CN100500835C - Preservative fluid for exfoliative cell - Google Patents
Preservative fluid for exfoliative cell Download PDFInfo
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- CN100500835C CN100500835C CNB2006100175919A CN200610017591A CN100500835C CN 100500835 C CN100500835 C CN 100500835C CN B2006100175919 A CNB2006100175919 A CN B2006100175919A CN 200610017591 A CN200610017591 A CN 200610017591A CN 100500835 C CN100500835 C CN 100500835C
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- preservative fluid
- edta
- ethylene diamine
- cell preservative
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Abstract
This invention discloses a preservation solution of exfoliated cells, which is composed of (by volume parts): methanol or ethanol 40-60, phosphate buffer solution or trihydroxymethyl aminomethane/HCl buffer solution 20-40, formaldehyde or acetone 5-10, and ethylenediamine tetraacetic acid dipotassium or Ethylenediamine tetraacetic acid disodium 5-10. This invention can reduce the cost of TCT consumable material by using inexpensive raw materials, and can reduce the consumption of consumable materials and time used on retreatment of the samples. This invention can keep the fixation of cells in reset conditions.
Description
Technical field
The present invention relates to a kind of exfoliated cell preservative fluid.
Background technology
Preservation liquid (the PreserveCyt of the present new Bai Shi of the U.S. (Tinpreper TCT) manufacturer production
TMSlution) be and the supporting patent reagent of its machine Tinpreper-2000, shortcoming: 1. cost an arm and a leg.2. flocks occurs after placing for some time for courageous and upright sample, operate the computer easy obstruction filtering net, cause film-making epithelial cell amount to reduce, therefore need to handle sample with Glacial acetic acid again and can go up machine.3. for the iuntercellular nucleus of piling up phenomenon appears increasing.
Summary of the invention
Purpose of the present invention just is that preserving the existing problem of liquid with regard to above-mentioned TCT improves: make it and can 1. reduce cost greatly.2. reduce flocculent precipitate, equal amount of bleeding and TCT preserve the liquid phase ratio.3. strengthen cell fixation, reduce nucleus and increase phenomenon.Thereby provide a kind of exfoliated cell preservative fluid of with low cost, better effects if.
Purpose of the present invention can realize by following measure:
The present invention contains following component, by volume part meter: 5 parts～10 parts of 40 parts～60 parts of methyl alcohol or ethanol, phosphate buffered saline buffer or 20 parts～40 parts of Tutofusin tris hydrochloride buffers, formaldehyde or 5 parts～10 parts in acetone, EDTA-2K or disodium ethylene diamine tetraacetate.
EDTA-2K or disodium ethylene diamine tetraacetate concentration are 5%～40% among the present invention.EDTA-2K or disodium ethylene diamine tetraacetate preferred concentration are 10%.Certainly, also can adopt other suitable concentration.One-tenth is grouped into and also includes hemolytic agent, and hemolytic agent is cyaniding high ferro or bromohexadecane base Trimethylamine 99, also can be other suitable hemolytic agents.
The present invention makes it to have following advantage owing to adopt above-mentioned prescription:
1, at first can reduce the price of TCT consumptive material, because above-mentioned starting material are common biochemical reagents, cost is low. and secondly can reduce and go out the dissatisfied rate of blood specimen for the first time, reduce the consumption and the waste of time of the consumptive material handle sample again and brought. because penetration power forces cell fixation abundant, keep cell fixing under virgin state as far as possible.
| Import Tinpreper TCT protection liquid protection liquid of the present invention |
| Hemoglobin 0.19 0.27 how many sample disposal rates of absorbance cell aggregation amount 11.8% 4.8% behind the courageous and upright sample floccule of 10 yuan/person-portion of 60 yuan/person-portion of price how many 30 μ l haemolysis |
Hemolytic agent: HiCN (cyaniding high ferro/bromohexadecane base Trimethylamine 99
2,1. reduce cost greatly.2. reduce flocculent precipitate, equal amount of bleeding and TCT preserve the liquid phase ratio.3. strengthen cell fixation, reduce nucleus and increase phenomenon.
Embodiment
The present invention does with detailed description below in conjunction with drawings and Examples:
Embodiment 1, the present invention are grouped into by following one-tenth substantially, by volume part is counted: methyl alcohol or ethanol (concentration 95-100%, as follows) 5 parts of 40 parts, phosphate buffered saline buffer or 20 parts of Tutofusin tris hydrochloride buffers (Tris/HCl damping fluid, as follows), formaldehyde or 5 parts in acetone, EDTA-2K or disodium ethylene diamine tetraacetate.More than each constituent concentration can adopt conventional concentration (as follows).
EDTA-2K or disodium ethylene diamine tetraacetate concentration are 5%～40% among the present invention, and preferred concentration is 10%.One-tenth is grouped into and also includes hemolytic agent, and hemolytic agent is cyaniding high ferro or bromohexadecane base Trimethylamine 99, also can be other suitable hemolytic agents.Hemolytic agent by volume part is 5 parts.
Embodiment 2,
The present invention is grouped into by following one-tenth substantially, meter by volume part: 7 parts of 25 parts of 45 parts of methyl alcohol or ethanol, phosphate buffered saline buffer or Tutofusin tris hydrochloride buffers (Tris/HCl damping fluid, as follows), formaldehyde or 7 parts in acetone, EDTA-2K or disodium ethylene diamine tetraacetate.
EDTA-2K or disodium ethylene diamine tetraacetate concentration are 5%～40% among the present invention, and preferred concentration is 10%.One-tenth is grouped into and also includes hemolytic agent, and hemolytic agent is cyaniding high ferro or bromohexadecane base Trimethylamine 99, also can be other suitable hemolytic agents.Hemolytic agent by volume part is 8 parts.
