CN100560513C - Utilize glutamic acid fermentation waste water to produce the method for biological flocculant - Google Patents
Utilize glutamic acid fermentation waste water to produce the method for biological flocculant Download PDFInfo
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- CN100560513C CN100560513C CNB2006100103681A CN200610010368A CN100560513C CN 100560513 C CN100560513 C CN 100560513C CN B2006100103681 A CNB2006100103681 A CN B2006100103681A CN 200610010368 A CN200610010368 A CN 200610010368A CN 100560513 C CN100560513 C CN 100560513C
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- glutamic acid
- waste water
- biological flocculant
- acid fermentation
- flocculant
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Abstract
Utilize glutamic acid fermentation waste water to produce the method for biological flocculant, relate to a kind of zymotechnique of flocculation agent.Thoroughly eliminate river pollution in order to make China, replacement is the synthetic polymeric flocculant of raw material with the oil, the present invention adopts compound biological flocculant two-section type fermentation process to prepare biological flocculant, when its core is to prepare nutrient solution, in nutrient solution, add glutamic acid fermentation waste water, until medium pH value=7.0～7.5.By the method for the invention, utilize agricultural wastes straw and glutamate production glutamic acid wastewater " with useless pollution treatment ", not only can promote agricultural and industrial production to circulate benignly, and can reduce the negative impact that burning straw and discharge of wastewater bring to environment.
Description
Technical field
The present invention relates to a kind of zymotechnique of flocculation agent.
Background technology
Biological flocculant (Bioflocculant, be called for short BF) be that a class is by biogenic, mainly get by microbial fermentation, can make cohesion, the sedimentary special polymer substances such as solid suspended particle, somatic cells and colloidal particle that are difficult for degraded in the liquid, utilize biotechnology, through separating, extracting the novel flocculant that obtains, have biological degradability and security.It is a kind of water conditioner of novel, efficient, nontoxic, non-secondary pollution, and human beings'health and environment protection are all had very important practical sense.Biological flocculant is typical green water conditioner, and it adapts to the requirement of contemporary Sustainable development, has represented one of important directions of flocculation agent research and development.
Compound biological flocculant (Compound bioflocculant, be called for short CBF) be to be fermentation raw material with agricultural wastes straw fibrid element, the compound biological flocculant that utilizes cellulose degradation flora and flcos producing bacteria flora to form produces the bacterium flora, carry out the two-section type fermentation, realize that saccharification of cellulose section and bacterium for producing flocculant produce the coupling of wadding section, produce the efficient compound biological flocculant.The applicant has proposed a cover and has had the complete process route of independent intellectual property right, and applied for national patent, referring to ZL200410043861.4 on the basis of abundant investigation and feasibility study.The development and application of compound biological flocculant, the theory of the flocculation agent that will certainly break traditions promotes the development of environmental organism preparation technique, makes the environmental organism preparation move towards diversification more.
Glutamic acid fermentation waste water is a high concentrated organic wastewater, has characteristics such as acid strong, high COD, high BOD, high sulfate radical, high thalline content, low temperature, and improvement work is difficulty.Contain L-glutamic acid, reducing sugar, SS and ammonia nitrogen in the waste water, precious resources has not only been wasted in its any discharging, and causes serious environmental to pollute.
Summary of the invention
The purpose of this invention is to provide a kind of is raw material with agricultural crop straw and glutamic acid fermentation organic waste water; utilize biotechnology; the compound microbial flocculation agent of the high effect nontoxic of production market active demand; this product is widely used in feedwater, draining and sewage disposal; make China thoroughly eliminate river pollution; replacement is the synthetic polymeric flocculant of raw material with the oil; recycle and environment protection and enterprise's organic waste water recycling treatment for China's renewable resources reduce production costs and make major contribution.
