CN100521963C - Method for preparing cloverleaf and endive plant antibiotic feed additive - Google Patents
Method for preparing cloverleaf and endive plant antibiotic feed additive Download PDFInfo
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- CN100521963C CN100521963C CNB2006100291394A CN200610029139A CN100521963C CN 100521963 C CN100521963 C CN 100521963C CN B2006100291394 A CNB2006100291394 A CN B2006100291394A CN 200610029139 A CN200610029139 A CN 200610029139A CN 100521963 C CN100521963 C CN 100521963C
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Abstract
The present invention relates to biotechnology, and is especially the preparation process of alfalfa-endive plant antibiotic feed additive. The alfalfa-endive plant antibiotic feed additive comprising alfalfa concentrate in 50-70 wt% and endive concentrate in 30-50 wt% has the preparation including the following steps: preparing alfalfa concentrate; preparing endive concentrate; and mixing the alfalfa concentrate and the endive concentrate in the said ratio to form the alfalfa-endive plant antibiotic feed additive. The alfalfa-endive plant antibiotic feed additive has powerful functions and can raise the immunity of farm animals.
Description
Technical field
That the present invention relates to is a kind of preparation method of biological technical field, specifically is a kind of preparation method of cloverleaf and endive plant anti-biotic feed additive.
Background technology
Alfalfa (Medicago sativa L. is hereinafter to be referred as clover) is perennial legume, and nutritive value is abundant, is the livestock and poultry forage grass of high-quality, and the good reputation of " king of herbage " is arranged.Alfalfa has nutrition, health care and physiology and regulates three big functions, aspect increase livestock and poultry body weight, enhancing immunity of livestock, the reduction disease incidence special effect is being arranged, and relevant research report is very many.
Lettuce dish [referring to sonchus oleraceus (Sonchus oleraceus L) or sowthistle (Patrinia scabbiosaefoliaFisch) or denticulate ixeris herb (Ixeris sonchifolia Hance)] is 1～2 year this plant of sward of composite family (Compositae), it is the animal and fowl fodder of high-quality, have antiviral, suppress gold-coloured staphylococci, Escherichia coli and streptococcus growth, promote hyperplasia, prevent effects such as degeneration of liver cells, illnesss such as enteritis, dysentery are had tangible prevention and result of treatment.
Find through literature search prior art, Zhao Kun etc. (2005) have reported with the clover polysaccharide at Agricultural University Of He'nan's journal the 39th volume 4 phases (426-429 page or leaf) and as the hardening agent of hog cholera lapinised virus vaccine the piglet of 60 age in days body weight, 18～20kg have been carried out immunity test research, the result shows, the clover polysaccharide can make the bone-marrow-derived lymphocyte of pig peripheral blood increase, the serum antibody level improves, and the hog cholera vaccine immune effect is had tangible invigoration effect.Lu Xinhua etc. (1999) have reported with the sonchus oleraceus water extract at traditional Chinese medical science research magazine the 12nd volume the 1st phase (11-12 page or leaf) and have fed mouse, can obviously increase the spleen weight and the thymic weight of children mouse in age, adult rats; Strengthen antifatigue, the anti-hungry ability of mouse; And the stress ability and the adaptive capacity that improve mouse.Alfalfa and sonchus oleraceus water extract all have obvious facilitation to improving animal immunizing power, and two kinds of plants exist than big-difference the action pathway of animal immunizing power and prevention from suffering from the diseases, alfalfa is to improve animal immunizing power by phagocytic index and the serum antibody level that improves T lymphocyte transformation rate, macrophage, sonchus oleraceus suppresses the generation that gold-coloured staphylococci, Escherichia coli and streptococcus growth improve immunity and the prevention intestines problem of animal then by enhancing antibody histocyte hyperplasia.Two kinds of plants are to improving animal immunizing power, and the function that reduces incidence of disease aspect has complementation.In further retrieval, yet there are no so far about alfalfa and lettuce dish water extract being hybridly prepared in proportion the report of the feed addictive that immunologic function is stronger, sphere of action is wider.
