CN100574794C - A kind of Chinese medicine composition of delaying decrepitude of skin - Google Patents
A kind of Chinese medicine composition of delaying decrepitude of skin Download PDFInfo
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Abstract
The invention provides a kind of Chinese medicine composition of delaying decrepitude of skin, said composition is made up of active component and medically acceptable adjuvant, it is characterized in that what described active component was prepared by following materials of weight proportions: 5～10 parts in Colla Corii Asini, 3～6 parts of the Rhizoma Atractylodis Macrocephalaes, 3～6 parts in Poria, 5～10 parts of Arillus Longans, 2～5 parts of Fructus Amomis.The present composition all has good drug effect to naturally aging of skin and photoaging, but delaying skin naturally-aged and anti-skin photoage; The present composition also can increase the dermal matrix hyaluronic acid, thereby reaches the purpose that increases skin moisture content; The present composition has no side effect, and can take for a long time.
Description
Technical field
The present invention relates to a kind of medical configuration product of delaying decrepitude of skin, being specifically related to Chinese medicine is the Chinese patent medicine of feedstock production.
Background technology
Skin aging shows as in appearance that wrinkle increases, cutis laxa, flexibility decrease etc., along with economic development, social progress, people more pay attention to external image, and delaying decrepitude of skin is people's expectation, also are the focuses of anti aging effect medicine, health product research.
Compare with young skin, old and feeble skin mainly shows as skin and presents the atrophy phenomenon, and collagen fiber and extracellular matrix content descend in the skin.Collagen fiber generate and reduce, and degraded increases, and cause skin tension to descend, and wrinkle increases.Hyaluronic acid is a kind of main component of Skin Cell epimatrix, and richness is amassed the substrate at the prickle cell layer and the corium of epidermis.Hyaluronic acid is a kind of polyanionic macromole, every gram hyaluronic acid can be in conjunction with 500 ml waters, be the most important material that keeps skin moisture content, keep competent moisture can ensure that skin has good extensibility and elasticity in the skin, make skin seem plentiful and exquisite quality.
Skin aging comprises naturally-aged and two kinds of processes of photoaging, natural aging is meant the inevitable naturally-aged process that causes because of the age increase, photoaging then is by (the ultraviolet A of the long wave ultraviolet in the daylight, UVA, wavelength 320～400nm) and ultraviolet B radiation (ultraviolet B, UVB, the long-term radiation damages accumulation of wavelength 290～320nm) and cause the process of skin aging.Natural aging refers to betide the old and feeble pathological changes of non-exposure district skin, and photoaging refers to be exposed to the natural environment middle-ultraviolet lamp and the old and feeble pathological changes that causes.The dominant mechanism of naturally-aged is with age, and the skin cell metabolism function descends gradually, and new crude rubber original fiber and hyaluronic acid descend, and the degradation process of mentioned component is accelerated simultaneously, makes skin atrophy gradually.The dominant mechanism of photoaging is that the ultraviolet in the sunlight can directly activate a large amount of matrix metalloproteinase (MMP) of Skin Cell generation, wherein (effect of stromelysins-I) is the most important with MMP-1 (interstitial collagenase) and MMP-3, the specificity various collagens of degrading quicken skin aging.At people's exposure district skins such as face, natural aging and photoaging are carried out simultaneously, increase the weight of the skin aging pathological changes.Free radical damage all plays a significant role in natural aging and two pathological processes of photoaging.
At present existing multiple the outer of Chinese medicine ingredients that contain put on the skin or delaying decrepitude of skin finished product for oral administration, the Chinese medicine health care product of class wherein for oral administration is supported outer thought according to Chinese medicine with interior, promote the metabolism of skin by the conditioning viscera function, the main benefiting QI and nourishing blood classic prescriptions of invigorating blood circulation that adopts, as TAOHONG SIWU TANG, Radix Angelicae Sinensis decoction for tonifying blood, representative products comprises Mrs's oral liquid, Mei Yuanchun oral liquid etc.; Put the many extracts such as Radix Ginseng, the Radix Astragali, Margarita powder that in cosmetics, add of series products outward on the skin.But the said goods mechanism of action is detected in the drug effect to skin natural aging process, and Shang Weijian has the report of delaying skin photoaging drug effect simultaneously.
