CN100398113C - Nano selenium of Melatonin and its preparation method - Google Patents
Nano selenium of Melatonin and its preparation method Download PDFInfo
- Publication number
- CN100398113C CN100398113C CNB021384045A CN02138404A CN100398113C CN 100398113 C CN100398113 C CN 100398113C CN B021384045 A CNB021384045 A CN B021384045A CN 02138404 A CN02138404 A CN 02138404A CN 100398113 C CN100398113 C CN 100398113C
- Authority
- CN
- China
- Prior art keywords
- melatonin
- selenium
- preparation
- composition
- acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to selenium nanoparticle of melatonin, which is a composition prepared from melatonin and red selenium nanoparticle. The composition is prepared by the reaction of the melatonin and selenium compounds. Research on the structure, the constituent and the property of the composition by an infrared spectrometer, an ultraviolet spectrometer, a mass spectrometer, etc. indicates that the structure and the property of the melatonin are not changed, the red selenium nanoparticle is adsorbed and wrapped by the melatonin to form stable particles, and the microstructure forms a spherical shape or a rod shape. The physiological efficiency of the melatonin and the physiological efficiency of the selenium nanoparticle in the composition can be mutually promoted to exert synergistic effects. The present invention has better efficiency for adjusting immunity, promoting sleep, enhancing memory, delaying aging, preventing various diseases, such as cancer, cardiovascular diseases, senile dementia, etc. The preparation method is simple and practical, and the composition is suitable for industrialized production.
Description
One, technical field
The present invention relates to a kind of health food and preparation method thereof, exactly is a kind of melatonin nanometer selenium and preparation method thereof.
Two, background technology
1973, The World Health Organization (WHO) announced to the whole world: selenium is trace element essential in human and animal's life, and selenium supplement can effectively prevent multiple disease.
Selenium is the component of GPx in the organism (glutathion peroxidase), PHGPx (phospholipid hydrogen glutathion peroxidase), ID (5`-takes off the iodine enzyme) active center key, participate in the metabolism of body endoperoxide and iodine, regulate the level and the free radical level of iodine in the organism, but enhancing body diseases prevention, resistance against diseases.Selenium has the effect of aspects such as removing free radical, prophylaxis of cancer, face nourishing antidebilitation, the liver protecting, opposing harmful heavy metal, radioprotective, immunomodulating; selenium has remarkable effect to treatment hepatitis, arthritis, cataract, cardiovascular disease, lupus erythematosus, hemorrhagic fever, and selenium also has tangible auxiliary treatment effect to the chemicotherapy of tumour patient.
Disclose according to World Health Organization (WHO), the whole world has 2,000,000,000 populations (in state-owned 200,000,000 populations) to survive in scarce selenium environment approximately at present.Selenium supplement can effectively prevent and treat multiple disease, but a selenic marked feature be exactly between its nutrition dosage and the toxicity dose scope narrow, and selenic useful physiological action such as anticancer often depends on higher intake, so its effective use amount is difficult to control.For many years, people are studying the efficient selenium product of low toxicity always.Compare with inorganic selenium, it is higher that the absorbing of organic selenium is worth, and acute toxicity is less, is considered to selenium product preferably.Experimental study shows that nanometer selenium more is better than organic selenium in many aspects, demonstrates the efficient feature of low toxicity.The biological effectiveness of nanometer selenium is approved that health ministry approved nanometer selenium capsule is health food (No. the 134th, a hygienic food and health number 1998).
CN1184776A discloses a kind of preparation method of red elemental selenium, reduction selenium compound or Lycoperdon polymorphum Vitt, black simple substance selenium obtain protein attitude red elemental selenium or polypeptide attitude red elemental selenium in protein or polypeptide system (solution), obtain the red elemental selenium of biologically active again through separation.