Summary of the invention
The present invention is directed to alfalfa and lettuce dish in the background technology (sonchus oleraceus, sowthistle and denticulate ixeris herb any) water extract to improving the complementarity of immunity of livestock, a kind of preparation method of cloverleaf and endive plant anti-biotic feed additive is provided, makes it have the effect that immunologic function is stronger, curative effect is wider.
The present invention is achieved by the following technical solutions, cloverleaf and endive plant anti-biotic feed additive of the present invention is made up of alfalfa concentrate and lettuce dish (sonchus oleraceus, sowthistle and denticulate ixeris herb any) concentrate, specifically form by the percentage by weight of 50-70%:30-50% by alfalfa concentrate and lettuce dish concentrate, this feed addictive of every 10ml contains alfalfa concentrate 5-7ml, lettuce dish concentrate 3-5ml, preparation process is as follows:
(1) the alfalfa concentrate is made: bright clover or the alfalfa hay powder that at first will pulverize mix with water, and mixed proportion is pressed alfalfa raw material g: water ml=1:10-20; Mixed clover raw material is through heat, boiling, cool off, filter, and is concentrated, cooling procedure again, and cooling fluid is the alfalfa concentrate;
(2) lettuce dish concentrate is made: bright lettuce dish or the lettuce dried vegetable grass meal that at first will pulverize mix with water, and ratio is pressed lettuce dish raw material g: water ml=1:10-20; Mixed clover raw material is through heat, boiling, cool off, filter, and is concentrated, cooling procedure again, and cooling fluid is lettuce dish concentrate;
(3) alfalfa concentrate and lettuce dish concentrate are mixed in proportion, form cloverleaf and endive plant anti-biotic feed additive.
Described step (1) is specially:
1. select fresh alfalfa raw material, be chopped into 3-5cm, perhaps the alfalfa hay powder is broken into alfalfa meal;
2. the ratio in bright clover (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed;
3. the bright clover that will pulverize mixes with water, makes bright grass and concentrates raw material, and the ratio of mixing is by light violet russian fenugreek herb raw material (g): water (ml)=1:10-20 (water yield that contains adding when pulverizing); Perhaps dried alfalfa meal is mixed with water, make hay and concentrate raw material, ratio is pressed alfalfa hay powder (g): water (ml)=1:10-20, concentrated raw material left standstill 1 hour;
4. the bright grass after will leaving standstill concentrates raw material or hay concentrates the raw material heating, boiled 1-3 hour, and cooling is filtered then;
5. filter liquor continues the 1/10-1/20 that heating is concentrated into the total water amount, is the alfalfa concentrate after the cooling, and the clover slag that filters out can be used as feed.
Described step (2) is specially:
1. selecting fresh lettuce dish (sonchus oleraceus, sowthistle and denticulate ixeris herb any) blade is raw material, is chopped into 3-5cm, perhaps lettuce dried vegetable grass meal is broken into lettuce dish grass meal;
2. the ratio in lettuce dish (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed;
3. add a certain amount of water in the lettuce dish of pulverizing, make bright grass and concentrate raw material, the ratio that adds entry is by lettuce dish raw material (g): water (ml)=1:10-20 (containing the water yield that adds when pulverizing); Perhaps dried lettuce dish grass meal is mixed with water, make hay and concentrate raw material, ratio is pressed lettuce dried vegetable grass meal (g): water (ml)=1:10-20, concentrated raw material left standstill 1 hour;
4. the bright grass after will leaving standstill concentrates raw material or hay concentrates the raw material heating, boiled 0.5-1.5 hour, and cooling is filtered then;
5. filter liquor continues the 1/10-1/20 that heating is concentrated into the total water amount, is lettuce dish concentrate after the cooling, and the lettuce dish slag that filters out can be used as feed.
Described step (3), be specially: alfalfa concentrate and lettuce dish concentrate are mixed in the ratio of 50-70%:30-50%, form cloverleaf and endive plant anti-biotic feed additive, wherein every this feed addictive of 10ml contains alfalfa concentrate 5-7ml, lettuce dish concentrate 3-5ml, polysaccharide, saponin and isoflavone content reach 10000～30000ppm, 2500～10000ppm and 4000～10000ppm respectively in the additive.The per kilogram feed adds the 10-30ml additive when feeding.
