CN100529077C - Wheat small GTP binding protein Rab2 gene TaRab2 and its use - Google Patents
Wheat small GTP binding protein Rab2 gene TaRab2 and its use Download PDFInfo
- Publication number
- CN100529077C CN100529077C CNB200410009930XA CN200410009930A CN100529077C CN 100529077 C CN100529077 C CN 100529077C CN B200410009930X A CNB200410009930X A CN B200410009930XA CN 200410009930 A CN200410009930 A CN 200410009930A CN 100529077 C CN100529077 C CN 100529077C
- Authority
- CN
- China
- Prior art keywords
- gene
- wheat
- binding protein
- tarab2
- rab2
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Images
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Peptides Or Proteins (AREA)
Abstract
Through screening to the wheat cDNA, the invention has acquired relative candidate EST, making use of electron extension and PT-PCR technique, it has cloned a wheat drought defying relative gene TaRab2 ( Triticum aestivum GTP-binding protein rab2). Besides, the invention relates to its application. The gene plays a dominant role in transportation of protein in eucaryotic cells, adjusts the film transporting function, effects the endocytosis of cell and transportation of synthetic protein, and can play protective role in repairing hurt of cell. If the plant is effected by the outside, the gene for resisting droughty and salt and alkali and illness is induced to be strengthened. The cloning of the gene has significant in cultivating wheat drought defying water-saving species and wheat drought defying, molecular biology research by screening method.
Description
Technical field
The present invention relates to plant genetic engineering field, more specifically, the present invention relates to the Drought-resistance in Wheat related protein gene, be i.e. the little gtp binding protein Rab2 of wheat gene TaRab2; In addition, the invention still further relates to the application of the little gtp binding protein Rab2 of wheat gene TaRab2.
Background technology
Wheat is human staple food crop, but its production development seriously is subjected to the restriction of drought and water shortage.Whole world developing country has at least 6,000 ten thousand hectares of wheat cultivations to support the arable land at rain, and yield level has only the 10%-50% under the irrigation conditions.The water requirement of 1 ton of wheat of the average every production of China is about 500-700m
3Evidence suggests that plantation resisting drought saving water kind can obviously improve the Wheat Production of arid, semiarid zone, therefore, cultivating the resisting drought saving water kind is a kind of most economical valid approach of alleviating shortage of water resources contradiction.
The improvement crop drought resistance is significant for improving agricultural productive force, is subjected to the great attention of countries in the world.Wherein, research drought resistance mechanism, clone's anti-drought gene, be an effective way by plant genetic engineering improvement crop drought resistance.Because the complicacy of drought resistance hereditary basis, the difficulty that the clone transforms anti-drought gene is bigger, still, some successful examples has been arranged.Aspect transgenosis, (Molecular Breeding.2002,10 (1-2): 71-82) the lea protein gene of wheat is imported paddy rice, improved the patience of transfer-gen plant such as Cheng to drought, salt; (Chinese ScienceBulletin.2003,48 (23): 2594-2600) change the δ-OAT gene of Arabidopis thaliana over to paddy rice and make its overexpression, analysis revealed proline content in leaf and root obviously increases WU etc., has strengthened the resistance of plant to drought and salt.Dubouzet etc. (Plant J.2003,33:751-763) paddy rice encoding transcription activator gene OsDRE1A is imported Arabidopis thaliana after, transformed plant has showed strong drought resistant, anti-salt and cold resistant property.
