CN100497588C - Fungus ball food production method and air-lift culturing tank for producing same - Google Patents

Fungus ball food production method and air-lift culturing tank for producing same Download PDF

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CN100497588C
CN100497588C CNB2006101368035A CN200610136803A CN100497588C CN 100497588 C CN100497588 C CN 100497588C CN B2006101368035 A CNB2006101368035 A CN B2006101368035A CN 200610136803 A CN200610136803 A CN 200610136803A CN 100497588 C CN100497588 C CN 100497588C
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lift culturing
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杨德俊
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08—Air lift
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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Abstract

The invention discloses a manufacturing method of bacterial-ball food, which comprises the following steps: preparing culture medium; making liquid fungus; fermenting grade by grade continuously; sieving; grading; proceeding vacuum packing; making the diameter and quality of bacterial-ball satisfy edible need; fitting for industrial manufacturing. The invention also provides gas-lift culture tank of bacterial-ball food, which comprises the following parts: tank, inlet, outlet, grading sieving board, vent on the top of tank, conduct in the tank or besides the tank vertically, air distributor on the bottom of conduct to opposite the tank bottom.

Description

The air-lift culturing tank of bacterium ball food-stuff production method and production bacterium ball food
Technical field
The present invention relates to that industrialization is edible, the technology and the corresponding incubator of the production of hybrid seeds of medicinal fungi liquid, be specially a kind of bacterium ball food-stuff production method and produce the air-lift culturing tank of bacterium ball food.
Background technology
In prior art, the production of edible mushrooms has various ways, and the edible fungus liquid substratum is generally used Semen Maydis powder, starch, wheat bran etc., and because of its molecular weight is big, mycelium was difficult to utilize at the cultivation initial stage, thereby the incubation growth cycle is slow.In addition, the incubator of producing liquid spawn all adopts the mechanical stirring principle, and this makes the incubator volume big, and cost increases, and is easy to generate pollution.These problems all are unfavorable for the suitability for industrialized production of edible mushrooms.
Chinese patent 00123385.8 discloses a kind of method edible, the medicinal fungi liquid spawn of producing, this method mainly is to utilize alcohol waste lees solution adding starch, sucrose, yeast powder to be mixed with substratum, prepare the level liquid bacterial classification earlier, utilize this substratum then, adopt deep layer fermenting process, produce food, medicinal fungi bacterial classification, realize the batch production production of bacterial classification.Chinese patent 01114001.1 discloses a kind of edible fungi liquid strain production method, the substratum of this method is murphy juice, brown sugar, peptone, sal epsom, dipotassium hydrogen phosphate, zinc sulfate, VB1, agar etc., the incubator of this method is that the air riser tube is arranged in tank body, be air nozzle below the riser tube, Electric heating bar is arranged in the tank body.Cultivated through 3～5 days, produce qualified bacterial classification, this bacterial classification also will obtain product through solid state cultivation.Use the bacterium food of aforesaid method and device fabrication still not ideal enough.Mainly show not of uniform size as edible, medicinal bacterium sphere volume, quality neither the meat tissue.
Summary of the invention
Technical problem to be solved by this invention provides a kind of bacterium ball food-stuff production method, and this method makes bacterium spherical diameter and quality reach directly edible requirement by the classification cultured continuously, can realize that the industrialization of edible mushrooms is produced fast.For this reason, the present invention also will provide a kind of special-purpose air-lift culturing tank of producing bacterium ball food.
