CN114208812A - Liquid-based cell preservation solution - Google Patents
Liquid-based cell preservation solution Download PDFInfo
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- CN114208812A CN114208812A CN202111576610.2A CN202111576610A CN114208812A CN 114208812 A CN114208812 A CN 114208812A CN 202111576610 A CN202111576610 A CN 202111576610A CN 114208812 A CN114208812 A CN 114208812A
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- cell preservation
- cell
- based cell
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- 239000007788 liquid Substances 0.000 title claims abstract description 64
- 239000003761 preservation solution Substances 0.000 title claims abstract description 63
- 239000000243 solution Substances 0.000 claims abstract description 59
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims abstract description 54
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 26
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims abstract description 23
- 229960001484 edetic acid Drugs 0.000 claims abstract description 23
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims abstract description 21
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 16
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims abstract description 16
- 229960004308 acetylcysteine Drugs 0.000 claims abstract description 16
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 claims abstract description 16
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 13
- 239000008103 glucose Substances 0.000 claims abstract description 13
- 229920000669 heparin Polymers 0.000 claims abstract description 13
- ZFGMDIBRIDKWMY-PASTXAENSA-N heparin Chemical compound CC(O)=N[C@@H]1[C@@H](O)[C@H](O)[C@@H](COS(O)(=O)=O)O[C@@H]1O[C@@H]1[C@@H](C(O)=O)O[C@@H](O[C@H]2[C@@H]([C@@H](OS(O)(=O)=O)[C@@H](O[C@@H]3[C@@H](OC(O)[C@H](OS(O)(=O)=O)[C@H]3O)C(O)=O)O[C@@H]2O)CS(O)(=O)=O)[C@H](O)[C@H]1O ZFGMDIBRIDKWMY-PASTXAENSA-N 0.000 claims abstract description 13
- 229960001008 heparin sodium Drugs 0.000 claims abstract description 13
- 239000011780 sodium chloride Substances 0.000 claims abstract description 13
- WSFSSNUMVMOOMR-NJFSPNSNSA-N methanone Chemical compound O=[14CH2] WSFSSNUMVMOOMR-NJFSPNSNSA-N 0.000 claims abstract description 12
- IXCSERBJSXMMFS-UHFFFAOYSA-N hydrogen chloride Substances Cl.Cl IXCSERBJSXMMFS-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229910000041 hydrogen chloride Inorganic materials 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000007853 buffer solution Substances 0.000 claims description 14
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- 239000008367 deionised water Substances 0.000 claims description 9
- 229910021641 deionized water Inorganic materials 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000002156 mixing Methods 0.000 claims description 7
- 229960000583 acetic acid Drugs 0.000 claims description 6
- 239000012362 glacial acetic acid Substances 0.000 claims description 6
- 239000008213 purified water Substances 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 claims 2
- 239000007864 aqueous solution Substances 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 claims 1
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 abstract description 33
- 239000003755 preservative agent Substances 0.000 abstract description 10
- 230000002335 preservative effect Effects 0.000 abstract description 10
- 210000003097 mucus Anatomy 0.000 abstract description 8
- 238000005054 agglomeration Methods 0.000 abstract description 5
- 230000002776 aggregation Effects 0.000 abstract description 5
- 230000009286 beneficial effect Effects 0.000 abstract description 5
- 239000006185 dispersion Substances 0.000 abstract description 5
- 102000004169 proteins and genes Human genes 0.000 abstract description 5
- 108090000623 proteins and genes Proteins 0.000 abstract description 5
- 230000001575 pathological effect Effects 0.000 abstract description 4
- 239000003146 anticoagulant agent Substances 0.000 abstract description 3
- 229940127219 anticoagulant drug Drugs 0.000 abstract description 3
- 239000003172 expectorant agent Substances 0.000 abstract description 3
- 229940066491 mucolytics Drugs 0.000 abstract description 3
- 238000001556 precipitation Methods 0.000 abstract description 3
- 239000000872 buffer Substances 0.000 abstract 1
- 230000006727 cell loss Effects 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 101
- 230000000694 effects Effects 0.000 description 8
- 238000010186 staining Methods 0.000 description 8
- 239000007983 Tris buffer Substances 0.000 description 7
- 206010054949 Metaplasia Diseases 0.000 description 5
- 230000015689 metaplastic ossification Effects 0.000 description 5
- 210000002919 epithelial cell Anatomy 0.000 description 3
