CN100569374C - Be used to receive and discharge the device of fluid - Google Patents

Be used to receive and discharge the device of fluid Download PDF

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Publication number
CN100569374C
CN100569374C CNB2005800149373A CN200580014937A CN100569374C CN 100569374 C CN100569374 C CN 100569374C CN B2005800149373 A CNB2005800149373 A CN B2005800149373A CN 200580014937 A CN200580014937 A CN 200580014937A CN 100569374 C CN100569374 C CN 100569374C
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backstop
piston
described device
suction
cylinder
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CN1980740A (en
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伯恩德·斯坦布伦纳
罗杰·斯坦布伦纳
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Abstract

Be used to receive and discharge the device of fluid, have a plurality of piston-cylinder-unit (1) that are arranged on a piston plate (2) and the air cylinder board (3); wherein in cylinder (1 "), move reception and the discharge of carrying out fluid by piston (1 ') with piston (1 ') and cylinder (1 "), piston (1 ') moving then in cylinder (1 ") realized by means of piston plate (2) that produces by driving mechanism (6) and dull and stereotyped guiding mechanism and the variable in distance between the air cylinder board (3); wherein upward backstop (4) and a following backstop (5) can be determined volume by one; and upward have at least one can regulate by means of at least one governor motion (8) in backstop (4) and the following backstop (5), wherein by means of the collimation in last backstop (4) and following backstop (5) realization piston plate (2) and the superincumbent stop position of air cylinder board (3) (9) and the following stop position (10).

Description

Be used to receive and discharge the device of fluid
Technical field
The present invention relates to a kind of device that is used to receive and discharge fluid, have a plurality of piston-cylinder-unit that are arranged on a piston plate and the air cylinder board with piston and cylinder, wherein receive and discharge fluid by piston moving in cylinder, piston moving in cylinder then by means of changing realization by a driving mechanism and a dull and stereotyped spacing that produces perpendicular to the dull and stereotyped guiding mechanism of plate level, wherein volume can be determined by the backstop with below one above one, can regulate at least one backstop wherein by means of at least one governor motion here.
Prior art
In the biological study in modern times, use high-throughput method more and more, just in fact always being carried out to batch processing with the suction rapid closely-related sample of moving one's steps.Sample a standardized size have 96,384 grooves or even the flat board of 1563 grooves on handle.In fact, needed a large amount of suction is moved one's steps and can't have been handled with traditional manual 8 passages or 12 passage pipettes suddenly.People use the suction with 96 or 384 passages to move automechanism (Beckman-Coulter, Gilson, Tecan, Zymark, Quiagen, Robbins, Zinsser, Perkin Elmer or the like) for this reason.But these instruments are extremely expensive, need much place areas, and require to be personnel's expense of programming, the operation and maintenance input is very big.Suction in these automations moves in the system, the main shaft drives mechanism of piston unit by quick rotation with parallel conductive extremely accurately to mode move up and down.Move volume by accurately determining in the Z-position that stops at an appointment to inhale.
A kind of manually operated or move in the system by means of the suction of a simple drive mechanism, a kind of such have essential fast and the accurate main shaft drives mechanism of the speed of mainshaft synchronously is not a kind of suitable solution because it needs very high machinery expenses or needs very high control and driving expense.
At US 5,540, a kind of mechanical device that simply has the feature of the described type of beginning is disclosed in 889.In this device, piston plate is arranged in the middle manual drives bar of piston plate by one and moves with respect to air cylinder board.The backstop that one adjustable is used to determine to remain to be inhaled the volume that moves is positioned at the manual drives bar.Collimation between piston plate and the air cylinder board at this by the guiding of manual drives bar and also realize in case of necessity by the guiding of piston in cylinder.This principle and general disclosed multichannel pipette (8 passages mentioned above or 12 passage pipettes) are corresponding.To handle point-device volume if move past in the journey a suction, so a kind of such mechanical device is not enough, because it is opposite with the main shaft drives mechanism that is controlled of the described automatics of beginning, described guiding mechanism can't guarantee absolute collimation, thereby also can't guarantee to remain to be inhaled the accurate identical volume of the fluid that moves, guarantee that wherein the precision of dull and stereotyped guiding mechanism needs high expense thereby also very uneconomical.This problem is along with the number of piston-cylinder-unit and therefore increase along with the size of flat board, thereby utilizes described guiding mechanism or can only operate seldom cylinder-piston-unit simultaneously, or must abandon the accuracy of volume.
Summary of the invention
Therefore, task of the present invention is, makes the device of the described kind of beginning be suitable for the accurate volume of the piston-cylinder-unit of greater number is handled with seldom technical fee.
By the present invention, this task so solves, make that the surperficial collimation to plate level requires to guarantee that the reception volume of piston-cylinder-unit is identical with displaced volume under the lower situation in the process that moves in dull and stereotyped guiding mechanism, method is like this structure and arrange backstop up and down, thereby obtains collimation in the dull and stereotyped superincumbent and following stop position by means of backstop up and down.