In order to solve the problems of the technologies described above, bacterium ball food-stuff production method of the present invention is as follows:
A kind of bacterium ball food-stuff production method is undertaken by following processing step:
The preparation of a, substratum:
Method routinely prepares substratum, and it is standby to pump into storage tank after sterilization is cooled to 25～26 ℃;
The preparation of b, liquid spawn:
In triangular flask, load onto and state the good substratum of sterilising treatment, get slant strains, under aseptic technique, slant strains is inserted in the triangular flask, triangular flask is put into the constant temperature shaking table to be cultivated, culture condition: 120 rev/mins, 26～28 ℃ of temperature were cultivated 3～7 days, treated that changing seed tank culture over to after the bacterium ball is grown makes liquid seeds;
The cultivation of c, bacterium ball:
Substratum in the storage tank is pumped into first step air-lift culturing tank, and the inoculum size by 0.5～10% inserts liquid spawn in the jar, and every cubic metre of nutrient solution is by 0.5～4m 3/ minute speed feed sterile air, temperature is controlled at 20 ℃～34 ℃ cultivates; Occur when there are a large amount of bacterium balls in the discharge gate zone, and when reaching more than 3 millimeters diameter, promptly begin to carry out cultured continuously; Promptly open feed valve, to this air-lift culturing tank input fresh culture, open blow-off valve simultaneously and will enter the cultivation of next stage air-lift culturing tank, stay simple air-lift culturing tank continuation cultivation less than the bacterium ball and the mycelia of 3 mm dias greater than the bacterium ball of 3 mm dias and the mixture of culture material;
When the growth of bacterium ball meets the requirements of diameter in the next stage air-lift culturing tank, to open blow-off valve and begin to collect product separation, the mixture that maybe will meet the requirements of diameter bacterium ball and culture material is imported more next stage air-lift culturing tank continuation cultivation; So cultivate operation repeatedly, up to turning out the bacterium ball that reaches the requirement specification through secondary or the air-lift culturing tank more than the secondary;
D, classification:
Classification in the classifier of sizing screen is equipped with in the culture material input that secondary or the above air-lift culturing tank of secondary are emitted, and the big bacterium ball that reaches specification requirement carries out the classification vacuum packaging, becomes product; The little bacterium ball input one-level air-lift culturing tank circulation that does not reach specification requirement is cultivated; The screening mixture of undersized little bacterium ball, mycelia and culture material returns dosing vessel.
In order to realize bacterium ball food-stuff production method, the present invention has adopted a kind of air-lift culturing tank of producing bacterium ball food, it comprises tank body, opening for feed, discharge gate, opening for feed is above tank body, discharge gate is provided with from discharge gate in the tank interior bottom and begins acclivitous classification sieve plate below tank body; Venting port is established at the top of tank body, and the middle part is provided with the airway of setting in jar; The lower end of airway is over against the air-distributor of jar inner bottom part, and this air-distributor connects a jar outer pneumatic pump by the inlet pipe of jar bottom.
In order to realize bacterium ball food-stuff production method, the present invention can also adopt second kind of air-lift culturing tank of producing bacterium ball food, it comprises tank body, opening for feed, discharge gate, opening for feed is above tank body, discharge gate is provided with from discharge gate in the tank interior bottom and begins acclivitous classification sieve plate below tank body; Venting port is established at the top of tank body, the tank body next door is provided with the airway of setting, the upper and lower end of airway all has the gas-liquid channel that communicates with tank body, in the lower end of airway air-distributor is arranged, and this air-distributor connects a jar outer pneumatic pump by the inlet pipe of airway bottom.
The present invention adopts natural components such as starch, bean powder, fish meal and sucrose to make the bacterium ball that raw material is produced edible meat tissue.Adopt the automatic classification technology of continuously fermenting with short production cycle on the technology, only need 2-4 days to bacterium ball product from the cultivation of bacterium ball; Culturing process material reusable edible does not have any pollutant emission, and the cost of producing bacterial classification reduces greatly, is beneficial to batch production production, has remarkable economic efficiency and social benefit.Particularly the present invention adopts special-purpose air-lift culturing tank to cultivate liquid spawn, adopts air-flow to drive liquid and makes culture at the jar internal recycle, and the mycelia fracture is few, and the classification sieve plate of inclination can carry out automatic classification with the bacterium ball of different sizes, and bacterium sphere volume size is consistent.Compared with prior art, simple in structure having saved has Electric heating bar and control device in the complicated tank body.
Description of drawings
Below in conjunction with drawings and embodiments the present invention is described in further details;
Fig. 1 is a process flow sheet of the present invention;
Fig. 2 is first structural representation of air-lift culturing tank of the present invention;
Fig. 3 is second structural representation of air-lift culturing tank of the present invention.
