CN100408110C - Production method of absorbable suture of mammal nerve fiber - Google Patents
Production method of absorbable suture of mammal nerve fiber Download PDFInfo
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- CN100408110C CN100408110C CNB2006100791272A CN200610079127A CN100408110C CN 100408110 C CN100408110 C CN 100408110C CN B2006100791272 A CNB2006100791272 A CN B2006100791272A CN 200610079127 A CN200610079127 A CN 200610079127A CN 100408110 C CN100408110 C CN 100408110C
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Abstract
The present invention relates to a method for preparing and manufacturing absorbable suture threads from nerve fiber of mammals, which has the steps: washing nerve fiber bundles of the tail parts of adult vertebra mammal rodents by clear water to remove the gore on the surfaces, and then cleaning the nerve fiber bundles in hank's; degreasing and dewatering the nerve fiber bundles by alcohol of different concentration, and then soaking the nerve fiber bundles in pepsin solution and endonuclease solution; rinsing the soaked nerve fiber bundles in 5% papain. In the method of the present invention, the nerve fiber bundles of tails are disengaged naturally to form hundreds of independently integral fiber threads, so that the present invention greatly enhances the number of prepared suture threads(the extraction rate is close to 100%), prevents from wasting raw material resources, manufactures the nerve fiber bundles to have smooth surfaces without burr, keeps the original characteristics of favorable elasticity and high flexibility of the nerve fiber of the mammals, eliminates the original harmful tissue in the nerve fiber of the mammals, thoroughly eradicates the rejection phenomenon between a human body and suture threads after operative suture, and further lightens the pain of patients after operation.
Description
Technical field
The present invention relates in a kind of medical supplies suture produce manufacture method, particularly relate to a kind of production method of absorbable suture of mammal nerve fiber.
Background technology
In the prior art when hospital handles skin injury and operate on operation, carry out multi-level stitching to skin and subcutaneous tissue and organ, for the stitching of skin surface, be to adopt the higher nylon suture of intensity mostly, after the affected part recovers from injury, can take out by taking out stitches; And for the stitching of internal layer, stitching thread can not be taken out again, the stitching thread that has only employing to be absorbed voluntarily by human body, in the prior art, absorbable stitching thread mainly just is to use special-purpose catgut, and special-purpose catgut has certain rejection phenomenon to human body, often causes the subcutaneous lump that occurs in affected part, brings extra painful and infringement to the patient; Along with progress of science and technology, it is found that the laboratory animal use standard of vertebra mammals rodent near human physical signs, particularly nutria adopts growth and subsides meeting property and the no lymphocyte that its glue unit cultivates as cyton, can not produce the wound rejection phenomenon, find its collagen fiber moderate length simultaneously, the thickness difference, the operation stitching that flexible and traction force meets internal organs uses.
At present, in the prior art, produce this stitching thread, be to adopt peel manually mostly from the method that extracts, this mode causes its coccygeal nerve collagen fiber surface to be subjected to mechanicalness through regular meeting and damages, and its smooth in appearance is destroyed, and former cross sectional shape is compelled to drawing-down, destroy intrinsic fibre structure, influence its result of use; Greatly reduce the product line rate of each afterbody simultaneously, approximately can only output 15%, remainder then all slatterns, and so then improved cost of material greatly, and staff's working environment is relatively poor, the labor intensity height, the labor productive rate is low.
Summary of the invention
The purpose of this invention is to provide a kind of production method of absorbable suture of mammal nerve fiber, by the technical scheme of enzymolysis separation mammalian nervous fiber, to remedy the deficiencies in the prior art.
Production method of absorbable suture of mammal nerve fiber of the present invention is to carry out according to the following steps:
(1) Adult Mammals is put to death, remove the peel after cutting away its afterbody, the nerve fibre bundle that separates its afterbody is removed behind the surperficial blood stains standby with flushing with clean water.
(2) standby nerve fibre bundle being put into Hank ' s liquid cleans.
(3) be placed on and carry out in 95% alcohol liquid soaking in 72 hours, carry out ungrease treatment.
(4) be placed on and carry out in 90% alcohol liquid soaking in 24 hours, carry out processed.
(5) be placed on then in the pepsin solution of 3%--7% and soaked 24 hours, continue immersion 24 hours after changing identical new liquid.
(6) be placed in the restriction endonuclease solution of 2%--8% and soaked 36 hours, to resolve the outer fascia between the nerve fibre bundle.
