CN100569086C - The preparation method of rhamnose lactobacillus milk powder - Google Patents
The preparation method of rhamnose lactobacillus milk powder Download PDFInfo
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- CN100569086C CN100569086C CNB2007100175186A CN200710017518A CN100569086C CN 100569086 C CN100569086 C CN 100569086C CN B2007100175186 A CNB2007100175186 A CN B2007100175186A CN 200710017518 A CN200710017518 A CN 200710017518A CN 100569086 C CN100569086 C CN 100569086C
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Abstract
The preparation method of rhamnose lactobacillus milk powder, at first raw material cow's milk being reduced pressure behind high-temperature instantaneous sterilization concentrates, and obtains concentrating breast; Then raw material cow's milk is inoculated Lactobacillus rhamnosus behind high-temperature instantaneous sterilization, and add jerusalem artichoke powder heat insulating culture, obtain activated spawn; Activated spawn is made an addition to concentrated Ruzhong, carry out homogeneous and obtain concentrating newborn mixture; With the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder.Because the present invention adopts the jerusalem artichoke powder to increase microbial inoculum as Lactobacillus rhamnosus, broken through (EAT 130-160 ℃ of high temperature spray-drying technology, temperature of outgoing air 70-75 ℃) the middle low guardian technique difficult problem of Lactobacillus rhamnosus survival rate, Lactobacillus rhamnosus viable count 〉=1 * 10 in the finished product milk powder that makes
7Cfu/g, bacterium time-to-live 〉=12 month.
Description
Technical field
The present invention relates to a kind of preparation method of milk powder, particularly a kind of preparation method of rhamnose lactobacillus milk powder.
Background technology
In numerous lactobacillus of human, Lactobacillus rhamnosus (Lactolacillus rhamnosus) is one of present internationally recognized probiotics, show that by a large amount of basic research and application study result it has a lot of merits and important physical function.For example, it has the performance of sticking, and the ability of its stomach juice-resistant and anti-bile is better than the common sour milk bacterial classification; Have the field planting resistance, can suppress growth and the breeding of harmful bacterium; Can reduce or eliminate vivotoxin and carcinogen; Can protect gastrointestinal mucosa; The prevention constipation; Prevention and treatment diarrhoea; Control allergic reaction etc.Therefore, the Lactobacillus rhamnosus product is noticeable in the world.Nineteen ninety Finland dimension Aktiebolaget Leo (SE) Box 941, S-251 09 Helsingborg, Sweden (Valio Ld) has released Lactobacillus rhamnosus GG product.Lactobacillus rhamnosus GG product mainly contains fermented dairy product, preserving milk, cheese, baby food, fruit juice, beverage and medicine at present.At present existing more than 30 countries and regions, the whole world at production and selling by the product of Lactobacillus rhamnosus GG fermentation or contain the product of Lactobacillus rhamnosus GG.But still do not have the rhamnose lactobacillus milk powder product, do not adopt the spray drying device continuous batch of ordinary powdered milk production to produce the report of this product yet.
Summary of the invention
The object of the present invention is to provide a kind ofly except whole nutrition and function, also contain the Lactobacillus rhamnosus to the human body beneficial of a large amount of work with ordinary powdered milk, obvious health-care efficacy, storing is convenient, the preparation method of the rhamnose lactobacillus milk powder of instant.
For achieving the above object, the technical solution used in the present invention is:
1) sterilization with concentrate: behind 135～150 ℃, 1～2 second high-temperature instantaneous sterilization, is 17.33 * 10 in vacuum with raw material cow's milk under 45～50 ℃
3～16.00 * 10
3Decompression concentrates in the vacuum concentration pot of handkerchief, obtains milk solids content and be 40～50% concentrated breast;
2) actication of culture: with raw material cow's milk behind 135～150 ℃, 1～2 second high-temperature instantaneous sterilization, be cooled to 35～40 ℃, inoculate Lactobacillus rhamnosus then, inoculum concentration is 1%～3% of a raw milk weight, and the jerusalem artichoke powder of interpolation raw milk weight 0.1～0.5%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, obtain activated spawn;
3) homogeneous: the activated spawn that will concentrate newborn weight 10%～30% makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 20～25MPa and 45～50 ℃;
4) spray-drying: with the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
Because the present invention adopts the jerusalem artichoke powder to increase microbial inoculum as Lactobacillus rhamnosus, broken through (EAT 130-160 ℃ of high temperature spray-drying technology, temperature of outgoing air 70-75 ℃) the middle low guardian technique difficult problem of Lactobacillus rhamnosus survival rate, Lactobacillus rhamnosus viable count 〉=1 * 10 in the finished product milk powder that makes
7Cfu/g, bacterium time-to-live 〉=12 month.