Embodiment
Embodiment 1
Be a kind of golden mushroom ball food-stuff production method:
The preparation of a, substratum:
1. get flour 2%; Sucrose 1%; Fish meal 0.5%; Water 97.5% is put into dosing vessel and is mixed, and adjusts pH value 5.5～6.5, stirs;
2. sterilising treatment: get above-mentioned mixed solution filtrate and import 2m 3In the sterilization tank, logical high-temperature steam makes the substratum temperature bring up to 130 ℃ in tank body, is incubated 30 minutes; It is standby to pump into storage tank after being cooled to 25 ℃;
The preparation of b, liquid spawn:
Preparing specification is the triangular flask of 1000ml, in dress 200m sterilising treatment and be cooled to the substratum of room temperature, get slant strains, under aseptic technique, the needle mushroom slant strains is linked in the triangular flask, triangular flask is put into the constant temperature shaking table cultivate, culture condition is: 120 times/minute, and 28 ℃ of temperature, cultivated 3 days, and treated that changing seed tank culture over to after the bacterium ball is grown made liquid seeds;
The cultivation of c, bacterium ball:
Present embodiment adopts three grades of cultivations, and employed one-level air-lift culturing tank is 500L, and secondary and three grades of air-lift culturing tanks are 1m 3
The air-lift culturing tank of one-level 500L wherein, see Fig. 2, comprise tank body 1, opening for feed 4, discharge gate 5, opening for feed 4 is above tank body 1, discharge gate 5 is below tank body 1, and venting port 8 is established at the top of tank body 1, and the middle part is provided with the airway 2 of setting in jar, the lower end of airway 2 is over against the air-distributor 7 of jar inner bottom part, and this air-distributor 7 connects a jar outer pneumatic pump by the inlet pipe 6 of jar bottom; Be provided with from discharge gate 5 in tank body 1 inner lower and begin acclivitous classification sieve plate 3, angle α=20 of its vergence direction and tank skin °.
Secondary 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=45 of its classification sieve plate 3 vergence directions and tank skin °.
Three grades of 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=85 of its classification sieve plate 3 vergence directions and tank skin °.
The substratum that sterilization is good pumps into one-level air-lift culturing tank 400L, secondary air-lift culturing tank 700L, three grades of air-lift culturing tank 600L; The 2L liquid spawn is inserted the one-level air-lift culturing tank; Press 0.25m 3/ minute speed feed sterile air, temperature is controlled at 20 ℃ cultivates; Cultivating after 24 hours the discharge gate zone has a large amount of bacterium balls to occur, and promptly begins to carry out cultured continuously; Open feed valve, import fresh culture with 40L/ hour speed to the one-level air-lift culturing tank, open blow-off valve simultaneously with bacterium ball and culture material input secondary air-lift culturing tank, when culture material reaches the 800L liquid level in the secondary air-lift culturing tank, open blow-off valve with three grades of air-lift culturing tanks of culture material input, make the liquid level of secondary air-lift culturing tank remain on the 800L liquid level, when secondary air-lift culturing tank discharge gate zone has a large amount of bacterium balls to occur, open feed valve and import fresh culture to the secondary air-lift culturing tank with 40L/ hour speed; When culture material reaches the 800L liquid level in three grades of air-lift culturing tanks, open blow-off valve and make three grades of air-lift culturing tanks remain on the 800L liquid level discharge of part culture material; When occurring a large amount of bacterium ball in the culture material of discharging, begin to collect product separation;
D, classification:
Classification in the classifier of 10mm and 5mm two-stage sizing screen is equipped with in the culture material input that three grades of air-lift culturing tanks are emitted, and carries out vacuum packaging greater than the big bacterium ball of 10mm, becomes product; The bacterium ball of size input one-level air-lift culturing tank continues to cultivate between 5mm to 10mm; The screening mixture of little bacterium ball, mycelia and the culture material of 5mm returns dosing vessel.
Embodiment 2
Be a kind of oyster mushroom ball food-stuff production method:
The preparation of a, substratum:
1. get flour 2%; Sucrose 1.5%; Fish meal 0.5%; Water 97% is put into dosing vessel and is mixed, and adjusts pH value 5.5～6.5, stirs;
2. sterilising treatment: get above-mentioned mixed solution filtrate and import 2m 3In the sterilization tank, logical high-temperature steam makes the substratum temperature bring up to 130 ℃ in tank body, is incubated 30 minutes; It is standby to pump into storage tank after being cooled to 26 ℃;
The preparation of b, liquid spawn:
Preparing specification is the triangular flask of 1000ml, in dress 200m sterilising treatment and be cooled to the substratum of room temperature, get slant strains, under aseptic technique, the Pleurotus geesteranus slant strains is linked in the triangular flask, triangular flask is put into the constant temperature shaking table cultivate, culture condition is: 120 times/minute, and 27 ℃ of temperature, cultivated 6 days, and treated that changing seed tank culture over to after the bacterium ball is grown made liquid seeds;
The cultivation of c, bacterium ball:
Present embodiment adopts three grades of cultivations, and employed one-level air-lift culturing tank is 500L, and secondary and three grades of air-lift culturing tanks are 1m 3
The air-lift culturing tank of one-level 500L wherein, see Fig. 2, comprise tank body 1, opening for feed 4, discharge gate 5, opening for feed 4 is above tank body 1, discharge gate 5 is below tank body 1, and venting port 8 is established at the top of tank body 1, and the middle part is provided with the airway 2 of setting in jar, the lower end of airway 2 is over against the air-distributor 7 of jar inner bottom part, and this air-distributor 7 connects a jar outer pneumatic pump by the inlet pipe 6 of jar bottom; Be provided with from discharge gate 5 in tank body 1 inner lower and begin acclivitous classification sieve plate 3, angle α=40 of its vergence direction and tank skin °.