Be by solution route of the present invention, accurate volume suction in-migration said there is no need to move past moving that all pistons of assurance are unified in the journey, just guarantee the permanent collimation of piston plate and air cylinder board in whole suction.Or rather, if all cylinder-pistons-unit is in that just in time two moment are in the same position: in the final position below and in the final position on, this point guaranteed by backstop up and down, and is just enough the precision of this volume that institute is received or discharges so.These two positions are equivalent to the beginning and the end of fluid reception or are equivalent to beginning and the end that fluid is discharged on the contrary, if these two positions accurately match each other, whether piston plate and air cylinder board be exactly parallel just inessential concerning the accuracy of volume betwixt so, because this inhomogeneities is at the latest along with in the impact-not only convection cell reception just of going up separately on backstop and the following backstop, and convection cell discharge-is compensated once more.Accuracy is moved driving mechanism with suction thus and is had nothing to do.
Satisfied in the present invention thus and carried out accurate and reproducible suction and move needed two conditions:
The displacement of piston-cylinder-unit and thus the size of volume can accurately regulate by the following spacing between backstop and the last backstop.
Piston plate and air cylinder board by backstop up and down concerning the determining of volume, have decisive action begin and end position on the absolute parallel orientation.
Same displacement is run through with this in all piston-cylinder-unit, not only accurately determines to inhale thus to move volume, and guarantees that it all is identical that this suction moves volume each unit in the single piston-cylinder-unit of system.Correspondingly phase shift volume is together inhaled in each unit, wherein can very accurately regulate all volumes simultaneously.In addition, operating physical force also is in the realistic scope.
Utilize the device of introducing here, provide a kind of expense cheap scheme first, the suction that is used to the work of high throughput to implement to carry out a large amount of the time moves past journey.Here there is no need to carry out very high investment, and the operating cost of this device and gross weight all are starkly lower than the described automatics of beginning.Especially research institute and the company to little company, mechanism of university or developing country is very beneficial for this.
Utilize by device of the present invention, can fill up the blank between multichannel pipette and the fully automatic system.It make receive simultaneously or discharge a plurality of, be preferably 96 fluid samples and become possibility.Volume can freely be selected and can accurately be regulated.It is easy to learn that suction moves past Cheng Benshen, and match with the operating process of being familiar with from the manually operated pipette in decades to a great extent.
There is no need to use electronic-controlled installation or electric driving mechanism, this does not get rid of the use of such mechanism very simply.Why simple, be because not only when manual operation, and when motor-driven driving up and down between the backstop uncontrolled moving up and down just be enough to finish the liquid process of moving.Equally, if backstop once regulated collimation up and down, they can utilize a display unit to regulate by simple adjustment movement once so.This system can turn round under the situation that does not have the very high programming operation of expense thus.Therefore can under the situation that need not carry out the training that the time is long, expense is high even by the beginner, operate.As traditional manually operated pipette, also can whether flawless operation be checked to this system with vision at any time.This system is complementary with concrete requirement fast and neatly, and because its size is little equally also can use under the narrow condition in space as in the enterprising enforcement of the workbench of sterilization.
Backstop up and down can be set with different modes.Only importantly, they will guarantee dull and stereotyped collimation, just will guarantee to be in the piston plate in top and the following stop position and the collimation of air cylinder board.If mobile piston plate for example, air cylinder board can or can carry such backstop as backstop down so, only need also then to arrange that is gone up a backstop.Should go up equally backstop itself and also can be one flat plate or it also can be two wire backstops.But advantageously, each in the backstop all has at least three abuts up and down, in the perimeter of the flat board that these abuts are positioned to move.Why needing at least three abuts, is because can determine the exact position on a plane thus.Suction moves past Cheng Tongchang to be carried out by means of working plate, and these working plates have the grid of a quadrature and therefore have quadrangle form.Therefore, people also can select a kind of quadrangle form for piston plate and air cylinder board.Four abuts then just are provided on dull and stereotyped angle, wherein can certainly have used three abuts being mentioned.
Be preferably backstop outfit governor motion up and down, be used to regulate the collimation of stop position up and down.This not only is used for regulating first accurately, and is used for possible readjusting.
Propose in order to carry out volume-adjustment as far as possible simply, so be configured to the governor motion of volume-adjustment, regulate thereby make adjustable abut last or backstop down can carry out height simultaneously.Can carry out volume-adjustment by once unique operation in this way, wherein always guarantee the collimation of piston plate and air cylinder board in the final position, and therefore guarantee the precise volumes of all piston-cylinder-unit.This also can carry out different designs.For example can regulate the abut of backstop up and down by stepping motor.This can be achieved by a kind of control device very simple, that have a unique display.
Another kind of scheme is, by means of a transmission mechanism abut of backstop up and down carried out adjusted in concert.Abut for example can be regulated by leading screw or by the adjustment nut that can rotate on fixing leading screw, and described leading screw or adjustment nut then carry out same moved further by means of odontoid belt and gear by a governor motion.This adjusting can undertaken by a piston plate and an air cylinder board that is resisted against on the corresponding backstop under the situation that is not subjected to the load effect.If for example the direction of piston plate moves or following backstop or its corresponding abut towards a flat board that abuts against, that just should preferably remove flat board in advance, so that guarantee ease for operation and very little power consumption.This point of can certainly taking measures to guarantee is as blocking governor motion or being used for or automatically removing separately flat board down during the governor motion of backstop beginning to operate.But usually, operating personnel will remind and oneself remove flat board, because the flat board that governor motion is abutted against blocks or be difficult to operation.Therefore, should not mention in the same breath the requirement of power consumption and durability with requirement to the main shaft drives mechanism of automatics.Therefore utilize very little power just can press on the device adjusted of the present invention or following backstop or its corresponding abut, also can obtain very high precision thus with simple means.Advantageously, this governor motion has a display unit that shows the volume of being regulated.
