CN100473331C - Microelectrode for recording laboratory animal visual sense electrophysiological reaction - Google Patents
Microelectrode for recording laboratory animal visual sense electrophysiological reaction Download PDFInfo
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- CN100473331C CN100473331C CNB2006100305113A CN200610030511A CN100473331C CN 100473331 C CN100473331 C CN 100473331C CN B2006100305113 A CNB2006100305113 A CN B2006100305113A CN 200610030511 A CN200610030511 A CN 200610030511A CN 100473331 C CN100473331 C CN 100473331C
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Abstract
The invention discloses a minimized electrode in the physiological checking element domain, which consists of recording electrode and or reference electrode, wherein the recording electrode is composed of conductive electrode rod and electrode on one end of conductive rod; the electrode is ring-shaped metal corneal contacting electrode; or the recording electrode and or reference electrode is composed of insulating conductive electrode rod and electrode on one end of rod conduct; the electrode is needle-shaped metal electrode.
Description
Technical field
The invention belongs to the physiological test instrument field, relate in particular to a kind of device that is used to measure the animal organism signal of telecommunication.
Background technology
Visual System Electrophysiology Technique is a unique objective visual function detection method commonly used on the clinical ophthalmology, and since clinical practice over half a century, its generation all be unable to do without this basis of zoopery with development.
The non-invasive of Visual System Electrophysiology Technique, objectivity, can be deep into cellular and molecular level under the specific condition, and to retina, look the layering location and the accuracy of road lesion detection, make this technology clinical ophthalmology in addition with the relevant crossing research of neural section office in extremely important and irreplaceable effect is arranged.
Visual System Electrophysiology Technique is advanced by leaps and bounds over nearly 10 years, has produced a lot of new techniques, and as the multifocal visual electrophysiology technology, different stimulated figure, spatio-temporal frequency, color etc. can be brought how valuable information to clinical diagnosis and treatment; Developing rapidly of ophthalmology scientific research also impels this technology to be able to continuous expansion, thereby more urgent to the needs of the research of zoopery; Clinical with the scientific research propelling, must be in conjunction with zooperal research, the detection of animal visual electrophysiology is the considerable ingredient in the ophthalmology scientific research.
Along with the foundation of laboratory animal disease model, people also more and more pay attention to the record and the application of animal visual electrophysiology.
Recording electrode, reference electrode and ground electrode are separately positioned on the eye of laboratory animal, near the eye socket and pillow (tail) portion, use suitable recording parameters, can record the corresponding visual electrophysiology response waveform of laboratory animal.
The electrophysiological record of laboratory animal visual needs three essential conditions: unified equipment, unified detected parameters and unified electrode.Now, the former two can and provide by corresponding electric physiological apparatus manufacturing firm's production, aims at the test electrode that animal is used but lack always.
The eighties in last century, wire electrodes such as beginning applied metal silk or carbon fibre serve as recording electrode in the external experiment.Because wire electrode settles in animal eye, fixing inconvenient, drift easily, disturbance records makes the waveform that records repeated relatively poor and amplitude is little, thereby directly has influence on the electrophysiological extensive use of laboratory animal visual.
Simultaneously, since cornea size, the radian difference of animal eye, the eyelid difference, and the shape of recording electrode and the difference of processing technology all can have influence on the record result to a great extent.
In addition, the dedicated reference electrode product that never is fit to the test of animal visual electrophysiology now, normally adopt the human reference electrode to substitute in the actual experiment temporarily, because the human reference electrode is a kind of sucked type electrode, for the small-sized laboratory rodent (as testing) that body surface length has more hair with mouse or rabbit etc., its fixing difficulty, and because it can not closely contact with the skin of animal, can bring such as fixing inconvenient, contact bad, problems such as the big and measurement result deviation of contact resistance is big, the waveform repeatability that has also caused recording is relatively poor and amplitude is little, equally directly has influence on the electrophysiological extensive use of laboratory animal visual.
Summary of the invention
Technical problem to be solved by this invention provides a kind of microelectrode that is used for the recording laboratory animal visual electrophysiologic response, and its location is convenient, accurate, and is easily fixing, and wave recording is stable, the signal to noise ratio height, and repeatability is well, and is cheap for manufacturing cost.