Except that the Chinese medicine composition, in delaying skin naturally-aged cosmetics, often add with SOD, hyaluronic acid composition and reach effects such as the free radical resisting peroxidating damages, preserves moisture, above-mentioned cosmetics have certain effect, but the not enough metabolic function that is to promote Skin Cell, in case need not then health-care effect does not promptly have continuity.The method of prevention skin photoage mainly is at exposure portion skin foam removal sunscreen cream, sun screen etc. at present, because the major part that sun-proof articles can protect is the UVB of medium wave band, penetrable long-wave band UVA to dermal layer of the skin can not be protected Wan, though the biologic activity of UVA is not as UVB, but the dosage of UVA is 1000 times of UVB in the daylight, thereby sun-proof articles is difficult to block skin photoage pathology process more satisfactoryly.
Aspect health product for oral administration, as replenishing phytoestrogen with soybean isoflavone soft capsule, vitamin E free radical resisting peroxidating infringement etc., sheep placental extract replenishes a certain amount of hormone and becomes to grade, all has certain delaying skin effect, but its drug effect report also is detected in the effect to naturally aging of skin, does not see the effect report to the photoaging process.
Summary of the invention
The technical problem to be solved in the present invention provides a kind of pharmaceutical composition that has delaying skin natural aging and photoaging effect simultaneously.
The technical scheme that the present invention solves the problems of the technologies described above is:
A kind of Chinese medicine composition of delaying decrepitude of skin, said composition is made up of active component and medically acceptable adjuvant, it is characterized in that what described active component was prepared by following materials of weight proportions: 5～10 parts in Colla Corii Asini, 3～6 parts of the Rhizoma Atractylodis Macrocephalaes, 3～6 parts in Poria, 5～10 parts of Arillus Longans, 2～5 parts of Fructus Amomis.
In the technique scheme, the preferable proportioning of active component is: 8～9 parts in Colla Corii Asini, 3～5 parts of the Rhizoma Atractylodis Macrocephalaes, 3～5 parts in Poria, 5～10 parts of Arillus Longans, 2～5 parts of Fructus Amomis, best proportioning is 8 parts in Colla Corii Asini, 3 parts of the Rhizoma Atractylodis Macrocephalaes, 5 parts in Poria, 5 parts of Arillus Longans, 2 parts of Fructus Amomis.
Chinese medicine composition of the present invention is common peroral dosage form, as oral liquid, capsule, granule etc.
The Chinese medicine composition of delaying decrepitude of skin of the present invention, the preparation method of the active component in the said composition are with Colla Corii Asini animal proteolytic enzyme enzymolysis, and all the other composition waters decoct, and merge two parts medicinal liquid then, get final product.This preparation method specifically is made up of following steps:
(1) gets Arillus Longan, the Rhizoma Atractylodis Macrocephalae, Poria, Fructus Amomi with the water logging bubble of 8～12 times of crude drug amounts 1 hour, decocted 0.5～1 hour, leach decocting liquid, the decocting that adds 6～8 times of crude drug amounts in the filtering residue boiled 0.5～1 hour again, merge 2 times decocting liquid, the ratio that is evaporated to water extract and inventory is 5: 1, leaves standstill 1～2 hour, filters; To add 1～1.5% chitosan solution to the final content of chitosan in the filtrate be 0.15% in stirring down, leaves standstill 6～12h after continuing to stir 15～30min, and filtration is got filtrate and is concentrated into and contains the crude drug that is equivalent to 1～2g in every milliliter of concentrated solution;
(2) with the hot water molten of Colla Corii Asini with 5～8 times of crude drug amounts, add the animal proteolytic enzyme of Colla Corii Asini quality 0.6%, in 55～60 ℃, pH 6.5～7.0 hydrolysis enzyme denaturing after 3～5 hours; To add chitosan solution to the final content of chitosan down be 0.15% in stirring, and leaves standstill 15～30min after continuing to stir 15min, filters, and gets filtrate and be concentrated into and contain the Colla Corii Asini raw material that is equivalent to 1～2g in every milliliter of concentrated solution;
(3) concentrated medicament of combining step (1) and step (2) gained gets final product;
Described chitosan solution is prepared as follows: every 100ml percent by volume is to dissolve 1～1.5g viscosity-average molecular weight in 1% the acetum greater than 500,000 chitosan;
In the said method, the percentage ratio of the unit of not indicating is mass percent.
