CN100462710C - Process for processing waste liquid in box and chemical reaction box using the same process - Google Patents
Process for processing waste liquid in box and chemical reaction box using the same process Download PDFInfo
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- CN100462710C CN100462710C CNB2004100906027A CN200410090602A CN100462710C CN 100462710 C CN100462710 C CN 100462710C CN B2004100906027 A CNB2004100906027 A CN B2004100906027A CN 200410090602 A CN200410090602 A CN 200410090602A CN 100462710 C CN100462710 C CN 100462710C
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- waste liquid
- box
- chemical reaction
- liquid tank
- cartridge
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Abstract
The present invention injects liquid absorbent into waste liquid tank set inside sealed chemical reaction box, in which the liquid absorbent absorbs harmful matters, such as waste liquid containing pathogen or endocrine interfering chemical matter, so as to avoid the outward diffusion of the harmful matters. In addition, the waste liquid tank is provided with port to outside, and the port is sealed with filter to allow gaseous matter to pass through while blocking liquid or solid matter so as to control the inside liquid transfer conveniently.
Description
Invention field
The present invention relates to various cartridge for chemical reaction, as biological diagnosis box that in the genetic diagnosis system, uses or the biosensor cartridge that is used for environment measurement, specifically, relate to and have and to be sealed in the box of closed structure wherein such as objectionable impuritiess such as virus or endocrine interfering chemicalses; Even the invention still further relates under the ruined situation of box, can prevent that also objectionable impurities is to the method for treating waste liquid and the structure of external diffusion in the box; And relate to the liquid that makes in this box and shift easy controlled structure.
Background technology
Recently, developed the international analysis of describing in open of the biological diagnosis box that in the genetic diagnosis system, uses, the biosensor cartridge that is applied to environment measurement and WO 01/013127 with box etc.
In the box of these types, for example in the biosensor cartridge of biological diagnosis box or environment measurement, be necessary to prevent in the sample contained such as objectionable impuritiess such as virus or endocrine interfering chemicalses to external diffusion.
The structure of some box can be enclosed in the box such as the waste liquid the damping fluid of analyzing usefulness.But, even still there is the problem that diffusion will take place as the destroyed objectionable impurities of compartmentalized box for holding assorted fruits and candies in this class box.
Identical with the situation of box, use on the chip of so-called " μ TAS " (micro-aggregate analysis system) on the laboratory (lab-on-a-chip) and chip preparation room (fab-on-a-chip) equally also to exist and need prevent the problem of waste liquid to external diffusion.On laboratory and the chip in the preparation room, the cell of test, laboratory, chemical plant etc. are fabricated on the chip on described chip.
Summary of the invention
To an object of the present invention is in order addressing the above problem, a kind of method that disposes waste liquid in box to be provided, comprise following steps: liquid-absorbant is injected in the waste liquid tank that is arranged in the airtight cartridge for chemical reaction; In described box, absorb these objectionable impuritiess of waste liquid that contain pathogenic bacteria or endocrine interfering chemicals by the described liquid-absorbant in the described waste liquid tank; And with described objectionable impurities gelation, described waste liquid tank is provided with and one or morely is communicated with outside openings, and described opening is by allowing gas permeation but the filtrator sealing that does not allow the hydrophobic nature perforated membrane of liquid or solid material permeance to make.This method can prevent such as the objectionable impuritiess such as waste liquid that contain pathogenic bacteria etc. or endocrine interfering chemicals to external diffusion, and help carrying out the transfer control of internal liquid.
Another object of the present invention is to provide a kind of airtight cartridge for chemical reaction, wherein be formed with and be used for being stored in sample mixes the waste liquid of back formation with reagent or treating fluid waste liquid tank, this waste liquid tank is constructed to be permeable to the receiving fluids absorbing agent and makes the waste liquid gelation that is imported in the waste liquid tank, described liquid-absorbant is used at described waste liquid tank absorption waste liquid with the waste liquid gelation, described waste liquid tank is provided with one or more openings that are communicated with the outside, and described opening is by allowing gas permeation, but the filtrator that the hydrophobic nature perforated membrane that does not allow the liquid or solid material permeance is made sealing.Even thereby under the ruined situation of box, also can prevent objectionable impurities, and help carrying out the transfer control of internal liquid to external diffusion.
