CN100434507C - Pleurotus ostreatus KZH-2 and its preparing process - Google Patents
Pleurotus ostreatus KZH-2 and its preparing process Download PDFInfo
- Publication number
- CN100434507C CN100434507C CNB2006100108469A CN200610010846A CN100434507C CN 100434507 C CN100434507 C CN 100434507C CN B2006100108469 A CNB2006100108469 A CN B2006100108469A CN 200610010846 A CN200610010846 A CN 200610010846A CN 100434507 C CN100434507 C CN 100434507C
- Authority
- CN
- China
- Prior art keywords
- test tube
- flat mushroom
- strain
- kzh
- bacterial strain
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a pleurotus ostreatus bacterial strain KZH-2 and a preparing method thereof, which belong to the technical field of microorganisms. The pleurotus osteatus bacterial strain KZH-1 of the present invention is preserved in the common microorganism center of the China committee for culture collection of microorganisms on January 25th, 2006, and the preserving number is CGMCC NO. 1601. When the solid excellent strain prepared by the bacterial strain is used for the cultivation and the production of pleurotus ostreatus, obvious social and economic benefits and wide applying prospects can be obtained.
Description
Technical field
The invention belongs to microbial technology field, be specifically related to flat mushroom bacterial strain KZH-2 and preparation method.
Background technology
Flat mushroom Pleurotus ostreatus is the edible fungus variety of present cultivation amount maximum, existing flat mushroom kind is along with the prolongation of cultivation and duration of service, individual cell age is increasing, the metabolism function lowers gradually, lose anti-adversity ability, gradually lose original good character, the commodity performance reduces, so that lose its use value.Bacterial classification heredity changes; Bacterial classification is parasitic by virus; Culture condition is not suitable for, and can not satisfy its life requirement, makes it lose capacity of self-regulation, and losing factor such as normal physiological function is to cause the reason of spawn degeneration, is the gradual change result of quantitative change to qualitative change, also is a kind of morbid state and old and feeble general performance.At present mainly cultivate new bacterial strain and former bacterial strain proterties is carried out rejuvenation, guarantee the good characteristic of bacterial classification by methods such as natural seed selection, cross-breeding, selection by mutation, bacterial classification detoxifications.The nature seed selection does not need special equipment, but will carry out a large amount of simultaneous tests by collecting the bacterial strain of growing under a large amount of different conditions, and the time is long, and workload is big; Cross-breeding, selection by mutation, bacterial classification detoxification then need specific installation to finish, and complex process is subjected to multiple factor affecting, unstable result.
Summary of the invention
The present invention has overcome the deficiency of aforesaid method, utilizes wild flat mushroom resource, by sporophore separation, purifying, fruiting experiment screening, cultivate that growth is strong, adaptability strong, the good strain excellent of commodity performance tool.
The fungi that the present invention adopts is flat mushroom Pleurotus ostreatus KZH-2; Depositary institution: China Committee for Culture Collection of Microorganisms common micro-organisms center; Address: China. Beijing. the Zhong Guan-cun; Preservation date: on 01 25th, 2006; The numbering CGMCC NO.1601 that preservation is registered on the books.
This mushroom carpophore is medium to bigger, and bacteria cover diameter 5-18 centimetre, canescence, flat semisphere, open and flat back is fan-shaped, near the recessed hairiness of shank; Bacterial context white is thick slightly; Lamella white, close slightly; Stem adnation, weak point, interior real, white, long 1-3 centimetre, thick 1-2 centimetre, base portion tool fine hair; Spore print white, spore are closely cylindrical, and is smooth colourless.22-26 ℃ of mycelium culture temperature, 5-28 ℃ of fruiting temperature.This bacterial strain adaptability is strong, high yield and high quality, and stable performance is fit to multiple culture material cultivation.
Technical scheme of the present invention:
1. gather wild mushroom carpophore;
2. separate tissue;
3. purifying bacterial strain and identification of strains;
4. fruiting experiment (biological characteristics relatively reach the production traits relatively);
5. determine strain excellent;
6. bacterial classification production and bacterial classification are used;
Through repeated screening, obtain the flat mushroom bacterial strain KZH-2 of output height, strong stress resistance.