Colla Corii Asini quality in the prescription of the present invention is sticky, wherein contain compositions such as a large amount of collagen protein, be easy to generate degradation digestive function decline performance under abdominal distention, the appetite after the crowd of weakness of the spleen and stomach takes, collagen protein especially wherein needs could be absorbed by human body by the polypeptide of protease hydrolyzed by small-molecular weight.In view of the above, the present invention adopts protease hydrolyzed technology, is the polypeptide composition of small-molecular weight with the collagen protein enzymolysis of macromolecule, with the degree that promotes that Colla Corii Asini is absorbed by human consumption.
The active component of the present composition carries out prescription according to tcm theory, the traditional Chinese medical science thinks that QI and blood is the keep fit main matter basis of vigor of skin, process along with naturally-aged, the QI and blood degradation is put down in writing as Huangdi's Internal Classics: " five or seven yangming meridians decline, the face becoming withered; send out the beginning and fall ", refer to that the people after 35 years old, engenders the aging at outward manifestation positions such as face, hair, its main cause exhausts relevant with the yangming meridian weakness of QI.And yangming meridian is subordinated to taste, and taste are source of generating QI and blood, so fill blood when the function of paying attention to function of spleen and stomach regulating.The skin photosensitiveness aging aspect, long-term or excessive ultraviolet radiation belongs to the pathogenic heat in the tcm causa morbi, easily the cloudy blood of loss skin.So, according to above-mentioned etiology and pathogenesis, draft nourishing the blood and yin, invigorating spleen and reinforcing stomach is the Therapeutic Principle of delaying skin naturally-aged and photoaging process.Aspect the selection of square medicine, nourishing the blood and yin is selected Colla Corii Asini, and as we's monarch drug, Colla Corii Asini is gentle, and nourishing the blood and yin is called as the panacea that nourishes blood, with the difference of other yin nourishing Chinese medicines be that property is gentle and not cold and cool, take the function that can not suppress yang-energy in the body for a long time.And Colla Corii Asini is processed by donkey skin, contain a large amount of and the affine material of human skin tissue, modern medicine discovery Colla Corii Asini contains the former albumen of collagen protein, gelatin (the multiple aminoacid to the human body beneficial of generation after the hydrolysis that the ossein hydrolysis forms, as tryptophan, lysine, arginine, histidine etc.), glycosaminoglycan (is aminopolysaccharide, be mainly hyaluronic acid), and fiber mucin, Fe, Mn, 27 kinds of trace element such as Zn, Cu, meet the Therapeutic Method of the traditional Chinese medical science " dirty " with dirty benefit.The Rhizoma Atractylodis Macrocephalae, Poria invigorating the spleen and benefiting QI rouse oneself the function of taste, to promote the generation of the cloudy liquid of QI and blood, as ministerial drug; The Arillus Longan nourishing blood and invigorating qi, the effect of auxiliary Colla Corii Asini performance nourishing the blood and yin is as adjuvant drug; Fructus Amomi nature and flavor fragrance, the performance regulate the flow of vital energy and in effect, as messenger drug.
The present invention's protease hydrolysis Colla Corii Asini, making the macromolecule collagen protein composition in the Colla Corii Asini effective ingredient is polypeptide by the protease hydrolyzed process degradation, can strengthen the effect that Colla Corii Asini is absorbed by the body; The Chinese medicine that cooperates function of spleen and stomach regulating simultaneously promotes the absorption of Colla Corii Asini enzymolysis solution, and promotes the metabolic activity of Skin Cell.The experiment proved that the present composition has following effect: naturally aging of skin and photoaging are all had good drug effect, and mainly showing to increase by two kinds of main components of aging skin substrate: collagen and hyaluronic acid increase skin moisture content; Suppress the expression of stromelysins in the skin; And the present invention has no side effect, and can take for a long time.
To prove the technique effect that the present invention has by zoopery below.
1, laboratory animal
30 of cleaning level NIH mices, female available from Guangdong Medical Lab Animal Center, body weight 18～22g is divided into 3 groups at random, 10 every group, is respectively normal group, Ageing Model group, treatment group of the present invention.