Concerning the design of volume-adjustment, importantly, at first exempt all loads relevant, especially exempt driving mechanism and be necessary for to inhale and move past the load that journey applies, and therefore can obtain very high precision with expending few mode with power for governor motion.
If compare the obviously more piston-cylinder-unit of needs with a kind of 8 passages or 12 passage pipettes, that just preferred this device that uses, and this piston-cylinder-unit is arranged with grille-like, for example have 96 unit, will go wrong because this point is placed on this device on the working plate of an operating position with same quantity.Therefore propose, with piston plate, air cylinder board, backstop and driving mechanism are arranged in a suction and move in the head up and down, this suction moves head and is suspended on the support by means of a vertically-guided mechanism.Further advantageously, this support has a detent mechanism and is used for that suction is moved head and is used to inhale the working plate that moves with respect to one and positions, so that make the cell structure of piston-cylinder-unit harmonious with the cell structure of operating position on working plate.Can carry out work as quick as thought in this way, and needn't propose too high request operation skill.
Often there is such demand, promptly utilizes a plurality of working plates to carry out work.For example in order from a container, to receive a kind of fluid and then again it to be discharged on the working plate to be processed.Can consider equally, successively carry out repeatedly fluid reception and discharge work for successfully carrying out work, and needn't always change working plate again and again.Often fluid receiver that also must the stationary grizzly arrangements is for example inhaled and is moved point (vide infra).Receive fluid then, and then discharge this fluid, and remove fluid receiver when finishing.This need arrange four working plates.For this reason, arrange other the detent mechanism that is used for working plate, and the optional cell structure with piston-cylinder-unit of the working plate here is consistent.
There is different schemes to provide a plurality of detent mechanisms for working plate.A suggestion is, detent mechanism is installed on the rotating disk, is used for successively making a plurality of working plates consistent with the grid structural coordination.Another suggestion is, described support has one and is used to inhale the horizontally-guided mechanism that moves head and has a plurality of side by side detent mechanisms that are used for working plate each other below horizontally-guided mechanism, wherein is provided with the location auxiliary member that is used to set grid in horizontally-guided mechanism.Another kind of scheme is, for working plate is provided with a plurality of detent mechanisms, working plate then can for example move towards the position of determining by the location auxiliary member by pallet movably by means of at least one horizontally-guided mechanism that is used for working plate, thereby working plate there can be set the cell structure to piston-cylinder-unit.Last-mentioned scheme also can be used in combination with above-mentioned scheme, and method is to be used for the horizontally-guided mechanism of working plate transverse to being used to inhale the horizontally-guided mechanism that moves head.If people are confined to two detent mechanisms respectively in these two kinds of above-mentioned embodiments, locate the final backstop that auxiliary member can be respectively a horizontally-guided mechanism so.Can position (referring to Fig. 2) to often four essential working plates mentioned above thus.If between backstop, arrange the position location certainly, other position also can be set.
Be preferably and inhale vertical mobile at least one backstop down that is provided with of moving head.This time backstop is used for setting a stop position when mobile downward vertically, can carry out desired suction and move past journey in this stop position, because be that fluid receives or discharges and is provided with the correct height position with respect to the corresponding work plate.Therefore same very advantageously, described at least one be used to inhale the following backstop of moving head and can regulate, be used for being adjusted in the immersion depth of the fluid receiver of working plate.If being in detent mechanism, for example a plurality of working plates are used to inhale below the horizontally-guided mechanism that moves head, so certainly for each detent mechanism is provided with such backstop, so that the correct height that exceeds the relevant work plate can be set.
For simply successfully working, propose at this, the vertically-guided mechanism that moves head for suction sets a weight balancing mechanism, and the size of this weight balancing mechanism makes and can carry out easily vertically moving with very little power consumption.It can be a counterweight at this.But preferred spring that can significantly not increase the gross weight of device of use of recommending.Especially suitable at this is a helical spring, because this spring has the characteristic curve that is almost straight line, being used for vertical power consumption of moving thus is identical on whole motion track.For safety, can for suction move the head vertically-guided mechanism set a catching device that very high acceleration is reacted (Fallsperre) or the brake that falls.This helps being avoided descending, and avoids occurring thus accident.If for example spring ruptures and just may cause such accident quickly.Here also can use a kind of hydraulic pressure or pneumatic catching device or the brake that falls, for example pneumatic spring.
In addition concerning alleviating work advantageously, the driving mechanism that is used for piston-cylinder-unit can be operated by a hand lever with drive mechanism.The drive mechanism here can be a kind of corresponding lever drive mechanism or a kind of gear transmission structure or tooth bar drive mechanism or these combination of elements.Can certainly consider other scheme.
