CN100376247C - Preparation of bio-membrane in use for promoting wound healing, and preparation method - Google Patents
Preparation of bio-membrane in use for promoting wound healing, and preparation method Download PDFInfo
- Publication number
- CN100376247C CN100376247C CNB2004100096674A CN200410009667A CN100376247C CN 100376247 C CN100376247 C CN 100376247C CN B2004100096674 A CNB2004100096674 A CN B2004100096674A CN 200410009667 A CN200410009667 A CN 200410009667A CN 100376247 C CN100376247 C CN 100376247C
- Authority
- CN
- China
- Prior art keywords
- preparation
- wound healing
- oligochitosan
- propolis
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
The present invention relates to a biomembranous preparation used for promoting wound healing and a preparing method thereof, which is made by the mixture or one of 1 to 100 weight percentages of chitosan oligosaccharide, 0 to 50 weight percentages of sodium alginate and 0 to 49 weight percentages of propolis, wherein the polymerizing degree of the chitosan oligosaccharide is from 2 to 10, and the average molecular weight is from 500 to 3000. The specific preparing method is disclosed in the specification. The present invention has the advantages of the functions of wound healing promotion, hemostasis, sterilization, analgesia, etc., low cost, good effect and no side effect, and belongs to a natural product. The product can form a biologic protective film, and has an obvious improving function on injuries and wound healing. Meanwhile, the present invention also has excellent functions for removing free radicals, protecting cell membranes, improving microcirculation, promoting damaged tissue repair, etc.
Description
Technical field
The present invention relates to disposable sanitary articles, specifically relate to a kind of biomembranous preparation of promoting wound healing and preparation method thereof that is used to.
Background technology
Experiment confirm: pure natural product--propolis is the most responsive to gram positive bacteria, acid fast bacteria; Inhibitory action to staphylococcus aureus, viridans streptococci, Bacillus proteus, Hemolytic streptococcus is better than penicillin, tetracycline.Gram-negative meningococcus, pathogenic halophilic bacteria, NAG vibrios and helicobacter pylori etc. also there is strong antibacterial activity.Pathogenic mycoplasma there is strong inhibitory action.Diphtheria corynebacterium, clostridium tetani, bacillus oedematis-maligni extracellular toxin there is neutralization.
Calcium alginate is the polysaccharide material that refines from seaweed plants.When it is used for the wound contact layer, interact between it and the wound, can produce sodium alginate, calcium alginate gel.This gel is hydrophilic, oxygen is passed through and antibacterial can not pass through, and promotes neoblastic growth.Sodium ion in calcium alginate and wound transudate and the blood exchanges, and discharges calcium ion, and forms a gel thin-layer on the wound surface.Because the release of calcium ion has been quickened the formation of clot in the blood capillary end, thereby has been reached rapid hemostasis.Because the formation of wound surface gel layer is played the certain protection effect to wound surface, and make wound surface keep certain humidity, temperature is created a good microenvironment for the healing of wound surface, impels wound to heal early.
Oligochitosan is the specific aggregation degree biological activity segment material of a kind of marine polysaccharide through the degraded of modern biological project means.Oligochitosan is because of the water solublity height, and biocompatibility is good, easier being utilized, more remarkable effect.Being used for the hemostatic product for chitosan at present has many kinds, but utilizes the oligochitosan biological preparation to stop blooding, and has not yet to see report, finds through experiment: oligochitosan has good water-solubility, hemostasis and promotes the wound healing effect.This product has unique curative effect to wound healing, pain relieving preventing from scar and ablative aspect.Therefore, has tangible competitive advantage on the domestic and international market.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, and a kind of biomembranous preparation of wound healing and preparation method thereof that is used to promote is provided.
The object of the invention can realize by following measure: a kind of biomembranous preparation that is used to promote wound healing: by weight percentage, mixed or formed separately by oligochitosan 1-100%/sodium alginate 0-50%, propolis 0-49%; Wherein, the degree of polymerization of oligochitosan is 2-10, mean molecule quantity 500-3000; Preparation has two kinds of forms:
(1) powder
1). under the room temperature,, oligochitosan, sodium alginate, propolis powder are broken into fine powder 60-150 order, mixing in agitator by claim 1 raw materials by weight percent;
2). with above-mentioned powder, through 50-100 ℃, drying was sterilized 1-3 hour; Or radiation sterilization.
3). aseptic subpackaged, make capsule, tablet or the agent of dusting;
(2) water preparation
1). under the room temperature,, after in agitator, mixing, make the solution that concentration is 5%-30%w/v with deionized water dissolving by claim 1 raw materials by weight percent oligochitosan, sodium alginate and propolis;
2). after the aforesaid liquid bottling, through 50-100 ℃, moist heat sterilization 1-3 hour.