2, the processing method of each experiment grouping mice
Ageing Model group: adopt the ultraviolet induction photoaging, induce the free radical damage simulating nature aging with the D-galactose.Stick with paste with 15% sodium sulfide and to slough the back by hair, expose the skin of about 3cm x 3cm, shave off the undercoat of new length with shaver every day, every day the subcutaneous injection 12.5%D-of nape portion galactose 0.24mL30g
-1, dosage is 1000mgkg
-1D
-1Use the irradiation of ultraviolet B radiation lamp (UVB) and long wave ultraviolet lamp (UVA) simultaneously, the preceding 20d of UVA (365nm) exposure dose every day is 620 μ wcm
2* 60min=2.23Jcm
2, back 20d exposure dose every day is 620 μ wcm
2* 120min=4.46Jcm
2, the preceding 20d of UVB (254nm) exposure dose every day is 11.6 μ wcm
2* 60min=0.041Jcm
2, back 20d exposure dose every day is 11.6 μ wcm
2* 120min=0.082Jcm
2Continue 40d altogether, the total irradiation dose of UVA is 133.8Jcm
2, the total irradiation dose of UVB is 2.46Jcm
2The present invention irritates the oral liquid of stomach embodiment 1 simultaneously, and wherein active constituent content is 1g/ml, and every Mus dosage every day is equivalent to 10 times of adult's (60kg) clinical consumption for being equivalent to the 100mg crude drug.The normal control group: unhairing is handled the same, and every day, capacity normal saline such as nape portion subcutaneous injection was refused ultra-vioket radiation, gave distilled water every day and irritated stomach.
3, instrument and reagent
Shanghai day ring T SN-695B type intelligence is put and is exempted from cut refiner, 3300PRD ultraviolet-uisible spectrophotometer in γ measuring instrument, LIBROR AEG220 type electronic analytical balance, the IKAT18.Hyaluronic acid radioimmunity detection kit is available from Beijing North biotechnology research institute, and malonaldehyde (M DA) is measured test kit and built up the biological reagent institute available from Nanjing.
4, index detects
(1) collagen is measured and is adopted chloramine-t method, get 0.5cm * 0.5cm skin and place 10mL tool plug scale test tube, add 2ml 6MHCl mixing, behind the close plug, place 125 ℃ of thermostatic drying chamber 3h, be chilled to room temperature after the taking-up, transfer PH to 5～7 with 10M, 0.1MNaOH, thin up is to 5ml, as the sample test liquid.Get 0.2ml and put scale in vitro, add water to 1ml, add the 1ml chloramine-T, shake up, room temperature is placed 20min, adds 1ml and crosses chloric acid (3.15mol/L), shakes up, room temperature is placed 5min, add 10% pair of Dimethylaminobenzene first of 1ml vinegar solution, shake up 75 ℃ of water-bath 10min, cool off room temperature, colorimetric under the 550nm ripple rapidly.Standard curve preparation: take by weighing the 100mg hydroxyproline and be dissolved in the 0.01mol hydrochloric acid, move in the 100ml volumetric flask, be diluted to scale, as storing titer, its concentration is 1000 μ g/ml, gets storage titer 2ml and is diluted to 100ml, as using liquid, its concentration is 20 μ g/ml.Get standard hydroxyproline 2,5,8,10,16,20 μ g and be placed on respectively in the test tube, transfer to 1ml, add the 1ml chloramine-T, the same sample of subsequent operation.
(2) hyaluronic acid contents is measured and is adopted radioimmunology, take off neck execution and respectively organize mice, the about 0.5cm of clip expose portion * 0.5cm skin of back, remove lurid subcutaneus adipose tissue, to remain skin shreds for a short time with ophthalmology, move in the test tube, adding 1ml contains in the phosphate buffer that does not contain calcium ions and magnesium ions of 0.1%Triton X-100, under the condition of ice bath with the homogenate of interior cut refiner, homogenate is at 4 ℃ centrifugal (7000r/min 30min), take out supernatant (containing free type hyaluronic acid),-20 ℃ of storages, for reducing the operate miss that the tissue homogenate inequality causes, measure the protein concentration of each sample homogenate supernatant after centrifugal, protein concentration is measured with the Coomassie brilliant blue method in dilution back, and the hyaluronic acid concentration measured divided by protein concentration, is obtained hyaluronic acid contents in every gram histone (μ g/g histone).
(3) malonaldehyde (MDA) is measured test kit and is built up the biological reagent institute available from Nanjing, and strictness is undertaken by the test kit description.
