CN100415873C - Bacteria strain (X-7 strain) for culturing bait eelworm and its eelworm culture method - Google Patents
Bacteria strain (X-7 strain) for culturing bait eelworm and its eelworm culture method Download PDFInfo
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- CN100415873C CN100415873C CNB2006101228010A CN200610122801A CN100415873C CN 100415873 C CN100415873 C CN 100415873C CN B2006101228010 A CNB2006101228010 A CN B2006101228010A CN 200610122801 A CN200610122801 A CN 200610122801A CN 100415873 C CN100415873 C CN 100415873C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
The invention discloses a bacterium strain (X-7 system) to culture bait nematolog and nematolog culturing method, which comprises the following steps: culturing Xenorhabdus sp. X-7 CCTCC M 206090 on the culture medium with peptone water under 25-28 deg. c to obtain the bacterial liquid; adding nematolog culture medium; switching in Panagrellus redivivus; culturing under 25-28 deg. c for 6-15h; obtaining the product as bait in the aquatic product culturing industry.
Description
Technical field
The present invention relates to a kind of be used to the cultivate bacterium of bait nematode and the cultural method of bait nematode Panagrellus redivivus thereof.
Background technology
The development of fishery products seedling cultures has been moved in the breed torrid zone of worldwide aquaculture, particularly prawn, the demand of also having aggravated biological feed, the especially demand of halogen worm.The halogen worm is generally picked up from the high salinity waters, and its intensive culture does not form scale as yet.At first, the Great Salt lake of Utah, USA is the place that abounds with the halogen worm, and its artemia cysts output accounts for more than 80% of world wide production always for many years, the supply of left and right sides world market.But since the nineties, because the excessive receipts of artemia resource are adopted and the change of salinity of lake water, the halogen worm output in salt lake sharply descends, and has caused the expensive of halogen worm.Central Asia also supplies the halogen worm, but because the instability of the halogen worm quality of producing, the demand in market does not satisfy yet.For needs and the conservation of nature artemia resource that satisfies market, be necessary to seek novel biological feed to substitute or the alternative present widely used halogen worm bait of part.
At present, free-living nematode Panagrellus redivivus (a kind of Rhabditida saprophitic nematode, ovoviviparity; Chinese name is undetermined) as biological feed (Schlechtriem et al. (2004) Aquaculture Research 35, the 547-551 of fishes and shrimps seedling; Briedenbach et al. (1989) Journal of World Aquaculture Society 20,61-71; Hofsten et al (1983) Journal of Fish biology 23,419-428.).It is little that this class nematode has a volume as biological feed, can survive in seawater more than 72 hours, and individuality can excessively not increase and make juvenile prawn be difficult to digestion, is of high nutritive value, and generation time is short, and reproductivity is strong, the simple advantage of cultural method.Therefore be the suitable senior living organism bait of aquatic product sprouts such as fish, shrimp crab.The cultivation of P.redivivus nematode is generally adopted in substratum and to be added that intestinal bacteria or yeast realize.Intestinal bacteria or saccharomycetic adding mainly are directly to provide nematode to breed needed nutrition, perhaps transform substratum and provide nutrition for nematode in reproductive process.But, on commercial applications,, therefore, be difficult to use in the large scale culturing of biological feed because intestinal bacteria are human pathogenic bacterias; Though yeast is safe as biological feed,, saccharomycetic cultivation, particularly liquid culture are convenient not as bacterium.Therefore, be necessary to select new bacterial strain to cultivate this biological feed nematode.
Summary of the invention
The objective of the invention is to propose a kind of bacterial isolates that can be used to cultivate this biological feed nematode of Panagrellus redivivus.
Another object of the present invention provides the novel method of a kind of Panagrellus of cultivation redivivus biological feed nematode.