- 210000004085 squamous epithelial cell Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 2
- 210000004369 blood Anatomy 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 210000003756 cervix mucus Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- FYFFGSSZFBZTAH-UHFFFAOYSA-N methylaminomethanetriol Chemical compound CNC(O)(O)O FYFFGSSZFBZTAH-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N1/00—Preservation of bodies of humans or animals, or parts thereof
- A01N1/02—Preservation of living parts
- A01N1/0205—Chemical aspects
- A01N1/021—Preservation or perfusion media, liquids, solids or gases used in the preservation of cells, tissue, organs or bodily fluids
- A01N1/0226—Physiologically active agents, i.e. substances affecting physiological processes of cells and tissue to be preserved, e.g. anti-oxidants or nutrients
Abstract
The invention relates to the technical field of pathological examination, in particular to a liquid-based cell preservation solution which comprises, by mass, 0.07-0.09% of sodium chloride, 1-5% of formaldehyde, 1-5% of glucose, 1-4% of ethylene diamine tetraacetic acid, 30-40% of methanol, 0.1-0.5% of dithiothreitol, 0.1-0.3% of acetylcysteine, 2-10% of heparin sodium, 0.8-12% of tris (hydroxymethyl) aminomethane and 0.8-12% of hydrogen chloride, adopts tris (hydroxymethyl) aminomethane and hydrogen chloride as acid-base buffer, adopts ethylene diamine tetraacetic acid as anticoagulant, dithiothreitol and acetylcysteine as mucolytic agents, and adopts formaldehyde as preservative, thereby not only maintaining the stability of cell structure and reducing caking precipitation and cell rupture loss of cell mucus, the cell preservative solution can also enable cells to be easily dyed, improve the clarity of cell flaking, and solve the problems that the prior cell preservative solution adopts methanol and added formaldehyde as a fixing preservative, can cause cell and protein mucus agglomeration, causes cell loss, and is not beneficial to cell dispersion and observation.
Description
Technical Field
The invention relates to the technical field of pathological examination, in particular to a liquid-based cell preservation solution.
Background
The liquid-based cell preservation solution is used for cytopathology examination of cervical mucus.
The existing cell preservation solution has the defects that the time for fixing and maintaining the stable cell structure is too short, cells are easy to deteriorate in the time of more than three days at room temperature, and 50% methanol is adopted to be added with formaldehyde to serve as a fixing preservative, so that the cells are easy to agglomerate and cake, protein mucus is also easy to agglomerate and deposit, the cell is not favorable for dispersion and observation of the cells, epithelial cells are covered, the cell quantity after the cells are sliced is directly influenced, and the cell quantity is lost.
Disclosure of Invention
The invention aims to provide a liquid-based cell preservation solution, and aims to solve the problems that the conventional cell preservation solution adopts methanol and formaldehyde added as a fixing preservative, cell and protein mucus are agglomerated, the cell amount is lost, and the dispersion and observation of cells are not facilitated.
In order to achieve the aim, the invention provides a liquid-based cell preservation solution which comprises, by mass, 0.07-0.09% of sodium chloride, 1-5% of formaldehyde, 1-5% of glucose, 1-4% of ethylene diamine tetraacetic acid, 30-40% of methanol, 0.1-0.5% of dithiothreitol, 0.1-0.3% of acetylcysteine, 2-10% of heparin sodium, 0.8-12% of tris (hydroxymethyl) aminomethane and 0.8-12% of hydrogen chloride.
The preparation method of the liquid-based cell preservation liquid comprises the following steps:
dissolving the tris (hydroxymethyl) aminomethane and the hydrogen chloride in 100ml of deionized water, and stirring for 10min to obtain a buffer solution;
dissolving the sodium chloride, the formaldehyde, the glucose, the ethylene diamine tetraacetic acid, the methanol and the heparin sodium in the buffer solution, and stirring for 10min to obtain a first solution;
dissolving the dithiothreitol and the acetylcysteine in 10ml of purified water, and stirring for 5min to obtain a second solution;
adding the second solution into a separating funnel, dropwise adding the second solution into the first solution within 30min, and uniformly mixing the first solution and the second solution to obtain a third solution;
and adjusting the pH value of the third solution to 6.5 by using glacial acetic acid to obtain the liquid-based cell preservation solution.
Wherein the temperature of the preparation environment of the liquid-based cell preservation solution is 15-30 ℃, and the air humidity is less than 70%.
