CN100587450C - Freezing Ballantine smear inspection method - Google Patents
Freezing Ballantine smear inspection method Download PDFInfo
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- CN100587450C CN100587450C CN200510034324A CN200510034324A CN100587450C CN 100587450 C CN100587450 C CN 100587450C CN 200510034324 A CN200510034324 A CN 200510034324A CN 200510034324 A CN200510034324 A CN 200510034324A CN 100587450 C CN100587450 C CN 100587450C
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- cell
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- freezing
- smear
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Abstract
A method for detecting Pasteur smear of frozen type includes using sampler to collect cell sample from neck of uterus and coating collected cell sample on clean glass plate, placing said glass plate in fixing liquid of aceto-alcohol and taking glass plate out after cell sample is fixed, soaking said glass plate on cell denaturation recovery liquid for 1-5 min., taking it out then placing it in freezer for refrigerating to make water content in cell sample be formed to be ice crystal, taking it out to carry out HE stain on it at normal temperature then making observation and diagnosis under microscope.
Description
Technical field
The present invention relates to a kind of freezing Ballantine smear inspection.
Background technology
At present, existing nearly 50 years of traditional Pap smear The Application of Technology is for extremely important contribution has been made in the control of cervical carcinoma in the world wide.The method that it adopts is to use sampling thiefs such as cotton swab and scraper plate to gather cell sample at the uterine neck place, directly smears in directly dyeing on the slide and after in time fixing.Though it is it is simple and efficient, with low cost.But this its still have a following shortcoming: 1), cell harvesting is not comprehensive, the smear cell overlap, the fixing back of applying high density ethanol or ethanol ether mixed stationary liquid cellular contraction is obvious, and mucinous degeneration solidifies, and coloring agent is difficult for penetrating the mucinous degeneration film in the dyeing course, cause the cell of mucus parcel painted bad, caryoplasm dyeing is not good enough, and especially smear is thicker, and the significant regional cell of cell overlap is differentiated very poor, have a strong impact on observation, easily cause and fail to pinpoint a disease in diagnosis mistaken diagnosis; 2), fail dyeing in time when fixing cell and handle then cell easy deformation, follow-up Color will be had a strong impact on.For this reason, though the medical expert has invented the various kinds of cell improving technology in succession, have certain effect for solving traditional Pap smear part defective, as technology such as TCT, LCT, LPT, but these technical operation complexity, need possess specific installation and special-purpose consumptive material, with high costs, be difficult to generally promote, have great limitation in the practical application, particularly when carrying out cell smear in enormous quantities, above-mentioned technological deficiency shows more outstandingly.
Summary of the invention
In order to solve above-mentioned the deficiencies in the prior art, the object of the present invention is to provide a kind of with low costly, cell is painted good, freezing Ballantine smear inspection simple to operate, practical.
Technical scheme of the present invention is: freezing Ballantine smear inspection may further comprise the steps:
A), gather cell sample at the uterine neck place with the cell sample device earlier;
B), then the cell sample of sampling thief collection is smeared on the slide of cleaning;
C), the more above-mentioned slide that smears cell sample was positioned in the ethanol acetate mixed stationary liquid of 0.1%-5% acetic acid 15 to 30 minutes, treat that cell sample on the slide is fixed after, take out;
D), the cell sample on the slide that step c is taken out is immersed in acetic acid cell degeneration between the 0.1%-10% and recovered in the liquid 1-5 minute, take out;
E), that the slide that again steps d taken out is put into freezer unit is freezing, makes the moisture content that infiltrates in the cell sample form ice crystal;
F), last, will take out through the slide of the band cell sample of freezing processing, directly carry out at normal temperatures being put in the microscopically inspections and examinations after HE dyeing or Pap smear dyeing, the dyeing.
It is freezing more than 30 minutes that described slide is put into freezer unit.
Described freezer unit is a refrigerator.