Technical scheme of the present invention is: a kind of microelectrode that is used for the recording laboratory animal visual electrophysiologic response is provided, comprise recording electrode and/or reference electrode, it is characterized in that: its recording electrode is made of with the electrode that is arranged on one of body of rod conductor end the conductive electrode body of rod of external insulating barrier; Its described electrode is a metal ring cornea contact electrode.
Above-mentioned ring-type cornea contact electrode is the individual pen contact electrode, and the eye's cornea size of individual pen contact electrode and measurand is complementary.
Perhaps, the two circle contact electrodes of above-mentioned ring-type cornea contact electrode for constituting by outer ring electrode and inner ring electrode, its inside and outside circle electrode is connected with the electrode body of rod after linking to each other, the eyelid size of circle electrode and measurand is complementary outside two circle contact electrodes, and the eye's cornea size of its inner ring electrode and measurand is complementary.
Further, the outer ring electrode of its pair circle contact electrode and inner ring electrode are arranged in the same plane or are arranged in two planes that are parallel to each other.
Above-mentioned single, double circle contact electrode is circle, ellipse, sub-circular or approximate ellipsoidal.
Above-mentioned single, double circle contact electrode surface is provided with gold-plated or silver coating.
The present invention also provides the another kind of microelectrode that is used for the recording laboratory animal visual electrophysiologic response, comprise recording electrode and/or reference electrode, it is characterized in that: its recording electrode and/or reference electrode are made of with the electrode that is arranged on one of body of rod conductor end the conductive electrode body of rod of external insulating barrier; Its described electrode is the metal needle electrode.
Compared with the prior art, advantage of the present invention is:
1. recording electrode adopts circulus, and the location is convenient, accurate, can adapt to different tested objects;
2. adopt gold-plated or the silver coating structure, draw signal stabilization, the signal to noise ratio height, cheap for manufacturing cost;
3. adopt two coil structures, guaranteed that contact is good, make and draw signal stabilization, the signal to noise ratio height, repeating of measurement result is functional;
4. recording electrode and/or reference electrode adopt the metal needle electrode, and its location is convenient, accurate, and is easily fixing, guarantee contact well, and wave recording is stable, the signal to noise ratio height, and the waveform that records repeatability is good;
5. be specially adapted to the detection and the record of the visual electrophysiology response waveform of small-sized rodent.
Description of drawings
Fig. 1 is the structural representation of the present invention's annular contact electrode;
Fig. 2 is the structural representation of the present invention's annular contact electrode embodiment;
Fig. 3 is the structural representation of needle electrode of the present invention;
Fig. 4 is the ERG oscillogram of the measured rabbit eyes of the electrode that adopts the present invention;
Fig. 5 is the ERG oscillogram of the measured rat eyes of the electrode that adopts the present invention;
Fig. 6 is the FVEP oscillogram of the measured rat eyes of the electrode that adopts the present invention.
1 is electrode stem among the figure, and 2 is the ring-type contact electrode, and 3 is the outer ring electrode, and 4 is the inner ring electrode, and 5 is needle electrode.
The specific embodiment
Among Fig. 1, the technical program is provided with ring-type cornea contact electrode 2 at an end of the body of rod 1 conductor.
Wherein, ring-type cornea contact electrode is the individual pen contact electrode, and the eye's cornea size of individual pen contact electrode and measurand is complementary.
Individual pen contact electrode surface is provided with gold-plated or silver coating.
The individual pen contact electrode is circular or oval.
Among Fig. 2, the two circle contact electrodes of ring-type cornea contact electrode for constituting by outer ring electrode 3 and inner ring electrode 4, its inside and outside circle electrode is connected with the electrode body of rod 1 after linking to each other, the eyelid size of circle electrode and measurand is complementary outside two circle contact electrodes, and the eye's cornea size of its inner ring electrode and measurand is complementary.
The outer ring electrode of above-mentioned two circle contact electrodes and inner ring electrode are arranged in the same plane or are arranged in two planes that are parallel to each other.
All the other same Fig. 1.
