CN100542677C - The continuous production equipment of biochemical product magnetic adsorption and desorption - Google Patents
The continuous production equipment of biochemical product magnetic adsorption and desorption Download PDFInfo
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- CN100542677C CN100542677C CNB2006101137789A CN200610113778A CN100542677C CN 100542677 C CN100542677 C CN 100542677C CN B2006101137789 A CNB2006101137789 A CN B2006101137789A CN 200610113778 A CN200610113778 A CN 200610113778A CN 100542677 C CN100542677 C CN 100542677C
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Abstract
The invention belongs to the adsorption separation technology field of biogenetic products, particularly the continuous production equipment of biochemical product magnetic adsorption and desorption.The present invention passes through gas-lifting type adsorption tower (tower 1), gas-lifting type desorber (tower 2) and storage tank combine, two tower bottoms are all settled distribution grid, the mode that adopts bubble to disperse to stir realizes fully mixing of microballoon and material liquid, be connected with very thin rectangular tube between tower 1 and tower 2 and tower 2 and the storage tank, be used to carry magnetic microsphere, be provided with the recycling that return line is used for magnetic microsphere between storage tank and the tower 1.The Magnetic Isolation equipment that is connected two tower exits is made of the group of magnets of crawler belt shape, and this group of magnets drives at this by motor and sentence certain speed rotation, and magnetic microsphere can be obtained enrichment and separate under the effect of this rotary magnet.
Description
Technical field
The invention belongs to the adsorption separation technology field of biogenetic products, particularly the continuous production equipment of biochemical product magnetic adsorption and desorption.
Technical background
The superparamagnetism microballoon has the advantage that general solid particle does not possess: at first, it has superparamagnetism, present good magnetic under the magnetic field condition adding, after externally-applied magnetic field removes, the remanent magnetism of this magnetic microsphere carrier is zero, utilize this character can be easily to its position, separation, reclaimer operation; Secondly, can connect different functional groups and can give magnetic microsphere different biological selectivities, and then can carry out the separation of biological products such as natural products, protein, nucleic acid, cell as required to its graft modification on the magnetic microsphere surface; In addition, this magnetic microsphere has the advantage that particle is little, specific area is big and consumption is few, when carrying out operation such as adsorption-desorption or Reaction Separation, has also reduced raw materials consumption when unit operations speed is accelerated greatly, has therefore improved production efficiency.
The adsorbing separation of traditional biogenetic products is divided into two of absorption (reaction) and desorbs (separation) unit operations independently separately usually.For absorption (reaction) unit operations, used to be in reactor, to adopt mechanical agitation or electromagnetic agitation that magnetic-particle is adsorbed in reactor or physical-chemical reaction, in order to prevent that churned mechanically tangential mixing power from smashing microbial cell or destroying biogenetic products, adopt to contain O now
2Mode Deng the gas bubbling replaces mechanical agitation, to reach production application.For the unit operations of desorb (separation), be that product is obtained, absorption and desorb two unit operations all will separate magnetic microsphere, and traditional handicraft often adopts filtration to wait the intermittent operation mode, and production efficiency is lower, troublesome poeration.
Existing magnetic separation technique and equipment adopt electromagnetic field usually, and its magnetic field intensity is weak, cost of equipment is high.Now adopt the major part of permanent magnet, not only can cut down the consumption of energy, reduce production costs, can also improve magnetic field intensity, enhance productivity as Magnetic Isolation equipment.
Report about magnetic reaction unit and Magnetic Isolation device has a lot, as patent CN:86104692 related only be a magnetic roller separation device, will not react with separate or adsorption and desorption integrated, though as patent CN:02120810 design is the integrated reaction and separation device, but the dispersion effect of reactor part is not obvious in the device, the present invention not only will react with separate or adsorption and desorption operation integrated, also adopted the mode of bubbling in the biochemical reactor to carry out dispersion in the tower.This invention integrates the dispersion technology of bioreactor and magnetic separation technique and unit operations integrated technique, and help continued operation, scale and amplify, reduce cost and improve the product yield productive rate, be one of important directions of present chemical industry development.
Summary of the invention
The continuous production equipment that the purpose of this invention is to provide the biochemical product magnetic adsorption and desorption.
