CN100540664C - 将突变转移到靶核酸中的方法 - Google Patents

将突变转移到靶核酸中的方法 Download PDF

Info

Publication number
CN100540664C
CN100540664C CNB038165678A CN03816567A CN100540664C CN 100540664 C CN100540664 C CN 100540664C CN B038165678 A CNB038165678 A CN B038165678A CN 03816567 A CN03816567 A CN 03816567A CN 100540664 C CN100540664 C CN 100540664C
Authority
CN
China
Prior art keywords
dna
nucleic acid
target nucleic
cells
irdna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CNB038165678A
Other languages
English (en)
Chinese (zh)
Other versions
CN1668743A (zh
Inventor
曹春渝
佐川裕章
加藤郁之进
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Takara Bio Inc
Original Assignee
Takara Bio Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Takara Bio Inc filed Critical Takara Bio Inc
Publication of CN1668743A publication Critical patent/CN1668743A/zh
Application granted granted Critical
Publication of CN100540664C publication Critical patent/CN100540664C/zh
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CNB038165678A 2002-07-12 2003-07-11 将突变转移到靶核酸中的方法 Expired - Fee Related CN100540664C (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP204887/2002 2002-07-12
JP2002204887 2002-07-12
JP2003113534 2003-04-18
JP113534/2003 2003-04-18

Publications (2)

Publication Number Publication Date
CN1668743A CN1668743A (zh) 2005-09-14
CN100540664C true CN100540664C (zh) 2009-09-16

Family

ID=30117458

Family Applications (1)

Application Number Title Priority Date Filing Date
CNB038165678A Expired - Fee Related CN100540664C (zh) 2002-07-12 2003-07-11 将突变转移到靶核酸中的方法

Country Status (10)

Country Link
US (1) US20060160219A1 (enExample)
EP (1) EP1533375B1 (enExample)
JP (1) JP4372684B2 (enExample)
CN (1) CN100540664C (enExample)
AT (1) ATE443134T1 (enExample)
AU (1) AU2003248268A1 (enExample)
CA (1) CA2492783A1 (enExample)
DE (1) DE60329302D1 (enExample)
TW (1) TW200411052A (enExample)
WO (1) WO2004007715A1 (enExample)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060189560A1 (en) * 2005-01-27 2006-08-24 Ajit Kumar Molecular method to augment RNA mediated gene silencing
US9276664B2 (en) 2007-04-30 2016-03-01 Dish Network Corporation Mobile interactive satellite services
US8457682B2 (en) 2008-03-04 2013-06-04 Dbsd Satellite Services G.P. Method and system for integrated satellite assistance services
US8626230B2 (en) 2008-03-04 2014-01-07 Dish Network Corporation Method and system for using routine driving information in mobile interactive satellite services

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2073630C (en) * 1991-08-30 2007-12-11 Atsushi Ohshima Method for synthesizing single-stranded stem-loop dnas, the products and uses therefor
WO1998040510A1 (en) * 1997-03-11 1998-09-17 Regents Of The University Of Minnesota Dna-based transposon system for the introduction of nucleic acid into dna of a cell
US6130207A (en) * 1997-11-05 2000-10-10 South Alabama Medical Science Foundation Cell-specific molecule and method for importing DNA into a nucleus
AUPP249298A0 (en) * 1998-03-20 1998-04-23 Ag-Gene Australia Limited Synthetic genes and genetic constructs comprising same I
EP1181382B1 (en) * 1999-06-01 2005-03-23 The University of Washington Recombinant adenoviral vectors expressing chimeric fiber proteins for cell specific infection and genome integration
WO2001049844A1 (en) * 1999-12-30 2001-07-12 Rutgers, The State University Of New Jersey Compositions and methods for gene silencing

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
GENERATION OF ADENOVRUS VECTORS. DIREK S STENVAERODE ET AL.JOURANL OF VIRLOLGY,Vol.73 No.11. 1999 *
NUCLEAR PROTEIN LOCALIZATION. GARCIA BUSTOS J ET AL.BIOCHINM BIOPHYS ACTA,Vol.1071 No.1. 1991 *

Also Published As

Publication number Publication date
EP1533375A4 (en) 2005-08-31
JP4372684B2 (ja) 2009-11-25
JPWO2004007715A1 (ja) 2005-11-10
ATE443134T1 (de) 2009-10-15
CA2492783A1 (en) 2004-01-22
DE60329302D1 (de) 2009-10-29
TW200411052A (en) 2004-07-01
US20060160219A1 (en) 2006-07-20
WO2004007715A1 (ja) 2004-01-22
EP1533375B1 (en) 2009-09-16
EP1533375A1 (en) 2005-05-25
AU2003248268A1 (en) 2004-02-02
TWI316958B (enExample) 2009-11-11
CN1668743A (zh) 2005-09-14

Similar Documents

Publication Publication Date Title
US10590415B2 (en) Engineered nucleic acid-targeting nucleic acids
JP3585238B2 (ja) 真核細胞における部位特異的突然変異誘発のための化合物および方法
DK3320092T3 (en) CONSTRUCTED CRISPR-CAS9 COMPOSITIONS AND METHODS OF USE
CA2382120C (en) Single-stranded oligodeoxynucleotide mutational vectors
US20160362667A1 (en) CRISPR-Cas Compositions and Methods
WO2019090174A1 (en) Novel crispr-associated transposon systems and components
WO2020210239A1 (en) Integration of nucleic acid constructs into eukaryotic cells with a transposase from oryzias
CN112513277A (zh) 使用来自螟蛾属的转座酶将核酸构建体转座入真核基因组
EP3974524A1 (en) Dna vectors, transposons and transposases for eukaryotic genome modification
CN110300802A (zh) 用于动物胚胎碱基编辑的组合物和碱基编辑方法
WO2019173248A1 (en) Engineered nucleic acid-targeting nucleic acids
CN100540664C (zh) 将突变转移到靶核酸中的方法
CN111051509A (zh) 用于电介质校准的含有c2cl核酸内切酶的组合物以及使用其进行电介质校准的方法
US20250188431A1 (en) Engineered nuclease and use thereof
KR20050021366A (ko) 표적 핵산내 돌연변이 도입 방법
WO2024191467A1 (en) Cas fusion proteins and associated methods for site specific integration
CN118599808A (zh) 新型Cas9蛋白CasC、其突变体及应用
KR20250140066A (ko) 개선된 활성을 갖는 cpf1(cas12a)의 변이체
HK40038501B (zh) 使用来自青鱂属的转座酶将核酸构建体整合入真核细胞
JP2005514928A (ja) 相同組換えによる遺伝子破壊に関して細胞を選択する迅速な方法
HK40038987B (zh) 使用来自螟蛾属的转座酶将核酸构建体转座入真核基因组
HK40038987A (en) Transposition of nucleic acid constructs into eukaryotic genomes with a transposase from amyelois

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20090916

Termination date: 20100711