Melatonin (Melatonin, MT claims melatonine) is the human brain depths as " pinus " excretory a kind of indole amine bormones of size as the strobile, so also the someone is its " melatonin ", " melatonin ".Pineal activity has the obvious periodic variation, and illumination can suppress the secretion of pineal hormone, and is dark then stimulate the secretion of pineal hormone.The nineteen fifty-nine scientist successfully isolates the material of the discolored frog skin of a kind of picture from the pinus extract of cattle, so its called after melatonin.In recent ten years, scientist has carried out a large amount of basic research to melatonin secretion, adjusting, model of action and mechanism, find that it is extremely extensive to the effect of human body, relate to many aspects such as biorhythm, hormone secretion, nerve-endocrine-immunomodulating, stress, aging course, tumor generation and free radical resisting damage, rising blood glucose, calmness, hypnosis, antiinflammatory, analgesia.Melatonin can have inhibitory action to the central nervous system, can safeguard circadian rule round the clock, hypnotic, and has an enhancing immune function of human body, suppress the growth of tumor cell, prevention and treatment cardiovascular and cerebrovascular disease play regulating action to human body growth promoter, sexual function and many organs.Melatonin is the strongest endogenous free radical scavenger of finding so far (antioxidant), can prevent the cellular oxidation damage, and its effect has surpassed known all substance in vivos.Melatonin can directly be eliminated hydroxy radical and peroxylradicals, suppresses lipid peroxidation, strengthens activities of antioxidant enzymes and antioxidase gene expression, can protect biomacromolecules such as nucleus DNA, membrane lipid, plasmosin to avoid oxidative damage.
The secretion of melatonin and rhythmicity thereof and age are closely related in the body, people's pinus after being born 3 months begins to secrete melatonin, preschool period peaks, with age growth, the secretion of melatonin is on a declining curve after adolescence, particularly after 35 years old, reduced 10-15% in average per 10 years, reduce to extremely low level during by 80 years old, the secretion difference at daytime and night disappears substantially, thereby causes sleep disordered and a series of functional disorders.Brain is subject to the attack of free radical most; because the toxicity free radical that brain produces in the oxidative metabolism process is all more than other any organs; melatonin then is of paramount importance " marketing one's own products ", a dynamical endogenous antioxidant in the brain, therefore brain is had protective effect.Go into the melatonin secretion level old age and lower, to the defence capability decline of free-radical oxidation damage, first-selected brain is undermined.Thus, melatonin levels reduces, sleep reduces is one of important symbol of human brain aging.Melatonin is a kind of important antiaging hormone, from external additional melatonin, can make intravital melatonin levels maintain young state, adjust and the recovery circadian rhythm, can not only add deep sleep, improve sleep quality, memory reinforcing, the more important thing is the functional status that to improve whole health, improve the quality of living, the process of slow down aging.The maximum difference of melatonin and other sleeping pill is, melatonin does not have addiction, non-evident effect, 1~2 of per os hora somni in evening (containing melatonin 1.5-3mg approximately), just can produce sleepiness in the general twenty or thirty minute, and melatonin loses usefulness automatically after the daybreak in the morning, does not also have tired tired sensation after getting up.
Depression, schizophrenia etc. also are related with melatonin, and melatonin content is in low-level among the patients serum.The A Ercimo disease is exactly a senile dementia, is one of the world's three big senile disease.Though the definite cause of disease of this disease can't be illustrated, certainly that brain neuroblastoma unit is lost in a large number is relevant with the lipid peroxidation process of free radical by cell membrane.Studies show that, all people who suffers from senile dementia, its melatonin secretion level obviously reduces, and this shows that antioxidation and free radical inhibitory action weaken greatly, and the cerebral tissue of this moment is subjected to the infringement of free radical toxicity easily.Melatonin can prevent the apoptosis in the nervous tissue, and alzheimer disease is had therapeutical effect.Melatonin can suppress the excessive generation of NO in the body, helps protecting immunologic liver injury.Melatonin can reduce the level of cholesterol and triglyceride in the blood, the pituicyte promoting sexual gland hormone be can suppress and metakentrin and follicle stimulating hormone discharged, excited pituitary prolactin and growth hormone secretion participate in human sexual maturity and reproductive behavio(u)r, promote adolescence to grow.
