CN100408110C - Method for preparing absorbable suture of mammal nerve fiber - Google Patents
Method for preparing absorbable suture of mammal nerve fiber Download PDFInfo
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- CN100408110C CN100408110C CNB2006100791272A CN200610079127A CN100408110C CN 100408110 C CN100408110 C CN 100408110C CN B2006100791272 A CNB2006100791272 A CN B2006100791272A CN 200610079127 A CN200610079127 A CN 200610079127A CN 100408110 C CN100408110 C CN 100408110C
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- 210000004126 nerve fiber Anatomy 0.000 title claims abstract description 20
- 241000124008 Mammalia Species 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title claims abstract description 8
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000835 fiber Substances 0.000 claims abstract description 18
- 102000057297 Pepsin A Human genes 0.000 claims abstract description 12
- 108090000284 Pepsin A Proteins 0.000 claims abstract description 12
- 229940111202 pepsin Drugs 0.000 claims abstract description 12
- 108090000526 Papain Proteins 0.000 claims abstract description 7
- 239000004365 Protease Substances 0.000 claims abstract description 7
- 229940055729 papain Drugs 0.000 claims abstract description 7
- 235000019834 papain Nutrition 0.000 claims abstract description 7
- 238000002791 soaking Methods 0.000 claims abstract description 7
- 210000004369 blood Anatomy 0.000 claims abstract description 5
- 239000008280 blood Substances 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000243 solution Substances 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 18
- 210000005036 nerve Anatomy 0.000 claims description 15
- 108091008146 restriction endonucleases Proteins 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 claims description 9
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 7
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 238000004321 preservation Methods 0.000 claims description 6
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 4
- 210000004027 cell Anatomy 0.000 claims description 4
- 210000003195 fascia Anatomy 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- ZKQDCIXGCQPQNV-UHFFFAOYSA-N Calcium hypochlorite Chemical compound [Ca+2].Cl[O-].Cl[O-] ZKQDCIXGCQPQNV-UHFFFAOYSA-N 0.000 claims description 3
- OYPRJOBELJOOCE-IGMARMGPSA-N Calcium-40 Chemical compound [40Ca] OYPRJOBELJOOCE-IGMARMGPSA-N 0.000 claims description 3
- 238000005520 cutting process Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 238000004043 dyeing Methods 0.000 claims description 3
- 238000009897 hydrogen peroxide bleaching Methods 0.000 claims description 3
- 238000007654 immersion Methods 0.000 claims description 3
- 230000008595 infiltration Effects 0.000 claims description 3
- 238000001764 infiltration Methods 0.000 claims description 3
- 238000000197 pyrolysis Methods 0.000 claims description 3
- 230000005855 radiation Effects 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 229960000583 acetic acid Drugs 0.000 claims description 2
- 239000012362 glacial acetic acid Substances 0.000 claims description 2
- 239000011259 mixed solution Substances 0.000 claims description 2
- 238000000605 extraction Methods 0.000 abstract description 5
- 238000001356 surgical procedure Methods 0.000 abstract description 4
- 241000283984 Rodentia Species 0.000 abstract description 3
- 238000005238 degreasing Methods 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- 238000000926 separation method Methods 0.000 abstract description 2
- 239000002699 waste material Substances 0.000 abstract description 2
- 102000004533 Endonucleases Human genes 0.000 abstract 1
- 108010042407 Endonucleases Proteins 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000005406 washing Methods 0.000 abstract 1
- 210000000589 cicatrix Anatomy 0.000 description 7
- 208000027418 Wounds and injury Diseases 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 5
- 206010052428 Wound Diseases 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 239000004677 Nylon Substances 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 210000003205 muscle Anatomy 0.000 description 3
- 229920001778 nylon Polymers 0.000 description 3
- 210000002784 stomach Anatomy 0.000 description 3
- 238000007920 subcutaneous administration Methods 0.000 description 3
- 230000003187 abdominal effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000002729 catgut Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
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- 238000002474 experimental method Methods 0.000 description 2
- 238000005457 optimization Methods 0.000 description 2
- 210000004291 uterus Anatomy 0.000 description 2
- 210000002417 xiphoid bone Anatomy 0.000 description 2
- 206010020112 Hirsutism Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241000700110 Myocastor coypus Species 0.000 description 1
- 241000283977 Oryctolagus Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 208000028990 Skin injury Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000003409 anti-rejection Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001072 colon Anatomy 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