Characteristics of the present invention are: (1) raw materials for production are the edible herbage of livestock and poultry, to livestock and poultry and body harmless; (2) the antibiotic effect will be had in alfalfa and the lettuce dish, the extracts active ingredients that has complementary functions is come out, preparation becomes the tangible feed addictive of antibiotic function in proportion, and it is different from the method for alfalfa extract as additive for farm animal feed of using separately both at home and abroad.It is with better function that this feed addictive has antibiotic, more is of value to the effect that improves immunity of livestock.In 5 age in days chicken feeds, add this additive, the macrophage phagocytic index of chicken, active E-rosettes rate and ND antibody titer increase by 51.2～69.8%, 7.43～14.38% and 25.58～48.12% than control group respectively during 35 ages in days, and the weight ratio control group increases by 14.2～22.4%.
The specific embodiment
Provide following examples in conjunction with the inventive method and step:
Embodiment one,
1, the alfalfa concentrate is equipped with: (1) selects fresh alfalfa raw material, is chopped into 3-5cm, perhaps the alfalfa hay powder is broken into alfalfa meal; (2) ratio in bright clover (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed; (3) the bright clover that will pulverize mixes with water, makes bright grass and concentrates raw material, and the ratio of mixing is pressed light violet russian fenugreek herb raw material (g): water (ml)=1:10; Perhaps dried alfalfa meal is mixed with water, make hay and concentrate raw material, ratio is pressed alfalfa hay powder (g): water (ml)=1:10; (4) concentrated raw material of aquatic foods grass or hay are concentrated the raw material heating, boiled 1 hour, cooling is then filtered; (5) filter liquor continuation heating is concentrated into 1/10 of total water amount, is the alfalfa concentrate after the cooling.
2, lettuce dish concentrate is made: (1) selects fresh lettuce dish blade is raw material, is chopped into 3-5cm, perhaps lettuce dried vegetable grass meal is broken into lettuce dish grass meal; (2) ratio in lettuce dish (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed; (3) add a certain amount of water in the lettuce dish that will pulverize, make bright grass and concentrate raw material, the ratio that adds entry is by lettuce dish raw material (g): water (ml)=1:10; Perhaps dried lettuce dish grass meal is mixed with water, make hay and concentrate raw material, ratio is pressed lettuce dried vegetable grass meal (g): water (ml)=1:10; (4) concentrated raw material of aquatic foods grass or hay are concentrated the raw material heating, boiled 0.5 hour, cooling is then filtered; (5) filter liquor continuation heating is concentrated into 1/10 of total water amount, is lettuce dish concentrate after the cooling.
3, cloverleaf and endive plant anti-biotic feed additive is affected: the weight ratio of alfalfa concentrate and lettuce dish concentrate being pressed 50%:50% is formed, this feed addictive of every 10ml contains alfalfa concentrate 5ml, lettuce dish concentrate 5ml, polysaccharide, saponin and isoflavone content reach 23000ppm, 6200ppm and 6800ppm respectively in the additive.
Embodiment two
1, the alfalfa concentrate is made: (1) selects fresh alfalfa raw material, is chopped into 3-5cm, perhaps the alfalfa hay powder is broken into alfalfa meal; (2) ratio in bright clover (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed; (3) the bright clover that will pulverize mixes with water, makes bright grass and concentrates raw material, and the ratio of mixing is pressed light violet russian fenugreek herb raw material (g): water (ml)=1:15; Perhaps dried alfalfa meal is mixed with water, make hay and concentrate raw material, ratio is pressed alfalfa hay powder (g): water (ml)=1:15; (4) concentrated raw material of aquatic foods grass or hay are concentrated the raw material heating, boiled 2 hours, cooling is then filtered; (5) filter liquor continuation heating is concentrated into 1/10 of total water amount, is the alfalfa concentrate after the cooling.
2, lettuce dish concentrate is made: (1) selects fresh lettuce dish blade is raw material, is chopped into 3-5cm, perhaps lettuce dried vegetable grass meal is broken into lettuce dish grass meal; (2) ratio in lettuce dish (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed; (3) add a certain amount of water in the lettuce dish that will pulverize, make bright grass and concentrate raw material, the ratio that adds entry is by lettuce dish raw material (g): water (ml)=1:15; Perhaps dried lettuce dish grass meal is mixed with water, make hay and concentrate raw material, ratio is pressed lettuce dried vegetable grass meal (g): water (ml)=1:15; (4) concentrated raw material of aquatic foods grass or hay are concentrated the raw material heating, boiled 0.5 hour, cooling is then filtered; (5) filter liquor continuation heating is concentrated into 1/10 of total water amount, is lettuce dish concentrate after the cooling.
