CN100374543C - Semi-synthetic culture medium of cordyceps sineusis - Google Patents
Semi-synthetic culture medium of cordyceps sineusis Download PDFInfo
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- CN100374543C CN100374543C CNB2004100908751A CN200410090875A CN100374543C CN 100374543 C CN100374543 C CN 100374543C CN B2004100908751 A CNB2004100908751 A CN B2004100908751A CN 200410090875 A CN200410090875 A CN 200410090875A CN 100374543 C CN100374543 C CN 100374543C
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- cordyceps
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- aweto
- sineusis
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Abstract
The present invention discloses aweto semisynthetic liquid or an aweto semisynthetic solid medium. The aweto semisynthetic culture medium provided by the present invention comprises 12.0 to 20.0 g of carbon, 0.70 to 1.49 g of nitrogen, 1.0 to 1.5 g of potassium dihydrogen phosphate and 0.5 to 1.0 g of magnesium sulfate, and water is added to the mixture of the components until the volume of the miture reaches 1000 ml, wherein the carbon source is sucrose or glucose or maltose or lactose; the nitrogen source is yeast extract and peptone, or yeast extract and beef extract. The aweto semisynthetic culture medium of the present invention has specific composing components, the fermentation production of aweto mycelium by adopting the culture medium has the advantages of simple technology, no heavy metal pollution, high mycelium growth speed, high mycelium yield (can reach more than 23 g/l), stable product quality, etc. The present invention not only is suitable for aweto physiological and biochemical research, but also is suitable for aweto industrialized fermentation production.
Description
Technical field
The present invention relates to a kind of Chinese caterpillar fungus culture medium, particularly a kind of semi-synthetic culture medium of cordyceps sineusis.
Background technology
Cordyceps sinensis [Cordyceps sinensis (Berk.) Sacc.] is a kind of rare Chinese medicine and the high tonic of China's special product, belongs to mycota (Kingdom Fungi), Ascomycota (Ascomycota), Hypocreales (Hypocreales), Clavicipitaceae (Clavicipitaceae), Cordyceps [Cordyceps (Fr.) Link] in classification of organisms.The record of the relevant Cordyceps sinensis of China is with a long history, just runs after fame with " Cordyceps sinensis " in the essentials of Matea Medica of 1694 year's harvest books and has put down in writing its medicinal efficacy for " sweet putting down protected the lung kidney-nourishing, hemostasis and phlegm, phthisical cough." also clearly record of " new compilation of materia medica " (1757) " Cordyceps sinensis flavor is sweet flat, goes into lung, kidney two warps, protects the lung kidney-nourishing, mends marrow, hemostasis and phlegm, phthisical cough, and it is all good to control diaphragm disease." in the Pharmacopoeia of the People's Republic of China (version in 2000), then be loaded with: " the Cordyceps sinensis flavor is sweet, and is flat.Return lung, kidney channel; Function is for invigorating the lung and the kidney hemostasis and phlegm.Be used for chronic cough void and breathe heavily, the labor spitting of blood of coughing, impotence and seminal emission, soreness of waist and knee joint." modern study shows that Cordyceps sinensis has immunomodulatory, eliminating phlegm and relieving asthma, and to the multiple pharmacological effect such as protection of lesions of liver and kidney, at aspects such as antitumor, treatment renal failures application promise in clinical practice arranged also.But Cordyceps sinensis is Qinghai-Tibet distinctive fungi, and limit to the distribution region, and natural output is very limited, adds the destruction of long-term excessively collection and ecotope, and tangible atrophy trend has appearred in the distribution range of this valuable ingredient of traditional Chinese medicine and generation quantity.Because good curing and health-care effect, the domestic and international market increases day by day to the Cordyceps sinensis demand at present, costs an arm and a leg, and is continuing rise., also become and alleviate natural resources pressure to replace natural cordyceps by the fermentative production Cordyceps fungus powder, satisfy the very potential approach of people's demand.
Domestic in recent years many units have carried out the Study on artificial culture of Cordyceps sinensis, and what have has entered suitability for industrialized production, but fermention medium all adopts natural medium (referring to utilize animal, plant, microorganism or the formulated substratum of other natural and organic ingredients).Since the natural medium complicated component, each batch material nutrient component content instability, and fermenting process and quality product are wayward; Each nutritive ingredient of natural medium is indeterminate in addition, is unsuitable for the research of Cordyceps sinensis Physiology and biochemistry.