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- 239000003292 glue Substances 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 230000003886 intestinal anastomosis Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 206010033675 panniculitis Diseases 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
- 210000000697 sensory organ Anatomy 0.000 description 1
- 238000009958 sewing Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000004304 subcutaneous tissue Anatomy 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
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- Materials For Medical Uses (AREA)
Abstract
The invention relates to a method for preparing absorbable suture of mammal nerve fiber, which comprises the steps of washing the nerve fiber bundle at the tail of rodent of adult vertebrale with clear water to remove surface blood stain, cleaning in hank's, degreasing and dehydrating with alcohol with different concentrations, and soaking in pepsin solution and endonuclease solution; rinsing in 5% papain; the invention realizes natural separation of the tail nerve fiber bundles to form hundreds of independent and complete fiber threads, which not only greatly improves the quantity of prepared suture threads and leads the extraction rate to be close to 100 percent, thereby avoiding the waste of raw material resources; the surface of the nerve fiber is smooth and burr-free, the characteristics of good elasticity and strong flexibility of the original mammal nerve fiber are kept, the harmful tissues in the original mammal nerve fiber are eliminated, the rejection phenomenon between a human body and a suture line after surgical suture is thoroughly eradicated, and the pain of a patient after the surgery is further relieved.
Description
Technical field
The present invention relates in a kind of medical supplies suture produce manufacture method, particularly relate to a kind of production method of absorbable suture of mammal nerve fiber.
Background technology
In the prior art when hospital handles skin injury and operate on operation, carry out multi-level stitching to skin and subcutaneous tissue and organ, for the stitching of skin surface, be to adopt the higher nylon suture of intensity mostly, after the affected part recovers from injury, can take out by taking out stitches; And for the stitching of internal layer, stitching thread can not be taken out again, the stitching thread that has only employing to be absorbed voluntarily by human body, in the prior art, absorbable stitching thread mainly just is to use special-purpose catgut, and special-purpose catgut has certain rejection phenomenon to human body, often causes the subcutaneous lump that occurs in affected part, brings extra painful and infringement to the patient; Along with progress of science and technology, it is found that the laboratory animal use standard of vertebra mammals rodent near human physical signs, particularly nutria adopts growth and subsides meeting property and the no lymphocyte that its glue unit cultivates as cyton, can not produce the wound rejection phenomenon, find its collagen fiber moderate length simultaneously, the thickness difference, the operation stitching that flexible and traction force meets internal organs uses.
At present, in the prior art, produce this stitching thread, be to adopt peel manually mostly from the method that extracts, this mode causes its coccygeal nerve collagen fiber surface to be subjected to mechanicalness through regular meeting and damages, and its smooth in appearance is destroyed, and former cross sectional shape is compelled to drawing-down, destroy intrinsic fibre structure, influence its result of use; Greatly reduce the product line rate of each afterbody simultaneously, approximately can only output 15%, remainder then all slatterns, and so then improved cost of material greatly, and staff's working environment is relatively poor, the labor intensity height, the labor productive rate is low.
Summary of the invention
The purpose of this invention is to provide a kind of production method of absorbable suture of mammal nerve fiber, by the technical scheme of enzymolysis separation mammalian nervous fiber, to remedy the deficiencies in the prior art.
Production method of absorbable suture of mammal nerve fiber of the present invention is to carry out according to the following steps:
(1) Adult Mammals is put to death, remove the peel after cutting away its afterbody, the nerve fibre bundle that separates its afterbody is removed behind the surperficial blood stains standby with flushing with clean water.
(2) standby nerve fibre bundle being put into Hank ' s liquid cleans.
(3) be placed on and carry out in 95% alcohol liquid soaking in 72 hours, carry out ungrease treatment.
(4) be placed on and carry out in 90% alcohol liquid soaking in 24 hours, carry out processed.
(5) be placed on then in the pepsin solution of 3%--7% and soaked 24 hours, continue immersion 24 hours after changing identical new liquid.
(6) be placed in the restriction endonuclease solution of 2%--8% and soaked 36 hours, to resolve the outer fascia between the nerve fibre bundle.