(7) put into 5% papain and carry out rinsing.
(8) do the wash the cotton-shaped parsing foreign material of removal with cell pyrolysis liquid.
(9) change in the alcohol liquid of 6% calcium hypochlorite and 40% sodium chloride and 54% and carried out rinsing 48 hours.
(10) changing to the further infiltration of 70% ethanol and 28% hydrogen peroxide bleachinges and dyeing and Preservation Treatment.
(11) be positioned in the mixed solution of 70% ethanol and 25% glycerol and 5% glacial acetic acid, long-period freshness preserving is deposited under 4 ℃ of temperature, also can dry after radiation sterilization is handled, and carries out the long-time preservation in drying back.
As optimization, the preferred plan of described pepsin solution is that concentration is 6.4%; Described restriction endonuclease is 6%.
After the nerve fibre bundle that the present invention gets afterbody is removed surperficial blood stains with flushing with clean water, be placed among the hank ' s and clean, can protect its cell and nerve, protect its activity, also can play degreasing simultaneously; Through the defat and the dehydration of variable concentrations ethanol, be placed in the pepsin solution and soak again, to resolve muscle, lax flesh is opened, and places and soaks in the restriction endonuclease solution to resolve the outer fascia between the nerve fibre bundle; Put into 5% papain and carry out rinsing, papain enters the local nerve fiber, nerve fiber is resolved disperses, by technical matters method of the present invention, realize that enzymolysis separates the technical scheme of Mus coccygeal nerve collagen fiber, make the tail nerve fibre bundle realize that nature breaks away from, and becomes the single of up to a hundred independent completions.
Production method of absorbable suture of mammal nerve fiber beneficial effect of the present invention is, the process for making of producing by the technical program, 1, not only improved greatly and produced this stitching thread quantity, made its extraction ratio approach 100%, thereby stopped the waste of raw material resources; 2, produce the stitching thread produced on outward appearance, smooth surface does not have burr, and the good springiness that has kept original coccygeal nerve fiber, the characteristics that pliability is strong, 3, dispose unfavorable composition and tissue in the original coccygeal nerve fiber, the stitching thread that makes this method produce to produce, be more prone to be absorbed by human body, accelerate and improved the wound healing ability of human body, 4, by experiment as can be known, the present invention produces the stitching thread of producing, eliminate the harmful tissue in the original coccygeal nerve fiber, thoroughly eradicated the rejection phenomenon between the human body and stitching thread behind the operation stitching, further alleviated the misery behind the corrective surgery.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
The embodiment of production method of absorbable suture of mammal nerve fiber of the present invention carries out according to the following steps:
(1) the vertebra mammals rodent that will grow up is put to death, and removes the peel after cutting away its afterbody, and the nerve fibre bundle that separates its afterbody is removed behind the surperficial blood stains standby with flushing with clean water.
(2) standby nerve fibre bundle being put into Hank ' s liquid cleans.
(3) be placed on and carry out in 95% alcohol liquid soaking in 72 hours, carry out ungrease treatment.
(4) be placed on and carry out in 90% alcohol liquid soaking in 24 hours, carry out processed.
(5) be placed on then in the pepsin solution of 3%--7% and soaked 24 hours, continue immersion 24 hours after changing identical new liquid.
(6) be placed in the restriction endonuclease solution of 2%--8% and soaked 36 hours, to resolve the outer fascia between the nerve fibre bundle.
(7) put into 5% papain and carry out rinsing.
(7) put into 5% papain,
(8) scrub the cotton-shaped parsing foreign material of removal with cell pyrolysis liquid.
(9) change in the alcohol liquid of 6% calcium hypochlorite and 40% sodium chloride and 54% and carried out rinsing 48 hours.
(10) changing to the further infiltration of 70% ethanol and 28% hydrogen peroxide bleachinges and dyeing and Preservation Treatment.
(11) dry after radiation sterilization is handled, carry out the long-time preservation in drying back.