The continuous production equipment of biochemical product magnetic adsorption and desorption of the present invention comprises gas-lifting type absorption (reaction) tower (tower 1), gas-lifting type desorb (separation) tower (tower 2), distribution grid, group of magnets, storage tank etc. as shown in Figure 1;
One gas-lifting type absorption (reaction) tower, have the import and the air inlet of material liquid in the bottom of tower, the distribution grid that is used for material liquid and bubble dispersion mixing is housed in the bottom of tower the inside, bottom, side at tower, and above distribution grid, have the inlet of magnetic microsphere, in this porch the pipeline that is connected with storage tank is installed, is used for recycling of magnetic microsphere; Top at tower has the gas outlet, is used for the balance tank pressure; Side on the top of tower has material liquid outlet, the pipeline that has two outlets in exit connection one, one group of magnets that is connected on the cast crawler belt is installed at the pipeline place, be connected group of magnets on the cast crawler belt by the motor driven rotary, the outlet of one pipeline is used to derive waste liquid, and another outlet connects a pipeline (magnetic microsphere that will adsorb product imports next tower) that is connected with gas-lifting type desorb (separation) tower (tower 2);
One gas-lifting type desorb (separation) tower, have the import and the air inlet of eluant, eluent in the bottom of tower, the distribution grid that is used for eluant, eluent and bubble dispersion mixing is housed in the bottom of tower the inside, in the bottom, side of tower, and the pipeline that is connected with gas-lifting type absorption (reaction) tower (magnetic microsphere that has adsorbed product enter the mouth thus importing) is installed above distribution grid; Top at tower has the gas outlet, is used for the balance tank pressure; Side on the top of tower has material liquid outlet, the pipeline that has two outlets in exit connection one, one group of magnets that is connected on the cast crawler belt is installed at the pipeline place, be connected group of magnets on the cast crawler belt by the motor driven rotary, the outlet of one pipeline is used to derive the product after concentrating, another outlet connects a pipeline that is connected with storage tank (being used for magnetic microsphere reclaims), with desorb the magnetic microsphere importing of product;
One pipeline is connected storage tank with gas-lifting type absorption (reaction) tower, the magnetic microsphere after will reclaiming under the effect of calming the anger imports the bottom of gas-lifting type absorption (reaction) tower.
Described gas-lifting type desorb (separation) tower is little more a lot of than the capacity of gas-lifting type absorption (reaction) tower, by schematic diagram 1 also as can be seen, this is because be the absorption of carrying out product in the material liquid in the tower 1, in the tower 2 desorb of carrying out product on the magnetic microsphere, the concentration process that its relative tower 1 is a product.
The described magnet that is connected on the crawler belt is permanent magnet, and the N of its whole magnet or the S utmost point are outside crawler belt; Described crawler belt direction of rotation is magnetic microsphere effluent gases lift-type absorption (reaction) tower or gas-lifting type desorb (separation) tower direction.This group of magnets deposits the magnetic microsphere in the pipeline get off, and, reach the pipeline of connection gas-lifting type desorb (separation) tower and storage tank and obtain enrichment along with pipeline flows to connection gas-lifting type absorption (reaction) tower on group of magnets next door and the pipeline of gas-lifting type desorb (separation) tower.
The material of described tower, storage tank and pipeline is stainless steel, glass or plastics.
The present invention as shown in Figure 1, material liquid and gas are imported by tower 1 bottom together, in tower, fully mixing after the process distribution grid disperses with from the active magnetic microsphere of the process desorb recovery of tower bottom side inflow, the product that needs in the material liquid are reclaimed adsorbs (reaction), the magnetic microsphere coalescence under the magneticaction of rotary magnet group that has adsorbed product, a tubule by the rotary magnet below imports tower 2 bottoms, and the material liquid (waste liquid) that has been adsorbed product is discharged by the extra heavy pipe in top.The magnetic microsphere that has adsorbed product enters after the tower 2, under the effect of bubble, fully mix and interact and carry out wash-out with eluant, eluent, the product that adsorbs on the magnetic microsphere is eluted outside products export is discharged tower, desorb the magnetic microsphere of product under the effect of rotary magnet group, collect by enriching and recovering to storage tank by a tubule, the bottom that is transported to tower 1 under the effect of calming the anger recycles.
The present invention passes through the gas-lifting type adsorption tower, gas-lifting type desorber and storage tank combine, two tower bottoms are all settled distribution grid, the mode that adopts bubble to disperse to stir realizes fully mixing of microballoon and material liquid, be connected with very thin rectangular tube between tower 1 and tower 2 and tower 2 and the storage tank, be used to carry magnetic microsphere, be provided with the recycling that return line is used for magnetic microsphere between storage tank and the tower 1.The Magnetic Isolation equipment that is connected two tower exits is made of the group of magnets of crawler belt shape, and this group of magnets drives at this by motor and sentence certain speed rotation, and magnetic microsphere can be obtained enrichment and separate under the effect of this rotary magnet.
Description of drawings
Fig. 1. the schematic diagram of present device.
Fig. 2. the magnetic carrier that the embodiment of the invention 1 experiment obtains is to bovine serum albumin(BSA) (BSA) adsorbance and desorption quantity (under the 1M NaCl condition) curve map over time.