Because of the eyeball of the animal of variety classes varies in size, leading electrode adopts the version of ring electrode, both can adapt to different experimental animal kinds by the diameter of regulating ring electrode, can guarantee again that the location of electrode is convenient, accurately; Because adopt gold-plated or silver coating structure, electrode impedance is less, draws signal stabilization, the signal to noise ratio height, the while has also been reduced the manufacturing cost of electrode; In addition, adopt two coil structures, put into animal eyeball easily and fix, its outer ring had both increased contact area, can play fixation again, and inner ring can play the measurement effect, the contact that has guaranteed test electrode is good, makes to draw signal stabilization, and repeating of measurement result is functional.
Among Fig. 3, recording electrode and/or reference electrode are made of the electrode body of rod 1 and the electrode that is arranged on one of body of rod conductor end; Its electrode is a metal needle electrode 5.
Adopt the metal needle electrode, can directly it be thrust the subcutaneous of laboratory animal, its location is convenient, accurate, and is easily fixing, guaranteed that the contact of test electrode is good, makes wave recording stable, the signal to noise ratio height, and the waveform that records repeatability is well.
Embodiment 1:
Recording electrode adopts ring-type cornea contact electrode, and reference electrode, ground electrode adopt needle electrode, and laboratory animal is a rabbit.
Drip methylcellulose (or ofloxacin eye ointment) on rabbit eye's cornea surface, two circle formula contact electrodes that the inside/outside electrode diameter is respectively 3mm/5mm are placed in anterior corneal surface anesthesia back, reference electrode, ground electrode needle electrode thrust homonymy ear and head respectively.
Adopt above-mentioned microelectrode, cooperate with U.S. LKC UTAS-2000 type electrophysiology instrument, dual pathways record ERG obtains wave recording as shown in Figure 4.
As can be seen from the figure, this noiseless clutter (promptly not having steep peak) of a in the shown wavy curve → b segment base, wavy curve is more smooth, and it is better relatively that the wave mode that same animal records repeats performance, embodied the technical program and drawn signal stabilization, the advantage that signal to noise ratio is high.
Embodiment 2:
Recording electrode adopts ring-type cornea contact electrode, and reference electrode, ground electrode adopt needle electrode, and laboratory animal is BN (BrownNorway) rat.
Recording electrode adopts two circle formula contact electrodes of 0.8mm/1.5mm, directly puts into eyeball behind the anesthetized animal, and drip with normal saline or methylcellulose on the surface, and reference electrode, ground electrode needle electrode thrust homonymy ear and head respectively.
Adopt above-mentioned microelectrode, cooperate with U.S. LKC UTAS-2000 type electrophysiology instrument, dual pathways record ERG obtains wave recording as shown in Figure 5.
As can be seen from the figure, the N1 in the shown wavy curve → noiseless clutter of O4 section, wavy curve is more smooth, has good diagnosis and estimate to be worth.
All the other are with embodiment 1.
Embodiment 3:
It is the metal needle electrode of needle point that recording electrode, reference electrode and ground electrode adopt injection 1ml stainless pin, and laboratory animal is the Wistar rat.
It is subcutaneous that recording electrode thrusts two ear lines, and reference electrode, ground electrode thrust homonymy ear and head respectively.
Obtain the FVEP wave recording as shown in Figure 6.
As can be seen from the figure, shown wavy curve does not have noise jamming, waveform stabilization, and it is convenient, accurately to have embodied the technical program location, and easily fixing, contact is good, and wave recording is stable, the good advantage of waveform repeatability that records.
All the other are with embodiment 1 or 2.
In the experiment, adopt above-mentioned microelectrode, the electric physiological conditions of eye to rat, rabbit, Cavia porcellus, mice is tested, all can record ERG (Electroretinogram very easily, electroretinogram) and VEP (Visual Evoked Potential, visual evoked potential), the waveform stabilization that is recorded, repeating of measurement result is functional.
In addition, the how burnt electroretinogram record of laboratory animal has also been carried out preliminary observation, conclusion is to measure and to record the visual electrophysiology waveform of laboratory animal with above-mentioned electrode, and the wave mode repetition performance that same animal records is relatively good.
As seen, development of this electrode and exploitation are the research and the scientific research of animal visual electrophysiology reaction, have established good detection basis.