The invention has the beneficial effects as follows:
Freezing Ballantine smear inspection of the present invention, 1), because the slide through fixing band cell recovers the liquid processing through sex change, and the interior quick-frozen of (subzero) refrigerator at low temperatures, each composition perviousness of cell obviously strengthens, thereby through after the above-mentioned processing, slide with cell directly enters dyeing in the coloring agent, the painted remarkable improvement of cell, each composition of caryoplasm and cell is clear, boundary is clear between cell and the cell, differentiate preferablely, caryoplasm is painted clearly demarcated, has improved the not good state of traditional Pap smear cell dyeing significantly, even smear has certain overlapping, the wheel angle of cell is still clear and legible, is specially adapted to the repair process that long-time drying was kept somewhere back slide cell because Cell sheet glass fails in time to handle when woman in enormous quantities examined, to recovering the degenerating cell form and keeping good Color to play an important role; 2) use this technology to need not specific installation and consumptive material, it is with low cost, and is simple to operate, practical, is beneficial to all-round popularization, has very strong using value.
Embodiment
Freezing Ballantine smear inspection may further comprise the steps:
A), gather cell sample at the uterine neck place with the cell sample device earlier;
B), then the cell sample of sampling thief collection is smeared on the slide of cleaning;
C), the more above-mentioned slide that smears cell sample was positioned in the ethanol acetate mixed stationary liquid of 0.1%-5% acetic acid 15 to 30 minutes, treat that cell sample on the slide is fixed after, take out;
D), the cell sample on the slide that step c is taken out is immersed in acetic acid cell degeneration between the 0.1%-10% and recovered in the liquid 1-5 minute, take out;
E), that the slide that again steps d taken out is put into freezer unit is freezing, makes the moisture content that infiltrates in the cell sample form ice crystal;
F), last, will take out through the slide of the band cell sample of freezing processing, directly carry out at normal temperatures being put in the microscopically inspections and examinations after HE dyeing or Pap smear dyeing, the dyeing.
Immerse slide to put into freezer unit more freezing more than 30 minutes and recover liquid through cell degeneration.Described freezer unit is a refrigerator.It is the acetate solution of concentration between 0.1%-10% that described sex change recovers liquid, this liquid more easily penetrates in the mucus and cell membrane and nucleus of sex change, reach further fixing, strengthen the perviousness of mucus and cell, play form and promotion cell dyeing that freezing swelling property is cut out the effect of body and recovered cell, help the observation of pair cell form.
The principle of technology: mucus and cell after process ethanol acetate mixed stationary liquid is fixing, under the effect of these immobile liquids, play the good form when keeping cell to live, protein molecular generation structure in its mucus becomes, form the comparatively fine and close membranoid substance of one deck adhere to and be wrapped in cell around, the concentrated structure change of form synchronism also takes place in intracellular various compositions after the cell fixation, although play the effect of relative maintenance cellular morphology, but cell and cellular content shrink obviously, strand takes place and connects in the mucus molecule of distortion, be enclosed in densely cell around, through the Cell sheet glass of fixing Cell sheet glass of ethanol acetate mixed liquor or dry sex change after sex change recovers liquid and further handles, because sex change recovery liquid itself has stronger osmosis and enters rapidly in mucus and the cell, quick-frozen in (subzero) refrigerator at low temperatures, applying waterborne liquid is in the principle of ice body state lower volume mechanicalness expanse, infiltrate through mucus and intracellular liquid forms ice crystal under freezing state, in cell, expand and reach form and the volume of tension force when recovering cell life in limited time until cell membrane, immersing sex change in the mucus recovers behind the liquid under freezing state ice crystal and expands in having between the glycoprotein mucus molecule that to a certain degree sex change strand connects, cause strand to connect the molecule untwisted, various cells and cell component and mucus molecule spreading are between the ice crystal that recovers liquid formation, mucus becomes loose thin on the form, stick between mucus molecule and the cell and loosen, each composition perviousness of cell obviously strengthens.Through after the above-mentioned processing, slide with cell directly enters dyeing in the coloring agent, the painted remarkable improvement of cell, each composition of caryoplasm and cell is clear, boundary is clear between cell and the cell, differentiate preferable, caryoplasm is painted clearly demarcated, improved the not good state of traditional Pap smear cell dyeing significantly, even smear has certain overlapping, but the wheel angle of cell is still clear and legible, is specially adapted to the repair process that long-time drying was kept somewhere back slide cell because Cell sheet glass fails in time to handle when woman in enormous quantities examined, to recovering the degenerating cell form and keeping good Color to play an important role.