At present, the gene related to drought tolerance of having cloned mainly comprises following several types: (1) transcription factor: mainly be included in DREB1 A-C, DREB2 A-B, CBF1-3, Hs and the AtGluR2 etc. that find in the Arabidopis thaliana (Ceng Huazong etc. the plant genetic resources journal, 2003,4 (3): 270-273); (2) coded amino acid synthetic gene: based on proline(Pro) (Pro) synthase gene, comprise pyrroline-5-carboxylic acid synthetase gene P5CS and PVAB2, pyrroles's beautiful jade-5-carboxylate reductase gene P5CR and PproC1 and mountain spinach proline transport protein gene A hProT1 etc.; (3) functional protein gene: based on late embryo generation Abundant protein (LEA), comprise D19, B19.1D11, the D29 of cotton, the COR15a of Arabidopis thaliana and PrabaT1, the lea protein gene of wheat.Little gtp binding protein Rab2 gene has also obtained the clone in various plants, be included in the ScRab gene of cloning in the paddy rice (.Taylor ﹠amp such as ElaMizrahi-Aviv; Francis, 2002, the little gtp binding protein Rab2 gene of cloning 13:295-300) and among Sporobolusstapfianus (kind of plant in South Africa) (PatrickJ.O ' .Plant Molecular Biology such as Mahony, 1999,39:809-821) etc.
Above-mentioned little gtp binding protein is the albumen that a class plays an important role in intracellular transportation.They are in inactive state by activating in conjunction with GTP after GTP is hydrolyzed to GDP.Rab2 belongs to the Rab subfamily in the relevant extended familys of little gtp binding protein (being also referred to as the GTP enzyme) Ras.Rab GTP enzyme is that a class is regulated molecule, regulates the film transportation in eukaryotic cell, plays an important role in endocytosis and the transportation of biosynthetic albumen.New synthetic albumen all is subjected to the influence of little gtp binding protein Rab from the endoplasmic reticulum to the Golgi complex and the running of the vesicle that relates in the transportation of secretory vesicle.Rab shields when cell injury is repaired.Drought stress is induced the expression of little gtp binding protein Rab2, and it also is subjected to other external worlds to coerce the factor, as saline and alkaline (.Taylor ﹠amp such as ElaMizrahi-Aviv; Francis, 2002, that 13:295-300) waits inducing and expresses.Yet, up to now, also not relevant for the report of coding wheat little gtp binding protein Rab2 gene TaRab2.
Summary of the invention
At above-mentioned research background, inventor's further investigation, pass through library screening, obtain special EST (expressed sequence tag), utilize electronics extension and RT-PCR (Reverse Transcription-PCR inverse transcription polymerase chain reaction) technology successfully to clone the full length cDNA sequence of the little gtp binding protein Rab2 of wheat gene, called after TaRab2 (Triticum aestivum GTP-bindingprotein rab2), with the Rab2 GTP enzyme called after TaRab2 of its coding, thereby finished the present invention.
Should be pointed out that to comprise abiogenous polymorphism variation that for example the difference by the species that obtain proteinic biology, individuality etc. causes; The aminoacid sequence that obtains after aminoacid deletion, replacement and the insertion by the little gtp binding protein Rab2 of wheat that causes by induced mutationss such as site-directed mutagenesis method, random mutagenesis method processing introducing genetic mutation also belongs in the scope of protection of present invention.
Therefore, an object of the present invention is to provide a kind of nucleotide sequence, its coding has the following protein (i) of little gtp binding protein function or (ii):
(i) its aminoacid sequence is shown in SEQ ID NO:2;
(ii) one or more amino acid derived protein are replaced, lacked or insert to process in the aminoacid sequence that (i) limits.Preferred this nucleotides sequence is classified the little gtp binding protein Rab2 of the wheat gene TaRab2 with sequence shown in the SEQ ID NO:1 as.
A further object of the invention provides the application of the little gtp binding protein Rab2 of above-mentioned wheat gene TaRab2 in plant genetic engineering, it is characterized in that expressing in plant TaRab2.In a preferred embodiment, TaRab2 is used for the plant drought genetically engineered; In a further preferred embodiment, TaRab2 is used for adversity gene engineerings such as plant anti-salt and disease resistance.