One the present invention has following advantage compared to existing technology:
1. the biomembrane of the present invention's exploitation has functions such as the wound healing of promotion, hemostasis, sterilization, analgesia, and cost is low, and effect is good, has no side effect, and belongs to natural prodcuts; Product can form one deck biological protection film, and wound and wound healing are had obvious improvement effect, has the removing free radical simultaneously, the protection cell membrane, and microcirculation improvement, promotion damaged tissue reparation etc. all has good effect.The health product of the present invention's prescription all are initiatives through international, domestic online information retrieval.
2. effect is good, has no side effect.Powder of the present invention and water preparation biomembrane antioxidation are removed free radical, the protection cell membrane, and microcirculation improvement promotes the damaged tissue reparation, has kept 26S Proteasome Structure and Function complete of film, transaminase lowering improves liver function all good effect.
3. the mechanism of action uniqueness during blood coagulation, because the activation of each thrombin has caused fibrinous formation in the blood plasma, reaches the hemostasis purpose.The haemostatic medicament that uses also all is by exciting thrombin, Blood clotting being taken place at present.Studies have shown that biomembranous blood clotting mechanism does not rely on above approach.Normal blood is removed fibrin, and biomembrane still can cause blood clotting in 40 seconds.Illustrate that the biomembrane hemostasis does not have dependency to protein in the blood.Blood is removed blood plasma, and after leukocyte, the platelet, biomembrane produced blood coagulation in 30 seconds.This illustrates that biomembranous anastalsis is relevant with erythrocyte.Stereoscan photograph shows that erythrocyte is attached on together mutually, and with the company of folding with because antigen determines different cause different, makes it that crosslinked erythrocyte that just forms take place in the biomembranous existence of erythrocyte surface and stick.In addition, biomembrane can also suppress fibrinolytic activity in the body, functions such as stimulating platelet activation.This explanation biomembrane is a kind of novel hemorrhage.
Biomembrane is easy to be degraded in the wound part, and this catabolite can be absorbed by superficial cell, promotes into fine microcell and novel capillary proliferation, shortens the wound healing process
The specific embodiment
Enumerate 15 embodiment below, with explanation the present invention, but the present invention is not only limited to these embodiment.
Embodiment 1
1). under the room temperature, 1 kilogram of oligochitosan, 50 kilograms of sodium alginates, 49 kilograms of propolis powder are broken into fine powder (60-150 order), mixing in agitator in proportion, vacuum drying obtains mixed dust formulation;
2). quantitatively packing (50 gram/bottle), packing, quality inspection.
3). with the above-mentioned biomembrane preparation that is packaged into bottle, through 50-100 ℃, dry sterilization 1-3 hour; Or radiation sterilization.
Embodiment 2
Under the room temperature, with the raw material of 80 kilograms of oligochitosans, 12 kilograms of sodium alginates, 8 kilograms of propolis replacement embodiment 1, all the other methods are with embodiment 1.
Embodiment 3
Under the room temperature, with the raw material of 100 kilograms of oligochitosans replacement embodiment 1, all the other methods are with embodiment 1.
Embodiment 4
Under the room temperature, with the raw material of 80 kilograms of oligochitosans, 20 kilograms of sodium alginates replacement embodiment 1, all the other methods are with embodiment 1.
Embodiment 5
Under the room temperature, with the raw material of 80 kilograms of oligochitosans, 20 kilograms of propolis replacement embodiment 1, all the other methods are with embodiment 1.
Embodiment 6
1). under the room temperature, 1 kilogram of oligochitosan, 50 kilograms of sodium alginates, 49 kilograms of propolis after the mixing, are dissolved in the deionized water of 2000 liters in agitator according to the above ratio, make the mixed solution that concentration is 5% (w/v).
2). with the quantitative packing of aforesaid liquid (100ml/ bottle), through 50-100 ℃, moist heat sterilization 1-3 hour.
Embodiment 7
The raw material consumption is identical with embodiment 6 with method, and different is to be dissolved in the deionized water of 500 liters, makes the mixed solution that concentration is 20% (w/v).
Embodiment 8
Under the room temperature, 80 kilograms of oligochitosans, 12 kilograms of sodium alginates, 8 kilograms of propolis are dissolved in the deionized water of 2000 liters after mixing in agitator according to the above ratio, make the mixed solution that concentration is 5% (w/v).
2). with the quantitative packing of aforesaid liquid (100m1/ bottle), through 50-100 ℃, moist heat sterilization 1-3 hour.