The melatonin safety is big, uses the tens of milligram of per kilogram of body weight in the zoopery, and oncotherapy is used also safety still of number gram every day.The external melatonin that is extensive use of keeps healthy, and does not occur the untoward reaction report so far, and at home, melatonin health product " melatonin " are popular.
People need take two kinds of health foods for Selenium Supplement and melatonin now.
Three, summary of the invention
Thinking of the present invention provides a kind of health food, just can make different crowd be able to Selenium Supplement and melatonin simultaneously.Problem to be solved is at first to prepare the stable selenium and the complex of melatonin.Selenium is the red nano attitude of biologically active in the complex, and nanoparticle is cladded by melatonin, is referred to as the melatonin nanometer selenium.
Technical scheme of the present invention is directly to use melatonin and selenium compound to prepare the melatonin nanometer selenium.
Described selenium compound can be selenite or acid selenite, such as sodium selenite (Na
2SeO
3), sodium hydroselenite (NaHSeO
3) etc., it reacts under the Reducing agent existence condition to redness with melatonin or its organic solution and no longer changes.
The first-selected vitamin C of described Reducing agent.
Described melatonin organic solution can be melatonin ethanol or acetone soln.
Described selenium compound can also be a seleno sulfate, such as sodium thiosulfate (Na
2SeSO
3) etc.It mixes the back with melatonin or its organic solution and transfers reactant pH to 5.5~7.5 with organic acid.
Described organic acid can be ascorbic acid (vitamin C) or citric acid etc.
Described melatonin organic solution can be melatonin ethanol or acetone soln.
Concerning selenium compound, can also use initiation material preparation selenium compound solution earlier, product directly is used for preparing the melatonin nanometer selenium without separation.
In the preparation, the relative quantity of two kinds of raw materials can be adjusted according to separately daily intaking amount scope, day clothes number of times etc., and can process series of products at the needs of different crowd.It is 3 milligrams (mg)/day to middle-aged and elderly people for improving sleep that the daily intaking amount of melatonin, China's Ministry of Public Health are ratified this health food.Melatonin went on the market so far from the eighties in 20th century, and clinical trial has widely all been done by each state, with regard to daily intaking amount, 5mg, 10mg, 20mg, 40mg, 80mg etc. is arranged.Up to the present, medicine management department in various countries' comprises that U.S. FDA and the EMEA of European Union all do not issue the report about melatonin safety issue or untoward reaction.
About selenic daily intaking amount, Chinese scholar Yang Guangqi professor and professor Chen Xiaoshu etc. are through result of study is as follows for many years: selenic physiological need amount 40 micrograms (μ g)/day, 800 μ g/ days of boundary toxic dose, suggestion the highest safe 400 μ g/ days of intake of meal supplement 50~250 μ g/ days of amount.These data are adopted by three international organizations of FAD/WHO/IAEA.
Many-sided comparative study shows the melatonin nanometer selenium to its structure, composition and character through infrared spectrometer, ultraviolet spectrometer, mass spectrograph etc., the structure and the character of melatonin all do not change, red nano selenium is wrapped to form stable particle by melatonin absorption, and the microstructure of particle is spherical or bar-shaped.
The physiological function of melatonin and nanometer selenium can be mutually promoted in the products obtained therefrom, the performance synergism, have the effect of multiple diseases such as better immunomodulating, hypnotic, hypermnesis, slow down aging and prophylaxis of cancer, cardiovascular disease, senile dementia, can be used as " melatonin selenium " product and come into the market.
The preparation method of the present invention's design is simple, suitability for industrialized production.Reaction is all carried out at normal temperatures and pressures, and when using initiation material, each step reaction does not all relate to the product separation process, only needs solvent is removed.The sulphite that exists in the product can prevent the product oxidation deterioration.
Four, the specific embodiment
1,0.1g sodium selenite or hydrogen selenite sodium solution are under agitation dropped in the alcoholic solution that contains the 2g melatonin, add vitamin C or its solution again, when the redness that is stirred to mixed liquor no longer changes distilling under reduced pressure slough solvent product.