The gene that directly pass through water stress method abduction delivering of TaRab2 gene in wheat, cloning first, because the little gtp binding protein Rab2 and the external world coerce the factor, be correlated with as arid, saline and alkaline, disease etc., and in the vegetable-protein transportation, play an important role.This gene of clone has important practical significance for utilizing transgenic method to cultivate Drought-resistance in Wheat, anti-salt or disease resistance new variety from wheat.
Description of drawings
The present invention is described in further detail below in conjunction with the drawings and specific embodiments, is that the present invention is limited but should not be construed as.
The full length cDNA sequence of Fig. 1 .TaRab2 gene and the aminoacid sequence of supposition thereof.This figure is the full length cDNA sequence of TaRab2 gene and the aminoacid sequence figure of supposition thereof.This full length gene 824bp, ORF district 633bp, 210 amino acid of encoding comprise the 5 ' UTR of 68bp and the 3 ' UTR of 124bp.The 68th of full length gene cDNA is that 5 ' of ORF holds first initiator codon ATG, and the 700th is 3 ' end terminator codon TAA, shown in runic among the figure.
Fig. 2: EST1 and electronics thereof extend the new nucleotide sequence EST2 in back.Wherein P1 is the 5 ' primer of RT-PCR, and P2 is its 3 ' primer, shown in runic among the figure.
Embodiment
To achieve these goals, a kind of preferred implementation of the present invention is as follows.
We are experiment material with wheat (Triticum aestivum) drought-resistant variety, by to the water stress 24h that made up and SSH (suppression subtractivehybridization) the cDNA library screening of 48h abduction delivering, obtained the EST of some important Drought-resistance in Wheat genes involveds; Utilize electronics to extend and the RT-PCR gene clone technology, in wheat, successfully cloned the full length cDNA sequence of little gtp binding protein Rab2 gene, called after TaRab2 (Triticumaestivum GTP-binding protein rab2).
We select No. 10 (Inst., of Breeds of Crops, Chinese Academy of Agriculture provides seed) with wheat (Triticum aestivum) drought-resistant variety drought is experiment material, make up SSH (suppression subtractive hybridization) the cDNA library of water stress 24h and 48h abduction delivering, by to library screening, obtained the EST of some important Drought-resistance in Wheat genes involveds, wherein, 48h SSHcDNA library made up [king's commentaries on classics etc. by this seminar in 2002, Acta Genetica Sinica, 2004,31 (8): 842-849], 24h SSH cDNA library made up (not delivering) by this seminar in 2003.Utilize electronics extend (this website, laboratory:
Http: // 192.168.0.150/) and RT-PCR gene clone technology, in wheat, successfully cloned the full length cDNA sequence of little gtp binding protein Rab2 gene, called after TaRab2 (Triticum aestivum GTP-binding protein rab2) (as accompanying drawing 1).M-MLV reverse transcription test kit, the Thermocycler PCR instrument of Biometra company and the archaeal dna polymerase of Promega company that Invitrogen company is adopted in reverse transcription increase Tm:55 ℃ 35 circulations.