Fungi Pleurotus ostreatusKZH-2 cultural method of the present invention:
Strain separating purifying substratum: (PDA substratum)
Potato 200 grams, glucose 20 grams, agar 20 grams are converted 1000 milliliters in water.
Flat mushroom strains separation and authentication method:
1, the mushroom carpophore tissue block is inoculated on the above-mentioned test tube nutrient agar inclined-plane, cultivated 5-7 days down in 25 ℃, to test tube observed and recorded every day of separation and Culture, the test tube that rejecting has in time been polluted, pick out bacterial strain pollution-free, that grow fine, obtain flat mushroom and separate the test tube kind.
2, will separate test tube kind inoculated by hypha block and to the dull and stereotyped culture dish of above-mentioned nutrient agar, carry out the bacterial classification purifying, get most advanced and sophisticated inoculated by hypha block after 2 days on medium slant, cultivate 7 days down, obtain flat mushroom purifying test tube kind in 25 ℃.
3, with flat mushroom purifying test tube kind, make the secondary kind, produce cultivar, carry out fruiting experiment, obtain sporophore, determine that separating the pure strain that obtains is flat mushroom strain.
The preparation method of flat mushroom strain of the present invention is divided into: two kinds of solid spawn and liquid spawns.
The solid spawn preparation method:
The solid culture based formulas is: 1. wheat or buckwheat grain 83%, weed tree sawdust 15%, terra alba or lime carbonate 2%.
2. weed tree sawdust 78%, wheat bran 20%, sucrose 1%, gypsum 1%, water content 60%, PH nature.
3. cotton seed hulls 35%, wood chip 45%, wheat bran 18%, sucrose 1%, gypsum 1%, water content 60%, PH nature.
1, the mycelium with flat mushroom is inoculated on the test tube nutrient agar inclined-plane, and culture medium prescription is the PDA substratum, cultivates 7-8 days down in 25 ℃, obtains test tube strains.
2, adopt aforementioned solid culture based formulas.After culture material mixed, after adding water and mixing evenly, in the jar of the 750ml that packs into, compress and seal the back,, insert test tube strains, cultivated 25-30 days in 25 ℃, cover with up to mycelia in the sterilisable chamber cooling 121 ℃ of sterilizations 120 minutes.
The liquid spawn preparation method:
The liquid culture based formulas is: potato 20%, glucose 2%, peptone 0.2%, potassium primary phosphate 0.05%, sal epsom 0.05%, remainder is a water, the PH nature.
1, the mycelium with flat mushroom is inoculated on the test tube nutrient agar inclined-plane, and culture medium prescription is the PDA substratum, cultivates 7-8 days down in 25 ℃, obtains test tube strains.
2, nutrient solution is packed into (every bottled 100ml) in the 500ml triangular flask, culture medium prescription is the aforementioned liquids culture medium prescription, seals with tampon and kraft paper, and sterilization is 30 minutes under 0.15 MPa pressure, takes out, and is chilled to inoculation in 30 o'clock.
3, the test tube kind is inoculated in 500ml triangular flask (the every bottled 100ml) liquid nutrient medium, cultivates in 25 ℃ of following shaking tables, rotating speed is 150r/min, incubation time 5-7 days.
4, will shake bottle bacterial classification inoculation in the fermentor tank of the automatic fermentative production line of 25L, air flow is 1: 0.8v/ (vmin); Mixing speed is 150r/min; Inoculum size is 5%; Tank pressure: 0.04Mpa; PH is 6.0; Temperature is 25 ℃; Aerated culture 72 hours obtains the flat mushroom liquid spawn.
Embodiment
Embodiment one:
1, wild mushroom carpophore tissue block is inoculated on the above-mentioned strain separating purifying medium slant, cultivated 7 days down in 25 ℃, to test tube observed and recorded every day of separation and Culture, the test tube that rejecting has in time been polluted, pick out bacterial strain pollution-free, that grow fine, obtain flat mushroom and separate the test tube kind.