The specific embodiment
Embodiment 1: at first with raw material cow's milk behind 140 ℃, 1 second high-temperature instantaneous sterilization, under 50 ℃, be 17.33 * 10 in vacuum
3Decompression concentrates and to obtain concentrating breast in the vacuum concentration pot of handkerchief, measures down that to concentrate newborn concentration be 12～16B ' e for 50 ℃, and milk solids content is 40～50%; Secondly with raw material cow's milk behind 150 ℃, 1 second high-temperature instantaneous sterilization, be cooled to 40 ℃, inoculate Lactobacillus rhamnosus (Lactolacillus rhamnosus 1.26 or 1.552 then, provide by Chinese common micro-organisms culture presevation administrative center), inoculum concentration is 3% of a raw milk weight, and adds the jerusalem artichoke powder of raw milk weight 0.3%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn; The activated spawn that will concentrate newborn weight 15% then makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 25MPa and 47 ℃; With the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder at last, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
Embodiment 2: at first with raw material cow's milk behind 135 ℃, 2 seconds high-temperature instantaneous sterilization, under 48 ℃, be 17.10 * 10 in vacuum
3Decompression concentrates and to obtain concentrating breast in the vacuum concentration pot of handkerchief, measures down that to concentrate newborn concentration be 12～16B ' e for 50 ℃, and milk solids content is 40～50%; Secondly with raw material cow's milk behind 140 ℃, 2 seconds high-temperature instantaneous sterilization, be cooled to 38 ℃, inoculate Lactobacillus rhamnosus (Lactolacillus rhamnosus 1.26 or 1.552 then, provide by Chinese common micro-organisms culture presevation administrative center), inoculum concentration is 2% of a raw milk weight, and adds the jerusalem artichoke powder of raw milk weight 0.5%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn; The activated spawn that will concentrate newborn weight 20% then makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 22MPa and 45 ℃; With the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder at last, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
Embodiment 3: at first with raw material cow's milk behind 150 ℃, 2 seconds high-temperature instantaneous sterilization, under 46 ℃, be 16.00 * 10 in vacuum
3Decompression concentrates and to obtain concentrating breast in the vacuum concentration pot of handkerchief, measures down that to concentrate newborn concentration be 12～16B ' e for 50 ℃, and milk solids content is 40～50%; Secondly with raw material cow's milk behind 135 ℃, 1 second high-temperature instantaneous sterilization, be cooled to 36 ℃, inoculate Lactobacillus rhamnosus (Lactolacillus rhamnosus 1.26 or 1.552 then, provide by Chinese common micro-organisms culture presevation administrative center), inoculum concentration is 1% of a raw milk weight, and adds the jerusalem artichoke powder of raw milk weight 0.1%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn; The activated spawn that will concentrate newborn weight 10% then makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 20MPa and 50 ℃; With the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder at last, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
Embodiment 4: at first with raw material cow's milk behind 135 ℃, 1 second high-temperature instantaneous sterilization, under 45 ℃, be 16.58 * 10 in vacuum
3Decompression concentrates and to obtain concentrating breast in the vacuum concentration pot of handkerchief, measures down that to concentrate newborn concentration be 12～16B ' e for 50 ℃, and milk solids content is 40～50%; Secondly with raw material cow's milk behind 140 ℃, 2 seconds high-temperature instantaneous sterilization, be cooled to 35 ℃, inoculate Lactobacillus rhamnosus (Lactolacillus rhamnosus 1.26 or 1.552 then, provide by Chinese common micro-organisms culture presevation administrative center), inoculum concentration is 1% of a raw milk weight, and adds the jerusalem artichoke powder of raw milk weight 0.4%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn; The activated spawn that will concentrate newborn weight 30% then makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 24MPa and 48 ℃; With the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder at last, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
Claims (5)
1, the preparation method of rhamnose lactobacillus milk powder is characterized in that:
1) sterilization with concentrate: behind 135～150 ℃, 1～2 second high-temperature instantaneous sterilization, is 17.33 * 10 in vacuum with raw material cow's milk under 45～50 ℃
3～16.00 * 10
3Decompression concentrates in the vacuum concentration pot of handkerchief, obtains milk solids content and be 40～50% concentrated breast;
2) actication of culture: with raw material cow's milk behind 135～150 ℃, 1～2 second high-temperature instantaneous sterilization, be cooled to 35～40 ℃, inoculate Lactobacillus rhamnosus then, inoculum concentration is 1%～3% of a raw milk weight, and the jerusalem artichoke powder of interpolation raw milk weight 0.1～0.5%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, obtain activated spawn;
3) homogeneous: the activated spawn that will concentrate newborn weight 10%～30% makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 20～25MPa and 45～50 ℃;
4) spray-drying: with the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
2, the preparation method of rhamnose lactobacillus milk powder according to claim 1 is characterized in that:
1) sterilization with concentrate: behind 140 ℃, 1 second high-temperature instantaneous sterilization, is 17.33 * 10 in vacuum with raw material cow's milk under 50 ℃
3To concentrate and to obtain milk solids content be 40～50% concentrated breast in decompression in the vacuum concentration pot of handkerchief;
2) actication of culture: with raw material cow's milk behind 150 ℃, 1 second high-temperature instantaneous sterilization, be cooled to 40 ℃, inoculate Lactobacillus rhamnosus then, inoculum concentration is 3% of a raw milk weight, and the jerusalem artichoke powder of interpolation raw milk weight 0.3%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn;
3) homogeneous: the activated spawn that will concentrate newborn weight 15% makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 25MPa and 47 ℃;
4) spray-drying: with the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
3, the preparation method of rhamnose lactobacillus milk powder according to claim 1 is characterized in that:
1) sterilization with concentrate: at first with raw material cow's milk behind 135 ℃, 2 seconds high-temperature instantaneous sterilization, under 48 ℃, be 17.10 * 10 in vacuum
3To concentrate and to obtain milk solids content be 40～50% concentrated breast in decompression in the vacuum concentration pot of handkerchief;
2) actication of culture: with raw material cow's milk behind 140 ℃, 2 seconds high-temperature instantaneous sterilization, be cooled to 38 ℃, inoculate Lactobacillus rhamnosus then, inoculum concentration is 2% of a raw milk weight, and the jerusalem artichoke powder of interpolation raw milk weight 0.5%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn;
3) homogeneous: the activated spawn that will concentrate newborn weight 20% makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 22MPa and 45 ℃;
4) spray-drying: with the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
4, the preparation method of rhamnose lactobacillus milk powder according to claim 1 is characterized in that:
1) sterilization with concentrate: behind 150 ℃, 2 seconds high-temperature instantaneous sterilization, is 16.00 * 10 in vacuum with raw material cow's milk under 46 ℃
3To concentrate and to obtain milk solids content be 40～50% concentrated breast in decompression in the vacuum concentration pot of handkerchief;
2) actication of culture: with raw material cow's milk behind 135 ℃, 1 second high-temperature instantaneous sterilization, be cooled to 36 ℃, inoculate Lactobacillus rhamnosus then, inoculum concentration is 1% of a raw milk weight, and the jerusalem artichoke powder of interpolation raw milk weight 0.1%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn;
3) homogeneous: the activated spawn that will concentrate newborn weight 10% makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 20MPa and 50 ℃;
4) spray-drying: with the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
5, the preparation method of rhamnose lactobacillus milk powder according to claim 1 is characterized in that:
1) sterilization with concentrate: at first with raw material cow's milk behind 135 ℃, 1 second high-temperature instantaneous sterilization, under 45 ℃, be 16.58 * 10 in vacuum
3To concentrate and to obtain milk solids content be 40～50% concentrated breast in decompression in the vacuum concentration pot of handkerchief;
2) actication of culture: with raw material cow's milk behind 140 ℃, 2 seconds high-temperature instantaneous sterilization, be cooled to 35 ℃, inoculate Lactobacillus rhamnosus then, inoculum concentration is 1% of a raw milk weight, and the jerusalem artichoke powder of interpolation raw milk weight 0.4%, then 30 ℃ of following heat insulating culture, when being cultured to acidity and reaching 50～60 ° of T, stop cultivating and obtain activated spawn;
3) homogeneous: the activated spawn that will concentrate newborn weight 30% makes an addition to concentrated Ruzhong, carries out homogeneous and obtains concentrating newborn mixture, and homogenization pressure and temperature are respectively 24MPa and 48 ℃;
4) spray-drying: with the concentrated newborn mixture behind the homogeneous, carry out spray-drying and promptly get rhamnose lactobacillus milk powder, the spray-drying condition is controlled to be: EAT is 130～160 ℃, and the tower temperature is 75 ℃, and temperature of outgoing air is 70～75 ℃.
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| CN103156098A (en) * | 2011-12-13 | 2013-06-19 | 中国科学院烟台海岸带研究所 | Synbiotic food additive and preparation method thereof |
| CN103081997A (en) * | 2013-03-07 | 2013-05-08 | 贵州神奇投资有限公司 | Nutritional yoghourt and preparation method thereof |
| CN103141722B (en) * | 2013-03-13 | 2014-04-02 | 河北农业大学 | Preparation method of lactobacillus rhamnosus soybean milk direct vat set starter |
| CN107853396B (en) * | 2017-10-17 | 2020-12-01 | 陕西科技大学 | Method for preparing goat milk active beverage by using lactobacillus rhamnosus protease |
| CN111528283B (en) * | 2020-05-08 | 2023-06-13 | 君乐宝乳业集团有限公司 | Application of lactobacillus rhamnosus X253 with anti-fatigue effect and capability of improving body fatigue tolerance |
| CN112167339A (en) * | 2020-10-16 | 2021-01-05 | 光明乳业股份有限公司 | Lactobacillus rhamnosus fermented milk and preparation method thereof |
| CN115176841A (en) * | 2022-06-27 | 2022-10-14 | 重庆第二师范学院 | Preparation method and device of composite lactobacillus plantarum preparation with blood sugar reducing function |
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