Embodiment
We select No. 10 (Inst., of Breeds of Crops, Chinese Academy of Agriculture provides seed) with wheat (Triticum aestivum) drought-resistant variety drought is experiment material, { 48h SSH cDNA library is made up in 2002 by this seminar that [king changes etc. to SSH (suppression subtractive hybridization) cDNA library screening by water stress 24h that this seminar has been made up and 48h abduction delivering, Acta Genetica Sinica, 2004,31 (8): 842-849]; 24h SSH cDNA library made up (not delivering) by this seminar in 2003 }, adopt reverse northern (method reference: king's commentaries on classics etc., the initial analysis of wheat seedling water stress institute inducible gene expression spectrum, Acta Genetica Sinica, 2004,31 (8): 842-849) { hybridization is church damping fluid [1%BSA, 1mM EDTA (pH 8.0), 0.5M NaHPO with damping fluid with the Northern method
4Damping fluid (pH 7.2), 7%SDS], the label probe Prime-α-gene Labeling System of Promega company, isotropic substance α-
32The P-dCTP label probe } successfully screened this two cDNA expression libraries, obtained the EST of some important Drought-resistance in Wheat genes involveds; By being carried out Blast in GenBank, sequence relatively finds, wherein [(registration number: gi:722327), homology is 354/411 (86%) to the function of an EST (seeing accompanying drawing 1:EST1) with the little gtp binding protein Rab2B of corn gene Zm-Rab2-B with little gtp binding protein Rab2 gene-correlation; (registration number: gi:722325), homology is 324/374 (86%) with the little gtp binding protein Rab2A of corn gene Zm-Rab2-A; With the little gtp binding protein Rab2 gene of Sporobolus stapfianus (kind of plant in South Africa) (registration number: gi:4837726), homology 293/337 (86%)].So we are original template with this EST, utilize the method that electronics extends (this website, laboratory: http: // 192.168.0.150/) obtained a new est sequence (seeing accompanying drawing 2:EST2).Utilize primer select program in the DNAStar software at the two ends in this sequence encoding district design RT-PCR primer (see among the accompanying drawing 2:EST2 shown in the runic), (the M-MLV reverse transcription test kit of Invitrogen company is adopted in reverse transcription to carry out RT-PCR, the archaeal dna polymerase of Biometra company's T hermocycler PCR instrument and Promega company, the PCR annealing temperature is 55 ℃, 35 circulations), obtained the PCR product of 824bp, according to " Molecular Cloning " [Sambrook J, (Eds.) such as Frisch E F, second edition, cold spring port press, 1989] " described conventional molecular biology method connects product through carrier; transform; the PCR and the enzyme of plasmid are cut evaluation; [cloning vector is the pGM-T Easy of a Promega company carrier to sequencing, adopts T
4Dna ligase, transformed into escherichia coli JM 109 bacterial strains are cut evaluation with EcoR I (Promega company) enzyme, sequenator is 3730 sequenators of ABI company], thereby having cloned little gtp binding protein Rab2 gene, called after TaRab2 is as accompanying drawing 1.Relatively find by in GenBank, carrying out Blastn, the little gtp binding protein Rab2B of TaRab2 gene and corn gene Zm-Rab2-B homology reaches 89% (572/638), reach 90% (515/567) with the little gtp binding protein Rab2 of Sporobolus stapfianus homology, reach 89% (511/570) with the little gtp binding protein Rab2 of paddy rice (ORRab-2) homology.This full length gene 824bp, ORF district 633bp, 210 amino acid of encoding, its albumen theoretical molecular is MW=22.961kD, iso-electric point PI=7.27.
Annotate:
Total length RT-PCR primer:
P1?5’CTCGTCGCTGCTCCTCTCCAAATCC?3’
P2?3’GAAACAATAAAGTGCGCGGATACCT?5’
Should be appreciated that those skilled in the art can make various changes or modifications the present invention after having read above-mentioned teachings of the present invention, but the equivalent form of value of changing or revising drops on equally in the application's claims institute restricted portion.