Description of drawings
That Fig. 1 shows is an embodiment of cartridge for chemical reaction of the present invention;
Fig. 2 is the cut-open view of box shown in Fig. 1;
That Fig. 3 shows is another embodiment of cartridge for chemical reaction of the present invention;
Fig. 4 is the cut-open view of box shown in Fig. 3.
Embodiment
Introduce the present invention in detail below with reference to accompanying drawing.Fig. 1 for example understands a specific embodiment of the cartridge for chemical reaction according to the present invention.Fig. 1 is the perspective illustration that comprises the flat board member 2 of cartridge for chemical reaction.The flow passage 20 that on flat board member 2, is formed with a plurality of chambers 10 to 16 and connects each chamber.The hole or the groove part of flow passage that no matter are chamber 10 to 16 do not penetrate flat board member 2.
Sample or testing sample (hereinafter referred to as " sample ") are injected in the chamber 10.Will with the reagent or the treating fluid of sample mixed, cleaning fluids etc. suitably are stored in other the chamber 11 to 14.Chamber 15 is reaction chambers, from the sample of chamber 10, from the treating fluid of chamber 11 to 14 etc. by flow passage 20 inputs wherein.Chamber 16 (hereinafter referred to as " waste liquid tank ") stores and waste liquid can be absorbed the also liquid-absorbant 17 of gelation.
The mode of lid with sealing is positioned on the upper surface of the cartridge for chemical reaction that is formed with above-mentioned chamber and flow passage.Fig. 2 is the cut-open view of cartridge for chemical reaction along the intercepting of A-A ' line, and its middle cover 30 is positioned on the cartridge for chemical reaction shown in Figure 1.
Explain the method for operating of this cartridge for chemical reaction now.Essential reagent or treating fluid are injected in the chamber 11 to 14 in advance.After sample being injected in the chamber 10, this sample is transferred in the reaction chamber 15.
Example with the method for sample flood chamber 10 is: in this cartridge for chemical reaction the inlet of being made by elastic body can be set, the pin of syringe is inserted in the described inlet to inject sample.
In addition, can use the transfer techniques such as pressurization or gas boosting method to shift described sample.For example, under the situation of using gases pressurization, a kind of energy sees through such as the gas of air but the perforated membrane that can not see through liquid be used as and cover 30 so that air from the part that is located immediately at 10 tops, chamber, enter into chamber 10 through this perforated membrane, so that sample is shifted out.Same method is applied to other chambers to shift out content wherein.It should be noted that in this case, be necessary to prevent that air from entering or leaving the chamber of not using the gas boosting method, do not leak into the outside so that air can not see through perforated membrane.
Sample, reagent, treating fluid etc. suitably are input in the reaction chamber 15 by this way.To the measurement of reacted reactive material in reaction chamber 15, be to be undertaken by the electrode of imbedding in the reaction chamber (not showing in the drawings) by externally measured device; Perhaps if measure the light that reactive material radiates, then measure by the transparency window (showing in the drawings) that is arranged on reaction chamber 15 upper surfaces by reader (showing in the drawings).
Carry out in box in the process of series of analysis preparation and measurement at the working specification according to each stage, the waste liquid of each self-generating is collected in the waste liquid tank 16 at last under these different occasions.The liquid-absorbant 17 that stores in the waste liquid tank 16 absorbs waste liquids and with the waste liquid gelation, thereby makes can catch objectionable impurities such as the waste liquid that contains pathogenic bacteria or endocrine interfering chemicals safely in the waste liquid tank 16 of described box.Even box is destroyed, because objectionable impurities is by gel, objectionable impurities can not spread yet.The waste liquid gelation of being undertaken by liquid-absorbant 17 has prevented that also waste liquid is back in the reaction chamber 15.
Above-mentioned waste liquid tank 16 is not because it needs the valve of anti-backflow and simple in structure, and helps making box littler.
As explained above, the present invention has following effect:
(1) when handling objectionable impurities in the boxes such as preparation room on laboratory or the chip on the chip of the biological box that contains chip at genetic diagnosis with box, environment measurement usefulness, use μ TAS, if described box is destroyed, can prevent easily that these objectionable impuritiess from diffusing out from box.