(7) put into 5% papain and carry out rinsing.
(8) do the wash the cotton-shaped parsing foreign material of removal with cell pyrolysis liquid.
(9) change in the alcohol liquid of 6% calcium hypochlorite and 40% sodium chloride and 54% and carried out rinsing 48 hours.
(10) changing to the further infiltration of 70% ethanol and 28% hydrogen peroxide bleachinges and dyeing and Preservation Treatment.
(11) be positioned in the mixed solution of 70% ethanol and 25% glycerol and 5% glacial acetic acid, long-period freshness preserving is deposited under 4 ℃ of temperature, also can dry after radiation sterilization is handled, and carries out the long-time preservation in drying back.
As optimization, the preferred plan of described pepsin solution is that concentration is 6.4%; Described restriction endonuclease is 6%.
After the nerve fibre bundle that the present invention gets afterbody is removed surperficial blood stains with flushing with clean water, be placed among the hank ' s and clean, can protect its cell and nerve, protect its activity, also can play degreasing simultaneously; Through the defat and the dehydration of variable concentrations ethanol, be placed in the pepsin solution and soak again, to resolve muscle, lax flesh is opened, and places and soaks in the restriction endonuclease solution to resolve the outer fascia between the nerve fibre bundle; Put into 5% papain and carry out rinsing, papain enters the local nerve fiber, nerve fiber is resolved disperses, by technical matters method of the present invention, realize that enzymolysis separates the technical scheme of Mus coccygeal nerve collagen fiber, make the tail nerve fibre bundle realize that nature breaks away from, and becomes the single of up to a hundred independent completions.
Production method of absorbable suture of mammal nerve fiber beneficial effect of the present invention is, the process for making of producing by the technical program, 1, not only improved greatly and produced this stitching thread quantity, made its extraction ratio approach 100%, thereby stopped the waste of raw material resources; 2, produce the stitching thread produced on outward appearance, smooth surface does not have burr, and the good springiness that has kept original coccygeal nerve fiber, the characteristics that pliability is strong, 3, dispose unfavorable composition and tissue in the original coccygeal nerve fiber, the stitching thread that makes this method produce to produce, be more prone to be absorbed by human body, accelerate and improved the wound healing ability of human body, 4, by experiment as can be known, the present invention produces the stitching thread of producing, eliminate the harmful tissue in the original coccygeal nerve fiber, thoroughly eradicated the rejection phenomenon between the human body and stitching thread behind the operation stitching, further alleviated the misery behind the corrective surgery.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
The embodiment of production method of absorbable suture of mammal nerve fiber of the present invention carries out according to the following steps:
(1) the vertebra mammals rodent that will grow up is put to death, and removes the peel after cutting away its afterbody, and the nerve fibre bundle that separates its afterbody is removed behind the surperficial blood stains standby with flushing with clean water.
(2) standby nerve fibre bundle being put into Hank ' s liquid cleans.
(3) be placed on and carry out in 95% alcohol liquid soaking in 72 hours, carry out ungrease treatment.
(4) be placed on and carry out in 90% alcohol liquid soaking in 24 hours, carry out processed.
(5) be placed on then in the pepsin solution of 3%--7% and soaked 24 hours, continue immersion 24 hours after changing identical new liquid.
(6) be placed in the restriction endonuclease solution of 2%--8% and soaked 36 hours, to resolve the outer fascia between the nerve fibre bundle.
(7) put into 5% papain and carry out rinsing.
(7) put into 5% papain,
(8) scrub the cotton-shaped parsing foreign material of removal with cell pyrolysis liquid.
(9) change in the alcohol liquid of 6% calcium hypochlorite and 40% sodium chloride and 54% and carried out rinsing 48 hours.
(10) changing to the further infiltration of 70% ethanol and 28% hydrogen peroxide bleachinges and dyeing and Preservation Treatment.
(11) dry after radiation sterilization is handled, carry out the long-time preservation in drying back.