Embodiment 9
The raw material consumption is identical with embodiment 8 with method, and different is to be dissolved in the deionized water of 500 liters, makes the mixed solution that concentration is 20% (w/v).
Embodiment 10
Under the room temperature, 100 kilograms of oligochitosans are dissolved in the deionized water of 2000 liters, make the solution that concentration is 5% (w/v).
2). with the quantitative packing of aforesaid liquid (100m1/ bottle), through 50-100 ℃, moist heat sterilization 1-3 hour.
Embodiment 11
The raw material consumption is identical with embodiment 10 with method, and different is to be dissolved in the deionized water of 500 liters, makes the solution that concentration is 20% (w/v).
Embodiment 12
Under the room temperature, 80 kilograms of oligochitosans and 20 kilograms of sodium alginates are dissolved in the deionized water of 2000 liters after the mixing in agitator according to the above ratio, make the mixed solution that concentration is 5% (w/v).
2). with the quantitative packing of aforesaid liquid (100m1/ bottle), through 50-100 ℃, moist heat sterilization 1-3 hour.
Embodiment 13
The raw material consumption is identical with embodiment 12 with method, and different is to be dissolved in the deionized water of 500 liters, makes the mixed solution that concentration is 20% (w/v).
Embodiment 14
Under the room temperature, 80 kilograms of oligochitosans and 20 kilograms of propolis are dissolved in the deionized water of 2000 liters after the mixing in agitator according to the above ratio, make the mixed solution that concentration is 5% (w/v).
2). with the quantitative packing of aforesaid liquid (100ml/ bottle), through 50-100, moist heat sterilization 1-3 hour.
Embodiment 15
The raw material consumption is identical with embodiment 14 with method, and different is to be dissolved in the deionized water of 500 liters, makes the mixed solution that concentration is 20% (w/v).
Application examples 1
The experiment of biomembrane water preparation haemostatic effect
At the hemorrhage wound surface of the hind leg manufacturing 2x1cm of mice size,, be that contrast is stopped blooding simultaneously with the hospital gauze with the hemostasis of biomembrane water preparation, record bleeding stopping period (experiment repeats 6 times altogether), calculate average bleeding stopping period, the biomembrane bleeding stopping period is 23 seconds as a result, and hospital gauze is 65 seconds.
Application examples 2
The biomembrane powder promotes the experiment of wound healing effect
Select the healthy male rat of body weight 180-200g for use, with the depilation of its back, cause third degree burn then, one group is applied with the biomembrane powder and to control, and controls with aseptic hospital gauze is deposited for two groups, and the next day changes once, continuous 10 days.Put to death whole rat after the 18th day, get the newborn skin of local healing, survey the content of its hydroxyproline, cut after the crust wound surface area and carry out the tectology inspection, the result shows, the biomembrane group promotes collagen protein synthesis significantly better than the hospital gauze group, and microscopic examination is to the various composition well-growns of wound surface of biomembrane group.From ripe collagen fiber bulk density distributed degrees, the biomembrane group is also obviously greater than the hospital gauze group
More than experimental results show that biomembranous wound surface hemostasis, promote that healing properties is good, have good biocompatibility again, therefore, aspect trauma care, have good application prospects.
Claims (2)
1. a biomembranous preparation that is used to promote wound healing is characterized in that: by weight percentage, mixed and formed by oligochitosan 1-100%, sodium alginate 0-50%, propolis 0-49%; Wherein, the degree of polymerization of oligochitosan is 2-10, mean molecule quantity 500-3000.