Secondary 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=60 of its classification sieve plate 3 vergence directions and tank skin °.
Three grades of 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=85 of its classification sieve plate 3 vergence directions and tank skin °.
The substratum that sterilization is good pumps into one-level air-lift culturing tank 400L, secondary air-lift culturing tank 700L, three grades of air-lift culturing tank 600L; The 12L liquid spawn is inserted the one-level air-lift culturing tank; Press 0.8m 3/ minute speed feed sterile air, temperature is controlled at 25 ℃ cultivates; Cultivating after 24 hours the discharge gate zone has a large amount of bacterium balls to occur, and promptly begins to carry out cultured continuously; Open feed valve, import fresh culture with 40L/ hour speed to the one-level air-lift culturing tank, open blow-off valve simultaneously with bacterium ball and culture material input secondary air-lift culturing tank, when culture material reaches the 800L liquid level in the secondary air-lift culturing tank, open blow-off valve with three grades of air-lift culturing tanks of culture material input, make the liquid level of secondary air-lift culturing tank remain on the 800L liquid level, when secondary air-lift culturing tank discharge gate zone has a large amount of bacterium balls to occur, open feed valve and import fresh culture to the secondary air-lift culturing tank with 40L/ hour speed; When culture material reaches the 800L liquid level in three grades of air-lift culturing tanks, open blow-off valve and make three grades of air-lift culturing tanks remain on the 800L liquid level discharge of part culture material; When occurring a large amount of bacterium ball in the culture material of discharging, begin to collect product separation;
D, classification:
This step is with embodiment 1.
Embodiment 3
Be a kind of mythic fungus bacterium ball food-stuff production method:
The preparation of a, substratum:
1. get raw material by Semen Maydis powder 2%; Sucrose 1%; Yeast powder 0.1%; Water 97.9% obtaining liq substratum;
2. sterilising treatment: get above-mentioned mixed solution filtrate and import 2m 3In the sterilization tank, logical high-temperature steam makes the substratum temperature bring up to 130 ℃ in tank body, is incubated 30 minutes; It is standby to pump into storage tank after being cooled to 26 ℃;
The preparation of b, liquid spawn:
Preparing specification is the triangular flask of 1000ml, in dress 200m sterilising treatment and be cooled to the substratum of room temperature, get fresh slant strains, under aseptic technique, the Pleurotus geesteranus slant strains is linked in the triangular flask, triangular flask is put into the constant temperature shaking table cultivate, culture condition is: 120 times/minute, and 27 ℃ of temperature, cultivated 4 days, and treated that changing seed tank culture over to after the bacterium ball is grown made liquid seeds;
The cultivation of c, bacterium ball:
Present embodiment adopts three grades of cultivations, and employed one-level air-lift culturing tank is 500L, and secondary and three grades of air-lift culturing tanks are 1m 3
The air-lift culturing tank of 500L wherein, comprise tank body 1, opening for feed 4, discharge gate 5, opening for feed 4 is above tank body 1, discharge gate 5 is below tank body 1, and venting port 8 is established at the top of tank body 1, and tank body 1 next door is provided with the airway 2 of setting, there is the gas-liquid channel 9 that communicates with tank body 1 upper end of airway 2, there is the gas-liquid channel 10 that communicates with tank body 1 lower end, in the lower end of airway 2 air-distributor 7 is arranged, and this air-distributor 7 connects a jar outer pneumatic pump by the inlet pipe 6 of airway bottom; Be provided with from discharge gate 5 in tank body 1 inner lower and begin acclivitous classification sieve plate 3, angle α=30 of its vergence direction and tank skin °.
Secondary 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=45 of its classification sieve plate 3 vergence directions and tank skin °.
Three grades of 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=85 of its classification sieve plate 3 vergence directions and tank skin °.