3, cloverleaf and endive plant anti-biotic feed additive is made: the weight ratio composition of alfalfa concentrate and lettuce dish concentrate press 65%:35%, this feed addictive of every 10ml contains alfalfa concentrate 6.5ml, lettuce dish concentrate 3.5ml, polysaccharide, saponin and isoflavone content reach 26000ppm, 8000ppm and 4130ppm respectively in the additive.
Embodiment three
1, the alfalfa concentrate is made: (1) selects fresh alfalfa raw material, is chopped into 3-5cm, perhaps the alfalfa hay powder is broken into alfalfa meal; (2) ratio in bright clover (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed; (3) the bright clover that will pulverize mixes with water, makes bright grass and concentrates raw material, and the ratio of mixing is pressed light violet russian fenugreek herb raw material (g): water (ml)=1:20; Perhaps dried alfalfa meal is mixed with water, make hay and concentrate raw material, ratio is pressed alfalfa hay powder (g): water (ml)=1:20; (4) concentrated raw material of aquatic foods grass or hay are concentrated the raw material heating, boiled 3 hours, cooling is then filtered; (5) filter liquor continuation heating is concentrated into 1/20 of total water amount, is the alfalfa concentrate after the cooling.
2, lettuce dish concentrate is made: (1) selects fresh lettuce dish blade is raw material, is chopped into 3-5cm, perhaps lettuce dried vegetable grass meal is broken into lettuce dish grass meal; (2) ratio in lettuce dish (g) and water (ml)=1:1 adds entry, fully mixes, with organizing pulverizer to smash to pieces at a high speed; (3) add a certain amount of water in the lettuce dish that will pulverize, make bright grass and concentrate raw material, the ratio that adds entry is by lettuce dish raw material (g): water (ml)=1:20; Perhaps dried lettuce dish grass meal is mixed with water, make hay and concentrate raw material, ratio is pressed lettuce dried vegetable grass meal (g): water (ml)=1:20; (4) concentrated raw material of aquatic foods grass or hay are concentrated the raw material heating, boiled 1.5 hours, cooling is then filtered; (5) filter liquor continuation heating is concentrated into 1/20 of total water amount, is lettuce dish concentrate after the cooling.
3, cloverleaf and endive plant anti-biotic feed additive is made: the weight ratio composition of alfalfa concentrate and lettuce dish concentrate press 40%:60%, this feed addictive of every 10ml contains alfalfa concentrate 4ml, lettuce dish concentrate 6ml, polysaccharide, saponin and isoflavone content reach 19000ppm, 4320ppm and 7260ppm respectively in the additive.