Because air cylinder board preferably is positioned on the certain height being used to respect to working plate and receives fluid or discharge fluid,, set driving mechanism thereby be preferably piston plate so advantageously the flat board of Yi Donging is a piston plate.This driving mechanism can be with the stop position of piston plate shift-in below it then, and top stop position then retracted it by back-moving spring.This just makes work become simple especially and prevents that piston plate from resting in the uncertain position.
Can make piston keep sealing by means of seal with respect to cylinder.The benefit of doing like this is, do not have so high required precision aspect piston and the cylinder, and guarantee sealing on very high degree, and allowing in adjustment process piston plate and air cylinder board that to a certain degree malalignment is arranged, and can not cause chucking.Seal can be the sealing ring that is arranged on the piston.But air cylinder board also can be divided in the horizontal direction and come and embed elaxtic seal, thereby can regulate cylinder bore at the sealing position by the mechanism that is used to compress flat part, and wherein piston moves in the scope at sealing position.At this, encapsulant can be the porous plate of being made by elastomeric material, perhaps can set a cyclic spring seal for each single cylinder.The advantage of this design is, can regulate and can readjust the contact of seal on piston.
Can arrange on the bottom of cylinder also that perhaps being used for the socket fluid receiver especially inhales the sleeve pipe that moves point for cylinder directly is equipped with suction inlet.Only these sleeve pipes are wetting by fluid institute thus, and piston-cylinder-unit is as suction apparatus, and these suction apparatus produce the low pressure that is suitable for sucking, and by moving downward the fluid of discharging in the fluid receiver.In this way, only need to change fluid receiver before after a procedure or in replacing, remaining to be inhaled the fluid that moves.Be used for the sleeve pipe of fluid receiver, just for example small tubes or suction move point, can be furnished with elastic ring, even fluid receiver or the suction diameter that moves point not too accurately also can guarantee fixing reliably and be sealed and matched thus.
Move point, whole grid for example in order to fix a large amount of such fluid receivers or suction quickly, need be by means of a certain size power.Therefore propose, a kind of force transmission mechanism is set, it moves head with suction and is in fluid receiver bearing in the detent mechanism, especially inhales and move that the apical branch seat pushes or oppositely extruding towards one, is used for fixing fluid receiver, is particularly useful for fixing the suction and moves point.Force transmission mechanism for example can be a kind of drive link, and this drive link moves between the head in described support and suction and works, and with suction move head towards the fluid receiver bearing, especially move the apical branch seat and push towards suction.By a kind of such force transmission mechanism, just there is no need to adopt to inhale for this reason and divert from one use to another driving mechanism, thereby needn't design this driving mechanism for so high power.
In addition advantageously, a fluid receiver stripping attachment or suction are set and move sharp stripping attachment.This stripping attachment can be a kind of lever, that be arranged in air cylinder board below and vertically moving porous plate.At this, the hole above it must be greater than sleeve pipe, but moves the hole of point less than fluid receiver or suction, so that it can be pulled down from bearing.
Move point in order to handle described fluid receiver such as suction, preferably can discharge residual fluid.Propose, following backstop is detent recesses for this reason, and it has determined the lift that plays a decisive role concerning determining fluid volume, and method is that it makes piston plate and air cylinder board parallel orientation.Then, piston plate that moves and air cylinder board are used to discharge the residual fluid that may be detained under the situation that has overcome chucking power or operated under the situation of release member of detent recesses and can continue to move a segment distance.
A kind of improvement project according to this device, for portion is discharged the fluid that is received aly, between upper and lower backstop, arrange the middle backstop of at least one detent recesses shape, this centre backstop makes piston plate and air cylinder board parallel orientation equally, and this centre backstop of can jumping over bigger power or when operating a release member.The purposes of one or more described middle backstops is determined fluid volume behind once unique fluid receiving course can be discharged on two or more working plates successively.In the time will supplying with a kind of fluid, can more reasonably work in this way for a plurality of working plates.Certainly the middle backstop of described at least one detent recesses shape also should be regulated, thereby also can accurately determine the volume of the fluid of discharge successively.
Description of drawings
By means of a kind of embodiment shown in the accompanying drawing the present invention is described below.Accompanying drawing illustrates:
Fig. 1 shows the functional structure by device of the present invention;
Fig. 2 shows by device of the present invention and moves the layout of head on a support by means of suction;
Fig. 3 illustrates a kind of possible being used to and inhales the driving mechanism that moves past journey.
The specific embodiment
Fig. 1 illustrates the functional structure by a kind of embodiment of device of the present invention.Piston 1 ' is on the piston plate 2 and cylinder 1 " be on the air cylinder board 3, arranged piston-cylinder-unit 1 by grille-like thus.In shown embodiment, be equipped with corresponding perforation for air cylinder board 3, constitute cylinder 1 thus ".In this way, piston plate 2 moves up once just to produce and once sucks motion, and once discharges motion with regard to producing when piston plate 2 moves down one time.In order to make piston 1 ' with respect to cylinder 1 " keep sealing, can be equipped with a sealing ring 31 respectively for them.In the diagram, for example with dashed lines draw one have piston 1 ', sealing ring 31 and cylinder 1 " piston-cylinder-unit 1.In fact be provided with a plurality of described unit 1, select grid-like layout for this reason.Corresponding to common working plate 16, can for example select a kind of grid with 96 piston-cylinder-unit 1, be used for the operating position on the working plate 16 is operated.Other grid can certainly be set.