The bacterial isolates that is used to cultivate Panagrellus redivivus biological feed nematode proposed by the invention, Xenorhabdus sp.X-7 strain bacterium for the Xenorhabdus genus, this bacterial strain is isolating in the entomopathogenic nematode body of obligatory parasitism insect, Gram-negative belongs to enterobacteriaceae (Enterobacteriaceae).The catalase feminine gender; Nitrate can not be degraded to nitrite; Nascent type thalline has whole body flagellum; Do not produce bioluminescence.On the LB solid culture, the bacterium colony of Xenorhabdus sp.X-7 bacterial strain presents khaki color.
This bacterial isolates has been preserved in Chinese typical culture collection center (CCTCC), and its deposit number is CCTCC NO:M 206090.
The cultural method of the biological feed nematode that the present invention proposes is, adopt strain X enorhabdus sp.X-7 CCTCC M206090 as producing bacterium, its culturing step comprises: with bacterial isolates Xenorhabdus sp.X-7 CCTCC M 206090 on peptone water medium, under 25-28 ℃, cultivate and obtain bacterium liquid, then it is added nematode culture medium, insert aseptic Panagrellus redivivus nematode again, place 25-28 ℃ to cultivate 6 to 15 days down, can gather in the crops the nematode of being cultivated.
In the cultural method of described biological feed nematode, described peptone water medium recommends to adopt the peptone water medium that contains 1% (mass percent) peptone and 0.5% (mass percent) sodium-chlor.
The set of dispense of the nematode culture medium of recommending than (by mass percentage) is: yeast extract paste 1%, flour 10%, analysis for soybean powder 5%, lard 4%, water 65%, sponge 15%.
Entomopathogenic nematode is present novel biological pesticide, because its security and validity, many industrialized countries exempt registration and can discharge.Xenorhabdus sp.X-7 CCTCC M 206090 utilizes isolating Xenorhabdus genus symbiotic bacterium in this class entomopathogenic nematode body, utilize it to cultivate the P.redivivus nematode not only to environment and person poultry safety, and cultivate the particularly cultivation of large fermentation tank easily.The method that the present invention describes can promote the industrialization of P.redivivus nematode to cultivate, for culture fishery provides good biological feed.
Embodiment
Following experiment and operational instances are further to explanation of the present invention, should not be used as limitation of the present invention.
1. strain X enorhabdus sp.X-7 CCTCC M 206090 is being contained 1% (mass percent) peptone, on the peptone water medium of 0.5% (mass percent) sodium-chlor, cultivating the bacterium liquid that obtains the Xenorhabdus genus down in 26 ℃;
2. nematode culture medium was sterilized 40 minutes down at 121 ℃, the set of dispense of nematode culture medium than (by mass percentage) is: yeast extract paste 1%, flour 10%, analysis for soybean powder 5%, lard 4%, water 65%, sponge 15%;
3. 100 grams are added in the triangular flask of 500ml through the nematode culture medium of sterilization, the bacterium liquid that adds the Xenorhabdus genus of being cultivated again, insert aseptic P.redivivus nematode then, place 25-28 ℃ to cultivate down, the nematode that results are cultivated after 6 to 15 days can be gathered in the crops the nematode in the culture.
Do above-mentioned during with the experiment of Xenorhabdus sp.X-7 CCTCC M 206090 strain culturing Panagrellus redivivus biological feed nematodes, the contriver has made to insert the Invscl of intestinal bacteria (Escherichia coli) EPI100 strain and saccharomyces cerevisiae in same substratum, the simultaneous test of Gim2.8 and R12 strain, these cultures place 25-28 ℃ to cultivate down equally.Calculated the output of nematode in the culture in 6 to 15 days.
The result shows: insert respectively in the culturing bottle of Invscl, the Gim2.8 of Xenorhabdus sp.X-7 CCTCC M 206090 bacterial strains, intestinal bacteria EPI100 strain, saccharomyces cerevisiae and R12 strain bacterium liquid, every bottle of output that contains 100 gram substratum is respectively 3135,2755,3470, article 3000 and 1,140 ten thousand, P.redivivus nematode.As seen, the bacterial isolates of Xenorhabdus sp.X-7 CCTCCM 206090 can be supported the breeding of P.redivivus nematode, and obtains higher output.