The substratum that sterilization is good pumps into one-level air-lift culturing tank 400L, secondary air-lift culturing tank 700L, three grades of air-lift culturing tank 600L; Inoculum size by 5% is that the 20L liquid spawn is inserted the one-level air-lift culturing tank; Press 1.2m 3/ minute feeding sterile air is controlled at 30 ℃ with temperature and cultivates; Cultivating after 24 hours the discharge gate zone has a large amount of bacterium balls to occur, and promptly begins to carry out cultured continuously; Open feed valve, import fresh culture with 40L/ hour speed to the one-level air-lift culturing tank, open blow-off valve simultaneously with bacterium ball and culture material input secondary air-lift culturing tank, when culture material reaches the 800L liquid level in the secondary air-lift culturing tank, open blow-off valve with three grades of air-lift culturing tanks of culture material input, make the liquid level of secondary air-lift culturing tank remain on the 800L liquid level, when secondary air-lift culturing tank discharge gate zone has a large amount of bacterium balls to occur, open feed valve and import fresh culture to the secondary air-lift culturing tank with 40L/ hour speed; When culture material reaches the 800L liquid level in three grades of air-lift culturing tanks, open blow-off valve and make three grades of air-lift culturing tanks remain on the 800L liquid level discharge of part culture material; When occurring a large amount of bacterium ball in the culture material of discharging, begin to collect product separation;
D, classification:
This step is with embodiment 1.
Embodiment 4
Be a kind of straw mushroom bacterium ball food-stuff production method:
The preparation of a, substratum:
1. get raw material by Semen Maydis powder 2%; Sucrose 1%; Yeast powder 0.2%; Water 97.8% obtaining liq substratum;
2. sterilising treatment: get above-mentioned mixed solution filtrate and import 2m 3In the sterilization tank, logical high-temperature steam makes the substratum temperature bring up to 130 ℃ in tank body, is incubated 30 minutes; It is standby to pump into storage tank after being cooled to 25 ℃;
The preparation of b, liquid spawn:
Preparing specification is the triangular flask of 1000ml, in dress 200m sterilising treatment and be cooled to the substratum of room temperature, get fresh slant strains, under aseptic technique, the straw mushroom slant strains is linked in the triangular flask, triangular flask is put into the constant temperature shaking table cultivate, culture condition is: 120 times/minute, and 26 ℃ of temperature, cultivated 7 days, and treated that changing seed tank culture over to after the bacterium ball is grown made liquid seeds;
The cultivation of c, bacterium ball:
Present embodiment adopts three grades of cultivations, and employed one-level air-lift culturing tank is 500L, and secondary and three grades of air-lift culturing tanks are 1m 3
The air-lift culturing tank of 500L wherein, comprise tank body 1, opening for feed 4, discharge gate 5, opening for feed 4 is above tank body 1, discharge gate 5 is below tank body 1, and venting port 8 is established at the top of tank body 1, and tank body 1 next door is provided with the airway 2 of setting, there is the gas-liquid channel 9 that communicates with tank body 1 upper end of airway 2, there is the gas-liquid channel 10 that communicates with tank body 1 lower end, in the lower end of airway 2 air-distributor 7 is arranged, and this air-distributor 7 connects a jar outer pneumatic pump by the inlet pipe 6 of airway bottom; Be provided with from discharge gate 5 in tank body 1 inner lower and begin acclivitous classification sieve plate 3, angle α=40 of its vergence direction and tank skin °.
Secondary 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=65 of its classification sieve plate 3 vergence directions and tank skin °.
Three grades of 1m wherein 3Air-lift culturing tank and the air-lift culturing tank of 500L similar, different is: angle α=85 of its classification sieve plate 3 vergence directions and tank skin °.
The substratum that sterilization is good pumps into one-level air-lift culturing tank 400L, secondary air-lift culturing tank 700L, three grades of air-lift culturing tank 600L; Inoculum size by 10% is that the 40L liquid spawn is inserted the one-level air-lift culturing tank; Press 2m 3/ minute feeding sterile air is controlled at 34 ℃ with temperature and cultivates; Cultivating after 24 hours the discharge gate zone has a large amount of bacterium balls to occur, and promptly begins to carry out cultured continuously; Open feed valve, import fresh culture with 40L/ hour speed to the one-level air-lift culturing tank, open blow-off valve simultaneously with bacterium ball and culture material input secondary air-lift culturing tank, when culture material reaches the 800L liquid level in the secondary air-lift culturing tank, open blow-off valve with three grades of air-lift culturing tanks of culture material input, make the liquid level of secondary air-lift culturing tank remain on the 800L liquid level, when secondary air-lift culturing tank discharge gate zone has a large amount of bacterium balls to occur, open feed valve and import fresh culture to the secondary air-lift culturing tank with 40L/ hour speed; When culture material reaches the 800L liquid level in three grades of air-lift culturing tanks, open blow-off valve and make three grades of air-lift culturing tanks remain on the 800L liquid level discharge of part culture material; When occurring a large amount of bacterium ball in the culture material of discharging, begin to collect product separation;
D, classification:
This step is with embodiment 1.