2, will separate test tube kind inoculated by hypha block and to the dull and stereotyped culture dish of above-mentioned nutrient agar, carry out the bacterial classification purifying, get most advanced and sophisticated inoculated by hypha block after 2 days on medium slant, cultivate 7 days down, obtain flat mushroom purifying test tube kind in 25 ℃.
3, with flat mushroom purifying test tube kind, make the secondary kind, produce cultivar, carry out fruiting experiment, obtain sporophore, determine that separating the pure strain that obtains is flat mushroom strain.
4, the mycelium with Pleurotus ostreatusKZH-2 is inoculated on the test tube nutrient agar inclined-plane, and culture medium prescription is the PDA substratum, cultivates 7 days down, obtains the test tube kind for 25 ℃.
5, adopt solid culture based formulas wheat or buckwheat grain 83%, weed tree sawdust 15%, terra alba or lime carbonate 2%.Wheat or buckwheat grain clear water wash clean, soaked about 15 hours earlier, leach with 2% liming; boiled 15 minutes, and poured out drainage, add weed tree sawdust; terra alba or lime carbonate are mixed thoroughly, and 750 milliliters the special bacteria bottle of packing into seals; sterilized 120 minutes down at 121 ℃; in the sterilisable chamber cooling, insert test tube strains, cultivated 30 days in 25 ℃; cover with up to mycelia, obtain bacterial classification.
Embodiment two:
1, wild mushroom carpophore tissue block is inoculated on the above-mentioned strain separating purifying medium slant, cultivated 7 days down in 25 ℃, to test tube observed and recorded every day of separation and Culture, the test tube that rejecting has in time been polluted, pick out bacterial strain pollution-free, that grow fine, obtain flat mushroom and separate the test tube kind.
2, will separate test tube kind inoculated by hypha block and to the dull and stereotyped culture dish of above-mentioned nutrient agar, carry out the bacterial classification purifying, get most advanced and sophisticated inoculated by hypha block after 2 days on medium slant, cultivate 7 days down, obtain flat mushroom purifying test tube kind in 25 ℃.
3, with flat mushroom purifying test tube kind, make the secondary kind, produce cultivar, carry out fruiting experiment, obtain sporophore, determine that separating the pure strain that obtains is flat mushroom strain.
4, the mycelium with Pleurotus ostreatusKZH-2 is inoculated on the test tube nutrient agar inclined-plane, and culture medium prescription is the PDA substratum, cultivates 7 days down, obtains the test tube kind for 25 ℃.
5, adopt aforementioned solid culture based formulas weed tree sawdust 78%, wheat bran 20%, sucrose 1%, gypsum 1%, water content 60%, PH nature.Culture material is mixed, after adding water and mixing evenly, in the jar of the 750ml that packs into, compress and seal the back,, insert test tube strains, cultivated 30 days in 25 ℃, cover with, obtain the flat mushroom solid spawn up to mycelia in the sterilisable chamber cooling 121 ℃ of sterilizations 120 minutes.
Embodiment three:
1, wild mushroom carpophore tissue block is inoculated on the above-mentioned strain separating purifying medium slant, cultivated 7 days down in 25 ℃, to test tube observed and recorded every day of separation and Culture, the test tube that rejecting has in time been polluted, pick out bacterial strain pollution-free, that grow fine, obtain flat mushroom and separate the test tube kind.
2, will separate test tube kind inoculated by hypha block and to the dull and stereotyped culture dish of above-mentioned nutrient agar, carry out the bacterial classification purifying, get most advanced and sophisticated inoculated by hypha block after 2 days on medium slant, cultivate 7 days down, obtain flat mushroom purifying test tube kind in 25 ℃.
3, with flat mushroom purifying test tube kind, make the secondary kind, produce cultivar, carry out fruiting experiment, obtain sporophore, determine that separating the pure strain that obtains is flat mushroom strain.
4, the mycelium with Pleurotus ostreatusKZH-2 is inoculated on the test tube nutrient agar inclined-plane, and culture medium prescription is the PDA substratum, cultivates 7 days down, obtains the test tube kind for 25 ℃.