Summary of the invention
The purpose of this invention is to provide the semi-synthetic liquid or solid substratum of a kind of Cordyceps sinensis.
Semi-synthetic culture medium of cordyceps sineusis provided by the present invention, comprise 12.0-20.0 gram carbon (pressing the carbon element content that molecular weight calculates), 0.70-1.49 gram nitrogen (nitrogen element content that mensuration draws), 1.0-1.5 gram potassium primary phosphate and 0.5-1.0 gram sal epsom, water is settled to 1000 milliliters; Described carbon source is sucrose or glucose or maltose or lactose, and nitrogenous source is yeast extract and peptone or yeast extract and beef extract.
Wherein, yeast extract is that nitrogenous source can provide vitamin complex again.
Can be as required, the agar that adds the quality percentage composition and be 1.5-2.0% in described substratum obtains solid medium.
The one-tenth that described semi-synthetic culture medium of cordyceps sineusis is preferably by following quality proportioning is grouped into: sucrose 30.0-50.0 gram, peptone 5.0-10.0 gram, yeast extract 1.0-3.0 gram, potassium primary phosphate 1.0-1.5 gram, sal epsom 0.5-1.0 gram, water is settled to 1000 milliliters.Especially be preferably sucrose 50.0 grams, peptone 10.0 grams, yeast extract 3.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, water is settled to 1000 milliliters.Then add the agar that the quality percentage composition is 1.5-2.0% in the solid medium again.
Above-mentioned semi-synthetic culture medium of cordyceps sineusis all can be prepared according to a conventional method, and as above-mentioned quality proportion raw material is put into container, heating for dissolving mixes, and sterilization gets final product.
Semi-synthetic culture medium of cordyceps sineusis of the present invention, its moiety is clear and definite, the raw material stable and consistent of fermentation, leavened prod is quality controllable, utilizes this substratum fermentative production Cordyceps sinensis to have technology and simply, does not have heavy metal contamination, and thalli growth speed is fast, bacterium powder productive rate height (can reach more than the 23g/l), advantages such as constant product quality both had been suitable for the research of Cordyceps sinensis Physiology and biochemistry, also were suitable for the industrial fermentation production of Cordyceps sinensis.
Embodiment
Embodiment 1, utilize semi-synthetic culture medium of cordyceps sineusis to produce Cordyceps fungus powder
Will be from separation and purification on the natural cordyceps, and be that the bacterial strain of real Cordyceps strain inserts in semi-synthetic culture medium of cordyceps sineusis and the control medium through molecular biology identification, fermentative production bacterium powder according to a conventional method respectively.The result shows that the bacterium powder productive rate of semi-synthetic culture medium of cordyceps sineusis reaches 23g/l, and the bacterium powder productive rate of control medium only is 5.83g/l.Wherein, the prescription of semi-synthetic culture medium of cordyceps sineusis is sucrose 50.0 grams, peptone 10.0 grams, yeast extract 3.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, 1000 milliliters of distilled water.The contrast culture based formulas is glucose 30.0 grams, peptone 8.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, vitamins B
10.5mg, 1000 milliliters of distilled water.
Embodiment 2, utilize semi-synthetic culture medium of cordyceps sineusis to produce Cordyceps fungus powder
Will be from separation and purification on the natural cordyceps, and be that the bacterial strain of real Cordyceps strain inserts in semi-synthetic culture medium of cordyceps sineusis and the control medium through molecular biology identification, fermentative production bacterium powder according to a conventional method respectively.The result shows that the bacterium powder productive rate of semi-synthetic culture medium of cordyceps sineusis reaches 16g/l, and the bacterium powder productive rate of control medium only is 5.83g/l.Wherein, the prescription of semi-synthetic culture medium of cordyceps sineusis is glucose 50.0 grams, extractum carnis 5.0 grams, and yeast extract 3.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, 1000 milliliters of distilled water, bacterium powder productive rate reaches 16g/l.The contrast culture based formulas is glucose 30.0 grams, peptone 8.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, vitamins B
10.5mg, 1000 milliliters of distilled water.