One, sample survey:
Table one best-of-breed technology scheme optimization
| 3% pepsin solution | 6.4% pepsin solution | 7% pepsin solution | |
| 2% restriction endonuclease solution | The surface is slightly coarse adhesion, disengaging not exclusively naturally | The line smooth surface, no adhesion not exclusively breaks away from naturally | The surface is slightly coarse adhesion, disengaging not exclusively naturally |
| 6% restriction endonuclease solution | Tiny adhesion phenomenon is arranged, can not break away from fully naturally | The line smooth surface, no adhesion phenomenon breaks away from naturally | Tiny adhesion phenomenon is arranged, can not break away from fully naturally |
| 8% restriction endonuclease solution | The surface is slightly coarse adhesion, disengaging not exclusively naturally | The line smooth surface, no adhesion not exclusively breaks away from naturally | The surface is slightly coarse adhesion, disengaging not exclusively naturally |
As table one, by the contrast of sample survey, the situation contrast when sense organ comparison and actual peeling off are carried out in the variation of the variation of the concentration of pepsin solution and restriction endonuclease solution, the concentration of the preferred plan of described pepsin solution is 6.4%; The concentration of the preferred plan of described restriction endonuclease solution is 6%.
The contrast of table two the present invention and prior art
Two, zoopery
Zoopery of the present invention is to adopt the new zealand rabbit experiment that undergos surgery.
Table three the present invention contrasts prior art center line zygonema result of use
| The present invention | Manual extraction | Nylon wire | The casing line | |
| Skin is subcutaneous | The smooth nothing hair of wound defect | The line plucked, the hairiness defect can absorb | Do not absorb accidental infection. | Accidental rejection is difficult for absorbing. |
| Back flesh layer | Absorbing does not have cicatrix fully | Can absorb | Do not absorb, need broken line. | Can absorb, effect is slow. |
| Fistulation at the bottom of the stomach | Wei Quan More after 8 to 45 days, no cicatrix is not taken out stitches. | Absorb, cicatrix is arranged | Do not absorb, cicatrix is arranged. | Absorb slowly, effect is slow. |
| Uterus and intestinal | Horizontal X X formula is sewed up after 8 to 45 days, and wound coincide good seamless. | Can absorb, incisura is arranged. | Do not absorb, need broken line. | Can absorb, slightly incisura. |
As shown in Table 3, the stitching thread that adopts technical scheme of the present invention to make, by comparing with the casing line with nylon suture, it not only can be applied in the stitching of human body surface wound, also can be applied in the operation stitching at inside of human body position, it can all be absorbed by human body without broken line, and any rejection phenomenon can not occur; When the stitching thread of manual extraction is compared, though the stitching thread of manual extraction also can be accomplished absorption of human body and preliminary anti-rejection effect, but owing in its tissue, do not dispose its more in-house harmful tissue and residue, thereby some lighter rejection phenomenons also can occur.
As shown in Table 4, the stitching thread that adopts technical scheme of the present invention to make, to the stitching that undergos surgery of a plurality of different parts, fully demonstrate sutural result of use involved in the present invention by zoopery, can not observe the scar after Shang Kou More closes at some position.
The stitching thread zoopery stitching thread operation result of use that table four is involved in the present invention
| Operative site | Use the line record | Clinical follow | Effect | |
| Skin is subcutaneous | 8 centimetres at family's rabbit back left side hypodermal layer otch | With 3 millimeters of the continuous sewing needle distances of 3-0 line | Observe behind the first quarter moon, suture absorbs, and incisura is arranged slightly. | Skin absorbs is fast, does not have rejection. |
| The back | 10 centimetres at skin of back muscle otch | Sew up 2 millimeters of interruption needle gages continuously with N stitching thread 3-0 | Observed in 17 days not have to infect and do not have rejection. | Absorb good. |
| The vertebra both sides | Muscle is respectively made 8 centimetres of otch | Use the 4-0 suturing with thread management, 2 millimeters of needle gages. | Cut hypersorption in the tissues observed after 45 days open. | Can not find suture and edge of a knife incisura, do not have and infect. |
| The abdominal part xiphoid-process | Stomach fistulation stomach bottom otch is 2 centimetres under the chest xiphoid-process | Use the 5-0 suturing with thread management, 2 millimeters of needle gages. | Eye split line absorption after 45 days. | There is not rejection, no cicatrix. |