Driving mechanism 6-only draws the adjustment movement 35 of drive rod 46-of driving mechanism 6 here with in following stop position 10 of piston plate 2 shift-ins.In this position, a kind of fluid is prepared to be used to suck in piston-cylinder-unit 1.Driving mechanism 6 only needs to close now or a unshowned retaining mechanism need be opened safeties, goes up stop position 9 so that back-moving spring 34 moves up piston plate 2 once more up to one.Moving curve can preestablish in mode repeatably by relative measures.In this position 9, suck a kind of fluid.If then piston plate 2 moves down once more, then fluid will be discharged once more.
Essence of the present invention is that piston plate 2 makes by 1 suction of all piston-cylinder-unit accurately identical with the volume of discharging with the accurate location with respect to air cylinder board 3 in following stop position 10 in last stop position 9.A last backstop 4 and a following backstop 5 just are used for this purposes.As far as possible accurately locate in order to make piston plate 2, last backstop 4 and following backstop 5 comprise abut 4 ', 4 respectively ", 4 " ' and 4 " " or abut 5 ', 5 ", 5 " ' and 5 " ", these abuts lean in the perimeter of piston plate 2 and make piston plate 2 carry out the better parallel orientation thus.(because schematic diagram interrupts, so the abut 4 of the last backstop 4 of the corresponding layout of not drawing " ', 4 " " and the abut 5 of following backstop 5 " ' and 5 " ", but arrow has been pointed out their position).The abut 5 ' of following backstop 5,5 ", 5 " ' and 5 " " only can adjust, be used for making piston plate 2 to carry out accurate parallel orientation at following stop position 10.Can so construct down these abuts 5 ', 5 of backstop 5 ", 5 " ' and 5 " ", thereby they in the stop position 10 that draws, form under backstop 5.But under very high power effect, can continue down to push, be used to realize the extra discharge function above mentioned already just be used for removing the residual fluid of attachment removal.
In contrast, the abut 4 ' of last backstop 4,4 ", 4 " ' and 4 " " not only can adjust, and can regulate simultaneously, so that can regulate the volume of the fluid that remains to receive and discharge.Abut 4 ', 4 that be responsible for to go up backstop 4 ", 4 " ' and 4 " " the transmission mechanism 11 of synchronous location adjusting promptly be used for this adjusting.This transmission mechanism 11 comprises a governor motion 8 that has a regulating wheel 47, can drive a drive 44 by this regulating wheel 47.According to the rotation on regulating wheel 47, on volume display 45, demonstrate the volume of being regulated.By this regulating wheel 47, drive 44 is driving a rooted tooth shape belt 42, and 42 driven in synchronism of this odontoid belt driving wheel 43.These driving wheels 43 are connected with adjustment nut 48 on being bearing in leading screw 41, thereby can their height of adjusted in concert.These adjust nuts 48 at the abut 4 ', 4 that forms backstop 4 on its bottom ", 4 " ' and 4 " ", thereby the abut 4 ', 4 of in this way can adjusted in concert all last backstop 4 ", 4 " ' and 4 " ", and when each the adjusting, all guarantee to produce a piston plate 2 accurately keeps with respect to air cylinder board 3 surperficial parallel on stop position 9.Scheme as an alternative, driving wheel 43 also can be used as drive 44.
For the piston-cylinder-unit 1 of not making dirty, preferably this unit only sucks and discharges air, and fluid does not enter cylinder 1 " in.For this purpose, at cylinder 1 " the bottom on arranged sleeve pipe 25, fluid receiver can be enclosed within on these sleeve pipes 25.The preferred suction moved point 26 as fluid receiver.For the such suction of socket moves point 26, shown unit accurately is placed into suction moves on the apical branch seat 28.If then with shown unit toward moving down, it just entangles with sleeve pipe 25 and is in the suction that suction moves in the apical branch seat 28 and moves sharp 26 so.If use other fluid receiver, that just correspondingly carries out this process.For guaranteeing to be clamped in reliably on the sleeve pipe 25, arranged elastic ring 27, these elastic rings 27 also compensate small size difference, thereby always produce needed chucking power.
In addition, a fluid receiver stripping attachment or a suction that is configured to porous plate also is shown moves sharp stripping attachment 30, the described stripping attachment 30 that draws in the position below elastic ring 27 that is to say, suction moves point 26 and disassembled.Move point 26 for fixing the suction, suction is moved sharp stripping attachment 30 move up, for example lean on air cylinder board 3 up to it.Suction can be moved point 26 then and be set on the sleeve pipe 25, and be fixed on the elastic ring 27, because inhale the diameter that the diameter that moves point 26 is slightly less than elastic ring 27.If inhale and to move in sharp stripping attachment 30 position that shift-in drew once more, inhale so and move point 26 and just be removed, and for example fall into one and be the bearing of its preparation.For realizing this disassembling function, the hole in the porous plate must comprise greater than elastic ring 27 greater than sleeve pipe 25, but moves sharp 26 diameter less than suction.