Reference numeral
1,4. distribution grid 2. material liquid liquid levels 3. eluant, eluent liquid levels
5,6. group of magnets 7. storage tanks
8. adsorbed product magnetic microsphere 9. desorbs the magnetic microsphere of product
The specific embodiment
Embodiment 1.
See also Fig. 1.One gas-lifting type adsorption tower (tower 1), have the import and the air inlet of material liquid in the bottom of tower, the distribution grid 1 that is used for material liquid 2 and bubble dispersion mixing is housed in the bottom of tower the inside, bottom, side at tower, and above distribution grid, have the inlet of magnetic microsphere, in this porch the pipeline that is connected with storage tank is installed, is used for recycling of magnetic microsphere; Top at tower has the gas outlet, is used for the balance tank pressure; Side on the top of tower has material liquid outlet, the pipeline that has two outlets in exit connection one, one group of magnets 5 that is connected on the cast crawler belt is installed at the pipeline place, be connected group of magnets on the cast crawler belt by the motor driven rotary, one outlet that has the pipeline of valve is used to derive waste liquid, another outlet connects a pipeline that is connected with gas-lifting type desorb (separation) tower (tower 2), and this pipeline will adsorb the next tower of magnetic microsphere 8 importings of product, i.e. gas-lifting type desorb (separation) tower;
One gas-lifting type desorb (separation) tower (tower 2), have the import and the air inlet of eluant, eluent in the bottom of tower, the distribution grid 4 that is used for eluant, eluent 3 and bubble dispersion mixing is housed in the bottom of tower the inside, in the bottom, side of tower, and the pipeline that is connected with gas-lifting type absorption (reaction) tower (magnetic microsphere that has adsorbed product enter the mouth thus importing) is installed above distribution grid; Top at tower has the gas outlet, is used for the balance tank pressure; Side on the top of tower has material liquid outlet, the pipeline that has two outlets in exit connection one, one group of magnets 6 that is connected on the cast crawler belt is installed at the pipeline place, be connected group of magnets on the cast crawler belt by the motor driven rotary, one outlet that has the pipeline of valve is used to derive the product after concentrating, another outlet connects a pipeline that is connected with storage tank 7 (being used for magnetic microsphere reclaims), with desorb magnetic microsphere 9 importings of product;
One pipeline is connected storage tank with gas-lifting type absorption (reaction) tower, the magnetic microsphere after will reclaiming under the effect of calming the anger imports the bottom of gas-lifting type absorption (reaction) tower.
1. this magnetic microsphere is to be prepared from (PMMA-PEG-CB) with the active triasine dyes of magnetic polymethylacrylic acid macrogol ester microsphere surface grafting, experimental results show that this magnetic microsphere has superparamagnetism.
2. the experimental technique of affine adsorption and desorption: as the affine absorption bovine serum albumin(BSA) of carrier (BSA), under high salt concn the bovine serum albumin(BSA) on the carrier (BSA) desorb is got off again with the active triasine dyes magnetic microsphere of polymethylacrylic acid macrogol ester (PMMA-PEG-CB).
(1) adsorption operations:
This magnetic microsphere is affine separation bovine serum albumin(BSA) (BSA) all operations carries out in pH=6, concentration are the Michaelis phosphate buffer of 20mM.The 20ml phosphate buffer that will contain this magnetic microsphere of 160mg adds in the stirrer mixer, stirred for several minute, other adds bovine serum albumin(BSA) (BSA) concentration is the phosphate buffer 20ml of 2.0mg/ml, making bovine serum albumin(BSA) (BSA) total concentration is 1.0mg/ml, meanwhile, start the delicate metering infusion pump, the control liquid inventory is about 1ml/min, analyze bovine serum albumin(BSA) (BSA) concentration at magnetizing mediums separator tube exit timing sampling, calculate adsorbance over time.
(2) desorb operation:
The desorption kinetic curve of bovine serum albumin(BSA) on this magnetic microsphere (BSA) carries out in NaCl concentration is the phosphate buffer of 1.0M with same measuring method.
3. curve is as shown in Figure 2 over time to bovine serum albumin(BSA) (BSA) adsorbance and desorption quantity (under the 1M NaCl condition) for the magnetic microsphere that obtains of experiment.
Experiment conclusion: this superparamagnetism is affine separation microballoon, at low salt concn bovine serum albumin(BSA) (BSA) had higher affine adsorption capacity, improve salinity most of bovine serum albumin(BSA) (BSA) desorb is got off, absorption and desorb reach balance in 10-20min.