According to the liquid-based cell preservation solution, the formaldehyde is used as a fixing agent of the liquid-based cell preservation solution, so that liquid-based cells in a specimen can be fixed quickly and effectively; the trihydroxymethyl aminomethane and the hydrogen chloride are used as acid-base buffer solution of the liquid-based cell preservation solution, which is helpful for keeping the constant pH value of the solution and preventing the cell from being broken due to the change of pH in cell fixation; ethylene diamine tetraacetic acid is used as an anticoagulant of the liquid-based cell preservation solution, so that blood in a specimen is prevented from being coagulated into blocks when the liquid-based cell preservation solution fixes cells; dissolving mucin in a specimen by using the dithiothreitol and the acetylcysteine as mucolytic agents of the liquid-based cell preservation solution; the formaldehyde is used as a preservative of the liquid-based cell preservation solution to prevent the deterioration of the liquid-based cell preservation solution; the liquid-based cell preservation solution provided by the invention can maintain the stability of a cell structure, reduce the agglomeration and precipitation of cell mucus and the cell rupture loss, can enable cells to be easily dyed, improve the clarity of cell flaking, facilitate the smooth progress of pathological examination work, is low in cost, is beneficial to popularization and use, and solves the problems that the existing cell preservation solution adopts methanol and formaldehyde added as a fixing preservative, causes the agglomeration of cells and protein mucus, causes the loss of cell amount, and is not beneficial to the dispersion and observation of cells.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a flow chart of a method for preparing a liquid-based cell preservation solution according to the present invention.
FIG. 2 is a graph showing the staining effect of a conventional cell preservation solution.
FIG. 3 is a graph showing the staining effect of a liquid-based cell preservation solution of the present invention on spider-like cells.
FIG. 4 is a graph showing the staining effect of a liquid-based cell preservation solution provided by the present invention on metaplasia of immature squamous epithelial cells.
FIG. 5 is a graph showing the staining effect of the liquid-based cell preservation solution provided by the invention on normal squamous metaplasia cells.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are illustrative and intended to be illustrative of the invention and are not to be construed as limiting the invention.
Referring to fig. 1, the present invention provides a liquid-based cell preservation solution, which comprises, by mass, 0.07% -0.09% of sodium chloride, 1% -5% of formaldehyde, 1% -5% of glucose, 1% -4% of ethylenediamine tetraacetic acid, 30% -40% of methanol, 0.1% -0.5% of dithiothreitol, 0.1% -0.3% of acetylcysteine, 2% -10% of heparin sodium, 0.8% -12% of tris (hydroxymethyl) aminomethane, and 0.8% -12% of hydrogen chloride.
Specifically, the formaldehyde is used as a fixing agent of the liquid-based cell preservation solution, so that liquid-based cells in a specimen can be fixed quickly and effectively; the Tris and the hydrogen chloride (HCl) are used as acid-base buffer solution of the liquid-based cell preservation solution, which helps to keep the pH value of the solution constant, so that cells are not broken due to pH change in cell fixation; ethylene Diamine Tetraacetic Acid (EDTA) is used as an anticoagulant of the liquid-based cell preservation solution, so that blood in the specimen is prevented from being coagulated into blocks when the liquid-based cell preservation solution fixes cells; dissolving mucin in a specimen by using the dithiothreitol and the acetylcysteine as mucolytic agents of the liquid-based cell preservation solution; the formaldehyde is used as a preservative of the liquid-based cell preservation solution to prevent the deterioration of the liquid-based cell preservation solution; the liquid-based cell preservation solution provided by the invention can maintain the stability of a cell structure, reduce the agglomeration and precipitation of cell mucus and the cell rupture loss, can enable cells to be easily dyed, improve the clarity of cell flaking, facilitate the smooth progress of pathological examination work, is low in cost, is beneficial to popularization and use, and solves the problems that the existing cell preservation solution adopts methanol and formaldehyde added as a fixing preservative, causes the agglomeration of cells and protein mucus, causes the loss of cell amount, and is not beneficial to the dispersion and observation of cells.
Further, the preparation method of the liquid-based cell preservation solution comprises the following steps:
s101, dissolving the tris (hydroxymethyl) aminomethane and the hydrogen chloride in 100ml of deionized water, and stirring for 10min to obtain a buffer solution;
s102, dissolving the sodium chloride, the formaldehyde, the glucose, the ethylene diamine tetraacetic acid, the methanol and the heparin sodium in the buffer solution, and stirring for 10min to obtain a first solution;
s103, dissolving the dithiothreitol and the acetylcysteine in 10ml of purified water, and stirring for 5min to obtain a second solution;
s104, adding the second solution into a separating funnel, dropwise adding the second solution into the first solution within 30min, and uniformly mixing the first solution and the second solution to obtain a third solution;
and S105, adjusting the pH value of the third solution to 6.5 by using glacial acetic acid to obtain the liquid-based cell preservation solution.
Specifically, the temperature of the preparation environment of the liquid-based cell preservation solution is 15-30 ℃, and the air humidity is less than 70%.