Claims (3)
1. freezing Ballantine smear inspection is characterized in that, may further comprise the steps:
A), gather cell sample at the uterine neck place with the cell sample device earlier;
B), then the cell sample of sampling thief collection is smeared on the slide of cleaning;
C), the more above-mentioned slide that smears cell sample was positioned in the ethanol acetate mixed stationary liquid of 0.1%-5% acetic acid 15 to 30 minutes, treat that cell sample on the slide is fixed after, take out;
D), the cell sample on the slide that step c is taken out is immersed in acetic acid cell degeneration between the 0.1%-10% and recovered in the liquid 1-5 minute, take out;
E), that the slide that again steps d taken out is put into freezer unit is freezing, makes the moisture content that infiltrates in the cell sample form ice crystal;
F), last, will take out through the slide of the band cell sample of freezing processing, directly carry out at normal temperatures being put in the microscopically inspections and examinations after HE dyeing or Pap smear dyeing, the dyeing.
2. according to the described freezing Ballantine smear inspection of claim 1, it is characterized in that it is freezing more than 30 minutes that described slide is put into freezer unit.
3. according to the described freezing Ballantine smear inspection of claim 1, it is characterized in that described freezer unit is a refrigerator.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200510034324A CN100587450C (en) | 2005-04-29 | 2005-04-29 | Freezing Ballantine smear inspection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200510034324A CN100587450C (en) | 2005-04-29 | 2005-04-29 | Freezing Ballantine smear inspection method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1854709A CN1854709A (en) | 2006-11-01 |
| CN100587450C true CN100587450C (en) | 2010-02-03 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200510034324A Expired - Fee Related CN100587450C (en) | 2005-04-29 | 2005-04-29 | Freezing Ballantine smear inspection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100587450C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1967196B (en) * | 2006-09-14 | 2010-05-12 | 绵竹市人民医院 | Simplified thin-layer liquid-based cytology cell smeard preparation method |
| EP2433136B1 (en) | 2009-05-19 | 2015-08-26 | Zetiq Technologies Ltd. | Kits for and methods of differential staining of cervical cancer cells and/or tissues |
-
2005
- 2005-04-29 CN CN200510034324A patent/CN100587450C/en not_active Expired - Fee Related
Non-Patent Citations (9)
| Title |
|---|
| HE染色切片染色质量差的原因及处理方法. 宋平，张世华等.诊断病理学杂志，第2卷第1期. 1995 |
| HE染色切片染色质量差的原因及处理方法. 宋平，张世华等.诊断病理学杂志，第2卷第1期. 1995 * |
| 一种新冰冻切片制片方法. 张建国.临床与实验病理学杂志，第10卷第2期. 1994 |
| 不同固定液对冷冻切片HE染色影响的比较. 高美钦，黄雄飞等.福建医科大学学报，第35卷第4期. 2001 |
| 不同固定液对冷冻切片HE染色影响的比较. 高美钦，黄雄飞等.福建医科大学学报，第35卷第4期. 2001 * |
| 冰冻切片2分钟快速染色法. 张毅，付红霞.白求恩军医学院学报，第2卷第2期. 2004 |
| 冰冻切片2分钟快速染色法. 张毅，付红霞.白求恩军医学院学报，第2卷第2期. 2004 * |
| 冰冻切片染色方法的改良. 毕振春.临床与实验病理学杂志，第10卷第4期. 1994 |
| 冰冻切片染色方法的改良. 毕振春.临床与实验病理学杂志，第10卷第4期. 1994 * |
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| Publication number | Publication date |
|---|---|
| CN1854709A (en) | 2006-11-01 |
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Granted publication date: 20100203 Termination date: 20110429 |