5, adopt aforementioned solid culture based formulas cotton seed hulls 35%, wood chip 45%, wheat bran 18%, sucrose 1%, gypsum 1%, water content 60%, PH nature.Culture material is mixed, after adding water and mixing evenly, in the jar of the 750ml that packs into, compress and seal the back,, insert test tube strains, cultivated 30 days in 25 ℃, cover with, obtain the flat mushroom solid spawn up to mycelia in the sterilisable chamber cooling 121 ℃ of sterilizations 120 minutes.
Embodiment four:
1, wild mushroom carpophore tissue block is inoculated on the above-mentioned strain separating purifying medium slant, cultivated 7 days down in 25 ℃, to test tube observed and recorded every day of separation and Culture, the test tube that rejecting has in time been polluted, pick out bacterial strain pollution-free, that grow fine, obtain flat mushroom and separate the test tube kind.
2, will separate test tube kind inoculated by hypha block and to the dull and stereotyped culture dish of above-mentioned nutrient agar, carry out the bacterial classification purifying, get most advanced and sophisticated inoculated by hypha block after 2 days on medium slant, cultivate 7 days down, obtain flat mushroom purifying test tube kind in 25 ℃.
3, with flat mushroom purifying test tube kind, make the secondary kind, produce cultivar, carry out fruiting experiment, obtain sporophore, determine that separating the pure strain that obtains is flat mushroom strain.
4, the mycelium with Pleurotus ostreatusKZH-2 is inoculated on the test tube nutrient agar inclined-plane, and culture medium prescription is the PDA substratum, cultivates 7 days down, obtains the test tube kind for 25 ℃.
5, the test tube kind is inoculated in the 500ml triangular flask in (every bottled 100ml) liquid nutrient medium, culture medium prescription is: potato 20%, glucose 2%, peptone 0.2%, potassium primary phosphate 0.05%, sal epsom 0.05%, remainder is a water, the PH nature.Cultivate in 25 ℃ of following shaking tables, rotating speed is 150rpm, and incubation time 7 days obtains the level liquid bacterial classification.
In the fermentor tank of the automatic fermentative production line of 25L, air flow is 1 with the level liquid bacterial classification inoculation: 0.8v/ (vmin); Mixing speed is 150r/min; Inoculum size is 5%; Tank pressure: 0.04Mpa; PH is 6.0; Temperature is 25 ℃; Aerated culture 72 hours obtains the flat mushroom liquid spawn.
Claims (1)
1, a kind of flat mushroom bacterial strain KZH-2 is characterized in that described bacterial strain is flat mushroom (Pleurotus ostreatus) bacterial strain KZH-2, and the preserving number of this bacterial strain is CGMCC NO.1601.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100108469A CN100434507C (en) | 2006-04-25 | 2006-04-25 | Pleurotus ostreatus KZH-2 and its preparing process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100108469A CN100434507C (en) | 2006-04-25 | 2006-04-25 | Pleurotus ostreatus KZH-2 and its preparing process |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1844351A CN1844351A (en) | 2006-10-11 |
| CN100434507C true CN100434507C (en) | 2008-11-19 |
Family
ID=37063321
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2006100108469A Expired - Fee Related CN100434507C (en) | 2006-04-25 | 2006-04-25 | Pleurotus ostreatus KZH-2 and its preparing process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100434507C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107641600A (en) * | 2017-10-10 | 2018-01-30 | 河北省科学院生物研究所 | Suitable for the flat mushroom JK02 bacterial strains and its cultural method of low temperature fruiting and application |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102986536B (en) * | 2012-12-02 | 2016-12-21 | 中华全国供销合作总社昆明食用菌研究所 | A kind of flammulina velutipes strain and preparation method |
| CN103858668B (en) * | 2013-11-29 | 2016-02-17 | 中华全国供销合作总社昆明食用菌研究所 | A kind of wool mushroom mycopremna and process for preparing strain thereof |
| CN105087388A (en) * | 2015-07-16 | 2015-11-25 | 漳州市农业科学研究所 | Preparation method of original strain of pleurotus cornucopiae |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1164316A (en) * | 1996-05-08 | 1997-11-12 | 崔善金 | High quality and high yield agaricus tabularis cultivation seed culture new technology |