(4) (this experiment of mensuration of stromelysins-I) is respectively organized mouse skin MMP-1, MMP-3 expression product (being brown yellow granule) difference with the semiquantitative method of SABC for MMP-1 (interstitial collagenase) and MMP-3.The result judges that MMP-1, MMP-3 express when positive, in the visible endochylema the different brown yellow granule of the depth is arranged in section, according to the average staining power of all specimen, by do not have to be divided into by force-,+, ++, +++level Four.
5, each data of statistical procedures are represented with mean ± standard deviation, carry out one factor analysis of variance with SPSS software.
6, experimental result
(1) respectively organizes the variation of hydroxyproline in the mouse skin, hyaluronic acid, mda content
Each result of variations of organizing hydroxyproline in the mouse skin (HYP), hyaluronic acid (HA), malonaldehyde (MDA) content sees Table 1.Hydroxyproline, hyaluronic acid contents are starkly lower than the normal control group in the Ageing Model group mouse skin, and malonaldehyde is apparently higher than normal control group (P＜0.01); After treatment by Chinese herbs, have in various degree and recover, hydroxyproline, hyaluronic acid, mda content return to normal control group level, with the Ageing Model group notable difference (P＜0.01) is arranged, as seen the present invention's energy antagonism free radical damage is repaired the topmost two kinds of compositions of dermal matrix---collagen and hyaluronic acid.Each is organized the phenomenon that has no side effect and takes place.
Table 1 is respectively organized the variation of hydroxyproline in the mouse skin, hyaluronic acid, mda content
*Compare P＜0.01 with the normal control group;
△ △Compare P＜0.01 with the Ageing Model group.
(2) respectively organize the variation of MMP-1 and MMP-3 expression product in the mouse skin
Ultraviolet in the daylight can activate skin matrix metalloproteinase (matrix metalloproteinases, MMPs) high expressed excessively, wherein (the most important specificity of effect of stromelysins-I) the various collagens of degrading quicken skin aging with MMP-1 (interstitial collagenase) and MMP-3.Thereby the rising of MMP-1 and MMP-3 expression product is the sign that the photoaging pathological change is different from the naturally-aged pathological process in the skin.The result is as shown in table 2, the Ageing Model mice is under the irradiation of long-term UVA, UVB stimulates, MMP-1, MMP-3 expression product (being brown yellow granule) be than the normal group showed increased in the skin, is distributed widely under the epidermis, skin corium, and express in segment sets such as hair follicle; After the present invention's treatment, this pathological change is significantly alleviated.
Table 2 is respectively organized MMP-1 in the mouse skin, MMP-3 change of Expression
Carry out statistical analysis with X 2 test,
*Compare P＜0.01 with the normal control group;
△ △Compare P＜0.01 with the Ageing Model group.Clinical pharmacodynamic experiment
The object of observation: select 30 routine dry skin experimenters, the age was controlled between 30～40 years old, did not have each systemic disease through health check-up, and experimenter's skin is dry skin, and dry and astringent tight sense is often arranged after washing one's face at ordinary times, and skin often has desquamation when dry weather.
Instrument: digital moisture of skin detector
Experimental drug: Chinese medicine composition of the present invention, dosage form are oral liquid, and concrete production method is referring to embodiment 1.
Method of testing: observing season is 10～December in 2006, and all tests are all carried out in the room of constant temperature, constant humidity, temperature (22 ± 1) ℃, humidity (50 ± 5) %.The person of being observed before taking medicinal liquid, take medicinal liquid after two weeks, under quiescent condition, detect skin of forehead moisture.Viewing duration, the experimenter takes present composition oral liquid, every day dosage: 20ml, each 10ml, every day 2 times, totally 2 weeks.At period in a medicine, all objects of observation are not all taken other health product or are put skin care item outward on the skin.
The clinical experiment result: the skin moisture content meansigma methods is 46.5% before the treatment, treatment back skin moisture content meansigma methods is 60.7%, through pairing T inspection statistics significance statistical significance (p＜0.05) is arranged, performances such as part experimenter pachylosis, corse sweat pore simultaneously also have improvement.