2,0.1g sodium selenite or hydrogen selenite sodium solution evenly are sprayed onto in the 2g solid powdered melatonin form pastel, under agitation add vitamin C or its solution, no longer change to the pastel redness, vacuum drying is sloughed moisture content and is promptly got product.
3, in embodiment 1 or 2, also can use the reaction earlier of initiation material selenium dioxide, water, sodium hydroxide solution to obtain sodium selenite or hydrogen selenite sodium solution, under the Reducing agent existence condition, prepare the melatonin nanometer selenium with melatonin again.
4, with 0.1g sodium selenite or sodium hydroselenite, under agitation throw and contain in the acetone soln of 2g melatonin, add vitamin C or its solution again, be stirred to the reactant liquor redness and no longer change, solvent is sloughed in distilling under reduced pressure and moisture promptly gets product.
5,0.1g sodium selenite or sodium hydroselenite are ground after with 2g solid powdery melatonin mix homogeneously, add vitamin C or its solution, the mixture color reddens gradually, no longer changes until redness promptly to get product.If added is that the vitamin C aqueous solution then needs vacuum drying to slough moisture.
6,0.2g sodium thiosulfate solution is under agitation dropped in the ethanol or acetone soln that contains the 3g melatonin, slowly regulate pH to 5.5~7.5 with other edible organic acid such as ascorbic acid (vitamin C), citric acids, when being stirred to redness and no longer changing distilling under reduced pressure slough solvent product.
7,0.2g sodium thiosulfate solution directly evenly is sprayed onto formation thick-pasty mixture in the 3g solid state powder shape melatonin, regulate pastel to redness with other edible organic acid solns such as ascorbic acid or citric acid at last and no longer change, vacuum drying is sloughed moisture and is promptly got product.
8, in embodiment 6 and 7, can use initiation material directly to prepare complex without separating behind the preparation sodium thiosulfate earlier with melatonin.Here it is obtains sodium thiosulfate solution with sodium thiosulfate or sodium sulfite with the reaction of simple substance selenium earlier, perhaps obtains sodium thiosulfate solution with sodium selenite and elemental sulfur reaction.Directly be mixed with melatonin nanometer selenium (as embodiment 6) with melatonin ethanol or acetone soln, perhaps directly it be sprayed to preparation melatonin nanometer selenium (as embodiment 7) in the solid powdery melatonin with this sodium thiosulfate solution.
Claims (7)
1. melatonin nanometer selenium is characterized in that: reaction and precipitation obtain by melatonin or its organic solution and selenite or acid selenite or its aqueous solution, under the Reducing agent existence condition or mixes the melatonin of transferring pH to 5.5~7.5 and precipitation to obtain with organic acid in the back with the seleno sulfate liquor by melatonin or its organic solution adsorbs and wraps up the selenic melatonin nano selenium compound of red nano.
2. the preparation method of a melatonin nanometer selenium comprises and mixing and precipitation, it is characterized in that: by melatonin or its organic solution and selenite or acid selenite or its aqueous solution, react under the Reducing agent existence condition to redness and no longer change.
3. preparation method according to claim 2 is characterized in that: described organic solvent is ethanol or acetone.
4. according to claim 2 or 3 described preparation methoies, it is characterized in that: described Reducing agent is a vitamin C.
5. the preparation method of a melatonin nanometer selenium comprises and mixing and precipitation, it is characterized in that: mix the back with the seleno sulfate liquor by melatonin or its organic solution and transfer pH to 5.5~7.5 and redness no longer to change with organic acid.
6. preparation method according to claim 5 is characterized in that: described organic solvent is ethanol or acetone.