Sequence table
＜110〉Inst., of Breeds of Crops, Chinese Academy of Agriculture
＜120〉the little gtp binding protein Rab2 of wheat gene TaRab2 and application thereof
<130>I040684
<160>4
<170>PatentIn?version?3.1
<210>1
<211>633
<212>DNA
＜213〉wheat
<220>
<221>CDS
<222>(1)..(633)
<223>
<400>1
atg?tcg?tac?gcc?tac?ctc?ttc?aag?tac?atc?atc?atc?ggc?gac?aca?ggt 48
Met?Ser?Tyr?Ala?Tyr?Leu?Phe?Lys?Tyr?Ile?Ile?Ile?Gly?Asp?Thr?Gly
1 5 10 15
gtc?ggc?aag?tcg?tgc?ctg?ctg?ctg?cag?ttc?acc?gac?aag?cgc?ttc?cag 96
Val?Gly?Lys?Ser?Cys?Leu?Leu?Leu?Gln?Phe?Thr?Asp?Lys?Arg?Phe?Gln
20 25 30
ccc?gtc?cac?gac?ctc?acc?atc?ggc?gtc?gag?ttc?ggc?gcc?cgg?atg?atc 144
Pro?Val?His?Asp?Leu?Thr?Ile?Gly?Val?Glu?Phe?Gly?Ala?Arg?Met?Ile
35 40 45
acc?atc?gac?aac?aag?ccc?atc?aag?ctc?cag?att?tgg?gac?acg?gca?ggc 192
Thr?Ile?Asp?Asn?Lys?Pro?Ile?Lys?Leu?Gln?Ile?Trp?Asp?Thr?Ala?Gly
50 55 60
cag?gag?tca?ttc?aga?tcg?ata?act?aga?tca?tac?tac?agg?gga?gct?gct 240
Gln?Glu?Ser?Phe?Arg?Ser?Ile?Thr?Arg?Ser?Tyr?Tyr?Arg?Gly?Ala?Ala
65 70 75 80
ggt?gct?ctt?ttg?gtt?tat?gac?atc?acc?agg?agg?gaa?acc?ttt?aat?cat 288
Gly?Ala?Leu?Leu?Val?Tyr?Asp?Ile?Thr?Arg?Arg?Glu?Thr?Phe?Asn?His
85 90 95
ctt?gca?agc?tgg?ctg?gaa?gat?gca?agg?caa?cat?gca?aat?gcc?aac?atg 336
Leu?Ala?Ser?Trp?Leu?Glu?Asp?Ala?Arg?Gln?His?Ala?Asn?Ala?Asn?Met
100 105 110
aca?ata?atg?cta?gtt?gga?aac?aaa?tgt?gac?cta?tcc?cat?agg?cgt?gcc 384
Thr?Ile?Met?Leu?Val?Gly?Asn?Lys?Cys?Asp?Leu?Ser?His?Arg?Arg?Ala
115 120 125
gtg?agc?ttc?gag?gaa?ggc?gag?cag?ttt?gcc?aag?gag?aat?ggt?ctc?atc 432
Val?Ser?Phe?Glu?Glu?Gly?Glu?Gln?Phe?Ala?Lys?Glu?Asn?Gly?Leu?Ile
130 135 140
ttt?atg?gag?gca?tct?gca?aaa?act?gca?caa?aat?gtc?gag?gag?ggc?ttt 480
Phe?Met?Glu?Ala?Ser?Ala?Lys?Thr?Ala?Gln?Asn?Val?Glu?Glu?Gly?Phe
145 150 155 160
gtc?aag?act?gct?gga?gca?ata?tac?aag?aaa?att?cag?gat?ggt?gtc?ttt 528
Val?Lys?Thr?Ala?Gly?Ala?Ile?Tyr?Lys?Lys?Ile?Gln?Asp?Gly?Val?Phe
165 170 175
gat?gta?tct?aat?gag?tcc?tat?gga?atc?aaa?gtt?ggc?tat?gca?gtt?cct 576
Asp?Val?Ser?Asn?Glu?Ser?Tyr?Gly?Ile?Lys?Val?Gly?Tyr?Ala?Val?Pro
180 185 190