The specific embodiment
Embodiment 1:(oral liquid)
1, gets Colla Corii Asini 800g, Rhizoma Atractylodis Macrocephalae 300g, Poria 500g, Arillus Longan 500g, Fructus Amomi 200g by best proportioning, above-mentioned Chinese medicine decocting except that Colla Corii Asini is got 2 times, amount of water is respectively 10 times, 8 times volumes of crude drug amount, decoct 1h respectively, merge 2 times and take liquid, concentrating under reduced pressure, being concentrated into the water extract is 5: 1 with the ratio that feeds intake, standing over night, the filter cloth coarse filtration is got filtrate.1.5% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain the 1g raw material of Chinese medicine in every milliliter of concentrated solution.
2, with 800g Colla Corii Asini hot water molten, the ratio of Colla Corii Asini and water is 1: 5, adds the animal proteolytic enzyme of Colla Corii Asini quality 0.6%, 55 ℃ of hydrolysis temperatures, and pH value 6.5～7.0, hydrolysis time 3 hours is adjusted 1 pH value every 1h therebetween.Enzymolysis post-heating to 90 ℃ is kept 10 minutes enzyme denaturing.1.5% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain 1g Colla Corii Asini raw material in every milliliter of concentrated solution.
4, merge above-mentioned two kinds of liquid, concentrating under reduced pressure, to the water extract be 1: 1 with the ratio that feeds intake.
5, add potassium sorbate, making its final concentration is 0.15%.The circulation steam sterilization.
Instructions of taking: oral, every day dosage: 20ml, each 10ml.
Embodiment 2:(oral liquid)
1, Colla Corii Asini 1000g, Rhizoma Atractylodis Macrocephalae 600g, Poria 300g, Arillus Longan 500g, Fructus Amomi 500g, above-mentioned Chinese medicine decocting except that Colla Corii Asini is got 2 times, amount of water is respectively 10 times, 8 times volumes of crude drug amount, decoct 1h respectively, merge 2 times and take liquid, concentrating under reduced pressure, being concentrated into the water extract is 5: 1 with the ratio that feeds intake, standing over night, the filter cloth coarse filtration is got filtrate.1.5% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain the 1g raw material of Chinese medicine in every milliliter of concentrated solution.
2, with 1000g Colla Corii Asini hot water molten, the ratio of Colla Corii Asini and water is 1: 5, adds the animal proteolytic enzyme of Colla Corii Asini quality 0.6%, 55 ℃ of hydrolysis temperatures, and pH value 6.5～7.0, hydrolysis time 3 hours is adjusted 1 pH value every 1h therebetween.Enzymolysis post-heating to 90 ℃ is kept 10 minutes enzyme denaturing.1.5% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain 1g Colla Corii Asini raw material in every milliliter of concentrated solution.
4, merge above-mentioned two kinds of liquid, concentrating under reduced pressure, to the water extract be 1: 1 with the ratio that feeds intake.
5, add potassium sorbate, making its final concentration is 0.15%.The circulation steam sterilization.
Instructions of taking: oral, every day dosage: 20ml, each 10ml.
Embodiment 3:(oral liquid)
1, Colla Corii Asini 500g, Rhizoma Atractylodis Macrocephalae 600g, Poria 600g, Arillus Longan 1000g, Fructus Amomi 500g, above-mentioned Chinese medicine decocting except that Colla Corii Asini is got 2 times, amount of water is respectively 10 times, 8 times volumes of crude drug amount, decoct 1h respectively, merge 2 times and take liquid, concentrating under reduced pressure, being concentrated into the water extract is 5: 1 with the ratio that feeds intake, standing over night, the filter cloth coarse filtration is got filtrate.1.5% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain the 1g raw material of Chinese medicine in every milliliter of concentrated solution.
2, with 500g Colla Corii Asini hot water molten, the ratio of Colla Corii Asini and water is 1: 5, adds the animal proteolytic enzyme of Colla Corii Asini quality 0.6%, 55 ℃ of hydrolysis temperatures, and pH value 6.5～7.0, hydrolysis time 3 hours is adjusted 1 pH value every 1h therebetween.Enzymolysis post-heating to 90 ℃ is kept 10 minutes enzyme denaturing.1.5% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain 1g Colla Corii Asini raw material in every milliliter of concentrated solution.
4, merge above-mentioned two kinds of liquid, concentrating under reduced pressure, to the water extract be 1: 1 with the ratio that feeds intake.