The method of utilizing glutamic acid fermentation waste water to produce biological flocculant of the present invention adopts compound biological flocculant two-section type fermentation process to prepare biological flocculant, when its core is to prepare nutrient solution, in nutrient solution, add glutamic acid fermentation waste water, until medium pH value=7.0～7.5.
It is a hard nut to crack that fermentation industry waste water, particularly monosodium glutamate industry glutamic acid fermentation waste water are handled always.The organic waste water that the present invention utilizes fermentation industry is used for the preparation of compound biological flocculant as cheap nitrogenous source, has both handled organic waste water, also is used as the process water and the part nutrition base of biological flocculant simultaneously.Technological innovation of the present invention embodies a concentrated reflection of " 3 compound " and " 2 resource utilizations "." 3 compound " refers to that raw materials for production are compound, effective constituent is compound and throwing out mechanism is compound." 2 resource utilizations " refers to plain resource utilization of agricultural wastes straw fibrid and monosodium glutamate industry glutamic acid fermentation waste water resource utilization.By the method for the invention, utilize agricultural wastes straw and glutamate production glutamic acid wastewater " with useless pollution treatment ", not only can promote agricultural and industrial production to circulate benignly, and can reduce the negative impact that burning straw and discharge of wastewater bring to environment.Have following technical characterstic:
(1) pH=8.0～10.0 of the stalk handled thing that obtains through pre-treatment, and the pH=3.0 of glutamic acid fermentation waste water～6.0, the two mixes the main ingredient as fermentation substrate, need not use soda acid to carry out the adjusting of pH value, and effectively reduce sulfate concentration, reduce operation steps, realize that fermentation costs reduces.
(2) remaining L-glutamic acid composition is used as organic nitrogen source in the glutamic acid fermentation waste water, and it is original 1/4～1/5 that the addition of yeast extract paste when preparing burden for the second time is reduced to, and turns waste into wealth, and realizes that fermentation costs reduces.
(3) remaining thalline participates in the Production Flow Chart of compound biological flocculant in the glutamic acid fermentation waste water, has certain throwing out after the self-dissolving, does not need to separate and remove step, can be directly as the medicament composition of compound biological flocculant." plyability " of compound biological flocculant effective ingredient makes that its flocculating property has efficiently, wide spectrum and economic characteristics.
Description of drawings
Fig. 1 is the process flow sheet of embodiment two.
Embodiment
Embodiment one: present embodiment utilizes glutamic acid fermentation waste water to produce biological flocculant according to following method: a, employing stalk cellulose biolobic material material are starting material, use the method for physical-chemical coupling to carry out pre-treatment, to improve the cellulosic degradation rate of follow-up fermentation stage, that is: 1. earlier with the raw material mechanical disintegration, 2. again with taking off the wooden chemistry agent treated, 3. use steamed quick-fried again after handling, further break the crystalline texture and the protective layer of cellulosic molecule, make material become fluffy; B, add glutamic acid fermentation waste water, the preparation nutrient solution, make its pH value to 7.0～7.5 after, to wherein adding inorganic salt: 0.5～5g/L KH
2PO
4, 0.1～1g/L MgSO
47H
2O, 0.1g/L NaCl, 0.01～0.05g/L FeCl
3, 1.0～5g/L NaNO
3, to satisfy the nutritional requirement of microbial fermentation, wherein raw material accounts for 1～5% of nutrient solution mass volume ratio, and is standby through sterilising treatment; C, first section fermentation unit are the saccharification of cellulose section, in above-mentioned nutrient solution, add cellulose-degrading bacteria, with cellulose conversion is glucose and the cellobiose that bacterium is easy to utilize, finish cellulosic efficiently saccharifying process, control connects that bacterium amount is 2～6%, leavening temperature is that 30～50 ℃, fermentation time are that 70～80h, air demand are 60～80%, rotating speed is 300～350r/min, finishes first section fermentation; D, second section fermentation unit are that bacterium for producing flocculant produces the wadding section, promptly utilize saccharification liquid to produce the biological flocculant section, after first section fermentation ends, add bacterium for producing flocculant, generate compound biological flocculant, control connects that the bacterium amount is 4～6%, leavening temperature is that 25～40 ℃, fermentation time are that 24～30h, air demand are 50～70%, rotating speed is 300～350r/mm.