ggc?caa?gct?gga?ggt?gct?ggt?gcc?tcg?tct?tcc?caa?ggt?ggc?agc?tgc 624
Gly?Gln?Ala?Gly?Gly?Ala?Gly?Ala?Ser?Ser?Ser?Gln?Gly?Gly?Ser?Cys
195 200 205
tgc?ggc?taa 633
Cys?Gly
210
<210>2
<211>210
<212>PRT
＜213〉wheat
<400>2
Met?Ser?Tyr?Ala?Tyr?Leu?Phe?Lys?Tyr?Ile?Ile?Ile?Gly?Asp?Thr?Gly
1 5 10 15
Val?Gly?Lys?Ser?Cys?Leu?Leu?Leu?Gln?Phe?Thr?Asp?Lys?Arg?Phe?Gln
20 25 30
Pro?Val?His?Asp?Leu?Thr?Ile?Gly?Val?Glu?Phe?Gly?Ala?Arg?Met?Ile
35 40 45
Thr?Ile?Asp?Asn?Lys?Pro?Ile?Lys?Leu?Gln?Ile?Trp?Asp?Thr?Ala?Gly
50 55 60
Gln?Glu?Ser?Phe?Arg?Ser?Ile?Thr?Arg?Ser?Tyr?Tyr?Arg?Gly?Ala?Ala
65 70 75 80
Gly?Ala?Leu?Leu?Val?Tyr?Asp?Ile?Thr?Arg?Arg?Glu?Thr?Phe?Asn?His
85 90 95
Leu?Ala?Ser?Trp?Leu?Glu?Asp?Ala?Arg?Gln?His?Ala?Asn?Ala?Asn?Met
100 105 110
Thr?Ile?Met?Leu?Val?Gly?Asn?Lys?Cys?Asp?Leu?Ser?His?Arg?Arg?Ala
115 120 125
Val?Ser?Phe?Glu?Glu?Gly?Glu?Gln?Phe?Ala?Lys?Glu?Asn?Gly?Leu?Ile
130 135 140
Phe?Met?Glu?Ala?Ser?Ala?Lys?Thr?Ala?Gln?Asn?Val?Glu?Glu?Gly?Phe
145 150 155 160
Val?Lys?Thr?Ala?Gly?Ala?Ile?Tyr?Lys?Lys?Ile?Gln?Asp?Gly?Val?Phe
165 170 175
Asp?Val?Ser?Asn?Glu?Ser?Tyr?Gly?Ile?Lys?Val?Gly?Tyr?Ala?Val?Pro
180 185 190
Gly?Gln?Ala?Gly?Gly?Ala?Gly?Ala?Ser?Ser?Ser?Gln?Gly?Gly?Ser?Cys
195 200 205
Cys?Gly
210
<210>3
<211>434
<212>DNA
＜213〉wheat EST1
<400>3
actacagggg?agctgctggc?gctcttttgg?tttatgacat?caccaggagg?gaaaccttta 60
atcatcttgc?aagctggctg?gaagatgcaa?ggcaacatgc?aaatgctaac?atgacaataa 120
tgctagttgg?aaacaaatgc?gacctatctc?ataggcgtgc?cgtgagcttc?gaggaaggcg 180
agcagttcgc?caaggagaat?ggtctcatct?ttatggaggc?gtctgcaaag?actgcacaaa 240
atgtcgagga?gggctttgtc?aagactgctg?gagcaatata?taagaaaatt?caggatggtg 300
tcttcgatgt?atctaatgag?tcctatggaa?tcaaagtggc?tatgcagttc?ctggccaagc 360
tggaggtgct?ggtgcctcgt?cttcccaagg?tggcagctgc?tgcggctaat?ggagcaatcc 420
atgatcaatg?atgt 434
<210>4
<211>1259
<212>DNA
＜213〉wheat EST2
<220>
<221>misc_feature
<222>(1183)..(1183)
<223>n＝a，g，c，t
<220>
<221>misc_feature
<222>(1222)..(1222)
<223>n＝a，g，c，t
<220>
<221>misc_feature
<222>(1247)..(1247)
<223>n＝a，g，c，t
<400>4
tggcttgctc?ccaccgtcct?ggtcggccga?gggacagcga?cattccgatt?tcgaaatcaa 60
