CN100590431C - Method for manufacturing minisize capillary pipe electrophoresis electrochemistry detecting pool - Google Patents
Method for manufacturing minisize capillary pipe electrophoresis electrochemistry detecting pool Download PDFInfo
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- CN100590431C CN100590431C CN200710030381A CN200710030381A CN100590431C CN 100590431 C CN100590431 C CN 100590431C CN 200710030381 A CN200710030381 A CN 200710030381A CN 200710030381 A CN200710030381 A CN 200710030381A CN 100590431 C CN100590431 C CN 100590431C
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- capillary pipe
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Abstract
The invention designs a capillary electrophoresis electrochemical test pool made of poly-dimethyl siloxane (PDMS); PDMS is a silicon rubber with good light transmittance and low refractive index; using PDMS to make test pool has the following advantages: simple manufacturing process, good demoulding effect and high yield rate.
Description
Technical field
The present invention relates to the electrochemistry detecting apparatus field, a kind of minisize capillary pipe electrophoresis electrochemistry detecting pool and preparation method thereof of saying so more specifically.
Technical background
In Capillary Electrophoresis, Electrochemical Detection is a class sensitivity, simple, inexpensive method, and once being called is the most potential a kind of detection technique in CE future.Electrochemical Detection is that the electrochemical properties according to electrochemical principle and material detects.Though electrochemical detector has the distinct advantages that its mass detection limit is low, the range of linearity is wide, selectivity is good, instrument is simple and cheap, but still is not widely used.Trace it to its cause, one of them where the shoe pinches is the preparation of ultramicroelectrode detection cell.
At present all types of method of bibliographical information adopts on capillary wall punching to insert electrode to detect mostly, and these class methods need relatively harsher process conditions usually, and difficulty reaches, and exists electrode to change and clean shortcomings such as difficulty; How microelectrode being fixed with capillary outlet and aimed in styletable detects also is a problem demanding prompt solution.
The macromolecular material cost is low, and handling ease is suitable for making disposable device.Thereby the research of making integrated capillary electrophoretic chip with superpolymer has in recent years caused people's interest.PDMS (Poly (dimethylsiloxane) claims the silicone high-elasticity body again) chemistry dimethyl silicone polymer by name is a kind of silicon rubber, has that light transmission is good, refractive index is low, an insulation and can characteristics such as produce in enormous quantities by the die casting method.
Summary of the invention
The objective of the invention is to overcome the shortcoming of prior art, provide a kind of simple in structure, moulding and be convenient in demoulding the minisize capillary pipe electrophoresis electrochemistry detecting pool that to produce in batches and preparation method thereof.
The present invention is achieved through the following technical solutions its purpose.
The present invention has designed a kind of employing dimethyl silicone polymer and has made minisize capillary pipe electrophoresis electrochemistry detecting pool, and PDMS is a kind of silicon rubber, has that light transmission is good, refractive index is low, an insulation and can characteristics such as produce in enormous quantities by the die casting method.Make detection cell with PDMS, manufacture craft is very simple, and stripping result is good, and the yield rate height has the characteristics of production in enormous quantities.
The structure of minisize capillary pipe electrophoresis electrochemistry detecting pool is for being provided with detection cell in the middle of the body of pond, the pond body sidewall offers the pipeline that is used to install separation capillary, detecting electrode and contrast electrode, and pipeline communicates with detection cell.Further structural improvement be the pipeline that is used to separation capillary and detecting electrode are installed at same straight line, make things convenient for separation capillary to export butt joint like this in detecting electrode.
The present invention also provides a kind of method for making of minisize capillary pipe electrophoresis electrochemistry detecting pool, may further comprise the steps:
1. on the box-like mould side wall, punch, insert diameter stainless steel wire between 300 to 450 microns, be made into required microchannel layout;
2. in dimethyl silicone polymer: the ratio of hardening agent is dimethyl silicone polymer and hardening agent to be mixed in 10: 1, carries out vacuumize degassing 20 minutes;
3. the dimethyl silicone polymer after will outgasing waters and casts from the box-like mould, and the thickness of casting exceeds stainless steel wire, heat-treats 0.5 hour under 60 ℃ temperature;
4. cool off, the demoulding;
5. extract stainless steel wire out, and in the middle of the body of pond, cut out detection cell.
As seen by above-mentioned, this miniature detection cell is compared with traditional detection cell, have more attractive in appearance, advantage such as volume is little, and is easy to carry, the damping fluid consumption is few, main is that its method for making is very easy, just can make in common lab.Can also be designed to the detection cell of various different passages according to the different needs of each experiment.Can directly regulate the position of capillary outlet and detecting electrode, significantly reduce the volume of self assembly instrument, instrument is developed to microminiaturized direction.
Description of drawings
Fig. 1 is a structural representation of the present invention;
Fig. 2 is the front view of Fig. 1;
Fig. 3 leads the testing result synoptic diagram for electricity of the present invention;
Fig. 4 is an Amperometric Detection Coupled result schematic diagram of the present invention.
Embodiment
The present invention is described further below in conjunction with accompanying drawing.