Claims (5)
1. the continuous production equipment of a biochemical product magnetic adsorption and desorption comprises the gas-lifting type adsorption tower, gas-lifting type desorber, distribution grid, group of magnets, storage tank; It is characterized in that:
One gas-lifting type adsorption tower, have the import and the air inlet of material liquid in the bottom of tower, the distribution grid that is used for material liquid and bubble dispersion mixing is housed in the bottom of tower the inside, bottom, side at tower, and above distribution grid, have the inlet of magnetic microsphere, first pipeline that is connected with storage tank is installed in this porch; Top at tower has the gas outlet; Side on the top of tower has material liquid outlet, second pipeline that has two outlets in exit connection one, at the second pipeline place one group of magnets that is connected on the cast crawler belt is installed, be connected group of magnets on the cast crawler belt by the motor driven rotary, wherein, one outlet of second pipeline is used to derive waste liquid, and another outlet connects the 3rd pipeline that is connected with the gas-lifting type desorber;
One gas-lifting type desorber, have the import and the air inlet of eluant, eluent in the bottom of tower, in the bottom of tower the inside the distribution grid that is used for eluant, eluent and bubble dispersion mixing is housed,, and the 3rd pipeline that is connected with the gas-lifting type adsorption tower is installed above distribution grid in the bottom, side of tower; Top at tower has the gas outlet; Side on the top of tower has material liquid outlet, the 4th pipeline that has two outlets in exit connection one, at the 4th pipeline place one group of magnets that is connected on the cast crawler belt is installed, be connected group of magnets on the cast crawler belt by the motor driven rotary, wherein, one outlet of the 4th pipeline is used to derive the product after concentrating, and another outlet connects the 5th pipeline that is connected with storage tank, with desorb the magnetic microsphere importing of product;
First pipeline is connected storage tank with the gas-lifting type adsorption tower, the magnetic microsphere after will reclaiming under the effect of calming the anger imports the bottom of gas-lifting type adsorption tower.
2. equipment according to claim 1 is characterized in that: the described magnet that is connected on the crawler belt is permanent magnet.
3. equipment according to claim 1 and 2 is characterized in that: the N of the described whole magnet that are connected the magnet on the crawler belt or the S utmost point are outside crawler belt.
4. equipment according to claim 1 and 2 is characterized in that: described crawler belt direction of rotation is magnetic microsphere effluent gases lift-type adsorption tower or gas-lifting type desorber direction.
5. equipment according to claim 3 is characterized in that: described crawler belt direction of rotation is magnetic microsphere effluent gases lift-type adsorption tower or gas-lifting type desorber direction.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101137789A CN100542677C (en) | 2006-10-16 | 2006-10-16 | The continuous production equipment of biochemical product magnetic adsorption and desorption |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006101137789A CN100542677C (en) | 2006-10-16 | 2006-10-16 | The continuous production equipment of biochemical product magnetic adsorption and desorption |
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| Publication Number | Publication Date |
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| CN101164700A CN101164700A (en) | 2008-04-23 |
| CN100542677C true CN100542677C (en) | 2009-09-23 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI362964B (en) | 2009-12-23 | 2012-05-01 | Ind Tech Res Inst | Magnetic separation device and method for separating magnetic substances in bio-samples |
| CN102527085B (en) * | 2012-02-06 | 2015-02-18 | 中国科学院过程工程研究所 | Method for extracting effective components of Chinese herbal medicine by magnetic absorbent in an auxiliary way |
| CN105087552B (en) * | 2015-09-23 | 2018-08-17 | 河南中标检测服务有限公司 | A method of extraction animal tissue nucleic acid |
| CN106694224B (en) * | 2017-01-11 | 2019-04-05 | 中国科学院过程工程研究所 | A kind of whole process serialization gas magnetic assist separation system |
| CN107986387B (en) * | 2017-12-14 | 2023-09-22 | 长安大学 | Ultrasound-assisted selective photocatalysis method and device based on magnetic molecular imprinting |
| CN111375488A (en) * | 2018-12-28 | 2020-07-07 | 苏州海狸生物医学工程有限公司 | Automatic magnetism screening plant |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990015660A1 (en) * | 1989-06-13 | 1990-12-27 | Atsunobu Sakamoto | Bag-like filter |
| CN2325115Y (en) * | 1998-05-19 | 1999-06-23 | 黄道培 | Apparatus for separating magnetic particles |
| CN1463804A (en) * | 2002-06-03 | 2003-12-31 | 中国科学院过程工程研究所 | Integrated apparatus for ultrafine magnetic grain catalytic reaction and continuous segregation in liquid |
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2006
- 2006-10-16 CN CNB2006101137789A patent/CN100542677C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1990015660A1 (en) * | 1989-06-13 | 1990-12-27 | Atsunobu Sakamoto | Bag-like filter |
| CN2325115Y (en) * | 1998-05-19 | 1999-06-23 | 黄道培 | Apparatus for separating magnetic particles |
| CN1463804A (en) * | 2002-06-03 | 2003-12-31 | 中国科学院过程工程研究所 | Integrated apparatus for ultrafine magnetic grain catalytic reaction and continuous segregation in liquid |
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| CN101164700A (en) | 2008-04-23 |
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