CN101953334A - Cell preserving fluid for liquid-based cytologic diagnosis - Google Patents
Cell preserving fluid for liquid-based cytologic diagnosis Download PDFInfo
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- CN101953334A CN101953334A CN201010265564XA CN201010265564A CN101953334A CN 101953334 A CN101953334 A CN 101953334A CN 201010265564X A CN201010265564X A CN 201010265564XA CN 201010265564 A CN201010265564 A CN 201010265564A CN 101953334 A CN101953334 A CN 101953334A
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Abstract
The invention relates to cell preserving fluid for liquid-based cytologic diagnosis. The cell preserving fluid comprises glucan, 4-hydroxyethyl piperazine ethanesulfonic acid, ethanol, formaldehyde, potassium dihydrogen phosphate and disodium ethylene diaminetetraacetate, wherein the glucan is at least one of glucan T4, glucan T6 and glucan T9. The problems that the cell shape is easy to change and the cell preserving fluid cannot be preserved for long time in the process of preparing sheets at present are solved.
Description
Technical field
The invention belongs to the cell-preservation liquid field, particularly relate to a kind of cell-preservation liquid of liquid based cytology diagnosis usefulness.
Background technology
The cell-preservation liquid of liquid based cytology diagnosis usefulness is meant the solution that is used to preserve from the cell of human body collection, detects with the liquid based cytology of carrying out cell, thereby carries out the cell pathology diagnosis.Liquid based cytology detects and is used to detect gynaecology's sample, for example cervical cell, and non-gynaecology sample, for example saliva, urine etc.In liquid based cytology detects, whether there is pathological cells by the cell that uses microscopic examination to collect.Detect the cell-preservation liquid of the cell that a kind of preservation of needs is collected in order to carry out above liquid based cytology.
The existing liquid of preserving easily makes cell form in the film-making process change, and loses diagnostic value.40～50% alcohols at present commonly used are as preserving liquid, though can fix mobile high protein, protein just forms sediments immediately, causes cytomorphosis.The content that uses alcohols is also arranged less than 20%, but cause cell not preserve for a long time, easily make cell degradation.
Summary of the invention
The purpose of this invention is to provide a kind of cell-preservation liquid of liquid based cytology diagnosis usefulness, overcome existing shortcoming, make it to keep cell form in the film-making process not change and preservation for a long time.
To achieve these goals, the technical scheme that the present invention proposes is: a kind of cell-preservation liquid of liquid based cytology diagnosis usefulness, it comprises: glucan, the 4-HEPES, ethanol, formaldehyde, potassium dihydrogen phosphate and disodium ethylene diamine tetraacetate, wherein the content range of glucan is 8～18g/L, the content range of 4-HEPES is 25～35g/L, the content range of ethanol is 100～180g/L, the content range of formaldehyde is 40～80g/L, the content range of potassium dihydrogen phosphate is 15～35g/L, the content range of disodium ethylene diamine tetraacetate is 5～10g/L.Described glucan is a kind of among glucan T4, glucan T6 or the glucan T9 at least.
Glucan is a drying retarder in the cell-preservation liquid, can make cell keep moistening in the film-making process, thereby form is not changed, and keeps diagnostic value.4-HEPES and potassium dihydrogen phosphate are used to control the pH value of preserving liquid.Ethanol and formaldehyde can make cell fixation, and anti-corrosion function is arranged simultaneously.Disodium ethylene diamine tetraacetate plays blood coagulation resisting function.These materials can keep cellular morphology not change in described content range and can preserve for a long time.The present invention has just overcome existing shortcoming like this, makes it to keep cell form in the film-making process not change and can preserve for a long time.
Embodiment
The basic composition of the cell-preservation liquid of a kind of liquid based cytology diagnosis usefulness of embodiment comprises: glucan, 4-HEPES, ethanol, formaldehyde, potassium dihydrogen phosphate and disodium ethylene diamine tetraacetate.The glucan of present embodiment is selected glucan T4, and wherein the content of glucan T4 is that the content of 12g/L, 4-HEPES is that the content of 30g/L, ethanol is that the content of 150g/L, formaldehyde is that the content of 50g/L, potassium dihydrogen phosphate is that the content of 20g/L, disodium ethylene diamine tetraacetate is 8g/L.
The cell-preservation liquid intensive amount is that the glucan of 12g/L is a drying retarder, can make cell keep moistening in the film-making process, thereby form is not changed, and keeps diagnostic value.Content be the 4-HEPES of 30g/L and potassium dihydrogen phosphate that content is 20g/L be used to control preserve liquid pH value about 7.Content is that the ethanol of 150g/L and formaldehyde that content is 50g/L can make cell fixation, and anti-corrosion function is arranged simultaneously.Content is that the disodium ethylene diamine tetraacetate of 8g/L plays blood coagulation resisting function.These materials can keep cellular morphology not change in described content range and can preserve for a long time.