One, sample survey:
Table one best-of-breed technology scheme optimization
3% pepsin solution | 6.4% pepsin solution | 7% pepsin solution | |
2% restriction endonuclease solution | The surface is slightly coarse adhesion, disengaging not exclusively naturally | The line smooth surface, no adhesion not exclusively breaks away from naturally | The surface is slightly coarse adhesion, disengaging not exclusively naturally |
6% restriction endonuclease solution | Tiny adhesion phenomenon is arranged, can not break away from fully naturally | The line smooth surface, no adhesion phenomenon breaks away from naturally | Tiny adhesion phenomenon is arranged, can not break away from fully naturally |
8% restriction endonuclease solution | The surface is slightly coarse adhesion, disengaging not exclusively naturally | The line smooth surface, no adhesion not exclusively breaks away from naturally | The surface is slightly coarse adhesion, disengaging not exclusively naturally |
As table one, by the contrast of sample survey, the situation contrast when sense organ comparison and actual peeling off are carried out in the variation of the variation of the concentration of pepsin solution and restriction endonuclease solution, the concentration of the preferred plan of described pepsin solution is 6.4%; The concentration of the preferred plan of described restriction endonuclease solution is 6%.
The contrast of table two the present invention and prior art
Two, zoopery
Zoopery of the present invention is to adopt the new zealand rabbit experiment that undergos surgery.
Table three the present invention contrasts prior art center line zygonema result of use
The present invention | Manual extraction | Nylon wire | The casing line | |
Skin is subcutaneous | The smooth nothing hair of wound defect | The line plucked, the hairiness defect can absorb | Do not absorb accidental infection. | Accidental rejection is difficult for absorbing. |
Back flesh layer | Absorbing does not have cicatrix fully | Can absorb | Do not absorb, need broken line. | Can absorb, effect is slow. |
Fistulation at the bottom of the stomach | Wei Quan More after 8 to 45 days, no cicatrix is not taken out stitches. | Absorb, cicatrix is arranged | Do not absorb, cicatrix is arranged. | Absorb slowly, effect is slow. |
Uterus and intestinal | Horizontal X X formula is sewed up after 8 to 45 days, and wound coincide good seamless. | Can absorb, incisura is arranged. | Do not absorb, need broken line. | Can absorb, slightly incisura. |
As shown in Table 3, the stitching thread that adopts technical scheme of the present invention to make, by comparing with the casing line with nylon suture, it not only can be applied in the stitching of human body surface wound, also can be applied in the operation stitching at inside of human body position, it can all be absorbed by human body without broken line, and any rejection phenomenon can not occur; When the stitching thread of manual extraction is compared, though the stitching thread of manual extraction also can be accomplished absorption of human body and preliminary anti-rejection effect, but owing in its tissue, do not dispose its more in-house harmful tissue and residue, thereby some lighter rejection phenomenons also can occur.
As shown in Table 4, the stitching thread that adopts technical scheme of the present invention to make, to the stitching that undergos surgery of a plurality of different parts, fully demonstrate sutural result of use involved in the present invention by zoopery, can not observe the scar after Shang Kou More closes at some position.
The stitching thread zoopery stitching thread operation result of use that table four is involved in the present invention
Operative site | Use the line record | Clinical follow | Effect | |
Skin is subcutaneous | 8 centimetres at family's rabbit back left side hypodermal layer otch | With 3 millimeters of the continuous sewing needle distances of 3-0 line | Observe behind the first quarter moon, suture absorbs, and incisura is arranged slightly. | Skin absorbs is fast, does not have rejection. |
The back | 10 centimetres at skin of back muscle otch | Sew up 2 millimeters of interruption needle gages continuously with N stitching thread 3-0 | Observed in 17 days not have to infect and do not have rejection. | Absorb good. |
The vertebra both sides | Muscle is respectively made 8 centimetres of otch | Use the 4-0 suturing with thread management, 2 millimeters of needle gages. | Cut hypersorption in the tissues observed after 45 days open. | Can not find suture and edge of a knife incisura, do not have and infect. |