In the present embodiment, described inorganic salt are made up of following compositions: 0.5～5g/L KH
2PO
4, 0.1～1g/LMgSO
47H
2O, 0.1g/L NaCl, 0.01～0.05g/L FeCl
3, 1.0～5g/L NaNO
3Described cellulose-degrading bacteria is Alcaligenes xylosoxidans Achromobacter xylosoxidans sp. or Alcaligenes faecalis Alcaligenes faecalis sp.; Described bacterium for producing flocculant is micrococcus sp Micrococcus sp. or bacillus Bacillus sp..
Embodiment two: present embodiment is an example with certain Gourmet Powder Factory's glutamic acid fermentation waste water, wherein the process drain water-quality COD
Cr=2500mg/L, BOD
5=1200mg/L, SS≤300mg/L, TN≤200mg/L, pH=5.0～6.0; The water of condensation water-quality COD
Cr=8000mg/L, BOD
5=400mg/L, SS≤2000mg/L, TN≤300mg/L, pH=3.0～5.0, the two water yield is identical.Process drain and water of condensation mix, behind the quiet heavy 4h, add stalk material handling and inorganic salt according to the certain mass volume ratio, make its pH=7.0～7.5, specific embodiment as shown in Figure 1, treatment effect is as follows:
(1) pH=8.0～10.0 of the stalk handled thing that obtains through pre-treatment, and the pH=3.0 of glutamic acid fermentation waste water～6.0, the two mixes the main ingredient as fermentation substrate, need not use soda acid to carry out the adjusting of pH value or consumption and be reduced to original 5%～8%.
(2) remaining L-glutamic acid composition is used as organic nitrogen source in the glutamic acid fermentation waste water, makes the addition of yeast extract paste when preparing burden for the second time be reduced to original 1/4～1/5.
(3) glutamic acid fermentation waste water is after present method is used, and the sewage that is produced has descended 60%～80% than glutamic acid fermentation waste water COD, and pH is 7.0～7.5, helps sewage disposal thereafter, has the effect of contamination-reducing synergistic.
Claims (1)
1, utilize glutamic acid fermentation waste water to produce the method for biological flocculant, described method adopts compound biological flocculant two-section type fermentation process to prepare biological flocculant, when it is characterized in that preparing nutrient solution, in nutrient solution, add glutamic acid fermentation waste water, until medium pH value=7.0～7.5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100103681A CN100560513C (en) | 2006-08-04 | 2006-08-04 | Utilize glutamic acid fermentation waste water to produce the method for biological flocculant |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100103681A CN100560513C (en) | 2006-08-04 | 2006-08-04 | Utilize glutamic acid fermentation waste water to produce the method for biological flocculant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1911824A CN1911824A (en) | 2007-02-14 |
| CN100560513C true CN100560513C (en) | 2009-11-18 |
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| CNB2006100103681A Expired - Fee Related CN100560513C (en) | 2006-08-04 | 2006-08-04 | Utilize glutamic acid fermentation waste water to produce the method for biological flocculant |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102776239B (en) * | 2012-07-25 | 2014-05-07 | 华南理工大学 | Microbial flocculant produced by yeast wastewater and beer wastewater and method |
| CN108911013B (en) * | 2018-08-17 | 2021-01-19 | 浙江蜜蜂集团有限公司 | Method for treating glutamic acid fermentation wastewater |
| CN110015760A (en) * | 2019-04-08 | 2019-07-16 | 齐鲁工业大学 | The method for producing biological flocculant using stalk and glutamic acid fermentation waste water |
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Granted publication date: 20091118 Termination date: 20110804 |