taacccgcac?gcgaccgctg?cctggctaaa?tcgccgaagc?gaaccgcccc?cagatcaaga 120
tcagtccacc?acgtagccca?cgtctccttc?ctcccgaagt?aaacagagtt?ggaaatcccc 180
tctctctctc?cctcgtcgct?gctcctctcc?aaatcccctg?aacccgcctc?tccccggagg 240
aaccgcggcg?gaggagagat?gtcgtacgcc?tacctcttca?agtacatcat?catcggcgac 300
acaggtgtcg?gcaagtcgtg?cctgctgctg?cagttcaccg?acaagcgctt?ccagcccgtc 360
catgacctca?ccatcggcgt?cgagttcggc?gcccggatga?tcaccatcga?caacaagccc 420
atcaagctcc?agatttggga?cacggcaggc?caagaatcat?tcagatcgat?aactagatca 480
tactacaggg?gagctgctgg?cgctcttttg?gtttatgaca?tcaccaggag?ggaaaccttt 540
aatcatcttg?caagctggct?ggaagatgca?aggcaacatg?caaatgctaa?catgacaata 600
atgctagttg?gaaacaaatg?ygacctatct?cataggcgtg?ccgtgagctt?cgaggaaggc 660
gagcagttcg?ccaaggagaa?tggtctcatc?tttatggagg?crtctgcaaa?aactgcacaa 720
aatgtcgagg?agggctttgt?caagactgct?ggagcaatat?ataagaaaat?tcaggatggt 780
gtcttcgatg?tatctaatga?gtcctatgga?atcaaagttg?gctatgcagt?tcctggccaa 840
gctggaggtg?ctggtgcctc?gtcttcccaa?ggtggcagct?gctgcggcta?atggagcaat 900
ccatgatcaa?tgatgtacaa?aatcgcatgg?catgtgttac?aatacctgtt?acattatttc 960
gagttgccga?gtgattgtaa?ctccagggtg?ctttgttatt?tcacgcgcct?atggattatg 1020
ttccagtaag?ctagtcaaaa?taggttcagt?tcatttcctt?atactgtata?caatcactgt 1080
aatccatcag?atgcttccag?tcgtgtcatg?taggattcat?gaattatatc?catttgcatg 1140
tgtggattaa?aataagaatg?ttatgattca?atgtgtatgt?ttnaatcacc?tccaaacatt 1200
ttgggccctc?caagtttaaa?antaattgga?cacatttttt?ggttaanaaa?aaaaaaaaa 1259
Claims (5)
1. nucleotide sequence, its coding have little gtp binding protein function, the protein of aminoacid sequence shown in SEQ ID NO:2.
2. according to the nucleotide sequence of claim 1, it is the wheat little gtp binding protein Rab2 gene TaRab2 of sequence shown in SEQ ID NO:1.
3. the application of the little gtp binding protein Rab2 of wheat as claimed in claim 2 gene TaRab2 in plant genetic engineering is characterized in that expressing TaRab2 in plant.
4. according to the application of claim 3, wherein the little gtp binding protein Rab2 of wheat gene TaRab2 is used for the plant drought genetically engineered.