7. according to claim 5 or 6 described preparation methoies, it is characterized in that: described organic acid is citric acid or ascorbic acid.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB021384045A CN100398113C (en) | 2002-09-30 | 2002-09-30 | Nano selenium of Melatonin and its preparation method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB021384045A CN100398113C (en) | 2002-09-30 | 2002-09-30 | Nano selenium of Melatonin and its preparation method |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1415309A CN1415309A (en) | 2003-05-07 |
CN100398113C true CN100398113C (en) | 2008-07-02 |
Family
ID=4749462
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CNB021384045A Expired - Fee Related CN100398113C (en) | 2002-09-30 | 2002-09-30 | Nano selenium of Melatonin and its preparation method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN100398113C (en) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105520953A (en) | 2011-07-25 | 2016-04-27 | 香港理工大学 | Nano-selenium hydrosol functionalized by pleurotus tuber-regium polysaccharide with antitumor activity and preparation method thereof |
CN102511701A (en) * | 2011-12-26 | 2012-06-27 | 苏州硒谷科技有限公司 | Method for removing inorganic selenium in liquid environment |
CN104496954B (en) * | 2014-12-13 | 2017-04-12 | 象山商博电子商务有限公司 | Preparation method of functionalized nano-selenium |
CN104823668B (en) * | 2015-05-13 | 2017-05-10 | 浙江大学 | application of selenium in improving content of plant endogenous melatonin |
CN113974082A (en) * | 2021-10-29 | 2022-01-28 | 成都德洛森医学研究院(有限合伙) | Sleep-aiding soymilk and preparation method thereof |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1357317A (en) * | 2000-12-15 | 2002-07-10 | 上海四通纳米技术港有限公司 | Prepn of superfine Se particle |
-
2002
- 2002-09-30 CN CNB021384045A patent/CN100398113C/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1357317A (en) * | 2000-12-15 | 2002-07-10 | 上海四通纳米技术港有限公司 | Prepn of superfine Se particle |
Also Published As
Publication number | Publication date |
---|---|
CN1415309A (en) | 2003-05-07 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
JP4312402B2 (en) | Anti-depressant, anti-menopausal agent, anti-senile dementia agent, anti-Alzheimer agent | |
CN109820905A (en) | A kind of pharmaceutical composition and its application for treating insomnia | |
JP3035834B2 (en) | Propolis component-containing solid, its production method and use | |
CN100398113C (en) | Nano selenium of Melatonin and its preparation method | |
KR100945462B1 (en) | Food composition for detoxificating heavy metal and health food comprising the same | |
CN113208117A (en) | Composition containing NMN and taurine and application thereof | |
CN103271353B (en) | Liver protecting and health care composition and preparation method thereof | |
JP2004261146A (en) | Nutritive assistance food | |
CN1415241A (en) | Nano selenium of glucomannan and its preparation method | |
CN108159073A (en) | A kind of Calci-Mix | |
JP2008307018A (en) | Mulberry leave-containing modified feed | |
CN1628702A (en) | Method for preparing sleep assisting melatonin milk tablet | |
CN108464416A (en) | Natural toxin-expelling anti-oxidation health beverage | |
KR20050080501A (en) | A making method of health assistance food for high blood pressure, fatness, diabetes patients. | |
CN1241486C (en) | Method for preparing health-care milk powder with food sleeping-care function contg. meilatoning | |
KR20120119686A (en) | A functional beverage composition comprising l-arginine, vitamin c, vitamin b complex, vitamin a, vitamin e and potassium iodide as main ingredients | |
CN1171594C (en) | Composition containing pearl powder and ascorbic acid | |
CN106071211A (en) | A kind of nursery piglet compound feed containing high-activity lactic acid bacteria and preparation method thereof | |
JP2007210917A (en) | Fat cell-reducing agent, medicine, and food and beverage | |
RU2057463C1 (en) | Biologically active food addition | |
JP2004089004A (en) | Arginine and rna-containing nutritional supplement for complementary therapy | |
CN114747681A (en) | Growth-promoting health-care feed additive for pigs | |
JP2005333858A (en) | Health food composition | |
CN111484900A (en) | Medium-chain fatty acid and preparation method thereof | |
JP2023062717A (en) | Nmn-containing antiaging composition |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C17 | Cessation of patent right | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080702 Termination date: 20110930 |