Claims (3)

1, a kind of bacterium ball food-stuff production method is characterized in that:
The preparation of a, substratum:
Method routinely prepares substratum, and it is standby to pump into storage tank after sterilization is cooled to 25～26 ℃;
The preparation of b, liquid spawn:
In triangular flask, load onto and state the good substratum of sterilising treatment, get slant strains, under aseptic technique, slant strains is inserted in the triangular flask, triangular flask is put into the constant temperature shaking table to be cultivated, culture condition: 120 rev/mins, 26～28 ℃ of temperature were cultivated 3～7 days, treated that changing seed tank culture over to after the bacterium ball is grown makes liquid seeds;
The cultivation of c, bacterium ball:
Substratum in the storage tank is pumped into first step air-lift culturing tank, and the inoculum size by 0.5～10% inserts liquid spawn in the jar, and every cubic metre of nutrient solution is by 0.5～4m 3/ minute speed feed sterile air, temperature is controlled at 20 ℃～34 ℃ cultivates; Occur when there are a large amount of bacterium balls in the discharge gate zone, and when reaching more than 3 millimeters diameter, promptly begin to carry out cultured continuously; Promptly open feed valve, to this air-lift culturing tank input fresh culture, open blow-off valve simultaneously and will enter the cultivation of next stage air-lift culturing tank, stay simple air-lift culturing tank continuation cultivation less than the bacterium ball and the mycelia of 3 mm dias greater than the bacterium ball of 3 mm dias and the mixture of culture material;
When the growth of bacterium ball meets the requirements of diameter in the next stage air-lift culturing tank, to open blow-off valve and begin to collect product separation, the mixture that maybe will meet the requirements of diameter bacterium ball and culture material is imported more next stage air-lift culturing tank continuation cultivation; So cultivate operation repeatedly, up to turning out the bacterium ball that reaches the requirement specification through secondary or the air-lift culturing tank more than the secondary;
D, classification:
Classification in the classifier of sizing screen is equipped with in the culture material input that secondary or the above air-lift culturing tank of secondary are emitted, and the big bacterium ball that reaches specification requirement carries out the classification vacuum packaging, becomes product; The little bacterium ball input one-level air-lift culturing tank circulation that does not reach specification requirement is cultivated; The screening mixture of undersized little bacterium ball, mycelia and culture material returns dosing vessel.
2, a kind of air-lift culturing tank of producing bacterium ball food, comprise tank body (1), opening for feed (4), discharge gate (5), opening for feed (4) is in the top of tank body (1), discharge gate (5) is in the below of tank body (1), venting port (8) is established at the top of tank body (1), the middle part is provided with the airway (2) of setting in jar, and the lower end of airway (2) is over against the air-distributor (7) of jar inner bottom part, and this air-distributor (7) connects a jar outer pneumatic pump by the inlet pipe (6) of jar bottom; It is characterized in that: be provided with from discharge gate (5) in tank body (1) inner lower and begin acclivitous classification sieve plate (3), angle α=20 of its vergence direction and tank skin °～85 °.
3, a kind of air-lift culturing tank of producing bacterium ball food, comprise tank body (1), opening for feed (4), discharge gate (5), opening for feed (4) is in the top of tank body (1), discharge gate (5) is in the below of tank body (1), venting port (8) is established at the top of tank body (1), tank body (1) next door is provided with the airway (2) of setting, the upper and lower end of airway (2) all has the gas-liquid channel (9,10) that communicates with tank body (1), in the lower end of airway (2) air-distributor (7) is arranged, this air-distributor (7) connects a jar outer pneumatic pump by the inlet pipe (6) of airway bottom; It is characterized in that: be provided with from discharge gate (5) in tank body (1) inner lower and begin acclivitous classification sieve plate (3), angle α=20 of its vergence direction and tank skin °～85 °.
CNB2006101368035A 2006-11-30 2006-11-30 Fungus ball food production method and air-lift culturing tank for producing same Expired - Fee Related CN100497588C (en)

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