5, add potassium sorbate, making its final concentration is 0.15%.The circulation steam sterilization.
Instructions of taking: oral, every day dosage: 20ml, each 10ml.
Embodiment 4:(granule)
1, Colla Corii Asini 500g, Rhizoma Atractylodis Macrocephalae 300g, Poria 300g, Arillus Longan 1000g, Fructus Amomi 500g, above-mentioned Chinese medicine decocting except that Colla Corii Asini is got 2 times, amount of water is respectively 10 times, 8 times volumes of crude drug amount, decoct 1h respectively, merge 2 times and take liquid, concentrating under reduced pressure, being concentrated into the water extract is 5: 1 with the ratio that feeds intake, standing over night, the filter cloth coarse filtration is got filtrate.1% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain the 2g raw material of Chinese medicine in every milliliter of concentrated solution.
2, with 500g Colla Corii Asini hot water molten, the ratio of Colla Corii Asini and water is 1: 6, adds the animal proteolytic enzyme of Colla Corii Asini quality 0.6%, 60 ℃ of hydrolysis temperatures, and pH value 6.5～7.0, hydrolysis time 4 hours is adjusted 1 pH value every 1h therebetween.Enzymolysis post-heating to 90 ℃ is kept 10 minutes enzyme denaturing.1% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain 2g Colla Corii Asini raw material in every milliliter of concentrated solution.
4, merge above-mentioned two kinds of liquid, be evaporated to extractum.
5, in Chinese medical concrete in extractum: the ratio of excipient=1: 3.5 adds excipient, makes granule by conventional method.Wherein, each 1/3rd forms described excipient by soluble starch, dextrin, lactose.
6, adopt the aluminum-plastic composite membrane packing, every packing has the 10g content.
Instructions of taking: warm boiled water, every day dosage: 20g, divide secondary to take, each 10g.
Embodiment 5:(capsule)
1, Colla Corii Asini 1000g, Rhizoma Atractylodis Macrocephalae 500g, Poria 500g, Arillus Longan 500g, Fructus Amomi 300g.Above-mentioned Chinese medicine decocting except that Colla Corii Asini is got 2 times, and amount of water is respectively 10 times, 8 times volumes of crude drug amount, decocts 1h respectively, and merge 2 times and take liquid, concentrating under reduced pressure, being concentrated into the water extract is 5: 1 with the ratio that feeds intake, standing over night, the filter cloth coarse filtration is got filtrate.1% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain the 2g raw material of Chinese medicine in every milliliter of concentrated solution.
2, with 1000g Colla Corii Asini hot water molten, the ratio of Colla Corii Asini and water is 1: 8, adds the animal proteolytic enzyme of Colla Corii Asini quality 0.6%, 55 ℃ of hydrolysis temperatures, and pH value 6.5～7.0, hydrolysis time 5 hours is adjusted 1 pH value every 1h therebetween.Enzymolysis post-heating to 90 ℃ is kept 10 minutes enzyme denaturing.1% chitosan (viscosity-average molecular weight＞500,000 that are made into 1% acetic acid, the heating hydrotropy), eventually amount is 0.15% (in chitosan, W/W), add in the medicinal liquid, continue to stir 15min in stirring down, room temperature leaves standstill 24h, filtrate is got in filtration, is concentrated into to be equivalent to contain 2g Colla Corii Asini raw material in every milliliter of concentrated solution.
4, merge above-mentioned two kinds of liquid, be evaporated to Chinese medical concrete.
5, in Chinese medical concrete in extractum: the ratio of excipient=1: 3.5 adds excipient, makes granule by conventional method.Wherein, each 1/3rd forms described excipient by soluble starch, dextrin, lactose.
6, fill with No. 0 capsulae vacuus, every capsule contains content 0.6g, every day dosage: each 6, every day 3 times.
Claims (4)
1, a kind of Chinese medicine composition of delaying decrepitude of skin, said composition is made up of active component and medically acceptable adjuvant, it is characterized in that what described active component was prepared by following materials of weight proportions: 5～10 parts in Colla Corii Asini, 3～6 parts of the Rhizoma Atractylodis Macrocephalaes, 3～6 parts in Poria, 5～10 parts of Arillus Longans, 2～5 parts of Fructus Amomis.