| Abdominal part is the next | Intestinal anastomosis, 2 centimetres of otch of colon | With 6-0 line interrupted suture, 2 millimeters of needle gages. | Observe cicatrix after 45 days and all do not have hypersorption. | Hypersorption does not have rejection. |
| Product is cutd open in the uterus | 3 centimetres of uterine incisions | Make 2 millimeters of continuous mattress suture needle gages with the 8-0 line. | Guan Cha More closes good no cicatrix after 45 days. | Do not have rejection, do not have and infect, hypersorption. |
Claims (2)
1. a production method of absorbable suture of mammal nerve fiber is characterized in that, this method may further comprise the steps:
(1) Adult Mammals is put to death, remove the peel after cutting away its afterbody, the nerve fibre bundle that separates its afterbody is removed behind the surperficial blood stains standby with flushing with clean water;
(2) standby nerve fibre bundle being put into Hank ' s liquid cleans;
(3) be placed on and carry out in 95% alcohol liquid soaking in 72 hours, carry out ungrease treatment;
(4) be placed on and carry out in 90% alcohol liquid soaking in 24 hours, carry out processed;
(5) be placed on then in the pepsin solution of 3%--7% and soaked 24 hours, continue immersion 24 hours after changing identical new liquid;
(6) be placed in the restriction endonuclease solution of 2%--8% and soaked 36 hours, to resolve the outer fascia between the nerve fibre bundle;
(7) put into 5% papain and carry out rinsing;
(8) do the wash the cotton-shaped parsing foreign material of removal with cell pyrolysis liquid;
(9) change in the alcohol liquid of 6% calcium hypochlorite and 40% sodium chloride and 54% and carried out rinsing 48 hours;
(10) changing to the further infiltration of 70% ethanol and 28% hydrogen peroxide bleachinges and dyeing and Preservation Treatment;
(11) be positioned in the mixed solution of 70% ethanol and 25% glycerol and 5% glacial acetic acid, long-period freshness preserving is deposited under 4 ℃ of temperature, perhaps dries after radiation sterilization is handled, and carries out the long-time preservation of drying.
2. production method of absorbable suture of mammal nerve fiber according to claim 1 is characterized in that: described pepsin solution is that concentration is 6.4%; Described restriction endonuclease is 6%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100791272A CN100408110C (en) | 2006-04-29 | 2006-04-29 | Production method of absorbable suture of mammal nerve fiber |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2006100791272A CN100408110C (en) | 2006-04-29 | 2006-04-29 | Production method of absorbable suture of mammal nerve fiber |
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| CN1879898A CN1879898A (en) | 2006-12-20 |
| CN100408110C true CN100408110C (en) | 2008-08-06 |
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| CNB2006100791272A Expired - Fee Related CN100408110C (en) | 2006-04-29 | 2006-04-29 | Production method of absorbable suture of mammal nerve fiber |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101927032A (en) * | 2010-08-04 | 2010-12-29 | 陈启忠 | Dyeing preparation method of surgical sutures produced by tail tendons of nutria |
| CN102188745B (en) * | 2011-04-19 | 2013-10-02 | 陈启忠 | Preparation method of medical suture thread by drawing nutria tail tendon |
| CN102698314A (en) * | 2012-06-07 | 2012-10-03 | 曾共魁 | Production process for medical special mampalon collagen suture lines |
| CN104841007B (en) * | 2015-06-10 | 2016-06-01 | 湖南然元医用高科技蛋白线有限公司 | A kind of suture and its preparation method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262959A (en) * | 2000-03-01 | 2000-08-16 | 郑思华 | Preparation method of coypu collagen operating suture |
| US20050043819A1 (en) * | 2002-09-27 | 2005-02-24 | Board Of Regents, The University Of Texas System | Cell-free tissue replacement for tissue engineering |
| CN1197631C (en) * | 2002-12-30 | 2005-04-20 | 中国皮革和制鞋工业研究院 | Construction method for skin tissue engineering rack containing epidermal growth factor |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1262959A (en) * | 2000-03-01 | 2000-08-16 | 郑思华 | Preparation method of coypu collagen operating suture |
| US20050043819A1 (en) * | 2002-09-27 | 2005-02-24 | Board Of Regents, The University Of Texas System | Cell-free tissue replacement for tissue engineering |
| CN1197631C (en) * | 2002-12-30 | 2005-04-20 | 中国皮革和制鞋工业研究院 | Construction method for skin tissue engineering rack containing epidermal growth factor |
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| CN1879898A (en) | 2006-12-20 |
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Effective date of registration: 20061103 Address after: The new Xiang Xin Sheng village of Shuangcheng city in Heilongjiang Province Applicant after: Heilongjiang Kehong Biotechnology Development Co., Ltd. Address before: The new Xiang Xin Sheng village of Shuangcheng city in Heilongjiang Province Applicant before: Shuangcheng Beilong Biomed-Tech. Development Co.,Ltd |
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Granted publication date: 20080806 Termination date: 20110429 |