(2) can prevent easily that the waste liquid in the waste liquid tank from refluxing.
(3) with regard to the miniaturization of box, this is a kind of effective method for treating waste liquid.
Should be appreciated that the present invention is not strictly limited to the foregoing description.On the contrary, any other conversion or the improvement of doing about the foregoing description do not break away from spirit of the present invention and essence.
For example, as shown in Figure 3, in the end the top of the waste liquid tank 16 in stage is provided with opening, to help the control of internal liquid, prevents that simultaneously internal liquid is diffused into the outside of waste liquid tank 16.The difference of box shown in Figure 3 and Fig. 1, box shown in Figure 2 is that the lid 3 on the box waste liquid tank 16 shown in Figure 3 is provided with and is used to be communicated with outside opening 31.
The hole of this opening comprises the hole of perforation, and the hole of this opening is not limited to one, can be more than one.Described hole does not need to be arranged on a special position, can be located on the more than one position.As shown in Figure 4, be equipped with filtrator 32 on the opening 31, this filtrator is made by the hydrophobic nature perforated membrane that allows gas permeation but do not allow liquid and solid matter to see through.Filtrator 32 is placed in and covers 3 surface, the back side (as shown in the figure) or cover between 3 the surface and bottom surface, so that waste liquid can not let out from opening 31.
If the waste liquid tank of final stage 16 has closed structure, the air in the groove that is necessary to find time or be provided for hole with external communications on groove is so that help the control of the liquid in chamber or the flow passage.Also be provided with above-mentioned opening in the present embodiment.Preventing the leakage of opening part waste liquid, is to unite realization by the waste liquid that prevents of filtrator 32 to the gelation of external diffusion and harmful waste liquid self, very difficult to external diffusion for gel.
In these boxes, not only waste liquid is imported in the waste liquid tank 16, and the gas that has existed in chamber or flow passage also is imported in the waste liquid tank 16.Yet, have only gas to see through and outwards discharge from opening 31 from filtrator 32.Therefore, when applying pressure came transfer liquid, the air pressure in waste liquid tank 16 or other chambers can not raise, thereby had made things convenient for the control of internal liquid.In addition, be used for the pressure that liquid shifts even stop to apply, the liquid in chamber or the flow passage does not have difference owing to air pressure in the box and can not reflux.
It must be circular hole that described opening there is no need, the opening that can have any shape.Opening can also be made up of a plurality of holes or reticulate texture, as long as the institute of any opening is porosely all sealed by filtrator.
Although the opening in the present embodiment forms on lid 3, opening also can form on flat board member 2.
Claims (5)
1. method that disposes waste liquid in box comprises following steps:
Liquid-absorbant is injected in the waste liquid tank that is arranged in the airtight cartridge for chemical reaction;
In described box, absorb these objectionable impuritiess of waste liquid that contain pathogenic bacteria or endocrine interfering chemicals by the described liquid-absorbant in the described waste liquid tank; And
With described objectionable impurities gelation,
Described waste liquid tank is provided with and one or morely is communicated with outside openings, and described opening is by allowing gas permeation but the filtrator sealing that does not allow the hydrophobic nature perforated membrane of liquid or solid material permeance to make.
2. the method that disposes waste liquid in box according to claim 1, wherein said cartridge for chemical reaction are preparation rooms on laboratory or the chip on the box that is used for genetic diagnosis, the biological box that contains chip that is used for environment measurement or the chip that uses μ TAS.
3. airtight cartridge for chemical reaction, wherein be formed with and be used for being stored in sample mixes the waste liquid of back formation with reagent or treating fluid waste liquid tank, this waste liquid tank is constructed to be permeable to the receiving fluids absorbing agent and makes the waste liquid gelation that is imported in the waste liquid tank, described liquid-absorbant is used at described waste liquid tank absorption waste liquid with the waste liquid gelation
Described waste liquid tank is provided with and one or morely is communicated with outside openings, and described opening is by allowing gas permeation but the filtrator sealing that does not allow the hydrophobic nature perforated membrane of liquid or solid material permeance to make.