Fig. 2 illustrates the layout of the device shown in Fig. 1 on a support 14.Device shown in Fig. 1 is in a suction and moves in 7, has wherein arranged to be used to inhale the driving mechanism 6 that moves past journey on suction moves 7, can operate this driving mechanism 6 with hand by means of a rotating hand lever 22 and a fixing handle 32.Support 14 comprises a base plate 49 and a frame body 50, has arranged to inhale by means of a vertically-guided mechanism 13 on frame body 50 and has moved 7.In this vertically-guided mechanism 13, can move to inhale and move 7, thereby fluid receiver such as suction move point 26 and can be moved apical branch seat 28 by a fluid receiver bearing or suction and fix, move in the point 26 thereby also fluid can be sucked, and this fluid can be discharged on another working plate 16 once more, on this working plate 16, operating position 33 is arranged, recess for example, fluid then is discharged in these recesses 33.For realizing that these processes carry out correct height location, move 7 and be provided with down backstop 12 for inhaling in advance, can regulate the height of backstop 12 under these according to concrete needs by means of adjusting bolt 21.Following backstop 12 sets for the working plate 16 that can locate there according to detent mechanism 15, and can be adjusted on the working plate 16.
In addition, be provided with a horizontally-guided mechanism 17, be used to make inhale and move 7 and can between two working plates 16 that position by means of detent mechanism 15 that draw, move for suction moves 7.In order to make grid harmonious, be provided with location auxiliary member 18 at the two ends of horizontally-guided mechanism 17 as backstop.
In order to carry out work with more work plate 16, on base plate 49, arranged two transportable pallets 51 by means of horizontally-guided mechanism 19, these two pallets 51 comprise two detent mechanisms 15 that are used for working plate 16 respectively.These detent mechanisms 15 for example can be some recesses, and working plate 16 accurately mates wherein.Pallet 51 can move between two backstop 20 respectively in the horizontally-guided mechanism 19, backstop 20 then so is arranged on the end of this horizontally-guided mechanism 19, thereby all the working plate in detent mechanism 15 16 and suction have been set up the accurate localization coordination between moving 7 in each final position.Certain adjustable detent mechanism 15 also can be used to locate the working plate 16 of different size.Also can change piston plate 2, air cylinder board 3 or inhale and move 7, and can regulate location auxiliary member 18,20 for different grids.
Because be socketed on the sleeve pipe 25 and need very high power consumption for suction being moved point 26 or other fluid receiver, especially if a large amount of suction of socket moves point, for example one has 96 suctions when moving the grid of point, so a drive link 29 just is set.This drive link 29 is arranged in to inhale with rotary way by an articulated elements 36 and moves on 7, and has a support zone 37, the end of drive link 29 is engaged in this support zone 37, is used for stronger power suction being moved 7 extruding down, so that the whole grid that moves point 26 is inhaled in sleeve pipe 25 sockets.Certainly, also can move 7 both sides in suction and be arranged in the drive link 29 that a side is drawn, wherein then the cross bar in the end is responsible for common operation.
Fig. 3 shows one and is used to inhale the possible driving mechanism 6 that moves past journey.Be hinged on by means of an articulated elements 23 to inhale to move on 7 and by means of a transmission bar 38 at this hand lever 22 gyration is passed to a gear 39, this gear 39 also can be a sector gear.This gear 39 is engaged in the tooth bar 40, and tooth bar 40 then is connected with the drive rod 46 that draws in Fig. 1 already.Double-head arrow 35 illustrates the adjustment movement on the piston plate 2, and piston plate 2 here only schematically draws.In this way, just be implemented in corresponding drive mechanism 24 between the adjustment movement 35 of hand lever 22 and piston plate 2.
Schematic diagram only illustrates a kind of possible embodiment of the present invention certainly.Also can construct a kind of such embodiment, for example can under the situation that does not have support 14, be put into above the working plate 16 at device shown in Fig. 1 and this device by means of a corresponding sleeve pipe as a kind of with more simple mode.Can regulate go up backstop 4 or the same backstop 5 down of regulating by step-by-step motor in addition, perhaps not use lever-operated but a kind of simple motor drive mechanism is set.Mobile piston plate 2 and mobile air cylinder board 3 then only are not necessary together to move a working plate that remains to be operated 16 yet, are used to the workplace distance that keeps correct.Also can be according to another kind of improvement project, backstop in the middle of being provided with between last backstop 4 and following backstop 5, these middle backstops mesh in the detent recesses mode and are used for such purpose, and promptly the fluid of Jie Shouing can the discharge successively of a ground.Also can consider other modification.