The first embodiment is as follows:
the components of the liquid-based cell preservation solution in the embodiment comprise: the liquid base cell preservation solution comprises 0.09g of sodium chloride, 2.5g of formaldehyde, 2.5g of glucose, 1.5g of Ethylene Diamine Tetraacetic Acid (EDTA), 25ml of methanol, 0.3g of dithiothreitol, 0.1g of acetylcysteine, 3.5g of heparin sodium, 2.5g of Tris (hydroxymethyl) aminomethane (Tris) and 4.6g of hydrogen chloride (HCl), water is the deionized water, and the pH value of the liquid base cell preservation solution is 6.5.
The preparation method of the liquid-based cell preservation solution of the embodiment comprises the following steps:
a. dissolving the Tris (hydroxymethyl) aminomethane (Tris) and the hydrogen chloride (HCl) in the deionized water of 100ml according to the formula ratio, and stirring for 10min to obtain the buffer solution.
b. Dissolving the sodium chloride, the formaldehyde, the glucose, the Ethylene Diamine Tetraacetic Acid (EDTA), the methanol and the heparin sodium in the buffer solution according to the formula amount, and stirring for 15min to obtain a first solution;
c. stirring the dithiothreitol and the acetylcysteine with the formula amount in 10ml of the purified water for 5min to obtain a second solution;
d. adding the second solution into a separating funnel, dropwise adding the second solution into the first solution within 30min, and uniformly mixing the first solution and the second solution to obtain a third solution
e. And finally, adjusting the pH value of the third solution to 6.5 by using glacial acetic acid to obtain the liquid-based cell preservation solution.
The steps are all carried out at the temperature of 15-30 ℃ and the humidity of less than 70%.
Example two:
the components of the liquid-based cell preservation solution in the embodiment comprise: the liquid base cell preservation solution comprises 0.07g of sodium chloride, 2g of formaldehyde, 3g of glucose, 2g of Ethylene Diamine Tetraacetic Acid (EDTA), 30ml of methanol, 0.3g of dithiothreitol, 0.2g of acetylcysteine, 5g of heparin sodium, 1.5g of Tris (hydroxymethyl) aminomethane (Tris) and 2.8g of hydrogen chloride (HCl), water is the deionized water, and the pH value of the liquid base cell preservation solution is 6.5.
The preparation method of the liquid-based cell preservation solution of the embodiment comprises the following steps:
a. dissolving the Tris (hydroxymethyl) aminomethane (Tris) and the hydrogen chloride (HCl) in the deionized water in a formula amount of 100ml, and stirring for 10min to obtain a buffer solution
b. Dissolving the sodium chloride, the formaldehyde, the glucose, the Ethylene Diamine Tetraacetic Acid (EDTA), the methanol and the heparin sodium in a buffer solution according to the formula amount, and stirring for 18min to obtain a first solution;
c. stirring the dithiothreitol and the acetylcysteine with the formula amount in 10ml of purified water for 5min to obtain a second solution;
d. adding the second solution into a separating funnel, dropwise adding the second solution into the first solution within 30min, and uniformly mixing the first solution and the second solution to obtain a third solution
e. And finally, adjusting the pH value of the third solution to 6.5 by using glacial acetic acid to obtain the liquid-based cell preservation solution.
The steps are all carried out at the temperature of 15-30 ℃ and the humidity of less than 70%.
Example three:
the components of the liquid-based cell preservation solution in the embodiment comprise: the sodium chloride is 0.09g, the formaldehyde is 2.2g, the glucose is 4.3g, the Ethylene Diamine Tetraacetic Acid (EDTA) is 3.5g, the methanol is 35ml, the dithiothreitol is 0.38g, the acetylcysteine is 0.26g, the heparin sodium is 5.6g, the Tris (hydroxymethyl) aminomethane (Tris) is 1.8g, the hydrogen chloride (HCl) is 2.5g, the water is the deionized water, and the pH value of the liquid-based cell preservation solution is 6.5.
The preparation method of the liquid-based cell preservation solution of the embodiment comprises the following steps:
a. dissolving the Tris (hydroxymethyl) aminomethane (Tris) and the hydrogen chloride (HCl) in the deionized water of 100ml according to the formula ratio, and stirring for 10min to obtain a buffer solution
b. Dissolving the sodium chloride, the formaldehyde, the glucose, the Ethylene Diamine Tetraacetic Acid (EDTA), the methanol and the heparin sodium in a buffer solution according to the formula amount, and stirring for 15min to obtain a first solution;
c. stirring the dithiothreitol and the acetylcysteine with the formula amount in 10ml of purified water for 5min to obtain a second solution;
d. adding the second solution into a separating funnel, dropwise adding the second solution into the first solution within 30min, and uniformly mixing the first solution and the second solution to obtain a third solution
e. And finally, adjusting the pH value of the third solution to 6.5 by using glacial acetic acid to obtain the liquid-based cell preservation solution.