2. the preparation method of the described preparation of claim 1, it is characterized in that: preparation has two kinds of forms:
(1) powder
1). under the room temperature,, oligochitosan, sodium alginate, propolis powder are broken into fine powder 60-150 order, mixing in agitator by claim 1 raw materials by weight percent;
2). with above-mentioned powder, through 50-100 ℃, drying was sterilized 1-3 hour; Or radiation sterilization;
3). aseptic subpackaged, make capsule, tablet or the agent of dusting;
(2) water preparation
1). under the room temperature, press claim 1 raw materials by weight percent, after oligochitosan, sodium alginate and propolis are mixed, make the solution that concentration is 5%-30%w/v in agitator with deionized water dissolving;
2). after the aforesaid liquid bottling, through 50-100 ℃, moist heat sterilization 1～3 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100096674A CN100376247C (en) | 2004-10-14 | 2004-10-14 | Preparation of bio-membrane in use for promoting wound healing, and preparation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB2004100096674A CN100376247C (en) | 2004-10-14 | 2004-10-14 | Preparation of bio-membrane in use for promoting wound healing, and preparation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1759841A CN1759841A (en) | 2006-04-19 |
| CN100376247C true CN100376247C (en) | 2008-03-26 |
Family
ID=36706148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB2004100096674A Expired - Fee Related CN100376247C (en) | 2004-10-14 | 2004-10-14 | Preparation of bio-membrane in use for promoting wound healing, and preparation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN100376247C (en) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102727580B (en) * | 2011-04-11 | 2015-12-16 | 苏州瑞美科生物技术有限公司 | A kind of pharmaceutical composition foot wounds to repair |
| CN102727578A (en) * | 2011-04-11 | 2012-10-17 | 苏州瑞美科生物技术有限公司 | Pharmaceutical composition for healing wounds generated by chemoradiotherapy |
| CN104940979A (en) * | 2015-06-17 | 2015-09-30 | 陈红 | Microporous chitosan oligosaccharide/sodium alginate hemostasis granule and preparation method thereof |
| CN107213806B (en) * | 2017-07-13 | 2018-03-06 | 广州达济医学科技有限公司 | A kind of filter membrane for filtrating leukocytes and preparation method thereof |
| CN108853165A (en) * | 2018-07-20 | 2018-11-23 | 赵春芳 | A kind of wound prevents preparation to scab and preparation method thereof in agglutination |
| CN110772544A (en) * | 2019-11-13 | 2020-02-11 | 嘉善鸿域科技咨询有限公司 | Propolis product and its application in apparatus and wound treatment |
| CN110812527A (en) * | 2019-11-13 | 2020-02-21 | 浙江晟健医疗健康产业发展有限公司 | Propolis composition, apparatus thereof and application thereof in wound treatment |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167366C (en) * | 2002-04-17 | 2004-09-22 | 苗九昌 | Chitosan collagen and calcium alginate compounded spongy biological dressing and its preparation process |
-
2004
- 2004-10-14 CN CNB2004100096674A patent/CN100376247C/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1167366C (en) * | 2002-04-17 | 2004-09-22 | 苗九昌 | Chitosan collagen and calcium alginate compounded spongy biological dressing and its preparation process |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1759841A (en) | 2006-04-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6868314B2 (en) | Wound dressing containing hyaluronic acid-calcium and polylysine and its manufacturing method | |
| Ouyang et al. | Construction of a composite sponge containing tilapia peptides and chitosan with improved hemostatic performance | |
| CA2351341C (en) | Collagen hemostatic foam | |
| ES2205237T3 (en) | USE OF OXIDIZED CELLULOSE AND ITS COMPLEXES OF THE SAME FOR THE CICATRIZATION OF CHRONIC WOUNDS. | |
| EP0356060B1 (en) | Wound filling compositions | |
| ES2882911T3 (en) | Haemostatic compositions | |
| Hawthorne et al. | Enhancing wound healing dressing development through interdisciplinary collaboration | |
| JPS5838216A (en) | Condensed blood plasma derivative | |
| KR20170093243A (en) | Flowable hemostatic composition | |
| JPH07500095A (en) | Hemostatic composition for local hemostasis | |
| WO2009076873A1 (en) | A biocompatible denatured starch sponge material | |
| CN107754005A (en) | Hemostatic composition and its manufacture method | |
| CN103816562A (en) | Rapid hemostatic product for war wounds and preparation method thereof | |
| AU2017310470B2 (en) | Hemostatic flowable | |
| CN111803704B (en) | Nursing hemostatic gel and preparation method thereof | |
| Yan et al. | A keratin/chitosan sponge with excellent hemostatic performance for uncontrolled bleeding | |
| Mercy et al. | Chitosan-derivatives as hemostatic agents: Their role in tissue regeneration | |
| EP2249879B1 (en) | Pharmaceutical composition, dressing and method for treating skin lesion, intermediate composition and process for preparing said dressing, and use of cerium salt associated with a collagen matrix | |
| JP2024056090A (en) | High concentrated protein compositions for preventing tissue adhesion | |
| CN100376247C (en) | Preparation of bio-membrane in use for promoting wound healing, and preparation method | |
| Emami et al. | Bioactive wound powders as wound healing dressings and drug delivery systems | |
| US8507652B2 (en) | Pharmaceutical composition, dressing and method for treating skin lesion, intermediate composition and process for preparing said dressing, and use of cerium salt associated with a collagen matrix | |
| RU2744694C2 (en) | Hemostatic compositions | |
| WO2017060821A1 (en) | Hemostatic composition | |
| CN108553681A (en) | A kind of hemostasis medical dressing and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20080326 Termination date: 20091116 |