Embodiment 3, utilize semi-synthetic culture medium of cordyceps sineusis to produce Cordyceps fungus powder
Will be from separation and purification on the natural cordyceps, and be that the bacterial strain of real Cordyceps strain inserts in semi-synthetic culture medium of cordyceps sineusis and the control medium through molecular biology identification, fermentative production bacterium powder according to a conventional method respectively.The result shows that the bacterium powder productive rate of semi-synthetic culture medium of cordyceps sineusis reaches 15g/l, and the bacterium powder productive rate of control medium only is 5.83g/l.Wherein, the prescription of semi-synthetic culture medium of cordyceps sineusis is maltose 50.0 grams, peptone 10.0 grams, yeast extract 1.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, 1000 milliliters of distilled water.The contrast culture based formulas is glucose 30.0 grams, peptone 8.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, vitamins B
10.5mg, 1000 milliliters of distilled water.
Claims (6)
1. a semi-synthetic culture medium of cordyceps sineusis is made up of carbon source, nitrogenous source, potassium primary phosphate, sal epsom and water; Wherein, carbon is the 12.0-20.0 gram, and nitrogen is the 0.70-1.49 gram, and potassium primary phosphate is the 1.0-1.5 gram, and sal epsom is the 0.5-1.0 gram, and water is settled to 1000 milliliters; Described carbon source is sucrose or glucose or maltose or lactose, and nitrogenous source is yeast extract and peptone or yeast extract and beef extract.
2. substratum according to claim 1 is characterized in that: the agar that adds the quality percentage composition and be 1.5-2.0% in described substratum obtains solid medium.
3. substratum according to claim 1 and 2 is characterized in that: described semi-synthetic culture medium of cordyceps sineusis is sucrose 30.0-50.0 gram, peptone 5.0-10.0 gram, yeast extract 1.0-3.0 gram, potassium primary phosphate 1-1.5 gram, sal epsom 0.5-1 gram, water is settled to 1000 milliliters.
4. substratum according to claim 3 is characterized in that: the agar that adds the quality percentage composition and be 1.5-2.0% in described substratum obtains solid medium.
5. substratum according to claim 3 is characterized in that: described semi-synthetic culture medium of cordyceps sineusis is sucrose 50.0 grams, peptone 10.0 grams, and yeast extract 3.0 grams, potassium primary phosphate 1.0 grams, sal epsom 0.5 gram, water is settled to 1000 milliliters.
6. substratum according to claim 5 is characterized in that: the agar that adds the quality percentage composition and be 1.5-2.0% in described substratum obtains solid medium.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100908751A CN100374543C (en) | 2004-11-16 | 2004-11-16 | Semi-synthetic culture medium of cordyceps sineusis |
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2004100908751A CN100374543C (en) | 2004-11-16 | 2004-11-16 | Semi-synthetic culture medium of cordyceps sineusis |
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| CN1775943A CN1775943A (en) | 2006-05-24 |
| CN100374543C true CN100374543C (en) | 2008-03-12 |
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| CNB2004100908751A Expired - Fee Related CN100374543C (en) | 2004-11-16 | 2004-11-16 | Semi-synthetic culture medium of cordyceps sineusis |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102154180B (en) * | 2011-02-18 | 2013-01-02 | 厦门大学 | Culture medium for microvesicle bacteria BS03 and preparation method thereof |
| CN105037469A (en) * | 2015-06-24 | 2015-11-11 | 中山鼎晟生物科技有限公司 | Method for extracting cordycepin from submerged fermentation liquid of cordyceps militaris |
| CN105272449B (en) * | 2015-09-30 | 2017-04-05 | 鄄城县守红食用菌种植有限公司 | North Chinese caterpillar fungus culture medium and the technique for planting Cordyceps militaris (L.) Link. using the culture medium |
| CN106497795A (en) * | 2016-10-12 | 2017-03-15 | 锬酃藏虫草生物科技(深圳)有限公司 | A kind of Cordyceps funguss seed culture medium containing Fructus Lycii and its application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078051C (en) * | 1998-07-21 | 2002-01-23 | 李健 | Method for preparing health-care food contg. chinese caterpillar fungus |
| CN1414091A (en) * | 2001-10-26 | 2003-04-30 | 李健 | New technology of culturing cordyceps sinensis using insect |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1078051C (en) * | 1998-07-21 | 2002-01-23 | 李健 | Method for preparing health-care food contg. chinese caterpillar fungus |
| CN1414091A (en) * | 2001-10-26 | 2003-04-30 | 李健 | New technology of culturing cordyceps sinensis using insect |
Non-Patent Citations (1)
| Title |
|---|
| 冬虫夏草液体培养基的筛选和液体发酵技术研究. 张伟.中国医学生物技术应用杂志. 2004 * |
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