As shown in Figure 1, the pond body 1 of minisize capillary pipe electrophoresis electrochemistry detecting pool is cube structure, offer columniform detection cell 2 in the centre of one face, internal diameter is 0.5cm, on its side, offer four pipelines 31,32,33 and 34 that communicate with detection cell 2, be respectively applied for separation capillary 4, detecting electrode 5, contrast electrode 6 and negative pole 7 are installed.Again as shown in Figure 2, the pipeline 31 and 32 that is used to separation capillary 4 and detecting electrode 5 are installed helps separation capillary 4 like this and exports in the butt joint of detecting electrode 5 at same straight line.
Detailed manufacturing process of the present invention is as follows:
At first be the manufacturing process of mould, with stainless steel box water, alcohol wash is dried, or dries up with hair-dryer, and the about 380 microns stainless steel wire of external diameter is inserted in the correct position punching around can, is made into required microchannel layout.
Be to carry out PDMS prepolymer preparation then, in Sylgard184 type PDMS matrix: the ratio of hardening agent is that 10: 1 (mass ratio) takes by weighing an amount of PDMS matrix and hardening agent, mixes vacuumize degassing 20min.
Be cast in process that heating is shaped and be the PDMS after the degassing carefully is cast in the mould of the good stainless steel wire of cloth, the prepolymer thickness of cast should slightly exceed stainless steel, it is transferred to thermal treatment 0.5h in 60 ℃ the baking oven again.
To the miniature electrochemical investigating pond of casting cool off, the demoulding.Take out the mould that is shaped from baking oven, after the cooling, stainless steel wire is carefully extracted out, form pipeline, slough mould, cut out the detection cell at center, obtain the PDMS detection cell.Pipeline can insert separation capillary and detecting electrode and contrast electrode, and makes things convenient for separation capillary to export butt joint in detecting electrode.
The result that electricity of the present invention is led detection as shown in Figure 3.
Operating conditions in Amperometric Detection Coupled is: separation capillary-50 μ m * 36cm, separation voltage-15kV, the trishydroxymethylaminomethane of buffer solution-10mmol/L-1mmol/L citric acid.The result as shown in Figure 4, DA is a dopamine, NP is an adrenaline.
In sum, minisize capillary pipe electrophoresis electrochemistry detecting pool of the present invention is compared with traditional detection cell, Have more attractive in appearance, the advantage such as volume is little, and is easy to carry, the buffer solution consumption is few, main is its making side Method is very easy, just can make in common lab. Can also be designed to according to the different needs of each experiment The detection cell of various different passages. Can directly regulate the position of capillary outlet and detecting electrode, greatly reduce The volume of self assembly instrument, make instrument to microminiaturized future development.
Claims (4)
1. the method for making of a minisize capillary pipe electrophoresis electrochemistry detecting pool is characterized in that may further comprise the steps:
1. on the box-like mould side wall, punch, insert stainless steel wire, be made into required microchannel layout;
2. in dimethyl silicone polymer: the ratio of hardening agent is dimethyl silicone polymer and hardening agent to be mixed in 10: 1, and outgases;
3. the dimethyl silicone polymer after will outgasing waters and casts from the box-like mould, and the thickness of casting exceeds stainless steel wire, dries;
4. cool off, the demoulding;
5. extract stainless steel wire out, and in the middle of the body of pond, cut out detection cell.
2. according to the method for making of right 1 described minisize capillary pipe electrophoresis electrochemistry detecting pool, the diameter that it is characterized in that described stainless steel wire is between 300 to 450 microns.
3. according to the method for making of right 1 described minisize capillary pipe electrophoresis electrochemistry detecting pool, it is characterized in that the process of the degassing was to carry out vacuumize degassing 20 minutes during step 2..
4. according to the method for making of right 1 described minisize capillary pipe electrophoresis electrochemistry detecting pool, it is characterized in that the process of oven dry was to heat-treat 0.5 hour during step 3. under 60 ℃ temperature.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710030381A CN100590431C (en) | 2007-09-21 | 2007-09-21 | Method for manufacturing minisize capillary pipe electrophoresis electrochemistry detecting pool |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200710030381A CN100590431C (en) | 2007-09-21 | 2007-09-21 | Method for manufacturing minisize capillary pipe electrophoresis electrochemistry detecting pool |
Publications (2)
| Publication Number | Publication Date |
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| CN101135663A CN101135663A (en) | 2008-03-05 |
| CN100590431C true CN100590431C (en) | 2010-02-17 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN200710030381A Expired - Fee Related CN100590431C (en) | 2007-09-21 | 2007-09-21 | Method for manufacturing minisize capillary pipe electrophoresis electrochemistry detecting pool |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102507709A (en) * | 2011-11-01 | 2012-06-20 | 广东药学院 | Manufacture method for electrochemical detection device for microchip capillary electrophoresis |
| CN113413934A (en) * | 2021-07-16 | 2021-09-21 | 安徽骆华生物科技有限公司 | Preparation method and application of cross flow focusing type droplet micro-fluidic chip |
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2007
- 2007-09-21 CN CN200710030381A patent/CN100590431C/en not_active Expired - Fee Related
Non-Patent Citations (2)
| Title |
|---|
| 毛细管电泳－柱端电化学检测池的设计. 吴性良等.色谱，第17卷第2期. 1999 |
| 毛细管电泳－柱端电化学检测池的设计. 吴性良等.色谱，第17卷第2期. 1999 * |
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| CN101135663A (en) | 2008-03-05 |
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Granted publication date: 20100217 Termination date: 20110921 |