5. according to the application of claim 3, wherein the little gtp binding protein Rab2 of wheat gene TaRab2 is used for the plant stress-resistance genetically engineered.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410009930XA CN100529077C (en) | 2004-12-02 | 2004-12-02 | Wheat small GTP binding protein Rab2 gene TaRab2 and its use |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB200410009930XA CN100529077C (en) | 2004-12-02 | 2004-12-02 | Wheat small GTP binding protein Rab2 gene TaRab2 and its use |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1632121A CN1632121A (en) | 2005-06-29 |
| CN100529077C true CN100529077C (en) | 2009-08-19 |
Family
ID=34845536
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB200410009930XA Expired - Fee Related CN100529077C (en) | 2004-12-02 | 2004-12-02 | Wheat small GTP binding protein Rab2 gene TaRab2 and its use |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100529077C (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831438B (en) * | 2010-05-13 | 2012-01-25 | 山东大学 | Wheat introgression line response to abiotic stress regulatory gene tazf13 and application thereof |
| CN112661831A (en) * | 2021-01-22 | 2021-04-16 | 佛山科学技术学院 | Recombinant protein antigen, antibody and preparation method thereof |
-
2004
- 2004-12-02 CN CNB200410009930XA patent/CN100529077C/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 作物抗旱研究的现状与思考. 景蕊莲.干旱地区农业研究，第17卷第2期. 1999 |
| 作物抗旱研究的现状与思考. 景蕊莲.干旱地区农业研究，第17卷第2期. 1999 * |
| 小麦幼苗期水分胁迫所诱导基因表达谱的初步分析. 王转等.遗传学报，第31卷第8期. 2004 |
| 小麦幼苗期水分胁迫所诱导基因表达谱的初步分析. 王转等.遗传学报，第31卷第8期. 2004 * |
| 小麦苗期水分胁迫诱导差异表达cDNA的研究. 郭宾会等.生物技术通报，第2期. 2003 |
| 小麦苗期水分胁迫诱导差异表达cDNA的研究. 郭宾会等.生物技术通报，第2期. 2003 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1632121A (en) | 2005-06-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1314944A (en) | Stress tolerance and delayed senesence in plants | |
| CN101743314A (en) | Transgenic plants with increased stress tolerance and yield | |
| CN111778265B (en) | Mutant gene, mutant, expression vector and application of zearalenone oxidase | |
| CN107964543B (en) | Rice herbicide resistance ALS mutein, nucleic acid and its application | |
| CN111620935B (en) | Application of ZmCEP1 gene in regulation and control of corn kernel development | |
| CN110157718B (en) | Nitrate nitrogen regulation gene ZmNRG2.7 from corn and application thereof | |
| CN101775398B (en) | Stress tolerance genetic engineering application of NAC protein gene DlNAC of chrysanthemum | |
| CN107326032A (en) | A kind of gene and application for being used for cadmium pollution soil repair and improving cadmium-resistant vegetable | |
| CN109306353A (en) | Sesame anti-drought gene SiSAM1 and its application | |
| CN106834315B (en) | It is a kind of than white thorn NbCIPK25 gene and its expression albumen and application of undercuting | |
| Jian et al. | Study on mutations in ALS for resistance to tribenuron-methyl in Galium aparine L. | |
| CN101809155A (en) | Transgenic plants with increased stress tolerance and yield | |
| Liu et al. | Functional analysis of CgWRKY57 from Cymbidium goeringii in ABA response | |
| CN102161985B (en) | Rice root length relevant protein as well as coding gene and application thereof | |
| CN110452911A (en) | Corn ATP binding cassette transporter body protein raq gene ZmABCE2 and application | |
| CN100529077C (en) | Wheat small GTP binding protein Rab2 gene TaRab2 and its use | |
| CN104278053B (en) | A kind of method for improving drought tolerance in plants ability | |
| CN112795580B (en) | Pitaya gene HuAAE3 and application thereof in regulation and control of high temperature stress resistance of plants | |
| CN104498514A (en) | Nitraria tangutorum CBL-interacting protein kinase 9 (NtCIPK9) gene, expressed protein thereof and application thereof | |
| CN111909251B (en) | Rice selenium-rich gene OsHSE2-1 and application thereof | |
| CN107988233A (en) | Application of the soybean GmCRY1b genes in regulation and control plant height and flowering time | |
| CN104561040B (en) | Genes For Plant Tolerance hot radical is because of HTT3 and its application | |
| CN106916213A (en) | A kind of albumin A sT and its encoding gene and the application in plant stress tolerance | |
| CN110564737A (en) | Transcription factor HvTTG1 derived from barley genotype SLB and application thereof | |
| CN100363494C (en) | Galactinol synthetase gene of Boga crassifolia Hemsl and its coded protein and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20090819 Termination date: 20100104 |