The steps are all carried out at the temperature of 15-30 ℃ and the humidity of less than 70%.
As shown in fig. 2, fig. 2 is a graph showing the staining effect of the conventional cell preservation solution, and in fig. 2, inflammatory cells cover 75% of epithelial cells and are interpreted as unsatisfactory samples;
FIG. 3 is a graph showing the staining effect of a liquid-based cell preservation solution on spider-like cells, wherein the spider-like cells are immature cells, and when a sample is taken, the cells are normal due to the fact that a scraper is too forcefully used, and the cells cannot be interpreted as abnormal epithelial cells;
FIG. 4 is a graph showing the staining effect of the liquid-based cell preservation solution on metaplasia of immature squamous epithelial cells, wherein metaplasia nuclei of the immature squamous epithelial cells are normal and belong to normal cells. Immature squamous metaplastic cells can also form prisms.
FIG. 5 is a graph showing the staining effect of the liquid-based cell preservation solution on normal squamous metaplasia cells.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (3)
1. The liquid-based cell preservation solution is characterized by comprising, by mass, 0.07% -0.09% of sodium chloride, 1% -5% of formaldehyde, 1% -5% of glucose, 1% -4% of ethylenediamine tetraacetic acid, 30% -40% of methanol, 0.1% -0.5% of dithiothreitol, 0.1-0.3% of acetylcysteine, 2% -10% of heparin sodium, 0.8% -12% of tris (hydroxymethyl) aminomethane, and 0.8% -12% of hydrogen chloride.
2. A method for producing a liquid-based cell preservation solution, which is used for producing the liquid-based cell preservation solution according to claim 1,
the preparation method of the liquid-based cell preservation liquid comprises the following steps:
dissolving the tris (hydroxymethyl) aminomethane and the hydrogen chloride in 100ml of deionized water, and stirring for 10min to obtain a buffer solution;
dissolving the sodium chloride, the formaldehyde, the glucose, the ethylene diamine tetraacetic acid, the methanol and the heparin sodium in the buffer solution, and stirring for 10min to obtain a first solution;
dissolving the dithiothreitol and the acetylcysteine in 10ml of purified water, and stirring for 5min to obtain a second solution;
adding the second solution into a separating funnel, dropwise adding the second solution into the first solution within 30min, and uniformly mixing the first solution and the second solution to obtain a third solution;
and adjusting the pH value of the third solution to 6.5 by using glacial acetic acid to obtain the liquid-based cell preservation solution.
3. The method for producing a liquid-based cell preservation solution according to claim 2, wherein the cell preservation solution is prepared by mixing the aqueous solution of the compound of formula (I),
the temperature of the preparation environment of the liquid-based cell preservation liquid is 15-30 ℃, and the air humidity is less than 70%.
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| CN202111576610.2A CN114208812A (en) | 2021-12-22 | 2021-12-22 | Liquid-based cell preservation solution |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834233A (en) * | 2006-03-31 | 2006-09-20 | 李肖甫 | Preservative fluid for exfoliative cell |
| CN102113481A (en) * | 2010-12-24 | 2011-07-06 | 厦门迈威生物科技有限公司 | Exfoliated cell preservation solution and preparation method thereof |
| US20130059330A1 (en) * | 2010-01-27 | 2013-03-07 | Novacyt | Fixing solution for biological cells |
| CN111972400A (en) * | 2020-08-18 | 2020-11-24 | 武汉瑞新昌生物科技有限公司 | Treatment and preservation reagent for liquid-based cells and microorganisms |
-
2021
- 2021-12-22 CN CN202111576610.2A patent/CN114208812A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834233A (en) * | 2006-03-31 | 2006-09-20 | 李肖甫 | Preservative fluid for exfoliative cell |
| US20130059330A1 (en) * | 2010-01-27 | 2013-03-07 | Novacyt | Fixing solution for biological cells |
| CN102113481A (en) * | 2010-12-24 | 2011-07-06 | 厦门迈威生物科技有限公司 | Exfoliated cell preservation solution and preparation method thereof |
| CN111972400A (en) * | 2020-08-18 | 2020-11-24 | 武汉瑞新昌生物科技有限公司 | Treatment and preservation reagent for liquid-based cells and microorganisms |
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