The abdominal part xiphoid-process | Stomach fistulation stomach bottom otch is 2 centimetres under the chest xiphoid-process | Use the 5-0 suturing with thread management, 2 millimeters of needle gages. | Eye split line absorption after 45 days. | There is not rejection, no cicatrix. |
Abdominal part is the next | Intestinal anastomosis, 2 centimetres of otch of colon | With 6-0 line interrupted suture, 2 millimeters of needle gages. | Observe cicatrix after 45 days and all do not have hypersorption. | Hypersorption does not have rejection. |
Product is cutd open in the uterus | 3 centimetres of uterine incisions | Make 2 millimeters of continuous mattress suture needle gages with the 8-0 line. | Guan Cha More closes good no cicatrix after 45 days. | Do not have rejection, do not have and infect, hypersorption. |
Claims (2)
1. a production method of absorbable suture of mammal nerve fiber is characterized in that, this method may further comprise the steps:
(1) Adult Mammals is put to death, remove the peel after cutting away its afterbody, the nerve fibre bundle that separates its afterbody is removed behind the surperficial blood stains standby with flushing with clean water;
(2) standby nerve fibre bundle being put into Hank ' s liquid cleans;
(3) be placed on and carry out in 95% alcohol liquid soaking in 72 hours, carry out ungrease treatment;
(4) be placed on and carry out in 90% alcohol liquid soaking in 24 hours, carry out processed;
(5) be placed on then in the pepsin solution of 3%--7% and soaked 24 hours, continue immersion 24 hours after changing identical new liquid;
(6) be placed in the restriction endonuclease solution of 2%--8% and soaked 36 hours, to resolve the outer fascia between the nerve fibre bundle;
(7) put into 5% papain and carry out rinsing;
(8) do the wash the cotton-shaped parsing foreign material of removal with cell pyrolysis liquid;
(9) change in the alcohol liquid of 6% calcium hypochlorite and 40% sodium chloride and 54% and carried out rinsing 48 hours;
(10) changing to the further infiltration of 70% ethanol and 28% hydrogen peroxide bleachinges and dyeing and Preservation Treatment;
(11) be positioned in the mixed solution of 70% ethanol and 25% glycerol and 5% glacial acetic acid, long-period freshness preserving is deposited under 4 ℃ of temperature, perhaps dries after radiation sterilization is handled, and carries out the long-time preservation of drying.
2. production method of absorbable suture of mammal nerve fiber according to claim 1 is characterized in that: described pepsin solution is that concentration is 6.4%; Described restriction endonuclease is 6%.
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CNB2006100791272A CN100408110C (en) | 2006-04-29 | 2006-04-29 | Method for preparing absorbable suture of mammal nerve fiber |
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CNB2006100791272A CN100408110C (en) | 2006-04-29 | 2006-04-29 | Method for preparing absorbable suture of mammal nerve fiber |
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CN100408110C true CN100408110C (en) | 2008-08-06 |
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CNB2006100791272A Expired - Fee Related CN100408110C (en) | 2006-04-29 | 2006-04-29 | Method for preparing absorbable suture of mammal nerve fiber |
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Families Citing this family (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101927032A (en) * | 2010-08-04 | 2010-12-29 | 陈启忠 | Dyeing preparation method of surgical sutures produced by tail tendons of nutria |
CN102188745B (en) * | 2011-04-19 | 2013-10-02 | 陈启忠 | Preparation method of medical suture thread by drawing nutria tail tendon |
CN102698314A (en) * | 2012-06-07 | 2012-10-03 | 曾共魁 | Production process for medical special mampalon collagen suture lines |
CN104841007B (en) * | 2015-06-10 | 2016-06-01 | 湖南然元医用高科技蛋白线有限公司 | A kind of suture and its preparation method |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1262959A (en) * | 2000-03-01 | 2000-08-16 | 郑思华 | Preparation method of coypu collagen operating suture |
US20050043819A1 (en) * | 2002-09-27 | 2005-02-24 | Board Of Regents, The University Of Texas System | Cell-free tissue replacement for tissue engineering |
CN1197631C (en) * | 2002-12-30 | 2005-04-20 | 中国皮革和制鞋工业研究院 | Construction method for skin tissue engineering rack containing epidermal growth factor |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN1262959A (en) * | 2000-03-01 | 2000-08-16 | 郑思华 | Preparation method of coypu collagen operating suture |
US20050043819A1 (en) * | 2002-09-27 | 2005-02-24 | Board Of Regents, The University Of Texas System | Cell-free tissue replacement for tissue engineering |
CN1197631C (en) * | 2002-12-30 | 2005-04-20 | 中国皮革和制鞋工业研究院 | Construction method for skin tissue engineering rack containing epidermal growth factor |
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