Claims (2)
1. the cell-preservation liquid of liquid based cytology diagnosis usefulness, it is characterized in that it comprises: glucan, 4-HEPES, ethanol, formaldehyde, potassium dihydrogen phosphate and disodium ethylene diamine tetraacetate, wherein the content range of glucan is that the content range of 8～18g/L, 4-HEPES is that the content range of 25～35g/L, ethanol is that the content range of 100～180g/L, formaldehyde is that the content range of 40～80g/L, potassium dihydrogen phosphate is that the content range of 15～35g/L, disodium ethylene diamine tetraacetate is 5～10g/L.
2. the cell-preservation liquid of a kind of liquid based cytology diagnosis usefulness according to claim 1 is characterized in that described glucan is a kind of among glucan T4, glucan T6 or the glucan T9 at least.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010265564XA CN101953334A (en) | 2010-08-30 | 2010-08-30 | Cell preserving fluid for liquid-based cytologic diagnosis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201010265564XA CN101953334A (en) | 2010-08-30 | 2010-08-30 | Cell preserving fluid for liquid-based cytologic diagnosis |
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| Publication Number | Publication Date |
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| CN101953334A true CN101953334A (en) | 2011-01-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| CN201010265564XA Pending CN101953334A (en) | 2010-08-30 | 2010-08-30 | Cell preserving fluid for liquid-based cytologic diagnosis |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103674640A (en) * | 2013-11-28 | 2014-03-26 | 安徽信灵检验医学科技有限公司 | Formaldehyde molecule membranization buffer solution and preparation method thereof |
| CN103704200A (en) * | 2013-10-25 | 2014-04-09 | 无锡灵锡医疗器械科技有限公司 | Method for antibacterial stabilization of cell tissue sample compositions and structure |
| CN104585162A (en) * | 2014-12-30 | 2015-05-06 | 李东 | Novel environment-friendly nontoxic liquid-based cell preservation liquid |
| CN110286009A (en) * | 2019-06-06 | 2019-09-27 | 强安医疗器械有限公司 | A kind of mucous membrane of rectum cast-off cells sample pretreatment solution and preparation method thereof |
| CN113925049A (en) * | 2021-12-17 | 2022-01-14 | 广东乾晖生物科技有限公司 | Cell preservation solution for maintaining cell activity and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834233A (en) * | 2006-03-31 | 2006-09-20 | 李肖甫 | Preservative fluid for exfoliative cell |
| CN101363011A (en) * | 2008-09-17 | 2009-02-11 | 上海艾迪康临床检验中心有限公司 | Cervical exfoliated cell preservative fluid |
-
2010
- 2010-08-30 CN CN201010265564XA patent/CN101953334A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1834233A (en) * | 2006-03-31 | 2006-09-20 | 李肖甫 | Preservative fluid for exfoliative cell |
| CN101363011A (en) * | 2008-09-17 | 2009-02-11 | 上海艾迪康临床检验中心有限公司 | Cervical exfoliated cell preservative fluid |
Non-Patent Citations (2)
| Title |
|---|
| 徐智勇等: "角膜中期保存液添加bFGF的质量浓度分析", 《眼科研究》, vol. 25, no. 5, 31 May 2007 (2007-05-31), pages 329 * |
| 郑佳予等: "离体心脏保存液的现状及研究进展", 《中国临床医学》, vol. 11, no. 5, 31 October 2004 (2004-10-31), pages 682 - 683 * |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103704200A (en) * | 2013-10-25 | 2014-04-09 | 无锡灵锡医疗器械科技有限公司 | Method for antibacterial stabilization of cell tissue sample compositions and structure |
| CN103674640A (en) * | 2013-11-28 | 2014-03-26 | 安徽信灵检验医学科技有限公司 | Formaldehyde molecule membranization buffer solution and preparation method thereof |
| CN104585162A (en) * | 2014-12-30 | 2015-05-06 | 李东 | Novel environment-friendly nontoxic liquid-based cell preservation liquid |
| CN104585162B (en) * | 2014-12-30 | 2017-06-16 | 李东 | A kind of nontoxic liquid based cell preservative fluid of novel environment friendly |
| CN110286009A (en) * | 2019-06-06 | 2019-09-27 | 强安医疗器械有限公司 | A kind of mucous membrane of rectum cast-off cells sample pretreatment solution and preparation method thereof |
| CN113925049A (en) * | 2021-12-17 | 2022-01-14 | 广东乾晖生物科技有限公司 | Cell preservation solution for maintaining cell activity and preparation method and application thereof |
| CN113925049B (en) * | 2021-12-17 | 2022-04-29 | 广东乾晖生物科技有限公司 | Cell preservation solution for maintaining cell activity and preparation method and application thereof |
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Application publication date: 20110126 |