Embodiment 3,
The present invention is grouped into by following one-tenth substantially, meter by volume part: 8 parts of 30 parts of 50 parts of methyl alcohol or ethanol, phosphate buffered saline buffer or Tutofusin tris hydrochloride buffers (Tris/HCl damping fluid, as follows), formaldehyde or 8 parts in acetone, EDTA-2K or disodium ethylene diamine tetraacetate.
EDTA-2K or disodium ethylene diamine tetraacetate concentration are 5%～40% among the present invention, and preferred concentration is 10%.One-tenth is grouped into and also includes hemolytic agent, and hemolytic agent is cyaniding high ferro or bromohexadecane base Trimethylamine 99, also can be other suitable hemolytic agents.Hemolytic agent by volume part is 15 parts.
Embodiment 4,
The present invention is grouped into by following one-tenth substantially, meter by volume part: 9 parts of 35 parts of 55 parts of methyl alcohol or ethanol, phosphate buffered saline buffer or Tutofusin tris hydrochloride buffers (Tris/HCl damping fluid, as follows), formaldehyde or 9 parts in acetone, EDTA-2K or disodium ethylene diamine tetraacetate.
EDTA-2K or disodium ethylene diamine tetraacetate concentration are 5%～40% among the present invention, and preferred concentration is 10%.
Embodiment 5,
The present invention is grouped into by following one-tenth substantially, meter by volume part: 10 parts of 40 parts of 60 parts of methyl alcohol or ethanol, phosphate buffered saline buffer or Tutofusin tris hydrochloride buffers (Tris/HCl damping fluid, as follows), formaldehyde or 10 parts in acetone, EDTA-2K or disodium ethylene diamine tetraacetate.
EDTA-2K or disodium ethylene diamine tetraacetate concentration are 5%～40% among the present invention, and preferred concentration is 10%.
In the mentioned component parameter value of each component can be as required in its value range value arbitrarily.
Claims (6)
1, a kind of exfoliated cell preservative fluid, it is characterized in that: it comprises following composition, by volume part meter: 5 parts～10 parts of 40 parts～60 parts of methyl alcohol or ethanol, phosphate buffered saline buffer or 20 parts～40 parts of Tutofusin tris hydrochloride buffers, formaldehyde or 5 parts～10 parts in acetone, EDTA-2K or disodium ethylene diamine tetraacetate.
2, exfoliated cell preservative fluid according to claim 1 is characterized in that: described EDTA-2K or disodium ethylene diamine tetraacetate concentration are 5%～40%.
3, exfoliated cell preservative fluid according to claim 1 and 2 is characterized in that: described EDTA-2K or disodium ethylene diamine tetraacetate preferred concentration are 10%.
4, exfoliated cell preservative fluid according to claim 1 and 2 is characterized in that: described one-tenth is grouped into that also to include hemolytic agent part be 5 parts-15 parts by volume.
5, exfoliated cell preservative fluid according to claim 4 is characterized in that: described hemolytic agent is the cyaniding high ferro.
6, exfoliated cell preservative fluid according to claim 4 is characterized in that: described hemolytic agent is a bromohexadecane base Trimethylamine 99.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100175919A CN100500835C (en) | 2006-03-31 | 2006-03-31 | Preservative fluid for exfoliative cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100175919A CN100500835C (en) | 2006-03-31 | 2006-03-31 | Preservative fluid for exfoliative cell |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1834233A CN1834233A (en) | 2006-09-20 |
| CN100500835C true CN100500835C (en) | 2009-06-17 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100175919A Expired - Fee Related CN100500835C (en) | 2006-03-31 | 2006-03-31 | Preservative fluid for exfoliative cell |
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| CN (1) | CN100500835C (en) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101953334A (en) * | 2010-08-30 | 2011-01-26 | 南京卡博生物科技有限公司 | Cell preserving fluid for liquid-based cytologic diagnosis |
| CN101999343B (en) * | 2010-10-26 | 2013-11-06 | 深圳华大基因健康科技有限公司 | Cell preserving fluid and preparation method and use thereof |
| CN102318597B (en) * | 2011-08-19 | 2013-04-03 | 张雪云 | Liquid-based cell preservation liquid composite and preparation method thereof |
| CN103120153A (en) * | 2012-11-26 | 2013-05-29 | 刘召宏 | Exfoliative cells preserving fluid |
| CN104849122A (en) * | 2015-06-02 | 2015-08-19 | 蒋春亮 | Long-acting tumor tissue exudate sample preservation solution |
| CN105994252B (en) * | 2016-06-22 | 2019-07-02 | 杭州海世嘉生物科技有限公司 | A kind of Thinprep pap test saves liquid and preparation method thereof |
| CN110250160A (en) * | 2019-05-31 | 2019-09-20 | 广西壮族自治区人民医院 | Liquid based cell preservative fluid |
| CN111670896A (en) * | 2020-04-28 | 2020-09-18 | 浙江大学医学院附属妇产科医院 | Neutral buffer tissue fixing solution |
| CN114208812A (en) * | 2021-12-22 | 2022-03-22 | 桂林优利特医疗电子有限公司 | Liquid-based cell preservation solution |
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2006
- 2006-03-31 CN CNB2006100175919A patent/CN100500835C/en not_active Expired - Fee Related
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| Publication number | Publication date |
|---|---|
| CN1834233A (en) | 2006-09-20 |
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Granted publication date: 20090617 Termination date: 20110331 |