| WO2002068611A1 (en) * | 2001-02-27 | 2002-09-06 | Environment & Energy Technology | Method and system of disposing food wastes |
| JP2003158920A (en) * | 2001-11-28 | 2003-06-03 | Marine Bio Kk | Vanadium-containing mushroom and functional food using the same, and method for producing the mushroom and the functional food |
-
2006
- 2006-04-25 CN CNB2006100108469A patent/CN100434507C/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1164316A (en) * | 1996-05-08 | 1997-11-12 | 崔善金 | High quality and high yield agaricus tabularis cultivation seed culture new technology |
| WO2002068611A1 (en) * | 2001-02-27 | 2002-09-06 | Environment & Energy Technology | Method and system of disposing food wastes |
| JP2003158920A (en) * | 2001-11-28 | 2003-06-03 | Marine Bio Kk | Vanadium-containing mushroom and functional food using the same, and method for producing the mushroom and the functional food |
Non-Patent Citations (4)
| Title |
|---|
| 平菇优良菌种筛选及其营养成分研究. 王钊，梁瑞璋.中国食用菌，第18卷第2期. 1999 |
| 平菇优良菌种筛选及其营养成分研究. 王钊，梁瑞璋.中国食用菌，第18卷第2期. 1999 * |
| 白平菇优良菌株选育研究初报. 黄清荣等.中国食用菌，第23卷第2期. 2004 |
| 白平菇优良菌株选育研究初报. 黄清荣等.中国食用菌，第23卷第2期. 2004 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107641600A (en) * | 2017-10-10 | 2018-01-30 | 河北省科学院生物研究所 | Suitable for the flat mushroom JK02 bacterial strains and its cultural method of low temperature fruiting and application |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1844351A (en) | 2006-10-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100434506C (en) | Screening for strain of steepletop hickory chick and process for preparing strain thereof | |
| CN104560827B (en) | A kind of biocontrol actinomycetes bacterial strain for preventing and treating tobacco bacterial wilt and its application | |
| CN109757307A (en) | Xianggu mushroom strain and its industrial planting method suitable for factory culture | |
| CN100340654C (en) | Screening for strain of balck vein hickory chick and process for preparing strain thereof | |
| CN101558766B (en) | Trichoderma solid granules for preventing and controlling tobacco soil-borne fungus diseases and preparation method thereof | |
| CN103305430B (en) | Laccase generation cerrena and application thereof | |
| Rossi et al. | Inoculant production of ectomycorrhizal fungi by solid and submerged fermentations | |
| CN101790937B (en) | Screening and culture preparing method of Russula.alutacea strain | |
| CN104988082A (en) | Debaryomyces hansenii strain and applications thereof | |
| CN101933439A (en) | Method for improving phellinus igniarius hypha amount of submerged culture by utilizing plant oil | |
| CN100434507C (en) | Pleurotus ostreatus KZH-2 and its preparing process | |
| CN101622939B (en) | Inonotus obliquus deep culture method | |
| CN102986536B (en) | A kind of flammulina velutipes strain and preparation method | |
| CN109234176A (en) | Cordyceps sinensis mother culture media and preparation method thereof | |
| CN100462431C (en) | Pleurotus ostreatus KZH-1 and its preparing process | |
| CN116042489A (en) | Lotus root endophytic bacillus beijerinus strain LS8 and application thereof | |
| CN102986537A (en) | Tricholoma lobayense strain KJH-3 and preparation method thereof | |
| CN103004465A (en) | Coprinus comatus strain and preparation method | |
| CN103305481B (en) | Method for producing laccase by fermenting cerrena unicolor | |
| CN103525710B (en) | Penicillium oxalicum BAM-1 and separation purification method thereof and application | |
| CN109136100A (en) | One plant of Tabin aspergillus bacterial strain and the application on fermentation green brick tea | |
| CN102492628B (en) | Fungus for promoting symbiotic germination of cypripedium macranthum seeds and application thereof | |
| CN106085880B (en) | A kind of separation method and used medium of smut | |
| CN107641602A (en) | One plant of candida utili and its fermentation lay eggs it is white in application | |
| CN103858668B (en) | A kind of wool mushroom mycopremna and process for preparing strain thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081119 |