stirring, 180 ℃ of EATs, carry out spray-drying under 70 ℃ of the air outlet temperature, obtain apple seeds protein 0.5041Kg.
Claims (6)
1, the preparation method of apple seeds protein is characterized in that: described apple seeds protein preparation method's step is as follows:
The supercritical carbon dioxide extraction of learning from else's experience, apple seed residue behind the acquisition apple seed oil, pulverize 140 orders, extract 2 times through alkali lye, after in leaching process, slowly stirring,, again centrifugation heavy through centrifugation, acid, sour hypostasis that centrifugation is obtained are in 1: the ratio of 2g/ml adds water, and reconcile pH to 7.0, constantly carrying out spray-drying under the condition of stirring, obtain apple seeds protein.
2, the preparation method of apple seeds protein according to claim 1 is characterized in that: the raw material of described apple seeds protein preparation is process supercritical carbon dioxide extraction, the apple seed residue behind the acquisition apple seed oil.
3, the preparation method of apple seeds protein according to claim 1 is characterized in that: described alkali lye extracts optimum process condition: pH:11.0, temperature: 50 ℃, time: 40min, solid-liquid ratio: 1: 40g/ml extracts 2 times, slowly stirs in leaching process.
4, the preparation method of apple seeds protein according to claim 1 is characterized in that: the heavy condition of described best acid is: constantly slowly adding saturated citric acid solution under the condition of stirring, regulating pH to 4.0,4 ℃ leave standstill 60min.
5, the preparation method of apple seeds protein according to claim 1 is characterized in that: described best spray-drying condition is: material is reconciled pH to 7.0 and is preheated to 60 ℃, 180 ℃ of EATs, 70 ℃ of air outlet temperature.
6, the preparation method of apple seeds protein according to claim 1 is characterized in that: described twice centrifugation, each rotating speed 6000r/min, centrifugation time 10min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710017246A CN101006823B (en) | 2007-01-18 | 2007-01-18 | Preparation method of apple seeds protein |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710017246A CN101006823B (en) | 2007-01-18 | 2007-01-18 | Preparation method of apple seeds protein |
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| Publication Number | Publication Date |
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| CN101006823A true CN101006823A (en) | 2007-08-01 |
| CN101006823B CN101006823B (en) | 2010-05-26 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN200710017246A Expired - Fee Related CN101006823B (en) | 2007-01-18 | 2007-01-18 | Preparation method of apple seeds protein |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589760B (en) * | 2008-05-29 | 2012-02-29 | 中国人民解放军总后勤部军需装备研究所 | Industrial hempseeds isolated protein powder and preparation method thereof |
| CN105076746A (en) * | 2015-07-03 | 2015-11-25 | 池州清源钓鱼用品有限责任公司 | Delicious soft-shelled turtle feed and preparation method thereof |
| CN105104771A (en) * | 2015-07-03 | 2015-12-02 | 池州清源钓鱼用品有限责任公司 | Rapid trionyx sinensis fattening feed and preparation method |
| CN105124174A (en) * | 2015-07-03 | 2015-12-09 | 池州清源钓鱼用品有限责任公司 | Turtle forage improving meat quality and preparation method thereof |
-
2007
- 2007-01-18 CN CN200710017246A patent/CN101006823B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101589760B (en) * | 2008-05-29 | 2012-02-29 | 中国人民解放军总后勤部军需装备研究所 | Industrial hempseeds isolated protein powder and preparation method thereof |
| CN105076746A (en) * | 2015-07-03 | 2015-11-25 | 池州清源钓鱼用品有限责任公司 | Delicious soft-shelled turtle feed and preparation method thereof |
| CN105104771A (en) * | 2015-07-03 | 2015-12-02 | 池州清源钓鱼用品有限责任公司 | Rapid trionyx sinensis fattening feed and preparation method |
| CN105124174A (en) * | 2015-07-03 | 2015-12-09 | 池州清源钓鱼用品有限责任公司 | Turtle forage improving meat quality and preparation method thereof |
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| Publication number | Publication date |
|---|---|
| CN101006823B (en) | 2010-05-26 |
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