4. cartridge for chemical reaction according to claim 3, wherein said airtight cartridge for chemical reaction has: a plurality of chambers that are used for storing sample or reagent or treating fluid respectively; Described sample or reagent or treating fluid are transfused to reaction chamber wherein; And the flow passage that connects described chamber and described waste liquid tank,
A kind of device that is used for that described sample or reagent or treating fluid transferred to described reaction chamber and described waste liquid is transferred to described waste liquid tank is set simultaneously.
5. cartridge for chemical reaction according to claim 3, wherein said cartridge for chemical reaction are preparation rooms on laboratory or the chip on the box that is used for genetic diagnosis, the biological box that contains chip that is used for environment measurement or the chip that uses μ TAS.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100906027A CN100462710C (en) | 2004-11-09 | 2004-11-09 | Process for processing waste liquid in box and chemical reaction box using the same process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100906027A CN100462710C (en) | 2004-11-09 | 2004-11-09 | Process for processing waste liquid in box and chemical reaction box using the same process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1772632A CN1772632A (en) | 2006-05-17 |
| CN100462710C true CN100462710C (en) | 2009-02-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100906027A Expired - Fee Related CN100462710C (en) | 2004-11-09 | 2004-11-09 | Process for processing waste liquid in box and chemical reaction box using the same process |
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| Country | Link |
|---|---|
| CN (1) | CN100462710C (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111744562A (en) * | 2019-03-28 | 2020-10-09 | 天津大学 | Micro-fluidic chip for detecting micro-pollutants |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1986006488A1 (en) * | 1985-04-29 | 1986-11-06 | Hichem Diagnostics, Inc., Dba Bural Technologies | Diagnostic test kit |
| EP0381501A2 (en) * | 1989-02-03 | 1990-08-08 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
| CN1143917A (en) * | 1994-11-14 | 1997-02-26 | 宾夕法尼亚州大学信托人 | Mesoscale sample preparation device and system for determination and processing of analytes |
| US5863502A (en) * | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
| EP1201304A2 (en) * | 2000-10-25 | 2002-05-02 | MICROPARTS GESELLSCHAFT FÜR MIKROSTRUKTURTECHNIK mbH | Microstructured platform for examining a liquid |
| WO2003065039A1 (en) * | 2002-01-25 | 2003-08-07 | Matsushita Electric Industrial Co., Ltd. | Specimen analysis disk and specimen analysis device |
| WO2004010760A2 (en) * | 2002-07-26 | 2004-02-05 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
| DE10306018A1 (en) * | 2003-02-13 | 2004-09-09 | Siemens Ag | Instrument, for home blood tests, has a disinfecting unit to pass a disinfecting fluid through the analysis chip with its carrier and biosensor, to prevent disposal of contaminated chips with the waste and rubbish |
-
2004
- 2004-11-09 CN CNB2004100906027A patent/CN100462710C/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1986006488A1 (en) * | 1985-04-29 | 1986-11-06 | Hichem Diagnostics, Inc., Dba Bural Technologies | Diagnostic test kit |
| EP0381501A2 (en) * | 1989-02-03 | 1990-08-08 | Eastman Kodak Company | Containment cuvette for PCR and method of use |
| CN1143917A (en) * | 1994-11-14 | 1997-02-26 | 宾夕法尼亚州大学信托人 | Mesoscale sample preparation device and system for determination and processing of analytes |
| US5863502A (en) * | 1996-01-24 | 1999-01-26 | Sarnoff Corporation | Parallel reaction cassette and associated devices |
| EP1201304A2 (en) * | 2000-10-25 | 2002-05-02 | MICROPARTS GESELLSCHAFT FÜR MIKROSTRUKTURTECHNIK mbH | Microstructured platform for examining a liquid |
| WO2003065039A1 (en) * | 2002-01-25 | 2003-08-07 | Matsushita Electric Industrial Co., Ltd. | Specimen analysis disk and specimen analysis device |
| WO2004010760A2 (en) * | 2002-07-26 | 2004-02-05 | Applera Corporation | Microfluidic size-exclusion devices, systems, and methods |
| DE10306018A1 (en) * | 2003-02-13 | 2004-09-09 | Siemens Ag | Instrument, for home blood tests, has a disinfecting unit to pass a disinfecting fluid through the analysis chip with its carrier and biosensor, to prevent disposal of contaminated chips with the waste and rubbish |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1772632A (en) | 2006-05-17 |
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