The present invention can be widely used in the field tests of the visual electrophysiology reaction of multiple animal (especially small-sized rodent).
Claims (3)
1. recording electrode that is used for the recording laboratory animal visual electrophysiologic response is characterized in that:
Its recording electrode is made of with the electrode that is arranged on one of body of rod conductor end the conductive electrode body of rod of external insulating barrier;
Its described electrode is two circle formula metal ring cornea contact electrodes;
Described pair of circle formula metal ring cornea contact electrode is made of outer ring electrode and inner ring electrode, and its inside and outside circle electrode is connected with the electrode body of rod after linking to each other.
2. according to the described recording electrode that is used for the recording laboratory animal visual electrophysiologic response of claim 1, it is characterized in that the eyelid size of enclosing electrode and measurand outside the described pair of circle formula metal ring cornea contact electrode is complementary, the eye's cornea size of its inner ring electrode and measurand is complementary.
3. according to the described recording electrode that is used for the recording laboratory animal visual electrophysiologic response of claim 1, it is characterized in that the outer ring electrode of described two circle formula metal ring cornea contact electrodes and inner ring electrode are arranged in the same plane or are arranged in two planes that are parallel to each other.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006100305113A CN100473331C (en) | 2006-08-29 | 2006-08-29 | Microelectrode for recording laboratory animal visual sense electrophysiological reaction |
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| CNB2006100305113A CN100473331C (en) | 2006-08-29 | 2006-08-29 | Microelectrode for recording laboratory animal visual sense electrophysiological reaction |
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| CN1907213A CN1907213A (en) | 2007-02-07 |
| CN100473331C true CN100473331C (en) | 2009-04-01 |
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| CN109700427A (en) * | 2019-02-28 | 2019-05-03 | 中国人民解放军第306医院 | A kind of cornea contact electrode and working method that can measure pupil size simultaneously |
| CN109700428A (en) * | 2019-02-28 | 2019-05-03 | 中国人民解放军第306医院 | A kind of cornea contact electrode and working method of built-in eye gaze tracking system |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4874237A (en) * | 1987-05-07 | 1989-10-17 | Lions Eye Inst. Of Western Australia | Electroretinogram apparatus |
| CN2075501U (en) * | 1990-09-28 | 1991-04-24 | 中国人民解放军白求恩国际和平医院 | Retina electric graph recording electrode |
| CN1060021A (en) * | 1990-09-26 | 1992-04-08 | 中国人民解放军白求恩国际和平医院 | A kind of monitor electrode for visual electrophysiotogic operation |
| US5154174A (en) * | 1990-04-20 | 1992-10-13 | Marko Hawlina | Electrode for electroretinography and method of use |
| CN2626430Y (en) * | 2003-06-30 | 2004-07-21 | 苏州六六视觉科技股份有限公司 | Optical scopic electric physiological cornea contact lens electrode |
| CN2633210Y (en) * | 2003-07-08 | 2004-08-18 | 重庆海威康医疗仪器有限公司 | Plug-in type disposable medical needle electrode |
-
2006
- 2006-08-29 CN CNB2006100305113A patent/CN100473331C/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4874237A (en) * | 1987-05-07 | 1989-10-17 | Lions Eye Inst. Of Western Australia | Electroretinogram apparatus |
| US5154174A (en) * | 1990-04-20 | 1992-10-13 | Marko Hawlina | Electrode for electroretinography and method of use |
| CN1060021A (en) * | 1990-09-26 | 1992-04-08 | 中国人民解放军白求恩国际和平医院 | A kind of monitor electrode for visual electrophysiotogic operation |
| CN2075501U (en) * | 1990-09-28 | 1991-04-24 | 中国人民解放军白求恩国际和平医院 | Retina electric graph recording electrode |
| CN2626430Y (en) * | 2003-06-30 | 2004-07-21 | 苏州六六视觉科技股份有限公司 | Optical scopic electric physiological cornea contact lens electrode |
| CN2633210Y (en) * | 2003-07-08 | 2004-08-18 | 重庆海威康医疗仪器有限公司 | Plug-in type disposable medical needle electrode |
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