Reference numerals list
1 piston-cylinder-unit
1 ' piston
1 " cylinder
2 piston plates
3 air cylinder boards
Backstop on 4 (adjustable and adjustable)
4 ', 4 ", 4 " ', 4 " " go up the abut of backstop
5 times backstops (adjustable)
5 ', 5 ", 5 ' ", the 5 " " abut of backstop down
6 are used to inhale the driving mechanism that moves past journey
7 suctions move head
8 are used for the governor motion of backstop
Stop position on 9
10 times stop positions
11 are used for the transmission mechanism of location adjusting synchronously
12 are used to inhale the following backstop of moving head
13 inhale the vertically-guided mechanism that moves head
14 supports
The detent mechanism of 15 working plates
16 working plates
17 inhale the horizontally-guided mechanism that moves head
18 inhale the location auxiliary member (for example backstop) that moves head
19 are used for the horizontally-guided mechanism of the detent mechanism of working plate
20 are used for the location auxiliary member (for example backstop) of detent mechanism
21 adjust bolt
22 hand levers
The articulated elements of 23 hand levers
24 drive mechanisms
25 sleeve pipes
26 fluid receivers or suction move point
27 elastic rings
28 fluid receiver bearings or suction move the apical branch seat
29 drive links
30 fluid receiver stripping attachments or suction move sharp stripping attachment
31 sealing rings
32 handles
Operating position on 33 working plates
34 back-moving springs
35 are used to inhale the adjustment movement of moving
36 drive links move articulated elements on the head in suction
The support zone of 37 drive links on support
38 transmit bar
39 gears or sector gear
40 tooth bars
41 leading screws
42 odontoid belts
43 driving wheels
44 drives
45 volume displays
46 drive rods
47 regulating wheels
48 adjusting nuts
49 base plates
50 bodies
51 pallets

Claims (33)

1. be used to receive and discharge the device of fluid, have a plurality of piston-cylinder-unit (1) that are arranged on a piston plate (2) and the air cylinder board (3); wherein in cylinder (1 "), move reception and the discharge of carrying out fluid by piston (1 ') with piston (1 ') and cylinder (1 "), piston (1 ') moving then in cylinder (1 "); wherein upward backstop (4) and a following backstop (5) are determined volume by one by means of realizing by driving mechanism (6) with perpendicular to piston plate (2) and the variable in distance between the air cylinder board (3) that the dull and stereotyped guiding mechanism of plate level produces; and upward have at least in backstop (4) and the following backstop (5) one to regulate by means of at least one governor motion (8)
It is characterized in that, require under the lower situation in surperficial collimation plate level, the reception volume that guarantees piston-cylinder-unit (1) in the moving process in dull and stereotyped guiding mechanism is identical with displaced volume, method is like this structure and arrange and go up backstop (4) and following backstop (5), thereby by means of the collimation in last backstop (4) and following backstop (5) realization piston plate (2) and the superincumbent stop position of air cylinder board (3) (9) and the following stop position (10).
2. by the described device of claim 1, it is characterized in that, described go up backstop (4) have at least three abuts (4 ', 4 ", 4 " ', 4 " ") and described time backstop (5) have at least three abuts (5 ', 5 ", 5 " ', 5 " "), all these abuts are positioned in the outer surface regions of mobile piston plate (2) and/or air cylinder board (3).
3. by claim 1 or 2 described devices, it is characterized in that described backstop (4) and the following backstop (5) of going up is furnished with the collimation that governor motion is used to regulate top stop position (9) and following stop position (10).
4. by the described device of claim 2, it is characterized in that, like this described governor motion (8) that is used for volume-adjustment of structure, thus adjustable abut of going up backstop (4) (4 ', 4 ", 4 " ', 4 " ") and/or down the abut of backstop (5) (5 ', 5 ", 5 " ', 5 " ") can carry out height adjusting synchronously.
5. by the described device of claim 4, it is characterized in that, the described abut of going up backstop (4) (4 ', 4 ", 4 " ', 4 " ") and/or down the abut of backstop (5) (5 ', 5 ", 5 " ', 5 " ") can regulate by step-by-step motor.
6. by the described device of claim 4, it is characterized in that, by means of a transmission mechanism (11) to the abut of last backstop (4) (4 ', 4 ", 4 " ', 4 " ") and/or down the abut of backstop (5) (5 ', 5 ", 5 " ', 5 " ") carry out adjusted in concert.
7. by claim 1, each described device in 2,4,5 or 6, it is characterized in that described piston plate (2), air cylinder board (3) and last backstop (4) and following backstop (5) are arranged in a suction that is suspended on the support (14) by means of a vertically-guided mechanism (13) and move in the head (7).
8. by the described device of claim 7, it is characterized in that, described support (14) has one and is used for that suction is moved head (7) and is used to inhale the detent mechanism (15) that the working plate (16) that moves positions with respect to one, is used to make the cell structure of the cell structure of piston-cylinder-unit (1) and the operating position (33) on the working plate (16) harmonious.
9. by the described device of claim 8, it is characterized in that be provided with other detent mechanism (15) that is used for working plate (16), these detent mechanisms (15) are mutually harmonious with the cell structure of piston-cylinder-unit (1).
10. by the described device of claim 9, it is characterized in that described detent mechanism (15) is arranged on the rotating disk.
11. by the described device of claim 9, it is characterized in that, described support (14) has one and is used for inhaling the horizontally-guided mechanism (17) move head (7), and below this horizontally-guided mechanism (17), have a plurality of each other side by side the detent mechanism that is used for working plate (16) (15) and the location auxiliary member (18) that is used to set grid.