Claims (4)
1. a bacterial isolates that is used to cultivate the biological feed nematode is characterized in that this bacterial isolates is Xenorhabdus (Xenorhabdus sp.) the X-7CCTCC M 206090 that is preserved in Chinese typical culture collection center.
2. the cultural method of a biological feed nematode, it is characterized in that: adopt Xenorhabdus (Xenorhabdus sp.) X-7CCTCC M 206090 as producing bacterium, its culturing step comprises: with Xenorhabdus (Xenorhabdus sp.) X-7CCTCCM 206090 on peptone water medium, under 25-28 ℃, cultivate and obtain bacterium liquid, then it is added nematode culture medium, insert aseptic Panagrellus redivivus nematode again, place 25-28 ℃ to cultivate 6 to 15 days down, can gather in the crops the nematode of being cultivated.
3. the cultural method of biological feed nematode according to claim 1 is characterized in that it is that 1% peptone and mass percent are 0.5% sodium-chlor that described peptone water medium contains mass percent.
4. the cultural method of biological feed nematode according to claim 1 is characterized in that the set of dispense of described nematode culture medium than by mass percentage is: yeast extract paste 1%, flour 10%, analysis for soybean powder 5%, lard 4%, water 65%, sponge 15%.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2006101228010A CN100415873C (en) | 2006-10-17 | 2006-10-17 | Bacteria strain (X-7 strain) for culturing bait eelworm and its eelworm culture method |
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| CNB2006101228010A CN100415873C (en) | 2006-10-17 | 2006-10-17 | Bacteria strain (X-7 strain) for culturing bait eelworm and its eelworm culture method |
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| CN1995329A CN1995329A (en) | 2007-07-11 |
| CN100415873C true CN100415873C (en) | 2008-09-03 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101485300B (en) * | 2008-01-18 | 2011-05-11 | 中国科学院沈阳应用生态研究所 | Method for cultivating Acrobeloides nanus |
| CN104737986A (en) * | 2013-12-30 | 2015-07-01 | 南开大学 | Optimized solid medium for producing entomopathogenic nematodes |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300817A (en) * | 1999-12-17 | 2001-06-27 | 广东省昆虫研究所 | Liquid culture method for pathogenic nematode of insect |
| WO2003001909A2 (en) * | 2001-06-29 | 2003-01-09 | Fish Biotech Ltd. | A process for storing enriched nematodes |
| CN1724649A (en) * | 2005-06-16 | 2006-01-25 | 西北农林科技大学无公害农药研究服务中心 | Entomopathogenic nematode symbiotic bacteria fermentation process and application of fermentation liquor thereof |
| CN1724651A (en) * | 2005-06-16 | 2006-01-25 | 西北农林科技大学无公害农药研究服务中心 | Segmented oxygen supply fermentation process of entomopathogenic nematode symbiotic bacteria and use of fermentation product thereof |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1300817A (en) * | 1999-12-17 | 2001-06-27 | 广东省昆虫研究所 | Liquid culture method for pathogenic nematode of insect |
| WO2003001909A2 (en) * | 2001-06-29 | 2003-01-09 | Fish Biotech Ltd. | A process for storing enriched nematodes |
| CN1724649A (en) * | 2005-06-16 | 2006-01-25 | 西北农林科技大学无公害农药研究服务中心 | Entomopathogenic nematode symbiotic bacteria fermentation process and application of fermentation liquor thereof |
| CN1724651A (en) * | 2005-06-16 | 2006-01-25 | 西北农林科技大学无公害农药研究服务中心 | Segmented oxygen supply fermentation process of entomopathogenic nematode symbiotic bacteria and use of fermentation product thereof |
Non-Patent Citations (2)
| Title |
|---|
| 昆虫病原线虫与其共生细菌共生关系的研究进展. 夏颖伟等人.昆虫知识，第43卷第3期. 2006 |
| 昆虫病原线虫与其共生细菌共生关系的研究进展. 夏颖伟等人.昆虫知识，第43卷第3期. 2006 * |
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