2, the Chinese medicine composition of a kind of delaying decrepitude of skin according to claim 1 is characterized in that the proportioning of described active component raw material is: 8～9 parts in Colla Corii Asini, 3～5 parts of the Rhizoma Atractylodis Macrocephalaes, 3～5 parts in Poria, 5～10 parts of Arillus Longans, 2～5 parts of Fructus Amomis., 3, the Chinese medicine composition of a kind of delaying decrepitude of skin according to claim 1, it is characterized in that the proportioning of described active component raw material is: 8 parts in Colla Corii Asini, 3 parts of the Rhizoma Atractylodis Macrocephalaes, 5 parts in Poria, 5 parts of Arillus Longans, 2 parts of Fructus Amomis.
4, the preparation method of the active component of the Chinese medicine composition of claim 1,2 or 3 described a kind of delaying decrepitude of skin, this method is made up of following steps:
(1) gets Arillus Longan, the Rhizoma Atractylodis Macrocephalae, Poria, Fructus Amomi with the water logging bubble of 8～12 times of crude drug amounts 1 hour, decocted 0.5～1 hour, leach decocting liquid, the decocting that adds 6～8 times of crude drug amounts in the filtering residue boiled 0.5～1 hour again, merge 2 times decocting liquid, the ratio that is evaporated to water extract and inventory is 5: 1, leaves standstill 1～2 hour, filters; To add 1～1.5% chitosan solution to the final content of chitosan in the filtrate be 0.15% in stirring down, leaves standstill 6～12h after continuing to stir 15～30min, and filtration is got filtrate and is concentrated into and contains the crude drug that is equivalent to 1～2g in every milliliter of concentrated solution;
(2) with the hot water molten of Colla Corii Asini with 5～8 times of crude drug amounts, add the animal proteolytic enzyme of Colla Corii Asini quality 0.6%, in 55～60 ℃, pH6.5～7.0 hydrolysis enzyme denaturing after 3～5 hours; To add chitosan solution to the final content of chitosan down be 0.15% in stirring, and leaves standstill 15～30min after continuing to stir 15min, filters, and gets filtrate and be concentrated into and contain the Colla Corii Asini raw material that is equivalent to 1～2g in every milliliter of concentrated solution;
(3) concentrated medicament of combining step (1) and step (2) gained gets final product;
Described chitosan solution is prepared as follows: every 100ml percent by volume is to dissolve 1～1.5g viscosity-average molecular weight in 1% the acetum greater than 500,000 chitosan;
In the above-mentioned steps, the percentage ratio of the unit of not indicating is mass percent.
5, the Chinese medicine composition of claim 1,2 or 3 described a kind of delaying decrepitude of skin, said composition is oral liquid, capsule or granule.
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| CN103462873B (en) * | 2013-09-26 | 2015-08-19 | 广州丹奇日用化工厂有限公司 | Skin care item of a kind of looks improving and the skin nourishing and preparation method thereof |
| CN103462855B (en) * | 2013-09-26 | 2015-01-28 | 广州丹奇日用化工厂有限公司 | Cosmetic containing American ginseng and longan and preparation method thereof |
| CN104905110A (en) * | 2015-06-16 | 2015-09-16 | 青岛浩大海洋保健食品有限公司 | Health-care chitosan capsule with senescence delaying effect |
| CN109475580A (en) * | 2016-07-21 | 2019-03-15 | 东阿阿胶股份有限公司 | Sebum amount dose and hyaluronic acid generate promotor |
| CN107198766B (en) * | 2017-05-18 | 2020-09-22 | 南京中科药业有限公司 | Traditional Chinese medicine composition for beautifying and preparation method and application thereof |
| CN113116801B (en) * | 2019-12-26 | 2023-01-24 | 华熙生物科技股份有限公司 | Composition for improving skin sub-health state and preparation method thereof |
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Non-Patent Citations (4)
| Title |
|---|
| 中医药治疗黄褐斑研究进展. 苏燕晨.河北中医，第27卷第1期. 2005 |
| 中医药治疗黄褐斑研究进展. 苏燕晨.河北中医，第27卷第1期. 2005 * |
| 中药延缓皮肤衰老的研究现状. 王倩等.中国美容医学，第15卷第2期. 2006 |
| 中药延缓皮肤衰老的研究现状. 王倩等.中国美容医学，第15卷第2期. 2006 * |
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