Claims (3)
1, a kind of preparation method of cloverleaf and endive plant anti-biotic feed additive, it is characterized in that, described cloverleaf and endive plant anti-biotic feed additive is made up of by the percentage by weight of 50-70%:30-50% alfalfa concentrate and lettuce dish concentrate, and preparation process is as follows:
(1) the alfalfa concentrate is made: bright clover or the alfalfa hay powder that at first will pulverize mix with water, and mixed proportion is pressed alfalfa raw material g: water ml=1:10-20; Mixed clover raw material is through heat, boiling, cool off, filter, and is concentrated, cooling procedure again, and cooling fluid is the alfalfa concentrate, and concrete steps are:
1. select fresh alfalfa raw material, be chopped into 3-5cm, perhaps the alfalfa hay powder is broken into alfalfa meal;
2. the ratio in bright clover g and water ml=1:1 adds entry, fully mixes, and smashs to pieces with pulverizer;
3. the bright clover that will pulverize mixes with water, makes bright grass and concentrates raw material, and the ratio of mixing is pressed light violet russian fenugreek herb raw material g: water ml=1:10-20 contains the water yield that adds when pulverizing; Maybe dried alfalfa meal is mixed with water, make hay and concentrate raw material, ratio is pressed alfalfa hay powder g: water ml=1:10-20 will concentrate raw material and leave standstill 1 hour;
4. the bright grass after will leaving standstill concentrates raw material or hay concentrates the raw material heating, boiled 1-3 hour, and cooling is filtered then;
5. filter liquor continues the 1/10-1/20 that heating is concentrated into the total water amount, and the cooling back is the alfalfa concentrate;
(2) lettuce dish concentrate is made: bright lettuce dish or the lettuce dried vegetable grass meal that at first will pulverize mix with water, and ratio is pressed lettuce dish raw material g: water ml=1:10-20; Mixed clover raw material is through heat, boiling, cool off, filter, and is concentrated, cooling procedure again, and cooling fluid is lettuce dish concentrate, and concrete steps are:
1. selecting fresh lettuce dish blade is raw material, is chopped into 3-5cm, perhaps lettuce dried vegetable grass meal is broken into lettuce dish grass meal;
2. the ratio in lettuce dish g and water ml=1:1 adds entry, fully mixes, and smashs to pieces with pulverizer;
3. add entry in the lettuce dish that will pulverize, and make bright grass and concentrate raw material, the ratio that adds entry is by lettuce dish raw material g: water ml=1:10-20 contains the water yield that adds when pulverizing; Maybe dried lettuce dish grass meal is mixed with water, make hay and concentrate raw material, ratio is pressed lettuce dried vegetable grass meal g: water ml=1:10-20, concentrated raw material left standstill 1 hour;
4. the bright grass after will leaving standstill concentrates raw material or hay concentrates the raw material heating, boiled 0.5-1.5 hour, and cooling is filtered then;
5. filter liquor continues the 1/10-1/20 that heating is concentrated into the total water amount, and the cooling back is a lettuce dish concentrate;
(3) alfalfa concentrate and lettuce dish concentrate are mixed in proportion, form cloverleaf and endive plant anti-biotic feed additive, polysaccharide, saponin and isoflavone content reach 10000～30000ppm, 2500～10000ppm and 4000～10000ppm respectively in the cloverleaf and endive plant anti-biotic feed additive that makes, the every 10ml of the cloverleaf and endive plant anti-biotic feed additive that makes contains alfalfa concentrate 5-7ml, lettuce dish concentrate 3-5ml.
2, the preparation method of cloverleaf and endive plant anti-biotic feed additive according to claim 1 is characterized in that, described lettuce dish is any in sonchus oleraceus, sowthistle and the denticulate ixeris herb.
3, the preparation method of cloverleaf and endive plant anti-biotic feed additive according to claim 1 is characterized in that, the per kilogram feed adds the 10-30ml cloverleaf and endive plant anti-biotic feed additive when feeding.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100291394A CN100521963C (en) | 2006-07-20 | 2006-07-20 | Method for preparing cloverleaf and endive plant antibiotic feed additive |
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|---|---|---|---|
| CNB2006100291394A CN100521963C (en) | 2006-07-20 | 2006-07-20 | Method for preparing cloverleaf and endive plant antibiotic feed additive |
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| CN1899086A CN1899086A (en) | 2007-01-24 |
| CN100521963C true CN100521963C (en) | 2009-08-05 |
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| CNB2006100291394A Expired - Fee Related CN100521963C (en) | 2006-07-20 | 2006-07-20 | Method for preparing cloverleaf and endive plant antibiotic feed additive |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101258896B (en) * | 2008-04-10 | 2010-11-17 | 上海交通大学 | Plant feed additive for reducing chicken total cholesterol level |
| CN101258897B (en) * | 2008-04-10 | 2010-09-29 | 上海交通大学 | Method for preparing plant feed additive for reducing chicken total cholesterol level |
| CN103385359B (en) * | 2013-08-07 | 2014-09-24 | 上海源耀生物股份有限公司 | Feeding attractant for piglets and production method thereof |
| CN111642623A (en) * | 2020-06-15 | 2020-09-11 | 沈阳化工大学 | Preparation method of plant compound feed additive |
| CN115316509A (en) * | 2022-07-21 | 2022-11-11 | 广西壮族自治区畜牧研究所 | Feed containing alfalfa extract and preparation method thereof |
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