12. by each described device in the claim 9 or 11, it is characterized in that, a plurality of detent mechanisms (15) that are used for working plate (16) are set, working plate (16) moves towards the position of being determined by location auxiliary member (20) by means of at least one horizontally-guided mechanism (19) that is used for working plate (16), thereby the working plate (16) will the there sets the grid to piston-cylinder-unit (1).
13., it is characterized in that vertical the moving of moving head (7) for described suction is provided with at least one and is used to inhale the following backstop (12) of moving head by the described device of claim 7.
14. by the described device of claim 13, it is characterized in that, regulates described at least one be used to inhale the following backstop (12) of moving, be used for being adjusted in the immersion depth of the fluid receiver of working plate (16).
15. by the described device of claim 13, it is characterized in that, be used to inhale the following backstop (12) of moving head for each detent mechanism (15) that is used for working plate (16) sets one.
16., it is characterized in that the vertically-guided mechanism (13) that moves head (7) for described suction sets a weight balancing mechanism by the described device of claim 7, its size makes carries out vertically moving with very little power consumption easily.
17., it is characterized in that the vertically-guided mechanism (13) that moves head (7) for described suction sets a catching device that very high acceleration is reacted by the described device of claim 16.
18., it is characterized in that the described driving mechanism (6) that is used for piston-cylinder-unit (1) is operated by a hand lever (22) that has drive mechanism (24) by the described device of claim 1.
19., it is characterized in that described driving mechanism (6) works to piston plate (2) by the described device of claim 18.
20., it is characterized in that described driving mechanism (6) is with in the stop position (10) of piston plate (2) shift-in below it by the described device of claim 19, back-moving spring (34) then sends back to it in top stop position (9).
21. by the described device of claim 1, it is characterized in that, make described piston (1 ') keep sealing with respect to described cylinder (1 ") by seal (31).
22. by the described device of claim 21, it is characterized in that, described air cylinder board (3) is divided in the horizontal direction and is come and embed encapsulant, thereby regulates the cylinder bore at sealing position by the mechanism that is used to compress flat part, and wherein piston (1 ') moves in the scope of sealing position.
23. by the described device of claim 8, it is characterized in that, on the bottom of described cylinder (1 "), arranged the sleeve pipe (25) that is used for socket fluid receiver (26).
24., it is characterized in that the described sleeve pipe (25) that is used for fluid receiver is furnished with elastic ring (27) by the described device of claim 23.
25. by the described device of claim 23, it is characterized in that, be provided with a kind of force transmission mechanism, it moves head (7) with suction and pushes or oppositely extruding towards a fluid receiver bearing (28) that is in the detent mechanism (15), is used for socket fluid receiver (26).
26. by the described device of claim 25, it is characterized in that, described force transmission mechanism is a drive link (29), and this drive link (29) moves between the head (7) in support (14) and suction and works, and suction is moved head (7) push towards fluid receiver bearing (28).
27. by the described device of claim 25, it is characterized in that, a fluid receiver stripping attachment (30) be set.
28. by the described device of claim 27, it is characterized in that, described fluid receiver stripping attachment (30) is a lever, that be arranged in air cylinder board (3) below and vertically moving porous plate, and the hole on the porous plate is greater than the hole of sleeve pipe (25), but less than the hole of fluid receiver.
29. by the described device of claim 1, it is characterized in that, described backstop (5) down is detent recesses, thereby also continue to move a segment distance under the piston plate (2) that moves and the situation of air cylinder board (3) chucking power of the stop position (10) below the backstop (5) under overcoming a formation or under the situation of a release member of operation, be used to discharge the residual fluid of delay.
30. by the described device of claim 1, it is characterized in that, for portion is discharged the fluid that is received aly, between last backstop (4) and following backstop (5), arrange the middle backstop of at least one adjustable detent recesses shape, this centre backstop makes piston plate (2) and air cylinder board (3) parallel orientation equally, and with bigger power or by operating a release member this centre backstop of jumping over.
31., it is characterized in that described fluid receiver (26) is to inhale to move point by the described device of claim 23.
32., it is characterized in that described fluid receiver (26) is that suction moves point and fluid receiver bearing (28) is to inhale to move the apical branch seat by the described device of claim 25.
33., it is characterized in that described fluid receiver (26) is to inhale to move point by claim 27 or 28 described devices, described fluid receiver bearing (28) is that suction moves the apical branch seat and described fluid receiver stripping attachment (30) is to inhale to move sharp stripping attachment.
CNB2005800149373A 2004-05-10 2005-04-30 Be used to receive and discharge the device of fluid Active CN100569374C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE102004023517.1 2004-05-10
DE102004023517 2004-05-10
DE102004023690.9 2004-05-13

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CN1980740A CN1980740A (en) 2007-06-13
CN100569374C true CN100569374C (en) 2009-12-16

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CN102602872B (en) * 2012-03-16 2015-09-09 盛司潼 A kind of automatization reagent delivery systems of sequencing device
DE102016111912A1 (en) * 2016-06-29 2018-01-04 Eppendorf Ag Dosing head, dosing device comprising a dosing head and method for dosing by means of a dosing head

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